Cytochrome b-c1 complex subunit 1, mitochondrial
OrganismNot specified
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein heterocomplex Heteromer Protein × 22 PDB declaration: 22-meric（22） Consistent with protein copy count | Chain A； UniProt 36–479 Chain N； UniProt 36–479 | Not recorded | Cytochrome b-c1 complex subunit 2, mitochondrial × 2 （P23004） Cytochrome b × 2 （P00157） Cytochrome c1, heme protein, mitochondrial × 2 （P00125） Cytochrome b-c1 complex subunit Rieske, mitochondrial × 4 （P13272） Cytochrome b-c1 complex subunit 7 × 2 （P00129） Cytochrome b-c1 complex subunit 8 × 2 （P13271） Cytochrome b-c1 complex subunit 6, mitochondrial × 2 （P00126） Cytochrome b-c1 complex subunit 9 × 2 （P00130） ARG-ASN-TRP-VAL-PRO-THR-ALA-GLN-LEU-TRP-GLY-ALA-VAL-GLY-ALA-VAL-GLY-LEU-VAL-SER-ALA-THR × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MJM (4aS)-2-methyl-3-(4-phenoxyphenyl)-5,6,7,8-tetrahydroquinolin-4(4aH)-one × 2 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 6 CDL CARDIOLIPIN × 6 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 6.8；277 K；10 % PEG4000, HECAMEG | Resolution 3.50 Å R-free 0.268 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 5NMI | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1BE3 CYTOCHROME BC1 COMPLEX FROM BOVINE Deposited 1998-05-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric |
Chain A
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.6；pH 6.6
|
Resolution 3.00 Å R-free 0.320 |
| 1BE3 CYTOCHROME BC1 COMPLEX FROM BOVINE Deposited 1998-05-19 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.6；pH 6.6
|
Resolution 3.00 Å R-free 0.320 |
| 1BGY CYTOCHROME BC1 COMPLEX FROM BOVINE Deposited 1998-06-02 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
35–480（446 aa）
Chain M
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.8；pH 6.8
|
Resolution 3.00 Å R-free 0.360 |
| 1L0L structure of bovine mitochondrial cytochrome bc1 complex with a bound fungicide famoxadone Deposited 2002-02-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric |
Chain A
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 3 FMX FAMOXADONE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.35 Å R-free 0.306 |
| 1L0L structure of bovine mitochondrial cytochrome bc1 complex with a bound fungicide famoxadone Deposited 2002-02-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 6 FMX FAMOXADONE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.35 Å R-free 0.306 |
| 1L0N native structure of bovine mitochondrial cytochrome bc1 complex Deposited 2002-02-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric |
Chain A
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 3 FES FE2/S2 (INORGANIC) CLUSTER × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.60 Å R-free 0.297 |
| 1L0N native structure of bovine mitochondrial cytochrome bc1 complex Deposited 2002-02-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.60 Å R-free 0.297 |
| 1NTK Crystal Structure of Mitochondrial Cytochrome bc1 in Complex with Antimycin A1 Deposited 2003-01-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 6 AY1 [(2R,3S,6S,7R,8R)-3-[(3-formamido-2-oxidanyl-phenyl)carbonylamino]-8-hexyl-2,6-dimethyl-4,9-bis(oxidanylidene)-1,5-dioxonan-7-yl] 2-methylpropanoate × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.2；277 K；PEG 4000, ammonium acetate, potassium chloride, glycerol, DMG/SPC, MOPS, pH 7.2, VAPOR DIFFUSION, temperature 277K
|
Resolution 2.60 Å R-free 0.270 |
| 1NTM Crystal Structure of Mitochondrial Cytochrome bc1 Complex at 2.4 Angstrom Deposited 2003-01-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.2；277 K；PEG 4000, ammonium acetate, potassium chloride, glyerol, DMG/SPC, MOPS, pH 7.2, VAPOR DIFFUSION, temperature 277K
|
Resolution 2.40 Å R-free 0.285 |
| 1NTZ Crystal Structure of Mitochondrial Cytochrome bc1 Complex Bound with Ubiquinone Deposited 2003-01-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 6 UQ2 UBIQUINONE-2 × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.2；277 K；PEG 4000, ammonium acetate, potassium chloride, glycerol, DMG/SPC, MOPS, pH 7.2, VAPOR DIFFUSION, temperature 277K
|
Resolution 2.60 Å R-free 0.283 |
| 1NU1 Crystal Structure of Mitochondrial Cytochrome bc1 Complexed with 2-nonyl-4-hydroxyquinoline N-oxide (NQNO) Deposited 2003-01-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 6 QNO 2-NONYL-4-HYDROXYQUINOLINE N-OXIDE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.2；277 K；PEG 4000, ammonium acetate, potassium chloride, glycerol, DMG/SPC, MOPS, pH 7.2, VAPOR DIFFUSION, temperature 277K
|
Resolution 3.20 Å R-free 0.296 |
| 1PP9 Bovine cytochrome bc1 complex with stigmatellin bound Deposited 2003-06-16 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric |
Chain A
35–480（446 aa）
Chain N
35–480（446 aa）
|
Not recorded | JZR hexyl beta-D-glucopyranoside × 6 AZI AZIDE ION × 4 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 9 PO4 PHOSPHATE ION × 2 GOL GLYCEROL × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 SMA STIGMATELLIN A × 2 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 2 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 CDL CARDIOLIPIN × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.65；277 K；PEG-3350, JEFFAMINE, GLYCEROL, CACODYLATE, HEXYLGLUCOSIDE, pH 6.65, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.10 Å R-free 0.287 |
| 1PPJ Bovine cytochrome bc1 complex with stigmatellin and antimycin Deposited 2003-06-16 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric |
Chain A
35–480（446 aa）
Chain N
35–480（446 aa）
|
Not recorded | JZR hexyl beta-D-glucopyranoside × 9 PO4 PHOSPHATE ION × 5 AZI AZIDE ION × 5 GOL GLYCEROL × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 SMA STIGMATELLIN A × 2 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 ANY 2-METHYL-BUTYRIC ACID 3-(3-FORMYLAMINO-2-HYDROXY-BENZOYLAMINO)-8-HEPTYL-2,6-DIMETHYL-4,9-DIOXO-[1,5]DIOXONAN-7-YL ESTER × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.7；277 K；PEG-3350, JEFFAMINE, GLYCEROL, CACODYLATE, HEXYLGLUCOSIDE , pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 6.70
|
Resolution 2.10 Å R-free 0.260 |
| 1QCR CRYSTAL STRUCTURE OF BOVINE MITOCHONDRIAL CYTOCHROME BC1 COMPLEX, ALPHA CARBON ATOMS ONLY Deposited 1997-05-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric |
Chain A
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.2；pH 7.2
|
Resolution 2.70 Å R-free 0.375 |
| 1SQB Crystal Structure Analysis of Bovine Bc1 with Azoxystrobin Deposited 2004-03-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
1–480（480 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 6 AZO METHYL (2Z)-2-(2-{[6-(2-CYANOPHENOXY)PYRIMIDIN-4-YL]OXY}PHENYL)-3-METHOXYACRYLATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.2；277 K；20mM ammonium acetate, 20% glycerol, 12% PEG4000, 0.5M KCl, 0.1% diheptanoyl-phosphatidylcholine, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 7.20
|
Resolution 2.69 Å R-free 0.288 |
| 1SQP Crystal Structure Analysis of Bovine Bc1 with Myxothiazol Deposited 2004-03-19 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
1–480（480 aa）
Fragment：core protein 1
|
Not recorded | CDL CARDIOLIPIN × 6 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 6 HEC HEME C × 6 MYX (2Z,6E)-7-{2'-[(2E,4E)-1,6-DIMETHYLHEPTA-2,4-DIENYL]-2,4'-BI-1,3-THIAZOL-4-YL}-3,5-DIMETHOXY-4-METHYLHEPTA-2,6-DIENAMID E × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PLX (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.2；277 K；20mM ammonium acetate, 20% glycerol, 12% PEG4000,
0.5M KCl, 0.1% diheptanoyl-phosphatidylcholine, pH 7.2, VAPOR DIFFUSION, temperature 277K
|
Resolution 2.70 Å R-free 0.314 |
| 1SQQ Crystal Structure Analysis of Bovine Bc1 with Methoxy Acrylate Stilbene (MOAS) Deposited 2004-03-19 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
35–480（446 aa）
Fragment：core protein 1
|
Not recorded | HEC HEME C × 6 UQ2 UBIQUINONE-2 × 2 OST METHYL (2Z)-3-METHOXY-2-{2-[(E)-2-PHENYLVINYL]PHENYL}ACRYLATE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.2；277 K；20mM ammonium acetate, 20% glycerol, 12% PEG4000,
0.5M KCl, 0.1% diheptanoyl-phosphatidylcholine
, pH 7.2, VAPOR DIFFUSION, temperature 277K
|
Resolution 3.00 Å R-free 0.295 |
| 1SQV Crystal Structure Analysis of Bovine Bc1 with UHDBT Deposited 2004-03-19 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
34–480（447 aa）
|
Not recorded | HEC HEME C × 6 UHD 6-HYDROXY-5-UNDECYL-1,3-BENZOTHIAZOLE-4,7-DIONE × 2 UQ2 UBIQUINONE-2 × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.2；277 K；20mM ammonium acetate, 20% glycerol, 12% PEG4000,
0.5M KCl, 0.1% diheptanoyl-phosphatidylcholine, pH 7.2, VAPOR DIFFUSION, temperature 277K
|
Resolution 2.85 Å R-free 0.285 |
| 1SQX Crystal Structure Analysis of Bovine Bc1 with Stigmatellin A Deposited 2004-03-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
35–480（446 aa）
Fragment：core protein 1
|
Not recorded | UQ2 UBIQUINONE-2 × 2 HEC HEME C × 6 SMA STIGMATELLIN A × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.2；277 K；20mM ammonium acetate, 20% glycerol, 12% PEG4000,
0.5M KCl, 0.1% diheptanoyl-phosphatidylcholine
, pH 7.2, VAPOR DIFFUSION, temperature 277K
|
Resolution 2.60 Å R-free 0.281 |
| 2A06 Bovine cytochrome bc1 complex with stigmatellin bound Deposited 2005-06-16 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric |
Chain A
35–480（446 aa）
Chain N
35–480（446 aa）
|
Not recorded | JZR hexyl beta-D-glucopyranoside × 6 PO4 PHOSPHATE ION × 11 AZI AZIDE ION × 4 UNL UNKNOWN LIGAND × 74 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 SMA STIGMATELLIN A × 2 UQ Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer × 2 CDL CARDIOLIPIN × 4 GOL GLYCEROL × 7 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.65；277 K；PEG-3350, JEFFAMINE, GLYCEROL, CACODYLATE, HEXYLGLUCOSIDE , pH 6.65, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.10 Å R-free 0.258 |
| 2FYU Crystal structure of bovine heart mitochondrial bc1 with jg144 inhibitor Deposited 2006-02-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
35–480（446 aa）
Fragment：Core1
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 6 FDN (5S)-3-ANILINO-5-(2,4-DIFLUOROPHENYL)-5-METHYL-1,3-OXAZOLIDINE-2,4-DIONE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；277.2 K；50 mM MOPS 7.2pH, 20 mM Ammonium acetate, 20% glycerol.Incubation with 2-5 mol excess of JG144. Precipitant 12% PEG4000, 0.5 M KCl, 0.1% DHPC; Protein:PPT ratio 1:0.57, VAPOR DIFFUSION, SITTING DROP, temperature 277.2K
|
Resolution 2.26 Å R-free 0.283 |
| 2YBB Fitted model for bovine mitochondrial supercomplex I1III2IV1 by single particle cryo-EM (EMD-1876) Deposited 2011-03-02 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric |
Chain A
35–480（446 aa）
Chain a
35–480（446 aa）
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 7 FES FE2/S2 (INORGANIC) CLUSTER × 4 CA CALCIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 5 SMA STIGMATELLIN A × 2 UQ1 UBIQUINONE-1 × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 4 HEA HEME-A × 2 CU COPPER (II) ION × 3 ZN ZINC ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
10 MM KCL, 15 MM HEPES, RESIDUAL TREHALOSE；pH 7.7；10 MM KCL, 15 MM HEPES, RESIDUAL TREHALOSE
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 70, TEMPERATURE- 95, INSTRUMENT- CUSTOM-MADE HAND PLUNGER, METHOD- ONE- SIDED BLOTTING FOR 26 - 30 SECONDS BEFORE PLUNGING, DETAILS- SAMPLE WAS ADSORBED TO CARBON FILM FOR 30 SECONDS BEFORE BLOTTING
|
Resolution 19.00 Å |
| 3BCC STIGMATELLIN AND ANTIMYCIN BOUND CYTOCHROME BC1 COMPLEX FROM CHICKEN Deposited 1998-03-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: 20-MERIC |
Chain A
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 6 SIG STIGMATELLIN × 2 AMY ANTIMYCIN × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.7；20MM KMES PH6.7, 75MM NACL, 10% GLYCEROL, AND 6% PEG4000, INHIBITOR WAS ADDED FROM ETHANOLIC SOLUTION
|
Resolution 3.70 Å R-free 0.321 |
| 4D6T Cytochrome bc1 bound to the 4(1H)-pyridone GW844520 Deposited 2014-11-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
1–480（480 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 2 4X9 3-chloro-2,6-dimethyl-5-{4-[4-(trifluoromethoxy)phenoxy]phenyl}pyridin-4-ol × 1 PO4 PHOSPHATE ION × 6 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 HEC HEME C × 1 CDL CARDIOLIPIN × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.8；50 MM POTASSIUM PHOSPHATE BUFFER PH 6.8, 12% P4000, 1.4% HECAMEG
|
Resolution 3.57 Å R-free 0.252 |
| 4D6T Cytochrome bc1 bound to the 4(1H)-pyridone GW844520 Deposited 2014-11-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain N
1–480（480 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 2 4X9 3-chloro-2,6-dimethyl-5-{4-[4-(trifluoromethoxy)phenoxy]phenyl}pyridin-4-ol × 1 PO4 PHOSPHATE ION × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 HEC HEME C × 1 CDL CARDIOLIPIN × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.8；50 MM POTASSIUM PHOSPHATE BUFFER PH 6.8, 12% P4000, 1.4% HECAMEG
|
Resolution 3.57 Å R-free 0.252 |
| 4D6U Cytochrome bc1 bound to the 4(1H)-pyridone GSK932121 Deposited 2014-11-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
1–480（480 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 2 G8U 3-chloro-6-(hydroxymethyl)-2-methyl-5-{4-[3-(trifluoromethoxy)phenoxy]phenyl}pyridin-4-ol × 1 PO4 PHOSPHATE ION × 6 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 HEC HEME C × 1 CDL CARDIOLIPIN × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.8；SITTING DROP VAPOUR DIFFUSION 50 MM KPI, PH 6.8, 100 MM NACL, 3 MM NAN3, 10 - 13 % PEG4000,1.4% HECAMEG
|
Resolution 4.09 Å R-free 0.271 |
| 4D6U Cytochrome bc1 bound to the 4(1H)-pyridone GSK932121 Deposited 2014-11-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain N
1–480（480 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 2 G8U 3-chloro-6-(hydroxymethyl)-2-methyl-5-{4-[3-(trifluoromethoxy)phenoxy]phenyl}pyridin-4-ol × 1 PO4 PHOSPHATE ION × 3 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 HEC HEME C × 1 CDL CARDIOLIPIN × 2 FES FE2/S2 (INORGANIC) CLUSTER × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.8；SITTING DROP VAPOUR DIFFUSION 50 MM KPI, PH 6.8, 100 MM NACL, 3 MM NAN3, 10 - 13 % PEG4000,1.4% HECAMEG
|
Resolution 4.09 Å R-free 0.271 |
| 5GPN Architecture of mammalian respirasome Deposited 2016-08-03 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 84 PDB declaration: 84-meric |
Chain A
35–480（446 aa）
Chain M
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 4 SF4 IRON/SULFUR CLUSTER × 6 FMN FLAVIN MONONUCLEOTIDE × 1 NDP NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 ZN ZINC ION × 1 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen NITROGEN
|
Resolution 5.40 Å |
| 5KLV Structure of bos taurus cytochrome bc1 with fenamidone inhibited Deposited 2016-06-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
35–480（446 aa）
|
Not recorded | 6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 4 CDL CARDIOLIPIN × 6 GOL GLYCEROL × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 FNM (5S)-5-methyl-2-(methylsulfanyl)-5-phenyl-3-(phenylamino)-3,5-dihydro-4H-imidazol-4-one × 2 8PE (2R)-3-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(tetradecanoyloxy)propyl octadecanoate × 2 CL CHLORIDE ION × 2 HEC HEME C × 2 PEF DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；277 K；50 mM MOPS 7.2 pH, 20 mM Ammonium acetate, 20% glycerol. Incubation with 2-5 mol excess of Fenamidone. Precipitant 12% PEG4000, 0.5 M KCl, 0.1% DHPC; Protein:PPT ratio 1:0.57
|
Resolution 2.65 Å R-free 0.269 |
| 5LUF Cryo-EM of bovine respirasome Deposited 2016-09-08 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 62 PDB declaration: 62-meric |
Chain c
35–480（446 aa）
Chain l
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 HEA HEME-A × 2 ZN ZINC ION × 1 SF4 IRON/SULFUR CLUSTER × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.7
cryo-EM vitrification conditions
Cryogen ETHANE；10 s blotting
|
Resolution 9.10 Å |
| 5OKD Crystal structure of bovine Cytochrome bc1 in complex with inhibitor SCR0911. Deposited 2017-07-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
1–480（480 aa）
|
Not recorded | PG4 TETRAETHYLENE GLYCOL × 3 6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 1 CDL CARDIOLIPIN × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 9XE 7-methoxy-3-methyl-2-[5-[4-(trifluoromethyloxy)phenyl]pyridin-3-yl]-1~{H}-quinolin-4-one × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 HEC HEME C × 1 PO4 PHOSPHATE ION × 6 FES FE2/S2 (INORGANIC) CLUSTER × 1 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；Protein 40mg/mL with 1.6% HECAMEG; reservoir solution 50mM KPi pH 6.8, 100mM NaCl, 3mM NaN3, 10-13% PEG4000
|
Resolution 3.10 Å R-free 0.240 |
| 6FO0 CryoEM structure of bovine cytochrome bc1 in complex with the anti-malarial compound GSK932121 Deposited 2018-02-05 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric |
Chain A
1–480（480 aa）
Chain N
1–480（480 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 4 G8U 3-chloro-6-(hydroxymethyl)-2-methyl-5-{4-[3-(trifluoromethoxy)phenoxy]phenyl}pyridin-4-ol × 2 HEC HEME C × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；blot for 6 seconds with a blot force of 6 before plunge-freezing
|
Resolution 4.10 Å |
| 6FO2 CryoEM structure of bovine cytochrome bc1 with no ligand bound Deposited 2018-02-05 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric |
Chain A
1–480（480 aa）
Chain N
1–480（480 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；blot for 6 seconds with a blot force of 6 before plunge-freezing
|
Resolution 4.40 Å |
| 6FO6 CryoEM structure of bovine cytochrome bc1 in complex with the anti-malarial inhibitor SCR0911 Deposited 2018-02-06 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric |
Chain A
1–480（480 aa）
Chain N
1–480（480 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 4 DY2 7-methoxy-3-methyl-2-[5-[4-(trifluoromethyloxy)phenyl]pyridin-3-yl]quinolin-4-ol × 2 HEC HEME C × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；blot for 6 seconds with a blot force of 6 before plunge-freezing
|
Resolution 4.10 Å |
| 6HAW Crystal structure of bovine cytochrome bc1 in complex with 2-pyrazolyl quinolone inhibitor WDH2G7 Deposited 2018-08-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
36–479（444 aa）
|
Not recorded | 6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 1 PGE TRIETHYLENE GLYCOL × 4 PO4 PHOSPHATE ION × 11 CDL CARDIOLIPIN × 4 PG4 TETRAETHYLENE GLYCOL × 1 PX6 1,2-DIPALMITOYL-SN-GLYCERO-3-PHOSPHATE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 FX2 7-methoxy-3-methyl-2-[1-[[4-(trifluoromethyloxy)phenyl]methyl]pyrazol-4-yl]-3~{H}-quinolin-4-one × 1 HEC HEME C × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；Protein 40mg/mL with 1.6% HECAMEG; reservoir solution 50mM KPi pH 6.8, 100mM NaCl, 3mM NaN3, 10-12% PEG4000.
|
Resolution 3.45 Å R-free 0.242 |
| 6NHG Rhodobacter sphaeroides Mitochondrial respiratory chain complex Deposited 2018-12-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
35–480（446 aa）
|
Not recorded | 6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 4 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 AZO METHYL (2Z)-2-(2-{[6-(2-CYANOPHENOXY)PYRIMIDIN-4-YL]OXY}PHENYL)-3-METHOXYACRYLATE × 2 8PE (2R)-3-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(tetradecanoyloxy)propyl octadecanoate × 2 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 MC3 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；277 K；50 mM MOPS 7.2 pH, 20 mM Ammonium acetate, 20% glycerol. Incubation with 2-5 mol excess of Azoxystrobin. Precipitant 12% PEG4000, 0.5 M KCl, 0.1% DHPC; Protein:PPT ratio 1:0.57
|
Resolution 2.80 Å R-free 0.289 |
| 6XVF Crystal structure of bovine cytochrome bc1 in complex with tetrahydro-quinolone inhibitor JAG021 Deposited 2020-01-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric |
Chain A
36–480（445 aa）
|
Not recorded | PG4 TETRAETHYLENE GLYCOL × 6 6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 2 PO4 PHOSPHATE ION × 18 CDL CARDIOLIPIN × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 LMT DODECYL-BETA-D-MALTOSIDE × 2 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 4 JAG 2-methyl-3-[4-[4-(trifluoromethyloxy)phenoxy]phenyl]-5,6,7,8-tetrahydro-3~{H}-quinolin-4-one × 2 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；Protein 40mg/mL with 1.6% HECAMEG;
Reservoir solution: 50mM KPi pH 6.8, 100mM NaCl, 3mM NaN3, 10-13% PEG4000
|
Resolution 3.50 Å R-free 0.232 |
| 6ZFS Crystal structure of bovine cytochrome bc1 in complex with quinolone inhibitor WDH-1U-4 Deposited 2020-06-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
36–479（444 aa）
|
Not recorded | 6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 1 PO4 PHOSPHATE ION × 7 CDL CARDIOLIPIN × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 PG4 TETRAETHYLENE GLYCOL × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 JGW 2-methyl-3-[4-[[4-(trifluoromethyloxy)phenyl]methyl]phenyl]-3~{H}-quinolin-4-one × 1 HEC HEME C × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；Protein 40mg/mL with 1.6% HECAMEG; reservoir solution 50mM KPi pH 6.8, 100mM NaCl, 3mM NaN3, 10-13% PEG4000
|
Resolution 3.50 Å R-free 0.246 |
| 6ZFT Crystal structure of bovine cytochrome bc1 in complex with quinolone inhibitor CK-2-68 Deposited 2020-06-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
37–480（444 aa）
|
Not recorded | PG4 TETRAETHYLENE GLYCOL × 4 6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 1 CDL CARDIOLIPIN × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 JHB 7-chloranyl-3-methyl-2-[4-[[4-(trifluoromethyloxy)phenyl]methyl]phenyl]-1~{H}-quinolin-4-one × 1 LMT DODECYL-BETA-D-MALTOSIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 PO4 PHOSPHATE ION × 6 HEC HEME C × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；Protein 40mg/mL with 1.6% HECAMEG; reservoir solution 50mM KPi pH 6.8, 100mM NaCl, 3mM NaN3, 10-13% PEG4000
|
Resolution 3.30 Å R-free 0.244 |
| 6ZFU Crystal structure of bovine cytochrome bc1 in complex with quinolone inhibitor RKA066 Deposited 2020-06-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
36–479（444 aa）
|
Not recorded | PG4 TETRAETHYLENE GLYCOL × 3 6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 1 CDL CARDIOLIPIN × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 JHE 3-methyl-1-oxidanyl-2-[4-[[4-(trifluoromethyloxy)phenyl]methyl]phenyl]quinolin-4-one × 1 HEC HEME C × 1 PO4 PHOSPHATE ION × 5 FES FE2/S2 (INORGANIC) CLUSTER × 1 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；Protein 40mg/mL with 1.6% HECAMEG; reservoir solution 50mM KPi pH 6.8, 100mM NaCl, 3mM NaN3, 10-13% PEG4000
|
Resolution 3.50 Å R-free 0.238 |
| 7DGQ Activity optimized supercomplex state1 Deposited 2020-11-12 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric |
Chain k
1–480（480 aa）
Chain w
1–480（480 aa）
|
Not recorded | PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 CU COPPER (II) ION × 3 HEA HEME-A × 2 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 UQ2 UBIQUINONE-2 × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.00 Å |
| 7DGR Activity optimized supercomplex state2 Deposited 2020-11-12 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric |
Chain k
1–480（480 aa）
Chain w
1–480（480 aa）
|
Not recorded | FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 CDL CARDIOLIPIN × 3 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å |
| 7DGS Activity optimized supercomplex state3 Deposited 2020-11-12 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric |
Chain k
1–480（480 aa）
Chain w
1–480（480 aa）
|
Not recorded | FES FE2/S2 (INORGANIC) CLUSTER × 3 CDL CARDIOLIPIN × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 5 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.80 Å |
| 7DKF Activity optimized supercomplex state4 Deposited 2020-11-24 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 80 PDB declaration: 80-meric |
Chain A1
1–480（480 aa）
Chain M1
1–480（480 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 3 CDL CARDIOLIPIN × 1 FMN FLAVIN MONONUCLEOTIDE × 1 SF4 IRON/SULFUR CLUSTER × 6 ZN ZINC ION × 2 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 HEA HEME-A × 2 CU COPPER (II) ION × 3 MG MAGNESIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.30 Å |
| 7R3V Crystal structure of bovine Cytochrome bc1 in complex with inhibitor CK-2-67. Deposited 2022-02-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
36–480（445 aa）
|
Not recorded | PG4 TETRAETHYLENE GLYCOL × 3 6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 1 CDL CARDIOLIPIN × 3 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 2 I2Q 3-methyl-2-[4-[[4-(trifluoromethoxy)phenyl]methyl]phenyl]-1H-quinolin-4-one × 1 DMS DIMETHYL SULFOXIDE × 1 PO4 PHOSPHATE ION × 9 HEC HEME C × 1 FES FE2/S2 (INORGANIC) CLUSTER × 1 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；Protein 40mg/mL with 1.6% HECAMEG; reservoir solution 50mM KPi pH 6.8, 100mM NaCl, 3mM NaN3, 9-12% PEG4000
|
Resolution 3.20 Å R-free 0.256 |
| 7TAY Bos Taurus Mitochondrial BC1 in complex with Pyramoxadone Deposited 2021-12-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
35–480（446 aa）
|
Not recorded | 6PE 1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE × 4 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 PQU (5S)-3-anilino-5-methyl-5-(6-phenoxypyridin-3-yl)-1,3-oxazolidine-2,4-dione × 2 8PE (2R)-3-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(tetradecanoyloxy)propyl octadecanoate × 2 PEF DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE × 2 CL CHLORIDE ION × 2 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PX4 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；50 mM MOPS 7.2 pH, 20 mM Ammonium acetate, 20% glycerol. Incubation with 5 molar excess of pyramoxadone. Precipitant 12% PEG4000, 0.5 M KCL, 0.1% DHPC.
|
Resolution 2.95 Å R-free 0.258 |
| 7TZ6 Structure of mitochondrial bc1 in complex with ck-2-68 Deposited 2022-02-15 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
35–480（446 aa）
Chain N
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 4 JHB 7-chloranyl-3-methyl-2-[4-[[4-(trifluoromethyloxy)phenyl]methyl]phenyl]-1~{H}-quinolin-4-one × 2 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.88 Å |
| 8P65 Cytochrome bc1 complex (Bos taurus) Deposited 2023-05-25 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
35–480（446 aa）
Chain N
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot time of 4s with blot force of -3
|
Resolution 3.00 Å |
| 9GCX Crystal structure of bovine Cytochrome bc1 in complex with inhibitor F8 Deposited 2024-08-03 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
1–480（480 aa）
|
Not recorded | LOP (1R)-2-{[(R)-(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(DODECANOYLOXY)METHYL]ETHYL (9Z)-OCTADEC-9-ENOATE × 12 CDL CARDIOLIPIN × 6 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 A1IKG 2-methyl-3-(4-(4-(trifluoromethoxy)phenoxy)phenyl)-1,5,7,8-tetrahydro-4H-pyrano[4,3-b]pyridin-4-one × 2 LMT DODECYL-BETA-D-MALTOSIDE × 2 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 PSC (7R,17E,20E)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSA-17,20-DIEN-1-AMINIUM 4-OXIDE × 2 SO4 SULFATE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；277 K；50 mM KPi pH 6.8, 100 mM NaCl, 3 mM NaN3, 10-20% PEG4000
|
Resolution 3.52 Å R-free 0.241 |
| 9W2X Cryo-EM structure of complex III on the bovine heart submitochondrial particles, III-1 Deposited 2025-07-28 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
35–480（446 aa）
Chain N
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å |
| 9W2Y Cryo-EM structure of complex III on the bovine heart submitochondrial particles, III-2 Deposited 2025-07-28 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric |
Chain A
35–480（446 aa）
Chain L
35–480（446 aa）
|
Not recorded | HEM PROTOPORPHYRIN IX CONTAINING FE × 4 HEC HEME C × 2 FES FE2/S2 (INORGANIC) CLUSTER × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
46 other PDB entries and 51 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | QCR1_BOVIN |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–444； UniProt 36–479 Author chain N； PDBConstruct 1–444； UniProt 36–479 |
