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9q4d

A DARPin fused to the double trigger 1TEL variant crystallization chaperone via a direct helical fusion

Method: X-RAY DIFFRACTION D_max: 75.1 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9q4d
deposition_date	2025-08-20
last_revision	2025-10-01
title	A DARPin fused to the double trigger 1TEL variant crystallization chaperone via a direct helical fusion
keywords	TELSAM, DARPin, ETV6, Designed Ankyrin, Repeat Protein, Double Trigger, Protein Crystallization Chaperone, BIOSYNTHETIC PROTEIN; BIOSYNTHETIC PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	22.09 Å
rg_electron	21.55 Å
i0	11187400.00
molecular_weight	24758.0 kDa
excluded_volume	30811 Å³
envelope_volume	37134 Å³
shell_volume	15720 Å³
envelope_diameter	78.6 Å
shell_rg	26.40 Å
envelope_rg	21.69 Å
shape_rg	21.57 Å
total_rg	22.20 Å
total_atoms	3402
n_residues	226
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	75.1 Å
rg_real	22.30 Å
rg_real_error	0.74 Å
i0_real	1.1190e+07
i0_real_error	1.6420e+05
rg_reciprocal	22.26 Å
i0_reciprocal	11190000.0000
total_estimate	0.7486
solution_quality	REASONABLE a REASONABLE solution
n_peaks	3
r_peak_primary	19.1 Å
skewness	0.481
kurtosis	-0.412
angular_range	— – 0.3600 Å⁻¹
current_alpha	0.0000
highest_alpha	3444000.0000
n_real_points	68
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.706; Stabil: 0.992; Sysdev: 1.000; Positv: 1.000; Valcen: 0.634; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	Transcription factor ETV6,DARPin
formula_weight	27095.3 kDa
mutation	L96E, V112E in ETV6 (Uniprot numbering)
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	3622

MGHHHHHHHHHHSIRLPAHLRLQPIYWSRDDVAQWLKWAENEFSLRPIDSNTFEMNGKALLELTKEDFRYRSPHSGDELYELLQHILLGKKLLEAARAGQDDEVRILMANGADVNATDNDGYTPLHLAASNGHLEIVEVLLKNGADVNASDLTGITPLHAAAATGHLEIVEVLLKHGADVNAYDNDGHTPLHLAAKYGHLEIVEVLLKHGADVNAQDKFGKTAFDISIDNGNEDLAEILQKLN

Source

organism	synthetic construct
ncbi_taxonomy_id	32630
host_organism	Escherichia coli B

Entity 2 non-polymer

description	1,2-ETHANEDIOL
formula_weight	62.1 kDa
src_method	syn
entity_count	7

Entity 3 non-polymer

description	CHLORIDE ION
formula_weight	35.5 kDa
src_method	syn
entity_count	4

Entity 4 non-polymer

description	POTASSIUM ION
formula_weight	39.1 kDa
src_method	syn
entity_count	1

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	187

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6. Citations (1)

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length_a	83.021 Å
length_b	83.021 Å
length_c	56.840 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 65
int_tables_number	170
crystal_system	hexagonal

resolution	1.302 Å
resolution_low	35.950 Å
r_factor	0.1580
r_work	0.1562
r_free	0.1854

fit	/app/data/saxs_computation/q4/9q4d/9q4d.fit
pddf	/app/data/pr_computation/q4/9q4d/9q4d.pr.out
profile	/app/data/saxs_computation/q4/9q4d/9q4d.dat