1rie

STRUCTURE OF A WATER SOLUBLE FRAGMENT OF THE RIESKE IRON-SULFUR PROTEIN OF THE BOVINE HEART MITOCHONDRIAL CYTOCHROME BC1-COMPLEX

Method: X-RAY DIFFRACTION D_max: 53.0 Å Quality: GOOD

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

RIESKE IRON-SULFUR PROTEIN

物种未注明

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白单体单体蛋白 × 1 PDB 声明：monomeric（1）与蛋白拷贝数一致	链 A； UniProt 146–274	片段：SOLUBLE FRAGMENT	FES FE2/S2 (INORGANIC) CLUSTER × 1	X-RAY DIFFRACTION X-ray结晶条件：pH 6；pH 6.0	分辨率 1.50 Å R-free 0.211

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly， “相对当前条目”直接指出证据层面的不同；没有差异标签表示当前已读取字段一致。

其他 PDB	相对当前条目 1RIE	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
1BCC CYTOCHROME BC1 COMPLEX FROM CHICKEN 提交 1998-03-23	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 20 PDB 声明：20-MERIC	链 A 1–4（4 aa）链 E 79–274（196 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 6 U10 UBIQUINONE-10 × 2 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 BOG octyl beta-D-glucopyranoside × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2	X-RAY DIFFRACTION X-ray结晶条件 pH 6.7；20MM KMES PH6.7, 75MM NACL, 10% GLYCEROL, AND 6% PEG4000	分辨率 3.16 Å R-free 0.310
1BE3 CYTOCHROME BC1 COMPLEX FROM BOVINE 提交 1998-05-19	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 11 PDB 声明：undecameric	链 E 79–274（196 aa）链 I 1–78（78 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6.6；pH 6.6	分辨率 3.00 Å R-free 0.320
1BE3 CYTOCHROME BC1 COMPLEX FROM BOVINE 提交 1998-05-19	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）链 I 1–78（78 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2	X-RAY DIFFRACTION X-ray结晶条件 pH 6.6；pH 6.6	分辨率 3.00 Å R-free 0.320
1BGY CYTOCHROME BC1 COMPLEX FROM BOVINE 提交 1998-06-02	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）链 I 1–78（78 aa）链 Q 79–274（196 aa）链 U 1–78（78 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6.8；pH 6.8	分辨率 3.00 Å R-free 0.360
1L0L structure of bovine mitochondrial cytochrome bc1 complex with a bound fungicide famoxadone 提交 2002-02-11	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 11 PDB 声明：undecameric	链 E 79–274（196 aa）链 I 1–78（78 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 3 FMX FAMOXADONE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.35 Å R-free 0.306
1L0L structure of bovine mitochondrial cytochrome bc1 complex with a bound fungicide famoxadone 提交 2002-02-11	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）链 I 1–78（78 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 6 FMX FAMOXADONE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.35 Å R-free 0.306
1L0N native structure of bovine mitochondrial cytochrome bc1 complex 提交 2002-02-11	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 11 PDB 声明：undecameric	链 E 79–274（196 aa）链 I 1–78（78 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 1	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.60 Å R-free 0.297
1L0N native structure of bovine mitochondrial cytochrome bc1 complex 提交 2002-02-11	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）链 I 1–78（78 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.60 Å R-free 0.297
1NTK Crystal Structure of Mitochondrial Cytochrome bc1 in Complex with Antimycin A1 提交 2003-01-30	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）链 I 1–57（57 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 6 AY1 [(2R,3S,6S,7R,8R)-3-[(3-formamido-2-oxidanyl-phenyl)carbonylamino]-8-hexyl-2,6-dimethyl-4,9-bis(oxidanylidene)-1,5-dioxonan-7-yl] 2-methylpropanoate × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 7.2；277 K；PEG 4000, ammonium acetate, potassium chloride, glycerol, DMG/SPC, MOPS, pH 7.2, VAPOR DIFFUSION, temperature 277K	分辨率 2.60 Å R-free 0.270
1NTM Crystal Structure of Mitochondrial Cytochrome bc1 Complex at 2.4 Angstrom 提交 2003-01-30	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）链 I 1–57（57 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 7.2；277 K；PEG 4000, ammonium acetate, potassium chloride, glyerol, DMG/SPC, MOPS, pH 7.2, VAPOR DIFFUSION, temperature 277K	分辨率 2.40 Å R-free 0.285
1NTZ Crystal Structure of Mitochondrial Cytochrome bc1 Complex Bound with Ubiquinone 提交 2003-01-30	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）链 I 1–57（57 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 6 UQ2 UBIQUINONE-2 × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 7.2；277 K；PEG 4000, ammonium acetate, potassium chloride, glycerol, DMG/SPC, MOPS, pH 7.2, VAPOR DIFFUSION, temperature 277K	分辨率 2.60 Å R-free 0.283
1NU1 Crystal Structure of Mitochondrial Cytochrome bc1 Complexed with 2-nonyl-4-hydroxyquinoline N-oxide (NQNO) 提交 2003-01-30	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）链 I 1–57（57 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 6 QNO 2-NONYL-4-HYDROXYQUINOLINE N-OXIDE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 7.2；277 K；PEG 4000, ammonium acetate, potassium chloride, glycerol, DMG/SPC, MOPS, pH 7.2, VAPOR DIFFUSION, temperature 277K	分辨率 3.20 Å R-free 0.296
1PP9 Bovine cytochrome bc1 complex with stigmatellin bound 提交 2003-06-16	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 20 PDB 声明：eicosameric	链 E 79–274（196 aa）链 I 1–78（78 aa）链 R 79–274（196 aa）链 V 1–78（78 aa）	未记录	JZR hexyl beta-D-glucopyranoside × 6 AZI AZIDE ION × 4 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 9 PO4 PHOSPHATE ION × 2 GOL GLYCEROL × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 SMA STIGMATELLIN A × 2 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 2 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 4	X-RAY DIFFRACTION X-ray结晶条件 pH 6.65；277 K；PEG-3350, JEFFAMINE, GLYCEROL, CACODYLATE, HEXYLGLUCOSIDE, pH 6.65, VAPOR DIFFUSION, SITTING DROP, temperature 277K	分辨率 2.10 Å R-free 0.287
1PPJ Bovine cytochrome bc1 complex with stigmatellin and antimycin 提交 2003-06-16	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 20 PDB 声明：eicosameric	链 E 79–274（196 aa）链 I 1–78（78 aa）链 R 79–274（196 aa）链 V 1–78（78 aa）	未记录	JZR hexyl beta-D-glucopyranoside × 9 PO4 PHOSPHATE ION × 5 AZI AZIDE ION × 5 GOL GLYCEROL × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 SMA STIGMATELLIN A × 2 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ANY 2-METHYL-BUTYRIC ACID 3-(3-FORMYLAMINO-2-HYDROXY-BENZOYLAMINO)-8-HEPTYL-2,6-DIMETHYL-4,9-DIOXO-[1,5]DIOXONAN-7-YL ESTER × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2	X-RAY DIFFRACTION X-ray结晶条件 pH 6.7；277 K；PEG-3350, JEFFAMINE, GLYCEROL, CACODYLATE, HEXYLGLUCOSIDE , pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 6.70	分辨率 2.10 Å R-free 0.260
1QCR CRYSTAL STRUCTURE OF BOVINE MITOCHONDRIAL CYTOCHROME BC1 COMPLEX, ALPHA CARBON ATOMS ONLY 提交 1997-05-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 11 PDB 声明：undecameric	链 E 79–274（196 aa）链 I 21–48（28 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 7.2；pH 7.2	分辨率 2.70 Å R-free 0.375
1SQB Crystal Structure Analysis of Bovine Bc1 with Azoxystrobin 提交 2004-03-18	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）链 I 1–78（78 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 6 AZO METHYL (2Z)-2-(2-{[6-(2-CYANOPHENOXY)PYRIMIDIN-4-YL]OXY}PHENYL)-3-METHOXYACRYLATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2	X-RAY DIFFRACTION X-ray结晶条件 pH 7.2；277 K；20mM ammonium acetate, 20% glycerol, 12% PEG4000, 0.5M KCl, 0.1% diheptanoyl-phosphatidylcholine, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 7.20	分辨率 2.69 Å R-free 0.288
1SQP Crystal Structure Analysis of Bovine Bc1 with Myxothiazol 提交 2004-03-19	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）片段：iron sulfur protein 链 I 1–78（78 aa）片段：subunit 9	未记录	CDL CARDIOLIPIN × 6 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 6 HEC HEME C × 6 MYX (2Z,6E)-7-{2'-[(2E,4E)-1,6-DIMETHYLHEPTA-2,4-DIENYL]-2,4'-BI-1,3-THIAZOL-4-YL}-3,5-DIMETHOXY-4-METHYLHEPTA-2,6-DIENAMID E × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 7.2；277 K；20mM ammonium acetate, 20% glycerol, 12% PEG4000, 0.5M KCl, 0.1% diheptanoyl-phosphatidylcholine, pH 7.2, VAPOR DIFFUSION, temperature 277K	分辨率 2.70 Å R-free 0.314
1SQQ Crystal Structure Analysis of Bovine Bc1 with Methoxy Acrylate Stilbene (MOAS) 提交 2004-03-19	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）片段：iron sulfur protein 链 I 1–78（78 aa）片段：subunit 9	未记录	HEC HEME C × 6 UQ2 UBIQUINONE-2 × 2 OST METHYL (2Z)-3-METHOXY-2-{2-[(E)-2-PHENYLVINYL]PHENYL}ACRYLATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 7.2；277 K；20mM ammonium acetate, 20% glycerol, 12% PEG4000, 0.5M KCl, 0.1% diheptanoyl-phosphatidylcholine , pH 7.2, VAPOR DIFFUSION, temperature 277K	分辨率 3.00 Å R-free 0.295
1SQV Crystal Structure Analysis of Bovine Bc1 with UHDBT 提交 2004-03-19	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）链 I 1–78（78 aa）	未记录	HEC HEME C × 6 UHD 6-HYDROXY-5-UNDECYL-1,3-BENZOTHIAZOLE-4,7-DIONE × 2 UQ2 UBIQUINONE-2 × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 7.2；277 K；20mM ammonium acetate, 20% glycerol, 12% PEG4000, 0.5M KCl, 0.1% diheptanoyl-phosphatidylcholine, pH 7.2, VAPOR DIFFUSION, temperature 277K	分辨率 2.85 Å R-free 0.285
1SQX Crystal Structure Analysis of Bovine Bc1 with Stigmatellin A 提交 2004-03-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）片段：cytochrome c1 链 I 1–78（78 aa）片段：subunit 7	未记录	UQ2 UBIQUINONE-2 × 2 HEC HEME C × 6 SMA STIGMATELLIN A × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 7.2；277 K；20mM ammonium acetate, 20% glycerol, 12% PEG4000, 0.5M KCl, 0.1% diheptanoyl-phosphatidylcholine , pH 7.2, VAPOR DIFFUSION, temperature 277K	分辨率 2.60 Å R-free 0.281
2A06 Bovine cytochrome bc1 complex with stigmatellin bound 提交 2005-06-16	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 20 PDB 声明：eicosameric	链 E 79–274（196 aa）链 I 1–78（78 aa）链 R 79–274（196 aa）链 V 1–78（78 aa）	未记录	JZR hexyl beta-D-glucopyranoside × 6 PO4 PHOSPHATE ION × 11 AZI AZIDE ION × 4 UNL UNKNOWN LIGAND × 74 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 SMA STIGMATELLIN A × 2 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 2 CDL CARDIOLIPIN × 4 GOL GLYCEROL × 7 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 6.65；277 K；PEG-3350, JEFFAMINE, GLYCEROL, CACODYLATE, HEXYLGLUCOSIDE , pH 6.65, VAPOR DIFFUSION, SITTING DROP, temperature 277K	分辨率 2.10 Å R-free 0.258
2FYU Crystal structure of bovine heart mitochondrial bc1 with jg144 inhibitor 提交 2006-02-08	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）片段：Iron-sulfur protein 链 I 1–78（78 aa）片段：Iron-sulfur protein signal sequence	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 6 FDN (5S)-3-ANILINO-5-(2,4-DIFLUOROPHENYL)-5-METHYL-1,3-OXAZOLIDINE-2,4-DIONE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7.2；277.2 K；50 mM MOPS 7.2pH, 20 mM Ammonium acetate, 20% glycerol.Incubation with 2-5 mol excess of JG144. Precipitant 12% PEG4000, 0.5 M KCl, 0.1% DHPC; Protein:PPT ratio 1:0.57, VAPOR DIFFUSION, SITTING DROP, temperature 277.2K	分辨率 2.26 Å R-free 0.283
2YBB Fitted model for bovine mitochondrial supercomplex I1III2IV1 by single particle cryo-EM (EMD-1876) 提交 2011-03-02	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 48 PDB 声明：48-meric	链 E 79–274（196 aa）链 I 14–78（65 aa）链 e 79–274（196 aa）链 i 14–78（65 aa）	未记录	SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 7 FES FE2/S2 (INORGANIC) CLUSTER × 4 CA CALCIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 5 SMA STIGMATELLIN A × 2 UQ1 UBIQUINONE-1 × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 4 HEA HEME-A × 2 CU COPPER (II) ION × 3 ZN ZINC ION × 1	ELECTRON MICROSCOPY cryo-EM缓冲液 10 MM KCL, 15 MM HEPES, RESIDUAL TREHALOSE；pH 7.7；10 MM KCL, 15 MM HEPES, RESIDUAL TREHALOSE cryo-EM玻璃化条件冷冻剂 ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 70, TEMPERATURE- 95, INSTRUMENT- CUSTOM-MADE HAND PLUNGER, METHOD- ONE- SIDED BLOTTING FOR 26 - 30 SECONDS BEFORE PLUNGING, DETAILS- SAMPLE WAS ADSORBED TO CARBON FILM FOR 30 SECONDS BEFORE BLOTTING	分辨率 19.00 Å
3BCC STIGMATELLIN AND ANTIMYCIN BOUND CYTOCHROME BC1 COMPLEX FROM CHICKEN 提交 1998-03-23	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 20 PDB 声明：20-MERIC	链 E 79–274（196 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 6 SIG STIGMATELLIN × 2 AMY ANTIMYCIN × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2	X-RAY DIFFRACTION X-ray结晶条件 pH 6.7；20MM KMES PH6.7, 75MM NACL, 10% GLYCEROL, AND 6% PEG4000, INHIBITOR WAS ADDED FROM ETHANOLIC SOLUTION	分辨率 3.70 Å R-free 0.321
4D6T Cytochrome bc1 bound to the 4(1H)-pyridone GW844520 提交 2014-11-14	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 10 PDB 声明：decameric	链 E 1–274（274 aa）链 I 1–274（274 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 2 4X9 3-chloro-2,6-dimethyl-5-{4-[4-(trifluoromethoxy)phenoxy]phenyl}pyridin-4-ol × 1 PO4 PHOSPHATE ION × 6 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 HEC HEME C × 1 CDL CARDIOLIPIN × 2	X-RAY DIFFRACTION X-ray结晶条件 pH 6.8；50 MM POTASSIUM PHOSPHATE BUFFER PH 6.8, 12% P4000, 1.4% HECAMEG	分辨率 3.57 Å R-free 0.252
4D6T Cytochrome bc1 bound to the 4(1H)-pyridone GW844520 提交 2014-11-14	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 10 PDB 声明：decameric	链 R 1–274（274 aa）链 V 1–274（274 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 2 4X9 3-chloro-2,6-dimethyl-5-{4-[4-(trifluoromethoxy)phenoxy]phenyl}pyridin-4-ol × 1 PO4 PHOSPHATE ION × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 HEC HEME C × 1 CDL CARDIOLIPIN × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6.8；50 MM POTASSIUM PHOSPHATE BUFFER PH 6.8, 12% P4000, 1.4% HECAMEG	分辨率 3.57 Å R-free 0.252
4D6U Cytochrome bc1 bound to the 4(1H)-pyridone GSK932121 提交 2014-11-14	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 10 PDB 声明：decameric	链 E 1–274（274 aa）链 I 1–274（274 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 2 G8U 3-chloro-6-(hydroxymethyl)-2-methyl-5-{4-[3-(trifluoromethoxy)phenoxy]phenyl}pyridin-4-ol × 1 PO4 PHOSPHATE ION × 6 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 HEC HEME C × 1 CDL CARDIOLIPIN × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 6.8；SITTING DROP VAPOUR DIFFUSION 50 MM KPI, PH 6.8, 100 MM NACL, 3 MM NAN3, 10 - 13 % PEG4000,1.4% HECAMEG	分辨率 4.09 Å R-free 0.271
4D6U Cytochrome bc1 bound to the 4(1H)-pyridone GSK932121 提交 2014-11-14	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 10 PDB 声明：decameric	链 R 1–274（274 aa）链 V 1–274（274 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 2 G8U 3-chloro-6-(hydroxymethyl)-2-methyl-5-{4-[3-(trifluoromethoxy)phenoxy]phenyl}pyridin-4-ol × 1 PO4 PHOSPHATE ION × 3 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 HEC HEME C × 1 CDL CARDIOLIPIN × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 6.8；SITTING DROP VAPOUR DIFFUSION 50 MM KPI, PH 6.8, 100 MM NACL, 3 MM NAN3, 10 - 13 % PEG4000,1.4% HECAMEG	分辨率 4.09 Å R-free 0.271
5GPN Architecture of mammalian respirasome 提交 2016-08-03	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 84 PDB 声明：84-meric	链 I 1–78（78 aa）链 U 1–78（78 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.2 cryo-EM玻璃化条件冷冻剂 NITROGEN	分辨率 5.40 Å
5KLV Structure of bos taurus cytochrome bc1 with fenamidone inhibited 提交 2016-06-25	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）链 I 1–78（78 aa）	未记录	6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 4 CDL CARDIOLIPIN × 6 GOL GLYCEROL × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 FNM (5S)-5-methyl-2-(methylsulfanyl)-5-phenyl-3-(phenylamino)-3,5-dihydro-4H-imidazol-4-one × 2 8PE (2R)-3-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(tetradecanoyloxy)propyl octadecanoate × 2 CL CHLORIDE ION × 2 HEC HEME C × 2 PEF DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7.2；277 K；50 mM MOPS 7.2 pH, 20 mM Ammonium acetate, 20% glycerol. Incubation with 2-5 mol excess of Fenamidone. Precipitant 12% PEG4000, 0.5 M KCl, 0.1% DHPC; Protein:PPT ratio 1:0.57	分辨率 2.65 Å R-free 0.269
5LUF Cryo-EM of bovine respirasome 提交 2016-09-08	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 62 PDB 声明：62-meric	链 e 79–274（196 aa）链 i 1–78（78 aa）链 q 79–274（196 aa）链 u 1–78（78 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 SF4 IRON/SULFUR CLUSTER × 6	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.7 cryo-EM玻璃化条件冷冻剂 ETHANE；10 s blotting	分辨率 9.10 Å
5NMI Cytochrome bc1 bound to the inhibitor MJM170 提交 2017-04-05	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 1–274（274 aa）链 I 1–274（274 aa）链 R 1–274（274 aa）链 V 1–274（274 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MJM (4aS)-2-methyl-3-(4-phenoxyphenyl)-5,6,7,8-tetrahydroquinolin-4(4aH)-one × 2 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 6 CDL CARDIOLIPIN × 6 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 6.8；277 K；10 % PEG4000, HECAMEG	分辨率 3.50 Å R-free 0.268
5OKD Crystal structure of bovine Cytochrome bc1 in complex with inhibitor SCR0911. 提交 2017-07-25	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 10 PDB 声明：decameric	链 E 79–274（196 aa）链 I 32–78（47 aa）	未记录	PG4 TETRAETHYLENE GLYCOL × 3 6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 1 CDL CARDIOLIPIN × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 9XE 7-methoxy-3-methyl-2-[5-[4-(trifluoromethyloxy)phenyl]pyridin-3-yl]-1~{H}-quinolin-4-one × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 HEC HEME C × 1 PO4 PHOSPHATE ION × 6 FES FE2/S2 (INORGANIC) CLUSTER × 1 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；277 K；Protein 40mg/mL with 1.6% HECAMEG; reservoir solution 50mM KPi pH 6.8, 100mM NaCl, 3mM NaN3, 10-13% PEG4000	分辨率 3.10 Å R-free 0.240
6FO0 CryoEM structure of bovine cytochrome bc1 in complex with the anti-malarial compound GSK932121 提交 2018-02-05	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 20 PDB 声明：eicosameric	链 E 1–274（274 aa）链 R 1–274（274 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 G8U 3-chloro-6-(hydroxymethyl)-2-methyl-5-{4-[3-(trifluoromethoxy)phenoxy]phenyl}pyridin-4-ol × 2 HEC HEME C × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.5 cryo-EM玻璃化条件冷冻剂 ETHANE；blot for 6 seconds with a blot force of 6 before plunge-freezing	分辨率 4.10 Å
6FO2 CryoEM structure of bovine cytochrome bc1 with no ligand bound 提交 2018-02-05	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 20 PDB 声明：eicosameric	链 E 1–274（274 aa）链 I 62–77（16 aa）链 R 1–274（274 aa）链 V 62–77（16 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.5 cryo-EM玻璃化条件冷冻剂 ETHANE；blot for 6 seconds with a blot force of 6 before plunge-freezing	分辨率 4.40 Å
6FO6 CryoEM structure of bovine cytochrome bc1 in complex with the anti-malarial inhibitor SCR0911 提交 2018-02-06	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 18 PDB 声明：octadecameric	链 E 1–274（274 aa）链 R 1–274（274 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 DY2 7-methoxy-3-methyl-2-[5-[4-(trifluoromethyloxy)phenyl]pyridin-3-yl]quinolin-4-ol × 2 HEC HEME C × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.5 cryo-EM玻璃化条件冷冻剂 ETHANE；blot for 6 seconds with a blot force of 6 before plunge-freezing	分辨率 4.10 Å
6HAW Crystal structure of bovine cytochrome bc1 in complex with 2-pyrazolyl quinolone inhibitor WDH2G7 提交 2018-08-08	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 10 PDB 声明：decameric	链 E 79–274（196 aa）链 I 33–78（46 aa）	未记录	6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 1 PGE TRIETHYLENE GLYCOL × 4 PO4 PHOSPHATE ION × 11 CDL CARDIOLIPIN × 4 PG4 TETRAETHYLENE GLYCOL × 1 PX6 1,2-DIPALMITOYL-SN-GLYCERO-3-PHOSPHATE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 FX2 7-methoxy-3-methyl-2-[1-[[4-(trifluoromethyloxy)phenyl]methyl]pyrazol-4-yl]-3~{H}-quinolin-4-one × 1 HEC HEME C × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；Protein 40mg/mL with 1.6% HECAMEG; reservoir solution 50mM KPi pH 6.8, 100mM NaCl, 3mM NaN3, 10-12% PEG4000.	分辨率 3.45 Å R-free 0.242
6NHG Rhodobacter sphaeroides Mitochondrial respiratory chain complex 提交 2018-12-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）链 I 1–78（78 aa）	未记录	6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 4 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 AZO METHYL (2Z)-2-(2-{[6-(2-CYANOPHENOXY)PYRIMIDIN-4-YL]OXY}PHENYL)-3-METHOXYACRYLATE × 2 8PE (2R)-3-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(tetradecanoyloxy)propyl octadecanoate × 2 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 MC3 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7.2；277 K；50 mM MOPS 7.2 pH, 20 mM Ammonium acetate, 20% glycerol. Incubation with 2-5 mol excess of Azoxystrobin. Precipitant 12% PEG4000, 0.5 M KCl, 0.1% DHPC; Protein:PPT ratio 1:0.57	分辨率 2.80 Å R-free 0.289
6XVF Crystal structure of bovine cytochrome bc1 in complex with tetrahydro-quinolone inhibitor JAG021 提交 2020-01-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 20 PDB 声明：eicosameric	链 E 79–274（196 aa）链 I 33–78（46 aa）	未记录	PG4 TETRAETHYLENE GLYCOL × 6 6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 2 PO4 PHOSPHATE ION × 18 CDL CARDIOLIPIN × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 LMT DODECYL-BETA-D-MALTOSIDE × 2 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 JAG 2-methyl-3-[4-[4-(trifluoromethyloxy)phenoxy]phenyl]-5,6,7,8-tetrahydro-3~{H}-quinolin-4-one × 2 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；Protein 40mg/mL with 1.6% HECAMEG; Reservoir solution: 50mM KPi pH 6.8, 100mM NaCl, 3mM NaN3, 10-13% PEG4000	分辨率 3.50 Å R-free 0.232
6ZFS Crystal structure of bovine cytochrome bc1 in complex with quinolone inhibitor WDH-1U-4 提交 2020-06-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 10 PDB 声明：decameric	链 E 79–274（196 aa）链 I 33–78（46 aa）	未记录	6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 1 PO4 PHOSPHATE ION × 7 CDL CARDIOLIPIN × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 PG4 TETRAETHYLENE GLYCOL × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 JGW 2-methyl-3-[4-[[4-(trifluoromethyloxy)phenyl]methyl]phenyl]-3~{H}-quinolin-4-one × 1 HEC HEME C × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；Protein 40mg/mL with 1.6% HECAMEG; reservoir solution 50mM KPi pH 6.8, 100mM NaCl, 3mM NaN3, 10-13% PEG4000	分辨率 3.50 Å R-free 0.246
6ZFT Crystal structure of bovine cytochrome bc1 in complex with quinolone inhibitor CK-2-68 提交 2020-06-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 10 PDB 声明：decameric	链 E 79–274（196 aa）链 I 33–78（46 aa）	未记录	PG4 TETRAETHYLENE GLYCOL × 4 6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 1 CDL CARDIOLIPIN × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 JHB 7-chloranyl-3-methyl-2-[4-[[4-(trifluoromethyloxy)phenyl]methyl]phenyl]-1~{H}-quinolin-4-one × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 PO4 PHOSPHATE ION × 6 HEC HEME C × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；Protein 40mg/mL with 1.6% HECAMEG; reservoir solution 50mM KPi pH 6.8, 100mM NaCl, 3mM NaN3, 10-13% PEG4000	分辨率 3.30 Å R-free 0.244
6ZFU Crystal structure of bovine cytochrome bc1 in complex with quinolone inhibitor RKA066 提交 2020-06-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 10 PDB 声明：decameric	链 E 79–274（196 aa）链 I 33–78（46 aa）	未记录	PG4 TETRAETHYLENE GLYCOL × 3 6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 1 CDL CARDIOLIPIN × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 JHE 3-methyl-1-oxidanyl-2-[4-[[4-(trifluoromethyloxy)phenyl]methyl]phenyl]quinolin-4-one × 1 HEC HEME C × 1 PO4 PHOSPHATE ION × 5 FES FE2/S2 (INORGANIC) CLUSTER × 1 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；Protein 40mg/mL with 1.6% HECAMEG; reservoir solution 50mM KPi pH 6.8, 100mM NaCl, 3mM NaN3, 10-13% PEG4000	分辨率 3.50 Å R-free 0.238
7DGQ Activity optimized supercomplex state1 提交 2020-11-12	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 80 PDB 声明：80-meric	链 A1 79–274（196 aa）链 B5 1–78（78 aa）链 p 79–274（196 aa）链 v 1–78（78 aa）	未记录	PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.4 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 5.00 Å
7DGR Activity optimized supercomplex state2 提交 2020-11-12	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 80 PDB 声明：80-meric	链 A0 79–274（196 aa）链 B1 1–78（78 aa）链 p 79–274（196 aa）链 v 1–78（78 aa）	未记录	FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.4 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 4.60 Å
7DGS Activity optimized supercomplex state3 提交 2020-11-12	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 80 PDB 声明：80-meric	链 A0 79–274（196 aa）链 B3 1–78（78 aa）链 p 79–274（196 aa）链 v 1–78（78 aa）	未记录	FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.4 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 7.80 Å
7DKF Activity optimized supercomplex state4 提交 2020-11-24	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 80 PDB 声明：80-meric	链 E1 79–274（196 aa）链 I1 1–78（78 aa）链 Q1 79–274（196 aa）链 U1 1–78（78 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.4 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 8.30 Å
7R3V Crystal structure of bovine Cytochrome bc1 in complex with inhibitor CK-2-67. 提交 2022-02-07	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 10 PDB 声明：decameric	链 E 79–274（196 aa）链 I 33–78（46 aa）	未记录	PG4 TETRAETHYLENE GLYCOL × 3 6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 1 CDL CARDIOLIPIN × 3 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 I2Q 3-methyl-2-[4-[[4-(trifluoromethoxy)phenyl]methyl]phenyl]-1H-quinolin-4-one × 1 DMS DIMETHYL SULFOXIDE × 1 PO4 PHOSPHATE ION × 9 HEC HEME C × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；Protein 40mg/mL with 1.6% HECAMEG; reservoir solution 50mM KPi pH 6.8, 100mM NaCl, 3mM NaN3, 9-12% PEG4000	分辨率 3.20 Å R-free 0.256
7TAY Bos Taurus Mitochondrial BC1 in complex with Pyramoxadone 提交 2021-12-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）链 I 1–78（78 aa）	未记录	6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 4 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 PQU (5S)-3-anilino-5-methyl-5-(6-phenoxypyridin-3-yl)-1,3-oxazolidine-2,4-dione × 2 8PE (2R)-3-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(tetradecanoyloxy)propyl octadecanoate × 2 PEF DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE × 2 CL CHLORIDE ION × 2 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；277 K；50 mM MOPS 7.2 pH, 20 mM Ammonium acetate, 20% glycerol. Incubation with 5 molar excess of pyramoxadone. Precipitant 12% PEG4000, 0.5 M KCL, 0.1% DHPC.	分辨率 2.95 Å R-free 0.258
7TZ6 Structure of mitochondrial bc1 in complex with ck-2-68 提交 2022-02-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）链 I 1–78（78 aa）链 R 79–274（196 aa）链 V 1–78（78 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 JHB 7-chloranyl-3-methyl-2-[4-[[4-(trifluoromethyloxy)phenyl]methyl]phenyl]-1~{H}-quinolin-4-one × 2 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.5 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.88 Å
8P65 Cytochrome bc1 complex (Bos taurus) 提交 2023-05-25	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）链 I 1–54（54 aa）链 R 79–274（196 aa）链 V 1–54（54 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.5 cryo-EM玻璃化条件冷冻剂 ETHANE；Blot time of 4s with blot force of -3	分辨率 3.00 Å
9GCX Crystal structure of bovine Cytochrome bc1 in complex with inhibitor F8 提交 2024-08-03	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 1–274（274 aa）链 I 1–274（274 aa）	未记录	LOP (1R)-2-{[(R)-(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(DODECANOYLOXY)METHYL]ETHYL (9Z)-OCTADEC-9-ENOATE × 12 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 A1IKG 2-methyl-3-(4-(4-(trifluoromethoxy)phenoxy)phenyl)-1,5,7,8-tetrahydro-4H-pyrano[4,3-b]pyridin-4-one × 2 LMT DODECYL-BETA-D-MALTOSIDE × 2 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PSC (7R,17E,20E)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSA-17,20-DIEN-1-AMINIUM 4-OXIDE × 2 SO4 SULFATE ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；50 mM KPi pH 6.8, 100 mM NaCl, 3 mM NaN3, 10-20% PEG4000	分辨率 3.52 Å R-free 0.241
9W2X Cryo-EM structure of complex III on the bovine heart submitochondrial particles, III-1 提交 2025-07-28	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）链 I 1–57（57 aa）链 R 79–274（196 aa）链 V 1–57（57 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.5 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.20 Å
9W2Y Cryo-EM structure of complex III on the bovine heart submitochondrial particles, III-2 提交 2025-07-28	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 22 PDB 声明：22-meric	链 E 79–274（196 aa）链 I 1–57（57 aa）链 P 79–274（196 aa）链 T 1–57（57 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.5 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.40 Å

共 48 个其他 PDB 条目、53 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	UCRI_BOVIN
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 1–129； UniProt 146–274

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	1rie
沉积日期 deposition_date	1996-02-23
结构标题 title	STRUCTURE OF A WATER SOLUBLE FRAGMENT OF THE RIESKE IRON-SULFUR PROTEIN OF THE BOVINE HEART MITOCHONDRIAL CYTOCHROME BC1-COMPLEX
关键词 keywords	OXIDOREDUCTASE, CYTOCHROME BC1 COMPLEX, HISTIDINE LIGANDS, RIESKE IRON-SULFUR CLUSTER, ELECTRON TRANSPORT; ELECTRON TRANSPORT
实验方法 method	X-RAY DIFFRACTION

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3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	15.20 Å
回转半径 Rg (电子) rg_electron	14.03 Å
零角强度 I(0) i0	4226580.00
分子量 molecular_weight	14382.0 kDa
排除体积 excluded_volume	17882 Å³
包络体积 envelope_volume	19636 Å³
水化壳体积 shell_volume	12009 Å³
包络直径 envelope_diameter	49.6 Å
壳层 Rg shell_rg	19.72 Å
包络 Rg envelope_rg	14.33 Å
形状 Rg shape_rg	13.94 Å
总 Rg total_rg	15.38 Å
总原子数 total_atoms	1002
残基数 n_residues	127
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	53.0 Å
Rg (实空间) rg_real	15.12 Å
Rg 误差 (实空间) rg_real_error	0.49 Å
I(0) (实空间) i0_real	4.2270e+06
I(0) 误差 (实空间) i0_real_error	5.8510e+04
Rg (倒空间) rg_reciprocal	15.13 Å
I(0) (倒空间) i0_reciprocal	4227000.0000
解质量估计 total_estimate	0.7883
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	48.9 Å
偏度 Skewness skewness	0.187
峰度 Kurtosis kurtosis	-0.398
角度范围 angular_range	— – 0.5000 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	735100.0000
实空间数据点数 n_real_points	80
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.758; Stabil: 0.998; Sysdev: 1.000; Positv: 1.000; Valcen: 0.973; Smooth: 0.000

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5. 晶体学与实验 5. Crystallography & Experiment

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6. 实体与聚合物信息 Entities & Polymers (3)

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7. 折叠分类 (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

结构域编号 domain_id	d1riea_
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.33 — ISP domain
超家族 Superfamily superfamily	b.33.1 — ISP domain
家族 Family family	b.33.1.1 — Rieske iron-sulfur protein (ISP)

CATH v4.4 (1 domains)

结构域编号 domain_id	1rieA00
类 Class class	2 — Mainly Beta
架构 Architecture architecture	102 — 3-layer Sandwich
拓扑 Topology topology	10 — Rieske Iron-sulfur Protein
同源超家族 H-superfamily homologous superfamily	10 — Rieske [2Fe-2S] iron-sulphur domain

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8. 引用文献 (5)

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晶胞边长 a length_a	32.100 Å
晶胞边长 b length_b	53.000 Å
晶胞边长 c length_c	38.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	100.30 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	2

空间群 space_group	P 1 21 1
空间群编号 int_tables_number	4
晶系 crystal_system	—

分辨率 resolution	1.500 Å
最低分辨率 resolution_low	10.000 Å
R 因子 r_factor	0.1870
R_work r_work	0.1870
R_free r_free	0.2110

描述 description	RIESKE IRON-SULFUR PROTEIN
分子量 formula_weight	14440.7 kDa
EC 编号 ec_number	1.10.2.2
来源方法 src_method	nat
拷贝数 entity_count	1

聚合物类型 type	polypeptide(L)
链编号 strand_id	A
原子数 atom_count	1169

STRUCTURE OF A WATER SOLUBLE FRAGMENT OF THE RIESKE IRON-SULFUR PROTEIN OF THE BOVINE HEART MITOCHONDRIAL CYTOCHROME BC1-COMPLEX

1. 蛋白身份与相关结构 Protein Identity & Related Structures

RIESKE IRON-SULFUR PROTEIN

当前结构中的状态

数据库中的同蛋白其他状态

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

2. 结构基本信息 2. Structure Basics

3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

5. 晶体学与实验 5. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

6. 实体与聚合物信息 Entities & Polymers (3)

实体 1 Entity 1 polymer

聚合物 Polymer

实体 2 Entity 2 non-polymer

实体 3 Entity 3 water

7. 折叠分类 (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

CATH v4.4 (1 domains)

8. 引用文献 (5)

9. 文件与曲线 (10)

Files (7)

Curves (3)

描述 description	FE2/S2 (INORGANIC) CLUSTER
分子量 formula_weight	175.8 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	167

fit	/app/data/saxs_computation/ri/1rie/1rie.fit
pddf	/app/data/pr_computation/ri/1rie/1rie.pr.out
profile	/app/data/saxs_computation/ri/1rie/1rie.dat