Current Protein Identity：B7MGC4
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 10QM E.coli 50S ribosomal subunit bound to compound 48a Deposited 2026-02-01 | Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric（31） Consistent with all polymers |
Chain k
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 2.49 Å |
| 11GG Chimeric Escherichia coli 70S ribosome containing an evolved 16S rRNA from Pseudomonas aeruginosa (PA-ST) Deposited 2026-02-22 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain k
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 307 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.02 Å |
| 5U9F 3.2 A cryo-EM ArfA-RF2 ribosome rescue complex (Structure II) Deposited 2016-12-16 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain 32
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 360 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 5U9G 3.2 A cryo-EM ArfA-RF2 ribosome rescue complex (Structure I) Deposited 2016-12-16 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain 32
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 348 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 5WF0 70S ribosome-EF-Tu H84A complex with GTP and near-cognate tRNA (Complex C2) Deposited 2017-07-11 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain 2
1–45（45 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 1480 K POTASSIUM ION × 5 PHE PHENYLALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；5 second blot time,
no wait time
|
Resolution 3.60 Å |
| 5WFS 70S ribosome-EF-Tu H84A complex with GTP and near-cognate tRNA (Complex C4) Deposited 2017-07-12 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain 2
1–45（45 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 1471 K POTASSIUM ION × 7 PHE PHENYLALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；5 second blot time,
no wait time
|
Resolution 3.00 Å |
| 6DNC E.coli RF1 bound to E.coli 70S ribosome in response to UAU sense A-site codon Deposited 2018-06-06 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain HA
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7；20 mM Tris-HCl, 100 mM NH4Cl, 20.5 mM MgCl2, 0.5 mM EDTA, 6 mM beta-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6O9J 70S Elongation Competent Ribosome Deposited 2019-03-14 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 3
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6O9K 70S initiation complex Deposited 2019-03-14 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 7
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 6OGF 70S termination complex with RF2 bound to the UGA codon. Partially rotated ribosome with RF2 bound (Structure III). Deposited 2019-04-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain D
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6OGG 70S termination complex with RF2 bound to the UGA codon. Rotated ribosome with RF2 bound (Structure IV). Deposited 2019-04-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain D
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6OGI 70S termination complex with RF2 bound to the UAG codon. Rotated ribosome conformation (Structure V) Deposited 2019-04-02 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain D
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 6OM6 Structure of trans-translation inhibitor bound to E. coli 70S ribosome with P site tRNA Deposited 2019-04-18 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | KKL 4-amino-N-[5-(4-ethynylphenyl)-1,3,4-oxadiazol-2-yl]benzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Blot time: 3.5 seconds
|
Resolution 3.10 Å |
| 6ORE Release complex 70S Deposited 2019-04-30 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 436 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.90 Å |
| 6ORL RF1 pre-accommodated 70S complex at 24 ms Deposited 2019-04-30 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 295 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.50 Å |
| 6OSK RF1 accommodated 70S complex at 60 ms Deposited 2019-05-01 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 307 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.60 Å |
| 6OSQ RF1 accommodated state bound Release complex 70S at long incubation time point Deposited 2019-05-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 436 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.50 Å |
| 6OST RF2 pre-accommodated state bound Release complex 70S at 24ms Deposited 2019-05-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 12 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.20 Å |
| 6OT3 RF2 accommodated state bound Release complex 70S at 24 ms Deposited 2019-05-02 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 12 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.90 Å |
| 6OUO RF2 accommodated state bound 70S complex at long incubation time Deposited 2019-05-05 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 12 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.70 Å |
| 6PJ6 High resolution cryo-EM structure of E.coli 50S Deposited 2019-06-27 | Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric（31） Consistent with all polymers |
Chain k
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 185 NA SODIUM ION × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；Buffer A
cryo-EM vitrification conditions
Cryogen ETHANE；Blot Force 5
Blot Time 10sec
Hum 95%
Temperature 10C
Waiting time 1 min
|
Resolution 2.20 Å |
| 6U48 E. coli 50S with phazolicin (PHZ) bound in exit tunnel Deposited 2019-08-23 | Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 32-meric（32） Consistent with all polymers |
Chain C3
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.87 Å |
| 6VU3 Cryo-EM structure of Escherichia coli transcription-translation complex A (TTC-A) containing mRNA with a 12 nt long spacer Deposited 2020-02-14 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6VYQ Escherichia coli transcription-translation complex A1 (TTC-A1) containing an 15 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6VYR Escherichia coli transcription-translation complex A1 (TTC-A1) containing an 18 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6VYS Escherichia coli transcription-translation complex A1 (TTC-A1) containing a 21 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6VYT Escherichia coli transcription-translation complex A2 (TTC-A2) containing a 15 nt long mRNA spacer, NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 55 PDB declaration: 64-meric（64） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 14.00 Å |
| 6VYU Escherichia coli transcription-translation complex C2 (TTC-C2) containing a 27 nt long mRNA spacer Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.00 Å |
| 6VYW Escherichia coli transcription-translation complex C3 (TTC-C3) containing mRNA with a 27 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.00 Å |
| 6VYX Escherichia coli transcription-translation complex C4 (TTC-C4) containing mRNA with a 21 nt long spacer, transcription factor NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.90 Å |
| 6VYY Escherichia coli transcription-translation complex C5 (TTC-C5) containing mRNA with a 21 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.90 Å |
| 6VYZ Escherichia coli transcription-translation complex C6 (TTC-C6) containing mRNA with a 21 nt long spacer, NusA, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.90 Å |
| 6VZ2 Escherichia coli transcription-translation complex D1 (TTC-D1) containing mRNA with a 27 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 10.00 Å |
| 6VZ3 Escherichia coli transcription-translation complex D2 (TTC-D2) containing mRNA with a 27 nt long spacer Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.90 Å |
| 6VZ5 Escherichia coli transcription-translation complex D3 (TTC-D3) containing mRNA with a 21 nt long spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.90 Å |
| 6VZ7 Escherichia coli transcription-translation complex C1 (TTC-C1) containing a 27 nt long mRNA spacer, NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-02-27 | Assembly 1 Other combination Heteromer；Protein × 56 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.00 Å |
| 6VZJ Escherichia coli transcription-translation complex A1 (TTC-A1) containing mRNA with a 15 nt long spacer, fMet-tRNAs at E-site and P-site, and lacking transcription factor NusG Deposited 2020-02-28 | Assembly 1 Other combination Heteromer；Protein × 57 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 6WD0 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure I-A) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain D
1–46（46 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 6WD1 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure I-B) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain D
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 6WD2 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-A) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D
1–46（46 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6WD3 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-B1) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D
1–46（46 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6WD4 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-B2) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D
1–46（46 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6WD5 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-C1) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D
1–46（46 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6WD7 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-D) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D
1–46（46 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6WD8 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure III-A) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D
1–46（46 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6WD9 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure III-B) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D
1–46（46 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6WDA Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure III-C) Deposited 2020-03-31 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain D
1–46（46 aa）
|
Not recorded | FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6X6T Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B1 (TTC-B1) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-05-29 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6X7F Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B2 (TTC-B2) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-05-29 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6X7K Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-05-30 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6X9Q Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 27 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-06-03 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å |
| 6XDQ Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 30 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site Deposited 2020-06-11 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6XDR Escherichia coli transcription-translation complex B (TTC-B) containing an 27 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-11 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.70 Å |
| 6XGF Escherichia coli transcription-translation complex B (TTC-B) containing an 30 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-17 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.00 Å |
| 6XII Escherichia coli transcription-translation complex B (TTC-B) containing an 24 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-20 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.00 Å |
| 6XIJ Escherichia coli transcription-translation complex A (TTC-A) containing an 24 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site Deposited 2020-06-20 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.00 Å |
| 7K00 Structure of the Bacterial Ribosome at 2 Angstrom Resolution Deposited 2020-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 1
1–46（46 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 309 SPD SPERMIDINE × 16 SPM SPERMINE × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.98 Å |
| 7LVK Cfr-modified 50S subunit from Escherichia coli Deposited 2021-02-25 | Assembly 1 Protein–RNA Heteromer；Protein × 28 PDB declaration: 30-meric（30） Consistent with all polymers |
Chain k
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 179 NA SODIUM ION × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；Buffer A
cryo-EM vitrification conditions
Cryogen ETHANE；Blot Force 5
Blot Time 10sec
Hum 95%
Temperature 10C
Waiting time 1 min
|
Resolution 2.20 Å |
| 7M5D Cryo-EM structure of a non-rotated E.coli 70S ribosome in complex with RF3-GTP, RF1 and P-tRNA (state I) Deposited 2021-03-23 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 7S1G wild-type Escherichia coli stalled ribosome with antibiotic linezolid Deposited 2021-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain k
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 196 ZLD N-{[(5S)-3-(3-fluoro-4-morpholin-4-ylphenyl)-2-oxo-1,3-oxazolidin-5-yl]methyl}acetamide × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.48 Å |
| 7S1H Wild-type Escherichia coli ribosome with antibiotic linezolid Deposited 2021-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain k
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 206 ZLD N-{[(5S)-3-(3-fluoro-4-morpholin-4-ylphenyl)-2-oxo-1,3-oxazolidin-5-yl]methyl}acetamide × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.35 Å |
| 7S1I Wild-type Escherichia coli stalled ribosome with antibiotic radezolid Deposited 2021-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain k
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 209 RD8 Radezolid × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.48 Å |
| 7S1J Wild-type Escherichia coli ribosome with antibiotic radezolid Deposited 2021-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain k
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 199 RD8 Radezolid × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.47 Å |
| 7S1K Cfr-modified Escherichia coli stalled ribosome with antibiotic radezolid Deposited 2021-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain k
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 207 RD8 Radezolid × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.42 Å |
| 7SA4 Damaged 70S ribosome with PrfH bound Deposited 2021-09-22 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 439 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.55 Å |
| 7TOS E. coli 70S ribosomes bound with the ALS/FTD-associated dipeptide repeat protein PR20 Deposited 2022-01-24 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain L34
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7UG7 70S ribosome complex in an intermediate state of translocation bound to EF-G(GDP) stalled by Argyrin B Deposited 2022-03-24 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain Lh
1–46（46 aa）
|
Not recorded | PUT 1,4-DIAMINOBUTANE × 11 MG MAGNESIUM ION × 404 SPD SPERMIDINE × 2 ZN ZINC ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 FME N-FORMYLMETHIONINE × 1 PHE PHENYLALANINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.58 Å |
| 7UPH Structure of a ribosome with tethered subunits Deposited 2022-04-15 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 49-meric（49） Consistent with all polymers |
Chain k
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.18 Å |
| 8E30 E. coli 50S ribosome bound to compound streptogramin A analog 3142 Deposited 2022-08-16 | Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain R
1–46（46 aa）
|
Not recorded | UE6 N-{2-[4-(aminomethyl)-1H-1,2,3-triazol-1-yl]ethyl}-2-[(3R,4R,5E,10E,12E,14S,16R,23S,26aR)-16-fluoro-14-hydroxy-12-methyl-1,7,22-trioxo-3-(propan-2-yl)-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-4-yl]acetamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.91 Å |
| 8E32 E. coli 50S ribosome bound to compound streptogramin analogs SA1 and SB1 Deposited 2022-08-16 | Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain R
1–46（46 aa）
|
Not recorded | UCX (3R,4R,5E,10E,12E,14S,16R,23S,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-yn-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione × 1 UDF ~{N}-[(3~{S},6~{S},12~{R},15~{S},16~{R},19~{S},22~{S},24~{R})-12-[2-[(azanylidene-$l^{4}-azanylidene)amino]ethyl]-4,16-dimethyl-24-oxidanyl-2,5,11,14,18,21-hexakis(oxidanylidene)-19-phenyl-3-(phenylmethyl)-17-oxa-1,4,10,13,20-pentazatricyclo[20.4.0.0^{6,10}]hexacosan-15-yl]-3-oxidanyl-pyridine-2-carboxamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.35 Å |
| 8E33 E. coli 50S ribosome bound to compound streptogramin analog SAB001 Deposited 2022-08-16 | Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain R
1–46（46 aa）
|
Not recorded | UDU N-{(4R,6R,9S,10R,13S,15aS,17R,20R,22S,24aS)-22-benzyl-6-[2-(4-{[(3R,4R,5E,10E,12E,14S,16R,23S,26aR)-16-fluoro-14-hydroxy-12-methyl-1,7,22-trioxo-3-(propan-2-yl)-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-4-yl]methyl}-1H-1,2,3-triazol-1-yl)ethyl]-17-hydroxy-10,23-dimethyl-5,8,12,15,21,24-hexaoxo-13-phenyldocosahydro-12H-pyrido[2,1-f]pyrrolo[2,1-l][1,4,7,10,13,16]oxapentaazacyclononadecin-9-yl}-3-hydroxypyridine-2-carboxamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.23 Å |
| 8E35 E. coli 50S ribosome bound to compound SAB002 Deposited 2022-08-16 | Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain R
1–46（46 aa）
|
Not recorded | UCR N-{(4R,6R,9S,10R,13S,15aS,17R,20R,22S,24aS)-22-benzyl-6-[3-(4-{[(3R,4R,5E,10E,12E,14S,16R,23R,26aR)-16-fluoro-14-hydroxy-12-methyl-1,7,22-trioxo-3-(propan-2-yl)-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-4-yl]methyl}-1H-1,2,3-triazol-1-yl)propyl]-17-hydroxy-10,23-dimethyl-5,8,12,15,21,24-hexaoxo-13-phenyldocosahydro-12H-pyrido[2,1-f]pyrrolo[2,1-l][1,4,7,10,13,16]oxapentaazacyclononadecin-9-yl}-3-hydroxypyridine-2-carboxamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.27 Å |
| 8E36 E. coli 50S ribosome bound to compound streptogramin A analog 3146 Deposited 2022-08-16 | Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain R
1–46（46 aa）
|
Not recorded | UEC 2-[(3R,4R,5E,10E,12E,14S,16R,23S,26aR)-16-fluoro-14-hydroxy-12-methyl-1,7,22-trioxo-3-(propan-2-yl)-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-4-yl]-N-{2-[4-(pyridin-4-yl)-1H-1,2,3-triazol-1-yl]ethyl}acetamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.38 Å |
| 8E3L E. coli 50S ribosome bound to D-linker solithromycin conjugate Deposited 2022-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain R
1–46（46 aa）
|
Not recorded | UK3 (2~{R})-~{N}-[(2~{R})-6-azanyl-1-[[(2~{R})-1-[[(2~{R})-1-[[3-[1-[4-[(1~{S},2~{R},5~{S},7~{R},8~{R},9~{R},11~{R},13~{R},14~{R})-8-[(2~{S},3~{R},4~{S},6~{R})-4-(dimethylamino)-6-methyl-3-oxidanyl-oxan-2-yl]oxy-2-ethyl-5-fluoranyl-9-methoxy-1,5,7,11,13-pentamethyl-4,6,12,16-tetrakis(oxidanylidene)-3,17-dioxa-15-azabicyclo[12.3.0]heptadecan-15-yl]butyl]-1,2,3-triazol-4-yl]phenyl]amino]-4-methylsulfanyl-1-oxidanylidene-butan-2-yl]amino]-3-(4-hydroxyphenyl)-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-hexan-2-yl]-1-[(2~{R})-2-[[(2~{R})-2-[2-[[2,3-bis(oxidanyl)phenyl]carbonyl-[4-[[2,3-bis(oxidanyl)phenyl]carbonylamino]butyl]amino]ethanoylamino]-3-(1~{H}-indol-3-yl)propanoyl]amino]-3-oxidanyl-propanoyl]pyrrolidine-2-carboxamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.35 Å |
| 8E3M E. coli 50S ribosome bound to L-linker solithromycin conjugate Deposited 2022-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain R
1–46（46 aa）
|
Not recorded | UIU N-acetyl-L-tyrosyl-N-[(3M)-3-(1-{4-[(3aS,4R,7S,9R,10R,11R,13R,15R,15aR)-4-ethyl-7-fluoro-11-methoxy-3a,7,9,11,13,15-hexamethyl-2,6,8,14-tetraoxo-10-{[3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranosyl]oxy}dodecahydro-2H-oxacyclotetradecino[4,3-d][1,3]oxazol-1(4H)-yl]butyl}-1H-1,2,3-triazol-4-yl)phenyl]-L-methioninamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.25 Å |
| 8E3O E. coli 50S ribosome bound to solithromycin and VM1 Deposited 2022-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain R
1–46（46 aa）
|
Not recorded | EM1 (3aS,4R,7S,9R,10R,11R,13R,15R,15aR)-1-{4-[4-(3-aminophenyl)-1H-1,2,3-triazol-1-yl]butyl}-4-ethyl-7-fluoro-11-methoxy-3a ,7,9,11,13,15-hexamethyl-2,6,8,14-tetraoxotetradecahydro-2H-oxacyclotetradecino[4,3-d][1,3]oxazol-10-yl 3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranoside × 1 VIR VIRGINIAMYCIN M1 × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.99 Å |
| 8E41 E. coli 50S ribosome bound to tiamulin and VS1 Deposited 2022-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: undecameric（11） Consistent with all polymers |
Chain R
1–46（46 aa）
|
Not recorded | MUL TIAMULIN × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.13 Å |
| 8E42 E. coli 50S ribosome bound to tiamulin and azithromycin Deposited 2022-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain R
1–46（46 aa）
|
Not recorded | MUL TIAMULIN × 1 ZIT AZITHROMYCIN × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.29 Å |
| 8E43 E. coli 50S ribosome bound to compound streptogramin A analog 3336 Deposited 2022-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain R
1–46（46 aa）
|
Not recorded | UI0 (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,23S,26aR)-16-fluoro-14-hydroxy-12-methyl-1,7,22-trioxo-4-(prop-2-en-1-yl)-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.09 Å |
| 8E44 E. coli 50S ribosome bound to antibiotic analog SLC09 Deposited 2022-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain R
1–46（46 aa）
|
Not recorded | UI9 2-chloro-N-{[(5S)-3-{(4M)-3-fluoro-4-[(6P)-6-(2-methyl-2H-tetrazol-5-yl)pyridin-3-yl]phenyl}-2-oxo-1,3-oxazolidin-5-yl]methyl}acetamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.53 Å |
| 8E45 E. coli 50S ribosome bound to antibiotic analog SLC17 Deposited 2022-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain R
1–46（46 aa）
|
Not recorded | UI6 2-{[(2S,3R,4S,6R)-2-{[(2R,3S,4R,5R,8R,10R,11R,12S,13S,14R)-2-ethyl-3,4,10-trihydroxy-13-{[(2R,4R,5S,6S)-5-hydroxy-4-methoxy-4,6-dimethyloxan-2-yl]oxy}-3,5,6,8,10,12,14-heptamethyl-15-oxo-1-oxa-6-azacyclopentadecan-11-yl]oxy}-3-hydroxy-6-methyloxan-4-yl](methyl)amino}-N-{[(5S)-3-{(4M)-3-fluoro-4-[(6P)-6-(2-methyl-2H-tetrazol-5-yl)pyridin-3-yl]phenyl}-2-oxo-1,3-oxazolidin-5-yl]methyl}acetamide (non-preferred name) × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.30 Å |
| 8E46 E. coli 50S ribosome bound to antibiotic analog SLC21 Deposited 2022-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain R
1–46（46 aa）
|
Not recorded | UHP (2R,3S,4R,5R,8R,10R,11R,12S,13S,14R)-2-ethyl-3,4,10-trihydroxy-3,5,6,8,10,12,14-heptamethyl-15-oxo-11-({3,4,6-trideoxy-3-[{[1-(2-{[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]amino}-2-oxoethyl)-1H-1,2,3-triazol-4-yl]methyl}(methyl)amino]-beta-D-xylo-hexopyranosyl}oxy)-1-oxa-6-azacyclopentadecan-13-yl 2,6-dideoxy-3-C-methyl-3-O-methyl-alpha-L-ribo-hexopyranoside × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.32 Å |
| 8E47 E. coli 50S ribosome bound to antibiotic analog SLC26 Deposited 2022-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain R
1–46（46 aa）
|
Not recorded | UH6 (2R,3S,4R,5R,8R,10R,11R,12S,13S,14R)-2-ethyl-3,4,10-trihydroxy-3,5,6,8,10,12,14-heptamethyl-15-oxo-11-({3,4,6-trideoxy-3-[(2-{[2-({[(5S)-3-{(4M)-3-fluoro-4-[(6P)-6-(2-methyl-2H-tetrazol-5-yl)pyridin-3-yl]phenyl}-2-oxo-1,3-oxazolidin-5-yl]methyl}amino)-2-oxoethyl]sulfanyl}ethyl)(methyl)amino]-beta-D-xylo-hexopyranosyl}oxy)-1-oxa-6-azacyclopentadecan-13-yl 2,6-dideoxy-3-C-methyl-3-O-methyl-alpha-L-ribo-hexopyranoside × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.32 Å |
| 8E48 E. coli 50S ribosome bound to antibiotic analog SLC30 Deposited 2022-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain R
1–46（46 aa）
|
Not recorded | UIF [(2~{S},3~{S},4~{R},6~{R})-4-[[2-[[(5~{S})-3-[3-fluoranyl-4-[6-(2-methyl-1,2,3,4-tetrazol-5-yl)pyridin-3-yl]phenyl]-2-oxidanylidene-1,3-oxazolidin-5-yl]methylamino]-2-oxidanylidene-ethyl]-methyl-amino]-6-methyl-2-(phenylcarbonyloxy)oxan-3-yl] benzoate × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.27 Å |
| 8E49 E. coli 50S ribosome bound to antibiotic analog SLC31 Deposited 2022-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain R
1–46（46 aa）
|
Not recorded | UH0 ~{N}-[[(5~{S})-3-[3-fluoranyl-4-[6-(2-methyl-1,2,3,4-tetrazol-5-yl)pyridin-3-yl]phenyl]-2-oxidanylidene-1,3-oxazolidin-5-yl]methyl]-2-[methyl-[(2~{R},3~{R},4~{S},6~{R})-6-methyl-2,3-bis(oxidanyl)oxan-4-yl]amino]ethanamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.05 Å |
| 8EIU E. coli 70S ribosome with A-loop mutations U2554C and U2555C Deposited 2022-09-15 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 1
1–46（46 aa）
|
Not recorded | ZN ZINC ION × 2 PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 268 SPD SPERMIDINE × 16 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 SPM SPERMINE × 1 MET METHIONINE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.24 Å |
| 8EKC Escherichia coli 70S ribosome bound to thermorubin, deacylated P-site tRNAfMet and aminoacylated A-site Phe-tRNA Deposited 2022-09-20 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 6
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 714 T8B Thermorubin × 1 PHE PHENYLALANINE × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8EMM Composite 70S ribosome structure for "Atomistic simulations of the E. coli ribosome provide selection criteria for translationally active substrates Deposited 2022-09-28 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 1
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 321 SPD SPERMIDINE × 17 SPM SPERMINE × 1 K POTASSIUM ION × 10 ZN ZINC ION × 2 MET METHIONINE × 2 PAR PAROMOMYCIN × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.10 Å |
| 8FIZ Cryo-EM structure of E. coli 70S Ribosome containing mRNA and tRNA (in the transcription-translation complex) Deposited 2022-12-18 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain BN
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8FTO E. coli 70S ribosome with an improved MS2 tag inserted in H98 Deposited 2023-01-12 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 1
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 284 FME N-FORMYLMETHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.85 Å |
| 8FZD Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with apoRF3, RF1, P- and E-site tRNAPhe (Composite state I-B) Deposited 2023-01-28 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain 6
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 581 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8FZE Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with RF1, P- and E-site tRNAPhe (State I-A) Deposited 2023-01-28 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain 6
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 570 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8FZF Cryo-EM structure of an E. coli rotated ribosome complex bound with RF3-ppGpp and p/E-tRNAPhe (Composite state I-C) Deposited 2023-01-28 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 6
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 384 G4P GUANOSINE-5',3'-TETRAPHOSPHATE × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8FZG Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with RF3-GDPCP, RF1, P- and E-site tRNAPhe (Composite state II-A) Deposited 2023-01-28 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain 6
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 428 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8FZH Cryo-EM structure of an E. coli non-rotated ribosome termination complex bound with RF1, P- and E-site tRNAPhe (State II-D) Deposited 2023-01-28 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain 6
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 595 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8FZI Cryo-EM structure of an E. coli rotated ribosome bound with RF3-GDPCP and p/E-tRNAPhe (Composite state II-B) Deposited 2023-01-28 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 6
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 737 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8FZJ Cryo-EM structure of an E. coli rotated ribosome bound with RF3-GDPCP and p/E-tRNAPhe (Composite state II-C) Deposited 2023-01-28 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 6
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 757 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；5 mM Tris-HCl, 60 mM NH4Cl, 10 mM MgCl2, 6 mM B-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8G2U Time-resolved cryo-EM study of the 70S recycling by the HflX:control-apo-70S at 900ms Deposited 2023-02-06 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain 2
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8G31 Time-resolved cryo-EM study of the 70S recycling by the HflX:2nd Intermediate Deposited 2023-02-06 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain 2
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8G34 Time-resolved cryo-EM study of the 70S recycling by the HflX:1st intermediate Deposited 2023-02-06 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain 2
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8G38 Time-resolved cryo-EM study of the 70S recycling by the HflX:3rd Intermediate Deposited 2023-02-07 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain 2
1–46（46 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8G6W Structure of WT E.coli 70S ribosome complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site ortho-aminobenzoic acid charged NH-tRNAPhe Deposited 2023-02-16 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 1
1–46（46 aa）
|
Not recorded | ZN ZINC ION × 2 PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 320 2AE 2-AMINO-BENZAMIDE × 1 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 1 FME N-FORMYLMETHIONINE × 1 K POTASSIUM ION × 14 SPD SPERMIDINE × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.02 Å |
| 8G6X Structure of WT E.coli ribosome 50S subunit with complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site meta-aminobenzoic acid charged NH-tRNAPhe Deposited 2023-02-16 | Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 34-meric（34） Consistent with all polymers |
Chain 1
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 223 SPD SPERMIDINE × 17 SPM SPERMINE × 1 K POTASSIUM ION × 7 FME N-FORMYLMETHIONINE × 1 ZN ZINC ION × 2 3AB 3-aminobenzamide × 1 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.31 Å |
| 8G6Y Structure of WT E.coli ribosome 50S subunit with complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site 3-aminopyridine-4-carboxylic acid charged NH-tRNAPhe Deposited 2023-02-16 | Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 34-meric（34） Consistent with all polymers |
Chain 1
1–46（46 aa）
|
Not recorded | ZN ZINC ION × 2 YRW 3-aminopyridine-4-carboxamide × 1 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 1 FME N-FORMYLMETHIONINE × 1 MG MAGNESIUM ION × 231 K POTASSIUM ION × 15 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.09 Å |
| 8G7P Structure of the Escherichia coli 70S ribosome in complex with EF-Tu and Ile-tRNAIle(LAU) bound to the cognate AUA codon (Structure I) Deposited 2023-02-16 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain 6
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 876 K POTASSIUM ION × 2 ILE ISOLEUCINE × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8G7Q Structure of the Escherichia coli 70S ribosome in complex with EF-Tu and Ile-tRNAIle(LAU) bound to the near-cognate AUG codon (Structure II) Deposited 2023-02-16 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain 6
1–46（46 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 950 ILE ISOLEUCINE × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8G7R Structure of the Escherichia coli 70S ribosome in complex with A-site tRNAIle(LAU) bound to the cognate AUA codon (Structure III) Deposited 2023-02-16 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 6
1–46（46 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 734 K POTASSIUM ION × 2 ILE ISOLEUCINE × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 8G7S Structure of the Escherichia coli 70S ribosome in complex with P-site tRNAIle(LAU) bound to the cognate AUA codon (Structure IV) Deposited 2023-02-16 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 6
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 564 ILE ISOLEUCINE × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8SYL Cryo-EM structure of the Escherichia coli 70S ribosome in complex with amikacin, mRNA, and A-, P-, and E-site tRNAs Deposited 2023-05-25 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 6
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 474 AKN (2S)-N-[(1R,2S,3S,4R,5S)-4-[(2R,3R,4S,5S,6R)-6-(aminomethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-5-azanyl-2-[(2S,3R,4S,5S ,6R)-4-azanyl-6-(hydroxymethyl)-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-cyclohexyl]-4-azanyl-2-oxidanyl-butanamide × 2 PHE PHENYLALANINE × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8T5D Cryo-EM studies of the interplay between uS2 ribosomal protein and leaderless mRNA during bacterial translation initiation Deposited 2023-06-13 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 2
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8T5H Cryo-EM studies of the interplay between uS2 ribosomal protein and leaderless mRNA during bacterial translation initiation Deposited 2023-06-13 | Assembly 1 Protein–RNA Heteromer；Protein × 46 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain 2
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8UPO Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-23 | Assembly 1 Other combination Heteromer；Protein × 58 PDB declaration: 66-meric（66） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.50 Å |
| 8UPR Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-23 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.30 Å |
| 8UQL Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH in loaded state, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-24 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8UQM Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH in loaded state, NusA, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-24 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.30 Å |
| 8UQP Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-24 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8UR0 Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 24 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-25 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8URH Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 27 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-26 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.70 Å |
| 8URI Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 27 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-26 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.30 Å |
| 8URM E. coli 70S ribosome with unmodified tRNAPro(GGG) bound to slippery P-site CCC-C codon and tRNAVal(UAC) in the A site Deposited 2023-10-26 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 395 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8URX Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 30 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome Deposited 2023-10-27 | Assembly 1 Other combination Heteromer；Protein × 59 PDB declaration: 67-meric（67） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å |
| 8URY Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 30 nt long spacer, and fMet-tRNA in E-site and P-site of the ribosome Deposited 2023-10-27 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8UTJ E. coli 70S ribosome with unmodified lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the 0 frame Deposited 2023-10-31 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 530 LYS LYSINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8UX8 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the +1 mRNA reading frame Deposited 2023-11-09 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 395 LYS LYSINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8UXB E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-09 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 287 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8UZ3 E. coli 70S ribosome with unmodified e*/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-14 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 415 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8UZG E. coli 70S ribosome with unmodified e*/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-15 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 398 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 8V03 E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon Deposited 2023-11-16 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 281 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 8VKV Escherichia coli transcription-translation coupled complex (TTC-B) containing mRNA with a 33 nt long spacer, ops signal, RfaH, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-10 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8VL1 Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 36 nt long spacer, ops signal, RfaH, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-11 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.30 Å |
| 8VOO Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 39 nt long spacer, ops signal, RfaH, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8VOP Escherichia coli transcription-translation coupled complex (TTC-B) containing mRNA with a 36 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.00 Å |
| 8VOQ Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 39 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.30 Å |
| 8VOR Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 51 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8VOS Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 60 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2024-01-15 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å |
| 8VS9 Endogenous trans-translation complex with tmRNA*SmpB in the P site and alanyl-tRNA in the A site and deacyl-tRNA in the E site of E. coli 70S ribosome Deposited 2024-01-23 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain L34
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 8VSA Endogenous trans-translation complex with tmRNA*SmpB in the P site and alanyl-tRNA in the A site of E. coli 70S ribosome Deposited 2024-01-23 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain L34
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 8VUP E. coli 70S ribosome with unmodified tRNAPro(GGG) in the e*/E conformation on a slippery CCC-C codon Deposited 2024-01-29 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 239 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8VV4 E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon Deposited 2024-01-30 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 424 LYS LYSINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8VWQ E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon and Elongation Factor P bound (uL1 in the closed conformation) Deposited 2024-02-02 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 586 LYS LYSINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8W2N E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon and Elongation Factor P bound (uL1 in the open conformation) Deposited 2024-02-20 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 481 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9AX7 70S initiation complex (tRNA-fMet M1 + CUG start codon) Deposited 2024-03-06 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 1
1–46（46 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.63 Å |
| 9AX8 70S initiation complex (tRNA-fMet M1, initiation factor 2 + CUG start codon) Deposited 2024-03-06 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 7
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 287 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9B4Z E. coli 70S ribosome complex (N1-methylated 16S A1408 + arbekacin) Deposited 2024-03-21 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain B2
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 309 84G Arbekacin × 2 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å |
| 9B50 E. coli 70S ribosome complex (unmethylated 16S A1408 + arbekacin) Deposited 2024-03-21 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain B2
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 309 84G Arbekacin × 2 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9B51 E. coli 70S ribosome complex (N1-methylated 16S A1408 + G418) Deposited 2024-03-21 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain B2
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 309 GET GENETICIN × 2 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 9CG5 70S initiation complex (tRNA-fMet M1 + UUG start codon) Deposited 2024-06-28 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 1
1–46（46 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.59 Å |
| 9CG6 70S initiation complex (tRNA-fMet M1 + GUG start codon) Deposited 2024-06-28 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 1
1–46（46 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.61 Å |
| 9CG7 70S initiation complex (tRNA-fMet M1 + AUG start codon) Deposited 2024-06-28 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 1
1–46（46 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 309 IAS BETA-L-ASPARTIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.75 Å |
| 9D89 E. coli 50S ribosomal subunit in complex with PrAMP rumicidin-2 (focused refinement) Deposited 2024-08-19 | Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric（31） Consistent with all polymers |
Chain 1
1–46（46 aa）
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 204 K POTASSIUM ION × 93 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.95 Å |
| 9MKK Structure of arbekacin bound Escherichia coli 70S ribosome Deposited 2024-12-17 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain 1
1–46（46 aa）
|
Not recorded | 84G Arbekacin × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9MOR Damaged 70S ribosome with PrfH bound Deposited 2024-12-27 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 439 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.65 Å |
| 9MQ4 Damaged 70S ribosome with PrfH bound Deposited 2025-01-01 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain d
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 439 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.78 Å |
| 9NJF E. coli pre-elongation complex without an A-site tRNA with EQ2-YbiT in Non-hydrolytic 1/PtIM(a) conformation Deposited 2025-02-27 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain 34
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 124 ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9NJV E. coli 70S initiation complex (bL33 absent) Deposited 2025-02-28 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain 34
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 152 FME N-FORMYLMETHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9NKL E. coli 70S initiation complex Deposited 2025-03-01 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 34
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 161 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9NL5 E. coli pre-elongation complex without an A-site tRNA with EQ2-EttA in Hydrolytic 1 conformation Deposited 2025-03-02 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 34
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 125 ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9NL6 E. coli initiation complex with EQ2-YbiT in Non-hydrolytic 1/PtIM(a) conformation Deposited 2025-03-02 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 34
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 252 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9NL7 E. coli initiation complex with EQ2-YbiT in Non-hydrolytic 2/PtIM(b) conformation Deposited 2025-03-02 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 34
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 250 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9NLB E. coli initiation complex with EQ2-EttA in Hydrolytic 2 conformation Deposited 2025-03-03 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 34
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 279 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 FME N-FORMYLMETHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9NLE E. coli initiation complex with EQ2-EttA in Hydrolytic 1 conformation Deposited 2025-03-03 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 34
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 298 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 FME N-FORMYLMETHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9NLF E. coli initiation complex with EQ2-YbiT in Hydrolytic 2/PtIM(a) conformation Deposited 2025-03-03 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 34
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 245 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9NLH E.coli Initiation complex with YheS-EQ2 Deposited 2025-03-03 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 34
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 263 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9NLJ E.coli Initiation complex with Uup-EQ2 Deposited 2025-03-03 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 34
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 277 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9NLQ E. coli initiation complex with EQ2-YbiT in Hydrolytic 1/PtIM(a) conformation Deposited 2025-03-03 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 34
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 241 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9NLS E. coli initiation complex with EQ2-YbiT in Intermediate/PtIM(b) conformation Deposited 2025-03-03 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 34
1–46（46 aa）
|
Not recorded | MG MAGNESIUM ION × 248 ZN ZINC ION × 1 FME N-FORMYLMETHIONINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 NA SODIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9O2X Structure of WT E.coli ribosome 70S subunit with complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site (S)-betahydroxyBocK charged NH-tRNAPyl Deposited 2025-04-04 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 1
1–46（46 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 320 FME N-FORMYLMETHIONINE × 1 K POTASSIUM ION × 10 PAR PAROMOMYCIN × 1 SPD SPERMIDINE × 14 SPM SPERMINE × 1 A1B71 (2S)-6-[(tert-butoxycarbonyl)amino]-2-(hydroxymethyl)hexanoic acid × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.29 Å |
| 9O2Y Structure of WT E.coli ribosome 70S subunit with complexed with mRNA, P-site fMet-NH-tRNAfMet and A-site (R) beta-2-hydroxy-BocLysine acid charged NH-tRNAPyl Deposited 2025-04-04 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 1
1–46（46 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 320 SPD SPERMIDINE × 13 FME N-FORMYLMETHIONINE × 1 SPM SPERMINE × 1 K POTASSIUM ION × 10 ZN ZINC ION × 2 A1B70 (2R)-6-[(tert-butoxycarbonyl)amino]-2-(hydroxymethyl)hexanoic acid × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.14 Å |
| 9PIH E. coli 70S ribosome bound to Doxycycline Deposited 2025-07-10 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 1
1–46（46 aa）
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 276 K POTASSIUM ION × 92 DXT (4S,4AR,5S,5AR,6R,12AS)-4-(DIMETHYLAMINO)-3,5,10,12,12A-PENTAHYDROXY-6-METHYL-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 9PII E. coli 70S ribosome bound to Sarecycline Deposited 2025-07-10 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 1
1–46（46 aa）
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 281 K POTASSIUM ION × 100 V7A Sarecycline × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 9PIJ E. coli 70S ribosome bound to Minocycline Deposited 2025-07-10 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 1
1–46（46 aa）
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 340 K POTASSIUM ION × 148 MIY (4S,4AS,5AR,12AS)-4,7-BIS(DIMETHYLAMINO)-3,10,12,12A-TETRAHYDROXY-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2- CARBOXAMIDE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å |
| 9Y79 Escherichia coli transcription-translation loosely coupled complex (TTC-LC^walked) containing mRNA with a 39 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site Deposited 2025-09-09 | Assembly 1 Other combination Heteromer；Protein × 60 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain m
1–46（46 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 3.20 Å |