Current Protein Identity：P52655
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1NVP HUMAN TFIIA/TBP/DNA COMPLEX Deposited 2003-02-04 | Assembly 1 Protein–DNA Heteromer；Protein × 4 PDB declaration: hexameric（6） Consistent with all polymers |
Chain B
2–58（57 aa）
Fragment：N-terminal 58 amino acids
Chain C
303–376（74 aa）
Fragment：c-terminal 76 amino acids
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；PEG6000, lithium nitrate, calcium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.10 Å R-free 0.247 |
| 5FUR Structure of human TFIID-IIA bound to core promoter DNA Deposited 2016-01-29 | Assembly 1 Protein–DNA Heteromer；Protein × 10 PDB declaration: dodecameric（12） Consistent with all polymers |
Chain B
9–51（43 aa）
Chain C
330–376（47 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
10 MM HEPES, 10 MM MGCL2, 50 MM KCL, 3% TREHALOSE 1 MM DTT, 0.0125% NP-40；pH 7.9；10 MM HEPES, 10 MM MGCL2, 50 MM KCL, 3% TREHALOSE 1 MM DTT, 0.0125% NP-40
cryo-EM vitrification conditions
Cryogen ETHANE；LIQUID ETHANE
|
Resolution 8.50 Å |
| 5IY6 Human holo-PIC in the closed state Deposited 2016-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 27 PDB declaration: 29-meric（29） Consistent with all polymers |
Chain N
1–376（376 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 11 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 7.20 Å |
| 5IY7 Human holo-PIC in the open state Deposited 2016-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 27 PDB declaration: 29-meric（29） Consistent with all polymers |
Chain N
1–376（376 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 11 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 8.60 Å |
| 5IY8 Human holo-PIC in the initial transcribing state Deposited 2016-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 27 PDB declaration: 29-meric（29） Consistent with all polymers |
Chain N
1–376（376 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 11 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 7.90 Å |
| 5IY9 Human holo-PIC in the initial transcribing state (no IIS) Deposited 2016-03-24 | Assembly 1 Other combination Heteromer；Protein × 26 PDB declaration: 29-meric（29） Consistent with all polymers |
Chain N
1–376（376 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 10 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 6.30 Å |
| 5IYA Human core-PIC in the closed state Deposited 2016-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 21 PDB declaration: 23-meric（23） Consistent with all polymers |
Chain N
1–376（376 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 11 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 5.40 Å |
| 5IYB Human core-PIC in the open state Deposited 2016-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 21 PDB declaration: 23-meric（23） Consistent with all polymers |
Chain N
1–376（376 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 11 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.90 Å |
| 5IYC Human core-PIC in the initial transcribing state Deposited 2016-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 21 PDB declaration: 23-meric（23） Consistent with all polymers |
Chain N
1–376（376 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 11 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.90 Å |
| 5IYD Human core-PIC in the initial transcribing state (no IIS) Deposited 2016-03-24 | Assembly 1 Other combination Heteromer；Protein × 20 PDB declaration: 23-meric（23） Consistent with all polymers |
Chain N
1–376（376 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 11 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.90 Å |
| 5M4S Transcription factor TFIIA as a single chain protein Deposited 2016-10-19 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
2–58（57 aa）
Chain A
328–376（49 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；20mM Tris (8.0), 1mM DTT, 0.5mM EDTA and 25mM NaCl
|
Resolution 2.38 Å R-free 0.242 |
| 6O9L Human holo-PIC in the closed state Deposited 2019-03-14 | Assembly 1 Protein–DNA Heteromer；Protein × 31 PDB declaration: 33-meric（33） Consistent with all polymers |
Chain N
1–376（376 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.20 Å |
| 7EDX p53-bound TFIID-based core PIC on HDM2 promoter Deposited 2021-03-17 | Assembly 1 Protein–DNA Heteromer；Protein × 37 PDB declaration: 39-meric（39） Consistent with all polymers |
Chain Q
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 7EG7 TFIID-based core PIC on SCP promoter Deposited 2021-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 37 PDB declaration: 39-meric（39） Consistent with all polymers |
Chain Q
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.20 Å |
| 7EG8 TFIID-based core PIC on PUMA promoter Deposited 2021-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 37 PDB declaration: 39-meric（39） Consistent with all polymers |
Chain Q
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.40 Å |
| 7EG9 TFIID-based intermediate PIC on SCP promoter Deposited 2021-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 39 PDB declaration: 41-meric（41） Consistent with all polymers |
Chain Q
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 10 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 7EGA TFIID-based intermediate PIC on PUMA promoter Deposited 2021-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 39 PDB declaration: 41-meric（41） Consistent with all polymers |
Chain Q
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 10 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 7EGB TFIID-based holo PIC on SCP promoter Deposited 2021-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 49 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain Q
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 7EGC p53-bound TFIID-based holo PIC on HDM2 promoter Deposited 2021-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 49 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain Q
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 16 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 7EGD SCP promoter-bound TFIID-TFIIA in initial TBP-loading state Deposited 2021-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 22 PDB declaration: 24-meric（24） Consistent with all polymers |
Chain Q
1–376（376 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.75 Å |
| 7EGI TFIID in rearranged conformation Deposited 2021-03-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric（22） Consistent with protein count |
Chain Q
1–376（376 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.82 Å |
| 7EGJ SCP promoter-bound TFIID-TFIIA in post TBP-loading state Deposited 2021-03-24 | Assembly 1 Protein–DNA Heteromer；Protein × 22 PDB declaration: 24-meric（24） Consistent with all polymers |
Chain Q
1–376（376 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.64 Å |
| 7LBM Structure of the human Mediator-bound transcription pre-initiation complex Deposited 2021-01-08 | Assembly 1 Protein–DNA Heteromer；Protein × 54 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain M
1–376（376 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 16 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å |
| 7NVR Human Mediator with RNA Polymerase II Pre-initiation complex Deposited 2021-03-15 | Assembly 1 Protein–DNA Heteromer；Protein × 56 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain U
1–376（376 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 18 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 7NVS RNA polymerase II core pre-initiation complex with closed promoter DNA in proximal position Deposited 2021-03-16 | Assembly 1 Protein–DNA Heteromer；Protein × 20 PDB declaration: 22-meric（22） Consistent with all polymers |
Chain U
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 10 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 7NVT RNA polymerase II core pre-initiation complex with closed promoter DNA in distal position Deposited 2021-03-16 | Assembly 1 Protein–DNA Heteromer；Protein × 20 PDB declaration: 22-meric（22） Consistent with all polymers |
Chain U
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 10 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7NVU RNA polymerase II core pre-initiation complex with open promoter DNA Deposited 2021-03-16 | Assembly 1 Protein–DNA Heteromer；Protein × 20 PDB declaration: 22-meric（22） Consistent with all polymers |
Chain U
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 10 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 7NVY RNA polymerase II pre-initiation complex with closed promoter DNA in proximal position Deposited 2021-03-16 | Assembly 1 Protein–DNA Heteromer；Protein × 30 PDB declaration: 32-meric（32） Consistent with all polymers |
Chain U
1–376（376 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 17 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.30 Å |
| 7NVZ RNA polymerase II pre-initiation complex with closed promoter DNA in distal position Deposited 2021-03-16 | Assembly 1 Protein–DNA Heteromer；Protein × 30 PDB declaration: 32-meric（32） Consistent with all polymers |
Chain U
1–376（376 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 17 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.20 Å |
| 7NW0 RNA polymerase II pre-initiation complex with open promoter DNA Deposited 2021-03-16 | Assembly 1 Protein–DNA Heteromer；Protein × 30 PDB declaration: 32-meric（32） Consistent with all polymers |
Chain U
1–376（376 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 15 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 2 BEF BERYLLIUM TRIFLUORIDE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å |
| 7ZWC Structure of SNAPc:TBP-TFIIA-TFIIB sub-complex bound to U5 snRNA promoter Deposited 2022-05-19 | Assembly 1 Protein–DNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain U
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 7ZWD Structure of SNAPc containing Pol II pre-initiation complex bound to U5 snRNA promoter (CC) Deposited 2022-05-19 | Assembly 1 Protein–DNA Heteromer；Protein × 22 PDB declaration: 24-meric（24） Consistent with all polymers |
Chain U
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 11 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 7ZX7 Structure of SNAPc containing Pol II pre-initiation complex bound to U1 snRNA promoter (CC) Deposited 2022-05-20 | Assembly 1 Protein–DNA Heteromer；Protein × 22 PDB declaration: 24-meric（24） Consistent with all polymers |
Chain U
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 11 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 7ZX8 Structure of SNAPc containing Pol II pre-initiation complex bound to U1 snRNA promoter (OC) Deposited 2022-05-20 | Assembly 1 Protein–DNA Heteromer；Protein × 22 PDB declaration: 24-meric（24） Consistent with all polymers |
Chain U
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 11 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 7ZXE Structure of SNAPc containing Pol II pre-initiation complex bound to U1 snRNA promoter (OC) Deposited 2022-05-20 | Assembly 1 Protein–DNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain U
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8BVW RNA polymerase II pre-initiation complex with the distal +1 nucleosome (PIC-Nuc18W) Deposited 2022-12-20 | Assembly 1 Protein–DNA Heteromer；Protein × 40 PDB declaration: 42-meric（42） Consistent with all polymers |
Chain U
1–376（376 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 17 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 8BYQ RNA polymerase II pre-initiation complex with the proximal +1 nucleosome (PIC-Nuc10W) Deposited 2022-12-14 | Assembly 1 Protein–DNA Heteromer；Protein × 38 PDB declaration: 40-meric（40） Consistent with all polymers |
Chain U
1–376（376 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 16 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 8BZ1 RNA polymerase II core pre-initiation complex with the proximal +1 nucleosome (cPIC-Nuc10W) Deposited 2022-12-14 | Assembly 1 Protein–DNA Heteromer；Protein × 26 PDB declaration: 28-meric（28） Consistent with all polymers |
Chain U
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8GXQ PIC-Mediator in complex with +1 nucleosome (T40N) in MH-binding state Deposited 2022-09-21 | Assembly 1 Protein–DNA Heteromer；Protein × 83 PDB declaration: 87-meric（87） Consistent with all polymers |
Chain DQ
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 19 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.04 Å |
| 8GXS PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state Deposited 2022-09-21 | Assembly 1 Protein–DNA Heteromer；Protein × 83 PDB declaration: 87-meric（87） Consistent with all polymers |
Chain DQ
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 19 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.16 Å |
| 8S51 RNA polymerase II core initially transcribing complex with an ordered RNA of 8 nt Deposited 2024-02-22 | Assembly 1 Other combination Heteromer；Protein × 20 PDB declaration: 23-meric（23） Consistent with all polymers |
Chain U
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 10 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8S52 RNA polymerase II core initially transcribing complex with an ordered RNA of 10 nt Deposited 2024-02-22 | Assembly 1 Other combination Heteromer；Protein × 20 PDB declaration: 23-meric（23） Consistent with all polymers |
Chain U
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 10 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8S5N RNA polymerase II core initially transcribing complex with an ordered RNA of 12 nt Deposited 2024-02-24 | Assembly 1 Other combination Heteromer；Protein × 20 PDB declaration: 23-meric（23） Consistent with all polymers |
Chain U
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 10 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8WAK Structure of transcribing complex 2 (TC2), the initially transcribing complex with Pol II positioned 2nt downstream of TSS. Deposited 2023-09-07 | Assembly 1 Other combination Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain Q
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.47 Å |
| 8WAL Structure of transcribing complex 3 (TC3), the initially transcribing complex with Pol II positioned 3nt downstream of TSS. Deposited 2023-09-07 | Assembly 1 Other combination Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain Q
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.52 Å |
| 8WAN Structure of transcribing complex 4 (TC4), the initially transcribing complex with Pol II positioned 4nt downstream of TSS. Deposited 2023-09-07 | Assembly 1 Other combination Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain Q
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.07 Å |
| 8WAO Structure of transcribing complex 5 (TC5), the initially transcribing complex with Pol II positioned 5nt downstream of TSS. Deposited 2023-09-07 | Assembly 1 Other combination Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain Q
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.40 Å |
| 8WAP Structure of transcribing complex 6 (TC6), the initially transcribing complex with Pol II positioned 6nt downstream of TSS. Deposited 2023-09-08 | Assembly 1 Other combination Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain Q
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.85 Å |
| 8WAQ Structure of transcribing complex 7 (TC7), the initially transcribing complex with Pol II positioned 7nt downstream of TSS. Deposited 2023-09-08 | Assembly 1 Other combination Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain Q
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.29 Å |
| 8WAR Structure of transcribing complex 8 (TC8), the initially transcribing complex with Pol II positioned 8nt downstream of TSS. Deposited 2023-09-08 | Assembly 1 Other combination Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain Q
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.20 Å |
| 8WAS Structure of transcribing complex 9 (TC9), the initially transcribing complex with Pol II positioned 9nt downstream of TSS. Deposited 2023-09-08 | Assembly 1 Other combination Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain Q
1–376（376 aa）
|
Not recorded | ZN ZINC ION × 17 SF4 IRON/SULFUR CLUSTER × 1 MG MAGNESIUM ION × 1 W0F [[(2~{S},3~{R},4~{S},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3-methoxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.13 Å |