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2g35

NMR structure of talin-PTB in complex with PIPKI

Method: SOLUTION NMR Dmax: 52.8 Å Quality: REASONABLE
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Talin-1

Mus musculus

UniProt P26039

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 305–404 Not recorded peptide × 1 SOLUTION NMR NMR measurement conditions：pH 6.3；298 K NMR sample composition：20mM phosphate buffer, 5mM Ca2+, PH 6.3, 10% D2O, 90% H2O | 10% D2O, 90% H2O Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 2G35 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1SJ7 Crystal Structure of Talin Rod 482-655 Deposited 2004-03-03 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 482–655（174 aa） Fragment：Residues 482-655
Not recorded PT PLATINUM (II) ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；298 K；30% PEG 2000 MME, 0.1M Ammonium Acetate, 0.2M Ammonium Sulfate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.50 Å R-free 0.286
1SJ7 Crystal Structure of Talin Rod 482-655 Deposited 2004-03-03 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 482–655（174 aa） Fragment：Residues 482-655
Not recorded PT PLATINUM (II) ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；298 K；30% PEG 2000 MME, 0.1M Ammonium Acetate, 0.2M Ammonium Sulfate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.50 Å R-free 0.286
1SJ7 Crystal Structure of Talin Rod 482-655 Deposited 2004-03-03 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 482–655（174 aa） Fragment：Residues 482-655
Not recorded PT PLATINUM (II) ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；298 K；30% PEG 2000 MME, 0.1M Ammonium Acetate, 0.2M Ammonium Sulfate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.50 Å R-free 0.286
1SJ7 Crystal Structure of Talin Rod 482-655 Deposited 2004-03-03 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 482–655（174 aa） Fragment：Residues 482-655
Not recorded PT PLATINUM (II) ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；298 K；30% PEG 2000 MME, 0.1M Ammonium Acetate, 0.2M Ammonium Sulfate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.50 Å R-free 0.286
1SJ7 Crystal Structure of Talin Rod 482-655 Deposited 2004-03-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 482–655（174 aa） Fragment：Residues 482-655
Chain B 482–655（174 aa） Fragment：Residues 482-655
Not recorded PT PLATINUM (II) ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；298 K；30% PEG 2000 MME, 0.1M Ammonium Acetate, 0.2M Ammonium Sulfate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.50 Å R-free 0.286
1SJ8 Solution Structure of the R1R2 Domains of Talin Deposited 2004-03-03 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 482–789（308 aa） Fragment：Residues 482-789
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.6；295 K；0.1M Sodium Citrate, 20% PEG 6000, 0.1M MgCl2, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 2.60 Å R-free 0.297
1T01 Vinculin complexed with the VBS1 helix from talin Deposited 2004-04-07 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 605–628（24 aa） Fragment：talin (vinculin binding site 1)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；293 K；30%PEG 5000, 0.1M MES, 0.2M Ammonium Sulphate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 6.50
Resolution 2.06 Å R-free 0.303
1U89 Solution structure of VBS2 fragment of talin Deposited 2004-08-05 Different construct Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 755–889（135 aa） Fragment：vbs2 domain
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；303 K；Ionic strength (raw mmCIF value) 20mM phosphate, 50mM NaCl；Pressure ambient
NMR sample composition 1.5mM talin 755-889 U-15N, 13C; 20mM phosphate buffer, 20mM NaCl, 0.02% NaN3 | 90% H2O/10% D2O
NMR sample composition 1.5mM talin 755-889 U-15N, 13C; 20mM phosphate buffer, 20mM NaCl, 0.02% NaN3 | 100% D2O
NMR sample composition 1.5mM talin 755-889 U-15N; 20mM phosphate buffer, 20mM NaCl, 0.02% NaN3 | 90% H2O/10% D2O
NMR sample composition 1.5mM talin 755-889; 20mM phosphate buffer, 20mM NaCl, 0.02% NaN3 | 100% D2O
Resolution not provided
1Y19 Structural basis for phosphatidylinositol phosphate kinase type I-gamma binding to talin at focal adhesions Deposited 2004-11-17 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 209–410（202 aa） Fragment：F2 AND F3 SUBDOMAINS OF THE FERM DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.2；277 K；0.1 M Na/K phosphte buffer, 35% MPD, pH 6.2, VAPOR DIFFUSION, temperature 277K
Resolution 2.60 Å R-free 0.286
1Y19 Structural basis for phosphatidylinositol phosphate kinase type I-gamma binding to talin at focal adhesions Deposited 2004-11-17 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 209–410（202 aa） Fragment：F2 AND F3 SUBDOMAINS OF THE FERM DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.2；277 K；0.1 M Na/K phosphte buffer, 35% MPD, pH 6.2, VAPOR DIFFUSION, temperature 277K
Resolution 2.60 Å R-free 0.286
1Y19 Structural basis for phosphatidylinositol phosphate kinase type I-gamma binding to talin at focal adhesions Deposited 2004-11-17 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain F 209–410（202 aa） Fragment：F2 AND F3 SUBDOMAINS OF THE FERM DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.2；277 K；0.1 M Na/K phosphte buffer, 35% MPD, pH 6.2, VAPOR DIFFUSION, temperature 277K
Resolution 2.60 Å R-free 0.286
1Y19 Structural basis for phosphatidylinositol phosphate kinase type I-gamma binding to talin at focal adhesions Deposited 2004-11-17 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain H 209–410（202 aa） Fragment：F2 AND F3 SUBDOMAINS OF THE FERM DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.2；277 K；0.1 M Na/K phosphte buffer, 35% MPD, pH 6.2, VAPOR DIFFUSION, temperature 277K
Resolution 2.60 Å R-free 0.286
1Y19 Structural basis for phosphatidylinositol phosphate kinase type I-gamma binding to talin at focal adhesions Deposited 2004-11-17 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain J 209–410（202 aa） Fragment：F2 AND F3 SUBDOMAINS OF THE FERM DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.2；277 K；0.1 M Na/K phosphte buffer, 35% MPD, pH 6.2, VAPOR DIFFUSION, temperature 277K
Resolution 2.60 Å R-free 0.286
1Y19 Structural basis for phosphatidylinositol phosphate kinase type I-gamma binding to talin at focal adhesions Deposited 2004-11-17 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain L 209–410（202 aa） Fragment：F2 AND F3 SUBDOMAINS OF THE FERM DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.2；277 K；0.1 M Na/K phosphte buffer, 35% MPD, pH 6.2, VAPOR DIFFUSION, temperature 277K
Resolution 2.60 Å R-free 0.286
1ZW3 Vinculin Head (0-258) in Complex with the Talin Rod residues 1630-1652 Deposited 2005-06-03 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1628–1652（25 aa） Fragment：Residues 1630-1652
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.1；293 K；1.2M NaH2PO4/0.8M K2HPO4, 100mM CAPS, pH 10.5, pH 6.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 3.30 Å R-free 0.307
2B0H Solution structure of VBS3 fragment of talin Deposited 2005-09-14 Different construct Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1843–1973（131 aa） Fragment：residues 1843-1973
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Ionic strength (raw mmCIF value) 50 mM NaCl；Pressure ambient
NMR sample composition 1.5 mM talin 1843-1973 [15N,13C]; 20 mM phosphate, (pH 6.5), 50 mM NaCl, 2 mM DTT, 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition 1.5 mM talin 1843-1973 [15N,13C]; 20 mM phosphate, (pH 6.5), 50 mM NaCl, 2 mM DTT, 100% D2O | 100% D2O
NMR sample composition 1.0 mM talin 1843-1973 [15N]; 20 mM phosphate, (pH 6.5), 50 mM NaCl, 2 mM DTT, 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition 1.0 mM talin 1843-1973; 20 mM phosphate, (pH 6.5), 50 mM NaCl, 2 mM DTT, 90% H2O, 10% D2O | 90% H2O/10% D2O
Resolution not provided
2JSW Solution Structure of the R13 Domain of Talin Deposited 2007-07-17 Different construct Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2300–2482（183 aa） Fragment：Talin1 residues 2300-2482
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6；318 K；Ionic strength (raw mmCIF value) 50；Pressure ambient
NMR sample composition 1 mM Talin c-terminal actin binding site (ABS3), 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-15N] Talin c-terminal actin binding site (ABS3), 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-13C; U-15N] Talin c-terminal actin binding site (ABS3), 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2KBB Solution Structure of the R9 Domain of Talin Deposited 2008-11-24 Different construct Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1655–1822（168 aa） Fragment：RESIDUES 1655-1822
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；308 K；Ionic strength (raw mmCIF value) 50；Pressure AMBIENT
NMR sample composition 1 mM [U-100% 15N] TALIN-1, 10 % [U-100% 2H] D2O, 50 mM sodium chloride, 2 mM DTT, 50 mM sodium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-100% 13C; U-100% 15N] TALIN-1, 10 % [U-100% 2H] D2O, 50 mM sodium chloride, 2 mM DTT, 50 mM sodium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2KC2 NMR structure of the F1 domain (residues 86-202) of the talin Deposited 2008-12-13 Different construct Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 86–202（117 aa） Fragment：F1 domain (residues 86-202)
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Ionic strength (raw mmCIF value) 50；Pressure ambient
NMR sample composition 0.6 mM [U-100% 13C; U-100% 15N] F1-1, 10 % [U-100% 2H] D2O-2, 2 mM DTT-3, 50 mM sodium chloride-4, 20 mM sodium phosphate-5, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 0.6 mM [U-100% 15N] F1-6, 10 % [U-100% 2H] D2O-7, 2 mM DTT-8, 50 mM sodium chloride-9, 20 mM sodium phosphate-10, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2KGX HADDOCK structure of the talin F3 domain in complex with talin 1655-1822 Deposited 2009-03-23 Different construct Different mutation/modification Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1655–1822（168 aa）
Mutation：C336S No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Ionic strength (raw mmCIF value) 50；Pressure ambient
NMR sample composition 0.5 mM [U-100% 15N] 1655-1822, 2 mM F3, 50 mM sodium chloride, 20 mM sodium phosphate, 2 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 0.5 mM 1655-1822, 2 mM [U-100% 15N] F3, 50 mM sodium chloride, 20 mM sodium phosphate, 2 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2KVP Solution Structure of the R10 Domain of Talin Deposited 2010-03-24 Different construct Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1815–1973（159 aa） Fragment：VBS3 DOMAIN (UNP RESIDUES 1815-1973)
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；303 K；Ionic strength (raw mmCIF value) 50；Pressure AMBIENT
NMR sample composition 1 mM [U-100% 15N] TALIN-1, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-100% 13C; U-100% 15N] TALIN-1, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2L10 Solution Structure of the R6 Domain of Talin Deposited 2010-07-22 Different construct Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1206–1357（152 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Ionic strength (raw mmCIF value) 50；Pressure ambient
NMR sample composition 1 mM [U-100% 15N] C-1, 5 % [U-100% 2H] D2O-2, 2 mM DTT-3, 50 mM sodium chloride-4, 20 mM TRIS-5, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-100% 13C; U-100% 15N] C-6, 5 % [U-100% 2H] D2O-7, 2 mM DTT-8, 50 mM sodium chloride-9, 20 mM TRIS-10, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2L7A Solution Structure of the R3 Domain of Talin Deposited 2010-12-06 Different construct Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 787–911（125 aa） Fragment：Vbs2b domain, residues 787-911
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Ionic strength (raw mmCIF value) 50；Pressure ambient
NMR sample composition 1 mM [U-100% 15N] Vbs2b-1; 10 % [U-100% 2H] D2O-2; 50 mM sodium chloride-3; 2 mM DTT-4, 20 mM TRIS-5; 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-100% 13C; U-100% 15N] Vbs2b-6; 10 % [U-100% 2H] D2O-7; 50 mM sodium chloride-8; 2 mM DTT-9; 20 mM TRIS-10, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-100% 15N] Vbs2b-11, 100 % [U-100% 2H] D2O-12; 50 mM sodium chloride-13; 2 mM DTT-14; 20 mM TRIS-15; 100% D2O | 100% D2O
Resolution not provided
2L7N Solution Structure of the R5 Domain of Talin Deposited 2010-12-14 Different construct Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1046–1207（162 aa） Fragment：sequence database residues 1046-1207
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Ionic strength (raw mmCIF value) 50；Pressure ambient
NMR sample composition 1 mM [U-100% 15N] DomB, 10 % [U-100% 2H] D2O, 90 % H2O, 2 mM DTT, 50 mM sodium chloride, 20 mM sodium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-100% 13C; U-100% 15N] DomB, 10 % [U-100% 2H] D2O, 90 % H2O, 2 mM DTT, 50 mM sodium chloride, 20 mM sodium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2LQG Solution Structure of the R4 domain of talin Deposited 2012-03-06 Different construct Different oligomeric state Different experimental conditions Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 913–1044（132 aa） Fragment：UNP residues 913-1044
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.5；298 K；Ionic strength (raw mmCIF value) 50；Pressure ambient
NMR sample composition 0.8 mM [U-100% 15N] Domain A, 10 % [U-100% 2H] D2O, 50 mM sodium chloride, 20 mM sodium phosphate, 2 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 0.8 mM [U-100% 13C; U-100% 15N] Domain A, 10 % [U-100% 2H] D2O, 50 mM sodium chloride, 20 mM sodium phosphate, 2 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2QDQ Crystal structure of the talin dimerisation domain Deposited 2007-06-21 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2494–2541（48 aa） Fragment：Reidues 2494-2541, dimerization domain
Chain B 2494–2541（48 aa） Fragment：Reidues 2494-2541, dimerization domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.2；292 K；100 mM Citrate buffer (pH 4.2), 11% (w/v) PEG 3000, 200 mM Sodium Chloride, VAPOR DIFFUSION, SITTING DROP, temperature 292K
Resolution 2.20 Å R-free 0.315
2X0C Crystal Structure of the R7R8 domains of Talin Deposited 2009-12-08 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1359–1659（301 aa） Fragment：RESIDUES 1359-1659
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；18% PEG 8K, 100MM TRIS, 180MM SODIUM PHOSPHATE DIBASIC, PH 8.5
Resolution 2.00 Å R-free 0.264
3DYJ Crystal Structure of the R11R12 Domains of Talin Deposited 2008-07-28 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1974–2293（320 aa） Fragment：TALIN ROD (UNP RESIDUES:1974-2293)
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；10% PEG 8000, 1% PEG 3350, 100MM HEPES (PH 7.5), 10MM SODIUM THIOCYANATE, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 273K, pH 7.50, temperature 293K
Resolution 1.85 Å R-free 0.260
3DYJ Crystal Structure of the R11R12 Domains of Talin Deposited 2008-07-28 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1974–2293（320 aa） Fragment：TALIN ROD (UNP RESIDUES:1974-2293)
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；10% PEG 8000, 1% PEG 3350, 100MM HEPES (PH 7.5), 10MM SODIUM THIOCYANATE, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 273K, pH 7.50, temperature 293K
Resolution 1.85 Å R-free 0.260
3IVF Crystal structure of the talin head FERM domain Deposited 2009-09-01 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–400（400 aa） Fragment：N-terminal FERM domain, UNP residues 1-400
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.4；277 K；21% PEG4000, 0.1M Tris, 0.2M ammonium sulphate, pH7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 1.94 Å R-free 0.257
3S90 Human vinculin head domain Vh1 (residues 1-252) in complex with murine talin (VBS33; residues 1512-1546) Deposited 2011-05-31 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1512–1546（35 aa） Fragment：unp residues 1512-1546
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.3；277 K；0.1 M citrate 5.3, 15% (w/v) PEG 10K, 1.5% (v/v) dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.97 Å R-free 0.210
3S90 Human vinculin head domain Vh1 (residues 1-252) in complex with murine talin (VBS33; residues 1512-1546) Deposited 2011-05-31 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 1512–1546（35 aa） Fragment：unp residues 1512-1546
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.3；277 K；0.1 M citrate 5.3, 15% (w/v) PEG 10K, 1.5% (v/v) dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 1.97 Å R-free 0.210
4F7G Crystal structure of talin autoinhibition complex Deposited 2012-05-16 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 206–405（200 aa） Fragment：F2F3 subdomain, UNP residues 206-405
Chain B 1654–1847（194 aa） Fragment：RS subdomain, UNP residues 1654-1847
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；289 K；18.5% (w/v) PEG 1500, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；289 K；18.5% (w/v) PEG 1500, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 2.05 Å R-free 0.249
4W8P Crystal structure of RIAM TBS1 in complex with talin R7R8 domains Deposited 2014-08-25 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1357–1657（301 aa）
Not recorded EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；100 mM NaCl, 20% (w/v) polyethylene glycol 3350 and 20% (v/v) ethylene glycol
Resolution 1.50 Å R-free 0.242
4W8P Crystal structure of RIAM TBS1 in complex with talin R7R8 domains Deposited 2014-08-25 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1357–1657（301 aa）
Not recorded EDO 1,2-ETHANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；100 mM NaCl, 20% (w/v) polyethylene glycol 3350 and 20% (v/v) ethylene glycol
Resolution 1.50 Å R-free 0.242
5FZT The crystal structure of R7R8 in complex with a DLC1 fragment. Deposited 2016-03-15 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1359–1659（301 aa） Fragment：R7R8, UNP RESIDUES 1359-1659
Not recorded MLI MALONATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.4；15% ETHANOL, 0.1 M TRIS PH 7.4 AT 4OC
Resolution 2.10 Å R-free 0.231
5IC0 Structural analysis of a talin triple domain module Deposited 2016-02-22 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1357–1822（466 aa）
Not recorded EDO 1,2-ETHANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M MES pH6.5, 21% (w/v) PEG 3350, 2% (w/v) benzamidine hydrochloride
Resolution 1.97 Å R-free 0.230
5IC1 Structural analysis of a talin triple domain module, E1794Y, E1797Y, Q1801Y mutant Deposited 2016-02-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1357–1822（466 aa）
Mutation：E1794Y, E1797Y, Q1801Y EDO 1,2-ETHANEDIOL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；0.2 M potassium phosphate, 20% (w/v) PEG 3350
Resolution 2.20 Å R-free 0.249
5NL1 Shigella IpaA-VBS3/TBS in complex with the Talin VBS1 domain 488-512 Deposited 2017-04-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 480–635（156 aa） Fragment：UNP residues 480-635
Chain B 480–635（156 aa） Fragment：UNP residues 480-635
Not recorded EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；292 K；0.2 M ammonium sulfate 0.1 M sodium acetate pH 4.6 30 % PEG 4000
Resolution 2.50 Å R-free 0.228
5NL1 Shigella IpaA-VBS3/TBS in complex with the Talin VBS1 domain 488-512 Deposited 2017-04-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain C 480–635（156 aa） Fragment：UNP residues 480-635
Chain E 480–635（156 aa） Fragment：UNP residues 480-635
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；292 K；0.2 M ammonium sulfate 0.1 M sodium acetate pH 4.6 30 % PEG 4000
Resolution 2.50 Å R-free 0.228
5NL1 Shigella IpaA-VBS3/TBS in complex with the Talin VBS1 domain 488-512 Deposited 2017-04-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain D 480–635（156 aa） Fragment：UNP residues 480-635
Chain F 480–635（156 aa） Fragment：UNP residues 480-635
Not recorded CL CHLORIDE ION × 1 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；292 K；0.2 M ammonium sulfate 0.1 M sodium acetate pH 4.6 30 % PEG 4000
Resolution 2.50 Å R-free 0.228
6BA6 Solution structure of Rap1b/talin complex Deposited 2017-10-12 Different construct Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–86（86 aa） Fragment：F0 domain
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.6；301 K；Ionic strength (raw mmCIF value) 50mM NaCl, 5mM MgCl2；Pressure 101325
NMR measurement conditions pH 6.6；301 K；Ionic strength (raw mmCIF value) 50mM NaCl, 5mM MgCl2；Pressure 101325
NMR sample composition 0.6 mM [U-13C; U-15N] Rap1b, 0.9 mM talin-F0, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition 0.5 mM [U-13C; U-15N] talin-F0, 0.7 mM Rap1b, 95% H20, 5% D2O | 95% H20, 5% D2O
NMR sample composition 0.5 mM [U-15N; U-2H] talin-F0, 0.7 mM Rap1b, 95% H20, 5% D2O | 95% H20, 5% D2O
NMR sample composition 0.5 mM [U-13C; U-15N] talin-F0, 0.7 mM Rap1b, 0.2% H20, 99.8% D20 | 0.2% H20, 99.8% D20
NMR sample composition 0.6 mM [U-13C; U-15N] Rap1b, 95% H2O/5% D2O | 95% H2O/5% D2O
Resolution not provided
6MFS Mouse talin1 residues 1-138 fused to residues 169-400 in complex with phosphatidylinositol 4,5-bisphosphate (PIP2) Deposited 2018-09-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–138（138 aa） Fragment：FERM domain
Chain A 169–400（232 aa） Fragment：FERM domain
Not recorded PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 1 PO4 PHOSPHATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；18-25% PEG 3350, 200 mM Ammonium citrate pH 7.0
Resolution 2.85 Å R-free 0.290
6TWN Crystal structure of Talin1 R7R8 in complex with CDK1 (206-223) Deposited 2020-01-13 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1359–1659（301 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.6；277 K；0.1M Na citrate pH 5.6, 20% Isopropanol and 20% PEG4K
Resolution 2.28 Å R-free 0.262
6TWN Crystal structure of Talin1 R7R8 in complex with CDK1 (206-223) Deposited 2020-01-13 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1359–1659（301 aa）
Not recorded GOL GLYCEROL × 2 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.6；277 K；0.1M Na citrate pH 5.6, 20% Isopropanol and 20% PEG4K
Resolution 2.28 Å R-free 0.262
6VGU Crystal structure of FERM-folded talin head domain bound to the NPLY motif of beta3-integrin Deposited 2020-01-09 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–430（430 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.2 M sodium chloride 0.1 M MES pH6.5 10 % w/v PEG 4000
Resolution 2.78 Å R-free 0.263
7V1A Stapled TBS peptide from RIAM bound to talin R7R8 domains Deposited 2022-05-11 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1357–1657（301 aa） Fragment：R7R8 domain
Not recorded EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；293 K；100 mM NaCl, 20% (w/v) polyethylene glycol 3350 and 20% (v/v) ethylene glycol.
Resolution 1.84 Å R-free 0.254
7ZW4 Crystal structure of Talin R7R8 domains with Caskin-2 LD-peptide Deposited 2022-05-18 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1358–1659（302 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；293 K；0.1 M bis-Tris propane pH 7.0, 0.1 M NaBr and 24% PEG3350
Resolution 2.72 Å R-free 0.269
8AS9 Crystal structure of the talin-KANK1 complex Deposited 2022-08-18 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 1359–1659（301 aa）
Not recorded SO4 SULFATE ION × 12 GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；277.15 K；1M Ammonium sulfate, 0.1M CHES 9.5, 0.2M Sodium chloride, 6 % (v/v) Glycerol
Resolution 3.40 Å R-free 0.285
8FSE Crystal structure of integrin beta-2 tail bound to the FERM-folded talin head domain Deposited 2023-01-09 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–430（430 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；300 K；PEG4000 16%, NaCl 0.1M, DTT 2mM
Resolution 1.90 Å R-free 0.220
8FSE Crystal structure of integrin beta-2 tail bound to the FERM-folded talin head domain Deposited 2023-01-09 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–430（430 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；300 K；PEG4000 16%, NaCl 0.1M, DTT 2mM
Resolution 1.90 Å R-free 0.220
8FSO Crystal structure of integrin beta-6 tail bound to the FERM-folded talin head domain Deposited 2023-01-10 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–430（430 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；300 K；PEG3350 20%, NaCl 0.1M, DTT 2mM
Resolution 2.33 Å R-free 0.252
8FTB Crystal structure of integrin beta-2 tail bound to the FERM-folded talin head domain with a K306Q mutation Deposited 2023-01-11 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–430（430 aa）
Mutation：K306Q No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；300 K；PEG4000 15%, NaCl 0.1M, DTT 2mM
Resolution 1.97 Å R-free 0.211
8IVZ Crystal structure of talin R7 in complex with KANK1 KN motif Deposited 2023-03-29 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1357–1653（297 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；25% w/v pentaerythritol ethoxylate, 50 mM Magnesium chloride, 10 mM Tris pH8.5
Resolution 2.80 Å R-free 0.264
8IVZ Crystal structure of talin R7 in complex with KANK1 KN motif Deposited 2023-03-29 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1357–1653（297 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；25% w/v pentaerythritol ethoxylate, 50 mM Magnesium chloride, 10 mM Tris pH8.5
Resolution 2.80 Å R-free 0.264
8T0A Crystal structure of integrin beta-2 tail bound to the FERM-folded talin head domain with a Y270E mutation Deposited 2023-05-31 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–430（430 aa）
Mutation：Y270E No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；300 K；PEG4000 15%, NaCl 0.1M, DTT 2mM
Resolution 2.14 Å R-free 0.230
8T0D Crystal structure of integrin beta-2 tail bound to the FERM-folded talin head domain with E269A/Y270A/K306Q triple mutation Deposited 2023-05-31 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–430（430 aa）
Mutation：E269A,Y270A,K306Q No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；PEG4000 15%, NaCl 0.1M, DTT 2mM
Resolution 2.77 Å R-free 0.243
8VDO Cryogenic electron microscopy model of full-length talin lacking F2, R12 and FABD. Deposited 2023-12-16 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–2541（2541 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VDP Cryogenic electron microscopy model of full-length talin without FABD Deposited 2023-12-17 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–2541（2541 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8VDQ Cryogenic electron microscopy model of full-length talin Deposited 2023-12-17 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–2541（2541 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.50 Å
8VDR Cryogenic electron microscopy model of full-length talin without R12 and FABD Deposited 2023-12-17 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–2541（2541 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9AZ6 F-actin-Talin(R13-DD) complex Deposited 2024-03-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain F 2301–2541（241 aa） Fragment：C-terminal fragment (Domains R13-DD)
Chain G 2301–2541（241 aa） Fragment：C-terminal fragment (Domains R13-DD)
Chain H 2301–2541（241 aa） Fragment：C-terminal fragment (Domains R13-DD)
Chain I 2301–2541（241 aa） Fragment：C-terminal fragment (Domains R13-DD)
Chain J 2301–2541（241 aa） Fragment：C-terminal fragment (Domains R13-DD)
Chain K 2301–2541（241 aa） Fragment：C-terminal fragment (Domains R13-DD)
Chain L 2301–2541（241 aa） Fragment：C-terminal fragment (Domains R13-DD)
Chain M 2301–2541（241 aa） Fragment：C-terminal fragment (Domains R13-DD)
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；3.0 ul sample, blotted for 5 s from both sides with filter paper Whatman No.1
Resolution 2.98 Å
9C1T Crystal structure of integrin beta-3 tail bound to the FERM-folded talin head domain with a D397R mutation Deposited 2024-05-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–430（430 aa）
Mutation：D397R SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；0.2 M sodium chloride, 0.1 M MES pH6.5, 10 % w/v PEG 4000
Resolution 2.30 Å R-free 0.267
9DZ5 Crystal structure of integrin beta-2 tail bound to the FERM-folded talin head domain with a D397R mutation Deposited 2024-10-15 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–430（430 aa）
Mutation：D397R No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；300 K；PEG4000 16%, NaCl 0.1M, DTT 2mM
Resolution 1.74 Å R-free 0.224
9QN7 Structure of talin in complex with a peptide fragment Deposited 2025-03-24 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1974–2293（320 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.4；294.15 K；0.9M sodium potassium tartrate tetrathydrate, 20% w/v glycerol, 0.05M HEPES pH 7.4
Resolution 2.76 Å R-free 0.296
9QN7 Structure of talin in complex with a peptide fragment Deposited 2025-03-24 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1974–2293（320 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.4；294.15 K；0.9M sodium potassium tartrate tetrathydrate, 20% w/v glycerol, 0.05M HEPES pH 7.4
Resolution 2.76 Å R-free 0.296
9QT7 Crystal structure of affitin C10 fused to a coiled-coil domain in complex with a quinoline oligoamide foldamer Deposited 2025-04-08 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 2498–2529（32 aa）
Chain B 2498–2529（32 aa）
Chain C 2498–2529（32 aa）
Chain D 2498–2529（32 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；20% PEG 400 0.1M Sodium cacodylate pH 6.5
Resolution 2.80 Å R-free 0.296
9QT7 Crystal structure of affitin C10 fused to a coiled-coil domain in complex with a quinoline oligoamide foldamer Deposited 2025-04-08 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2498–2529（32 aa）
Chain D 2498–2529（32 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；20% PEG 400 0.1M Sodium cacodylate pH 6.5
Resolution 2.80 Å R-free 0.296
9QT7 Crystal structure of affitin C10 fused to a coiled-coil domain in complex with a quinoline oligoamide foldamer Deposited 2025-04-08 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 2498–2529（32 aa）
Chain C 2498–2529（32 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；20% PEG 400 0.1M Sodium cacodylate pH 6.5
Resolution 2.80 Å R-free 0.296
9SR0 Mouse vinculin head domain 1 (VD1) (residues 2-258) with A50I mutation in complex with mouse talin 1 helix 50 (H50, residues 2072-2103) Deposited 2025-09-23 Different construct Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 2072–2103（32 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；292.15 K；100 mM Bis-tris pH 6.0, 15% PEG 3350
Resolution 1.49 Å R-free 0.235

50 other PDB entries and 70 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name TLN1_MOUSE
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–100； UniProt 305–404

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 2g35

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 2g35
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2. Structure Basics 2. Structure Basics

Entry ID entry_id2g35
Deposition date deposition_date2006-02-17
Structure title titleNMR structure of talin-PTB in complex with PIPKI
Keywords keywordsTALIN, PTB DOMAIN, PIPKI, STRUCTURAL PROTEIN; STRUCTURAL PROTEIN
Experimental Method methodSOLUTION NMR
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier14.27 Å
Radius of gyration Rg (electron density) rg_electron14.32 Å
Forward intensity I(0) i0771303000.00
Molecular weight molecular_weight252270.0 kDa
Excluded volume excluded_volume322710 ų
Envelope volume envelope_volume27425 ų
Hydration-shell volume shell_volume14578 ų
Envelope diameter envelope_diameter59.9 Å
Shell Rg shell_rg21.86 Å
Envelope Rg envelope_rg16.52 Å
Shape Rg shape_rg14.24 Å
Total Rg total_rg14.76 Å
Total atoms total_atoms36100
Residues n_residues2140
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax52.8 Å
Rg (real space) rg_real14.21 Å
Rg uncertainty (real space) rg_real_error0.47 Å
I(0) (real space) i0_real7.7130e+08
I(0) uncertainty (real space) i0_real_error9.9650e+06
Rg (reciprocal space) rg_reciprocal14.22 Å
I(0) (reciprocal space) i0_reciprocal771300000.0000
Solution quality estimate total_estimate0.7344
Solution quality rating solution_quality REASONABLE a REASONABLE solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary19.4 Å
Skewness Skewness skewness0.227
Kurtosis Kurtosis kurtosis0.068
Angular range angular_range— – 0.5000 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha199500.0000
Real-space data points n_real_points80
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.526; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.966; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (2)

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7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_idd2g35a1
Class classb — All beta proteins
Fold Fold foldb.55 — PH domain-like barrel
Superfamily Superfamily superfamilyb.55.1 — PH domain-like
Family Family familyb.55.1.5 — Third domain of FERM

CATH v4.4 (1 domains)

Domain ID domain_id2g35A00
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology29 — PH-domain like
Homologous superfamily homologous superfamily30 — Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
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8. Citations (1)

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9. Files and Curves (10)