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9qf2

Cryo-EM structure of the fully Coronin-1B-decorated actin filament in the ADP state.

Method: ELECTRON MICROSCOPY D_max: 185.2 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9qf2
deposition_date	2025-03-11
title	Cryo-EM structure of the fully Coronin-1B-decorated actin filament in the ADP state.
keywords	actin, coronin, filament, cytoskeleton, STRUCTURAL PROTEIN; STRUCTURAL PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	54.48 Å
rg_electron	54.43 Å
i0	2600780000.00
molecular_weight	425580.0 kDa
excluded_volume	531400 Å³
envelope_volume	717920 Å³
shell_volume	108950 Å³
envelope_diameter	200.8 Å
shell_rg	57.73 Å
envelope_rg	54.16 Å
shape_rg	54.47 Å
total_rg	54.41 Å
total_atoms	29890
n_residues	3790
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	185.2 Å
rg_real	54.48 Å
rg_real_error	2.29 Å
i0_real	2.6010e+09
i0_real_error	6.0890e+07
rg_reciprocal	54.47 Å
i0_reciprocal	2601000000.0000
total_estimate	0.8795
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	63.9 Å
skewness	0.320
kurtosis	-0.430
angular_range	— – 0.1450 Å⁻¹
current_alpha	0.0000
highest_alpha	319000000.0000
n_real_points	30
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.863; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.841

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	Actin, cytoplasmic 1, N-terminally processed
formula_weight	41632.4 kDa
mutation	C272A
src_method	man
entity_count	5

Polymer

type	polypeptide(L)
strand_id	C,A,B,D,E
atom_count	30562

DDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Trichoplusia ni

Entity 2 polymer

description	Coronin-1B,Methylated-DNA--protein-cysteine methyltransferase
formula_weight	74788.1 kDa
ec_number	2.1.1.63
src_method	man
entity_count	5

Polymer

type	polypeptide(L)
strand_id	H,F,G,I,J

GAMGSMSFRKVVRQSKFRHVFGQPVKNDQCYEDIRVSRVTWDSTFCAVNPKFLAVIVEASGGGAFLVLPLSKTGRIDKAYPTVCGHTGPVLDIDWCPHNDEVIASGSEDCTVMVWQIPENGLTSPLTEPVVVLEGHTKRVGIIAWHPTARNVLLSAGCDNVVLIWNVGTAEELYRLDSLHPDLIYNVSWNHNGSLFCSACKDKSVRIIDPRRGTLVAEREKAHEGARPMRAIFLADGKVFTTGFSRMSERQLALWDPENLEEPMALQELDSSNGALLPFYDPDTSVVYVCGKGDSSIRYFEITEEPPYIHFLNTFTSKEPQRGMGSMPKRGLEVSKCEIARFYKLHERKCEPIVMTVPRKSDLFQDDLYPDTAGPEAALEAEEWVSGRDADPILISLREAYVPSKQRDLKISRRNVLSDSRPAMAPGSSHLGAPASTTTAADATPSGSLARAGEAGKLEEVMQELRALRALVKEQGDRICRLEEQLGRMENGDAGTGGGGSGGGGSMDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVEVPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLAVKEWLLAHEGHRLGKPGLG

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Trichoplusia ni

Entity 3 non-polymer

description	ADENOSINE-5'-DIPHOSPHATE
formula_weight	427.2 kDa
src_method	syn
entity_count	5

Entity 4 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	5

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	672

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	2.42 Å
resolution_method	—
num_particles	608622

fit	/app/data/saxs_computation/qf/9qf2/9qf2.fit
pddf	/app/data/pr_computation/qf/9qf2/9qf2.pr.out
profile	/app/data/saxs_computation/qf/9qf2/9qf2.dat

Cryo-EM structure of the fully Coronin-1B-decorated actin filament in the ADP state.

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 non-polymer

Entity 5 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)