Current Protein Identity:Q71DI3 New Search
Main Difference Dimensions in This Set
Different construct Different mutation/modification Different assembly state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
10XZ Non-damaged nucleosome Deposited 2026-02-12 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
10YA Nucleosome containing a nick at SHL-4 Deposited 2026-02-12 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: 11-meric(11) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
10YB Nucleosome containing a nick at SHL-2 Deposited 2026-02-12 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: 11-meric(11) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
10YC Nucleosome containing a nick at SHL-4 Deposited 2026-02-12 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: 11-meric(11) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
10YD Nucleosome containing a nick at SHL-6 Deposited 2026-02-12 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: 11-meric(11) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
10YE DNA Ligase IIIa bound to nucleosome containing a nick at SHL0 Deposited 2026-02-12 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: 12-meric(12) Consistent with all polymers
Chain A 39–135(97 aa)
Chain E 39–135(97 aa)
Not recorded AMP ADENOSINE MONOPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
10YF DNA Ligase IIIa bound to nucleosome containing a nick at SHL-2 (composite) Deposited 2026-02-12 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: 12-meric(12) Consistent with all polymers
Chain A 39–134(96 aa)
Chain E 39–134(96 aa)
Not recorded AMP ADENOSINE MONOPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution not provided
10YG DNA Ligase IIIa bound to a nucleosome containing a nick at SHL-4 (composite) Deposited 2026-02-12 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: 12-meric(12) Consistent with all polymers
Chain A 39–135(97 aa)
Chain E 39–135(97 aa)
Not recorded AMP ADENOSINE MONOPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution not provided
10YH DNA Ligase IIIa bound to a nucleosome containing a nick at SHL-6 (composite) Deposited 2026-02-12 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: 12-meric(12) Consistent with all polymers
Chain A 39–135(97 aa)
Chain E 39–135(97 aa)
Not recorded AMP ADENOSINE MONOPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
10YI XRCC1-DNA Ligase IIIa complex bound to a nucleosome containing a nick at SHL-6 (composite) Deposited 2026-02-12 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: 12-meric(12) Consistent with all polymers
Chain A 39–135(97 aa)
Chain E 39–135(97 aa)
Not recorded AMP ADENOSINE MONOPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
2X4W Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1. Deposited 2010-02-02 Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain B 23–43(21 aa) Fragment:RESIDUES 23-43
Non-standard monomer:Yes (specific site not provided by mmCIF) FMT FORMIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5;4M SODIUM FORMATE, 0.1M TRIS (PH 8.5), 0.01M NACL
Resolution 1.50 Å R-free 0.216
2X4X Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1. Deposited 2010-02-02 Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain F 23–43(21 aa) Fragment:RESIDUES 23-43
Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5;20% PEG3350, 0.2M LITHIUM SULPHATE, 0.1M TRIS (PH 8.5) 0.01M NACL
Resolution 1.85 Å R-free 0.239
2X4X Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1. Deposited 2010-02-02 Assembly 2 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain D 23–43(21 aa) Fragment:RESIDUES 23-43
Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5;20% PEG3350, 0.2M LITHIUM SULPHATE, 0.1M TRIS (PH 8.5) 0.01M NACL
Resolution 1.85 Å R-free 0.239
2X4X Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1. Deposited 2010-02-02 Assembly 3 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain H 23–43(21 aa) Fragment:RESIDUES 23-43
Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5;20% PEG3350, 0.2M LITHIUM SULPHATE, 0.1M TRIS (PH 8.5) 0.01M NACL
Resolution 1.85 Å R-free 0.239
2X4X Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1. Deposited 2010-02-02 Assembly 4 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain B 23–43(21 aa) Fragment:RESIDUES 23-43
Non-standard monomer:Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5;20% PEG3350, 0.2M LITHIUM SULPHATE, 0.1M TRIS (PH 8.5) 0.01M NACL
Resolution 1.85 Å R-free 0.239
2X4Y Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1. Deposited 2010-02-02 Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain P 23–43(21 aa) Fragment:RESIDUES 23-43
Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5;30% PEG4K, 0.2M LITHIUM SULPHATE, 0.1M TRIS (PH 8.5), 0.01M NACL.
Resolution 1.70 Å R-free 0.227
2X4Y Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1. Deposited 2010-02-02 Assembly 2 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain F 23–43(21 aa) Fragment:RESIDUES 23-43
Non-standard monomer:Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5;30% PEG4K, 0.2M LITHIUM SULPHATE, 0.1M TRIS (PH 8.5), 0.01M NACL.
Resolution 1.70 Å R-free 0.227
2X4Y Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1. Deposited 2010-02-02 Assembly 3 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain D 23–43(21 aa) Fragment:RESIDUES 23-43
Non-standard monomer:Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5;30% PEG4K, 0.2M LITHIUM SULPHATE, 0.1M TRIS (PH 8.5), 0.01M NACL.
Resolution 1.70 Å R-free 0.227
2X4Y Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1. Deposited 2010-02-02 Assembly 4 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain H 23–43(21 aa) Fragment:RESIDUES 23-43
Non-standard monomer:Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5;30% PEG4K, 0.2M LITHIUM SULPHATE, 0.1M TRIS (PH 8.5), 0.01M NACL.
Resolution 1.70 Å R-free 0.227
2X4Y Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1. Deposited 2010-02-02 Assembly 5 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain L 23–43(21 aa) Fragment:RESIDUES 23-43
Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5;30% PEG4K, 0.2M LITHIUM SULPHATE, 0.1M TRIS (PH 8.5), 0.01M NACL.
Resolution 1.70 Å R-free 0.227
2X4Y Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1. Deposited 2010-02-02 Assembly 6 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain N 23–43(21 aa) Fragment:RESIDUES 23-43
Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5;30% PEG4K, 0.2M LITHIUM SULPHATE, 0.1M TRIS (PH 8.5), 0.01M NACL.
Resolution 1.70 Å R-free 0.227
2X4Y Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1. Deposited 2010-02-02 Assembly 7 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain J 23–43(21 aa) Fragment:RESIDUES 23-43
Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5;30% PEG4K, 0.2M LITHIUM SULPHATE, 0.1M TRIS (PH 8.5), 0.01M NACL.
Resolution 1.70 Å R-free 0.227
2X4Y Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1. Deposited 2010-02-02 Assembly 8 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain B 23–43(21 aa) Fragment:RESIDUES 23-43
Non-standard monomer:Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5;30% PEG4K, 0.2M LITHIUM SULPHATE, 0.1M TRIS (PH 8.5), 0.01M NACL.
Resolution 1.70 Å R-free 0.227
3AV1 The human nucleosome structure containing the histone variant H3.2 Deposited 2011-02-18 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP;pH 6;293 K;potassium cacodylate, potassium chloride, manganese chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å R-free 0.290
3DB3 Crystal structure of the tandem tudor domains of the E3 ubiquitin-protein ligase UHRF1 in complex with trimethylated histone H3-K9 peptide Deposited 2008-05-30 Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain B 7–12(6 aa)
Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP;pH 5;298 K;3 M SODIUM FORMATE, 0.1 M SODIUM ACETATE, pH 5.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.40 Å R-free 0.282
3MO8 PWWP Domain of Human Bromodomain and PHD finger-containing protein 1 In Complex with Trimethylated H3K36 Peptide Deposited 2010-04-22 Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain B 31–42(12 aa) Fragment:UNP residue 31-42
Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP;pH 8.5;293 K;3.5M sodium formate, 0.1M Tris-HCl pH8.5, H3K36me3 peptide, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.69 Å R-free 0.207
4MZF Crystal structure of human Spindlin1 bound to histone H3(K4me3-R8me2a) peptide Deposited 2013-09-30 Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain A 2–10(9 aa)
Non-standard monomer:Yes (specific site not provided by mmCIF) CL CHLORIDE ION × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP;pH 8.5;291.15 K;20% PEG 8000, 20% PEG 400, 0.1M MgCl2, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K
Resolution 2.10 Å R-free 0.255
4MZG Crystal structure of human Spindlin1 bound to histone H3K4me3 peptide Deposited 2013-09-30 Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain A 2–21(20 aa)
Non-standard monomer:Yes (specific site not provided by mmCIF) MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 MG MAGNESIUM ION × 1 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP;pH 8.5;291.15 K;37.5% MPD_P1K_P3350, 0.1M bicine/Trizma, pH 8.5, 0.06M MgCl2/CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K
Resolution 1.70 Å R-free 0.207
4MZG Crystal structure of human Spindlin1 bound to histone H3K4me3 peptide Deposited 2013-09-30 Assembly 2 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain C 2–21(20 aa)
Non-standard monomer:Yes (specific site not provided by mmCIF) MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP;pH 8.5;291.15 K;37.5% MPD_P1K_P3350, 0.1M bicine/Trizma, pH 8.5, 0.06M MgCl2/CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K
Resolution 1.70 Å R-free 0.207
4MZH Crystal structure of human Spindlin1 bound to histone H3(K4me3-R8me2s) peptide Deposited 2013-09-30 Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain B 2–10(9 aa)
Non-standard monomer:Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP;pH 8.5;291.15 K;20% PEG 8000, 20% PEG 400, 0.1M MgCl2, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K
Resolution 2.20 Å R-free 0.247
4OUC Structure of human haspin in complex with histone H3 substrate Deposited 2014-02-15 Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain B 2–13(12 aa) Fragment:histone H3 tail, UNP residues 2-13
Not recorded NA SODIUM ION × 1 EDO 1,2-ETHANEDIOL × 6 5ID (2R,3R,4S,5R)-2-(4-AMINO-5-IODO-7H-PYRROLO[2,3-D]PYRIMIDIN-7-YL)-5-(HYDROXYMETHYL)TETRAHYDROFURAN-3,4-DIOL × 1 IOD IODIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP;pH 7.5;277.15 K;20% PEG 3350, 0.2M potassium thiocyanate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K
Resolution 1.90 Å R-free 0.211
5B0Y Crystal structure of the nucleosome containing histone H3 with the crotonylated lysine 122 Deposited 2015-11-13 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) CL CHLORIDE ION × 2 MN MANGANESE (II) ION × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP;pH 6;293 K;potassium cacodylate, potassium chloride, manganese chloride
Resolution 2.56 Å R-free 0.249
5B0Z The crystal structure of the nucleosome containing H3.2, at 1.98 A resolution Deposited 2015-11-14 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded CL CHLORIDE ION × 4 MN MANGANESE (II) ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP;pH 6;293 K;potassium cacodylate, potassium chloride, manganese chloride
Resolution 1.99 Å R-free 0.254
5B40 The nucleosome structure containing H2B-K120 and H4-K31 monoubiquitinations Deposited 2016-03-22 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Mutation:C110A Mutation:C110A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP;293 K;90mM Tris-HCl (pH 7.8), 3.6% PGA-LM, 25.2% PEG 400, 2-6% Pentaerythritol ethoxylate (3/4 EO/OH)
Resolution 3.33 Å R-free 0.263
5BO0 Crystal structure of Human MCM2 HBD and ASF1b chaperoning a histone H3.2-H4 dimer Deposited 2015-05-26 Assembly 1 Protein heterocomplex Heteromer;Protein × 4 PDB declaration: tetrameric(4) Consistent with protein count
Chain A 57–136(80 aa) Fragment:UNP residues 57-136
Not recorded GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP;pH 8.5;293 K;3.2 M sodium formate, 0.1 M Tris, pH 8.5
Resolution 2.91 Å R-free 0.238
5VAC Crystal Structure of ATXR5 SET domain in complex with K36me3 histone H3 peptide Deposited 2017-03-24 Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain C 19–37(19 aa) Fragment:residues 19-37
Not recorded SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 DMS DIMETHYL SULFOXIDE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP;298 K;50% polypropylene glycol 400, 5% DMSO, 0.1 M HEPES-NaOH (pH 6.0)
Resolution 1.95 Å R-free 0.228
6FML CryoEM Structure INO80core Nucleosome complex Deposited 2018-01-31 Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: eicosameric(20) Consistent with all polymers
Chain M 2–136(135 aa)
Chain Q 2–136(135 aa)
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8;20 mM HEPES pH 8, 60 mM KCl, 0.5% glycerol, 0.25 mM CaCl2, 20 uM ZnCl2, 0.25 mM DTT, 0.05% Octyl-beta-glucoside
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.34 Å
6T79 Structure of a human nucleosome at 3.2 A resolution Deposited 2019-10-21 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE;The sample was applied onto glow-discharged Quantifoil holey carbon grids. The grids were blotted from both sides for 5-10 seconds at 16*C in a chamber at 100% humidity and plunge-frozen into liquid ethane using a manual plunger.
Resolution 3.20 Å
6T7A Structure of human Sox11 transcription factor in complex with a nucleosome Deposited 2019-10-21 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE;The sample was applied onto glow-discharged Quantifoil holey carbon grids. The grids were blotted from both sides for 5-10 seconds at 16*C in a chamber at 100% humidity and plunge-frozen into liquid ethane using a manual plunger.
Resolution 3.70 Å
6T7B Structure of human Sox2 transcription factor in complex with a nucleosome Deposited 2019-10-21 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE;The sample was applied onto glow-discharged Quantifoil holey carbon grids. The grids were blotted from both sides for 5-10 seconds at 16*C in a chamber at 100% humidity and plunge-frozen into liquid ethane using a manual plunger.
Resolution 5.10 Å
6T7C Structure of two copies of human Sox11 transcription factor in complex with a nucleosome Deposited 2019-10-21 Assembly 1 Protein–DNA Heteromer;Protein × 10 PDB declaration: dodecameric(12) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE;The sample was applied onto glow-discharged Quantifoil holey carbon grids. The grids were blotted from both sides for 5-10 seconds at 16*C in a chamber at 100% humidity and plunge-frozen into liquid ethane using a manual plunger.
Resolution 4.00 Å
6T7D Structure of human Sox11 transcription factor in complex with a nucleosome Deposited 2019-10-21 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE;The sample was applied onto glow-discharged Quantifoil holey carbon grids. The grids were blotted from both sides for 5-10 seconds at 16*C in a chamber at 100% humidity and plunge-frozen into liquid ethane using a manual plunger.
Resolution 4.40 Å
6X59 The mouse cGAS catalytic domain binding to human assembled nucleosome Deposited 2020-05-25 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.98 Å
6X5A The mouse cGAS catalytic domain binding to human nucleosome that purified from HEK293T cells Deposited 2020-05-25 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.36 Å
6XJD Two mouse cGAS catalytic domain binding to human assembled nucleosome Deposited 2020-06-23 Assembly 1 Protein–DNA Heteromer;Protein × 10 PDB declaration: dodecameric(12) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6Y5D Structure of human cGAS (K394E) bound to the nucleosome Deposited 2020-02-25 Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: 22-meric(22) Consistent with all polymers
Chain A 39–136(98 aa)
Chain E 39–136(98 aa)
Chain M 39–136(98 aa)
Chain Q 39–136(98 aa)
Not recorded PTD PENTANEDIAL × 9 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
6Y5E Structure of human cGAS (K394E) bound to the nucleosome (focused refinement of cGAS-NCP subcomplex) Deposited 2020-02-25 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain A 39–134(96 aa)
Chain E 39–134(96 aa)
Not recorded PTD PENTANEDIAL × 8 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.15 Å
7BQZ Crystal Structure of Spindlin1 bound to H3(K4me3-K9me3) peptide Deposited 2020-03-26 Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain B 2–16(15 aa)
Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION;pH 7;291 K;2.8M sodium acetate, pH7.0
Resolution 3.10 Å R-free 0.264
7BQZ Crystal Structure of Spindlin1 bound to H3(K4me3-K9me3) peptide Deposited 2020-03-26 Assembly 2 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain D 2–16(15 aa)
Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION;pH 7;291 K;2.8M sodium acetate, pH7.0
Resolution 3.10 Å R-free 0.264
7BQZ Crystal Structure of Spindlin1 bound to H3(K4me3-K9me3) peptide Deposited 2020-03-26 Assembly 3 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain F 2–16(15 aa)
Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION;pH 7;291 K;2.8M sodium acetate, pH7.0
Resolution 3.10 Å R-free 0.264
7BQZ Crystal Structure of Spindlin1 bound to H3(K4me3-K9me3) peptide Deposited 2020-03-26 Assembly 4 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain H 2–16(15 aa)
Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION;pH 7;291 K;2.8M sodium acetate, pH7.0
Resolution 3.10 Å R-free 0.264
7BU9 Crystal Structure of Spindlin1-H3(K4me3-K9me2) complex Deposited 2020-04-05 Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain B 2–16(15 aa)
Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION;pH 7;291 K;3.6M sodium formate, 3% DMSO
Resolution 3.50 Å R-free 0.264
7BU9 Crystal Structure of Spindlin1-H3(K4me3-K9me2) complex Deposited 2020-04-05 Assembly 2 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain D 2–16(15 aa)
Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION;pH 7;291 K;3.6M sodium formate, 3% DMSO
Resolution 3.50 Å R-free 0.264
7BU9 Crystal Structure of Spindlin1-H3(K4me3-K9me2) complex Deposited 2020-04-05 Assembly 3 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain F 2–16(15 aa)
Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION;pH 7;291 K;3.6M sodium formate, 3% DMSO
Resolution 3.50 Å R-free 0.264
7BU9 Crystal Structure of Spindlin1-H3(K4me3-K9me2) complex Deposited 2020-04-05 Assembly 4 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain H 2–16(15 aa)
Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION;pH 7;291 K;3.6M sodium formate, 3% DMSO
Resolution 3.50 Å R-free 0.264
7JO9 1:1 cGAS-nucleosome complex Deposited 2020-08-06 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7JOA 2:1 cGAS-nucleosome complex Deposited 2020-08-06 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7JZV Cryo-EM structure of the BRCA1-UbcH5c/BARD1 E3-E2 module bound to a nucleosome Deposited 2020-09-02 Assembly 1 Insufficient information Heteromer;Protein × 10 PDB declaration: dodecameric(12) Consistent with all polymers
Chain P 2–136(135 aa)
Chain p 2–136(135 aa)
Mutation:C110A Mutation:C110A ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
7PET The 4x177 nucleosome array containing H1 Deposited 2021-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 34 PDB declaration: 36-meric(36) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Chain K 1–136(136 aa)
Chain O 1–136(136 aa)
Chain a 1–136(136 aa)
Chain e 1–136(136 aa)
Chain k 1–136(136 aa)
Chain o 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.50 Å
7PEU Trinucleosome of the 4x177 nucleosome array containing H1 Deposited 2021-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 25 PDB declaration: 27-meric(27) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Chain K 1–136(136 aa)
Chain O 1–136(136 aa)
Chain a 1–136(136 aa)
Chain e 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.20 Å
7PEV Nucleosome stack of the 4x177 nucleosome array containing H1 Deposited 2021-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 16 PDB declaration: octadecameric(18) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Chain K 1–136(136 aa)
Chain O 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.00 Å
7PEW Nucleosome 1 of the 4x177 nucleosome array containing H1 Deposited 2021-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7PEX Nucleosome 2 of the 4x177 nucleosome array containing H1 Deposited 2021-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain a 1–136(136 aa)
Chain e 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.10 Å
7PEY Nucleosome 3 of the 4x177 nucleosome array containing H1 Deposited 2021-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain K 1–136(136 aa)
Chain O 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
7PEZ Nucleosome 4 of the 4x177 nucleosome array containing H1 Deposited 2021-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain k 1–136(136 aa)
Chain o 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.90 Å
7PF0 Trinucleosome of the 4x177 nucleosome array containing H1 Deposited 2021-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 26 PDB declaration: 28-meric(28) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Chain K 1–136(136 aa)
Chain O 1–136(136 aa)
Chain a 1–136(136 aa)
Chain e 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 11.00 Å
7PF2 Nucleosome stack of the 4x187 nucleosome array containing H1 Deposited 2021-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 17 PDB declaration: nonadecameric(19) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Chain K 1–136(136 aa)
Chain O 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.10 Å
7PF3 Nucleosome 4 of the 4x187 nucleosome array containing H1 Deposited 2021-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain k 1–136(136 aa)
Chain o 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
7PF4 Nucleosome 3 of the 4x187 nucleosome array containing H1 Deposited 2021-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain K 1–136(136 aa)
Chain O 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
7PF5 Nucleosome 2 of the 4x187 nucleosome array containing H1 Deposited 2021-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain a 1–136(136 aa)
Chain e 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7PF6 Nucleosome 1 of the 4x187 nucleosome array containing H1 Deposited 2021-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
7PFA Trinucleosome of the 4x197 nucleosome array containing H1 Deposited 2021-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 26 PDB declaration: 28-meric(28) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Chain K 1–136(136 aa)
Chain O 1–136(136 aa)
Chain a 1–136(136 aa)
Chain e 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.70 Å
7PFC Nucleosome stack of the 4x197 nucleosome array containing H1 Deposited 2021-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 17 PDB declaration: nonadecameric(19) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Chain K 1–136(136 aa)
Chain O 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.40 Å
7PFD Nucleosome 1 of the 4x197 nucleosome array containing H1 Deposited 2021-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
7PFE Nucleosome 2 of the 4x197 nucleosome array containing H1 Deposited 2021-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain a 1–136(136 aa)
Chain e 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
7PFF Nucleosome 3 of the 4x197 nucleosome array containing H1 Deposited 2021-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain K 1–136(136 aa)
Chain O 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
7PFT Trinucleosome of the 4x207 nucleosome array containing H1 Deposited 2021-08-12 Assembly 1 Protein–DNA Heteromer;Protein × 27 PDB declaration: 29-meric(29) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Chain K 1–136(136 aa)
Chain O 1–136(136 aa)
Chain a 1–136(136 aa)
Chain e 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.80 Å
7PFU Nucleosome stack of the 4x207 nucleosome array containing H1 Deposited 2021-08-12 Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: eicosameric(20) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Chain K 1–136(136 aa)
Chain O 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.00 Å
7PFV Nucleosome 1 of the 4x207 nucleosome array containing H1 Deposited 2021-08-12 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
7PFW Nucleosome 2 of the 4x207 nucleosome array containing H1 Deposited 2021-08-12 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain a 1–136(136 aa)
Chain e 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.20 Å
7PFX Nucleosome 3 of the 4x207 nucleosome array containing H1 Deposited 2021-08-12 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain K 1–136(136 aa)
Chain O 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
7TAN Structure of VRK1 C-terminal tail bound to nucleosome core particle Deposited 2021-12-21 Assembly 1 Protein–DNA Heteromer;Protein × 10 PDB declaration: dodecameric(12) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
7U50 APE1 bound to a nucleosome core particle with AP-site at SHL-6 Deposited 2022-03-01 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
7U51 Nucleosome core particle with AP-site at SHL-6 Deposited 2022-03-01 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7U52 nucleosome core particle with AP-site at SHL-6.5 Deposited 2022-03-01 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
7U53 Nucleosome core particle with AP-site at SHL0 Deposited 2022-03-01 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
7UV9 KDM2A-nucleosome structure stabilized by H3K36C-UNC8015 covalent conjugate Deposited 2022-04-29 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Mutation:K36C Mutation:K36C OH0 N-heptanoyl-N-hydroxy-beta-alanine × 1 FE FE (III) ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.20 Å
7UVA Crystal structure of KDM2A histone demethylase catalytic domain in complex with an H3C36 peptide modified by UNC8015 Deposited 2022-04-29 Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count
Chain C 30–42(13 aa) Fragment:UNP residues 30-42
Mutation:K36C FE FE (III) ION × 1 OH0 N-heptanoyl-N-hydroxy-beta-alanine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP;pH 8.5;293 K;100 mM Tris-Cl, pH 8.5, 150 mM lithium sulfate, 20% PEG3350
Resolution 1.98 Å R-free 0.237
7UVA Crystal structure of KDM2A histone demethylase catalytic domain in complex with an H3C36 peptide modified by UNC8015 Deposited 2022-04-29 Assembly 2 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count
Chain F 30–42(13 aa) Fragment:UNP residues 30-42
Mutation:K36C FE FE (III) ION × 1 OH0 N-heptanoyl-N-hydroxy-beta-alanine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP;pH 8.5;293 K;100 mM Tris-Cl, pH 8.5, 150 mM lithium sulfate, 20% PEG3350
Resolution 1.98 Å R-free 0.237
8ATF Nucleosome-bound Ino80 ATPase Deposited 2022-08-23 Assembly 1 Protein–DNA Heteromer;Protein × 10 PDB declaration: dodecameric(12) Consistent with all polymers
Chain M 2–136(135 aa)
Chain Q 2–136(135 aa)
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.45 Å
8AV6 CryoEM structure of INO80 core nucleosome complex in closed grappler conformation Deposited 2022-08-26 Assembly 1 Protein–DNA Heteromer;Protein × 18 PDB declaration: eicosameric(20) Consistent with all polymers
Chain M 2–136(135 aa)
Chain Q 2–136(135 aa)
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.68 Å
8JLB Cryo-EM structure of the 145 bp human nucleosome containing H3.2 C110A mutant Deposited 2023-06-02 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Mutation:C110A Mutation:C110A No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.36 Å
8JLD Cryo-EM structure of the 145 bp human nucleosome containing acetylated H3 tail Deposited 2023-06-02 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Mutation:C110A Non-standard monomer:Yes (specific site not provided by mmCIF) Mutation:C110A Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.48 Å
8OL1 cGAS-Nucleosome in complex with SPSB3-ELOBC (composite structure) Deposited 2023-03-29 Assembly 1 Protein–DNA Heteromer;Protein × 12 PDB declaration: tetradecameric(14) Consistent with all polymers
Chain A 38–135(98 aa)
Chain E 38–135(98 aa)
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4;PBS buffer
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8VMJ H3K4me3 nucleosome bound to PRC2_AJ119-450 Deposited 2024-01-13 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain I 1–136(136 aa)
Chain O 1–136(136 aa)
Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VMN H3K4me3 nucleosome bound to PRC2_AJ1-450 Deposited 2024-01-13 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain I 1–136(136 aa)
Chain O 1–136(136 aa)
Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8VO0 H3K36me3-modified nucleosome bound to PRC2_AJ1-450 with histone H3 tail disengaged Deposited 2024-01-14 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain I 42–136(95 aa)
Chain O 42–136(95 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VOB H3K36me3-modified nucleosome bound to PRC2_AJ1-450 Deposited 2024-01-14 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain I 1–136(136 aa)
Chain O 1–136(136 aa)
Non-standard monomer:Yes (specific site not provided by mmCIF) Non-standard monomer:Yes (specific site not provided by mmCIF) No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VWS Nucleosome containing 8oxoG at SHL-6 Deposited 2024-02-02 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.1;50 mM HEPES (pH-7.1), 100 mM NaCl, 1 mM TCEP, and 1 mM EDTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VWT OGG1 bound to a nucleosome containing 8oxoG at SHL-6 (composite map) Deposited 2024-02-02 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: 11-meric(11) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.1;50 mM HEPES (pH-7.1), 100 mM NaCl, 1 mM TCEP, and 1 mM EDTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VWU Nucleosome containing 8oxoG at SHL4 Deposited 2024-02-02 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.1;50 mM HEPES (pH-7.1), 100 mM NaCl, 1 mM TCEP, and 1 mM EDTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8VWV OGG1 bound to a nucleosome containing 8oxoG at SHL4 (composite map) Deposited 2024-02-02 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: 11-meric(11) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.1;50 mM HEPES (pH-7.1), 100 mM NaCl, 1 mM TCEP, and 1 mM EDTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8X7I Cryo-EM structures of RNF168/UbcH5c-Ub in complex with H2AK13Ub nucleosomes determined by intein-based E2-Ub-NCP conjugation strategy Deposited 2023-11-24 Assembly 1 Protein–DNA Heteromer;Protein × 11 PDB declaration: tridecameric(13) Consistent with all polymers
Chain A 38–135(98 aa)
Chain E 38–135(98 aa)
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.27 Å
8X7J Cryo-EM structures of RNF168/UbcH5c-Ub/nucleosomes complex determined by activity-based chemical trapping strategy Deposited 2023-11-24 Assembly 1 Protein–DNA Heteromer;Protein × 11 PDB declaration: tridecameric(13) Consistent with all polymers
Chain A 39–135(97 aa)
Chain E 39–135(97 aa)
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.39 Å
8X7K Cryo-EM structures of RNF168/UbcH5c-Ub in complex with H2AK13Ub nucleosomes determined by activity-based chemical trapping strategy (adjacent H2AK13/15 dual-monoubiquitination) Deposited 2023-11-24 Assembly 1 Protein–DNA Heteromer;Protein × 10 PDB declaration: dodecameric(12) Consistent with all polymers
Chain A 39–135(97 aa)
Chain E 39–135(97 aa)
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.27 Å
8ZJR Structure of nucleosome-bound RFX5 complex Deposited 2024-05-15 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8ZJT Structure of free nucleosome Deposited 2024-05-15 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9BE5 Cryo-EM structure of Human Nucleosome collected by EPU on Glacios at 3.3 Angstrom resolution Deposited 2024-04-14 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 39–135(97 aa)
Chain E 39–136(98 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9BE6 Cryo-EM structure of Human Nucleosome collected by Leginon on Krios at 3.0 Angstrom resolution Deposited 2024-04-14 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 39–135(97 aa)
Chain E 40–136(97 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9D3K Two Dsup molecules in complex with the nucleosome open from both sides Deposited 2024-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 10 PDB declaration: dodecameric(12) Consistent with all polymers
Chain A 42–136(95 aa)
Chain E 42–136(95 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
9D3L Two Dsup molecules in complex with the nucleosome open from the left side Deposited 2024-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 10 PDB declaration: dodecameric(12) Consistent with all polymers
Chain A 39–136(98 aa)
Chain E 39–136(98 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9D3M Two HMGN2s in complex with the nucleosome Deposited 2024-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 10 PDB declaration: dodecameric(12) Consistent with all polymers
Chain A 38–136(99 aa)
Chain E 38–136(99 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9D3N 167-bp 5S rDNA nucleosome cross-linked with glutaraldehyde Deposited 2024-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 45–134(90 aa)
Chain E 45–134(90 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9D3O 167-bp 5S rDNA nucleosome - closed Deposited 2024-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 38–136(99 aa)
Chain E 38–136(99 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9D3P 167-bp 5S rDNA nucleosome - open I (open only on the downstream side) Deposited 2024-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 38–136(99 aa)
Chain E 38–136(99 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
9D3Q 167-bp 5S rDNA nucleosome - open II Deposited 2024-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 41–136(96 aa)
Chain E 41–136(96 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9D3R 147-bp 5S rDNA nucleosome - closed Deposited 2024-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 40–136(97 aa)
Chain E 40–136(97 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9D3S 147-bp 5S rDNA nucleosome - open I (open on the downstream side) Deposited 2024-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 38–136(99 aa)
Chain E 38–136(99 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9D3T 147-bp 5S rDNA nucleosome cross-linked with glutaraldehyde Deposited 2024-08-11 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 44–136(93 aa)
Chain E 44–136(93 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9DWF Nucleosome containing a 1-nt gap at SHL-4.5 Deposited 2024-10-09 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: 11-meric(11) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.1;25mM HEPES (pH-7.1), 50 mM NaCl, 1mM TCEP, and 5mM EDTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9DWG DNA Polymerase Beta bound to a nucleosome containing a 1-nt gap at SHL-4.5 (State 1, composite) Deposited 2024-10-09 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: 12-meric(12) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.1;25mM HEPES (pH-7.1), 50 mM NaCl, 1mM TCEP, and 5mM EDTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9DWH DNA Polymerase Beta bound to a nucleosome containing a 1-nt gap at SHL-4.5 (State 2, composite) Deposited 2024-10-09 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: 12-meric(12) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.1;25mM HEPES (pH-7.1), 50 mM NaCl, 1mM TCEP, and 5mM EDTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9DWI DNA Polymerase Beta bound to a nucleosome containing a 1-nt gap at SHL-4.5 (State 3, composite) Deposited 2024-10-09 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: 12-meric(12) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.1;25mM HEPES (pH-7.1), 50 mM NaCl, 1mM TCEP, and 5mM EDTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9DWJ Nucleosome containing a 1-nt gap at SHL-3.5 Deposited 2024-10-09 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: undecameric(11) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.1;25mM HEPES (pH-7.1), 50 mM NaCl, 1mM TCEP, and 5mM EDTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
9DWK DNA Polymerase Beta bound to a nucleosome containing a 1-nt gap at SHL-3.5 Deposited 2024-10-09 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: dodecameric(12) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.1;25mM HEPES (pH-7.1), 50 mM NaCl, 1mM TCEP, and 5mM EDTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
9DWL Nucleosome containing a 1-nt gap at SHL-5.5 Deposited 2024-10-09 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: undecameric(11) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.1;25mM HEPES (pH-7.1), 50mM NaCl, 1mM TCEP, and 5mM EDTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
9DWM DNA polymerase Beta bound to a nucleosome containing a 1-nt gap at SHL-5.5 Deposited 2024-10-09 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: dodecameric(12) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.1;25mM HEPES (pH-7.1), 50mM NaCl, 1mM TCEP, and 5mM EDTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
9E00 Cryo-EM structure of human DNMT3A2-DNMT3B3 complex bound to di-nucleosome Deposited 2024-10-17 Assembly 1 Protein–DNA Heteromer;Protein × 24 PDB declaration: 26-meric(26) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Chain M 2–136(135 aa)
Chain Q 2–136(135 aa)
Not recorded ZN ZINC ION × 9 SAH S-ADENOSYL-L-HOMOCYSTEINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.75 Å
9E05 The consensus model of the cryo-EM structure of human DNMT3A2-DNMT3B3 complex bound to di-nucleosome Deposited 2024-10-17 Assembly 1 Protein–DNA Heteromer;Protein × 24 PDB declaration: 26-meric(26) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Chain M 2–136(135 aa)
Chain Q 2–136(135 aa)
Not recorded ZN ZINC ION × 18 SAH S-ADENOSYL-L-HOMOCYSTEINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.00 Å
9E09 Cryo-EM structure a single nucleosome (2) focus of human DNMT3A2-DNMT3B3 complex bound to di-nucleosome Deposited 2024-10-17 Assembly 1 Protein–DNA Heteromer;Protein × 10 PDB declaration: 12-meric(12) Consistent with all polymers
Chain M 2–136(135 aa)
Chain Q 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.72 Å
9E0R Cryo-EM structure of a single nucleosome (1) focus of human DNMT3A2-DNMT3B3 complex bound to di-nucleosome Deposited 2024-10-18 Assembly 1 Protein–DNA Heteromer;Protein × 10 PDB declaration: 12-meric(12) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9E2D Cryo-EM structure of human DNMT3A2-DNMT3B3 complex bound to a di-nucleosome with a five base pair linker Deposited 2024-10-22 Assembly 1 Protein–DNA Heteromer;Protein × 24 PDB declaration: 26-meric(26) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Chain M 2–136(135 aa)
Chain Q 2–136(135 aa)
Not recorded ZN ZINC ION × 18 SAH S-ADENOSYL-L-HOMOCYSTEINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.08 Å
9E2F Cryo-EM structure of a di-nucleosome with a five base pair linker Deposited 2024-10-22 Assembly 1 Protein–DNA Heteromer;Protein × 16 PDB declaration: 18-meric(18) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Chain M 2–136(135 aa)
Chain Q 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å
9E2Q Cryo-EM structure of DNMT 3A2/3B3 tetramer in complex with a di-nucleosome with a six base pair linker Deposited 2024-10-22 Assembly 1 Protein–DNA Heteromer;Protein × 24 PDB declaration: 26-meric(26) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Chain M 2–136(135 aa)
Chain Q 2–136(135 aa)
Not recorded ZN ZINC ION × 18 SAH S-ADENOSYL-L-HOMOCYSTEINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.14 Å
9E2R Cryo-EM structure of human DNMT3A2-DNMT3B3 complex bound to a di-nucleosome and an histone-3 peptide Deposited 2024-10-22 Assembly 1 Protein–DNA Heteromer;Protein × 24 PDB declaration: 26-meric(26) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Chain M 2–136(135 aa)
Chain Q 2–136(135 aa)
Not recorded ZN ZINC ION × 18 SAH S-ADENOSYL-L-HOMOCYSTEINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.71 Å
9E3D Cryo-EM structure of DNMT 3A2/3B3 tetramer in complex with a di-nucleosome with K120R mutant H2B Deposited 2024-10-23 Assembly 1 Protein–DNA Heteromer;Protein × 20 PDB declaration: 22-meric(22) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Chain M 2–136(135 aa)
Chain Q 2–136(135 aa)
Not recorded ZN ZINC ION × 9 SAH S-ADENOSYL-L-HOMOCYSTEINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
9E3R Cryo-EM structure of PWWP domain deleted DNMT 3A2/3B3 in complex with a di-nucleosome Deposited 2024-10-23 Assembly 1 Protein–DNA Heteromer;Protein × 24 PDB declaration: 26-meric(26) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Chain M 2–136(135 aa)
Chain Q 2–136(135 aa)
Not recorded ZN ZINC ION × 18 SAH S-ADENOSYL-L-HOMOCYSTEINE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.90 Å
9E3U Cryo-EM structure of DNMT 3A2/3B3 tetramer bound to a di-nucleosome with a 25 base-pair linker Deposited 2024-10-24 Assembly 1 Protein–DNA Heteromer;Protein × 13 PDB declaration: 15-meric(15) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded ZN ZINC ION × 9 SAH S-ADENOSYL-L-HOMOCYSTEINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.90 Å
9E49 Cryo-EM structure of a di-nucleosome with an eight base-pair linker Deposited 2024-10-24 Assembly 1 Protein–DNA Heteromer;Protein × 16 PDB declaration: 18-meric(18) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Chain M 2–136(135 aa)
Chain Q 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.93 Å
9GMK SIRT7:H3K18DTU nucleosome complex Deposited 2024-08-29 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: 11-meric(11) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
9GMR SIRT7-H3K36MTUnucleosome complex Deposited 2024-08-29 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded A1IY0 [[(2~{R},3~{a}~{R},5~{R},6~{R},6~{a}~{R})-2-(methylamino)-6-oxidanyl-2-(propylamino)-3~{a},5,6,6~{a}-tetrahydrofuro[2,3-d][1,3]oxathiol-5-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9GW2 State 2 MAP 1 SETD2 bound to proximal H3 of upstream nucleosome Deposited 2024-09-26 Assembly 1 Protein–DNA Heteromer;Protein × 10 PDB declaration: 12-meric(12) Consistent with all polymers
Chain a 1–136(136 aa)
Chain e 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.84 Å
9IPU cryo-EM structure of the RNF168(1-193)/UbcH5c-Ub ubiquitylation module bound to H1.0-K63-Ub3 modified chromatosome Deposited 2024-07-11 Assembly 1 Protein–DNA Heteromer;Protein × 12 PDB declaration: tetradecameric(14) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
9NQU KDM6B-nucleosome structure stabilized by H3K27C-UNC8015 covalent conjugate Deposited 2025-03-13 Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: 11-meric(11) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded FE FE (III) ION × 1 ZN ZINC ION × 1 OH0 N-heptanoyl-N-hydroxy-beta-alanine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.16 Å
9OGR Cryo-EM analysis of human H2A.Z on methylated Sat2R-P DNA (v1) Deposited 2025-05-01 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.78 Å
9OGS Cryo-EM analysis of human H2A.Z on Sat2R-P DNA (v1) Deposited 2025-05-01 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.05 Å
9OGZ Cryo-EM analysis of human H2A.Z on Sat2R-P DNA (v2) Deposited 2025-05-02 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.05 Å
9OH0 Cryo-EM analysis of human H2A.Z on methylated Sat2R-P DNA (v2) Deposited 2025-05-02 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.78 Å
9OH1 Cryo-EM analysis of human H2A.Z on 601L DNA Deposited 2025-05-02 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.09 Å
9OH2 Cryo-EM analysis of human H2A.Z on methylated 601L DNA Deposited 2025-05-02 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 1–136(136 aa)
Chain E 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.14 Å
9RZC State 3 MAP 1 SETD2 bound to distal H3 of upstream nucleosome Deposited 2025-07-15 Assembly 1 Protein–DNA Heteromer;Protein × 10 PDB declaration: 12-meric(12) Consistent with all polymers
Chain a 1–136(136 aa)
Chain e 1–136(136 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.66 Å
9RZE State 2 MAP 3 RNA Pol II activated elongation complex with SETD2 bound to proximal upstream H3 Deposited 2025-07-15 Assembly 1 Other combination Heteromer;Protein × 31 PDB declaration: 34-meric(34) Consistent with all polymers
Chain a 1–136(136 aa)
Chain e 1–136(136 aa)
Not recorded ZN ZINC ION × 9 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.53 Å
9S0U State 3 MAP 3 RNA Pol II activated elongation complex with SETD2 bound to distal upstream H3 Deposited 2025-07-17 Assembly 1 Other combination Heteromer;Protein × 32 PDB declaration: 35-meric(35) Consistent with all polymers
Chain a 1–136(136 aa)
Chain e 1–136(136 aa)
Not recorded ZN ZINC ION × 9 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.72 Å
9S3G State 1 MAP3 RNA Pol II activated elongation complex with SETD2 and upstream hexasome Deposited 2025-07-24 Assembly 1 Other combination Heteromer;Protein × 31 PDB declaration: 34-meric(34) Consistent with all polymers
Chain a 1–136(136 aa)
Chain e 1–136(136 aa)
Not recorded ZN ZINC ION × 9 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.40 Å
9U5U The 1:1 cryo-EM structure of BAP1/ASXL1-K351Ub in complex with H2AK119Ub nucleosome Deposited 2025-03-21 Assembly 1 Protein–DNA Heteromer;Protein × 12 PDB declaration: tetradecameric(14) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.12 Å
9Y46 Human nucleosome structure on Nickel-NTA lipid affinity grid (C2 refinement) Deposited 2025-09-02 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.59 Å
9Y47 Human nucleosome structure on Nickel-NTA lipid affinity grid (C1 refinement) Deposited 2025-09-02 Assembly 1 Protein–DNA Heteromer;Protein × 8 PDB declaration: decameric(10) Consistent with all polymers
Chain A 2–136(135 aa)
Chain E 2–136(135 aa)
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.74 Å
9ZEN Competition for different elements of the nucleosome acidic patch yields distinct functional outcomes. VHH 1B2 Deposited 2025-11-30 Assembly 1 Protein–DNA Heteromer;Protein × 10 PDB declaration: 12-meric(12) Consistent with all polymers
Chain A 39–135(97 aa)
Chain E 39–135(97 aa)
Mutation:C97S Mutation:C97S No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6;20 mM HEPES pH7.6, 50 mM NaCl, 0.5 mM MgCl2, 1 mM EDTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.17 Å