Current Protein Identity：P12883
New Search
Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2FXM Structure of the human beta-myosin S2 fragment Deposited 2006-02-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
838–963（126 aa）
Fragment：DELTA-S2 FRAGMENT (838-963)
Chain B
838–963（126 aa）
Fragment：DELTA-S2 FRAGMENT (838-963)
|
Not recorded | HG MERCURY (II) ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；296 K；PEG 3350, LITHIUM CITRATE, TRIS- HCL, pH 8.50, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 296K
|
Resolution 2.70 Å R-free 0.283 |
| 2FXO Structure of the human beta-myosin S2 fragment Deposited 2006-02-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
838–963（126 aa）
Fragment：DELTA-S2 FRAGMENT (838-963)
Chain B
838–963（126 aa）
Fragment：DELTA-S2 FRAGMENT (838-963)
|
Mutation：E924K Mutation：E924K | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；296 K；PEG 3350, SODIUM ACETATE, TRIS-HCL, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 296K
|
Resolution 2.50 Å R-free 0.349 |
| 2FXO Structure of the human beta-myosin S2 fragment Deposited 2006-02-06 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
838–963（126 aa）
Fragment：DELTA-S2 FRAGMENT (838-963)
Chain D
838–963（126 aa）
Fragment：DELTA-S2 FRAGMENT (838-963)
|
Mutation：E924K Mutation：E924K | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；296 K；PEG 3350, SODIUM ACETATE, TRIS-HCL, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 296K
|
Resolution 2.50 Å R-free 0.349 |
| 3DTP Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP Deposited 2008-07-15 | Assembly 1 Insufficient information Heteromer；Protein × 216 PDB declaration: 216-meric（216） Consistent with protein count |
Chain A
842–961（120 aa）
Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972)
Chain B
842–963（122 aa）
Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3；pH 7；100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3
cryo-EM vitrification conditions
Plunging in a liquid ethane.
Blotting was performed from one side of the grid till a thin sample film on it using Whatman
No 42 filter paper, then the grid was immediately plunged under gravity into liquid ethane cooled
by liquid nitrogen.
Grids were stored under liquid nitrogen.
|
Resolution 20.00 Å |
| 3DTP Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP Deposited 2008-07-15 | Assembly 2 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
842–961（120 aa）
Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972)
Chain B
842–963（122 aa）
Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3；pH 7；100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3
cryo-EM vitrification conditions
Plunging in a liquid ethane.
Blotting was performed from one side of the grid till a thin sample film on it using Whatman
No 42 filter paper, then the grid was immediately plunged under gravity into liquid ethane cooled
by liquid nitrogen.
Grids were stored under liquid nitrogen.
|
Resolution 20.00 Å |
| 3DTP Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP Deposited 2008-07-15 | Assembly 3 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
842–961（120 aa）
Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972)
Chain B
842–963（122 aa）
Fragment：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3；pH 7；100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3
cryo-EM vitrification conditions
Plunging in a liquid ethane.
Blotting was performed from one side of the grid till a thin sample film on it using Whatman
No 42 filter paper, then the grid was immediately plunged under gravity into liquid ethane cooled
by liquid nitrogen.
Grids were stored under liquid nitrogen.
|
Resolution 20.00 Å |
| 4DB1 Cardiac human myosin S1dC, beta isoform complexed with Mn-AMPPNP Deposited 2012-01-13 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
2–783（782 aa）
Fragment：UNP residues 2-783
|
Not recorded | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MN MANGANESE (II) ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；precipitant: 7.5% PEG 8000, 0.05M MES, 0.05M acetate, 0.25M sodium chloride, 0.01M manganese chloride, 0.1% sodium cholate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.60 Å R-free 0.257 |
| 4DB1 Cardiac human myosin S1dC, beta isoform complexed with Mn-AMPPNP Deposited 2012-01-13 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
2–783（782 aa）
Fragment：UNP residues 2-783
|
Not recorded | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MN MANGANESE (II) ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；precipitant: 7.5% PEG 8000, 0.05M MES, 0.05M acetate, 0.25M sodium chloride, 0.01M manganese chloride, 0.1% sodium cholate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.60 Å R-free 0.257 |
| 4DB1 Cardiac human myosin S1dC, beta isoform complexed with Mn-AMPPNP Deposited 2012-01-13 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–783（782 aa）
Fragment：UNP residues 2-783
Chain B
2–783（782 aa）
Fragment：UNP residues 2-783
|
Not recorded | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 MN MANGANESE (II) ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；precipitant: 7.5% PEG 8000, 0.05M MES, 0.05M acetate, 0.25M sodium chloride, 0.01M manganese chloride, 0.1% sodium cholate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.60 Å R-free 0.257 |
| 4P7H Structure of Human beta-Cardiac Myosin Motor Domain::GFP chimera Deposited 2014-03-27 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–787（787 aa）
Fragment：UNP P12883 residues 1-787,UNP P42212 residues 5-238
|
Mutation：Q80R, K101N, V163A, I167T, S175G, D190N Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；10% Tacsimate, pH 6.0, 10% glycerol, 14-15% PEG 3350, 0.2 mM MgCL2, and 5 mM TCEP
|
Resolution 3.20 Å R-free 0.284 |
| 4P7H Structure of Human beta-Cardiac Myosin Motor Domain::GFP chimera Deposited 2014-03-27 | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–787（787 aa）
Fragment：UNP P12883 residues 1-787,UNP P42212 residues 5-238
|
Mutation：Q80R, K101N, V163A, I167T, S175G, D190N Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；10% Tacsimate, pH 6.0, 10% glycerol, 14-15% PEG 3350, 0.2 mM MgCL2, and 5 mM TCEP
|
Resolution 3.20 Å R-free 0.284 |
| 4PA0 Omecamtiv Mercarbil binding site on the Human Beta-Cardiac Myosin Motor Domain Deposited 2014-04-06 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–787（787 aa）
Fragment：UNP P12883 residues 1-787, UNP P42212 residues 5-234
|
Mutation：Q80R, V163A, I167T, S175G, D190N Non-standard monomer：Yes (specific site not provided by mmCIF) | 2OW methyl 4-(2-fluoro-3-{[(6-methylpyridin-3-yl)carbamoyl]amino}benzyl)piperazine-1-carboxylate × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；295 K；Tacsimate, pH 6.0, PEG 3350, glycerol, MgCL2, TCEP and ligand, omecamptiv mercarbil
|
Resolution 2.25 Å R-free 0.246 |
| 4PA0 Omecamtiv Mercarbil binding site on the Human Beta-Cardiac Myosin Motor Domain Deposited 2014-04-06 | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–787（787 aa）
Fragment：UNP P12883 residues 1-787, UNP P42212 residues 5-234
|
Mutation：Q80R, V163A, I167T, S175G, D190N Non-standard monomer：Yes (specific site not provided by mmCIF) | 2OW methyl 4-(2-fluoro-3-{[(6-methylpyridin-3-yl)carbamoyl]amino}benzyl)piperazine-1-carboxylate × 1 GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；295 K；Tacsimate, pH 6.0, PEG 3350, glycerol, MgCL2, TCEP and ligand, omecamptiv mercarbil
|
Resolution 2.25 Å R-free 0.246 |
| 4XA1 Crystal Structure of the coiled-coil surrounding Skip 1 of MYH7 Deposited 2014-12-12 | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1173–1238（66 aa）
Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
Chain B
1173–1238（66 aa）
Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；20% (w/v) polyethylene glycol methyl ether 2000, 20 mM SrCl2, 100 mM HEPES pH 7.6, 5% pentaerythritol ethoxylate (17/8 PO/OH) 797, 0.5% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)
|
Resolution 3.20 Å R-free 0.285 |
| 4XA1 Crystal Structure of the coiled-coil surrounding Skip 1 of MYH7 Deposited 2014-12-12 | Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1173–1238（66 aa）
Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
Chain D
1173–1238（66 aa）
Fragment：UNP P13848 residues 1-49,UNP Q12883 residues 1173-1238,UNP Q15691 residues 211-251
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；298 K；20% (w/v) polyethylene glycol methyl ether 2000, 20 mM SrCl2, 100 mM HEPES pH 7.6, 5% pentaerythritol ethoxylate (17/8 PO/OH) 797, 0.5% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)
|
Resolution 3.20 Å R-free 0.285 |
| 4XA3 Crystal structure of the coiled-coil surrounding Skip 2 of MYH7 Deposited 2014-12-12 | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1361–1425（65 aa）
Fragment：UNP P13848 residues 1-49,UNP P12883 residues 1361-1425,UNP Q15691 residues 215-251
Chain B
1361–1425（65 aa）
Fragment：UNP P13848 residues 1-49,UNP P12883 residues 1361-1425,UNP Q15691 residues 215-251
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；4.5% (w/v) polyethylene glycol 8000, 100 mM sodium acetate pH 5.0, 50 mM CaCl2, 2.5% (w/v) 3-methoxy-3-methyl-1-butanol
|
Resolution 2.55 Å R-free 0.311 |
| 4XA4 Crystal Structure of the coiled-coil surrounding Skip 3 of MYH7 Deposited 2014-12-12 | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1551–1609（59 aa）
Fragment：UNP Q13426 residues 2-147,UNP P12883 residues 1551-1609
Chain B
1551–1609（59 aa）
Fragment：UNP Q13426 residues 2-147,UNP P12883 residues 1551-1609
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；30% (w/v) polyethylene glycol 1500, 250 mM tetramethylammonium chloride, 100 mM 3-[4-(2-Hydroxyethyl)-1-piperazinyl]propanesulfonic acid (HEPPS)
|
Resolution 2.33 Å R-free 0.274 |
| 4XA6 Crystal Structure of the coiled-coil surrounding Skip 4 of MYH7 Deposited 2014-12-12 | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1777–1855（79 aa）
Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251
Chain B
1777–1855（79 aa）
Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.4；298 K；18% (w/v) polyethylene glycol 2000 methyl ether, 100 mM piperazine-N,N-bis(2-ethanesulfonic acid) (PIPES)
|
Resolution 3.42 Å R-free 0.297 |
| 4XA6 Crystal Structure of the coiled-coil surrounding Skip 4 of MYH7 Deposited 2014-12-12 | Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1777–1855（79 aa）
Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251
Chain D
1777–1855（79 aa）
Fragment：UNP P13848 residues 2-50,UNP P02564 residues 1777-1855,UNP Q15691 residues 209-251
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.4；298 K；18% (w/v) polyethylene glycol 2000 methyl ether, 100 mM piperazine-N,N-bis(2-ethanesulfonic acid) (PIPES)
|
Resolution 3.42 Å R-free 0.297 |
| 5CHX Crystal Structure of amino acids 1590-1657 of MYH7 Deposited 2015-07-10 | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1590–1657（68 aa）
Fragment：UNP Q13426 residues 2-143, UNP P12833 1590-1657
Chain B
1590–1657（68 aa）
Fragment：UNP Q13426 residues 2-143, UNP P12833 1590-1657
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；16% (w/v) MEPEG 2000, 250 mM potassium nitrate, 100 mM 3-(N-morpholino)propanesulfonic acid (MOPS)
|
Resolution 2.30 Å R-free 0.265 |
| 5CHX Crystal Structure of amino acids 1590-1657 of MYH7 Deposited 2015-07-10 | Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1590–1657（68 aa）
Fragment：UNP Q13426 residues 2-143, UNP P12833 1590-1657
Chain B
1590–1657（68 aa）
Fragment：UNP Q13426 residues 2-143, UNP P12833 1590-1657
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；16% (w/v) MEPEG 2000, 250 mM potassium nitrate, 100 mM 3-(N-morpholino)propanesulfonic acid (MOPS)
|
Resolution 2.30 Å R-free 0.265 |
| 5CJ0 Crystal Structure of Amino Acids 1631-1692 of MYH7 Deposited 2015-07-13 | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1631–1692（62 aa）
Fragment：UNP Q13426 residues 2-142, UNP P12883 residues 1631-1692
Chain B
1631–1692（62 aa）
Fragment：UNP Q13426 residues 2-142, UNP P12883 residues 1631-1692
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；14% (w/v) MEPEG 5000, 200 mM glycine, 100 mM bistrispropane pH 7.0.
|
Resolution 2.30 Å R-free 0.238 |
| 5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13 | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1526–1571（46 aa）
Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Chain B
1526–1571（46 aa）
Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5
|
Resolution 2.10 Å R-free 0.250 |
| 5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13 | Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1526–1571（46 aa）
Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Chain D
1526–1571（46 aa）
Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5
|
Resolution 2.10 Å R-free 0.250 |
| 5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13 | Assembly 3 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
1526–1571（46 aa）
Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Chain F
1526–1571（46 aa）
Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5
|
Resolution 2.10 Å R-free 0.250 |
| 5CJ1 Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7 Deposited 2015-07-13 | Assembly 4 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
1526–1571（46 aa）
Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
Chain H
1526–1571（46 aa）
Fragment：UNP P13848 residues 2-52, UNP P12833 residues 1526-1571
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；16% (w/v) PEG 8000, 400 mM malonate pH 7.2, and 100 mM triethanolamine pH 7.5
|
Resolution 2.10 Å R-free 0.250 |
| 5CJ4 Crystal Structure of Amino Acids 1562-1622 of MYH7 Deposited 2015-07-13 | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1562–1622（61 aa）
Fragment：UNP Q13426 residues 2-144, UNP P12883 1562-1622
Chain B
1562–1622（61 aa）
Fragment：UNP Q13426 residues 2-144, UNP P12883 1562-1622
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；23% (w/v) PEG 4000, 500 mM NaCl, 100 mM triethanolamine pH 8.0.
|
Resolution 3.10 Å R-free 0.280 |
| 5CJ4 Crystal Structure of Amino Acids 1562-1622 of MYH7 Deposited 2015-07-13 | Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1562–1622（61 aa）
Fragment：UNP Q13426 residues 2-144, UNP P12883 1562-1622
Chain D
1562–1622（61 aa）
Fragment：UNP Q13426 residues 2-144, UNP P12883 1562-1622
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；23% (w/v) PEG 4000, 500 mM NaCl, 100 mM triethanolamine pH 8.0.
|
Resolution 3.10 Å R-free 0.280 |
| 5TBY HUMAN BETA CARDIAC HEAVY MEROMYOSIN INTERACTING-HEADS MOTIF OBTAINED BY HOMOLOGY MODELING (USING SWISS-MODEL) OF HUMAN SEQUENCE FROM APHONOPELMA HOMOLOGY MODEL (PDB-3JBH), RIGIDLY FITTED TO HUMAN BETA-CARDIAC NEGATIVELY STAINED THICK FILAMENT 3D-RECONSTRUCTION (EMD-2240) Deposited 2016-09-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
1–1935（1935 aa）
Fragment：SUBFRAGMENT 1(S1)
Chain B
1–1935（1935 aa）
Fragment：SUBFRAGMENT 1(S1)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE；PLUNGING IN A LIQUID ETHANE COOLED BY LIQUID NITROGEN. BLOTTING WAS PERFORMED FROM ONE SIDE OF THE GRID TILL A THIN SAMPLE FILM ON IT USING WHATMAN NO. 42 FILTER PAPER, THEN THE GRID WAS IMMEDIATELY PLUNGED UNDER GRAVITY INTO LIQUID ETHANE COOLED BY LIQUID NITROGEN. GRIDS WERE STORED UNDER LIQUID NITROGEN.
|
Resolution 20.00 Å |
| 5WJ7 Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Xrcc4 Deposited 2017-07-21 | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1733–1797（65 aa）
Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1733-1797
Chain B
1733–1797（65 aa）
Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1733-1797
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；16% (w/v) Methyl-Ether PEG 5K, 300 mM glycine, 100 mM triethanolamine pH 7.5
|
Resolution 2.50 Å R-free 0.249 |
| 5WJB Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Gp7 Deposited 2017-07-21 | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1733–1797（65 aa）
Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797
Chain B
1733–1797（65 aa）
Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；14% (w/v) methyl-ether PEG 2K, 1.5%(w/v) myo-inositol, 100 mM HEPES pH 7.5, 50 mM magnesium chloride
|
Resolution 2.90 Å R-free 0.300 |
| 5WJB Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Gp7 Deposited 2017-07-21 | Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1733–1797（65 aa）
Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797
Chain D
1733–1797（65 aa）
Fragment：UNP P13848 residues 2-47, UNP P12833 residues 1733-1797
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；14% (w/v) methyl-ether PEG 2K, 1.5%(w/v) myo-inositol, 100 mM HEPES pH 7.5, 50 mM magnesium chloride
|
Resolution 2.90 Å R-free 0.300 |
| 5WLQ Crystal Structure of Amino Acids 1677-1755 of Human Beta Cardiac Myosin Fused to Gp7 and Eb1 Deposited 2017-07-27 | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1677–1755（79 aa）
Fragment：UNP P13848 residues 2-48 UNP Q15691 residues 208-256, UNP P12883 residues 1677-1755
|
Not recorded | SO4 SULFATE ION × 2 TMO trimethylamine oxide × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；1.6 M Ammonium Aulfate, 500 mM Trimethyl Ammonium N-Oxide, 100 mM Bis-tris Propane pH 9.0
|
Resolution 3.10 Å R-free 0.236 |
| 5WLZ Crystal Structure of Amino Acids 1677-1758 of Human Beta Cardiac Myosin Fused to Xrcc4 Deposited 2017-07-28 | Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1677–1758（82 aa）
Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1677-1758
Chain B
1677–1758（82 aa）
Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1677-1758
Chain C
1677–1758（82 aa）
Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1677-1758
Chain D
1677–1758（82 aa）
Fragment：UNP Q13426 residues 2-132, UNP P12883 residues 1677-1758
|
Mutation：E29K, E51K, D57A, D58T, E62N, C93R, E98K,C128D, C132A Mutation：E29K, E51K, D57A, D58T, E62N, C93R, E98K,C128D, C132A Mutation：E29K, E51K, D57A, D58T, E62N, C93R, E98K,C128D, C132A Mutation：E29K, E51K, D57A, D58T, E62N, C93R, E98K,C128D, C132A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；8-10% methyl-ether polyethylene glycol (MEPEG) 5K, 300 mM glycine, bis-tris propane pH 7.0, 1.5-3.0% (w/v) jeffamine M-600
|
Resolution 3.50 Å R-free 0.249 |
| 5WME Crystal Structure of Amino Acids 1729-1786 of Human Beta Cardiac Myosin Fused to Gp7 as Anti-Parallel Four-Helix Bundle Deposited 2017-07-28 | Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1728–1786（59 aa）
Fragment：UNP P13848 residues 2-48, UNP P12883 residues 1729-1786
Chain B
1728–1786（59 aa）
Fragment：UNP P13848 residues 2-48, UNP P12883 residues 1729-1786
Chain C
1728–1786（59 aa）
Fragment：UNP P13848 residues 2-48, UNP P12883 residues 1729-1786
Chain D
1728–1786（59 aa）
Fragment：UNP P13848 residues 2-48, UNP P12883 residues 1729-1786
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；18% (w/v) pentaerythritol ethoxylate 797, 150 mM ammonium thiocyanate, 100 mM sodium acetate pH 5.0.
|
Resolution 2.30 Å R-free 0.264 |
| 8ACT structure of the human beta-cardiac myosin folded-back off state Deposited 2022-07-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
3–906（904 aa）
Chain B
3–906（904 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8EFD Human cardiac myosin II and associated essential light chain in the rigor conformation Deposited 2022-09-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–842（842 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8EFE Human beta-cardiac myosin II bound to ADP-MG2+ and the associated essential light chain Deposited 2022-09-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–842（842 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8EFH Helical reconstruction of the human cardiac actin-tropomyosin-myosin complex in complex with ADP-Mg2+ Deposited 2022-09-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–842（842 aa）
|
Not recorded | MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8EFI Helical reconstruction of the human cardiac actin-tropomyosin-myosin complex in the rigor form Deposited 2022-09-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain M
1–1935（1935 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8ENC Helical reconstruction of the human cardiac actin-tropomyosin-myosin loop 4 7G mutant complex Deposited 2022-09-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain M
1–1935（1935 aa）
|
Mutation：Residues 366-372 substituted with seven glycines | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8G4L Cryo-EM structure of the human cardiac myosin filament Deposited 2023-02-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 123 PDB declaration: 123-meric（123） Consistent with protein count |
Chain A
1–1935（1935 aa）
Chain AA
1–1935（1935 aa）
Chain AB
1–1935（1935 aa）
Chain AG
1–1935（1935 aa）
Chain AH
1–1935（1935 aa）
Chain AI
1–1935（1935 aa）
Chain AJ
1–1935（1935 aa）
Chain AK
1–1935（1935 aa）
Chain AL
1–1935（1935 aa）
Chain AM
1–1935（1935 aa）
Chain AN
1–1935（1935 aa）
Chain AO
1–1935（1935 aa）
Chain AP
1–1935（1935 aa）
Chain AQ
1–1935（1935 aa）
Chain AR
1–1935（1935 aa）
Chain AS
1–1935（1935 aa）
Chain AT
1–1935（1935 aa）
Chain AU
1–1935（1935 aa）
Chain AV
1–1935（1935 aa）
Chain AW
1–1935（1935 aa）
Chain AX
1–1935（1935 aa）
Chain AY
1–1935（1935 aa）
Chain AZ
1–1935（1935 aa）
Chain B
1–1935（1935 aa）
Chain BA
1–1935（1935 aa）
Chain BB
1–1935（1935 aa）
Chain BG
1–1935（1935 aa）
Chain BH
1–1935（1935 aa）
Chain BI
1–1935（1935 aa）
Chain BJ
1–1935（1935 aa）
Chain BK
1–1935（1935 aa）
Chain BL
1–1935（1935 aa）
Chain BM
1–1935（1935 aa）
Chain BN
1–1935（1935 aa）
Chain BO
1–1935（1935 aa）
Chain BP
1–1935（1935 aa）
Chain BQ
1–1935（1935 aa）
Chain BR
1–1935（1935 aa）
Chain BS
1–1935（1935 aa）
Chain BT
1–1935（1935 aa）
Chain BU
1–1935（1935 aa）
Chain BV
1–1935（1935 aa）
Chain BW
1–1935（1935 aa）
Chain BX
1–1935（1935 aa）
Chain BY
1–1935（1935 aa）
Chain BZ
1–1935（1935 aa）
Chain G
1–1935（1935 aa）
Chain H
1–1935（1935 aa）
Chain I
1–1935（1935 aa）
Chain J
1–1935（1935 aa）
Chain K
1–1935（1935 aa）
Chain L
1–1935（1935 aa）
Chain M
1–1935（1935 aa）
Chain N
1–1935（1935 aa）
Chain O
1–1935（1935 aa）
Chain P
1–1935（1935 aa）
Chain Q
1–1935（1935 aa）
Chain R
1–1935（1935 aa）
Chain S
1–1935（1935 aa）
Chain T
1–1935（1935 aa）
Chain U
1–1935（1935 aa）
Chain V
1–1935（1935 aa）
Chain W
1–1935（1935 aa）
Chain X
1–1935（1935 aa）
Chain Y
1–1935（1935 aa）
Chain Z
1–1935（1935 aa）
Chain ae
1–1935（1935 aa）
Chain af
1–1935（1935 aa）
Chain ak
1–1935（1935 aa）
Chain al
1–1935（1935 aa）
Chain be
1–1935（1935 aa）
Chain bf
1–1935（1935 aa）
Chain bk
1–1935（1935 aa）
Chain bl
1–1935（1935 aa）
Chain e
1–1935（1935 aa）
Chain f
1–1935（1935 aa）
Chain k
1–1935（1935 aa）
Chain l
1–1935（1935 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.40 Å |
| 8ZB7 Human left ventricle ATM complex Deposited 2024-04-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric（16） Consistent with protein count |
Chain G
6–781（776 aa）
Chain H
6–781（776 aa）
Chain I
6–781（776 aa）
Chain J
6–781（776 aa）
Chain K
6–781（776 aa）
Chain M
6–781（776 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.19 Å |
| 8ZI9 Human left ventricle actin and myosin complex Deposited 2024-05-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain M
6–781（776 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.08 Å |
| 9GZ1 Beta-cardiac myosin interacting heads motif complexed to mavacamten Deposited 2024-10-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
2–1138（1137 aa）
Chain B
2–1138（1137 aa）
|
Not recorded | MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 XB2 Mavacamten × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 9GZ2 Beta-cardiac heavy meromyosin motor domain in the primed state complexed to mavacamten Deposited 2024-10-03 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
2–1138（1137 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 XB2 Mavacamten × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9GZ3 Beta-cardiac heavy meromyosin motor domain in the primed state Deposited 2024-10-03 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
2–1138（1137 aa）
|
Not recorded | MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9HTF Beta-cardiac myosin Y115H mutant motor domain in the pre-powerstroke state, MgADP.VO4 form Deposited 2024-12-19 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–810（810 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 VO4 VANADATE ION × 1 GOL GLYCEROL × 1 EDO 1,2-ETHANEDIOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；23 % PEG 3350 w:v, 0.3 M lithium sulfate, 0.1M Tris-HCl, 2 mM Mg.ADP.Vanadate
|
Resolution 2.48 Å R-free 0.255 |
| 9HTG Beta-cardiac myosin E497D mutant motor domain in the pre-powerstroke state, MgADP.VO4 form Deposited 2024-12-19 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–808（808 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 VO4 VANADATE ION × 1 GOL GLYCEROL × 1 EDO 1,2-ETHANEDIOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；22 % PEG 3350 w:v, 0.3 M lithium sulfate, 0.1M Tris-HCl, 2 mM Mg.ADP.Vanadate
|
Resolution 2.60 Å R-free 0.248 |
| 9I8P Human beta-cardiac myosin wild type motor domain in the pre-powerstroke state, MgADP.VO4 form Deposited 2025-02-05 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–808（808 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 VO4 VANADATE ION × 1 EDO 1,2-ETHANEDIOL × 2 SO4 SULFATE ION × 2 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；25 % PEG 3350 w:v, 0.25 M lithium sulfate, 0.1M Tris-HCl, 2 mM Mg.ADP.Vanadate. Optimal crystals were obtained using the micro-seeding technique.
|
Resolution 2.60 Å R-free 0.245 |
| 9YOP Cryo-EM structure of human beta-cardiac myosin in the interacting-heads motif and S2-FH docked state Deposited 2025-10-13 | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
1–1016（1016 aa）
Chain B
1–1016（1016 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 9YP4 Cryo-EM structure of human beta-cardiac myosin bound to omecamtiv mecarbil in the interacting-heads motif and S2-FH docked state Deposited 2025-10-13 | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
1–1016（1016 aa）
Chain B
1–1016（1016 aa）
|
Not recorded | 2OW methyl 4-(2-fluoro-3-{[(6-methylpyridin-3-yl)carbamoyl]amino}benzyl)piperazine-1-carboxylate × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 9YP9 Cryo-EM structure of human beta-cardiac myosin bound to mavacamten in the interacting-heads motif and S2-FH docked state Deposited 2025-10-13 | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
1–1016（1016 aa）
Chain B
1–1016（1016 aa）
|
Not recorded | PO4 PHOSPHATE ION × 2 XB2 Mavacamten × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9YR7 Cryo-EM structure of human beta-cardiac myosin bound to mavacamten in the interacting-heads motif and S2-FH undocked state Deposited 2025-10-16 | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
1–1016（1016 aa）
Chain B
1–1016（1016 aa）
|
Not recorded | XB2 Mavacamten × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9YRG Cryo-EM structure of human beta-cardiac myosin in the interacting-heads motif and S2-FH undocked state Deposited 2025-10-16 | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
1–1016（1016 aa）
Chain B
1–1016（1016 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9YRH Cryo-EM structure of human beta-cardiac myosin bound to omecamtiv mecarbil in the interacting-heads motif and S2-FH undocked state Deposited 2025-10-16 | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
1–1016（1016 aa）
Chain B
1–1016（1016 aa）
|
Not recorded | 2OW methyl 4-(2-fluoro-3-{[(6-methylpyridin-3-yl)carbamoyl]amino}benzyl)piperazine-1-carboxylate × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |