7n9f

Structure of the in situ yeast NPC

Method: ELECTRON MICROSCOPY D_max: 474.6 Å Quality: EXCELLENT

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Nucleoporin NUP170

OrganismNot specified

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain 0； UniProt 1–1502 Chain Y； UniProt 1–1502	Not recorded	Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NIC96 × 32 （P34077） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP120 × 16 （P35729） Nucleoporin NUP85 × 16 （P46673） Nucleoporin 145c × 16 （P49687） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP84 × 16 （P52891） Nucleoporin NUP133 × 16 （P36161） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain 0； UniProt 1–1502 Chain Y； UniProt 1–1502	Not recorded	Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain 0； UniProt 1–1502 Chain Y； UniProt 1–1502	Not recorded	Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3I5P Nup170(aa979-1502), S.cerevisiae Deposited 2009-07-06	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 980–1502（523 aa） Fragment：HELICAL DOMAIN (UNP residues 980-1502)	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.9；289 K；0.2M AMMONIUM ACETATE, 0.1M TRIS-HCL, 8% PEG 3350, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 3.20 Å R-free 0.324
3I5Q Nup170(aa1253-1502) at 2.2 A, S.cerevisiae Deposited 2009-07-06	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 1253–1502（250 aa） Fragment：C-terminal fragment (UNP residues 1253-1502)	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；289 K；0.2M LITHIUM SULFATE, 0.1M TRIS-HCL, 50 mM NACL, 22% PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 2.20 Å R-free 0.272
3I5Q Nup170(aa1253-1502) at 2.2 A, S.cerevisiae Deposited 2009-07-06	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1253–1502（250 aa） Fragment：C-terminal fragment (UNP residues 1253-1502)	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；289 K；0.2M LITHIUM SULFATE, 0.1M TRIS-HCL, 50 mM NACL, 22% PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 2.20 Å R-free 0.272
3I5Q Nup170(aa1253-1502) at 2.2 A, S.cerevisiae Deposited 2009-07-06	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 1253–1502（250 aa） Fragment：C-terminal fragment (UNP residues 1253-1502) Chain B 1253–1502（250 aa） Fragment：C-terminal fragment (UNP residues 1253-1502)	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；289 K；0.2M LITHIUM SULFATE, 0.1M TRIS-HCL, 50 mM NACL, 22% PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 2.20 Å R-free 0.272
3I5Q Nup170(aa1253-1502) at 2.2 A, S.cerevisiae Deposited 2009-07-06	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1253–1502（250 aa） Fragment：C-terminal fragment (UNP residues 1253-1502) Chain B 1253–1502（250 aa） Fragment：C-terminal fragment (UNP residues 1253-1502)	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；289 K；0.2M LITHIUM SULFATE, 0.1M TRIS-HCL, 50 mM NACL, 22% PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 2.20 Å R-free 0.272
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 240 PDB declaration: 240-meric	Chain 0 1–1502（1502 aa） Chain Y 1–1502（1502 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain 0 1–1502（1502 aa） Chain Y 1–1502（1502 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain 0 1–1502（1502 aa） Chain Y 1–1502（1502 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7WOO Cryo-EM structure of the inner ring protomer of the Saccharomyces cerevisiae nuclear pore complex Deposited 2022-01-22	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric	Chain D 1–1502（1502 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.71 Å
7WOT Cryo-EM structure of the inner ring monomer of the Saccharomyces cerevisiae nuclear pore complex Deposited 2022-01-22	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric	Chain D 1–1502（1502 aa） Chain P 1–1502（1502 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.73 Å
8TJ5 Inner spoke ring of the yeast NPC Deposited 2023-07-20	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 46 PDB declaration: 46-meric	Chain 0 1–1502（1502 aa） Chain Y 1–1502（1502 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20mM HEPES,50mM Potassium acetate,20mM NaCl,2mM MgCl2,1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.60 Å

6 other PDB entries and 11 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	NU170_YEAST
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain 0； PDBConstruct 1–1502； UniProt 1–1502 Author chain Y； PDBConstruct 1–1502； UniProt 1–1502

Nucleoporin NUP157

OrganismNot specified

UniProt P40064

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain 1； UniProt 1–1391 Chain Z； UniProt 1–1391	Not recorded	Nucleoporin NUP170 × 16 （P38181） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NIC96 × 32 （P34077） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP120 × 16 （P35729） Nucleoporin NUP85 × 16 （P46673） Nucleoporin 145c × 16 （P49687） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP84 × 16 （P52891） Nucleoporin NUP133 × 16 （P36161） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain 1； UniProt 1–1391 Chain Z； UniProt 1–1391	Not recorded	Nucleoporin NUP170 × 2 （P38181） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain 1； UniProt 1–1391 Chain Z； UniProt 1–1391	Not recorded	Nucleoporin NUP170 × 2 （P38181） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
4MHC Crystal Structure of a Nucleoporin Deposited 2013-08-29	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 70–893（824 aa） Fragment：N-terminal domain (UNP residues 70-893)	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；5% PEG 4,000, 0.1 M MES, pH 6.0, 0.05 M Magnesium chloride, 3% (v/v) 1,4-dioxane, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 2.40 Å R-free 0.231
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 240 PDB declaration: 240-meric	Chain 1 1–1391（1391 aa） Chain Z 1–1391（1391 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain 1 1–1391（1391 aa） Chain Z 1–1391（1391 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain 1 1–1391（1391 aa） Chain Z 1–1391（1391 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7WOO Cryo-EM structure of the inner ring protomer of the Saccharomyces cerevisiae nuclear pore complex Deposited 2022-01-22	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric	Chain C 1–1391（1391 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.71 Å
7WOT Cryo-EM structure of the inner ring monomer of the Saccharomyces cerevisiae nuclear pore complex Deposited 2022-01-22	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric	Chain C 1–1391（1391 aa） Chain O 1–1391（1391 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.73 Å
8TJ5 Inner spoke ring of the yeast NPC Deposited 2023-07-20	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 46 PDB declaration: 46-meric	Chain 1 1–1391（1391 aa） Chain Z 1–1391（1391 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20mM HEPES,50mM Potassium acetate,20mM NaCl,2mM MgCl2,1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.60 Å

5 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	NU157_YEAST
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain 1； PDBConstruct 1–1391； UniProt 1–1391 Author chain Z； PDBConstruct 1–1391； UniProt 1–1391

Nucleoporin NSP1

OrganismNot specified

UniProt P14907

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain A； UniProt 1–823 Chain D； UniProt 1–823 Chain G； UniProt 1–823 Chain J； UniProt 1–823 Chain y； UniProt 1–823 Chain z； UniProt 1–823	Not recorded	Nucleoporin NUP170 × 16 （P38181） Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NIC96 × 32 （P34077） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP120 × 16 （P35729） Nucleoporin NUP85 × 16 （P46673） Nucleoporin 145c × 16 （P49687） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP84 × 16 （P52891） Nucleoporin NUP133 × 16 （P36161） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain A； UniProt 1–823 Chain D； UniProt 1–823 Chain G； UniProt 1–823 Chain J； UniProt 1–823 Chain y； UniProt 1–823 Chain z； UniProt 1–823	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain A； UniProt 1–823 Chain D； UniProt 1–823 Chain G； UniProt 1–823 Chain J； UniProt 1–823 Chain y； UniProt 1–823 Chain z； UniProt 1–823	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
1O6O Importin Beta aa1-442 bound to five FxFG repeats from yeast Nsp1p. Second crystal form Deposited 2002-10-10	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain D 497–608（112 aa） Fragment：RESIDUES 497-608	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 5.9；1.2-1.28M AMMONIUM SULPHATE, 100MM AMMONIUM ACETATE, PH 5.9, 30MM DTT	Resolution 2.80 Å R-free 0.273
1O6O Importin Beta aa1-442 bound to five FxFG repeats from yeast Nsp1p. Second crystal form Deposited 2002-10-10	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain F 497–608（112 aa） Fragment：RESIDUES 497-608	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 5.9；1.2-1.28M AMMONIUM SULPHATE, 100MM AMMONIUM ACETATE, PH 5.9, 30MM DTT	Resolution 2.80 Å R-free 0.273
1O6O Importin Beta aa1-442 bound to five FxFG repeats from yeast Nsp1p. Second crystal form Deposited 2002-10-10	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain E 497–608（112 aa） Fragment：RESIDUES 497-608	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 5.9；1.2-1.28M AMMONIUM SULPHATE, 100MM AMMONIUM ACETATE, PH 5.9, 30MM DTT	Resolution 2.80 Å R-free 0.273
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 240 PDB declaration: 240-meric	Chain A 1–823（823 aa） Chain D 1–823（823 aa） Chain G 1–823（823 aa） Chain J 1–823（823 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain A 1–823（823 aa） Chain D 1–823（823 aa） Chain G 1–823（823 aa） Chain J 1–823（823 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain A 1–823（823 aa） Chain D 1–823（823 aa） Chain G 1–823（823 aa） Chain J 1–823（823 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7WOO Cryo-EM structure of the inner ring protomer of the Saccharomyces cerevisiae nuclear pore complex Deposited 2022-01-22	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric	Chain I 1–823（823 aa） Chain L 1–823（823 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.71 Å
7WOT Cryo-EM structure of the inner ring monomer of the Saccharomyces cerevisiae nuclear pore complex Deposited 2022-01-22	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric	Chain I 1–823（823 aa） Chain L 1–823（823 aa） Chain U 1–823（823 aa） Chain X 1–823（823 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.73 Å
8TJ5 Inner spoke ring of the yeast NPC Deposited 2023-07-20	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 46 PDB declaration: 46-meric	Chain A 1–823（823 aa） Chain D 1–823（823 aa） Chain G 1–823（823 aa） Chain J 1–823（823 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20mM HEPES,50mM Potassium acetate,20mM NaCl,2mM MgCl2,1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.60 Å

5 other PDB entries and 9 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	NSP1_YEAST
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain A； PDBConstruct 1–823； UniProt 1–823 Author chain D； PDBConstruct 1–823； UniProt 1–823 Author chain G； PDBConstruct 1–823； UniProt 1–823 Author chain J； PDBConstruct 1–823； UniProt 1–823 Author chain y； PDBConstruct 1–823； UniProt 1–823 Author chain z； PDBConstruct 1–823； UniProt 1–823

Nucleoporin NUP57

OrganismNot specified

UniProt P48837

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain B； UniProt 1–541 Chain E； UniProt 1–541 Chain H； UniProt 1–541 Chain K； UniProt 1–541	Not recorded	Nucleoporin NUP170 × 16 （P38181） Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NIC96 × 32 （P34077） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP120 × 16 （P35729） Nucleoporin NUP85 × 16 （P46673） Nucleoporin 145c × 16 （P49687） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP84 × 16 （P52891） Nucleoporin NUP133 × 16 （P36161） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain B； UniProt 1–541 Chain E； UniProt 1–541 Chain H； UniProt 1–541 Chain K； UniProt 1–541	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain B； UniProt 1–541 Chain E； UniProt 1–541 Chain H； UniProt 1–541 Chain K； UniProt 1–541	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 240 PDB declaration: 240-meric	Chain B 1–541（541 aa） Chain E 1–541（541 aa） Chain H 1–541（541 aa） Chain K 1–541（541 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain B 1–541（541 aa） Chain E 1–541（541 aa） Chain H 1–541（541 aa） Chain K 1–541（541 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain B 1–541（541 aa） Chain E 1–541（541 aa） Chain H 1–541（541 aa） Chain K 1–541（541 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7WOO Cryo-EM structure of the inner ring protomer of the Saccharomyces cerevisiae nuclear pore complex Deposited 2022-01-22	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric	Chain H 1–541（541 aa） Chain K 1–541（541 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.71 Å
7WOT Cryo-EM structure of the inner ring monomer of the Saccharomyces cerevisiae nuclear pore complex Deposited 2022-01-22	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric	Chain H 1–541（541 aa） Chain K 1–541（541 aa） Chain T 1–541（541 aa） Chain W 1–541（541 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.73 Å
8TJ5 Inner spoke ring of the yeast NPC Deposited 2023-07-20	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 46 PDB declaration: 46-meric	Chain B 1–541（541 aa） Chain E 1–541（541 aa） Chain H 1–541（541 aa） Chain K 1–541（541 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20mM HEPES,50mM Potassium acetate,20mM NaCl,2mM MgCl2,1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.60 Å

4 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	NUP57_YEAST
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain B； PDBConstruct 1–541； UniProt 1–541 Author chain E； PDBConstruct 1–541； UniProt 1–541 Author chain H； PDBConstruct 1–541； UniProt 1–541 Author chain K； PDBConstruct 1–541； UniProt 1–541

Nucleoporin NUP49/NSP49

OrganismNot specified

UniProt Q02199

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain C； UniProt 1–472 Chain F； UniProt 1–472 Chain I； UniProt 1–472 Chain L； UniProt 1–472	Not recorded	Nucleoporin NUP170 × 16 （P38181） Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NIC96 × 32 （P34077） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP120 × 16 （P35729） Nucleoporin NUP85 × 16 （P46673） Nucleoporin 145c × 16 （P49687） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP84 × 16 （P52891） Nucleoporin NUP133 × 16 （P36161） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain C； UniProt 1–472 Chain F； UniProt 1–472 Chain I； UniProt 1–472 Chain L； UniProt 1–472	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain C； UniProt 1–472 Chain F； UniProt 1–472 Chain I； UniProt 1–472 Chain L； UniProt 1–472	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 240 PDB declaration: 240-meric	Chain C 1–472（472 aa） Chain F 1–472（472 aa） Chain I 1–472（472 aa） Chain L 1–472（472 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain C 1–472（472 aa） Chain F 1–472（472 aa） Chain I 1–472（472 aa） Chain L 1–472（472 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain C 1–472（472 aa） Chain F 1–472（472 aa） Chain I 1–472（472 aa） Chain L 1–472（472 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7WOO Cryo-EM structure of the inner ring protomer of the Saccharomyces cerevisiae nuclear pore complex Deposited 2022-01-22	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric	Chain G 1–472（472 aa） Chain J 1–472（472 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.71 Å
7WOT Cryo-EM structure of the inner ring monomer of the Saccharomyces cerevisiae nuclear pore complex Deposited 2022-01-22	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric	Chain G 1–472（472 aa） Chain J 1–472（472 aa） Chain S 1–472（472 aa） Chain V 1–472（472 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.73 Å
8TJ5 Inner spoke ring of the yeast NPC Deposited 2023-07-20	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 46 PDB declaration: 46-meric	Chain C 1–472（472 aa） Chain F 1–472（472 aa） Chain I 1–472（472 aa） Chain L 1–472（472 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20mM HEPES,50mM Potassium acetate,20mM NaCl,2mM MgCl2,1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.60 Å

4 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	NUP49_YEAST
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain C； PDBConstruct 1–472； UniProt 1–472 Author chain F； PDBConstruct 1–472； UniProt 1–472 Author chain I； PDBConstruct 1–472； UniProt 1–472 Author chain L； PDBConstruct 1–472； UniProt 1–472

Nucleoporin NUP192

OrganismNot specified

UniProt P47054

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain M； UniProt 1–1683 Chain O； UniProt 1–1683	Not recorded	Nucleoporin NUP170 × 16 （P38181） Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NIC96 × 32 （P34077） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP120 × 16 （P35729） Nucleoporin NUP85 × 16 （P46673） Nucleoporin 145c × 16 （P49687） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP84 × 16 （P52891） Nucleoporin NUP133 × 16 （P36161） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain M； UniProt 1–1683 Chain O； UniProt 1–1683	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain M； UniProt 1–1683 Chain O； UniProt 1–1683	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
4IFQ Crystal structure of Saccharomyces cerevisiae NUP192, residues 2 to 960 [ScNup192(2-960)] Deposited 2012-12-14	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 2–960（959 aa） Fragment：UNP residues 2-960	Non-standard monomer：Yes (specific site not provided by mmCIF)	SO4 SULFATE ION × 7 IOD IODIDE ION × 7	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Protein (20 mM Hepes, pH 8.0, 500 mM NaCl, 10% glycerol, 5mM DTT; Reservoir (10% PEG3350, 100mM pottasium iodide); Cryoprotection (30% PEG400 and 25% saturated ammonium sulfate), Vapor Diffusion, Sitting Drop, temperature 298K	Resolution 3.25 Å R-free 0.243
4IFQ Crystal structure of Saccharomyces cerevisiae NUP192, residues 2 to 960 [ScNup192(2-960)] Deposited 2012-12-14	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 2–960（959 aa） Fragment：UNP residues 2-960	Non-standard monomer：Yes (specific site not provided by mmCIF)	SO4 SULFATE ION × 14 IOD IODIDE ION × 14	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Protein (20 mM Hepes, pH 8.0, 500 mM NaCl, 10% glycerol, 5mM DTT; Reservoir (10% PEG3350, 100mM pottasium iodide); Cryoprotection (30% PEG400 and 25% saturated ammonium sulfate), Vapor Diffusion, Sitting Drop, temperature 298K	Resolution 3.25 Å R-free 0.243
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 240 PDB declaration: 240-meric	Chain M 1–1683（1683 aa） Chain O 1–1683（1683 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain M 1–1683（1683 aa） Chain O 1–1683（1683 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain M 1–1683（1683 aa） Chain O 1–1683（1683 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7WOO Cryo-EM structure of the inner ring protomer of the Saccharomyces cerevisiae nuclear pore complex Deposited 2022-01-22	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric	Chain F 1–1683（1683 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.71 Å
7WOT Cryo-EM structure of the inner ring monomer of the Saccharomyces cerevisiae nuclear pore complex Deposited 2022-01-22	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric	Chain F 1–1683（1683 aa） Chain R 1–1683（1683 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.73 Å
8TJ5 Inner spoke ring of the yeast NPC Deposited 2023-07-20	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 46 PDB declaration: 46-meric	Chain M 1–1683（1683 aa） Chain O 1–1683（1683 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20mM HEPES,50mM Potassium acetate,20mM NaCl,2mM MgCl2,1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.60 Å

5 other PDB entries and 8 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	NU192_YEAST
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain M； PDBConstruct 1–1683； UniProt 1–1683 Author chain O； PDBConstruct 1–1683； UniProt 1–1683

Nucleoporin NUP188

OrganismNot specified

UniProt P52593

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain N； UniProt 1–1655 Chain P； UniProt 1–1655	Not recorded	Nucleoporin NUP170 × 16 （P38181） Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NIC96 × 32 （P34077） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP120 × 16 （P35729） Nucleoporin NUP85 × 16 （P46673） Nucleoporin 145c × 16 （P49687） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP84 × 16 （P52891） Nucleoporin NUP133 × 16 （P36161） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain N； UniProt 1–1655 Chain P； UniProt 1–1655	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain N； UniProt 1–1655 Chain P； UniProt 1–1655	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 136 PDB declaration: 136-meric	Chain X 1–1655（1655 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric	Chain X 1–1655（1655 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric	Chain X 1–1655（1655 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 240 PDB declaration: 240-meric	Chain N 1–1655（1655 aa） Chain P 1–1655（1655 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain N 1–1655（1655 aa） Chain P 1–1655（1655 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain N 1–1655（1655 aa） Chain P 1–1655（1655 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7WO9 Cryo-EM structure of full-length Nup188 Deposited 2022-01-20	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–1655（1655 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.81 Å
7WOO Cryo-EM structure of the inner ring protomer of the Saccharomyces cerevisiae nuclear pore complex Deposited 2022-01-22	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric	Chain E 1–1655（1655 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.71 Å
7WOT Cryo-EM structure of the inner ring monomer of the Saccharomyces cerevisiae nuclear pore complex Deposited 2022-01-22	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric	Chain E 1–1655（1655 aa） Chain Q 1–1655（1655 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.73 Å
8TJ5 Inner spoke ring of the yeast NPC Deposited 2023-07-20	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 46 PDB declaration: 46-meric	Chain N 1–1655（1655 aa） Chain P 1–1655（1655 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20mM HEPES,50mM Potassium acetate,20mM NaCl,2mM MgCl2,1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.60 Å

6 other PDB entries and 10 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	NU188_YEAST
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain N； PDBConstruct 1–1655； UniProt 1–1655 Author chain P； PDBConstruct 1–1655； UniProt 1–1655

Nucleoporin NIC96

OrganismNot specified

UniProt P34077

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain Q； UniProt 1–839 Chain R； UniProt 1–839 Chain S； UniProt 1–839 Chain T； UniProt 1–839	Not recorded	Nucleoporin NUP170 × 16 （P38181） Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP120 × 16 （P35729） Nucleoporin NUP85 × 16 （P46673） Nucleoporin 145c × 16 （P49687） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP84 × 16 （P52891） Nucleoporin NUP133 × 16 （P36161） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain Q； UniProt 1–839 Chain R； UniProt 1–839 Chain S； UniProt 1–839 Chain T； UniProt 1–839	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain Q； UniProt 1–839 Chain R； UniProt 1–839 Chain S； UniProt 1–839 Chain T； UniProt 1–839	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
2QX5 Structure of nucleoporin Nic96 Deposited 2007-08-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 186–839（654 aa） Fragment：residues 186-839	Not recorded	CL CHLORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.2；289 K；18% PEG 3350, 0.1M Bis Tris propane, 0.2M Potassium Thiocyanate, 1mM Cetyltrimethylammonium bromide, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 2.50 Å R-free 0.285
2QX5 Structure of nucleoporin Nic96 Deposited 2007-08-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 186–839（654 aa） Fragment：residues 186-839	Not recorded	CL CHLORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.2；289 K；18% PEG 3350, 0.1M Bis Tris propane, 0.2M Potassium Thiocyanate, 1mM Cetyltrimethylammonium bromide, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 2.50 Å R-free 0.285
2RFO Crystral Structure of the nucleoporin Nic96 Deposited 2007-10-01	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 189–839（651 aa） Fragment：UNP residues 189-839	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；3-10% PEG3350, 0.1M BisTris pH6.5, 0.05M lithium sulfate, 3% 1,6-hexandiole, 0.01mM DTE, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.60 Å R-free 0.285
2RFO Crystral Structure of the nucleoporin Nic96 Deposited 2007-10-01	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 189–839（651 aa） Fragment：UNP residues 189-839	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；3-10% PEG3350, 0.1M BisTris pH6.5, 0.05M lithium sulfate, 3% 1,6-hexandiole, 0.01mM DTE, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.60 Å R-free 0.285
6X07 Nic96 from S. cerevisiae bound by VHH-SAN12 Deposited 2020-05-15	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 186–839（654 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；8% PEG 8,000 and 0.1M tri-sodium citrate pH 5	Resolution 2.10 Å R-free 0.245
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 240 PDB declaration: 240-meric	Chain Q 1–839（839 aa） Chain R 1–839（839 aa） Chain S 1–839（839 aa） Chain T 1–839（839 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain Q 1–839（839 aa） Chain R 1–839（839 aa） Chain S 1–839（839 aa） Chain T 1–839（839 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain Q 1–839（839 aa） Chain R 1–839（839 aa） Chain S 1–839（839 aa） Chain T 1–839（839 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7WOO Cryo-EM structure of the inner ring protomer of the Saccharomyces cerevisiae nuclear pore complex Deposited 2022-01-22	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric	Chain A 1–839（839 aa） Chain Z 1–839（839 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.71 Å
7WOT Cryo-EM structure of the inner ring monomer of the Saccharomyces cerevisiae nuclear pore complex Deposited 2022-01-22	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric	Chain A 1–839（839 aa） Chain M 1–839（839 aa） Chain N 1–839（839 aa） Chain Z 1–839（839 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.73 Å
8TJ5 Inner spoke ring of the yeast NPC Deposited 2023-07-20	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 46 PDB declaration: 46-meric	Chain Q 1–839（839 aa） Chain R 1–839（839 aa） Chain S 1–839（839 aa） Chain T 1–839（839 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20mM HEPES,50mM Potassium acetate,20mM NaCl,2mM MgCl2,1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.60 Å

7 other PDB entries and 11 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	NIC96_YEAST
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain Q； PDBConstruct 1–839； UniProt 1–839 Author chain R； PDBConstruct 1–839； UniProt 1–839 Author chain S； PDBConstruct 1–839； UniProt 1–839 Author chain T； PDBConstruct 1–839； UniProt 1–839

Nucleoporin NUP53

OrganismNot specified

UniProt Q03790

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain U； UniProt 1–475 Chain W； UniProt 1–475	Not recorded	Nucleoporin NUP170 × 16 （P38181） Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NIC96 × 32 （P34077） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP120 × 16 （P35729） Nucleoporin NUP85 × 16 （P46673） Nucleoporin 145c × 16 （P49687） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP84 × 16 （P52891） Nucleoporin NUP133 × 16 （P36161） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain U； UniProt 1–475 Chain W； UniProt 1–475	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain U； UniProt 1–475 Chain W； UniProt 1–475	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3W3Y Crystal structure of Kap121p bound to Nup53p Deposited 2012-12-28	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 401–448（48 aa） Fragment：UNP residues 401-448	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.1M HEPES, 10% 2-propanol, 24% PEG 20000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.80 Å R-free 0.297
5UAZ Crystal structure of the yeast nucleoporin Deposited 2016-12-20	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 247–355（109 aa） Chain B 247–355（109 aa）	Not recorded	EDO 1,2-ETHANEDIOL × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；20% (wt/vol) PEG 3350 0.2 M Ammonium sulfate	Resolution 1.75 Å R-free 0.199
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 240 PDB declaration: 240-meric	Chain U 1–475（475 aa） Chain W 1–475（475 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain U 1–475（475 aa） Chain W 1–475（475 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain U 1–475（475 aa） Chain W 1–475（475 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
8TJ5 Inner spoke ring of the yeast NPC Deposited 2023-07-20	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 46 PDB declaration: 46-meric	Chain U 1–475（475 aa） Chain W 1–475（475 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20mM HEPES,50mM Potassium acetate,20mM NaCl,2mM MgCl2,1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.60 Å

4 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	NUP53_YEAST
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain U； PDBConstruct 1–475； UniProt 1–475 Author chain W； PDBConstruct 1–475； UniProt 1–475

Nucleoporin ASM4

OrganismNot specified

UniProt Q05166

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain V； UniProt 1–528 Chain X； UniProt 1–528	Not recorded	Nucleoporin NUP170 × 16 （P38181） Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NIC96 × 32 （P34077） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP120 × 16 （P35729） Nucleoporin NUP85 × 16 （P46673） Nucleoporin 145c × 16 （P49687） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP84 × 16 （P52891） Nucleoporin NUP133 × 16 （P36161） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain V； UniProt 1–528 Chain X； UniProt 1–528	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain V； UniProt 1–528 Chain X； UniProt 1–528	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 240 PDB declaration: 240-meric	Chain V 1–528（528 aa） Chain X 1–528（528 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain V 1–528（528 aa） Chain X 1–528（528 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
7N85 Inner ring spoke from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric	Chain V 1–528（528 aa） Chain X 1–528（528 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 7.60 Å
8TJ5 Inner spoke ring of the yeast NPC Deposited 2023-07-20	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 46 PDB declaration: 46-meric	Chain V 1–528（528 aa） Chain X 1–528（528 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20mM HEPES,50mM Potassium acetate,20mM NaCl,2mM MgCl2,1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 6.60 Å

2 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	NUP59_YEAST
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain V； PDBConstruct 1–528； UniProt 1–528 Author chain X； PDBConstruct 1–528； UniProt 1–528

Nucleoporin NUP82

OrganismNot specified

UniProt P40368

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain u； UniProt 1–713 Chain v； UniProt 1–713	Not recorded	Nucleoporin NUP170 × 16 （P38181） Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NIC96 × 32 （P34077） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP120 × 16 （P35729） Nucleoporin NUP85 × 16 （P46673） Nucleoporin 145c × 16 （P49687） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP84 × 16 （P52891） Nucleoporin NUP133 × 16 （P36161） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain u； UniProt 1–713 Chain v； UniProt 1–713	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain u； UniProt 1–713 Chain v； UniProt 1–713	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3PBP Structure of the yeast heterotrimeric Nup82-Nup159-Nup116 nucleoporin complex Deposited 2010-10-20	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain A 1–452（452 aa） Fragment：N-terminal domain (NTD), UNP residues 1-452	Mutation：C396S Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.6；298 K；PEG 400, sodium cacodylate, lithium sulfate, 2,5-hexanediol, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K	Resolution 2.60 Å R-free 0.272
3PBP Structure of the yeast heterotrimeric Nup82-Nup159-Nup116 nucleoporin complex Deposited 2010-10-20	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain D 1–452（452 aa） Fragment：N-terminal domain (NTD), UNP residues 1-452	Mutation：C396S Non-standard monomer：Yes (specific site not provided by mmCIF)	PGE TRIETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.6；298 K；PEG 400, sodium cacodylate, lithium sulfate, 2,5-hexanediol, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K	Resolution 2.60 Å R-free 0.272
3PBP Structure of the yeast heterotrimeric Nup82-Nup159-Nup116 nucleoporin complex Deposited 2010-10-20	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain G 1–452（452 aa） Fragment：N-terminal domain (NTD), UNP residues 1-452	Mutation：C396S Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.6；298 K；PEG 400, sodium cacodylate, lithium sulfate, 2,5-hexanediol, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K	Resolution 2.60 Å R-free 0.272
3PBP Structure of the yeast heterotrimeric Nup82-Nup159-Nup116 nucleoporin complex Deposited 2010-10-20	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain J 1–452（452 aa） Fragment：N-terminal domain (NTD), UNP residues 1-452	Mutation：C396S Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.6；298 K；PEG 400, sodium cacodylate, lithium sulfate, 2,5-hexanediol, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K	Resolution 2.60 Å R-free 0.272
3TKN Structure of the Nup82-Nup159-Nup98 heterotrimer Deposited 2011-08-28	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain A 1–452（452 aa） Fragment：UNP residues 1-452	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；18.5% PEG3350, 100 mM potassium thiocyanate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K	Resolution 3.40 Å R-free 0.285
3TKN Structure of the Nup82-Nup159-Nup98 heterotrimer Deposited 2011-08-28	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain D 1–452（452 aa） Fragment：UNP residues 1-452	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；18.5% PEG3350, 100 mM potassium thiocyanate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K	Resolution 3.40 Å R-free 0.285
3TKN Structure of the Nup82-Nup159-Nup98 heterotrimer Deposited 2011-08-28	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain G 1–452（452 aa） Fragment：UNP residues 1-452	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；18.5% PEG3350, 100 mM potassium thiocyanate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K	Resolution 3.40 Å R-free 0.285

2 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	NUP82_YEAST
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain u； PDBConstruct 1–713； UniProt 1–713 Author chain v； PDBConstruct 1–713； UniProt 1–713

Nucleoporin NUP159

OrganismNot specified

UniProt P40477

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain w； UniProt 1–1460 Chain x； UniProt 1–1460	Not recorded	Nucleoporin NUP170 × 16 （P38181） Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NIC96 × 32 （P34077） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP120 × 16 （P35729） Nucleoporin NUP85 × 16 （P46673） Nucleoporin 145c × 16 （P49687） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP84 × 16 （P52891） Nucleoporin NUP133 × 16 （P36161） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain w； UniProt 1–1460 Chain x； UniProt 1–1460	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain w； UniProt 1–1460 Chain x； UniProt 1–1460	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
1XIP Crystal Structure of the N-terminal Domain of Nup159 Deposited 2004-09-21	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 2–387（386 aa） Fragment：N-terminal domain	Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；292 K；PEG3350, ammonium acetate, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 292K	Resolution 2.50 Å R-free 0.249
3PBP Structure of the yeast heterotrimeric Nup82-Nup159-Nup116 nucleoporin complex Deposited 2010-10-20	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain C 1425–1460（36 aa） Fragment：Tail, UNP residues 1425-1460	Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.6；298 K；PEG 400, sodium cacodylate, lithium sulfate, 2,5-hexanediol, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K	Resolution 2.60 Å R-free 0.272
3PBP Structure of the yeast heterotrimeric Nup82-Nup159-Nup116 nucleoporin complex Deposited 2010-10-20	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain F 1425–1460（36 aa） Fragment：Tail, UNP residues 1425-1460	Non-standard monomer：Yes (specific site not provided by mmCIF)	PGE TRIETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.6；298 K；PEG 400, sodium cacodylate, lithium sulfate, 2,5-hexanediol, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K	Resolution 2.60 Å R-free 0.272
3PBP Structure of the yeast heterotrimeric Nup82-Nup159-Nup116 nucleoporin complex Deposited 2010-10-20	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain I 1425–1460（36 aa） Fragment：Tail, UNP residues 1425-1460	Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.6；298 K；PEG 400, sodium cacodylate, lithium sulfate, 2,5-hexanediol, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K	Resolution 2.60 Å R-free 0.272
3PBP Structure of the yeast heterotrimeric Nup82-Nup159-Nup116 nucleoporin complex Deposited 2010-10-20	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain L 1425–1460（36 aa） Fragment：Tail, UNP residues 1425-1460	Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.6；298 K；PEG 400, sodium cacodylate, lithium sulfate, 2,5-hexanediol, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K	Resolution 2.60 Å R-free 0.272
3RRM S. cerevisiae dbp5 l327v bound to nup159, gle1 h337r, ip6 and adp Deposited 2011-04-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain C 2–387（386 aa） Fragment：unp residues 2-387	Not recorded	ADP ADENOSINE-5'-DIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 MG MAGNESIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；20% PEG 3350, 200 mM KOAc, 20 mM sarcosine, 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 0.5 mM IP6, 10 mM MgCl2, 1 mM ADP, 5% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 2.90 Å R-free 0.261
3TKN Structure of the Nup82-Nup159-Nup98 heterotrimer Deposited 2011-08-28	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain B 1425–1460（36 aa） Fragment：UNP residues 1425-1460	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；18.5% PEG3350, 100 mM potassium thiocyanate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K	Resolution 3.40 Å R-free 0.285
3TKN Structure of the Nup82-Nup159-Nup98 heterotrimer Deposited 2011-08-28	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain E 1425–1460（36 aa） Fragment：UNP residues 1425-1460	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；18.5% PEG3350, 100 mM potassium thiocyanate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K	Resolution 3.40 Å R-free 0.285
3TKN Structure of the Nup82-Nup159-Nup98 heterotrimer Deposited 2011-08-28	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain H 1425–1460（36 aa） Fragment：UNP residues 1425-1460	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；18.5% PEG3350, 100 mM potassium thiocyanate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K	Resolution 3.40 Å R-free 0.285
4DS1 The Structure of a Yeast Dyn2-Nup159 Complex and the Molecular Basis for the Dynein Light Chain - Nuclear Pore Interaction Deposited 2012-02-17	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain B 1116–1126（11 aa） Fragment：UNP residues 1116-1126 Chain D 1116–1126（11 aa） Fragment：UNP residues 1116-1126	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；0.3 M Ammonium acetate, 5% methyl pentanediol (v/v), 35% PEG 4000 (w/v), pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 1.85 Å R-free 0.187

5 other PDB entries and 10 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	NU159_YEAST
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain w； PDBConstruct 1–1460； UniProt 1–1460 Author chain x； PDBConstruct 1–1460； UniProt 1–1460

Nucleoporin NUP120

OrganismNot specified

UniProt P35729

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain a； UniProt 1–1037 Chain h； UniProt 1–1037	Not recorded	Nucleoporin NUP170 × 16 （P38181） Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NIC96 × 32 （P34077） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP85 × 16 （P46673） Nucleoporin 145c × 16 （P49687） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP84 × 16 （P52891） Nucleoporin NUP133 × 16 （P36161） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain a； UniProt 1–1037 Chain h； UniProt 1–1037	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain a； UniProt 1–1037 Chain h； UniProt 1–1037	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3F7F Structure of Nup120 Deposited 2008-11-08	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–729（729 aa） Fragment：UNP residues 1-729	Mutation：S207C	HG MERCURY (II) ION × 3	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；Succinic acid, PEG 3350, Sodium bromide, Ethylene glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 2.60 Å R-free 0.254
3F7F Structure of Nup120 Deposited 2008-11-08	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 1–729（729 aa） Fragment：UNP residues 1-729	Mutation：S207C	HG MERCURY (II) ION × 3	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；Succinic acid, PEG 3350, Sodium bromide, Ethylene glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 2.60 Å R-free 0.254
3F7F Structure of Nup120 Deposited 2008-11-08	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain C 1–729（729 aa） Fragment：UNP residues 1-729	Mutation：S207C	HG MERCURY (II) ION × 3	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；Succinic acid, PEG 3350, Sodium bromide, Ethylene glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 2.60 Å R-free 0.254
3F7F Structure of Nup120 Deposited 2008-11-08	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain D 1–729（729 aa） Fragment：UNP residues 1-729	Mutation：S207C	HG MERCURY (II) ION × 3	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；Succinic acid, PEG 3350, Sodium bromide, Ethylene glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 2.60 Å R-free 0.254
3F7F Structure of Nup120 Deposited 2008-11-08	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1–729（729 aa） Fragment：UNP residues 1-729 Chain B 1–729（729 aa） Fragment：UNP residues 1-729	Mutation：S207C Mutation：S207C	HG MERCURY (II) ION × 6	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；Succinic acid, PEG 3350, Sodium bromide, Ethylene glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 2.60 Å R-free 0.254
3F7F Structure of Nup120 Deposited 2008-11-08	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 6 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain C 1–729（729 aa） Fragment：UNP residues 1-729 Chain D 1–729（729 aa） Fragment：UNP residues 1-729	Mutation：S207C Mutation：S207C	HG MERCURY (II) ION × 6	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；Succinic acid, PEG 3350, Sodium bromide, Ethylene glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 2.60 Å R-free 0.254
3H7N Structure of Nup120 Deposited 2009-04-27	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–729（729 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Sodium citrate (tribasic dihydrate), Potassium thiocynate, PEG 2000 MME, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K	Resolution 3.00 Å R-free 0.274
3H7N Structure of Nup120 Deposited 2009-04-27	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 1–729（729 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Sodium citrate (tribasic dihydrate), Potassium thiocynate, PEG 2000 MME, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K	Resolution 3.00 Å R-free 0.274
3H7N Structure of Nup120 Deposited 2009-04-27	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain C 1–729（729 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Sodium citrate (tribasic dihydrate), Potassium thiocynate, PEG 2000 MME, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K	Resolution 3.00 Å R-free 0.274
3H7N Structure of Nup120 Deposited 2009-04-27	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain D 1–729（729 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Sodium citrate (tribasic dihydrate), Potassium thiocynate, PEG 2000 MME, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K	Resolution 3.00 Å R-free 0.274
3HXR Nucleoporin Nup120 from S.cerevisiae (aa 1-757) Deposited 2009-06-21	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–757（757 aa） Fragment：UNP residues 1-757	Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；15% PEG3350, 0.2M KSCN, 0.1M Tris-HCl pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 3.00 Å R-free 0.299
4XMM Structure of the yeast coat nucleoporin complex, space group C2 Deposited 2015-01-14	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain E 2–1037（1036 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；PEG 20000, ethanol, MES	Resolution 7.38 Å R-free 0.353
4XMN Structure of the yeast coat nucleoporin complex, space group P212121 Deposited 2015-01-14	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric	Chain E 1–1037（1037 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；PEG 20000, ethanol, MES	Resolution 7.60 Å R-free 0.347
6X06 Nup120 (aa1-757) from S. cerevisiae bound by VHH-SAN11 Deposited 2020-05-15	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 1–757（757 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；19% PEG 8,000, 0.1M magnesium chloride, 0.1M Tris/HCl pH 8.5	Resolution 4.27 Å R-free 0.359
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 136 PDB declaration: 136-meric	Chain a 1–1037（1037 aa） Chain l 1–1037（1037 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric	Chain a 1–1037（1037 aa） Chain l 1–1037（1037 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric	Chain a 1–1037（1037 aa） Chain l 1–1037（1037 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
8TIE Double nuclear outer ring of Nup84-complexes from the yeast NPC Deposited 2023-07-19	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric	Chain a 1–1037（1037 aa） Chain l 1–1037（1037 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20mM HEPES,50mM Potassium acetate,20mM NaCl,2mM MgCl2,1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 8.10 Å

8 other PDB entries and 18 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	NU120_YEAST
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain a； PDBConstruct 1–1037； UniProt 1–1037 Author chain h； PDBConstruct 1–1037； UniProt 1–1037

Nucleoporin NUP85

OrganismNot specified

UniProt P46673

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain b； UniProt 1–744 Chain i； UniProt 1–744	Not recorded	Nucleoporin NUP170 × 16 （P38181） Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NIC96 × 32 （P34077） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP120 × 16 （P35729） Nucleoporin 145c × 16 （P49687） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP84 × 16 （P52891） Nucleoporin NUP133 × 16 （P36161） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain b； UniProt 1–744 Chain i； UniProt 1–744	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain b； UniProt 1–744 Chain i； UniProt 1–744	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3EWE Crystal Structure of the Nup85/Seh1 Complex Deposited 2008-10-14	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 1–564（564 aa） Fragment：UNP residues 1-564	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 8.5；289 K；18% PEG 3350, 0.1M Bis Tris propane, 0.2M Sodium Citrate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 3.50 Å R-free 0.369
3EWE Crystal Structure of the Nup85/Seh1 Complex Deposited 2008-10-14	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain D 1–564（564 aa） Fragment：UNP residues 1-564	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 8.5；289 K；18% PEG 3350, 0.1M Bis Tris propane, 0.2M Sodium Citrate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 3.50 Å R-free 0.369
3EWE Crystal Structure of the Nup85/Seh1 Complex Deposited 2008-10-14	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain B 1–564（564 aa） Fragment：UNP residues 1-564 Chain D 1–564（564 aa） Fragment：UNP residues 1-564	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 8.5；289 K；18% PEG 3350, 0.1M Bis Tris propane, 0.2M Sodium Citrate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 3.50 Å R-free 0.369
3F3F Crystal structure of the nucleoporin pair Nup85-Seh1, space group P21 Deposited 2008-10-30	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain C 1–570（570 aa） Fragment：UNP residues 1-570 Chain D 1–570（570 aa） Fragment：UNP residues 1-570 Chain G 1–570（570 aa） Fragment：UNP residues 1-570 Chain H 1–570（570 aa） Fragment：UNP residues 1-570	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.1；298 K；PEG 10000, MES buffer, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.90 Å R-free 0.265
3F3G Crystal structure of the nucleoporin pair Nup85-Seh1, space group P212121 Deposited 2008-10-30	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain C 1–570（570 aa） Fragment：UNP residues 1-570 Chain D 1–570（570 aa） Fragment：UNP residues 1-570 Chain G 1–570（570 aa） Fragment：UNP residues 1-570 Chain H 1–570（570 aa） Fragment：UNP residues 1-570	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；Sodium citrate, Sodium chloride, Tris-HCl buffer, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.75 Å R-free 0.272
3F3P Crystal structure of the nucleoporin pair Nup85-Seh1, space group P21212 Deposited 2008-10-31	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain C 1–570（570 aa） Fragment：UNP residues 1-570 Chain D 1–570（570 aa） Fragment：UNP residues 1-570	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；PEG 3350, Tacsimate pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.20 Å R-free 0.281
3F3P Crystal structure of the nucleoporin pair Nup85-Seh1, space group P21212 Deposited 2008-10-31	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain G 1–570（570 aa） Fragment：UNP residues 1-570 Chain H 1–570（570 aa） Fragment：UNP residues 1-570	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；PEG 3350, Tacsimate pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.20 Å R-free 0.281
3F3P Crystal structure of the nucleoporin pair Nup85-Seh1, space group P21212 Deposited 2008-10-31	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain K 1–570（570 aa） Fragment：UNP residues 1-570 Chain L 1–570（570 aa） Fragment：UNP residues 1-570	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；PEG 3350, Tacsimate pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.20 Å R-free 0.281
4XMM Structure of the yeast coat nucleoporin complex, space group C2 Deposited 2015-01-14	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain D 44–744（701 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；PEG 20000, ethanol, MES	Resolution 7.38 Å R-free 0.353
4XMN Structure of the yeast coat nucleoporin complex, space group P212121 Deposited 2015-01-14	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric	Chain D 73–743（671 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；PEG 20000, ethanol, MES	Resolution 7.60 Å R-free 0.347
6X08 Nup85-Seh1 from S. cerevisiae bound by VHH-SAN2 Deposited 2020-05-15	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain B 1–564（564 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；1M ammonium sulfate, di-sodium succinate pH 5.5, and the addition of 4% 1-propanol	Resolution 4.19 Å R-free 0.346
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 136 PDB declaration: 136-meric	Chain b 1–744（744 aa） Chain m 1–744（744 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric	Chain b 1–744（744 aa） Chain m 1–744（744 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric	Chain b 1–744（744 aa） Chain m 1–744（744 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
8TIE Double nuclear outer ring of Nup84-complexes from the yeast NPC Deposited 2023-07-19	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric	Chain b 1–744（744 aa） Chain m 1–744（744 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20mM HEPES,50mM Potassium acetate,20mM NaCl,2mM MgCl2,1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 8.10 Å

9 other PDB entries and 15 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	NUP85_YEAST
Isoform	—
PDB entities	15
Chains and sequence ranges	Author chain b； PDBConstruct 1–744； UniProt 1–744 Author chain i； PDBConstruct 1–744； UniProt 1–744

Nucleoporin 145c

OrganismNot specified

UniProt P49687

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain c； UniProt 606–1317 Chain j； UniProt 606–1317	Not recorded	Nucleoporin NUP170 × 16 （P38181） Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NIC96 × 32 （P34077） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP120 × 16 （P35729） Nucleoporin NUP85 × 16 （P46673） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP84 × 16 （P52891） Nucleoporin NUP133 × 16 （P36161） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain c； UniProt 606–1317 Chain j； UniProt 606–1317	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain c； UniProt 606–1317 Chain j； UniProt 606–1317	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3BG0 Architecture of a Coat for the Nuclear Pore Membrane Deposited 2007-11-23	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 731–1158（428 aa） Fragment：Nucleoporin NUP145C Chain C 731–1158（428 aa） Fragment：Nucleoporin NUP145C Chain F 731–1158（428 aa） Fragment：Nucleoporin NUP145C Chain G 731–1158（428 aa） Fragment：Nucleoporin NUP145C	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.7；294 K；16 % (w/v) PEG 3,350, 400 mM NaCl, 100 mM Na-K tartrate, and 100 mM BIS-TRIS, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K	Resolution 3.15 Å R-free 0.294
3BG0 Architecture of a Coat for the Nuclear Pore Membrane Deposited 2007-11-23	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain B 731–1158（428 aa） Fragment：Nucleoporin NUP145C Chain C 731–1158（428 aa） Fragment：Nucleoporin NUP145C	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.7；294 K；16 % (w/v) PEG 3,350, 400 mM NaCl, 100 mM Na-K tartrate, and 100 mM BIS-TRIS, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K	Resolution 3.15 Å R-free 0.294
3BG0 Architecture of a Coat for the Nuclear Pore Membrane Deposited 2007-11-23	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain F 731–1158（428 aa） Fragment：Nucleoporin NUP145C Chain G 731–1158（428 aa） Fragment：Nucleoporin NUP145C	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.7；294 K；16 % (w/v) PEG 3,350, 400 mM NaCl, 100 mM Na-K tartrate, and 100 mM BIS-TRIS, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K	Resolution 3.15 Å R-free 0.294
3BG0 Architecture of a Coat for the Nuclear Pore Membrane Deposited 2007-11-23	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 731–1158（428 aa） Fragment：Nucleoporin NUP145C	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.7；294 K；16 % (w/v) PEG 3,350, 400 mM NaCl, 100 mM Na-K tartrate, and 100 mM BIS-TRIS, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K	Resolution 3.15 Å R-free 0.294
3BG0 Architecture of a Coat for the Nuclear Pore Membrane Deposited 2007-11-23	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain C 731–1158（428 aa） Fragment：Nucleoporin NUP145C	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.7；294 K；16 % (w/v) PEG 3,350, 400 mM NaCl, 100 mM Na-K tartrate, and 100 mM BIS-TRIS, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K	Resolution 3.15 Å R-free 0.294
3BG0 Architecture of a Coat for the Nuclear Pore Membrane Deposited 2007-11-23	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain F 731–1158（428 aa） Fragment：Nucleoporin NUP145C	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.7；294 K；16 % (w/v) PEG 3,350, 400 mM NaCl, 100 mM Na-K tartrate, and 100 mM BIS-TRIS, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K	Resolution 3.15 Å R-free 0.294
3BG0 Architecture of a Coat for the Nuclear Pore Membrane Deposited 2007-11-23	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 7 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain G 731–1158（428 aa） Fragment：Nucleoporin NUP145C	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.7；294 K；16 % (w/v) PEG 3,350, 400 mM NaCl, 100 mM Na-K tartrate, and 100 mM BIS-TRIS, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K	Resolution 3.15 Å R-free 0.294
3BG1 Architecture of a Coat for the Nuclear Pore Membrane Deposited 2007-11-23	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 731–1158（428 aa） Fragment：Nucleoporin NUP145C Chain C 731–1158（428 aa） Fragment：Nucleoporin NUP145C Chain F 731–1158（428 aa） Fragment：Nucleoporin NUP145C Chain G 731–1158（428 aa） Fragment：Nucleoporin NUP145C	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.7；294 K；16 % (w/v) PEG 3,350, 400 mM NaCl, 100 mM Na-K tartrate, and 100 mM BIS-TRIS, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K	Resolution 3.00 Å R-free 0.294
3BG1 Architecture of a Coat for the Nuclear Pore Membrane Deposited 2007-11-23	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain B 731–1158（428 aa） Fragment：Nucleoporin NUP145C Chain C 731–1158（428 aa） Fragment：Nucleoporin NUP145C	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.7；294 K；16 % (w/v) PEG 3,350, 400 mM NaCl, 100 mM Na-K tartrate, and 100 mM BIS-TRIS, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K	Resolution 3.00 Å R-free 0.294
3BG1 Architecture of a Coat for the Nuclear Pore Membrane Deposited 2007-11-23	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain F 731–1158（428 aa） Fragment：Nucleoporin NUP145C Chain G 731–1158（428 aa） Fragment：Nucleoporin NUP145C	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.7；294 K；16 % (w/v) PEG 3,350, 400 mM NaCl, 100 mM Na-K tartrate, and 100 mM BIS-TRIS, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K	Resolution 3.00 Å R-free 0.294
3BG1 Architecture of a Coat for the Nuclear Pore Membrane Deposited 2007-11-23	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 731–1158（428 aa） Fragment：Nucleoporin NUP145C	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.7；294 K；16 % (w/v) PEG 3,350, 400 mM NaCl, 100 mM Na-K tartrate, and 100 mM BIS-TRIS, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K	Resolution 3.00 Å R-free 0.294
3BG1 Architecture of a Coat for the Nuclear Pore Membrane Deposited 2007-11-23	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain C 731–1158（428 aa） Fragment：Nucleoporin NUP145C	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.7；294 K；16 % (w/v) PEG 3,350, 400 mM NaCl, 100 mM Na-K tartrate, and 100 mM BIS-TRIS, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K	Resolution 3.00 Å R-free 0.294
3BG1 Architecture of a Coat for the Nuclear Pore Membrane Deposited 2007-11-23	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain F 731–1158（428 aa） Fragment：Nucleoporin NUP145C	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.7；294 K；16 % (w/v) PEG 3,350, 400 mM NaCl, 100 mM Na-K tartrate, and 100 mM BIS-TRIS, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K	Resolution 3.00 Å R-free 0.294
3BG1 Architecture of a Coat for the Nuclear Pore Membrane Deposited 2007-11-23	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 7 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain G 731–1158（428 aa） Fragment：Nucleoporin NUP145C	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.7；294 K；16 % (w/v) PEG 3,350, 400 mM NaCl, 100 mM Na-K tartrate, and 100 mM BIS-TRIS, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K	Resolution 3.00 Å R-free 0.294
3IKO Crystal structure of the heterotrimeric Sec13-Nup145C-Nup84 nucleoporin complex Deposited 2009-08-06	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain B 731–1158（428 aa） Fragment：UNP residues 731-1158	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG 20000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 3.20 Å R-free 0.273
3IKO Crystal structure of the heterotrimeric Sec13-Nup145C-Nup84 nucleoporin complex Deposited 2009-08-06	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain E 731–1158（428 aa） Fragment：UNP residues 731-1158	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG 20000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 3.20 Å R-free 0.273
3IKO Crystal structure of the heterotrimeric Sec13-Nup145C-Nup84 nucleoporin complex Deposited 2009-08-06	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain H 731–1158（428 aa） Fragment：UNP residues 731-1158	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG 20000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 3.20 Å R-free 0.273
3JRO NUP84-NUP145C-SEC13 edge element of the NPC lattice Deposited 2009-09-08	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 714–1160（447 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.7；295 K；1.15M sodium malonate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 295K	Resolution 4.00 Å R-free 0.329
3JRP SEC13 with NUP145C (AA109-179) insertion blade Deposited 2009-09-08	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain A 714–784（71 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.3；289 K；0.1M Tris, 22% PEG 4000, 0.25 M LiCl, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 2.60 Å R-free 0.254
3KEP Crystal structure of the autoproteolytic domain from the nuclear pore complex component NUP145 from Saccharomyces cerevisiae Deposited 2009-10-26	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 442–605（164 aa） Fragment：residues 442 to 605	Non-standard monomer：Yes (specific site not provided by mmCIF)	EDO 1,2-ETHANEDIOL × 6	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；294 K；100mM HEPES, 25% PEG 3350, 200mM NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K	Resolution 1.82 Å R-free 0.244
3KEP Crystal structure of the autoproteolytic domain from the nuclear pore complex component NUP145 from Saccharomyces cerevisiae Deposited 2009-10-26	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 442–605（164 aa） Fragment：residues 442 to 605	Non-standard monomer：Yes (specific site not provided by mmCIF)	EDO 1,2-ETHANEDIOL × 11	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；294 K；100mM HEPES, 25% PEG 3350, 200mM NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K	Resolution 1.82 Å R-free 0.244
3KES Crystal structure of the autoproteolytic domain from the nuclear pore complex component NUP145 from Saccharomyces cerevisiae in the Hexagonal, P61 space group Deposited 2009-10-26	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 442–605（164 aa） Fragment：residues 442 to 605	Non-standard monomer：Yes (specific site not provided by mmCIF)	EDO 1,2-ETHANEDIOL × 6	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；294 K；1600mM tri-sodium citrate dehydrate, VAPOR DIFFUSION, SITTING DROP, temperature 294K	Resolution 2.10 Å R-free 0.230
3KES Crystal structure of the autoproteolytic domain from the nuclear pore complex component NUP145 from Saccharomyces cerevisiae in the Hexagonal, P61 space group Deposited 2009-10-26	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 442–605（164 aa） Fragment：residues 442 to 605	Non-standard monomer：Yes (specific site not provided by mmCIF)	EDO 1,2-ETHANEDIOL × 16	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；294 K；1600mM tri-sodium citrate dehydrate, VAPOR DIFFUSION, SITTING DROP, temperature 294K	Resolution 2.10 Å R-free 0.230
4XMM Structure of the yeast coat nucleoporin complex, space group C2 Deposited 2015-01-14	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 680–1317（638 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；PEG 20000, ethanol, MES	Resolution 7.38 Å R-free 0.353
4XMN Structure of the yeast coat nucleoporin complex, space group P212121 Deposited 2015-01-14	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric	Chain B 680–1317（638 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；PEG 20000, ethanol, MES	Resolution 7.60 Å R-free 0.347
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 136 PDB declaration: 136-meric	Chain c 606–1317（712 aa） Chain n 606–1317（712 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric	Chain c 606–1317（712 aa） Chain n 606–1317（712 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric	Chain c 606–1317（712 aa） Chain n 606–1317（712 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å

10 other PDB entries and 28 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	NU145_YEAST
Isoform	—
PDB entities	16
Chains and sequence ranges	Author chain c； PDBConstruct 1–712； UniProt 606–1317 Author chain j； PDBConstruct 1–712； UniProt 606–1317

Protein transport protein SEC13

OrganismNot specified

UniProt Q04491

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain d； UniProt 1–297 Chain k； UniProt 1–297	Not recorded	Nucleoporin NUP170 × 16 （P38181） Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NIC96 × 32 （P34077） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP120 × 16 （P35729） Nucleoporin NUP85 × 16 （P46673） Nucleoporin 145c × 16 （P49687） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP84 × 16 （P52891） Nucleoporin NUP133 × 16 （P36161） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain d； UniProt 1–297 Chain k； UniProt 1–297	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain d； UniProt 1–297 Chain k； UniProt 1–297	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
2PM6 Crystal Structure of yeast Sec13/31 edge element of the COPII vesicular coat, native version Deposited 2007-04-20	Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain B 1–297（297 aa） Chain D 1–297（297 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；15% PEG 4000, 10% DMSO, 6% dioxanie, 0.1M Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 2.45 Å R-free 0.301
2PM7 Crystal structure of yeast Sec13/31 edge element of the COPII vesicular coat, selenomethionine version Deposited 2007-04-20	Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain B 1–297（297 aa） Chain D 1–297（297 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；15% PEG 4000, 10% DMSO, 6% dioxane, 0.1M Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 2.35 Å R-free 0.298
2PM9 Crystal structure of yeast Sec13/31 vertex element of the COPII vesicular coat Deposited 2007-04-20	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 1–297（297 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；295 K；8% PEG 3350, 0.1M tri-sodium citrate, 10mM manganese chloride, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K	Resolution 3.30 Å R-free 0.305
3IKO Crystal structure of the heterotrimeric Sec13-Nup145C-Nup84 nucleoporin complex Deposited 2009-08-06	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain A 1–297（297 aa） Fragment：UNP residues 1-297	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG 20000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 3.20 Å R-free 0.273
3IKO Crystal structure of the heterotrimeric Sec13-Nup145C-Nup84 nucleoporin complex Deposited 2009-08-06	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain D 1–297（297 aa） Fragment：UNP residues 1-297	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG 20000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 3.20 Å R-free 0.273
3IKO Crystal structure of the heterotrimeric Sec13-Nup145C-Nup84 nucleoporin complex Deposited 2009-08-06	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain G 1–297（297 aa） Fragment：UNP residues 1-297	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG 20000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 3.20 Å R-free 0.273
3JRO NUP84-NUP145C-SEC13 edge element of the NPC lattice Deposited 2009-09-08	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 1–297（297 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.7；295 K；1.15M sodium malonate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 295K	Resolution 4.00 Å R-free 0.329
3JRP SEC13 with NUP145C (AA109-179) insertion blade Deposited 2009-09-08	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–297（297 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.3；289 K；0.1M Tris, 22% PEG 4000, 0.25 M LiCl, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 2.60 Å R-free 0.254
3MZK Sec13/Sec16 complex, S.cerevisiae Deposited 2010-05-12	Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain A 1–297（297 aa） Chain D 1–297（297 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；289 K；0.1mM bis-Tris propane, 0.2M NaBr, 12% polyethylene glycol 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 2.69 Å R-free 0.243
3MZL Sec13/Sec31 edge element, loop deletion mutant Deposited 2010-05-12	Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain A 1–297（297 aa） Chain C 1–297（297 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；289 K；0.1mM bis-Tris propane, 0.2M sodium citrate, 16% polyethylene glycol 3350, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 2.80 Å R-free 0.300
3MZL Sec13/Sec31 edge element, loop deletion mutant Deposited 2010-05-12	Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain E 1–297（297 aa） Chain G 1–297（297 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；289 K；0.1mM bis-Tris propane, 0.2M sodium citrate, 16% polyethylene glycol 3350, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 2.80 Å R-free 0.300
4BZJ The structure of the COPII coat assembled on membranes Deposited 2013-07-26	Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain B 2–292（291 aa） Chain F 2–292（291 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer HEPES, 50 MM KOAC, 1.2 MM MGCL2；pH 6.8；HEPES, 50 MM KOAC, 1.2 MM MGCL2 cryo-EM vitrification conditions Cryogen ETHANE；ETHANE; HOMEMADE PLUNGER	Resolution 40.00 Å
4BZK The structure of the COPII coat assembled on membranes Deposited 2013-07-26	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain B 1–297（297 aa） Chain F 1–297（297 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer HEPES, 50 MM KOAC, 1.2 MM MGCL2；pH 6.8；HEPES, 50 MM KOAC, 1.2 MM MGCL2 cryo-EM vitrification conditions Cryogen ETHANE；ETHANE; HOMEMADE PLUNGER	Resolution 40.00 Å
4XMM Structure of the yeast coat nucleoporin complex, space group C2 Deposited 2015-01-14	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–297（297 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；PEG 20000, ethanol, MES	Resolution 7.38 Å R-free 0.353
4XMN Structure of the yeast coat nucleoporin complex, space group P212121 Deposited 2015-01-14	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric	Chain A 2–297（296 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；PEG 20000, ethanol, MES	Resolution 7.60 Å R-free 0.347
6ZG5 COPII on membranes, outer coat right-handed rod, class1 Deposited 2020-06-18	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain B 1–297（297 aa） Chain F 1–297（297 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 6.8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 40.00 Å
6ZG6 COPII on membranes, outer coat vertex Deposited 2020-06-18	Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain B 1–297（297 aa） Chain D 1–297（297 aa） Chain F 1–297（297 aa） Chain H 1–297（297 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 6.8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 12.00 Å
6ZL0 COPII on membranes, outer coat left-handed rod Deposited 2020-06-30	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain B 1–297（297 aa） Chain D 1–297（297 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 6.8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 40.00 Å
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 136 PDB declaration: 136-meric	Chain d 1–297（297 aa） Chain o 1–297（297 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric	Chain d 1–297（297 aa） Chain o 1–297（297 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric	Chain d 1–297（297 aa） Chain o 1–297（297 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
8ADL Cryo-EM structure of the SEA complex Deposited 2022-07-08	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric	Chain H 1–297（297 aa） Chain P 1–297（297 aa）	Not recorded	ZN ZINC ION × 28	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.95 Å
8TIE Double nuclear outer ring of Nup84-complexes from the yeast NPC Deposited 2023-07-19	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric	Chain d 1–297（297 aa） Chain o 1–297（297 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20mM HEPES,50mM Potassium acetate,20mM NaCl,2mM MgCl2,1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 8.10 Å
9H5K Cryo-EM structure of the SEAC-EGOC supercomplex Deposited 2024-10-22	Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric	Chain H 1–297（297 aa） Chain P 1–297（297 aa）	Not recorded	ZN ZINC ION × 28 MG MAGNESIUM ION × 2 AF3 ALUMINUM FLUORIDE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å

19 other PDB entries and 24 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	SEC13_YEAST
Isoform	—
PDB entities	17
Chains and sequence ranges	Author chain d； PDBConstruct 1–297； UniProt 1–297 Author chain k； PDBConstruct 1–297； UniProt 1–297

Nucleoporin SEH1

OrganismNot specified

UniProt P53011

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain e； UniProt 1–349 Chain l； UniProt 1–349	Not recorded	Nucleoporin NUP170 × 16 （P38181） Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NIC96 × 32 （P34077） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP120 × 16 （P35729） Nucleoporin NUP85 × 16 （P46673） Nucleoporin 145c × 16 （P49687） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin NUP84 × 16 （P52891） Nucleoporin NUP133 × 16 （P36161） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain e； UniProt 1–349 Chain l； UniProt 1–349	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain e； UniProt 1–349 Chain l； UniProt 1–349	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3EWE Crystal Structure of the Nup85/Seh1 Complex Deposited 2008-10-14	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 1–349（349 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 8.5；289 K；18% PEG 3350, 0.1M Bis Tris propane, 0.2M Sodium Citrate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 3.50 Å R-free 0.369
3EWE Crystal Structure of the Nup85/Seh1 Complex Deposited 2008-10-14	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain C 1–349（349 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 8.5；289 K；18% PEG 3350, 0.1M Bis Tris propane, 0.2M Sodium Citrate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 3.50 Å R-free 0.369
3EWE Crystal Structure of the Nup85/Seh1 Complex Deposited 2008-10-14	Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain A 1–349（349 aa） Chain C 1–349（349 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 8.5；289 K；18% PEG 3350, 0.1M Bis Tris propane, 0.2M Sodium Citrate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K	Resolution 3.50 Å R-free 0.369
3F3F Crystal structure of the nucleoporin pair Nup85-Seh1, space group P21 Deposited 2008-10-30	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–349（349 aa） Chain B 1–349（349 aa） Chain E 1–349（349 aa） Chain F 1–349（349 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.1；298 K；PEG 10000, MES buffer, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.90 Å R-free 0.265
3F3G Crystal structure of the nucleoporin pair Nup85-Seh1, space group P212121 Deposited 2008-10-30	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain A 1–349（349 aa） Chain B 1–349（349 aa） Chain E 1–349（349 aa） Chain F 1–349（349 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；Sodium citrate, Sodium chloride, Tris-HCl buffer, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.75 Å R-free 0.272
3F3P Crystal structure of the nucleoporin pair Nup85-Seh1, space group P21212 Deposited 2008-10-31	Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain A 1–349（349 aa） Chain B 1–349（349 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；PEG 3350, Tacsimate pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.20 Å R-free 0.281
3F3P Crystal structure of the nucleoporin pair Nup85-Seh1, space group P21212 Deposited 2008-10-31	Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain E 1–349（349 aa） Chain F 1–349（349 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；PEG 3350, Tacsimate pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.20 Å R-free 0.281
3F3P Crystal structure of the nucleoporin pair Nup85-Seh1, space group P21212 Deposited 2008-10-31	Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain I 1–349（349 aa） Chain J 1–349（349 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；PEG 3350, Tacsimate pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.20 Å R-free 0.281
4XMM Structure of the yeast coat nucleoporin complex, space group C2 Deposited 2015-01-14	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain C 1–349（349 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；PEG 20000, ethanol, MES	Resolution 7.38 Å R-free 0.353
6X08 Nup85-Seh1 from S. cerevisiae bound by VHH-SAN2 Deposited 2020-05-15	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain A 1–349（349 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；1M ammonium sulfate, di-sodium succinate pH 5.5, and the addition of 4% 1-propanol	Resolution 4.19 Å R-free 0.346
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 136 PDB declaration: 136-meric	Chain e 1–349（349 aa） Chain p 1–349（349 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric	Chain e 1–349（349 aa） Chain p 1–349（349 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric	Chain e 1–349（349 aa） Chain p 1–349（349 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
8ADL Cryo-EM structure of the SEA complex Deposited 2022-07-08	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 22 PDB declaration: 22-meric	Chain D 1–349（349 aa） Chain E 1–349（349 aa） Chain F 1–349（349 aa） Chain L 1–349（349 aa） Chain M 1–349（349 aa） Chain N 1–349（349 aa）	Not recorded	ZN ZINC ION × 28	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.95 Å
9H5K Cryo-EM structure of the SEAC-EGOC supercomplex Deposited 2024-10-22	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric	Chain D 1–349（349 aa） Chain E 1–349（349 aa） Chain F 1–349（349 aa） Chain L 1–349（349 aa） Chain M 1–349（349 aa） Chain N 1–349（349 aa）	Not recorded	ZN ZINC ION × 28 MG MAGNESIUM ION × 2 AF3 ALUMINUM FLUORIDE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 4	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.20 Å

9 other PDB entries and 15 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	SEH1_YEAST
Isoform	—
PDB entities	18
Chains and sequence ranges	Author chain e； PDBConstruct 1–349； UniProt 1–349 Author chain l； PDBConstruct 1–349； UniProt 1–349

Nucleoporin NUP84

OrganismNot specified

UniProt P52891

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain f； UniProt 1–726 Chain m； UniProt 1–726	Not recorded	Nucleoporin NUP170 × 16 （P38181） Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NIC96 × 32 （P34077） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP120 × 16 （P35729） Nucleoporin NUP85 × 16 （P46673） Nucleoporin 145c × 16 （P49687） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP133 × 16 （P36161） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain f； UniProt 1–726 Chain m； UniProt 1–726	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain f； UniProt 1–726 Chain m； UniProt 1–726	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP133 × 2 （P36161） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3IKO Crystal structure of the heterotrimeric Sec13-Nup145C-Nup84 nucleoporin complex Deposited 2009-08-06	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain C 1–460（460 aa） Fragment：UNP residues 1-460	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG 20000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 3.20 Å R-free 0.273
3IKO Crystal structure of the heterotrimeric Sec13-Nup145C-Nup84 nucleoporin complex Deposited 2009-08-06	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain F 1–460（460 aa） Fragment：UNP residues 1-460	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG 20000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 3.20 Å R-free 0.273
3IKO Crystal structure of the heterotrimeric Sec13-Nup145C-Nup84 nucleoporin complex Deposited 2009-08-06	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain I 1–460（460 aa） Fragment：UNP residues 1-460	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG 20000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 3.20 Å R-free 0.273
3JRO NUP84-NUP145C-SEC13 edge element of the NPC lattice Deposited 2009-09-08	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric	Chain C 1–424（424 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.7；295 K；1.15M sodium malonate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 295K	Resolution 4.00 Å R-free 0.329
4XMM Structure of the yeast coat nucleoporin complex, space group C2 Deposited 2015-01-14	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric	Chain F 1–451（451 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；PEG 20000, ethanol, MES	Resolution 7.38 Å R-free 0.353
4XMN Structure of the yeast coat nucleoporin complex, space group P212121 Deposited 2015-01-14	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric	Chain F 2–451（450 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；PEG 20000, ethanol, MES	Resolution 7.60 Å R-free 0.347
6X02 Nup84-Nup133 (aa521-1157) from S. cerevisiae bound by VHH-SAN8 Deposited 2020-05-15	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain A 1–726（726 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；6% PEG 8,000, 30% ethylene glycol, 0.1M imidazole titrated with MES pH 6.5, 15mM sodium nitrate, 15mM sodium phosphate dibasic, 15mM ammonium sulfate	Resolution 6.38 Å R-free 0.324
6X03 Nup84-Nup133 (aa521-1157) from S. cerevisiae bound by VHH-SAN8 and VHH-SAN9 Deposited 2020-05-15	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain A 1–726（726 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；6% PEG 8,000, 30% ethylene glycol, 0.1M imidazole titrated with MES pH 6.5, 15mM sodium nitrate, 15mM sodium phosphate dibasic, 15mM ammonium sulfate	Resolution 7.30 Å R-free 0.353
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 136 PDB declaration: 136-meric	Chain f 1–726（726 aa） Chain q 1–726（726 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric	Chain f 1–726（726 aa） Chain q 1–726（726 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric	Chain f 1–726（726 aa） Chain q 1–726（726 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
8TIE Double nuclear outer ring of Nup84-complexes from the yeast NPC Deposited 2023-07-19	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric	Chain f 1–726（726 aa） Chain q 1–726（726 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5；20mM HEPES,50mM Potassium acetate,20mM NaCl,2mM MgCl2,1mM DTT cryo-EM vitrification conditions Cryogen ETHANE	Resolution 8.10 Å

8 other PDB entries and 12 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	NUP84_YEAST
Isoform	—
PDB entities	19
Chains and sequence ranges	Author chain f； PDBConstruct 1–726； UniProt 1–726 Author chain m； PDBConstruct 1–726； UniProt 1–726

Nucleoporin NUP133

OrganismNot specified

UniProt P36161

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain g； UniProt 1–1157 Chain n； UniProt 1–1157	Not recorded	Nucleoporin NUP170 × 16 （P38181） Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NIC96 × 32 （P34077） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP120 × 16 （P35729） Nucleoporin NUP85 × 16 （P46673） Nucleoporin 145c × 16 （P49687） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP84 × 16 （P52891） Dynein light chain 1, cytoplasmic × 48 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain g； UniProt 1–1157 Chain n； UniProt 1–1157	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain g； UniProt 1–1157 Chain n； UniProt 1–1157	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Dynein light chain 1, cytoplasmic × 6 （Q02647）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
3KFO Crystal structure of the C-terminal domain from the nuclear pore complex component NUP133 from Saccharomyces cerevisiae Deposited 2009-10-27	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 881–1157（277 aa） Fragment：C-terminal domain, residues 881-1157	Non-standard monomer：Yes (specific site not provided by mmCIF)	GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；294 K；20% PEG 3350, 200mM Potassium Thiocyanate, VAPOR DIFFUSION, SITTING DROP, temperature 294K	Resolution 1.90 Å R-free 0.245
6X02 Nup84-Nup133 (aa521-1157) from S. cerevisiae bound by VHH-SAN8 Deposited 2020-05-15	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain B 521–1157（637 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；6% PEG 8,000, 30% ethylene glycol, 0.1M imidazole titrated with MES pH 6.5, 15mM sodium nitrate, 15mM sodium phosphate dibasic, 15mM ammonium sulfate	Resolution 6.38 Å R-free 0.324
6X03 Nup84-Nup133 (aa521-1157) from S. cerevisiae bound by VHH-SAN8 and VHH-SAN9 Deposited 2020-05-15	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain B 521–1157（637 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；6% PEG 8,000, 30% ethylene glycol, 0.1M imidazole titrated with MES pH 6.5, 15mM sodium nitrate, 15mM sodium phosphate dibasic, 15mM ammonium sulfate	Resolution 7.30 Å R-free 0.353
6X04 Nup133 (aa55-481) from S. cerevisiae bound by VHH-SAN5 Deposited 2020-05-15	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric	Chain A 55–481（427 aa） Chain C 55–481（427 aa） Chain E 55–481（427 aa） Chain G 55–481（427 aa） Chain I 55–481（427 aa） Chain K 55–481（427 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；15% PEG MME 2,000, 0.1M potassium chloride, 0.1M Tris/HCl pH 8	Resolution 2.68 Å R-free 0.261
6X05 Nup133 (aa55-481) from S. cerevisiae bound by VHH-SAN4 Deposited 2020-05-15	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 55–481（427 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；24% PEG 3,350, 0.2M Ammonium sulfate, 0.1M Bis-Tris pH 5.5	Resolution 2.10 Å R-free 0.219
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 136 PDB declaration: 136-meric	Chain g 1–1157（1157 aa） Chain r 1–1157（1157 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric	Chain g 1–1157（1157 aa） Chain r 1–1157（1157 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å
7N84 Double nuclear outer ring from the isolated yeast NPC Deposited 2021-06-13	Different oligomeric state Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric	Chain g 1–1157（1157 aa） Chain r 1–1157（1157 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 11.60 Å

6 other PDB entries and 8 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	NU133_YEAST
Isoform	—
PDB entities	20
Chains and sequence ranges	Author chain g； PDBConstruct 1–1157； UniProt 1–1157 Author chain n； PDBConstruct 1–1157； UniProt 1–1157

Dynein light chain 1, cytoplasmic

OrganismNot specified

UniProt Q02647

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 448 PDB declaration: 448-meric（448） Consistent with protein copy count	Chain o； UniProt 1–92 Chain p； UniProt 1–92 Chain q； UniProt 1–92 Chain r； UniProt 1–92 Chain s； UniProt 1–92 Chain t； UniProt 1–92	Not recorded	Nucleoporin NUP170 × 16 （P38181） Nucleoporin NUP157 × 16 （P40064） orphans bound to Nup192 NTD × 16 Nucleoporin NSP1 × 48 （P14907） Nucleoporin NUP57 × 32 （P48837） Nucleoporin NUP49/NSP49 × 32 （Q02199） Nucleoporin NUP192 × 16 （P47054） Nucleoporin NUP188 × 16 （P52593） Nucleoporin NIC96 × 32 （P34077） Nucleoporin NUP53 × 16 （Q03790） Nucleoporin ASM4 × 16 （Q05166） Nucleoporin NUP82 × 16 （P40368） Nucleoporin NUP159 × 16 （P40477） Nucleoporin NUP120 × 16 （P35729） Nucleoporin NUP85 × 16 （P46673） Nucleoporin 145c × 16 （P49687） Protein transport protein SEC13 × 16 （Q04491） Nucleoporin SEH1 × 16 （P53011） Nucleoporin NUP84 × 16 （P52891） Nucleoporin NUP133 × 16 （P36161）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
2	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain o； UniProt 1–92 Chain p； UniProt 1–92 Chain q； UniProt 1–92 Chain r； UniProt 1–92 Chain s； UniProt 1–92 Chain t； UniProt 1–92	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å
3	Protein heterocomplex Heteromer Protein × 56 PDB declaration: 56-meric（56） Consistent with protein copy count	Chain o； UniProt 1–92 Chain p； UniProt 1–92 Chain q； UniProt 1–92 Chain r； UniProt 1–92 Chain s； UniProt 1–92 Chain t； UniProt 1–92	Not recorded	Nucleoporin NUP170 × 2 （P38181） Nucleoporin NUP157 × 2 （P40064） orphans bound to Nup192 NTD × 2 Nucleoporin NSP1 × 6 （P14907） Nucleoporin NUP57 × 4 （P48837） Nucleoporin NUP49/NSP49 × 4 （Q02199） Nucleoporin NUP192 × 2 （P47054） Nucleoporin NUP188 × 2 （P52593） Nucleoporin NIC96 × 4 （P34077） Nucleoporin NUP53 × 2 （Q03790） Nucleoporin ASM4 × 2 （Q05166） Nucleoporin NUP82 × 2 （P40368） Nucleoporin NUP159 × 2 （P40477） Nucleoporin NUP120 × 2 （P35729） Nucleoporin NUP85 × 2 （P46673） Nucleoporin 145c × 2 （P49687） Protein transport protein SEC13 × 2 （Q04491） Nucleoporin SEH1 × 2 （P53011） Nucleoporin NUP84 × 2 （P52891） Nucleoporin NUP133 × 2 （P36161）	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE-PROPANE；A custom-built vitrification device (Max Planck Institute for Biochemistry, Munich)	Resolution 37.00 Å

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 7N9F	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
4DS1 The Structure of a Yeast Dyn2-Nup159 Complex and the Molecular Basis for the Dynein Light Chain - Nuclear Pore Interaction Deposited 2012-02-17	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain A 1–92（92 aa） Chain C 1–92（92 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；0.3 M Ammonium acetate, 5% methyl pentanediol (v/v), 35% PEG 4000 (w/v), pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 1.85 Å R-free 0.187
4HT6 The Structure of a Yeast Dynein Dyn2-Pac11 Complex and Effect on Single Molecule Dynein Motor Activity Deposited 2012-10-31	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 1–92（92 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；0.4M sodium phosphate monobasic, 0.1M 1,6-hexanediol, 25% PEG 3350 (w/v), pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 1.90 Å R-free 0.212
4HT6 The Structure of a Yeast Dynein Dyn2-Pac11 Complex and Effect on Single Molecule Dynein Motor Activity Deposited 2012-10-31	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain C 1–92（92 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；0.4M sodium phosphate monobasic, 0.1M 1,6-hexanediol, 25% PEG 3350 (w/v), pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 1.90 Å R-free 0.212
4HT6 The Structure of a Yeast Dynein Dyn2-Pac11 Complex and Effect on Single Molecule Dynein Motor Activity Deposited 2012-10-31	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain E 1–92（92 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；0.4M sodium phosphate monobasic, 0.1M 1,6-hexanediol, 25% PEG 3350 (w/v), pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 1.90 Å R-free 0.212
4HT6 The Structure of a Yeast Dynein Dyn2-Pac11 Complex and Effect on Single Molecule Dynein Motor Activity Deposited 2012-10-31	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain E 1–92（92 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；0.4M sodium phosphate monobasic, 0.1M 1,6-hexanediol, 25% PEG 3350 (w/v), pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 1.90 Å R-free 0.212

2 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	DYL1_YEAST
Isoform	—
PDB entities	21
Chains and sequence ranges	Author chain o； PDBConstruct 1–92； UniProt 1–92 Author chain p； PDBConstruct 1–92； UniProt 1–92 Author chain q； PDBConstruct 1–92； UniProt 1–92 Author chain r； PDBConstruct 1–92； UniProt 1–92 Author chain s； PDBConstruct 1–92； UniProt 1–92 Author chain t； PDBConstruct 1–92； UniProt 1–92

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

Download Download SAXS (.dat) P(r) (.pr.out) CIF

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	7n9f
Deposition date deposition_date	2021-06-17
Structure title title	Structure of the in situ yeast NPC
Keywords keywords	NPC, nucleocytoplasmic transport, TRANSLOCASE; TRANSLOCASE
Experimental Method method	ELECTRON MICROSCOPY

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	— Å
Radius of gyration Rg (electron density) rg_electron	176.30 Å
Forward intensity I(0) i0	115941000000.00
Molecular weight molecular_weight	3015900.0 kDa
Excluded volume excluded_volume	3810100 Å³
Envelope volume envelope_volume	10279000 Å³
Hydration-shell volume shell_volume	532230 Å³
Envelope diameter envelope_diameter	714.4 Å
Shell Rg shell_rg	129.30 Å
Envelope Rg envelope_rg	173.70 Å
Shape Rg shape_rg	176.40 Å
Total Rg total_rg	176.00 Å
Total atoms total_atoms	212962
Residues n_residues	27071
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	474.6 Å
Rg (real space) rg_real	164.50 Å
Rg uncertainty (real space) rg_real_error	1.95 Å
I(0) (real space) i0_real	1.1020e+11
I(0) uncertainty (real space) i0_real_error	2.8700e+09
Rg (reciprocal space) rg_reciprocal	142.10 Å
I(0) (reciprocal space) i0_reciprocal	100000000000.0000
Solution quality estimate total_estimate	0.9129
Solution quality rating solution_quality	EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks	1
Primary peak position r_peak_primary	158.2 Å
Skewness Skewness skewness	0.253
Kurtosis Kurtosis kurtosis	-0.695
Angular range angular_range	— – 0.0450 Å⁻¹
Current regularization parameter α current_alpha	0.9329
Highest regularization parameter α highest_alpha	5983000000.0000
Real-space data points n_real_points	10
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.269; Oscil: 0.998; Stabil: 0.976; Sysdev: 1.000; Positv: 1.000; Valcen: 0.944; Smooth: 0.000

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (21)

Entity 1 Entity 1 polymer

Description description	Nucleoporin NUP170
Molecular weight formula_weight	169652.0 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	0,Y
Atom count atom_count	212962

MFQSFFHNNGPAAAGETFSDSRSYPLTNHQEVPRNGLNELASSATKAQQQPTHILNSYPITGSNPLMRASAMGATSGSINPNMSNMNEHIRVSGMGTSKPLDLAGKYIDHLQHKDSNTPVLDERSYYNSGVDYNFSREKNGLGAFTPFEKQDVFNIPDEILHEFSTSQTKTDMGIFPELNRCWITIDNKLILWNINNDNEYQVVDDMKHTIQKVALVRPKPNTFVPAVKHLLLISTTMELFMFAISLDKATNELSVFNTHLSVPVQGIDVIDIVSHERSGRIFFAGQASGLNIWELHYSGSDDWFNSKCSKVCLTKSALLSLLPTNMLSQIPGVDFIQALFEDNSNGNGGFSQETITQLTIDQQRGIIYSLSSKSTIRAYVITEKSLEGPMSIEPAYISRIIGTTTARAAPILGPKYLKIVKISSVAPEENNNLFLVALTVGGVRLYFNGSMGRFNIEALRLESIKFPPSSVTPEVIQQELLHQQQEQAKRSFPFFSNLMSSEPVLLKFQKKSSVLLETTKASTIISPGIFFSAVIKSSQQTHQQEKKENSSVTGTTATAGSKTVKQQPVTLQHKLFVSVPDYGILKTHGKYVENATFLETAGPVQQIIPLSGLFNATTKPQGFANEFATQYTSETLRVAVLTSTSIEIYKYRTPDEIFEDLIDNPLPFVLNYGAAEACSTALFVTCKSNKSEKLRSNALTFLTMGIPGVVDIKPVYNRYSVSTVSSLLSKPTLSTATTNLQQSITGFSKPSPANKEDFDLDDVILSPRFYGIALLITRLLRDIWGRHVFMTFTDNRVTSHAFISSSDPITPSINNLKSDEISQNRNIISKVSISKDCIEYYLSSINILNEFFITYGDSISQISAPYVLANNSNGRVIDKTEEVANQAESIAINAMIKMVQSIKEGLSFLNVLYEESEVEGFDNQYLGFKDIISFVSLDVQKDLVKLDFKDLFAPNDKTKSLIREILLSIINRNITKGASIEYTATALQERCGSFCSASDILGFRAIEHLRRAKEIGLRNYDSLNYHLKNATALLEQIVDDLSIEKLKEAVSMMLSVNYYPKSIEFLLNIANSMDKGKLACQYVANGFLENDDRKQYYDKRILVYDLVFDTLIKVDELAEKKQSSKTQNQISISNDDEVKLRQKSYEAALKYNDRLFHYHMYDWLVSQNREEKLLDIETPFILPYLMEKAGSSLKISNILWVYYSRRSKFFESAEILYRLATSNFDITLFERIEFLSRANGFCNSVSPLSQKQRIVQLASRIQDACEVAGIQGDILSLVYTDARIDSAIKDELIKTLDGKILSTSELFNDFAVPLSYHEIALFIFKIADFRDHEVIMAKWDELFQSLRMEFNNTGKKEDSMNFINLLSNVLIKIGKNVQDSEFIFPIFELFPIVCNFFYETLPKEHIVSGSIVSIFITAGVSFNKMYYILKELIETSDSDNSVFNKEMTWLIHEWYKSDRKFRDIISYNDIIHLKEYKIDNDPIEKYVKNSGNNLGICFYKE

Entity 2 Entity 2 polymer

Description description	Nucleoporin NUP157
Molecular weight formula_weight	156827.5 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	1,Z

MYSTPLKKRIDYDRETFTASASLGGNRLRNRPRDDQNNGKPNLSSRSFLSERKTRKDVLNKYGEAGNTIESELRDVTTHVKISGLTSSEPLQLASEFVQDLSFRDRNTPILDNPDYYSKGLDYNFSDEVGGLGAFTPFQRQQVTNIPDEVLSQVSNTEIKSDMGIFLELNYCWITSDNKLILWNINNSSEYHCIDEIEHTILKVKLVKPSPNTFVSSVENLLIVATLFDIYILTISFNDRTHELNIFNTGLKVNVTGFNVSNIISYERTGQIFFTGATDGVNVWELQYNCSENLFNSKSNKICLTKSNLANLLPTKLIPSIPGGKLIQKVLEGDAGTEEETISQLEVDQSRGVLHTLSTKSIVRSYLITSNGLVGPVLIDAAHIRRGMNALGVKNSPLLSNRAFKIAKIVSISMCENNDLFLAVITTTGVRLYFKGSISRRSIGSLKLDSVKFPPTSISSSLEQNKSFIIGHHPLNTHDTGPLSTQKASSTYINTTCASTIISPGIYFTCVRKRANSGELSKGITNKALLENKEEHKLYVSAPDYGILKNYGKYVENTALLDTTDEIKEIVPLTRSFNYTSTPQGYANVFASQYSAEPLKVAVLTSNALEIYCYRTPDEVFESLIENPLPFIHSYGLSEACSTALYLACKFNKSEHIKSSALAFFSAGIPGVVEIKPKSSRESGSVPPISQNLFDKSGECDGIVLSPRFYGSALLITRLFSQIWEERVFVFKRASKTEKMDAFGISITRPQVEYYLSSISVLADFFNIHRPSFVSFVPPKGSNAITASDAESIAMNALILLINSIKDALSLINVFYEDIDAFKSLLNTLMGAGGVYDSKTREYFFDLKFHDLFTPNAKTKQLIKEILIEVVNANIASGTSADYIVNVLKERFGSFCHSADILCYRAGEHLEAAQKFEMIDSKISRNHLDTAIDLYERCAENIELCELRRVVDIMVKLNYQPKTVGFLLRFADKIDKGNQAQEYVSRGCNTADPRKVFYDKRINVYTLIFEIVKSVDDYTSIEQSPSIANISIFSPASSLKKRVYSVIMNSNNRFFHYCFYDWLVANKRQDYLLRLDSQFVLPYLKERAEKSLEISNLLWFYLFKEEHFLEAADVLYALASSDFDLKLSERIECLARANGLCDSSTSFDQKPALVQLSENIHELFDIASIQDDLLNLVRNETRIDEDYRKQLTLKLNGRVLPLSDLFNDCADPLDYYEIKLRIFKVSQFKDEKVIQGEWNRLLDSMKNAPSPDVGSVGQESFLSSISNTLIRIGKTTRDTDVVFPVHFLMNKILESFIDKSSAADGSVCSMFLLAGVSHLKLYYILSRIIENSEGNVELAKKEMVWLIKDWYQSDSDLRGSIAPEQIKKLEKYDPNTDPVQDYVKDRHHGLK

Entity 3 Entity 3 polymer

Description description	orphans bound to Nup192 NTD
Molecular weight formula_weight	3081.8 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	5,6

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

Entity 4 Entity 4 polymer

Description description	Nucleoporin NSP1
Molecular weight formula_weight	86611.7 kDa
Source method src_method	nat
Copy count entity_count	6

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,D,G,J,y,z

MNFNTPQQNKTPFSFGTANNNSNTTNQNSSTGAGAFGTGQSTFGFNNSAPNNTNNANSSITPAFGSNNTGNTAFGNSNPTSNVFGSNNSTTNTFGSNSAGTSLFGSSSAQQTKSNGTAGGNTFGSSSLFNNSTNSNTTKPAFGGLNFGGGNNTTPSSTGNANTSNNLFGATANANKPAFSFGATTNDDKKTEPDKPAFSFNSSVGNKTDAQAPTTGFSFGSQLGGNKTVNEAAKPSLSFGSGSAGANPAGASQPEPTTNEPAKPALSFGTATSDNKTTNTTPSFSFGAKSDENKAGATSKPAFSFGAKPEEKKDDNSSKPAFSFGAKSNEDKQDGTAKPAFSFGAKPAEKNNNETSKPAFSFGAKSDEKKDGDASKPAFSFGAKPDENKASATSKPAFSFGAKPEEKKDDNSSKPAFSFGAKSNEDKQDGTAKPAFSFGAKPAEKNNNETSKPAFSFGAKSDEKKDGDASKPAFSFGAKSDEKKDSDSSKPAFSFGTKSNEKKDSGSSKPAFSFGAKPDEKKNDEVSKPAFSFGAKANEKKESDESKSAFSFGSKPTGKEEGDGAKAAISFGAKPEEQKSSDTSKPAFTFGAQKDNEKKTEESSTGKSTADVKSSDSLKLNSKPVELKPVSLDNKTLDDLVTKWTNQLTESASHFEQYTKKINSWDQVLVKGGEQISQLYSDAVMAEHSQNKIDQSLQYIERQQDELENFLDNFETKTEALLSDVVSTSSGAAANNNDQKRQQAYKTAQTLDENLNSLSSNLSSLIVEINNVSNTFNKTTNIDINNEDENIQLIKILNSHFDALRSLDDNSTSLEKQINSIKK

Entity 5 Entity 5 polymer

Description description	Nucleoporin NUP57
Molecular weight formula_weight	57547.1 kDa
Source method src_method	nat
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B,E,H,K

MFGFSGSNNGFGNKPAGSTGFSFGQNNNNTNTQPSASGFGFGGSQPNSGTATTGGFGANQATNTFGSNQQSSTGGGLFGNKPALGSLGSSSTTASGTTATGTGLFGQQTAQPQQSTIGGGLFGNKPTTTTGGLFGNSAQNNSTTSGGLFGNKVGSTGSLMGGNSTQNTSNMNAGGLFGAKPQNTTATTGGLFGSKPQGSTTNGGLFGSGTQNNNTLGGGGLFGQSQQPQTNTAPGLGNTVSTQPSFAWSKPSTGSNLQQQQQQQIQVPLQQTQAIAQQQQLSNYPQQIQEQVLKCKESWDPNTTKTKLRAFVYNKVNETEAILYTKPGHVLQEEWDQAMEKKPSPQTIPIQIYGFEGLNQRNQVQTENVAQARIILNHILEKSTQLQQKHELDTASRILKAQSRNVEIEKRILKLGTQLATLKNRGLPLGIAEEKMWSQFQTLLQRSEDPAGLGKTNELWARLAILKERAKNISSQLDSKLMVFNDDTKNQDSMSKGTGEESNDRINKIVEILTNQQRGITYLNEVLEKDAAIVKKYKNKT

Entity 6 Entity 6 polymer

Description description	Nucleoporin NUP49/NSP49
Molecular weight formula_weight	49174.8 kDa
Source method src_method	nat
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C,F,I,L

MFGLNKASSTPAGGLFGQASGASTGNANTGFSFGGTQTGQNTGPSTGGLFGAKPAGSTGGLGASFGQQQQQSQTNAFGGSATTGGGLFGNKPNNTANTGGGLFGANSNSNSGSLFGSNNAQTSRGLFGNNNTNNINNSSSGMNNASAGLFGSKPAGGTSLFGNTSTSSAPAQNQGMFGAKPAGTSLFGNNAGNTTTGGGLFGSKPTGATSLFGSSNNNNNNNNSNNIMSASGGLFGNQQQQLQQQPQMQCALQNLSQLPITPMTRISELPPQIRQEIEQLDQYIQKQVQISHHLKADTIDHDELIDSIPRDVAYLLKSESATSQYLKQDLKKISSFKSLIDEDLLDTQTFSVLLQQLLTPGSKISSNDLDKFFQKKIHLYEKKLEDYCRILSDIETAVNGIDTDLFGAPNNPNSTAITADLGSSEAENLLQLKTGLAAIVSTVIEEFTLFMDIAERIAVLHQKTKTLASLSI

Entity 7 Entity 7 polymer

Description description	Nucleoporin NUP192
Molecular weight formula_weight	191718.1 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	M,O

MKWSAIPFQTLYRSIESGEFDFDLFKEVLPDLQNLNLNTDKLKNNASRSQLEKGEIELSDGSTFKVNQEFIFEAISLSDELNLDEIVACELILSGDTTANNGKVQYFLRRQYILQIVSFIVNCFHEDTELYQELIKNGALVSNILSAFKFIHTQLSEIKQQINKAQILENYNALFQQNIKFRRDFLLREYDILSQILYGLVDKGAIMKNKDFILSLLHHVSELDSNDFFIIYYTPAFFHLFASLRVLPDADVKLLHSQFMKDLKDDSIYTKPVKVALIFIFFAYFIGWCKEDPKRRADTMDFKTDVDEPMTSAVELGAIEQILIFAADTSIVEQDKSMELFYDIRSLLERHIPRLIPKQLLDDEKIFSQTTNSTYNPASATDNMSGRGLWNPSYPGMMSTTGTARLNSMPNNVNEYSYTTIVLSDQTQEFFLSSFDDVLQTIITDCAFLLTKIKDAEEDSLLSGEDLTLDDISLKADLERFFLSIYFFYASRPEYSCTFWSDKESNAYGFIEWCSRCNDNLMRSCFYLMVSSLSFGPENALNVYHYFGENSSISWKNIAQCLSDYTKKISNFNSSLHKRQQFSESTHNDIDSTAVALEEGLNEEAVIFLSSLLTLVGSVTYQVDEDVKSSLSKVFSDVLFEFTKINTPLVGAAFKVISNLVPKLESSRTKFWSFLDSLIFKDSSLNYSSESYRNAFTNVLTKYSDVLGFLQLFHNLISIHSRENNSEYMVFGKLAFPTRLGQGYRKVGIWPYFDYIFNDILAHVDQIVDIRNKRAVQLPILKIIYTGLCSFDYSVILNSIPAAANLDALVDCENFFNYVQECPAIPIFNYIFTEKIYKSIFNVVDVGVDQLSIELEGGKNQAELLQLAVKIINKVLDYQETYVEELFPIVKKHGKTDYFLPKNYSLHGLRSFYDAIFFNIPLVAHLGLYVGVDDQILATNSLRILAKLSERSNGSVASLSKRNKLLTIFDSVDESARIKDAFITQLESSITDAGVLALKLELLDFLTSNLSNYSRTMTISHLLLGFQVSNVISLGPNLATFISSGTSLLDSLISVLEASLNSITKDNIDYAPMRLATAALEIILKLCRNPLTSGLLYSYLIKENFFERIMILDPQVTRFTTWNGSPFDNSTEEKCKNFIESESVGAFLSFLAYRNYWTQYLGLFIHKISFSGTKSEVLTYVNYLISNTMYSVRLFSFLDPLNYGNICEPKETLSIFTNVPLNLEQVTLNKYCSGNIYDFHKMENLMRLIKRVRAESLHSNSFSLTVSKEQFLKDADVECIKAKSHFTNIISRNKALELNLSVLHSWVQLVQIIVTDGKLEPSTRSNFILEVFGTIIPKISDYIEFNITFSEELVSLAVFLFDIYNRDRKLITDKGTVDGRLYQLFKTCIQGINSPLSSVALRSDFYILANHYLSRVLSDQVGSEKVLQDLRLGSKKLVEIIWNDVVYGEGTSRVTGILLLDSLIQLANRSKENFILDSLMKTTRLLLIIRSLKNTDALLNSTTEHINIDDLLYELTAFKATVFFLIRVAETRGGASALIENNLFRIIAELSFLKVDPDLGLDLMFDEVYVQNSKFLKVNVTLDNPLLVDKDANGVSLFELIVPIFQLISAVLVSMGSSNKAVVQTVKGLLNTYKRLVIGIFKRDLLREKEDKKNSSDPNNQSLNEMVKLIVMLCTLTGYQNND

Entity 8 Entity 8 polymer

Description description	Nucleoporin NUP188
Molecular weight formula_weight	188753.3 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	N,P

MATPSFGNSSPQLTFTHVANFMNDAAADVSAVDAKQLAQIRQFLKANKTNLIESLNTIRQNVTSSGDHNKLRSTIANLLQINVDNDPFFAQSEDLSHAVEFFMSERSSRLHIVYSLLVNPDIDLETYSFIDNDRFNVVGKLISIISSVIQNYDIITASSLAHDYNNDQDMFTIVSLVQLKKFSDLKFILQILQILNLMILNTKVPVDIVNQWFLQYQNQFVEFCRNINSTDKSIDTSSLQLYKFQNFQDLSYLSETLISRISSLFTITTILILGLNTSIAQFDIQSPLYMDTETFDTVNSALENDVATNIVNEDPIFHPMIHYSWSFILYYRRALQSSESFDDSDITKFALFAESHDVLQKLNTLSEILSFDPVYTTVITVFLEFSLNFIPITASTSRVFAKIISKAPEQFIENFLTNDTFEKKLSIIKAKLPLLNESLIPLINLALIDTEFANFELKDICSFAVTKSSLNDLDYDLIADTITNSSSSSDIIVPDLIELKSDLLVAPPLENENSNCLLSIPKSTKGKILTIKQQQQQQQQQNGQQPPTTSNLIIFLYKFNGWSLVGRILQNLLHSYMEKGTQLDDLQHELMISIIKLVTNVVDPKTSIEKSSEILSYLSNSLDTSASTINGASIIQVIFEIFEISLQRKDYTSIVQCCEFMTMLTPNYLHLVSSYLNKSDLLDKYGKTGLSNMILGSVELSTGDYTFTIQLLKLTKVFIRESLSLKNIHISKRSKIDIINKLILHAIHIFESYYNWKYNNFLQKFEIAFHLTLIFYDVLHDVFTINPHQKDQLIISSSANKLLQLFLTPMDSIDLAPNTLTNILISPLNTTTKILGDKILGNLYSKVMNNSFKLCTLLIAIRGSNRDLKPSNLEKLLFINSSKLVDVYTLPSYVHFKVQIIELLSYLVEAPWNDDYPFLLSFLGEAKSMAFLKEVLSDLSSPVQDWNLLRSLYIFFTTLLESKQDGLSILFLTGQFASNKKINDESSIDKKSSILTVLQKNSLLLDSTPEEVSCKLLETITYVLNTWTNSKIFIKDPKFVNSLLAKLKDSKKLFQKKENLTRDETVSLIKKYKLISRIVEIFALCIYNSTDSNSEILNFLNQEDLFELVHHFFQIDGFNKTFHDELNLKFKEKWPSLELQSFQKIPLSRINENENFGYDIPLLDIVLKADRSWNEPSKSQTNFKEEITDASLNLQYVNYEISTAKAWGALITTFVKRSTVPLNDGFVDLVEHFLKLNIDFGSDKQMFTQIYLERIELSFYILYSFKLSGKLLKEEKIIELMNKIFTIFKSGEIDFIKNIGKSLKNNFYRPLLRSVLVLLELVSSGDRFIELISDQLLEFFELVFSKGVYLILSEILCQINKCSTRGLSTDHTTQIVNLEDNTQDLLLLLSLFKKITNVNPSKNFNVILASSLNEVGTLKVILNLYSSAHLIRINDEPILGQITLTFISELCSIEPIAAKLINSGLYSVLLESPLSVAIQQGDIKPEFSPRLHNIWSNGLLSIVLLLLSQFGIKVLPETCLFVSYFGKQIKSTIYNWGDNKLAVSSSLIKETNQLVLLQKMLNLLNYQELFIQPKNSDDQQEAVELVIGLDSEHDKKRLSAALSKFLTHPKYLNSRIIPTTLEEQQQLEDESSRLEFVKGISRDIKALQDSLFKDV

Entity 9 Entity 9 polymer

Description description	Nucleoporin NIC96
Molecular weight formula_weight	96291.6 kDa
Source method src_method	nat
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Q,R,S,T

MLETLRGNKLHSGTSKGANKKLNELLESSDNLPSASSELGSIQVSINELRRRVFQLRSKNKASKDYTKAHYLLANSGLSFEDVDAFIKDLQTNQFLEPNPPKIIESEELEFYIRTKKEENILMSIEQLLNGATKDFDNFINHNLNLDWAQHKNEVMKNFGILIQDKKTVDHKKSISSLDPKLPSWGNKGNNILNSNESRLNVNENNILREKFENYARIVFQFNNSRQANGNFDIANEFISILSSANGTRNAQLLESWKILESMKSKDINIVEVGKQYLEQQFLQYTDNLYKKNMNEGLATNVNKIKSFIDTKLKKADKSWKISNLTVINGVPIWALIFYLLRAGLIKEALQVLVENKANIKKVEQSFLTYFKAYASSKDHGLPVEYSTKLHTEYNQHIKSSLDGDPYRLAVYKLIGRCDLSRKNIPAVTLSIEDWLWMHLMLIKEKDAENDPVYERYSLEDFQNIIISYGPSRFSNYYLQTLLLSGLYGLAIDYTYTFSEMDAVHLAIGLASLKLFKIDSSTRLTKKPKRDIRFANILANYTKSFRYSDPRVAVEYLVLITLNEGPTDVELCHEALRELVLETKEFTVLLGKIGRDGARIPGVIEERQPLLHVRDEKEFLHTITEQAARRADEDGRIYDSILLYQLAEEYDIVITLVNSLLSDTLSASDLDQPLVGPDDNSETNPVLLARRMASIYFDNAGISRQIHVKNKEICMLLLNISSIRELYFNKQWQETLSQMELLDLLPFSDELSARKKAQDFSNLDDNIVKNIPNLLIITLSCISNMIHILNESKYQSSTKGQQIDSLKNVARQCMIYAGMIQYRMPRETYSTLINIDVSL

Entity 10 Entity 10 polymer

Description description	Nucleoporin NUP53
Molecular weight formula_weight	52688.7 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	U,W

MADLQKQENSSRFTNVSVIAPESQGQHEQQKQQEQLEQQKQPTGLLKGLNGFPSAPQPLFMEDPPSTVSGELNDNPAWFNNPRKRAIPNSIIKRSNGQSLSPVRSDSADVPAFSNSNGFNNVTFGSKKDPRILKNVSPNDNNSANNNAHSSDLGTVVFDSNEAPPKTSLADWQKEDGIFSSKTDNIEDPNLSSNITFDGKPTATPSPFRPLEKTSRILNFFDKNTKTTPNTASSEASAGSKEGASTNWDDHAIIIFGYPETIANSIILHFANFGEILEDFRVIKDFKKLNSKNMSKSPSLTAQKYPIYTGDGWVKLTYKSELSKSRALQENGIIMNGTLIGCVSYSPAALKQLASLKKSEEIINNKTSSQTSLSSKDLSNYRKTEGIFEKAKAKAVTSKVRNAEFKVSKNSTSFKNPRRLEIKDGRSLFLRNRGKIHSGVLSSIESDLKKREQASKSKKSWLNRLNNWLFGWNDL

Entity 11 Entity 11 polymer

Description description	Nucleoporin ASM4
Molecular weight formula_weight	58853.9 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	V,X

MFGIRSGNNNGGFTNLTSQAPQTTQMFQSQSQLQPQPQPQPQQQQQHLQFNGSSDASSLRFGNSLSNTVNANNYSSNIGNNSINNNNIKNGTNNISQHGQGNNPSWVNNPKKRFTPHTVIRRKTTKQNSSSDINQNDDSSSMNATMRNFSKQNQDSKHNERNKSAANNDINSLLSNFNDIPPSVTLQDWQREDEFGSIPSLTTQFVTDKYTAKKTNRSAYDSKNTPNVFDKDSYVRIANIEQNHLDNNYNTAETNNKVHETSSKSSSLSAIIVFGYPESISNELIEHFSHFGHIMEDFQVLRLGRGINPNTFRIFHNHDTGCDENDSTVNKSITLKGRNNESNNKKYPIFTGESWVKLTYNSPSSALRALQENGTIFRGSLIGCIPYSKNAVEQLAGCKIDNVDDIGEFNVSMYQNSSTSSTSNTPSPPNVIITDGTLLREDDNTPAGHAGNPTNISSPIVANSPNKRLDVIDGKLPFMQNAGPNSNIPNLLRNLESKMRQQEAKYRNNEPAGFTHKLSNWLFGWNDL

Entity 12 Entity 12 polymer

Description description	Nucleoporin NUP82
Molecular weight formula_weight	82174.0 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	u,v

MSQSSRLSALPIFQASLSASQSPRYIFSSQNGTRIVFIQDNIIRWYNVLTDSLYHSLNFSRHLVLDDTFHVISSTSGDLLCLFNDNEIFVMEVPWGYSNVEDVSIQDAFQIFHYSIDEEEVGPKSSIKKVLFHPKSYRDSCIVVLKEDDTITMFDILNSQEKPIVLNKPNNSFGLDARVNDITDLEFSKDGLTLYCLNTTEGGDIFAFYPFLPSVLLLNEKDLNLILNKSLVMYESLDSTTDVIVKRNVIKQLQFVSKLHENWNSRFGKVDIQKEYRLAKVQGPFTINPFPGELYDYTATNIATILIDNGQNEIVCVSFDDGSLILLFKDLEMSMSWDVDNYVYNNSLVLIERVKLQREIKSLITLPEQLGKLYVISDNIIQQVNFMSWASTLSKCINESDLNPLAGLKFESKLEDIATIERIPNLAYINWNDQSNLALMSNKTLTFQNISSDMKPQSTAAETSISTEKSDTVGDGFKMSFTQPINEILILNDNFQKACISPCERIIPSADRQIPLKNEASENQLEIFTDISKEFLQRIVKAQTLGVSIHNRIHEQQFELTRQLQSTCKIISKDDDLRRKFEAQNKKWDAQLSRQSELMERFSKLSKKLSQIAESNKFKEKKISHGEMKWFKEIRNQILQFNSFVHSQKSLQQDLSYLKSELTRIEAETIKVDKKSQNEWDELRKMLEIDSKIIKECNEELLQVSQEFTTKTQ

Entity 13 Entity 13 polymer

Description description	Nucleoporin NUP159
Molecular weight formula_weight	159067.2 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	w,x

MSSLKDEVPTETSEDFGFKFLGQKQILPSFNEKLPFASLQNLDISNSKSLFVAASGSKAVVGELQLLRDHITSDSTPLTFKWEKEIPDVIFVCFHGDQVLVSTRNALYSLDLEELSEFRTVTSFEKPVFQLKNVNNTLVILNSVNDLSALDLRTKSTKQLAQNVTSFDVTNSQLAVLLKDRSFQSFAWRNGEMEKQFEFSLPSELEELPVEEYSPLSVTILSPQDFLAVFGNVISETDDEVSYDQKMYIIKHIDGSASFQETFDITPPFGQIVRFPYMYKVTLSGLIEPDANVNVLASSCSSEVSIWDSKQVIEPSQDSERAVLPISEETDKDTNPIGVAVDVVTSGTILEPCSGVDTIERLPLVYILNNEGSLQIVGLFHVAAIKSGHYSINLESLEHEKSLSPTSEKIPIAGQEQEEKKKNNESSKALSENPFTSANTSGFTFLKTQPAAANSLQSQSSSTFGAPSFGSSAFKIDLPSVSSTSTGVASSEQDATDPASAKPVFGKPAFGAIAKEPSTSEYAFGKPSFGAPSFGSGKSSVESPASGSAFGKPSFGTPSFGSGNSSVEPPASGSAFGKPSFGTPSFGSGNSSAEPPASGSAFGKPSFGTSAFGTASSNETNSGSIFGKAAFGSSSFAPANNELFGSNFTISKPTVDSPKEVDSTSPFPSSGDQSEDESKSDVDSSSTPFGTKPNTSTKPKTNAFDFGSSSFGSGFSKALESVGSDTTFKFGTQASPFSSQLGNKSPFSSFTKDDTENGSLSKGSTSEINDDNEEHESNGPNVSGNDLTDSTVEQTSSTRLPETPSDEDGEVVEEEAQKSPIGKLTETIKKSANIDMAGLKNPVFGNHVKAKSESPFSAFATNITKPSSTTPAFSFGNSTMNKSNTSTVSPMEEADTKETSEKGPITLKSVENPFLPAKEERTGESSKKDHNDDPKDGYVSGSEISVRTSESAFDTTANEEIPKSQDVNNHEKSETDPKYSQHAVVDHDNKSKEMNETSKNNERSGQPNHGVQGDGIALKKDNEKENFDSNMAIKQFEDHQSSEEDASEKDSRQSSEVKESDDNMSLNSDRDESISESYDKLEDINTDELPHGGEAFKAREVSASADFDVQTSLEDNYAESGIQTDLSESSKENEVQTDAIPVKHNSTQTVKKEAVDNGLQTEPVETCNFSVQTFEGDENYLAEQCKPKQLKEYYTSAKVSNIPFVSQNSTLRLIESTFQTVEAEFTVLMENIRNMDTFFTDQSSIPLVKRTVRSINNLYTWRIPEAEILLNIQNNIKCEQMQITNANIQDLKEKVTDYVRKDIAQITEDVANAKEEYLFLMHFDDASSGYVKDLSTHQFRMQKTLRQKLFDVSAKINHTEELLNILKLFTVKNKRLDDNPLVAKLAKESLARDGLLKEIKLLREQVSRLQLEEKGKKASSFDASSSITKDMKGFKVVEVGLAMNTKKQIGDFFKNLNMAK

Entity 14 Entity 14 polymer

Description description	Nucleoporin NUP120
Molecular weight formula_weight	120560.3 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	h,a

MACLSRIDANLLQYYEKPEPNNTVDLYVSNNSNNNGLKEGDKSISTPVPQPYGSEYSNCLLLSNSEYICYHFSSRSTLLTFYPLSDAYHGKTINIHLPNASMNQRYTLTIQEVEQQLLVNVILKDGSFLTLQLPLSFLFSSANTLNGEWFHLQNPYDFTVRVPHFLFYVSPQFSVVFLEDGGLLGLKKVDGVHYEPLLFNDNSYLKSLTRFFSRSSKSDYDSVISCKLFHERYLIVLTQNCHLKIWDLTSFTLIQDYDMVSQSDSDPSHFRKVEAVGEYLSLYNNTLVTLLPLENGLFQMGTLLVDSSGILTYTFQNNIPTNLSASAIWSIVDLVLTRPLELNVEASYLNLIVLWKSGTASKLQILNVNDESFKNYEWIESVNKSLVDLQSEHDLDIVTKTGDVERGFCNLKSRYGTQIFERAQQILSENKIIMAHNEDEEYLANLETILRDVKTAFNEASSITLYGDEIILVNCFQPYNHSLYKLNTTVENWFYNMHSETDGSELFKYLRTLNGFASTLSNDVLRSISKKFLDIITGELPDSMTTVEKFTDIFKNCLENQFEITNLKILFDELNSFDIPVVLNDLINNQMKPGIFWKKDFISAIKFDGFTSIISLESLHQLLSIHYRITLQVLLTFVLFDLDTEIFGQHISTLLDLHYKQFLLLNLYRQDKCLLAEVLLKDSSEFSFGVKFFNYGQLIAYIDSLNSNVYNASITENSFFMTFFRSYIIENTSHKNIRFFLENVECPFYLRHNEVQEFMFAMTLFSCGNFDQSYEIFQLHDYPEAINDKLPTFLEDLKSENYHGDSIWKDLLCTFTVPYRHSAFYYQLSLLFDRNNSQEFALKCISKSAEYSLKEIQIEELQDFKEKQHIHYLNLLIHFRMFEEVLDVLRLGHECLSDTVRTNFLQLLLQEDIYSRDFFSTLLRLCNAHSDNGELYLRTVDIKIVDSILSQNLRSGDWECFKKLYCFRMLNKSERAAAEVLYQYILMQADLDVIRKRKCYLMVINVLSSFDSAYDQWILNGSKVVTLTDLRDELRGL

Entity 15 Entity 15 polymer

Description description	Nucleoporin NUP85
Molecular weight formula_weight	84972.4 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	i,b

MTIDDSNRLLMDVDQFDFLDDGTAQLSNNKTDEEEQLYKRDPVSGAILVPMTVNDQPIEKNGDKMPLKFKLGPLSYQNMAFITAKDKYKLYPVRIPRLDTSKEFSAYVSGLFEIYRDLGDDRVFNVPTIGVVNSNFAKEHNATVNLAMEAILNELEVFIGRVKDQDGRVNRFYELEESLTVLNCLRTMYFILDGQDVEENRSEFIESLLNWINRSDGEPDEEYIEQVFSVKDSTAGKKVFETQYFWKLLNQLVLRGLLSQAIGCIERSDLLPYLSDTCAVSFDAVSDSIELLKQYPKDSSSTFREWKNLVLKLSQAFGSSATDISGELRDYIEDFLLVIGGNQRKILQYSRTWYESFCGFLLYYIPSLELSAEYLQMSLEANVVDITNDWEQPCVDIISGKIHSILPVMESLDSCTAAFTAMICEAKGLIENIFEGEKNSDDYSNEDNEMLEDLFSYRNGMASYMLNSFAFELCSLGDKELWPVAIGLIALSATGTRSAKKMVIAELLPHYPFVTNDDIEWMLSICVEWRLPEIAKEIYTTLGNQMLSAHNIIESIANFSRAGKYELVKSYSWLLFEASCMEGQKLDDPVLNAIVSKNSPAEDDVIIPQDILDCVVTNSMRQTLAPYAVLSQFYELRDREDWGQALRLLLLLIEFPYLPKHYLVLLVAKFLYPIFLLDDKKLMDEDSVATVIEVIETKWDDADEKSSNLYETIIEADKSLPSSMATLLKNLRKKLNFKLCQAFM

Entity 16 Entity 16 polymer

Description description	Nucleoporin 145c
Molecular weight formula_weight	81157.9 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	j,c

SIWGLVNEEDAEIDEDDLSKQEDGGEQPLRKVRTLAQSKPSDKEVILKTDGTFGTLSGKDDSIVEEKAYEPDLSDADFEGIEASPKLDVSKDWVEQLILAGSSLRSVFATSKEFDGPCQNEIDLLFSECNDEIDNAKLIMKERRFTASYTFAKFSTGSMLLTKDIVGKSGVSIKRLPTELQRKFLFDDVYLDKEIEKVTIEARKSNPYPQISESSLLFKDALDYMEKTSSDYNLWKLSSILFDPVSYPYKTDNDQVKMALLKKERHCRLTSWIVSQIGPEIEEKIRNSSNEIEQIFLYLLLNDVVRASKLAIESKNGHLSVLISYLGSNDPRIRDLAELQLQKWSTGGCSIDKNISKIYKLLSGSPFEGLFSLKELESEFSWLCLLNLTLCYGQIDEYSLESLVQSHLDKFSLPYDDPIGVIFQLYAANENTEKLYKEVRQRTNALDVQFCWYLIQTLRFNGTRVFSKETSDEATFAFAAQLEFAQLHGHSLFVSCFLNDDKAAEDTIKRLVMREITLLRASTNDHILNRLKIPSQLIFNAQALKDRYEGNYLSEVQNLLLGSSYDLAEMAIVTSLGPRLLLSNNPVQNNELKTLREILNEFPDSERDKWSVSINVFEVYLKLVLDNVETQETIDSLISGMKIFYDQYKHCREVAACCNVMSQEIVSKILEKNNPSIGDSKAKLLELPLGQPEKAYLRGEFAQDLMKCTYKI

Entity 17 Entity 17 polymer

Description description	Protein transport protein SEC13
Molecular weight formula_weight	33083.0 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	k,d

MVVIANAHNELIHDAVLDYYGKRLATCSSDKTIKIFEVEGETHKLIDTLTGHEGPVWRVDWAHPKFGTILASCSYDGKVLIWKEENGRWSQIAVHAVHSASVNSVQWAPHEYGPLLLVASSDGKVSVVEFKENGTTSPIIIDAHAIGVNSASWAPATIEEDGEHNGTKESRKFVTGGADNLVKIWKYNSDAQTYVLESTLEGHSDWVRDVAWSPTVLLRSYLASVSQDRTCIIWTQDNEQGPWKKTLLKEEKFPDVLWRASWSLSGNVLALSGGDNKVTLWKENLEGKWEPAGEVHQ

Entity 18 Entity 18 polymer

Description description	Nucleoporin SEH1
Molecular weight formula_weight	39170.8 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	l,e

MQPFDSGHDDLVHDVVYDFYGRHVATCSSDQHIKVFKLDKDTSNWELSDSWRAHDSSIVAIDWASPEYGRIIASASYDKTVKLWEEDPDQEECSGRRWNKLCTLNDSKGSLYSVKFAPAHLGLKLACLGNDGILRLYDALEPSDLRSWTLTSEMKVLSIPPANHLQSDFCLSWCPSRFSPEKLAVSALEQAIIYQRGKDGKLHVAAKLPGHKSLIRSISWAPSIGRWYQLIATGCKDGRIRIFKITEKLSPLASEESLTNSNMFDNSADVDMDAQGRSDSNTEEKAELQSNLQVELLSEHDDHNGEVWSVSWNLTGTILSSAGDDGKVRLWKATYSNEFKCMSVITAQQ

Entity 19 Entity 19 polymer

Description description	Nucleoporin NUP84
Molecular weight formula_weight	83718.9 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	m,f

MELSPTYQTERFTKFSDTLKEFKIEQNNEQNPIDPFNIIREFRSAAGQLALDLANSGDESNVISSKDWELEARFWHLVELLLVFRNADLDLDEMELHPYNSRGLFEKKLMQDNKQLYQIWIVMVWLKENTYVMERPKNVPTSKWLNSITSGGLKSCDLDFPLRENTNVLDVKDKEEDHIFFKYIYELILAGAIDEALEEAKLSDNISICMILCGIQEYLNPVIDTQIANEFNTQQGIKKHSLWRRTVYSLSQQAGLDPYERAIYSYLSGAIPNQEVLQYSDWESDLHIHLNQILQTEIENYLLENNQVGTDELILPLPSHALTVQEVLNRVASRHPSESEHPIRVLMASVILDSLPSVIHSSVEMLLDVVKGTEASNDIIDKPYLLRIVTHLAICLDIINPGSVEEVDKSKLITTYISLLKLQGLYENIPIYATFLNESDCLEACSFILSSLEDPQVRKKQIETINFLRLPASNILRRTTQRVFDETEQEYSPSNEISISFDVNNIDMHLIYGVEWLIEGKLYVDAVHSIIALSRRFLLNGRVKALEQFMERNNIGEICKNYELEKIADNISKDENEDQFLEEITQYEHLIKGIREYEEWQKSVSLLSSESNIPTLIEKLQGFSKDTFELIKTFLVDLTSSNFADSADYEILYEIRALYTPFLLMELHKKLVEAAKLLKIPKFISEALAFTSLVANENDKIYLLFQSSGKLKEYLDLVARTATLSN

Entity 20 Entity 20 polymer

Description description	Nucleoporin NUP133
Molecular weight formula_weight	133452.7 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	n,g

MSEKKVHLRLRKELSVPIAVVENESLAQLSYEEESQASLMDISMEQQQLRLHSHFDNSKVFTENNRYIVKTLQTDYSSGFSNDDELNGYIDMQIGYGLVNDHKKVYIWNIHSTQKDTPYITVPFRSDDNDEIAVAPRCILTFPATMDESPLALNPNDQDETGGLIIIKGSKAIYYEDINSINNLNFKLSEKFSHELELPINSSGGEKCDLMLNCEPAGIVLSTNMGRIFFITIRNSMGKPQLKLGKLLNKPFKLGIWSKIFNTNSSVVSLRNGPILGKGTRLVYITTNKGIFQTWQLSATNSHPTKLIDVNIYEAILESLQDLYPFAHGTLKIWDSHPLQDESSQLFLSSIYDSSCNETYYILSTIIFDSSSNSFTIFSTYRLNTFMESITDTKFKPKIFIPQMENANDTNEVTSILVMFPNAVVITQVNSKLDSSYSMRRKWEDIVSLRNDIDIIGSGYDSKSLYVLTKQMGVLQFFVKENEETNSKPEVGFVKSHVDQAVYFSKINANPIDFNLPPEISLDQESIEHDLKLTSEEIFHSNGKYIPPMLNTLGQHLSVRKEFFQNFLTFVAKNFNYKISPELKLDLIEKFEILNCCIKFNSIIRQSDVLNDIWEKTLSNYNLTQNEHLTTKTVVINSPDVFPVIFKQFLNHVVFVLFPSQNQNFKLNVTNLINLCFYDGILEEGEKTIRYELLELDPMEVDTSKLPWFINFDYLNCINQCFFDFTFACEEEGSLDSYKEGLLKIVKILYYQFNQFKIWINTQPVKSVNANDNFININNLYDDNHLDWNHVLCKVNLKEQCIQIAEFYKDLSGLVQTLQTLDQNDSTTVSLYETFFNEFPKEFSFTLFEYLIKHKKLNDLIFRFPQQHDVLIQFFQESAPKYGHVAWIQQILDGSYADAMNTLKNITVDDSKKGESLSECELHLNVAKLSSLLVEKDNLDINTLRKIQYNLDTIDAEKNISNKLKKGEVQICKRFKNGSIREVFNILVEELKSTTVVNLSDLVELYSMLDDEESLFIPLRLLSVDGNLLNFEVKKFLNALVWRRIVLLNASNEGDKLLQHIVKRVFDEELPKNNDFPLPSVDLLCDKSLLTPEYISETYGRFPIDQNAIREEIYEEISQVETLNSDNSLEIKLHSTIGSVAKEKNYTINYETNTVEY

Entity 21 Entity 21 polymer

Description description	Dynein light chain 1, cytoplasmic
Molecular weight formula_weight	10457.0 kDa
Source method src_method	nat
Copy count entity_count	6

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	o,p,q,r,s,t

MSDENKSTPIVKASDITDKLKEDILTISKDALDKYQLERDIAGTVKKQLDVKYGNTWHVIVGKNFGSYVTHEKGHFVYFYIGPLAFLVFKTA

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8. Citations (1)

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	37.00 Å
Resolution测定方法 resolution_method	—
颗粒数 num_particles	518

fit	/app/data/saxs_computation/n9/7n9f/7n9f.fit
pddf	/app/data/pr_computation/n9/7n9f/7n9f.pr.out
profile	/app/data/saxs_computation/n9/7n9f/7n9f.dat

Structure of the in situ yeast NPC

1. Protein Identity and Related Structures Protein Identity & Related Structures

Nucleoporin NUP170

State in the Current Structure

Other States of the Same Protein in the Database

Nucleoporin NUP157

State in the Current Structure

Other States of the Same Protein in the Database

Nucleoporin NSP1

State in the Current Structure

Other States of the Same Protein in the Database

Nucleoporin NUP57

State in the Current Structure

Other States of the Same Protein in the Database

Nucleoporin NUP49/NSP49

State in the Current Structure

Other States of the Same Protein in the Database

Nucleoporin NUP192

State in the Current Structure

Other States of the Same Protein in the Database

Nucleoporin NUP188

State in the Current Structure

Other States of the Same Protein in the Database

Nucleoporin NIC96

State in the Current Structure

Other States of the Same Protein in the Database

Nucleoporin NUP53

State in the Current Structure

Other States of the Same Protein in the Database

Nucleoporin ASM4

State in the Current Structure

Other States of the Same Protein in the Database

Nucleoporin NUP82

State in the Current Structure

Other States of the Same Protein in the Database

Nucleoporin NUP159

State in the Current Structure

Other States of the Same Protein in the Database

Nucleoporin NUP120

State in the Current Structure

Other States of the Same Protein in the Database

Nucleoporin NUP85

State in the Current Structure

Other States of the Same Protein in the Database

Nucleoporin 145c

State in the Current Structure

Other States of the Same Protein in the Database

Protein transport protein SEC13

State in the Current Structure

Other States of the Same Protein in the Database

Nucleoporin SEH1

State in the Current Structure

Other States of the Same Protein in the Database

Nucleoporin NUP84

State in the Current Structure

Other States of the Same Protein in the Database

Nucleoporin NUP133

State in the Current Structure

Other States of the Same Protein in the Database

Dynein light chain 1, cytoplasmic

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

6. Entities and Polymers Entities & Polymers (21)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Entity 4 Entity 4 polymer

Polymer Polymer