当前蛋白身份：P03856
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相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1REP CRYSTAL STRUCTURE OF REPLICATION INITIATOR PROTEIN REPE54 OF MINI-F PLASMID COMPLEXED WITH AN ITERON DNA 提交 1999-04-29 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8；12 % PEG 400, 200 MM MGCL2, 100 MM TRIS-HCL, PH8.0, VAPOR DIFFUSION, HANGING DROP
|
分辨率 2.60 Å R-free 0.274 |
| 2Z9O Crystal structure of the dimeric form of RepE in complex with the repE operator DNA 提交 2007-09-21 | Assembly 1 蛋白–DNA 同源多聚体；蛋白 × 2 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 A
2–251（250 aa）
链 B
2–251（250 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7.2；277 K；10% PEG 4000, 200mM Sodium Chloride, 5mM Samarium Chloride, 100mM HEPES-NaOH, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
分辨率 3.14 Å R-free 0.313 |
| 7RVA Updated Crystal Structure of Replication Initiator Protein REPE54. 提交 2021-08-18 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | NA SODIUM ION × 1 MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；300 mM MgCl2, 18% PEG 400, and 100 mM Tris HCl
|
分辨率 1.89 Å R-free 0.249 |
| 7U6K Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence. 提交 2022-03-04 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 7 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；400 mM MgCl2, 24% PEG 400, and 80 mM Tris HCl
|
分辨率 2.38 Å R-free 0.224 |
| 7U7O Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional T-A rich sequence. 提交 2022-03-07 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；300 mM MgCl2, 28% PEG 400, and 80 mM Tris HCl
|
分辨率 2.97 Å R-free 0.258 |
| 7UFX Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and scaffold-insert duplexes (21mer and 10mer) containing the cognate REPE54 sequence and an additional G-C rich sequence, respectively. 提交 2022-03-23 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：pentameric（5） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；180 mM MgCl2, 20% PEG 400, and 100 mM Tris HCl
|
分辨率 2.80 Å R-free 0.226 |
| 7UOG Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and asymmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence. 提交 2022-04-12 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；200 mM MgCl2, 30% PEG 400, and 100 mM Tris HCl
|
分辨率 2.63 Å R-free 0.221 |
| 7UR0 Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and scaffold-insert duplexes (21mer and 10mer) containing the cognate REPE54 sequence and an additional T-A rich sequence, respectively. 提交 2022-04-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：pentameric（5） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；120 mM MgCl2, 20% PEG 400, 100 mM Tris HCl pH 8.0
|
分辨率 3.30 Å R-free 0.275 |
| 7UV6 Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and scaffold duplex (21mer) containing the cognate REPE54 sequence and an insert duplex (10mer) with guest TAMRA-labelled thymine and G-C rich sequence. 提交 2022-04-29 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：pentameric（5） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；300 mM MgCl2, 15% PEG 400, and 100 mM Tris HCl
|
分辨率 2.72 Å R-free 0.224 |
| 7UV7 Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and scaffold duplex (21mer) containing the cognate REPE54 sequence and an insert duplex (10mer) with guest TAMRA-labelled thymine and T-A rich sequence. 提交 2022-04-29 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：pentameric（5） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；200 mM MgCl2, 30% PEG 400, 100 mM Tris HCl pH 8.0
|
分辨率 3.02 Å R-free 0.269 |
| 7UXY Isoreticular, interpenetrating co-crystal of protein variant Replication Initiator Protein REPE54 (L53G,Q54G,E55G) and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence. 提交 2022-05-06 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P,L53G,Q54G,E55G | MG MAGNESIUM ION × 8 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；350 mM MgCl2, 30% PEG 400, and 100 mM Tris HCl
|
分辨率 3.15 Å R-free 0.264 |
| 8AAN The nucleoprotein complex of Rep protein with iteron containing dsDNA and DUE ssDNA. 提交 2022-07-01 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 A
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；291.15 K；10% w/v PEG 20 000, 20% v/v PEG MME 550 0.02M of each carboxylic acid (0.2 M sodium formate, 0.2M ammonium acetate, 0.2M trisodium citrate, 0.2M sodium potassium L-tartrate, 0.2M sodium oxamate), 0.1M MES/imidazole pH 6.5.
|
分辨率 2.19 Å R-free 0.233 |
| 8AC8 The nucleoprotein complex of Rep protein with DUE ssDNA 提交 2022-07-05 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：dimeric（2） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；291.15 K；PEG 8000, ethylene glycol, HEPES, halogens
|
分辨率 1.60 Å R-free 0.243 |
| 8AC8 The nucleoprotein complex of Rep protein with DUE ssDNA 提交 2022-07-05 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：dimeric（2） 与全部聚合物一致 |
链 A
1–251（251 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；291.15 K；PEG 8000, ethylene glycol, HEPES, halogens
|
分辨率 1.60 Å R-free 0.243 |
| 8D86 Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence co-crystallized with a guest small molecule, netropsin. 提交 2022-06-07 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 5 NT NETROPSIN × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；300 mM MgCl2, 25% PEG 400, and 100 mM Tris HCl
|
分辨率 3.12 Å R-free 0.234 |
| 8D8M Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence grown in a calcium chloride crystallization solution. 提交 2022-06-08 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；400 mM MgCl2, 24% PEG 400, and 80 mM Tris HCl
|
分辨率 3.10 Å R-free 0.255 |
| 8TIP Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with blunt ends and 5' terminal phosphates. 提交 2023-07-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；400 mM MgCl2, 25% PEG 400, and 100 mM Tris HCl
|
分辨率 2.79 Å R-free 0.240 |
| 8TIQ Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with blunt ends and 3' terminal phosphates. 提交 2023-07-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；350 mM MgCl2, 25% PEG 400, and 100 mM Tris HCl
|
分辨率 2.45 Å R-free 0.267 |
| 8TIR Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 1 sticky bases and 5' terminal phosphates. 提交 2023-07-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；350 mM MgCl2, 30% PEG 400, and 100 mM Tris HCl
|
分辨率 3.11 Å R-free 0.242 |
| 8TIS Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 1 sticky bases and 3' terminal phosphates. 提交 2023-07-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；350 mM MgCl2, 25% PEG 400, and 100 mM Tris HCl
|
分辨率 2.54 Å R-free 0.278 |
| 8TIT Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 2 sticky base overhangs and no terminal phosphates. 提交 2023-07-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；450 mM MgCl2, 25% PEG 400, and 100 mM Tris HCl
|
分辨率 2.84 Å R-free 0.240 |
| 8TIU Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 2 sticky base overhangs and 3' terminal phosphates. 提交 2023-07-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；400 mM MgCl2, 30% PEG 400, and 100 mM Tris HCl
|
分辨率 3.90 Å R-free 0.231 |
| 8TIV Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with blunt ends and 5' terminal phosphates and crosslinked with EDC. 提交 2023-07-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；350 mM MgCl2, 25% PEG 400, and 100 mM Tris HCl
|
分辨率 3.35 Å R-free 0.260 |
| 8TIW Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with blunt ends and 3' terminal phosphates and crosslinked with EDC. 提交 2023-07-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；350 mM MgCl2, 25% PEG 400, and 100 mM Tris HCl
|
分辨率 3.11 Å R-free 0.212 |
| 8TIX Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 1 sticky bases and 5' terminal phosphates and crosslinked with EDC. 提交 2023-07-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；350 mM MgCl2, 30% PEG 400, and 100 mM Tris HCl
|
分辨率 2.91 Å R-free 0.228 |
| 8TIY Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 1 sticky bases and 3' terminal phosphates and crosslinked with EDC. 提交 2023-07-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；350 mM MgCl2, 25% PEG 400, and 100 mM Tris HCl
|
分辨率 3.11 Å R-free 0.231 |
| 8TIZ Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 2 sticky base overhangs and no terminal phosphates and crosslinked with EDC. 提交 2023-07-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；450 mM MgCl2, 25% PEG 400, and 100 mM Tris HCl
|
分辨率 3.11 Å R-free 0.217 |
| 8TJ0 Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 2 sticky base overhangs and 5' terminal phosphates and crosslinked with EDC. 提交 2023-07-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；400 mM MgCl2, 24% PEG 400, and 80 mM Tris HCl
|
分辨率 3.24 Å R-free 0.225 |
| 8TJ1 Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 2 sticky base overhangs and 3' terminal phosphates and crosslinked with EDC. 提交 2023-07-20 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：R118P | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；400 mM MgCl2, 30% PEG 400, and 100 mM Tris HCl
|
分辨率 3.15 Å R-free 0.225 |
| 9NB0 Isoreticular, Porous co-crystal of Replication Initiator Protein REPE54 and asymmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and additional expansion sequence 提交 2025-02-13 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；500mM Lithium Sulfate, 20mM Magnesium Acetate, 50mM MES
|
分辨率 3.20 Å R-free 0.197 |
| 9NCR Isoreticular, Porous co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and additional G/C rich expansion sequence 提交 2025-02-17 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；1M lithium sulfate, 30mM Magnesium acetate, 50mM MES
|
分辨率 3.24 Å R-free 0.231 |
| 9YZA Isoreticular co-crystal 1 of protein variant Replication Initiator Protein REPE54 (L53G, Q54G, E55G) with symmetrical expanded duplex (31mer) containing insert sequence CCCGGCCGGA 提交 2025-10-30 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
突变：L53G, Q54G, E55G | MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；10 mM Magnesium acetate, 1300 mM Lithium sulfate, 50 mM MES pH 6.5
|
分辨率 3.05 Å R-free 0.309 |
| 9YZB Isoreticular co-crystal 1 with symmetrical expanded duplex (31mer) containing insert sequence ACGGTAATTA 提交 2025-10-30 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；10 mM Magnesium acetate, 1.6M Lithium sulfate, 50 mM MES pH 6.5
|
分辨率 4.13 Å R-free 0.253 |
| 9YZC Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence CGCGCGCGCG 提交 2025-10-30 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；80 mM Magnesium acetate, 1.2 M Lithium sulfate, 50 mM MES pH 6.5
|
分辨率 3.16 Å R-free 0.299 |
| 9YZD Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence CTAATTAGGC 提交 2025-10-30 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；30 mM Magnesium acetate, 1.3 M Lithium sulfate, 50 mM MES pH 6.5
|
分辨率 3.09 Å R-free 0.265 |
| 9YZE Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence TGATGAGCAG 提交 2025-10-30 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；20 mM Magnesium acetate, 300 mM Lithium sulfate, 50 mM MES pH 6.5
|
分辨率 4.07 Å R-free 0.276 |
| 9YZF Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence AATTAGGCCG 提交 2025-10-30 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；50 mM Magnesium acetate, 1.1 M Lithium sulfate, 50 mM MES pH 6.5
|
分辨率 3.07 Å R-free 0.241 |
| 9YZG Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence ATGAGTCATA 提交 2025-10-30 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；20 mM Magnesium acetate, 1.4 M Lithium sulfate, 50 mM MES pH 6.5
|
分辨率 3.09 Å R-free 0.301 |
| 9YZI Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence CCCGGCCGGA 提交 2025-10-30 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；50 mM Magnesium acetate, 1.6 M Lithium sulfate, 50 mM MES pH 6.5
|
分辨率 4.00 Å R-free 0.322 |
| 9YZJ Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence CGTAATTAGG 提交 2025-10-30 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；30 mM Magnesium acetate, 1.7 M Lithium sulfate, 50 mM MES pH 6.5
|
分辨率 2.97 Å R-free 0.292 |
| 9YZK Isoreticular co-crystal 1 with symmetrical expanded duplex (42mer) containing insert sequence ACCCTTCTATGACCTACTCCA 提交 2025-10-30 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；298 K；60mM Magnesium acetate, 1.6M Lithium sulfate, 50mM MES pH 6.5
|
分辨率 5.10 Å R-free 0.304 |
| 9YZL Isoreticular co-crystal of Replication Initiator Protein REPE54 and asymmetrical expanded duplex (31mer) containing the cognate REPE54 sequence, and additional T-A rich sequence with 5' terminal phosphates. Two copies of Even-skipped homeodomain loaded post-crystallization 提交 2025-10-30 | Assembly 1 蛋白–DNA 异源复合物；蛋白 × 3 PDB 声明：pentameric（5） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；30mM Magnesium Acetate, 1.2M Lithium sulfate, 50mM MES pH 6.5. The crystal was crosslinked with 33mg/mL EDC overnight, and then looped into a solution of 15mM potassium chloride, 4mM calcium chloride, 10% glycerol, and 10mM Tris hydrochloride for 1 hour. The drop was then supplemented with 30 micromolar Even-skipped homeodomain
|
分辨率 3.02 Å R-free 0.294 |
| 9YZM Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence CTAATTAGGC and loaded with Even-skipped homeodomain 提交 2025-10-30 | Assembly 1 蛋白–DNA 异源复合物；蛋白 × 3 PDB 声明：pentameric（5） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；30mM magnesium acetate, 1.1M lithium sulfate, 50mM MES pH 6.5. The crystal was crosslinked with 33mg/mL EDC overnight, and then looped into a solution of 50mM potassium chloride, 4mM calcium chloride, 10% glycerol, and 10mM Tris hydrochloride for 1 hour. The drop was then supplemented with 30 micromolar Even-skipped homeodomain.
|
分辨率 3.07 Å R-free 0.269 |
| 9YZN Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence TGATGAGCAG and loaded with Even-skipped homeodomain 提交 2025-10-30 | Assembly 1 蛋白–DNA 异源复合物；蛋白 × 3 PDB 声明：pentameric（5） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；40mM Magnesium acetate, 1.4M Lithium sulfate, 50mM MES pH 6.5. The crystal was crosslinked with 33mg/mL EDC overnight, and then looped into a solution of 25mM potassium chloride, 4mM calcium chloride, 10% glycerol, and 10mM Tris hydrochloride for 1 hour. The drop was then supplemented with 30 micromolar Even-skipped homeodomain.
|
分辨率 3.16 Å R-free 0.283 |
| 9YZO Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence TGATGAGCAG and loaded with Even-skipped homeodomain 提交 2025-10-30 | Assembly 1 蛋白–DNA 异源复合物；蛋白 × 3 PDB 声明：pentameric（5） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；20mM magnesium acetate, 1.7M lithium sulfate, 50mM MES pH 6.5. The crystal was crosslinked with 33mg/mL EDC overnight, and then looped into a solution of 50mM potassium chloride, 4mM calcium chloride, 10% glycerol, and 10mM Tris hydrochloride for 1 hour. The drop was then supplemented with 30 micromolar Even-skipped homeodomain.
|
分辨率 2.96 Å R-free 0.312 |
| 9YZP Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence TGATGAGCAG and loaded with ultrabithorax homeodomain 提交 2025-10-30 | Assembly 1 蛋白–DNA 异源复合物；蛋白 × 2 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；40mM Magnesium acetate, 1.5M Lithium sulfate, 50mM MES pH 6.5. The crystal was crosslinked with 45mg/mL EDC overnight, and then looped into a solution of 50mM potassium chloride, 4mM calcium chloride, 10% glycerol, and 10mM Tris hydrochloride for 1 hour. The drop was then supplemented with 40 micromolar Ultrabithorax homeodomain.
|
分辨率 3.67 Å R-free 0.295 |
| 9YZQ Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence TGATGAGCAG and loaded with Engrailed homeodomain enhanced Green fluorescent protein fusion 提交 2025-10-30 | Assembly 1 信息不足 异源复合物；蛋白 × 2 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；40mM Magnesium acetate, 1.4M Lithium sulfate, 50mM MES pH 6.5. The crystal was crosslinked with 45mg/mL EDC overnight, and then looped into a solution of 50mM potassium chloride, 4mM calcium chloride, 10% glycerol, and 10mM Tris hydrochloride for 1 hour. The drop was then supplemented with 22 micromolar engrailed homeodomain-enhanced Green fluorescent protein fusion
|
分辨率 3.75 Å R-free 0.338 |
| 9YZR Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence TAATTAGGCCG and loaded with ultrabithorax homeodomain 提交 2025-10-30 | Assembly 1 蛋白–DNA 异源复合物；蛋白 × 3 PDB 声明：pentameric（5） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；30mM magnesium acetate, 900mM Lithium sulfate, 50mM MES pH 6.5. The crystal was crosslinked with 48mg/mL EDC overnight, and then looped into a solution of 35mM potassium chloride, 4mM calcium chloride, 10% glycerol, and 10mM Tris hydrochloride for 1 hour. The drop was then supplemented with 80 micromolar Ultrabithorax homeodomain.
|
分辨率 3.39 Å R-free 0.298 |
| 9YZS Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence TAATTAGGCCG and loaded with Even-skipped homeodomain 提交 2025-10-30 | Assembly 1 蛋白–DNA 异源复合物；蛋白 × 3 PDB 声明：pentameric（5） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；30mM magnesium acetate, 800mM lithium sulfate, 50mM MES pH 6.5. The crystal was crosslinked with 47.5mg/mL EDC overnight, and then looped into a solution of 50mM potassium chloride, 4mM calcium chloride, 10% glycerol, and 10mM Tris hydrochloride for 1 hour. The drop was then supplemented with 30 micromolar Even-skipped homeodomain.
|
分辨率 3.08 Å R-free 0.291 |
| 9YZT Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence TAATTAGGCCG and loaded with Antennapedia homeodomain 提交 2025-10-30 | Assembly 1 蛋白–DNA 异源复合物；蛋白 × 3 PDB 声明：pentameric（5） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；30mM Magnesium acetate, 1.1M Lithium sulfate, 50mM MES pH 6.5. The crystal was crosslinked with 47.5mg/mL EDC overnight, and then looped into a solution of 50mM potassium chloride, 4mM calcium chloride, 10% glycerol, and 10mM Tris hydrochloride for 1 hour. The drop was then supplemented with 23 micromolar Even-skipped homeodomain.
|
分辨率 3.30 Å R-free 0.292 |
| 9YZU Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence ATGAGTCATA and loaded with mutated Bzip region of GCN4 transcription factor 提交 2025-10-30 | Assembly 1 蛋白–DNA 异源复合物；蛋白 × 4 PDB 声明：hexameric（6） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；50mM magnesium acetate, 1.8M lithium sulfate, 50mM MES pH 6.5. The crystal was crosslinked with 50mg/mL EDC overnight, and then looped into a solution of 50mM potassium chloride, 4mM calcium chloride, 10% glycerol, and 10mM Tris hydrochloride for 1 hour. The drop was then supplemented with 33 micromolar bZip
|
分辨率 3.05 Å R-free 0.246 |
| 9Z08 Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing separate insert strand with sequence GACGGTAATT 提交 2025-10-31 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：pentameric（5） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；10 mM Magnesium acetate, 1.3 M Lithium sulfate, 50 mM sodium cacodylate pH 6.5
|
分辨率 4.22 Å R-free 0.254 |
| 9Z1A Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence CCGCGCAGGC 提交 2025-11-03 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；80 mM Magnesium Acetate, 1.2 M Lithium Sulfate, 50 mM MES pH 6.5
|
分辨率 3.10 Å R-free 0.335 |
| 9Z1B Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence CCGCGCAGGC, crystal soaked in alternate solvent prior to diffraction 提交 2025-11-03 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | CA CALCIUM ION × 2 K POTASSIUM ION × 3 MG MAGNESIUM ION × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；50mM Magnesium acetate, 1.6M Lithium sulfate, 50mM MES pH 6.5. The crystal was crosslinked with 40 mg/mL EDC overnight, and then looped into a solution of 50mM potassium chloride, 4mM calcium chloride, 10% glycerol, and 10mM Tris hydrochloride.
|
分辨率 3.88 Å R-free 0.308 |
| 9Z1C Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence CTAATTAGGC, crystal soaked in alternate solvent prior to diffraction 提交 2025-11-03 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | K POTASSIUM ION × 3 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；50mM Magnesium acetate, 1.5M Lithium sulfate, 50mM MES pH 6.5. The crystal was crosslinked with 33mg/mL EDC overnight, and then looped into a solution of 50mM potassium chloride, 4mM calcium chloride, 10% glycerol, and 10mM Tris hydrochloride.
|
分辨率 3.04 Å R-free 0.325 |
| 9Z1D Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence GCTTGATGAG, crystal soaked in alternate solvent prior to diffraction 提交 2025-11-03 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；25mM Magnesium acetate, 800mM Lithium sulfate, 50mM MES pH 6.5. The crystal was crosslinked with 50mg/mL EDC overnight, and then looped into a solution of 10mM HEPES pH 6.5, 50mM sodium chloride, 2.5 mM magnesium chloride, 800mM lithium sulfate, 15mM magnesium acetate, 25mM MES pH 6.5, and 80 micromolar Ultrabithorax homeodomain.
|
分辨率 3.06 Å R-free 0.318 |
| 9Z44 Isoreticular co-crystal 1 with symmetrical expanded duplex (31mer) containing insert sequence CCCGGCCGGA and loaded with C-clamp domain 提交 2025-11-08 | Assembly 1 蛋白–DNA 异源复合物；蛋白 × 2 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；60mM magnesium acetate, 1.8M lithium sulfate, 50mM MES pH 6.5.The crystal was crosslinked with 50 mg/mL EDC overnight, and then looped into a solution of 50mM potassium chloride, 4mM calcium chloride, 10% glycerol, and 10mM Tris hydrochloride for 1 hour. The drop was then supplemented with 90 micromolar C-clamp
|
分辨率 7.20 Å R-free 0.345 |
| 9Z4E Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence CATGAGTCAT and loaded with mutated Bzip region of GCN4 transcription factor 提交 2025-11-10 | Assembly 1 蛋白–DNA 异源复合物；蛋白 × 4 PDB 声明：hexameric（6） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | MG MAGNESIUM ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；50mM magnesium acetate, 1.6M Lithium sulfate, 50mM MES pH 6.5. The crystal was crosslinked with 47.5 mg/mL EDC overnight, and then looped into a solution of 50mM potassium chloride, 4mM calcium chloride, 10% glycerol, and 10mM Tris hydrochloride for 1 hour. The drop was then supplemented with 100 micromolar bZip
|
分辨率 3.63 Å R-free 0.285 |
| 9Z55 Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence CATGAGTCAT 提交 2025-11-11 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 C
1–251（251 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；40mM magnesium acetate, 1.8M lithium sulfate, 50mM MES pH 6.5
|
分辨率 3.94 Å R-free 0.316 |