Alpha-synuclein
Homo sapiens
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein homooligomer Homooligomer Protein × 12 PDB declaration: 12-meric（12） Consistent with protein copy count | Chain A； UniProt 1–98 Chain B； UniProt 1–98 Chain C； UniProt 1–98 Chain D； UniProt 1–98 Chain E； UniProt 1–98 Chain F； UniProt 1–98 Chain G； UniProt 1–98 Chain H； UniProt 1–98 Chain I； UniProt 1–98 Chain J； UniProt 1–98 Chain K； UniProt 1–98 Chain L； UniProt 1–98 | Non-standard monomer：Yes (specific site not provided by mmCIF) | 1KI 2-bromanyl-4-[(~{E})-2-[6-[2-(2-fluoranylethoxy)ethyl-methyl-amino]-5-methyl-1,3-benzothiazol-2-yl]ethenyl]phenol × 15 | ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE | Resolution 2.90 Å |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 8ZMY | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 10XU Structure of amplified aSyn filament by using seed amplification assay (SAA) from MSA patient CSF. Deposited 2026-02-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
35–101（67 aa）
Chain B
35–101（67 aa）
Chain C
35–101（67 aa）
Chain D
35–101（67 aa）
Chain E
35–101（67 aa）
Chain U
35–101（67 aa）
Chain W
35–101（67 aa）
Chain X
35–101（67 aa）
Chain Y
35–101（67 aa）
Chain Z
35–101（67 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.18 Å |
| 1XQ8 Human micelle-bound alpha-synuclein Deposited 2004-10-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7.4；298.2 K；Ionic strength (raw mmCIF value) 115；Pressure 1
NMR sample composition
0.5 MM ALPHA-SYNUCLEIN 2H, 13C, 15N
|
Resolution not provided |
| 2JN5 Solution Structure of a Dodecapeptide from Alpha-Synuclein Bound with Synphilin-1 Deposited 2006-12-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–12（12 aa）
Fragment：residues 1-12
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6.5；298 K；Pressure ambient
NMR sample composition
1 mM entity, 20 mM sodium phosphate, 50 mM sodium chloride, 0.1 mM Synphilin-1, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2KKW SLAS-micelle bound alpha-synuclein Deposited 2009-06-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
Experimental method not declared
NMR measurement conditions
pH 7.4；298.2 K；Ionic strength (raw mmCIF value) 0.05；Pressure AMBIENT
NMR sample composition
0.75 MM [U-100% 13C, U-100% 15N, U-80% 2H] ALPHA-SYNUCLEIN, 75 MM SODIUM LAUROYL SARCOSINATE, 95% H2O/5% D2O
|
Resolution not provided |
| 2M55 NMR structure of the complex of an N-terminally acetylated alpha-synuclein peptide with calmodulin Deposited 2013-02-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–19（19 aa）
Fragment：UNP residues 1-19
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 4 |
SOLUTION NMR
NMR measurement conditions
pH 6.36；310 K；Pressure ambient
NMR sample composition
600 uM [U-99% 13C; U-99% 15N] calmodulin, 600 uM alpha-synuclein, 50 mM MES, 100 mM potassium chloride, 3 mM calcium ion, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
700 uM [U-13C; U-15N]-Met,Val,Phe,Gly,Leu,Ala alpha-synuclein, 700 uM calmodulin, 50 mM MES, 100 mM potassium chloride, 3 mM calcium ion, 0.1% sodium azide, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
150 uM [U-99% 13C; U-99% 15N] calmodulin, 150 uM alpha-synuclein, 50 mM MES, 100 mM potassium chloride, 3 mM calcium ion, 10 mg/mL Pf1 phage, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 2N0A Atomic-resolution structure of alpha-synuclein fibrils Deposited 2015-03-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
SOLID-STATE NMR
NMR measurement conditions
pH 7；Pressure ambient
NMR sample composition
66% [U-100% 13C; U-100% 15N] alpha-synuclein, 34% H2O, Rehydrated Fibril | Rehydrated Fibril
NMR sample composition
66% [U-100% 13C 1,3-Glycerol; U-100% 15N] alpha-synuclein, 34% H2O, Rehydrated Fibril | Rehydrated Fibril
NMR sample composition
66% [U-100% 13C 2-Glycerol; U-100% 15N] alpha-synuclein, 34% H2O, Rehydrated Fibril | Rehydrated Fibril
NMR sample composition
66% [U-25% 13C; U-25% 15N] alpha-synuclein, 34% H2O, Rehydrated Fibril | Rehydrated Fibril
NMR sample composition
66% [U-25% 13C 1,3-Glycerol; natural abundance N][natural abundance C13, U-50% N15] alpha-synuclein, 34% H2O, Rehydrated Fibril | Rehydrated Fibril
NMR sample composition
66% [U-25% 13C 2-Glycerol; natural abundance N][natural abundance C13, U-50% N15] alpha-synuclein, 34% H2O, Rehydrated Fibril | Rehydrated Fibril
NMR sample composition
90% Adamantane, 10% KBr, Dry | Dry
|
Resolution not provided |
| 2X6M Structure of a single domain camelid antibody fragment in complex with a C-terminal peptide of alpha-synuclein Deposited 2010-02-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
132–140（9 aa）
Fragment：C-TERMINAL FRAGMENT OF ALPHA-SYNUCLEIN, RESIDUES 132-140
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；25% PEG6000, 100 MM HEPES PH 7.5, 100 MM LICL
|
Resolution 1.62 Å R-free 0.203 |
| 3Q25 Crystal structure of human alpha-synuclein (1-19) fused to maltose binding protein (MBP) Deposited 2010-12-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–19（19 aa）
|
Not recorded | SO4 SULFATE ION × 10 GOL GLYCEROL × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；290 K；2.2 M AMMONIUM SULFATE, 20% (w/v) GLYCEROL, pH 8.0, vapor diffusion, hanging drop, temperature 290K
|
Resolution 1.90 Å R-free 0.196 |
| 3Q25 Crystal structure of human alpha-synuclein (1-19) fused to maltose binding protein (MBP) Deposited 2010-12-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–19（19 aa）
|
Not recorded | SO4 SULFATE ION × 20 GOL GLYCEROL × 12 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；290 K；2.2 M AMMONIUM SULFATE, 20% (w/v) GLYCEROL, pH 8.0, vapor diffusion, hanging drop, temperature 290K
|
Resolution 1.90 Å R-free 0.196 |
| 3Q26 Cyrstal structure of human alpha-synuclein (10-42) fused to maltose binding protein (MBP) Deposited 2010-12-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
10–42（33 aa）
|
Not recorded | GOL GLYCEROL × 6 SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；290 K；0.2M tri-lithium citrate, 2.2 M ammonium sulfate, pH 8.0, vapor diffusion, hanging drop, temperature 290K
|
Resolution 1.54 Å R-free 0.183 |
| 3Q27 Cyrstal structure of human alpha-synuclein (32-57) fused to maltose binding protein (MBP) Deposited 2010-12-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
32–57（26 aa）
|
Not recorded | SO4 SULFATE ION × 3 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；290 K；0.1M bicine pH 9.0, 2.4 M ammonium sulfate, vapor diffusion, hanging drop, temperature 290K
|
Resolution 1.30 Å R-free 0.156 |
| 3Q28 Cyrstal structure of human alpha-synuclein (58-79) fused to maltose binding protein (MBP) Deposited 2010-12-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
58–79（22 aa）
|
Not recorded | SO4 SULFATE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；290 K；0.1M BICINE pH 9.0, 3.2 M AMMONIUM SULFATE, vapor diffusion, hanging drop, temperature 290K
|
Resolution 1.60 Å R-free 0.173 |
| 3Q29 Cyrstal structure of human alpha-synuclein (1-19) fused to maltose binding protein (MBP) Deposited 2010-12-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–19（19 aa）
Chain C
1–19（19 aa）
|
Not recorded | GOL GLYCEROL × 4 SO4 SULFATE ION × 11 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；290 K；0.1M TRIS pH 8.0, 2.4 M AMMONIUM SULFATE, vapor diffusion, hanging drop, temperature 290K
|
Resolution 2.30 Å R-free 0.232 |
| 3Q29 Cyrstal structure of human alpha-synuclein (1-19) fused to maltose binding protein (MBP) Deposited 2010-12-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–19（19 aa）
Chain C
1–19（19 aa）
|
Not recorded | GOL GLYCEROL × 4 SO4 SULFATE ION × 11 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；290 K；0.1M TRIS pH 8.0, 2.4 M AMMONIUM SULFATE, vapor diffusion, hanging drop, temperature 290K
|
Resolution 2.30 Å R-free 0.232 |
| 4BXL Structure of alpha-synuclein in complex with an engineered binding protein Deposited 2013-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
35–56（22 aa）
Fragment：RESIDUES 35-56
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7.4；303 K；Ionic strength (raw mmCIF value) 0.07；Pressure 1.0
NMR measurement conditions
pH 7.4；303 K；Ionic strength (raw mmCIF value) 0.07；Pressure 1.0
NMR sample composition
93% H2O/7% D2O
NMR sample composition
93% H2O/7% D2O
|
Resolution not provided |
| 4R0U Tgvtava, an amyloid forming segment from alpha synuclein, residues 72-78 Deposited 2014-08-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
72–78（7 aa）
Fragment：UNP RESIDUES 72-78
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2M magnesium formate, vapor diffusion, hanging drop, temperature 298K
|
Resolution 1.38 Å R-free 0.217 |
| 4R0W Vvtgvta, an amyloid forming segment from alpha synuclein, residues 70-76 Deposited 2014-08-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
70–76（7 aa）
Fragment：UNP RESIDUES 70-76
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；isopropanol, sodium cacodylate, pH 6.5, vapor diffusion, hanging drop, temperature 298K
|
Resolution 1.50 Å R-free 0.274 |
| 4RIK Amyloid forming segment, AVVTGVTAV, from the NAC domain of Parkinson's disease protein alpha-synuclein, residues 69-77 Deposited 2014-10-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
69–77（9 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；273 K；0.9M Ammonium Phosphate, 0.1M Sodium Acetate, pH 4.6, vapor diffusion, hanging drop, temperature 273K
|
Resolution 1.85 Å R-free 0.221 |
| 4RIL Structure of the amyloid forming segment, GAVVTGVTAVA, from the NAC domain of Parkinson's disease protein alpha-synuclein, residues 68-78, determined by electron diffraction Deposited 2014-10-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
68–78（11 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
cryo-EM vitrification conditions
Cryogen ETHANE
X-ray crystallization conditions
batch crystallization；pH 4；310 K；1 mg of synthetic peptide GAVVTGVTAVA was dissolved in 1 ml of sterile water and shaken overnight in an orbital mixing plate, pH 4.0, batch crystallization, temperature 310K
|
Resolution 1.43 Å R-free 0.275 |
| 4ZNN MicroED structure of the segment, GVVHGVTTVA, from the A53T familial mutant of Parkinson's disease protein, alpha-synuclein residues 47-56 Deposited 2015-05-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
47–56（10 aa）
|
Mutation：A53T | No recorded non-water small molecule |
ELECTRON CRYSTALLOGRAPHY
X-ray crystallization conditions
BATCH MODE；pH 7；310 K；1 mg of synthetic peptide GVVHGVTTVA was dissolved in 200 microliters of 50 mM phosphate buffer pH 7.0 and 0.1% w/v DMSO and shaken overnight in an orbital mixing plate
|
Resolution 1.41 Å R-free 0.282 |
| 6A6B cryo-em structure of alpha-synuclein fiber Deposited 2018-06-27 | Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain A
37–99（63 aa）
Fragment：UNP residues 37-99
Chain B
37–99（63 aa）
Fragment：UNP residues 37-99
Chain C
37–99（63 aa）
Fragment：UNP residues 37-99
Chain D
37–99（63 aa）
Fragment：UNP residues 37-99
Chain E
37–99（63 aa）
Fragment：UNP residues 37-99
Chain F
37–99（63 aa）
Fragment：UNP residues 37-99
Chain G
37–99（63 aa）
Fragment：UNP residues 37-99
Chain H
37–99（63 aa）
Fragment：UNP residues 37-99
Chain I
37–99（63 aa）
Fragment：UNP residues 37-99
Chain J
37–99（63 aa）
Fragment：UNP residues 37-99
Chain K
37–99（63 aa）
Fragment：UNP residues 37-99
Chain L
37–99（63 aa）
Fragment：UNP residues 37-99
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.07 Å |
| 6CU7 Alpha Synuclein fibril formed by full length protein - Rod Polymorph Deposited 2018-03-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 3
cryo-EM vitrification conditions
Cryogen ETHANE；blot force: 1
blot time: 4s
|
Resolution 3.50 Å |
| 6CU8 Alpha Synuclein fibril formed by full length protein - Twister Polymorph Deposited 2018-03-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 3
cryo-EM vitrification conditions
Cryogen ETHANE；blot force: 1
blot time: 4s
|
Resolution 3.60 Å |
| 6H6B Structure of alpha-synuclein fibrils Deposited 2018-07-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–121（121 aa）
Chain B
1–121（121 aa）
Chain C
1–121（121 aa）
Chain D
1–121（121 aa）
Chain E
1–121（121 aa）
Chain F
1–121（121 aa）
Chain G
1–121（121 aa）
Chain H
1–121（121 aa）
Chain I
1–121（121 aa）
Chain J
1–121（121 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.3
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 6I42 Structure of the alpha-Synuclein PreNAC/Cyclophilin A-complex Deposited 2018-11-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
48–60（13 aa）
Fragment：UNP residues 48-60
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7；293 K；1.93 M tri-ammonium citrate, pH 7.0
|
Resolution 1.38 Å R-free 0.178 |
| 6L1T Cryo-EM structure of phosphorylated Tyr39 a-synuclein amyloid fibril Deposited 2019-09-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.22 Å |
| 6L1U Cryo-EM structure of phosphorylated Tyr39 alpha-synuclein amyloid fibril Deposited 2019-09-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 15 PDB declaration: pentadecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
Chain O
1–140（140 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.37 Å |
| 6L4S cryo-em structure of alpha-synuclein fiber mutation type E46K Deposited 2019-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
45–99（55 aa）
Chain B
45–99（55 aa）
Chain C
45–99（55 aa）
Chain D
45–99（55 aa）
Chain E
45–99（55 aa）
Chain F
45–99（55 aa）
|
Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.37 Å |
| 6LRQ Cryo-EM structure of A53T alpha-synuclein amyloid fibril Deposited 2020-01-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Mutation：A53T Mutation：A53T Mutation：A53T Mutation：A53T Mutation：A53T Mutation：A53T | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.49 Å |
| 6OSJ Cryo-EM structure of the N-terminally acetylated full length alpha-synuclein fibrils (Ac1-140) Deposited 2019-05-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 6OSL Cryo-EM structure of the N-terminally acetylated C-terminal Alpha-synuclein truncation Ac1-122 Deposited 2019-05-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 6OSM Cryo-EM structure of the N-terminally acetylated C-terminal Alpha-synuclein truncation Ac1-103 Deposited 2019-05-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 6PEO Cryo-EM structure of alpha-synuclein H50Q Narrow Fibril Deposited 2019-06-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 6PES Cryo-EM structure of alpha-synuclein H50Q Wide Fibril Deposited 2019-06-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain V
1–140（140 aa）
Chain W
1–140（140 aa）
Chain X
1–140（140 aa）
Chain Y
1–140（140 aa）
Chain Z
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6RT0 cryo-em structure of alpha-synuclein fibril polymorph 2A Deposited 2019-05-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；3 uL aliquots were applied onto second glow-discharged 300 mesh copper Quantifoil grids R2 1
|
Resolution 3.10 Å |
| 6RTB cryo-em structure of alpha-synuclein fibril polymorph 2B Deposited 2019-05-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；3 uL aliquots were applied onto second glow-discharged 300 mesh copper Quantifoil grids R2 1
|
Resolution 3.46 Å |
| 6SST cryo-em structure of alpha-synuclein fibril polymorph 2B Deposited 2019-09-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；3 uL aliquots were applied onto second glow-discharged 300 mesh copper Quantifoil grids R2 1
|
Resolution 3.40 Å |
| 6SSX cryo-em structure of alpha-synuclein fibril polymorph 2A Deposited 2019-09-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；3 uL aliquots were applied onto second glow-discharged 300 mesh copper Quantifoil grids R2 1
|
Resolution 2.98 Å |
| 6UFR Structure of recombinantly assembled E46K alpha-synuclein fibrils Deposited 2019-09-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 6XYO Multiple system atrophy Type I alpha-synuclein filament Deposited 2020-01-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 6XYP Multiple system atrophy Type II-1 alpha-synuclein filament Deposited 2020-01-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.29 Å |
| 6XYQ Multiple system atrophy Type II-2 alpha-synuclein filament Deposited 2020-01-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.09 Å |
| 7C1D Cryo-EM structure of the hE46K cross-seeded hWT alpha-synuclein fibril Deposited 2020-05-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 7E0F CryoEM structure of G51D alpha-synuclein amyloid fibril Deposited 2021-01-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Mutation：G51D Mutation：G51D Mutation：G51D Mutation：G51D Mutation：G51D Mutation：G51D | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.02 Å |
| 7L7H Alpha-synuclein fibrils Deposited 2020-12-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.00 Å |
| 7LC9 Cryo-EM structure of the N-terminal alpha-synuclein truncation 41-140 Deposited 2021-01-10 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain A
41–140（100 aa）
Chain B
41–140（100 aa）
Chain C
41–140（100 aa）
Chain D
41–140（100 aa）
Chain E
41–140（100 aa）
Chain F
41–140（100 aa）
Chain G
41–140（100 aa）
Chain H
41–140（100 aa）
Chain I
41–140（100 aa）
Chain J
41–140（100 aa）
Chain K
41–140（100 aa）
Chain L
41–140（100 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 7NCA Type 1A alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 2 Deposited 2021-01-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE；LMB vitrobot IV
|
Resolution 3.47 Å |
| 7NCA Type 1A alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 2 Deposited 2021-01-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE；LMB vitrobot IV
|
Resolution 3.47 Å |
| 7NCG Type 2A alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 2 Deposited 2021-01-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE；LMB vitrobot IV
|
Resolution 3.43 Å |
| 7NCG Type 2A alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 2 Deposited 2021-01-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE；LMB vitrobot IV
|
Resolution 3.43 Å |
| 7NCH Type 1B alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 1 Deposited 2021-01-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE；LMB vitrobot IV
|
Resolution 3.84 Å |
| 7NCH Type 1B alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 1 Deposited 2021-01-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE；LMB vitrobot IV
|
Resolution 3.84 Å |
| 7NCI Type 2B alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 1 Deposited 2021-01-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE；LMB vitrobot IV
|
Resolution 3.55 Å |
| 7NCI Type 2B alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 1 Deposited 2021-01-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE；LMB vitrobot IV
|
Resolution 3.55 Å |
| 7NCJ Type 2AB alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 1 Deposited 2021-01-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE；LMB vitrobot IV
|
Resolution 4.23 Å |
| 7NCJ Type 2AB alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 1 Deposited 2021-01-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE；LMB vitrobot IV
|
Resolution 4.23 Å |
| 7NCK Type 3 alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 5 Deposited 2021-01-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE；LMB vitrobot IV
|
Resolution 3.18 Å |
| 7OZG PMCA-amplified alpha-synuclein fibril polymorph, Parkinson's Disease patient-derived seeds Deposited 2021-06-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 7OZH PMCA-amplified alpha-synuclein fibril polymorph, Multiple System Atrophy patient-derived seeds Deposited 2021-06-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.02 Å |
| 7STX Cryo-EM structure of human NatB in complex with CoA-Alpha-Synuclein Deposited 2021-11-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
1–5（5 aa）
Fragment：UNP residues 1-5
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | COA COENZYME A × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.14 Å |
| 7UAK Structure of recombinantly assembled A53E alpha-synuclein fibrils Deposited 2022-03-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Mutation：A53E Mutation：A53E Mutation：A53E Mutation：A53E Mutation：A53E Mutation：A53E | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.38 Å |
| 7V47 Type 1A alpha-synuclein fibril seeded by cerebrospinal fluid from a preclinical Parkinson's disease patient Deposited 2021-08-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 7V48 Type 1D alpha-synuclein fibril seeded by cerebrospinal fluid from a postmortal Parkinson's disease patient Deposited 2021-08-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 7V49 Type 4 alpha-synuclein fibril seeded by cerebrospinal fluid from a postmortal Parkinson's disease patient Deposited 2021-08-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 7V4A Heparin-induced alpha-synuclein fibrils polymorph 1 Deposited 2021-08-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 7V4B Heparin-induced alpha-synuclein fibrils polymorph 3 Deposited 2021-08-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 7V4C Heparin-induced alpha-synuclein fibrils polymorph 4 Deposited 2021-08-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 7V4D Heparin-remodelled alpha-synuclein fibrils Deposited 2021-08-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 7WMM alpha-synuclein fibril-F0502B complex Deposited 2022-01-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | 1KI 2-bromanyl-4-[(~{E})-2-[6-[2-(2-fluoranylethoxy)ethyl-methyl-amino]-5-methyl-1,3-benzothiazol-2-yl]ethenyl]phenol × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 7WNZ CryoEM structure of human alpha-synuclein A53T fibril Deposited 2022-01-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
36–100（65 aa）
Chain B
36–100（65 aa）
Chain C
36–100（65 aa）
Chain D
36–100（65 aa）
Chain E
36–100（65 aa）
Chain F
36–100（65 aa）
Chain G
36–100（65 aa）
Chain H
36–100（65 aa）
Chain I
36–100（65 aa）
Chain J
36–100（65 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 7WO0 CryoEM structure of human alpha-synuclein A53T fibril induced by calcium ions Deposited 2022-01-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 14 PDB declaration: tetradecameric |
Chain A
35–99（65 aa）
Chain B
35–99（65 aa）
Chain C
35–99（65 aa）
Chain D
35–99（65 aa）
Chain E
35–99（65 aa）
Chain F
35–99（65 aa）
Chain G
35–99（65 aa）
Chain O
35–99（65 aa）
Chain P
35–99（65 aa）
Chain Q
35–99（65 aa）
Chain R
35–99（65 aa）
Chain S
35–99（65 aa）
Chain T
35–99（65 aa）
Chain U
35–99（65 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 7XJX The cryo-EM structure of Fe3+ induced alpha-syn fibril. Deposited 2022-04-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 7XO0 Minor polymorph inalpha-synuclein fibril seeded by cerebrospinal fluid from a mid-to-late stage (mid-PD-1) Parkinson's disease patient Deposited 2022-04-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 7XO1 Major polymorph in alpha-synuclein fibril seeded by cerebrospinal fluid from a mid-to-late stage (mid-PD-1) Parkinson's disease patient Deposited 2022-04-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 7XO2 Minor polymorph in alpha-synuclein fibril seeded by cerebrospinal fluid from a mid-to-late stage (mid-PD-4) Parkinson's disease patient Deposited 2022-04-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain I
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 7XO3 Type 1C alpha-synuclein fibril seeded by cerebrospinal fluid from a mid-to-late stage (mid-PD-4) Parkinson's disease patient Deposited 2022-04-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 7YK2 Cryo-EM structure of Apo-alpha-syn fibril Deposited 2022-07-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 7YK8 Cryo-EM structure of dLAG3-alpha-syn fibril Deposited 2022-07-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 7YNF CCA-bound alpha-synuclein fibrils Deposited 2022-07-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | V79 copper;trisodium;18-(2-carboxylatoethyl)-20-(carboxylatomethyl)-12-ethenyl-7-ethyl-3,8,13,17-tetramethyl-17,18-dihydroporphyrin-21,23-diide-2-carboxylate × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 7YNG CR-bound alpha-synuclein fibrils Deposited 2022-07-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 7YNL EB-bound alpha-synuclein fibrils Deposited 2022-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
|
Not recorded | IZ8 4-azanyl-6-[[4-[4-[(~{E})-(8-azanyl-1-oxidanyl-5,7-disulfo-naphthalen-2-yl)diazenyl]-3-methyl-phenyl]-2-methyl-phenyl]diazenyl]-5-oxidanyl-naphthalene-1,3-disulfonic acid × 16 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 7YNM ThT-bound alpha-synuclein fibrils conformation 1 Deposited 2022-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | TFX 2-[4-(dimethylamino)phenyl]-3,6-dimethyl-1,3-benzothiazol-3-ium × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7YNN ThT-bound alpha-synuclein fibrils conformation 2 Deposited 2022-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | TFX 2-[4-(dimethylamino)phenyl]-3,6-dimethyl-1,3-benzothiazol-3-ium × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7YNO PiB-bound alpha-synuclein fibrils conformation 1 Deposited 2022-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | IZV 2-[4-(methylamino)phenyl]-1,3-benzothiazol-6-ol × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 7YNP BF227-bound alpha-synuclein fibrils Deposited 2022-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | IZM 5-[(~{E})-2-(6-methoxy-1,3-benzoxazol-2-yl)ethenyl]-~{N},~{N}-dimethyl-1,3-thiazol-2-amine × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 7YNQ PiB-bound alpha-synuclein fibrils conformation 2 Deposited 2022-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | IZV 2-[4-(methylamino)phenyl]-1,3-benzothiazol-6-ol × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 7YNR C05-03-bound alpha-synuclein fibrils Deposited 2022-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7YNS SIL5-bound alpha-synuclein fibrils Deposited 2022-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 7YNT pFTAA-bound alpha-synuclein fibrils Deposited 2022-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8A4L Lipidic alpha-synuclein fibril - polymorph L2A Deposited 2022-06-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 15 PDB declaration: pentadecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain O
1–140（140 aa）
Chain P
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.68 Å |
| 8A9L Cryo-EM structure of alpha-synuclein filaments from Parkinson's disease and dementia with Lewy bodies Deposited 2022-06-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: Trimeric |
Chain A
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å |
| 8ADS Lipidic alpha-synuclein fibril - polymorph L2B Deposited 2022-07-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.05 Å |
| 8ADU Lipidic alpha-synuclein fibril - polymorph L1A Deposited 2022-07-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.24 Å |
| 8ADV Lipidic alpha-synuclein fibril - polymorph L1B Deposited 2022-07-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.98 Å |
| 8ADW Lipidic alpha-synuclein fibril - polymorph L1C Deposited 2022-07-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.95 Å |
| 8AEX Lipidic alpha-synuclein fibril - polymorph L3A Deposited 2022-07-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.76 Å |
| 8BQV Cryo-EM structure of alpha-synuclein singlet filament from Juvenile-onset synucleinopathy Deposited 2023-01-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: Decameric |
Chain A
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.00 Å |
| 8BQW Cryo-EM structure of alpha-synuclein filaments doublet from Juvenile-onset synucleinopathy Deposited 2023-01-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: Eicosameric |
Chain A
1–140（140 aa）
Chain C
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.30 Å |
| 8CE7 Type1 alpha-synuclein filament assembled in vitro by wild-type and mutant (7 residues insertion) protein Deposited 2023-02-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: Hexameric |
Chain A
1–140（140 aa）
Chain C
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8CEB Type2 alpha-synuclein filament assembled in vitro by wild-type and mutant (7 residues insertion) protein Deposited 2023-02-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain C
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 8CYR Alpha-synuclein fibril from spontaneous control Deposited 2022-05-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Gibco Cat # 10010072
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 8CYS CryoEM structures of amplified alpha-synuclein fibril class B type II with extended core from DLB case I Deposited 2022-05-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 14 PDB declaration: tetradecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
Chain O
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Gibco Cat # 10010072
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8CYT CryoEM structures of amplified alpha-synuclein fibril class A type I with extended core from DLB case I Deposited 2022-05-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 14 PDB declaration: tetradecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
Chain O
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Gibco Cat # 10010072
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8CYV CryoEM structures of amplified alpha-synuclein fibril class B mixed type I/II with extended core from DLB case II Deposited 2022-05-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Gibco Cat # 10010072
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8CYW CryoEM structures of amplified alpha-synuclein fibril class B type I with compact core from DLB case III Deposited 2022-05-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Gibco Cat # 10010072
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8CYX CryoEM structure of amplified alpha-synuclein fibril class A type I with compact core from DLB case III Deposited 2022-05-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Gibco Cat # 10010072
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8CYY CryoEM structure of amplified alpha-synuclein fibril class B mixed type I/II with extended core from DLB case V Deposited 2022-05-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Gibco Cat # 10010072
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8CZ0 CryoEM structure of amplified alpha-synuclein fibril class B type I with extended core from DLB case VII Deposited 2022-05-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Gibco Cat # 10010072
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8CZ1 CryoEM structure of amplified alpha-synuclein fibril class B type II with extended core from DLB case VII Deposited 2022-05-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Gibco Cat # 10010072
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8CZ2 CryoEM structure of amplified alpha-synuclein fibril class A type I with extended core from DLB case VII Deposited 2022-05-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Gibco Cat # 10010072
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8CZ3 CryoEM structure of amplified alpha-synuclein fibril class B type I with extended core from DLB case X Deposited 2022-05-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Gibco Cat # 10010072
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8CZ6 CryoEM structure of amplified alpha-synuclein fibril class A type I with extended core from DLB case X Deposited 2022-05-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 14 PDB declaration: tetradecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
Chain O
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Gibco Cat # 10010072
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8FPT STRUCTURE OF ALPHA-SYNUCLEIN FIBRILS DERIVED FROM HUMAN LEWY BODY DEMENTIA TISSUE Deposited 2023-01-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
SOLID-STATE NMR
NMR measurement conditions
pH 7.6；273 K；Ionic strength (raw mmCIF value) 100 mM NACL；Pressure AMBIENT
NMR sample composition
66 % [U-100% 13C; U-100% 15N] ALPHA-SYNUCLEIN, 34% H2O | 34% H2O
NMR sample composition
66 % [U-100% 2H; U-100% 13C; U-100% 15N] ALPHA-SYNUCLEIN, 34% H2O, BACK-EXCHANGED IN 99.8% D2O | 34% H2O, BACK-EXCHANGED IN 99.8% D2O
NMR sample composition
20 % [U-25% 2-13C GLYCEROL; U-100% 15N] labelled ALPHA-SYNUCLEIN, 80 % ALPHA-SYNUCLEIN, H2O | H2O
|
Resolution not provided |
| 8G0L Semi-synthetic CoA-alpha-Synuclein Constructs Trap N-terminal Acetyltransferase NatB for Binding Mechanism Studies Deposited 2023-01-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
1–5（5 aa）
Fragment：UNP residues 1-5
|
Not recorded | CMC CARBOXYMETHYL COENZYME *A × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.39 Å |
| 8GF7 Cryo-EM structure of serine 87 O-GlcNAc-modified alpha-synuclein fibrils Deposited 2023-03-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
7–96（90 aa）
Chain B
7–96（90 aa）
Chain C
7–96（90 aa）
Chain D
7–96（90 aa）
Chain E
7–96（90 aa）
Chain F
7–96（90 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；normal PBS
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å |
| 8H03 Major polymorph in alpha-synuclein fibril seeded by cerebrospinal fluid from a preclinical Parkinson's disease patient Deposited 2022-09-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 8H04 Major polymorph in alpha-synuclein fibril seeded by cerebrospinal fluid from a postmortal Parkinson's disease patient Deposited 2022-09-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8H05 Minor polymorph in alpha-synuclein fibril seeded by cerebrospinal fluid from a postmortal Parkinson's disease patient Deposited 2022-09-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8HZB Formed alpha-synuclein fibrils after incubation with heparin for 1 hour (Hep-remod-1) Deposited 2023-01-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8HZC Formed alpha-synuclein fibrils after incubation with heparin for 1 hour (Hep-remod-2) Deposited 2023-01-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8HZS Formed alpha-synuclein fibrils after incubation with heparin for 3 days (Hep-remod-3) Deposited 2023-01-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8JEX Cryo-EM structure of alpha-synuclein gS87 fibril Deposited 2023-05-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain K
1–140（140 aa）
Chain P
1–140（140 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8JEY Cryo-EM structure of alpha-synuclein pS87 fibril Deposited 2023-05-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 8JJV Structure of truncated form of nanobody in complex with alpha-synuclein peptide Deposited 2023-05-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
43–56（14 aa）
|
Not recorded | GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1M BIS-TRIS pH 5.5, 2.0M ammonium sulfate
|
Resolution 1.23 Å R-free 0.170 |
| 8JLY Structure of nanobody in complex with alpha-synuclein peptide Deposited 2023-06-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
43–56（14 aa）
|
Not recorded | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0.1M sodium acetate trihydrate pH 4.6, 2.0M ammonium sulfate
|
Resolution 1.29 Å R-free 0.186 |
| 8OG0 Crystal structure of MJF14-6-4-2 Fab fragment in complex with epitope peptide Deposited 2023-03-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain P
136–140（5 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；Sitting droplets were set up by mixing 1 ul of Fab fragments, 0.5 ul of peptide (1 mg/ml in 20 mM tris-HCl) and 1 ul crystallization buffer (24% PEG 6000, 0.1M Na-citrate pH 5.0).
|
Resolution 1.71 Å R-free 0.231 |
| 8OQI Cryo-EM structure of the wild-type alpha-synuclein fibril. Deposited 2023-04-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8PIX Cryo EM structure of the type 3C polymorph of alpha-synuclein at low pH. Deposited 2023-06-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 5.8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.41 Å |
| 8PJO Cryo EM structure of the type 3D polymorph of alpha-synuclein E46K mutant at low pH. Deposited 2023-06-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K | CL CHLORIDE ION × 10 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 5.8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.31 Å |
| 8PK2 Cryo EM structure of the type 1m polymorph of alpha-synuclein Deposited 2023-06-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.26 Å |
| 8PK4 Cryo EM structure of the type 5A polymorph of alpha-synuclein. Deposited 2023-06-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.30 Å |
| 8QPZ CryoEM structure of recombinant DeltaN7 alpha-synuclein in PBS Deposited 2023-10-03 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: 12-meric |
Chain A
8–140（133 aa）
Chain B
8–140（133 aa）
Chain C
8–140（133 aa）
Chain D
8–140（133 aa）
Chain E
8–140（133 aa）
Chain F
8–140（133 aa）
Chain G
8–140（133 aa）
Chain H
8–140（133 aa）
Chain I
8–140（133 aa）
Chain J
8–140（133 aa）
Chain K
8–140（133 aa）
Chain L
8–140（133 aa）
|
Mutation：deltaN7 (methionine kept for expression) Mutation：deltaN7 (methionine kept for expression) Mutation：deltaN7 (methionine kept for expression) Mutation：deltaN7 (methionine kept for expression) Mutation：deltaN7 (methionine kept for expression) Mutation：deltaN7 (methionine kept for expression) Mutation：deltaN7 (methionine kept for expression) Mutation：deltaN7 (methionine kept for expression) Mutation：deltaN7 (methionine kept for expression) Mutation：deltaN7 (methionine kept for expression) Mutation：deltaN7 (methionine kept for expression) Mutation：deltaN7 (methionine kept for expression) | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4 and 1.5 mM KH2PO4; pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 8RI9 Late alpha-Synuclein fibril structure from liquid-liquid phase separations. Deposited 2023-12-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8RQM Alpha-synuclein amyloid fibrils Deposited 2024-01-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8RRR Alpha-synuclein amyloid fibril Deposited 2024-01-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8X7B ThT-bound E46K alpha-synuclein fibrils Deposited 2023-11-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | TFX 2-[4-(dimethylamino)phenyl]-3,6-dimethyl-1,3-benzothiazol-3-ium × 12 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8X7L EB-bound E46K alpha-synuclein fibrils Deposited 2023-11-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | IZ8 4-azanyl-6-[[4-[4-[(~{E})-(8-azanyl-1-oxidanyl-5,7-disulfo-naphthalen-2-yl)diazenyl]-3-methyl-phenyl]-2-methyl-phenyl]diazenyl]-5-oxidanyl-naphthalene-1,3-disulfonic acid × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8X7M CR-bound E46K alpha-synuclein fibrils Deposited 2023-11-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | CGO sodium 3,3'-(1E,1'E)-biphenyl-4,4'-diylbis(diazene-2,1-diyl)bis(4-aminonaphthalene-1-sulfonate) × 16 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8X7O PiB-bound E46K mutanted alpha-synuclein fibrils Deposited 2023-11-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K Mutation：E46K | IZV 2-[4-(methylamino)phenyl]-1,3-benzothiazol-6-ol × 12 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8X7P CCA-bound E46K alpha-synuclein fibrils Deposited 2023-11-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | V79 copper;trisodium;18-(2-carboxylatoethyl)-20-(carboxylatomethyl)-12-ethenyl-7-ethyl-3,8,13,17-tetramethyl-17,18-dihydroporphyrin-21,23-diide-2-carboxylate × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8X7Q pFTAA-bound E46K alpha-synuclein fibrils Deposited 2023-11-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | 3LS 3''',4'-bis(carboxymethyl)-2,2':5',2'':5'',2''':5''',2''''-quinquethiophene-5,5''''-dicarboxylic acid × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8X7R C05-03-bound E46K alpha-synuclein fibrils Deposited 2023-11-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | Y9W 2-[(~{E})-4-[6-(methylamino)pyridin-3-yl]but-1-en-3-ynyl]-1,3-benzothiazol-6-ol × 20 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8XWD Croy-EM structure of alpha synuclein fibril with EGCG Deposited 2024-01-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | KDH (2R,3R)-5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)-3,4-dihydro-2H-chromen-3-yl 3,4,5-trihydroxybenzoate × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8Y2P The cryo-EM structure of a-synuclein fibril in Tris buffer. Deposited 2024-01-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8Y2Q The cryo-EM structure of spermine induced a-synuclein fibril in Tris buffer. Deposited 2024-01-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 8ZLI BTA-2-bound E46K alpha-synuclein fibrils Deposited 2024-05-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
45–99（55 aa）
Chain B
45–99（55 aa）
Chain C
45–99（55 aa）
Chain D
45–99（55 aa）
Chain E
45–99（55 aa）
Chain F
45–99（55 aa）
Chain I
45–99（55 aa）
Chain J
45–99（55 aa）
Chain K
45–99（55 aa）
Chain L
45–99（55 aa）
|
Not recorded | A1L13 ~{N},~{N}-dimethyl-4-(6-methyl-1,3-benzothiazol-2-yl)aniline × 10 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8ZLO F0502B-bound E46K alpha-synuclein fibril Deposited 2024-05-20 | Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: 12-meric |
Chain A
45–99（55 aa）
Chain B
45–99（55 aa）
Chain C
45–99（55 aa）
Chain D
45–99（55 aa）
Chain E
45–99（55 aa）
Chain F
45–99（55 aa）
Chain G
45–99（55 aa）
Chain H
45–99（55 aa）
Chain I
45–99（55 aa）
Chain J
45–99（55 aa）
Chain O
45–99（55 aa）
Chain T
45–99（55 aa）
|
Not recorded | 1KI 2-bromanyl-4-[(~{E})-2-[6-[2-(2-fluoranylethoxy)ethyl-methyl-amino]-5-methyl-1,3-benzothiazol-2-yl]ethenyl]phenol × 10 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8ZLP apo WT polymorph 5a alpha-synuclein fibril Deposited 2024-05-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–98（98 aa）
Chain B
1–98（98 aa）
Chain C
1–98（98 aa）
Chain F
1–98（98 aa）
Chain G
1–98（98 aa）
Chain H
1–98（98 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8ZVY Alpha-Synuclein with H2a-H2b dimer complex structure. Deposited 2024-06-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
121–140（20 aa）
Chain D
121–140（20 aa）
|
Not recorded | CL CHLORIDE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291.15 K；100 mM Tris pH 8.0 and 10% PEG 8000.
|
Resolution 1.72 Å R-free 0.220 |
| 8ZWH Heparin bound E46K alpha-synuclein fibril Deposited 2024-06-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 8ZWI Chondroitin sulfate bound alpha-synuclein fibril Deposited 2024-06-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8ZWJ Polyadenylic acid bound alpha-synuclein fibril Polymorph 1 Deposited 2024-06-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Homooligomer；Protein × 6 PDB declaration: octameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain G
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8ZWK Polyadenylic acid bound alpha-synuclein fibril Polymorph 2 Deposited 2024-06-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–RNA Homooligomer；Protein × 6 PDB declaration: octameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9C5R Structure of recombinantly assembled alpha-synuclein variant fibrils Deposited 2024-06-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.61 Å |
| 9CD9 Structure of type II-2 alpha-synuclein filament from multiple system atrophy Deposited 2024-06-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 22 PDB declaration: 22-meric |
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
Chain O
1–140（140 aa）
Chain P
1–140（140 aa）
Chain Q
1–140（140 aa）
Chain R
1–140（140 aa）
Chain S
1–140（140 aa）
Chain T
1–140（140 aa）
Chain U
1–140（140 aa）
Chain V
1–140（140 aa）
Chain W
1–140（140 aa）
Chain X
1–140（140 aa）
Chain Y
1–140（140 aa）
Chain Z
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Wait time 30s, blot time 7.5s
|
Resolution 3.20 Å |
| 9CDA Structure of type I-2 alpha-synuclein filament from multiple system atrophy Deposited 2024-06-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 18 PDB declaration: 18-meric |
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
Chain O
1–140（140 aa）
Chain P
1–140（140 aa）
Chain Q
1–140（140 aa）
Chain R
1–140（140 aa）
Chain S
1–140（140 aa）
Chain T
1–140（140 aa）
Chain U
1–140（140 aa）
Chain V
1–140（140 aa）
Chain W
1–140（140 aa）
Chain X
1–140（140 aa）
Chain Y
1–140（140 aa）
Chain Z
1–140（140 aa）
Chain a
1–140（140 aa）
Chain b
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Wait time 30s, blot time 7.5s
|
Resolution 3.30 Å |
| 9CK3 Cryo-EM structure of in-vitro alpha-synuclein fibril Deposited 2024-07-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: 12-meric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.04 Å |
| 9CKK Cryo-EM structure of acetylated alpha-synuclein A53T fibril - polymorph A Deposited 2024-07-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.21 Å |
| 9CKL Cryo-EM structure of acetylated alpha-synuclein A53T fibril - polymorph B Deposited 2024-07-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.2；Dulbecco's Phosphate Buffered Saline without calcium chloride and magnesium chloride
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.68 Å |
| 9CX6 Cryo-EM filament structure in FTLD-synuclein Deposited 2024-07-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 20 PDB declaration: eicosameric |
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9CX6 Cryo-EM filament structure in FTLD-synuclein Deposited 2024-07-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9D5C Cryo-EM structure of serine 87 O-GlcNAc-modified alpha-synuclein fibrils Deposited 2024-08-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–96（96 aa）
Chain B
1–96（96 aa）
Chain C
1–96（96 aa）
Chain F
1–96（96 aa）
Chain G
1–96（96 aa）
Chain H
1–96（96 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；normal PBS
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å |
| 9E8U (Apo)Alpha-synuclein fibril structures from MSA patient brain Deposited 2024-11-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；30mM Tris-HCl, pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9E8V Complex fibril structure of MSA alpha-synuclein with CNS-11g at 4 hours Deposited 2024-11-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | A1BGB 2-(4-benzyl-1-oxophthalazin-2(1H)-yl)-N-(2,6-dimethylphenyl)acetamide × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；30mM Tris-HCl, pH 7.4, with 1% DMSO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9E8W Complex fibril structure of MSA alpha-synuclein with CNS-11g at 6 hours Deposited 2024-11-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | A1BGB 2-(4-benzyl-1-oxophthalazin-2(1H)-yl)-N-(2,6-dimethylphenyl)acetamide × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；30mM Tris-HCl, pH 7.4, with 1% DMSO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9E8X Complex fibril structure of MSA alpha-synuclein with CNS-11g at 10 hours Deposited 2024-11-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | A1BGB 2-(4-benzyl-1-oxophthalazin-2(1H)-yl)-N-(2,6-dimethylphenyl)acetamide × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；30mM Tris-HCl, pH 7.4, with 1% DMSO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9E8Y Complex fibril structure of MSA alpha-synuclein with CNS-11g at 15 hours Deposited 2024-11-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | A1BGB 2-(4-benzyl-1-oxophthalazin-2(1H)-yl)-N-(2,6-dimethylphenyl)acetamide × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；30mM Tris-HCl, pH 7.4, with 1% DMSO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9E9X Cryo-EM structure of alpha-synuclein filaments derived from the temporal cortex of the case of atypical multiple system atrophy Deposited 2024-11-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9EUU Structure of recombinant alpha-synuclein fibrils 1B capable of seeding GCIs in vivo Deposited 2024-03-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 18 PDB declaration: 18-meric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
Chain O
1–140（140 aa）
Chain P
1–140（140 aa）
Chain Q
1–140（140 aa）
Chain R
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；NaCl 100 mM, TRIS 20mM
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.93 Å |
| 9FYP Cryo EM structure of the type 3B polymorph of alpha-synuclein at low pH. Deposited 2024-07-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
Chain O
1–140（140 aa）
Chain P
1–140（140 aa）
Chain Q
1–140（140 aa）
Chain R
1–140（140 aa）
|
Not recorded | CL CHLORIDE ION × 10 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 5.8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.23 Å |
| 9HC6 The L2A-I alpha-synuclein fibril in the presence of MODAG-005 (short incubation) Deposited 2024-11-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 15 PDB declaration: 15-meric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
Chain O
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.79 Å |
| 9HC7 The L2A-II alpha-synuclein fibril in the presence of MODAG-005 (short incubation) Deposited 2024-11-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 15 PDB declaration: 15-meric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
Chain O
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.85 Å |
| 9HC8 The L2C alpha-synuclein fibril in the presence of MODAG-005 (short incubation) Deposited 2024-11-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.82 Å |
| 9HC9 The L2A-I alpha-synuclein fibril in the presence of MODAG-005 (long incubation) Deposited 2024-11-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 15 PDB declaration: 15-meric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
Chain O
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.76 Å |
| 9HCA The L2A-II alpha-synuclein fibril in the presence of MODAG-005 (long incubation) Deposited 2024-11-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 15 PDB declaration: 15-meric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
Chain O
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9HCB The L2C alpha-synuclein fibril in the presence of MODAG-005 (long incubation) Deposited 2024-11-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.95 Å |
| 9HGR In vitro grown WT Alpha-Synuclein Fibrils Deposited 2024-11-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.70 Å |
| 9HGS In vitro grown G14R Alpha-Synuclein Fibrils Deposited 2024-11-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.00 Å |
| 9HXA In vitro grown K58N Alpha-Synuclein Fibrils Deposited 2025-01-07 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–140（140 aa）
Chain C
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.70 Å |
| 9IC7 Cryo-EM structure of alpha-synuclein fibrils formed in artificial cerebrospinal fluid (aCSF) Deposited 2025-02-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Protein homooligomer Homooligomer；Protein × 14 PDB declaration: 14-meric |
Chain a
1–140（140 aa）
Chain b
1–140（140 aa）
Chain c
1–140（140 aa）
Chain d
1–140（140 aa）
Chain e
1–140（140 aa）
Chain f
1–140（140 aa）
Chain g
1–140（140 aa）
Chain h
1–140（140 aa）
Chain i
1–140（140 aa）
Chain j
1–140（140 aa）
Chain k
1–140（140 aa）
Chain l
1–140（140 aa）
Chain m
1–140（140 aa）
Chain n
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.33；Once all components were mixed together, the pH was adjusted by supplementing 1.6 microliters of 25% HCl to yield pH of 7.33.
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.90 Å |
| 9IJP Polyphosphate bound alpha-synuclein fibril Deposited 2024-06-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain E
1–140（140 aa）
Chain G
1–140（140 aa）
Chain I
1–140（140 aa）
Chain L
1–140（140 aa）
|
Not recorded | 7TT Tetraphosphate × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9JBY The cryo-EM structure of Ac-K58W_G51DA53T a-syn fibril. Deposited 2024-08-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
37–98（62 aa）
Chain B
37–98（62 aa）
Chain C
37–98（62 aa）
Chain D
37–98（62 aa）
Chain E
37–98（62 aa）
Chain F
37–98（62 aa）
|
Mutation：G51D,A53T,K58W Mutation：G51D,A53T,K58W Mutation：G51D,A53T,K58W Mutation：G51D,A53T,K58W Mutation：G51D,A53T,K58W Mutation：G51D,A53T,K58W | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.49 Å |
| 9JC3 The cryo-EM structure of Ac-G51DA53T P1 a-syn fibril. Deposited 2024-08-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
|
Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.39 Å |
| 9JC4 The cryo-EM structure of Ac-G51DA53T P2 a-syn fibril. Deposited 2024-08-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
|
Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.95 Å |
| 9JC5 The cryo-EM structure of Ac-G51DA53T P3 a-syn fibril. Deposited 2024-08-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.23 Å |
| 9JD7 The cryo-EM structure of Ac-G51DA53T a-syn fibril. Deposited 2024-08-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.57 Å |
| 9JD8 The cryo-EM structure of Ac-G51DA53T P5 a-syn fibril. Deposited 2024-08-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 9 PDB declaration: nonameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
|
Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.86 Å |
| 9JDK The cryo-EM structure of Ac-G51DA53T P6 a-syn fibril. Deposited 2024-08-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
37–99（63 aa）
Chain B
37–99（63 aa）
Chain C
37–99（63 aa）
Chain D
37–99（63 aa）
Chain E
37–99（63 aa）
Chain F
37–99（63 aa）
|
Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.32 Å |
| 9JE2 The cryo-EM structure of Ac-K58N_G51DA53T a-syn fibril. Deposited 2024-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
|
Mutation：K58N,G51D,A53T Mutation：K58N,G51D,A53T Mutation：K58N,G51D,A53T Mutation：K58N,G51D,A53T Mutation：K58N,G51D,A53T Mutation：K58N,G51D,A53T | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.06 Å |
| 9JE4 The cryo-EM structure of Ac-E57A_G51DA53T a-syn fibril. Deposited 2024-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
|
Mutation：E57A,G51D,A53T Mutation：E57A,G51D,A53T Mutation：E57A,G51D,A53T | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.37 Å |
| 9JE6 The cryo-EM structure of Ac-G51DA53T low salt a-syn fibril. Deposited 2024-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.43 Å |
| 9JE7 The cryo-EM structure of Ac-E57Q_G51DA53T a-syn fibril. Deposited 2024-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–140（140 aa）
Chain C
1–140（140 aa）
Chain E
1–140（140 aa）
|
Mutation：E57Q,G51D,A53T Mutation：E57Q,G51D,A53T Mutation：E57Q,G51D,A53T | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.36 Å |
| 9JE8 The cryo-EM structure of Ac-G51DA53T 30days a-syn fibril. Deposited 2024-09-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
|
Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 9JI8 Cryo-EM structure of alpha-synuclein-H21 fibril Deposited 2024-09-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain F
37–99（63 aa）
Fragment：UNP residues 37-99
Chain G
37–99（63 aa）
Fragment：UNP residues 37-99
Chain K
37–99（63 aa）
Fragment：UNP residues 37-99
Chain L
37–99（63 aa）
Fragment：UNP residues 37-99
Chain M
37–99（63 aa）
Fragment：UNP residues 37-99
Chain O
37–99（63 aa）
Fragment：UNP residues 37-99
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.85 Å |
| 9JKE The cryo-EM structure of Ac-K58W_G51DA53T P2 a-syn fibril. Deposited 2024-09-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
37–98（62 aa）
Chain B
37–98（62 aa）
Chain C
37–98（62 aa）
Chain D
37–98（62 aa）
Chain E
37–98（62 aa）
Chain F
37–98（62 aa）
|
Mutation：K58W,G51D,A53T Mutation：K58W,G51D,A53T Mutation：K58W,G51D,A53T Mutation：K58W,G51D,A53T Mutation：K58W,G51D,A53T Mutation：K58W,G51D,A53T | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9JO6 The complex of alpha-synuclein in the presence of PD6 Deposited 2024-09-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7.5；283.15 K；Ionic strength (raw mmCIF value) 0.1627；Pressure 1
NMR measurement conditions
pH 7.5；283.15 K；Ionic strength (raw mmCIF value) 0.1627；Pressure 1
NMR sample composition
0.50 mM [U-100% 13C; U-100% 15N] alpha-synuclein, 1.00 mM PD6, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.20 mM alpha-synuclein, 0.40 mM [U-100% 13C; U-100% 15N] PD6, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9K7Q Parkinson disease protein 7 (DJ-1) and Alpha-synuclein (Alpha-syn) complex Deposited 2024-10-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain M
1–92（92 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2 M Sodium iodide, 20% w/v Polyethylene glycol 3,350, pH 7.0
|
Resolution 4.58 Å R-free 0.222 |
| 9KA3 The cryo-EM structure of Ac-K58D_G51DA53T a-syn fibril. Deposited 2024-10-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Mutation：K58D,G51D,A53T Mutation：K58D,G51D,A53T Mutation：K58D,G51D,A53T Mutation：K58D,G51D,A53T Mutation：K58D,G51D,A53T Mutation：K58D,G51D,A53T | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.72 Å |
| 9KA4 The cryo-EM structure of MSA-like fold (P1) formed under the palette stratagy. Deposited 2024-10-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.42 Å |
| 9KAL The cryo-EM structure of Ac-E57A_G51DA53T a-syn fibril. Deposited 2024-10-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
|
Mutation：E57A,G51D,A53T Mutation：E57A,G51D,A53T Mutation：E57A,G51D,A53T | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.36 Å |
| 9KC2 Cryo-EM structure of ZSQ07-bound alpha-synuclein fibril polymorph 6A6B Deposited 2024-10-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
|
Not recorded | A1EFK 2-[(4~{E})-4-[(~{E})-3-(3-methyl-1,3-benzothiazol-2-yl)prop-2-enylidene]quinolin-1-yl]ethanol × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9KCN Cryo-EM structure of FD4-bound alpha-synuclein fibril polymorph 6A6B Deposited 2024-11-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric |
Chain L
37–140（104 aa）
Chain M
37–140（104 aa）
Chain N
37–140（104 aa）
Chain P
37–140（104 aa）
Chain Q
37–140（104 aa）
Chain R
37–140（104 aa）
Chain d
37–140（104 aa）
Chain e
37–140（104 aa）
Chain f
37–140（104 aa）
Chain h
37–140（104 aa）
Chain i
37–140（104 aa）
Chain j
37–140（104 aa）
|
Not recorded | A1EFL (2~{R})-1-fluoranyl-3-[[2-[(~{E})-2-[5-[6-(methylamino)pyridin-3-yl]pyridin-2-yl]ethenyl]-1,3-benzothiazol-6-yl]oxy]propan-2-ol × 12 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9KCO Cryo-EM structure of FD4-bound alpha-synuclein MSA-like fibril Deposited 2024-11-01 | Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
Chain O
1–140（140 aa）
Chain P
1–140（140 aa）
Chain Q
1–140（140 aa）
|
Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T Mutation：G51D,A53T | A1EFL (2~{R})-1-fluoranyl-3-[[2-[(~{E})-2-[5-[6-(methylamino)pyridin-3-yl]pyridin-2-yl]ethenyl]-1,3-benzothiazol-6-yl]oxy]propan-2-ol × 12 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9KCP Cryo-EM structure of ACI-12589-bound alpha-synuclein fibril polymorph 6A6B Deposited 2024-11-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9M5K The cryo-EM structure of in situ amplified (ISA) alpha-synuclein fibrils from PD homogenate Deposited 2025-03-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9M5L The cryo-EM structure of in situ amplified (ISA) alpha-synuclein fibrils from DLB homogenate Deposited 2025-03-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.27 Å |
| 9O4B Cryo-EM structure of in-vitro alpha-synuclein fibril bound with Exemplar-6 PET-radioligand Deposited 2025-04-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: 12-meric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
|
Not recorded | A1B9H 2-(3,4-dimethylphenoxy)-N-[3-(4-fluorophenyl)-1,2-oxazol-5-yl]acetamide × 12 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.59 Å |
| 9O9J Cryo-EM structure of alpha-synuclein filaments from Parkinson's disease with G51D mutaion in SNCA Deposited 2025-04-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: 20-meric |
Chain A
1–140（140 aa）
Chain D
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.30 Å |
| 9O9K Cryo-EM structure of alpha-synuclein filaments from Parkinson's disease with H50Q variant in SNCA Deposited 2025-04-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: 15-meric |
Chain A
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9OBP Cryo-EM structure of alpha-synuclein filaments derived from the frontal cortex of the case of atypical multiple system atrophy Deposited 2025-04-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 9QYL Alpha-synuclein fibril type 1A polymorph in the presence of ATP Deposited 2025-04-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 24 PDB declaration: 24-meric |
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
Chain O
1–140（140 aa）
Chain P
1–140（140 aa）
Chain Q
1–140（140 aa）
Chain e
1–140（140 aa）
Chain f
1–140（140 aa）
Chain g
1–140（140 aa）
Chain h
1–140（140 aa）
Chain j
1–140（140 aa）
Chain k
1–140（140 aa）
Chain l
1–140（140 aa）
Chain m
1–140（140 aa）
Chain n
1–140（140 aa）
Chain o
1–140（140 aa）
Chain p
1–140（140 aa）
Chain q
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；20 mM Tris, 150 mM NaCl, pH 7.5, 1 mM MgCl2, 1 mM ATP
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.08 Å |
| 9QYN Alpha-synuclein K21A fibril type 1A polymorph in the presence of ATP Deposited 2025-04-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
Chain O
1–140（140 aa）
Chain P
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；20 mM Tris, 150 mM NaCl, pH 7.5, 1 mM MgCl2, 1 mM ATP
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.13 Å |
| 9RB3 A53T alpha-synuclein fibril - Type 1 Deposited 2025-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9RB6 A53T alpha-synuclein fibril - Type 2 Deposited 2025-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Mutation：A53T Mutation：A53T Mutation：A53T Mutation：A53T Mutation：A53T Mutation：A53T Mutation：A53T Mutation：A53T Mutation：A53T Mutation：A53T | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.02 Å |
| 9RB7 Wild-type alpha-synuclein fibril - Type 1 Deposited 2025-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.45 Å |
| 9RB8 Wild-type alpha-synuclein fibril - Type 3-1 Deposited 2025-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.64 Å |
| 9RB9 Wild-type alpha-synuclein fibril - Type 3-2 Deposited 2025-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.04 Å |
| 9RBA Wild-type alpha-synuclein fibril - Type 4 Deposited 2025-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.54 Å |
| 9RBB Wild-type alpha-synuclein fibril - Type 5 Deposited 2025-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.15 Å |
| 9RZF Structure of in-vivo formed alpha-synuclein fibrils purified from a M83+/- mouse brain injected with recombinant 1B fibrils Deposited 2025-07-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
37–97（61 aa）
Chain B
37–97（61 aa）
Chain C
37–97（61 aa）
Chain D
37–97（61 aa）
Chain E
37–97（61 aa）
Chain F
37–97（61 aa）
|
Mutation：A53T Mutation：A53T Mutation：A53T Mutation：A53T Mutation：A53T Mutation：A53T | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；50 mM Tris-HCl, 150 mM NaCl, pH7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 9TPT Structure of recombinantly assembled E83Q alpha-synuclein fibrils Deposited 2025-12-18 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: 12-meric |
Chain A
36–100（65 aa）
Chain B
36–100（65 aa）
Chain C
36–100（65 aa）
Chain D
36–100（65 aa）
Chain E
36–100（65 aa）
Chain F
36–100（65 aa）
Chain H
36–100（65 aa）
Chain I
36–100（65 aa）
Chain J
36–100（65 aa）
Chain L
36–100（65 aa）
Chain M
36–100（65 aa）
Chain N
36–100（65 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.43 Å |
| 9UF6 Cryo-EM structure of BA1-1-bound alpha-synuclein fibril polymorph 6A6B Deposited 2025-04-10 | Different construct Different mutation/modification Different ligand/ion | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
Chain O
1–140（140 aa）
Chain P
1–140（140 aa）
|
Not recorded | A1EO7 (2~{E})-1-ethyl-2-[(~{E})-3-(1-ethylquinolin-1-ium-2-yl)prop-2-enylidene]quinoline × 24 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9UFD Cryo-EM structure of BA12-bound alpha-synuclein fibril polymorph 6A6B Deposited 2025-04-10 | Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
Chain M
1–140（140 aa）
Chain N
1–140（140 aa）
Chain O
1–140（140 aa）
Chain P
1–140（140 aa）
|
Not recorded | A1EO8 (2~{Z})-3-ethyl-2-[(~{E})-3-(3-ethyl-1,3-benzothiazol-3-ium-2-yl)prop-2-enylidene]-1,3-benzothiazole × 36 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9UFF Cryo-EM structure of BA21-bound alpha-synuclein fibril polymorph 6A6B Deposited 2025-04-10 | Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
|
Not recorded | A1EO6 ~{N}-(1-ethyl-2-oxidanylidene-benzo[cd]indol-6-yl)-2,5-dimethoxy-benzamide × 12 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9UFL Cryo-EM structure of BA22-bound alpha-synuclein fibril polymorph 6A6B Deposited 2025-04-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9UFS Cryo-EM structure of BA23-bound alpha-synuclein fibril polymorph 6A6B Deposited 2025-04-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9UFX Cryo-EM structure of HA11-bound alpha-synuclein fibril polymorph 6A6B Deposited 2025-04-10 | Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
|
Not recorded | A1EPA 2',4',5',7'-tetrakis(iodanyl)-3',6'-bis(oxidanyl)spiro[2-benzofuran-3,9'-xanthene]-1-one × 12 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9UFY Cryo-EM structure of HA21-bound alpha-synuclein fibril polymorph 6A6B Deposited 2025-04-10 | Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
|
Not recorded | GO3 Gossypol × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9UG0 Cryo-EM structure of HA31-bound alpha-synuclein fibril polymorph 6A6B Deposited 2025-04-10 | Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
|
Not recorded | A1EPK 4-[(3~{R},5~{S})-3,5-dimethylpiperidin-1-yl]sulfonyl-~{N}'-(4-methyl-1,3-benzothiazol-2-yl)benzohydrazide × 12 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9UG1 Cryo-EM structure of HA32-bound alpha-synuclein fibril polymorph 6A6B Deposited 2025-04-10 | Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
Chain K
1–140（140 aa）
Chain L
1–140（140 aa）
|
Not recorded | A1EPB 4-(3,4-dihydro-1~{H}-isoquinolin-2-ylsulfonyl)-~{N}'-(4-methyl-1,3-benzothiazol-2-yl)benzohydrazide × 12 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9UJQ Cryo-EM structure of HQY1027-bound alpha-synuclein fibril polymorph 6A6B Deposited 2025-04-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion | Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9V6U The cryo-EM structure of FD4 bound Lewy fold fibril. Deposited 2025-05-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 15 PDB declaration: pentadecameric |
Chain c
1–140（140 aa）
Chain d
1–140（140 aa）
Chain e
1–140（140 aa）
Chain f
1–140（140 aa）
Chain g
1–140（140 aa）
Chain h
1–140（140 aa）
Chain i
1–140（140 aa）
Chain j
1–140（140 aa）
Chain k
1–140（140 aa）
Chain o
1–140（140 aa）
Chain p
1–140（140 aa）
Chain q
1–140（140 aa）
Chain r
1–140（140 aa）
Chain s
1–140（140 aa）
Chain t
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions | Resolution 3.30 Å |
| 9V7C The cryo-EM structure of FD4_orientation2 bound Lewy fold fibril. Deposited 2025-05-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 15 PDB declaration: pentadecameric |
Chain c
32–101（70 aa）
Chain d
32–101（70 aa）
Chain e
32–101（70 aa）
Chain f
32–101（70 aa）
Chain g
32–101（70 aa）
Chain h
32–101（70 aa）
Chain i
32–101（70 aa）
Chain j
32–101（70 aa）
Chain k
32–101（70 aa）
Chain o
32–101（70 aa）
Chain p
32–101（70 aa）
Chain q
32–101（70 aa）
Chain r
32–101（70 aa）
Chain s
32–101（70 aa）
Chain t
32–101（70 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions | Resolution 3.30 Å |
| 9Y0S CryoEM structure of alpha-synuclein fibril induced by psychosine Deposited 2025-08-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 10 PDB declaration: decameric |
Chain A
1–140（140 aa）
Chain B
1–140（140 aa）
Chain C
1–140（140 aa）
Chain D
1–140（140 aa）
Chain E
1–140（140 aa）
Chain F
1–140（140 aa）
Chain G
1–140（140 aa）
Chain H
1–140（140 aa）
Chain I
1–140（140 aa）
Chain J
1–140（140 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；20 mM Tris-HCl, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.72 Å |
226 other PDB entries and 234 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | SYUA_HUMAN |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 2–99； UniProt 1–98 Author chain B； PDBConstruct 2–99； UniProt 1–98 Author chain C； PDBConstruct 2–99； UniProt 1–98 Author chain D； PDBConstruct 2–99； UniProt 1–98 Author chain E； PDBConstruct 2–99； UniProt 1–98 Author chain F； PDBConstruct 2–99； UniProt 1–98 Author chain G； PDBConstruct 2–99； UniProt 1–98 Author chain H； PDBConstruct 2–99； UniProt 1–98 Author chain I； PDBConstruct 2–99； UniProt 1–98 Author chain J； PDBConstruct 2–99； UniProt 1–98 Author chain K； PDBConstruct 2–99； UniProt 1–98 Author chain L； PDBConstruct 2–99； UniProt 1–98 |
