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Protein Structures and Oligomeric States

Enter a protein name, UniProt accession or PDB ID. Name searches ask you to confirm the protein identity before related structures are queried.

Current Protein Identity：P54999 New Search
36PDB entries
39Biological assembly
101chain/assembly records
Main Difference Dimensions in This Set
Different assembly state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
1N9R Crystal structure of a heptameric ring complex of yeast SmF in spacegroup P4122 Deposited 2002-11-26 Assembly 1 Protein homooligomer Homooligomer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count
Chain A 1–86（86 aa）
Chain B 1–86（86 aa）
Chain C 1–86（86 aa）
Chain D 1–86（86 aa）
Chain E 1–86（86 aa）
Chain F 1–86（86 aa）
Chain G 1–86（86 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；289 K；PEG 3350, sodium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 289K
Resolution 2.80 Å R-free 0.268
1N9R Crystal structure of a heptameric ring complex of yeast SmF in spacegroup P4122 Deposited 2002-11-26 Assembly 2 Protein homooligomer Homooligomer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count
Chain A 1–86（86 aa）
Chain B 1–86（86 aa）
Chain C 1–86（86 aa）
Chain D 1–86（86 aa）
Chain E 1–86（86 aa）
Chain F 1–86（86 aa）
Chain G 1–86（86 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；289 K；PEG 3350, sodium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 289K
Resolution 2.80 Å R-free 0.268
1N9S Crystal structure of yeast SmF in spacegroup P43212 Deposited 2002-11-26 Assembly 1 Protein homooligomer Homooligomer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count
Chain A 1–86（86 aa）
Chain B 1–86（86 aa）
Chain C 1–86（86 aa）
Chain D 1–86（86 aa）
Chain E 1–86（86 aa）
Chain F 1–86（86 aa）
Chain G 1–86（86 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；289 K；Tris, PEG 3350, sodium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 289K
Resolution 3.50 Å R-free 0.297
1N9S Crystal structure of yeast SmF in spacegroup P43212 Deposited 2002-11-26 Assembly 2 Protein homooligomer Homooligomer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count
Chain H 1–86（86 aa）
Chain I 1–86（86 aa）
Chain J 1–86（86 aa）
Chain K 1–86（86 aa）
Chain L 1–86（86 aa）
Chain M 1–86（86 aa）
Chain N 1–86（86 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；289 K；Tris, PEG 3350, sodium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 289K
Resolution 3.50 Å R-free 0.297
1N9S Crystal structure of yeast SmF in spacegroup P43212 Deposited 2002-11-26 Assembly 3 Protein homooligomer Homooligomer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count
Chain A 1–86（86 aa）
Chain B 1–86（86 aa）
Chain C 1–86（86 aa）
Chain D 1–86（86 aa）
Chain E 1–86（86 aa）
Chain F 1–86（86 aa）
Chain G 1–86（86 aa）
Chain H 1–86（86 aa）
Chain I 1–86（86 aa）
Chain J 1–86（86 aa）
Chain K 1–86（86 aa）
Chain L 1–86（86 aa）
Chain M 1–86（86 aa）
Chain N 1–86（86 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；289 K；Tris, PEG 3350, sodium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 289K
Resolution 3.50 Å R-free 0.297
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric（34） Consistent with all polymers
Chain W 1–86（86 aa）
Chain Z 1–86（86 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
5GAM Foot region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: Dodecameric（12） Consistent with all polymers
Chain f 1–86（86 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric（35） Consistent with all polymers
Chain f 1–86（86 aa）
Chain q 1–86（86 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAO Head region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 11-meric（11） Consistent with all polymers
Chain q 1–86（86 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 4.20 Å
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric（46） Consistent with all polymers
Chain h 1–86（86 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5GMK Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.4 angstrom resolution Deposited 2016-07-14 Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 45-meric（45） Consistent with all polymers
Chain h 1–86（86 aa）
Chain w 1–86（86 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；The CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5LJ3 Structure of the core of the yeast spliceosome immediately after branching Deposited 2016-07-17 Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 38-meric（38） Consistent with all polymers
Chain f 1–86（86 aa）
Chain q 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 3.80 Å
5LJ5 Overall structure of the yeast spliceosome immediately after branching. Deposited 2016-07-17 Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 45-meric（45） Consistent with all polymers
Chain f 1–86（86 aa）
Chain q 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 10.00 Å
5LQW yeast activated spliceosome Deposited 2016-08-17 Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 31-meric（31） Consistent with all polymers
Chain f 1–86（86 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5MPS Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-18 Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 30-meric（30） Consistent with all polymers
Chain f 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 3.85 Å
5MQ0 Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-19 Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 46-meric（46） Consistent with all polymers
Chain f 1–86（86 aa）
Chain q 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 4.17 Å
5NRL Structure of a pre-catalytic spliceosome Deposited 2017-04-24 Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric（58） Consistent with all polymers
Chain f 1–86（86 aa）
Chain q 1–86（86 aa）
Chain x 1–86（86 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0
cryo-EM vitrification conditions Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity.
Resolution 7.20 Å
5WSG Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution Deposited 2016-12-07 Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 45-meric（45） Consistent with all polymers
Chain H 1–86（86 aa）
Chain h 1–86（86 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；10mM Tris-HCl, pH 8.0, 75mM NaCl, 1mM Mg(OAc)2, 1mM imidazole, 0.01% NP40, 1mM TCEP, 0.5mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
5Y88 Cryo-EM structure of the intron-lariat spliceosome ready for disassembly from S.cerevisiae at 3.5 angstrom Deposited 2017-08-20 Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 44-meric（44） Consistent with all polymers
Chain c 1–86（86 aa）
Chain j 1–86（86 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.46 Å
5YLZ Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom Deposited 2017-10-20 Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 43-meric（43） Consistent with all polymers
Chain c 1–86（86 aa）
Chain j 1–86（86 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric（57） Consistent with all polymers
Chain U 1–86（86 aa）
Chain f 1–86（86 aa）
Chain j 1–86（86 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWN Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.3 angstrom (Part II: U1 snRNP region) Deposited 2018-05-16 Assembly 1 Protein–RNA Heteromer；Protein × 18 PDB declaration: eicosameric（20） Consistent with all polymers
Chain f 1–86（86 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric（60） Consistent with all polymers
Chain U 1–86（86 aa）
Chain f 1–86（86 aa）
Chain j 1–86（86 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6BK8 S. cerevisiae spliceosomal post-catalytic P complex Deposited 2017-11-07 Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric（46） Consistent with all polymers
Chain b 1–86（86 aa）
Chain m 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 5 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6EXN Post-catalytic P complex spliceosome with 3' splice site docked Deposited 2017-11-08 Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 40-meric（40） Review required
Chain f 1–86（86 aa）
Chain q 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；3 uL sample was applied to the grid, left for 30s, then blotted for 3s and immediately plunged into liquid ethane.
Resolution 3.70 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric（38） Consistent with all polymers
Chain f 1–86（86 aa）
Chain x 1–86（86 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
6J6G Cryo-EM structure of the yeast B*-a2 complex at an average resolution of 3.2 angstrom Deposited 2019-01-15 Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric（41） Consistent with all polymers
Chain h 1–86（86 aa）
Chain w 1–86（86 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6J6H Cryo-EM structure of the yeast B*-a1 complex at an average resolution of 3.6 angstrom Deposited 2019-01-15 Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric（41） Consistent with all polymers
Chain h 1–86（86 aa）
Chain w 1–86（86 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6J6N Cryo-EM structure of the yeast B*-b1 complex at an average resolution of 3.86 angstrom Deposited 2019-01-15 Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric（41） Consistent with all polymers
Chain h 1–86（86 aa）
Chain w 1–86（86 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.86 Å
6J6Q Cryo-EM structure of the yeast B*-b2 complex at an average resolution of 3.7 angstrom Deposited 2019-01-15 Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 42-meric（42） Consistent with all polymers
Chain h 1–86（86 aa）
Chain w 1–86（86 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6N7P S. cerevisiae spliceosomal E complex (UBC4) Deposited 2018-11-27 Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric（21） Consistent with all polymers
Chain P 1–86（86 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6N7R Saccharomyces cerevisiae spliceosomal E complex (ACT1) Deposited 2018-11-28 Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: octadecameric（18） Consistent with all polymers
Chain P 1–86（86 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6N7X S. cerevisiae U1 snRNP Deposited 2018-11-28 Assembly 1 Protein–RNA Heteromer；Protein × 15 PDB declaration: hexadecameric（16） Consistent with all polymers
Chain P 1–86（86 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7B9V Yeast C complex spliceosome at 2.8 Angstrom resolution with Prp18/Slu7 bound Deposited 2020-12-14 Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 50-meric（50） Consistent with all polymers
Chain f 1–86（86 aa）
Chain q 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 6 K POTASSIUM ION × 1 KGN D-chiro inositol hexakisphosphate × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric（21） Consistent with all polymers
Chain x 1–86（86 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQC The U1 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: octadecameric（18） Consistent with all polymers
Chain f 1–86（86 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric（39） Consistent with all polymers
Chain f 1–86（86 aa）
Chain x 1–86（86 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å
8W2O Yeast U1 snRNP with humanized U1C Zinc-Finger domain Deposited 2024-02-20 Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: 18-meric（18） Consistent with all polymers
Chain P 1–86（86 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；20 mM Hepes, pH7.9, 120 mM KCl, 2 mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE；The grids were obtained with the chamber at 100% humidity, 2.5 s blotting time, -6 blotting force and 15 s wait time and flash-frozen into liquid ethane with a Vitrobot Mark IV (Thermo Fisher Scientific)
Resolution 3.49 Å
9DTR Structure of the yeast post-catalytic P complex spliceosome at 2.3 Angstrom resolution Deposited 2024-10-01 Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 47-meric（47） Consistent with all polymers
Chain f 1–86（86 aa）
Chain q 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 4 IHP INOSITOL HEXAKISPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.31 Å

This page presents structural evidence recorded in PDB/mmCIF.“No recorded non-water small molecule” does not prove that the experimental sample was ligand-free; experimental conditions belong to the PDB experiment, while ligands and copy counts are shown for each biological assembly.