1nu1

Crystal Structure of Mitochondrial Cytochrome bc1 Complexed with 2-nonyl-4-hydroxyquinoline N-oxide (NQNO)

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Ubiquinol-cytochrome C reductase complex core protein I, mitochondrial

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 22	Ubiquinol-cytochrome C reductase complex core protein 2, mitochondrial × 2 （P23004） Cytochrome b × 2 （P00157） cytochrome c1 × 2 （P00125） UBIQUINOL-CYTOCHROME C REDUCTASE IRON-SULFUR SUBUNIT, mitochondrial × 2 （P13272） Ubiquinol-cytochrome C reductase complex 14 kDa protein × 2 （P00129） Ubiquinol-cytochrome C reductase complex ubiquinone-binding protein QP-C × 2 （P13271） Ubiquinol-cytochrome C reductase complex 11 kDa protein × 2 （P00126） Ubiquinol-cytochrome C reductase 8 kDa protein × 2 （P13272） Ubiquinol-cytochrome C reductase complex 7.2 kDa protein × 2 （P00130） Ubiquinol-cytochrome C reductase complex 6.4 kDa protein × 2 （P07552） PROTOPORPHYRIN IX CONTAINING FE × 6 2-NONYL-4-HYDROXYQUINOLINE N-OXIDE × 2 FE2/S2 (INORGANIC) CLUSTER × 2 water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	UQCR1_BOVIN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–446； UniProt 35–480

Ubiquinol-cytochrome C reductase complex core protein 2, mitochondrial

OrganismNot specified

UniProt P23004

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 22	Ubiquinol-cytochrome C reductase complex core protein I, mitochondrial × 2 （P31800） Cytochrome b × 2 （P00157） cytochrome c1 × 2 （P00125） UBIQUINOL-CYTOCHROME C REDUCTASE IRON-SULFUR SUBUNIT, mitochondrial × 2 （P13272） Ubiquinol-cytochrome C reductase complex 14 kDa protein × 2 （P00129） Ubiquinol-cytochrome C reductase complex ubiquinone-binding protein QP-C × 2 （P13271） Ubiquinol-cytochrome C reductase complex 11 kDa protein × 2 （P00126） Ubiquinol-cytochrome C reductase 8 kDa protein × 2 （P13272） Ubiquinol-cytochrome C reductase complex 7.2 kDa protein × 2 （P00130） Ubiquinol-cytochrome C reductase complex 6.4 kDa protein × 2 （P07552） PROTOPORPHYRIN IX CONTAINING FE × 6 2-NONYL-4-HYDROXYQUINOLINE N-OXIDE × 2 FE2/S2 (INORGANIC) CLUSTER × 2 water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	UQCR2_BOVIN
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–439； UniProt 15–453

Cytochrome b

OrganismNot specified

UniProt P00157

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 22	Ubiquinol-cytochrome C reductase complex core protein I, mitochondrial × 2 （P31800） Ubiquinol-cytochrome C reductase complex core protein 2, mitochondrial × 2 （P23004） cytochrome c1 × 2 （P00125） UBIQUINOL-CYTOCHROME C REDUCTASE IRON-SULFUR SUBUNIT, mitochondrial × 2 （P13272） Ubiquinol-cytochrome C reductase complex 14 kDa protein × 2 （P00129） Ubiquinol-cytochrome C reductase complex ubiquinone-binding protein QP-C × 2 （P13271） Ubiquinol-cytochrome C reductase complex 11 kDa protein × 2 （P00126） Ubiquinol-cytochrome C reductase 8 kDa protein × 2 （P13272） Ubiquinol-cytochrome C reductase complex 7.2 kDa protein × 2 （P00130） Ubiquinol-cytochrome C reductase complex 6.4 kDa protein × 2 （P07552） PROTOPORPHYRIN IX CONTAINING FE × 6 2-NONYL-4-HYDROXYQUINOLINE N-OXIDE × 2 FE2/S2 (INORGANIC) CLUSTER × 2 water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CYB_BOVIN
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 1–379； UniProt 1–379

cytochrome c1

OrganismNot specified

UniProt P00125

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 22	Ubiquinol-cytochrome C reductase complex core protein I, mitochondrial × 2 （P31800） Ubiquinol-cytochrome C reductase complex core protein 2, mitochondrial × 2 （P23004） Cytochrome b × 2 （P00157） UBIQUINOL-CYTOCHROME C REDUCTASE IRON-SULFUR SUBUNIT, mitochondrial × 2 （P13272） Ubiquinol-cytochrome C reductase complex 14 kDa protein × 2 （P00129） Ubiquinol-cytochrome C reductase complex ubiquinone-binding protein QP-C × 2 （P13271） Ubiquinol-cytochrome C reductase complex 11 kDa protein × 2 （P00126） Ubiquinol-cytochrome C reductase 8 kDa protein × 2 （P13272） Ubiquinol-cytochrome C reductase complex 7.2 kDa protein × 2 （P00130） Ubiquinol-cytochrome C reductase complex 6.4 kDa protein × 2 （P07552） PROTOPORPHYRIN IX CONTAINING FE × 6 2-NONYL-4-HYDROXYQUINOLINE N-OXIDE × 2 FE2/S2 (INORGANIC) CLUSTER × 2 water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CY1_BOVIN
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain D； PDBConstruct 1–241； UniProt 1–241

UBIQUINOL-CYTOCHROME C REDUCTASE IRON-SULFUR SUBUNIT, mitochondrial

OrganismNot specified

UniProt P13272

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 22	Ubiquinol-cytochrome C reductase complex core protein I, mitochondrial × 2 （P31800） Ubiquinol-cytochrome C reductase complex core protein 2, mitochondrial × 2 （P23004） Cytochrome b × 2 （P00157） cytochrome c1 × 2 （P00125） Ubiquinol-cytochrome C reductase complex 14 kDa protein × 2 （P00129） Ubiquinol-cytochrome C reductase complex ubiquinone-binding protein QP-C × 2 （P13271） Ubiquinol-cytochrome C reductase complex 11 kDa protein × 2 （P00126） Ubiquinol-cytochrome C reductase complex 7.2 kDa protein × 2 （P00130） Ubiquinol-cytochrome C reductase complex 6.4 kDa protein × 2 （P07552） PROTOPORPHYRIN IX CONTAINING FE × 6 2-NONYL-4-HYDROXYQUINOLINE N-OXIDE × 2 FE2/S2 (INORGANIC) CLUSTER × 2 water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	UCRI_BOVIN
Isoform	—
PDB entities	5, 9
Chains and sequence ranges	Author chain E； PDBConstruct 1–196； UniProt 79–274 Author chain I； PDBConstruct 1–57； UniProt 1–57

Ubiquinol-cytochrome C reductase complex 14 kDa protein

OrganismNot specified

UniProt P00129

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 22	Ubiquinol-cytochrome C reductase complex core protein I, mitochondrial × 2 （P31800） Ubiquinol-cytochrome C reductase complex core protein 2, mitochondrial × 2 （P23004） Cytochrome b × 2 （P00157） cytochrome c1 × 2 （P00125） UBIQUINOL-CYTOCHROME C REDUCTASE IRON-SULFUR SUBUNIT, mitochondrial × 2 （P13272） Ubiquinol-cytochrome C reductase complex ubiquinone-binding protein QP-C × 2 （P13271） Ubiquinol-cytochrome C reductase complex 11 kDa protein × 2 （P00126） Ubiquinol-cytochrome C reductase 8 kDa protein × 2 （P13272） Ubiquinol-cytochrome C reductase complex 7.2 kDa protein × 2 （P00130） Ubiquinol-cytochrome C reductase complex 6.4 kDa protein × 2 （P07552） PROTOPORPHYRIN IX CONTAINING FE × 6 2-NONYL-4-HYDROXYQUINOLINE N-OXIDE × 2 FE2/S2 (INORGANIC) CLUSTER × 2 water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	UCR6_BOVIN
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain F； PDBConstruct 1–110； UniProt 1–110

Ubiquinol-cytochrome C reductase complex ubiquinone-binding protein QP-C

OrganismNot specified

UniProt P13271

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 22	Ubiquinol-cytochrome C reductase complex core protein I, mitochondrial × 2 （P31800） Ubiquinol-cytochrome C reductase complex core protein 2, mitochondrial × 2 （P23004） Cytochrome b × 2 （P00157） cytochrome c1 × 2 （P00125） UBIQUINOL-CYTOCHROME C REDUCTASE IRON-SULFUR SUBUNIT, mitochondrial × 2 （P13272） Ubiquinol-cytochrome C reductase complex 14 kDa protein × 2 （P00129） Ubiquinol-cytochrome C reductase complex 11 kDa protein × 2 （P00126） Ubiquinol-cytochrome C reductase 8 kDa protein × 2 （P13272） Ubiquinol-cytochrome C reductase complex 7.2 kDa protein × 2 （P00130） Ubiquinol-cytochrome C reductase complex 6.4 kDa protein × 2 （P07552） PROTOPORPHYRIN IX CONTAINING FE × 6 2-NONYL-4-HYDROXYQUINOLINE N-OXIDE × 2 FE2/S2 (INORGANIC) CLUSTER × 2 water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	UCRQ_BOVIN
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain G； PDBConstruct 1–81； UniProt 1–81

Ubiquinol-cytochrome C reductase complex 11 kDa protein

OrganismNot specified

UniProt P00126

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 22	Ubiquinol-cytochrome C reductase complex core protein I, mitochondrial × 2 （P31800） Ubiquinol-cytochrome C reductase complex core protein 2, mitochondrial × 2 （P23004） Cytochrome b × 2 （P00157） cytochrome c1 × 2 （P00125） UBIQUINOL-CYTOCHROME C REDUCTASE IRON-SULFUR SUBUNIT, mitochondrial × 2 （P13272） Ubiquinol-cytochrome C reductase complex 14 kDa protein × 2 （P00129） Ubiquinol-cytochrome C reductase complex ubiquinone-binding protein QP-C × 2 （P13271） Ubiquinol-cytochrome C reductase 8 kDa protein × 2 （P13272） Ubiquinol-cytochrome C reductase complex 7.2 kDa protein × 2 （P00130） Ubiquinol-cytochrome C reductase complex 6.4 kDa protein × 2 （P07552） PROTOPORPHYRIN IX CONTAINING FE × 6 2-NONYL-4-HYDROXYQUINOLINE N-OXIDE × 2 FE2/S2 (INORGANIC) CLUSTER × 2 water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	UCRH_BOVIN
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain H； PDBConstruct 1–78； UniProt 1–78

Ubiquinol-cytochrome C reductase complex 7.2 kDa protein

OrganismNot specified

UniProt P00130

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 22	Ubiquinol-cytochrome C reductase complex core protein I, mitochondrial × 2 （P31800） Ubiquinol-cytochrome C reductase complex core protein 2, mitochondrial × 2 （P23004） Cytochrome b × 2 （P00157） cytochrome c1 × 2 （P00125） UBIQUINOL-CYTOCHROME C REDUCTASE IRON-SULFUR SUBUNIT, mitochondrial × 2 （P13272） Ubiquinol-cytochrome C reductase complex 14 kDa protein × 2 （P00129） Ubiquinol-cytochrome C reductase complex ubiquinone-binding protein QP-C × 2 （P13271） Ubiquinol-cytochrome C reductase complex 11 kDa protein × 2 （P00126） Ubiquinol-cytochrome C reductase 8 kDa protein × 2 （P13272） Ubiquinol-cytochrome C reductase complex 6.4 kDa protein × 2 （P07552） PROTOPORPHYRIN IX CONTAINING FE × 6 2-NONYL-4-HYDROXYQUINOLINE N-OXIDE × 2 FE2/S2 (INORGANIC) CLUSTER × 2 water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	UCR10_BOVIN
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain J； PDBConstruct 1–62； UniProt 1–62

Ubiquinol-cytochrome C reductase complex 6.4 kDa protein

OrganismNot specified

UniProt P07552

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 22	Ubiquinol-cytochrome C reductase complex core protein I, mitochondrial × 2 （P31800） Ubiquinol-cytochrome C reductase complex core protein 2, mitochondrial × 2 （P23004） Cytochrome b × 2 （P00157） cytochrome c1 × 2 （P00125） UBIQUINOL-CYTOCHROME C REDUCTASE IRON-SULFUR SUBUNIT, mitochondrial × 2 （P13272） Ubiquinol-cytochrome C reductase complex 14 kDa protein × 2 （P00129） Ubiquinol-cytochrome C reductase complex ubiquinone-binding protein QP-C × 2 （P13271） Ubiquinol-cytochrome C reductase complex 11 kDa protein × 2 （P00126） Ubiquinol-cytochrome C reductase 8 kDa protein × 2 （P13272） Ubiquinol-cytochrome C reductase complex 7.2 kDa protein × 2 （P00130） PROTOPORPHYRIN IX CONTAINING FE × 6 2-NONYL-4-HYDROXYQUINOLINE N-OXIDE × 2 FE2/S2 (INORGANIC) CLUSTER × 2 water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	UCR11_BOVIN
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain K； PDBConstruct 1–56； UniProt 1–56

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1nu1
Deposition date deposition_date	2003-01-30
Structure title title	Crystal Structure of Mitochondrial Cytochrome bc1 Complexed with 2-nonyl-4-hydroxyquinoline N-oxide (NQNO)
Keywords keywords	;BC1, QCR, MEMBRANE PROTEIN, PROTON TRANSLOCATION, ELECTRON TRANSFER, PROTEASE, MPP, MITOCHONDRIAL PROCESSING PEPTIDASE, CYTOCHROME C1, CYTOCHROME B, RIESKE, IRON SULFUR PROTEIN, OXIDOREDUCTASE, 2-NONYL-4-HYDROXYQUINOLINE N-OXIDE (NQNO) ;; OXIDOREDUCTASE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1nu1__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1nu1__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

1nu1__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	54.87 Å
Rg (electron density)	53.98 Å
Total Rg	54.16 Å
Atom count	33328
Residues	4210
Excluded volume	594750 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`1nu1__assembly_1__model_1`	22-meric （22）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (15)

Entity 1 Entity 1 polymer

Description description	Ubiquinol-cytochrome C reductase complex core protein I, mitochondrial
Molecular weight formula_weight	49266.3 kDa
EC 编号 ec_number	1.10.2.2
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	16666

TATYAQALQSVPETQVSQLDNGLRVASEQSSQPTCTVGVWIDAGSRYESEKNNGAGYFVEHLAFKGTKNRPGNALEKEVESMGAHLNAYSTREHTAYYIKALSKDLPKAVELLADIVQNCSLEDSQIEKERDVILQELQENDTSMRDVVFNYLHATAFQGTPLAQSVEGPSENVRKLSRADLTEYLSRHYKAPRMVLAAAGGLEHRQLLDLAQKHFSGLSGTYDEDAVPTLSPCRFTGSQICHREDGLPLAHVAIAVEGPGWAHPDNVALQVANAIIGHYDCTYGGGAHLSSPLASIAATNKLCQSFQTFNICYADTGLLGAHFVCDHMSIDDMMFVLQGQWMRLCTSATESEVLRGKNLLRNALVSHLDGTTPVCEDIGRSLLTYGRRIPLAEWESRIAEVDARVVREVCSKYFYDQCPAVAGFGPIEQLPDYNRIRSGMFWLRF

Entity 2 Entity 2 polymer

Description description	Ubiquinol-cytochrome C reductase complex core protein 2, mitochondrial
Molecular weight formula_weight	46575.5 kDa
EC 编号 ec_number	1.10.2.2
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

SLKVAPKVKATEAPAGVPPHPQDLEFTRLPNGLVIASLENYAPASRIGLFIKAGSRYENSNNLGTSHLLRLASSLTTKGASSFKITRGIEAVGGKLSVTSTRENMAYTVECLRDDVDILMEFLLNVTTAPEFRRWEVAALQPQLRIDKAVALQNPQAHVIENLHAAAYRNALANSLYCPDYRIGKVTPVELHDYVQNHFTSARMALIGLGVSHPVLKQVAEQFLNIRGGLGLSGAKAKYHGGEIREQNGDSLVHAALVAESAAIGSAEANAFSVLQHVLGAGPHVKRGSNATSSLYQAVAKGVHQPFDVSAFNASYSDSGLFGFYTISQAASAGDVIKAAYNQVKTIAQGNLSNPDVQAAKNKLKAGYLMSVESSEGFLDEVGSQALAAGSYTPPSTVLQQIDAVADADVINAAKKFVSGRKSMAASGNLGHTPFIDEL

Entity 3 Entity 3 polymer

Description description	Cytochrome b
Molecular weight formula_weight	42620.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C

MTNIRKSHPLMKIVNNAFIDLPAPSNISSWWNFGSLLGICLILQILTGLFLAMHYTSDTTTAFSSVTHICRDVNYGWIIRYMHANGASMFFICLYMHVGRGLYYGSYTFLETWNIGVILLLTVMATAFMGYVLPWGQMSFWGATVITNLLSAIPYIGTNLVEWIWGGFSVDKATLTRFFAFHFILPFIIMAIAMVHLLFLHETGSNNPTGISSDVDKIPFHPYYTIKDILGALLLILALMLLVLFAPDLLGDPDNYTPANPLNTPPHIKPEWYFLFAYAILRSIPNKLGGVLALAFSILILALIPLLHTSKQRSMMFRPLSQCLFWALVADLLTLTWIGGQPVEHPYITIGQLASVLYFLLILVLMPTAGTIENKLLKW

Entity 4 Entity 4 polymer

Description description	cytochrome c1
Molecular weight formula_weight	27323.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D

SDLELHPPSYPWSHRGLLSSLDHTSIRRGFQVYKQVCSSCHSMDYVAYRHLVGVCYTEDEAKALAEEVEVQDGPNEDGEMFMRPGKLSDYFPKPYPNPEAARAANNGALPPDLSYIVRARHGGEDYVFSLLTGYCEPPTGVSLREGLYFNPYFPGQAIGMAPPIYNEVLEFDDGTPATMSQVAKDVCTFLRWAAEPEHDHRKRMGLKMLLMMGLLLPLVYAMKRHKWSVLKSRKLAYRPPK

Entity 5 Entity 5 polymer

Description description	UBIQUINOL-CYTOCHROME C REDUCTASE IRON-SULFUR SUBUNIT, mitochondrial
Molecular weight formula_weight	21640.6 kDa
EC 编号 ec_number	1.10.2.2
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

SHTDIKVPDFSDYRRPEVLDSTKSSKESSEARKGFSYLVTATTTVGVAYAAKNVVSQFVSSMSASADVLAMSKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTKKEIDQEAAVEVSQLRDPQHDLERVKKPEWVILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRKGPAPLNLEVPSYEFTSDDMVIVG

Entity 6 Entity 6 polymer

Description description	Ubiquinol-cytochrome C reductase complex 14 kDa protein
Molecular weight formula_weight	13371.2 kDa
EC 编号 ec_number	1.10.2.2
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

AGRPAVSASSRWLEGIRKWYYNAAGFNKLGLMRDDTIHENDDVKEAIRRLPENLYDDRVFRIKRALDLSMRQQILPKEQWTKYEEDKSYLEPYLKEVIRERKEREEWAKK

Entity 7 Entity 7 polymer

Description description	Ubiquinol-cytochrome C reductase complex ubiquinone-binding protein QP-C
Molecular weight formula_weight	9606.0 kDa
EC 编号 ec_number	1.10.2.2
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G

GRQFGHLTRVRHVITYSLSPFEQRAFPHYFSKGIPNVLRRTRACILRVAPPFVAFYLVYTWGTQEFEKSKRKNPAAYENDR

Entity 8 Entity 8 polymer

Description description	Ubiquinol-cytochrome C reductase complex 11 kDa protein
Molecular weight formula_weight	9189.1 kDa
EC 编号 ec_number	1.10.2.2
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H

GDPKEEEEEEEELVDPLTTVREQCEQLEKCVKARERLELCDERVSSRSQTEEDCTEELLDFLHARDHCVAHKLFNSLK

Entity 9 Entity 9 polymer

Description description	Ubiquinol-cytochrome C reductase 8 kDa protein
Molecular weight formula_weight	5824.8 kDa
EC 编号 ec_number	1.10.2.2
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I

MLSVAARSGPFAPVLSATSRGVAGALRPLVQAAVPATSESPVLDLKRSVLCRESLRG

Entity 10 Entity 10 polymer

Description description	Ubiquinol-cytochrome C reductase complex 7.2 kDa protein
Molecular weight formula_weight	7209.3 kDa
EC 编号 ec_number	1.10.2.2
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J

VAPTLTARLYSLLFRRTSTFALTIVVGALFFERAFDQGADAIYEHINEGKLWKHIKHKYENK

Entity 11 Entity 11 polymer

Description description	Ubiquinol-cytochrome C reductase complex 6.4 kDa protein
Molecular weight formula_weight	6568.7 kDa
EC 编号 ec_number	1.10.2.2
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K

MLTRFLGPRYRQLARNWVPTAQLWGAVGAVGLVWATDWRLILDWVPYINGKFKKDD

Entity 12 Entity 12 non-polymer

Description description	PROTOPORPHYRIN IX CONTAINING FE
Molecular weight formula_weight	616.5 kDa
Source method src_method	syn
Copy count entity_count	3

Entity 13 Entity 13 non-polymer

Description description	2-NONYL-4-HYDROXYQUINOLINE N-OXIDE
Molecular weight formula_weight	287.4 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 14 Entity 14 non-polymer

Description description	FE2/S2 (INORGANIC) CLUSTER
Molecular weight formula_weight	175.8 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 15 Entity 15 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	2

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6. Fold Classification (SCOP + CATH) 31 domains

SCOP 2.08 (16 domains)

Domain ID domain_id	d1nu1a1
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.185 — LuxS/MPP-like metallohydrolase
Superfamily Superfamily superfamily	d.185.1 — LuxS/MPP-like metallohydrolase
Family Family family	d.185.1.1 — MPP-like

Domain ID domain_id	d1nu1a2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.185 — LuxS/MPP-like metallohydrolase
Superfamily Superfamily superfamily	d.185.1 — LuxS/MPP-like metallohydrolase
Family Family family	d.185.1.1 — MPP-like

Domain ID domain_id	d1nu1b1
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.185 — LuxS/MPP-like metallohydrolase
Superfamily Superfamily superfamily	d.185.1 — LuxS/MPP-like metallohydrolase
Family Family family	d.185.1.1 — MPP-like

Domain ID domain_id	d1nu1b2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.185 — LuxS/MPP-like metallohydrolase
Superfamily Superfamily superfamily	d.185.1 — LuxS/MPP-like metallohydrolase
Family Family family	d.185.1.1 — MPP-like

Domain ID domain_id	d1nu1c1
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.32 — a domain/subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)
Superfamily Superfamily superfamily	f.32.1 — a domain/subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)
Family Family family	f.32.1.1 — a domain/subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)

Domain ID domain_id	d1nu1c2
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.21 — Heme-binding four-helical bundle
Superfamily Superfamily superfamily	f.21.1 — Transmembrane di-heme cytochromes
Family Family family	f.21.1.2 — Cytochrome b of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)

Domain ID domain_id	d1nu1d1
Class class	a — All alpha proteins
Fold Fold fold	a.3 — Cytochrome c
Superfamily Superfamily superfamily	a.3.1 — Cytochrome c
Family Family family	a.3.1.3 — Cytochrome bc1 domain

Domain ID domain_id	d1nu1d2
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.23 — Single transmembrane helix
Superfamily Superfamily superfamily	f.23.11 — Cytochrome c1 subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase), transmembrane anchor
Family Family family	f.23.11.1 — Cytochrome c1 subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase), transmembrane anchor

Domain ID domain_id	d1nu1e1
Class class	b — All beta proteins
Fold Fold fold	b.33 — ISP domain
Superfamily Superfamily superfamily	b.33.1 — ISP domain
Family Family family	b.33.1.1 — Rieske iron-sulfur protein (ISP)

Domain ID domain_id	d1nu1e2
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.23 — Single transmembrane helix
Superfamily Superfamily superfamily	f.23.12 — ISP transmembrane anchor
Family Family family	f.23.12.1 — ISP transmembrane anchor

Domain ID domain_id	d1nu1f_
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.27 — 14 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)
Superfamily Superfamily superfamily	f.27.1 — 14 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)
Family Family family	f.27.1.1 — 14 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)

Domain ID domain_id	d1nu1g_
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.23 — Single transmembrane helix
Superfamily Superfamily superfamily	f.23.13 — Ubiquinone-binding protein QP-C of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)
Family Family family	f.23.13.1 — Ubiquinone-binding protein QP-C of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)

Domain ID domain_id	d1nu1h_
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.28 — Non-heme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)
Superfamily Superfamily superfamily	f.28.1 — Non-heme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)
Family Family family	f.28.1.1 — Non-heme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)

Domain ID domain_id	d1nu1i_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.184 — Non-globular alpha+beta subunits of globular proteins
Superfamily Superfamily superfamily	d.184.1 — Non-globular alpha+beta subunits of globular proteins
Family Family family	d.184.1.3 — Ubiquinol-cytochrome c reductase 8 kDa protein

Domain ID domain_id	d1nu1j_
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.23 — Single transmembrane helix
Superfamily Superfamily superfamily	f.23.14 — Subunit X (non-heme 7 kDa protein) of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)
Family Family family	f.23.14.1 — Subunit X (non-heme 7 kDa protein) of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)

Domain ID domain_id	d1nu1k_
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.23 — Single transmembrane helix
Superfamily Superfamily superfamily	f.23.15 — Subunit XI (6.4 kDa protein) of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)
Family Family family	f.23.15.1 — Subunit XI (6.4 kDa protein) of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)

CATH v4.4 (15 domains)

Domain ID domain_id	1nu1A01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	830 — Cytochrome Bc1 Complex; Chain A, domain 1
Homologous superfamily homologous superfamily	10 — Metalloenzyme, LuxS/M16 peptidase-like

Domain ID domain_id	1nu1A02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	830 — Cytochrome Bc1 Complex; Chain A, domain 1
Homologous superfamily homologous superfamily	10 — Metalloenzyme, LuxS/M16 peptidase-like

Domain ID domain_id	1nu1B01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	830 — Cytochrome Bc1 Complex; Chain A, domain 1
Homologous superfamily homologous superfamily	10 — Metalloenzyme, LuxS/M16 peptidase-like

Domain ID domain_id	1nu1B02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	830 — Cytochrome Bc1 Complex; Chain A, domain 1
Homologous superfamily homologous superfamily	10 — Metalloenzyme, LuxS/M16 peptidase-like

Domain ID domain_id	1nu1C00
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	810 — Cytochrome Bc1 Complex; Chain C
Homologous superfamily homologous superfamily	10 — Cytochrome Bc1 Complex; Chain C

Domain ID domain_id	1nu1D01
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily	100 — Cytochrome c1, transmembrane anchor, C-terminal

Domain ID domain_id	1nu1D02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	760 — Cytochrome Bc1 Complex; Chain D, domain 2
Homologous superfamily homologous superfamily	10 — Cytochrome c-like domain

Domain ID domain_id	1nu1E01
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily	270 — Ubiquinol cytochrome reductase, transmembrane domain

Domain ID domain_id	1nu1E02
Class class	2 — Mainly Beta
Architecture architecture	102 — 3-layer Sandwich
Topology topology	10 — Rieske Iron-sulfur Protein
Homologous superfamily homologous superfamily	10 — Rieske [2Fe-2S] iron-sulphur domain

Domain ID domain_id	1nu1F00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1090 — Cytochrome Bc1 Complex; Chain F
Homologous superfamily homologous superfamily	10 — Cytochrome b-c1 complex subunit 7

Domain ID domain_id	1nu1G00
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily	210 — Cytochrome b-c1 complex subunit 8

Domain ID domain_id	1nu1H00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	287 — Helix Hairpins
Homologous superfamily homologous superfamily	20 — Ubiquinol-cytochrome C reductase hinge domain

Domain ID domain_id	1nu1I00
Class class	2 — Mainly Beta
Architecture architecture	10 — Ribbon
Topology topology	210 — Cytochrome Bc1 Complex; Chain I
Homologous superfamily homologous superfamily	10 — Cytochrome Bc1 Complex; Chain I

Domain ID domain_id	1nu1J00
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily	260 — Cytochrome b-c1 complex subunit 9

Domain ID domain_id	1nu1K00
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily	220 —

▶

Unit-cell length a length_a	153.842 Å
Unit-cell length b length_b	153.842 Å
Unit-cell length c length_c	590.374 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	16

Space group space_group	I 41 2 2
Space-group number int_tables_number	98
Crystal system crystal_system	—

Resolution resolution	3.200 Å
Low-resolution limit resolution_low	10.000 Å
R factor r_factor	0.2173
R_work r_work	0.2148
R_free r_free	0.2955

Crystal Structure of Mitochondrial Cytochrome bc1 Complexed with 2-nonyl-4-hydroxyquinoline N-oxide (NQNO)

1. Protein Identity and Related Structures Protein Identity & Related Structures

Ubiquinol-cytochrome C reductase complex core protein I, mitochondrial

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Ubiquinol-cytochrome C reductase complex core protein 2, mitochondrial

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Cytochrome b

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

cytochrome c1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

UBIQUINOL-CYTOCHROME C REDUCTASE IRON-SULFUR SUBUNIT, mitochondrial

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Ubiquinol-cytochrome C reductase complex 14 kDa protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Ubiquinol-cytochrome C reductase complex ubiquinone-binding protein QP-C

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Ubiquinol-cytochrome C reductase complex 11 kDa protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Ubiquinol-cytochrome C reductase complex 7.2 kDa protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Ubiquinol-cytochrome C reductase complex 6.4 kDa protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer