8h7g

Cryo-EM structure of the human SAGA complex

Method: ELECTRON MICROSCOPY
▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

Transformation/transcription domain-associated protein

OrganismNot specified

UniProt Q9Y4A5

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 14 Splicing factor 3B subunit 3 × 1 (Q15393) Splicing factor 3B subunit 5 × 1 (Q9BWJ5) Transcription factor SPT20 homolog × 1 (Q8NEM7) Transcription initiation protein SPT3 homolog × 1 (O75486) Transcriptional adapter 1 × 1 (Q96BN2) TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L × 1 (O75529) STAGA complex 65 subunit gamma × 1 (O94864) TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L × 1 (Q9Y6J9) Transcription initiation factor TFIID subunit 9 × 1 (Q16594) Transcription initiation factor TFIID subunit 10 × 1 (Q12962) Transcription initiation factor TFIID subunit 12 × 1 (Q16514) Unassigned sequence × 1 Ataxin-7 × 1 (O15265) Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name TRRAP_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain C; PDBConstruct 1–3859; UniProt 1–3859

Splicing factor 3B subunit 3

OrganismNot specified

UniProt Q15393

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 14 Transformation/transcription domain-associated protein × 1 (Q9Y4A5) Splicing factor 3B subunit 5 × 1 (Q9BWJ5) Transcription factor SPT20 homolog × 1 (Q8NEM7) Transcription initiation protein SPT3 homolog × 1 (O75486) Transcriptional adapter 1 × 1 (Q96BN2) TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L × 1 (O75529) STAGA complex 65 subunit gamma × 1 (O94864) TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L × 1 (Q9Y6J9) Transcription initiation factor TFIID subunit 9 × 1 (Q16594) Transcription initiation factor TFIID subunit 10 × 1 (Q12962) Transcription initiation factor TFIID subunit 12 × 1 (Q16514) Unassigned sequence × 1 Ataxin-7 × 1 (O15265) Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name SF3B3_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain A; PDBConstruct 1–1217; UniProt 1–1217

Splicing factor 3B subunit 5

OrganismNot specified

UniProt Q9BWJ5

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 14 Transformation/transcription domain-associated protein × 1 (Q9Y4A5) Splicing factor 3B subunit 3 × 1 (Q15393) Transcription factor SPT20 homolog × 1 (Q8NEM7) Transcription initiation protein SPT3 homolog × 1 (O75486) Transcriptional adapter 1 × 1 (Q96BN2) TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L × 1 (O75529) STAGA complex 65 subunit gamma × 1 (O94864) TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L × 1 (Q9Y6J9) Transcription initiation factor TFIID subunit 9 × 1 (Q16594) Transcription initiation factor TFIID subunit 10 × 1 (Q12962) Transcription initiation factor TFIID subunit 12 × 1 (Q16514) Unassigned sequence × 1 Ataxin-7 × 1 (O15265) Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name SF3B5_HUMAN
Isoform —
PDB entities 3
Chains and sequence ranges Author chain B; PDBConstruct 1–86; UniProt 1–86

Transcription factor SPT20 homolog

OrganismNot specified

UniProt Q8NEM7

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 14 Transformation/transcription domain-associated protein × 1 (Q9Y4A5) Splicing factor 3B subunit 3 × 1 (Q15393) Splicing factor 3B subunit 5 × 1 (Q9BWJ5) Transcription initiation protein SPT3 homolog × 1 (O75486) Transcriptional adapter 1 × 1 (Q96BN2) TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L × 1 (O75529) STAGA complex 65 subunit gamma × 1 (O94864) TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L × 1 (Q9Y6J9) Transcription initiation factor TFIID subunit 9 × 1 (Q16594) Transcription initiation factor TFIID subunit 10 × 1 (Q12962) Transcription initiation factor TFIID subunit 12 × 1 (Q16514) Unassigned sequence × 1 Ataxin-7 × 1 (O15265) Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name SP20H_HUMAN
Isoform —
PDB entities 4
Chains and sequence ranges Author chain D; PDBConstruct 1–779; UniProt 1–779

Transcription initiation protein SPT3 homolog

OrganismNot specified

UniProt O75486

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 14 Transformation/transcription domain-associated protein × 1 (Q9Y4A5) Splicing factor 3B subunit 3 × 1 (Q15393) Splicing factor 3B subunit 5 × 1 (Q9BWJ5) Transcription factor SPT20 homolog × 1 (Q8NEM7) Transcriptional adapter 1 × 1 (Q96BN2) TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L × 1 (O75529) STAGA complex 65 subunit gamma × 1 (O94864) TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L × 1 (Q9Y6J9) Transcription initiation factor TFIID subunit 9 × 1 (Q16594) Transcription initiation factor TFIID subunit 10 × 1 (Q12962) Transcription initiation factor TFIID subunit 12 × 1 (Q16514) Unassigned sequence × 1 Ataxin-7 × 1 (O15265) Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name SUPT3_HUMAN
Isoform —
PDB entities 5
Chains and sequence ranges Author chain E; PDBConstruct 1–317; UniProt 1–317

Transcriptional adapter 1

Homo sapiens

UniProt Q96BN2

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 14 Transformation/transcription domain-associated protein × 1 (Q9Y4A5) Splicing factor 3B subunit 3 × 1 (Q15393) Splicing factor 3B subunit 5 × 1 (Q9BWJ5) Transcription factor SPT20 homolog × 1 (Q8NEM7) Transcription initiation protein SPT3 homolog × 1 (O75486) TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L × 1 (O75529) STAGA complex 65 subunit gamma × 1 (O94864) TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L × 1 (Q9Y6J9) Transcription initiation factor TFIID subunit 9 × 1 (Q16594) Transcription initiation factor TFIID subunit 10 × 1 (Q12962) Transcription initiation factor TFIID subunit 12 × 1 (Q16514) Unassigned sequence × 1 Ataxin-7 × 1 (O15265) Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name TADA1_HUMAN
Isoform —
PDB entities 6
Chains and sequence ranges Author chain G; PDBConstruct 40–374; UniProt 1–335

TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L

OrganismNot specified

UniProt O75529

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 14 Transformation/transcription domain-associated protein × 1 (Q9Y4A5) Splicing factor 3B subunit 3 × 1 (Q15393) Splicing factor 3B subunit 5 × 1 (Q9BWJ5) Transcription factor SPT20 homolog × 1 (Q8NEM7) Transcription initiation protein SPT3 homolog × 1 (O75486) Transcriptional adapter 1 × 1 (Q96BN2) STAGA complex 65 subunit gamma × 1 (O94864) TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L × 1 (Q9Y6J9) Transcription initiation factor TFIID subunit 9 × 1 (Q16594) Transcription initiation factor TFIID subunit 10 × 1 (Q12962) Transcription initiation factor TFIID subunit 12 × 1 (Q16514) Unassigned sequence × 1 Ataxin-7 × 1 (O15265) Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name TAF5L_HUMAN
Isoform —
PDB entities 7
Chains and sequence ranges Author chain H; PDBConstruct 1–589; UniProt 1–589

STAGA complex 65 subunit gamma

Homo sapiens

UniProt O94864

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 14 Transformation/transcription domain-associated protein × 1 (Q9Y4A5) Splicing factor 3B subunit 3 × 1 (Q15393) Splicing factor 3B subunit 5 × 1 (Q9BWJ5) Transcription factor SPT20 homolog × 1 (Q8NEM7) Transcription initiation protein SPT3 homolog × 1 (O75486) Transcriptional adapter 1 × 1 (Q96BN2) TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L × 1 (O75529) TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L × 1 (Q9Y6J9) Transcription initiation factor TFIID subunit 9 × 1 (Q16594) Transcription initiation factor TFIID subunit 10 × 1 (Q12962) Transcription initiation factor TFIID subunit 12 × 1 (Q16514) Unassigned sequence × 1 Ataxin-7 × 1 (O15265) Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name ST65G_HUMAN
Isoform —
PDB entities 8
Chains and sequence ranges Author chain I; PDBConstruct 1–414; UniProt 1–414

TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L

OrganismNot specified

UniProt Q9Y6J9

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 14 Transformation/transcription domain-associated protein × 1 (Q9Y4A5) Splicing factor 3B subunit 3 × 1 (Q15393) Splicing factor 3B subunit 5 × 1 (Q9BWJ5) Transcription factor SPT20 homolog × 1 (Q8NEM7) Transcription initiation protein SPT3 homolog × 1 (O75486) Transcriptional adapter 1 × 1 (Q96BN2) TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L × 1 (O75529) STAGA complex 65 subunit gamma × 1 (O94864) Transcription initiation factor TFIID subunit 9 × 1 (Q16594) Transcription initiation factor TFIID subunit 10 × 1 (Q12962) Transcription initiation factor TFIID subunit 12 × 1 (Q16514) Unassigned sequence × 1 Ataxin-7 × 1 (O15265) Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name TAF6L_HUMAN
Isoform —
PDB entities 9
Chains and sequence ranges Author chain K; PDBConstruct 1–622; UniProt 1–622

Transcription initiation factor TFIID subunit 9

OrganismNot specified

UniProt Q16594

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 14 Transformation/transcription domain-associated protein × 1 (Q9Y4A5) Splicing factor 3B subunit 3 × 1 (Q15393) Splicing factor 3B subunit 5 × 1 (Q9BWJ5) Transcription factor SPT20 homolog × 1 (Q8NEM7) Transcription initiation protein SPT3 homolog × 1 (O75486) Transcriptional adapter 1 × 1 (Q96BN2) TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L × 1 (O75529) STAGA complex 65 subunit gamma × 1 (O94864) TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L × 1 (Q9Y6J9) Transcription initiation factor TFIID subunit 10 × 1 (Q12962) Transcription initiation factor TFIID subunit 12 × 1 (Q16514) Unassigned sequence × 1 Ataxin-7 × 1 (O15265) Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name TAF9_HUMAN
Isoform —
PDB entities 10
Chains and sequence ranges Author chain M; PDBConstruct 1–264; UniProt 1–264

Transcription initiation factor TFIID subunit 10

OrganismNot specified

UniProt Q12962

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 14 Transformation/transcription domain-associated protein × 1 (Q9Y4A5) Splicing factor 3B subunit 3 × 1 (Q15393) Splicing factor 3B subunit 5 × 1 (Q9BWJ5) Transcription factor SPT20 homolog × 1 (Q8NEM7) Transcription initiation protein SPT3 homolog × 1 (O75486) Transcriptional adapter 1 × 1 (Q96BN2) TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L × 1 (O75529) STAGA complex 65 subunit gamma × 1 (O94864) TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L × 1 (Q9Y6J9) Transcription initiation factor TFIID subunit 9 × 1 (Q16594) Transcription initiation factor TFIID subunit 12 × 1 (Q16514) Unassigned sequence × 1 Ataxin-7 × 1 (O15265) Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name TAF10_HUMAN
Isoform —
PDB entities 11
Chains and sequence ranges Author chain O; PDBConstruct 1–218; UniProt 1–218

Transcription initiation factor TFIID subunit 12

OrganismNot specified

UniProt Q16514

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 14 Transformation/transcription domain-associated protein × 1 (Q9Y4A5) Splicing factor 3B subunit 3 × 1 (Q15393) Splicing factor 3B subunit 5 × 1 (Q9BWJ5) Transcription factor SPT20 homolog × 1 (Q8NEM7) Transcription initiation protein SPT3 homolog × 1 (O75486) Transcriptional adapter 1 × 1 (Q96BN2) TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L × 1 (O75529) STAGA complex 65 subunit gamma × 1 (O94864) TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L × 1 (Q9Y6J9) Transcription initiation factor TFIID subunit 9 × 1 (Q16594) Transcription initiation factor TFIID subunit 10 × 1 (Q12962) Unassigned sequence × 1 Ataxin-7 × 1 (O15265) Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name TAF12_HUMAN
Isoform —
PDB entities 12
Chains and sequence ranges Author chain R; PDBConstruct 1–161; UniProt 1–161

Ataxin-7

OrganismNot specified

UniProt O15265

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 14 Transformation/transcription domain-associated protein × 1 (Q9Y4A5) Splicing factor 3B subunit 3 × 1 (Q15393) Splicing factor 3B subunit 5 × 1 (Q9BWJ5) Transcription factor SPT20 homolog × 1 (Q8NEM7) Transcription initiation protein SPT3 homolog × 1 (O75486) Transcriptional adapter 1 × 1 (Q96BN2) TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L × 1 (O75529) STAGA complex 65 subunit gamma × 1 (O94864) TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L × 1 (Q9Y6J9) Transcription initiation factor TFIID subunit 9 × 1 (Q16594) Transcription initiation factor TFIID subunit 10 × 1 (Q12962) Transcription initiation factor TFIID subunit 12 × 1 (Q16514) Unassigned sequence × 1 Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name ATX7_HUMAN
Isoform —
PDB entities 14
Chains and sequence ranges Author chain L; PDBConstruct 1–892; UniProt 1–892

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id8h7g
Deposition date deposition_date2022-10-20
Structure title titleCryo-EM structure of the human SAGA complex
Keywords keywordstranscription, epigenetics; TRANSCRIPTION
Experimental Method methodELECTRON MICROSCOPY
▼

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8h7g__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8h7g__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

8h7g__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg(Guinier)76.61 Å
Rg (electron density)77.24 Å
Total Rg77.27 Å
Atom count52609
Residues6593
Excluded volume943100 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 8h7g__assembly_1__model_1 tetradecameric (14) Success 4.1.3-1-20251215 (887e7ef) View Download
▶

4. Crystallography and Experiment 4. Crystallography & Experiment

▶

5. Entities and Polymers Entities & Polymers (14)

▼

6. Fold Classification (SCOP + CATH) 4 domains

CATH v4.4 (4 domains)

Domain ID domain_id8h7gA01
Class class2 — Mainly Beta
Architecture architecture130 — 7 Propeller
Topology topology10 — Methylamine Dehydrogenase; Chain H
Homologous superfamily homologous superfamily10 — YVTN repeat-like/Quinoprotein amine dehydrogenase
Domain ID domain_id8h7gA02
Class class2 — Mainly Beta
Architecture architecture130 — 7 Propeller
Topology topology10 — Methylamine Dehydrogenase; Chain H
Homologous superfamily homologous superfamily10 — YVTN repeat-like/Quinoprotein amine dehydrogenase
Domain ID domain_id8h7gH01
Class class1 — Mainly Alpha
Architecture architecture25 — Alpha Horseshoe
Topology topology40 — Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Homologous superfamily homologous superfamily500 — TFIID subunit TAF5, NTD2 domain
Domain ID domain_id8h7gK01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology20 — Histone, subunit A
Homologous superfamily homologous superfamily10 — Histone, subunit A
▶

7. Citations (1)