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9w2s

Cryo-EM structure of FoF1-ATPase monomer state 3 on the bovine heart submitochondrial particles (FoF1-2)

Method: ELECTRON MICROSCOPY D_max: 215.1 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9w2s
deposition_date	2025-07-28
title	Cryo-EM structure of FoF1-ATPase monomer state 3 on the bovine heart submitochondrial particles (FoF1-2)
keywords	ATP synthase, FoF1-ATPase, submitochondrial particles, MOTOR PROTEIN; MOTOR PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	67.66 Å
rg_electron	68.54 Å
i0	4070460000.00
molecular_weight	555810.0 kDa
excluded_volume	703650 Å³
envelope_volume	1107300 Å³
shell_volume	137450 Å³
envelope_diameter	244.8 Å
shell_rg	65.63 Å
envelope_rg	68.36 Å
shape_rg	68.54 Å
total_rg	68.47 Å
total_atoms	39116
n_residues	5147
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	215.1 Å
rg_real	67.91 Å
rg_real_error	1.67 Å
i0_real	4.0690e+09
i0_real_error	8.6130e+07
rg_reciprocal	66.47 Å
i0_reciprocal	4059000000.0000
total_estimate	0.7995
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	65.5 Å
skewness	0.548
kurtosis	-0.387
angular_range	— – 0.1150 Å⁻¹
current_alpha	0.0009
highest_alpha	576300000.0000
n_real_points	24
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.788; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.989; Smooth: 0.038

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4. Crystallography & Experiment

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5. Entities & Polymer Info (18)

Entity 1 polymer

description	ATP synthase F(0) complex subunit 8
formula_weight	7944.5 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	8
atom_count	39116

MPQLDTSTWLTMILSMFLTLFIIFQLKVSKHNFYHNPELTPTKMLKQNTPWETKWTKIYLPLLLPL

Entity 2 polymer

description	ATP synthase F(1) complex subunit alpha, mitochondrial
formula_weight	55331.2 kDa
src_method	nat
entity_count	3

Polymer

type	polypeptide(L)
strand_id	C,A,B

QKTGTAEVSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVGEELLGRVVDALGNAIDGKGPIGSKARRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIGRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGTDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDAFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREVAAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVRGYLDKLEPSKITKFENAFLSHVISQHQALLSKIRTDGKISEESDAKLKEIVTNFLAGFEA

Entity 3 polymer

description	ATP synthase F(1) complex catalytic subunit beta, mitochondrial
formula_weight	51757.8 kDa
ec_number	7.1.2.2
src_method	nat
entity_count	3

Polymer

type	polypeptide(L)
strand_id	F,D,E

AAQASPSPKAGATTGRIVAVIGAVVDVQFDEGLPPILNALEVQGRETRLVLEVAQHLGESTVRTIAMDGTEGLVRGQKVLDSGAPIRIPVGPETLGRIMNVIGEPIDERGPIKTKQFAAIHAEAPEFVEMSVEQEILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFQVAEVFTGHLGKLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEEHS

Entity 4 polymer

description	ATP synthase F(1) complex subunit gamma, mitochondrial
formula_weight	30300.8 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	G

ATLKDITRRLKSIKNIQKITKSMKMVAAAKYARAERELKPARVYGVGSLALYEKADIKTPEDKKKHLIIGVSSDRGLCGAIHSSVAKQMKSEAANLAAAGKEVKIIGVGDKIRSILHRTHSDQFLVTFKEVGRRPPTFGDASVIALELLNSGYEFDEGSIIFNRFRSVISYKTEEKPIFSLDTISSAESMSIYDDIDADVLRNYQEYSLANIIYYSLKESTTSEQSARMTAMDNASKNASEMIDKLTLTFNRTRQAVITKELIEIISGAAALD

Entity 5 polymer

description	ATP synthase F(1) complex subunit delta, mitochondrial
formula_weight	15074.8 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	H

AEAAAAQAPAAGPGQMSFTFASPTQVFFNSANVRQVDVPTQTGAFGILAAHVPTLQVLRPGLVVVHAEDGTTSKYFVSSGSVTVNADSSVQLLAEEAVTLDMLDLGAAKANLEKAQSELLGAADEATRAEIQIRIEANEALVKALE

Entity 6 polymer

description	ATP synthase F(1) complex subunit epsilon, mitochondrial
formula_weight	5662.7 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	I

VAYWRQAGLSYIRYSQICAKAVRDALKTEFKANAMKTSGSTIKIVKVKKE

Entity 7 polymer

description	ATPase inhibitor, mitochondrial
formula_weight	6633.2 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	J

GSESGDNVRSSAGAVRDAGGAFGKREQAEEERYFRARAKEQLAALKKHHENEISHHAKEI

Entity 8 polymer

description	ATP synthase F(0) complex subunit C1, mitochondrial
formula_weight	7653.0 kDa
src_method	nat
entity_count	8

Polymer

type	polypeptide(L)
strand_id	K,L,M,N,O,P,Q,R

DIDTAAKFIGAGAATVGVAGSGAGIGTVFGSLIIGYARNPSLKQQLFSYAILGFALSEAMGLFCLMVAFLILFAM

Entity 9 polymer

description	ATP synthase peripheral stalk subunit OSCP, mitochondrial
formula_weight	20959.8 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	S

FAKLVRPPVQIYGIEGRYATALYSAASKQNKLEQVEKELLRVGQILKEPKMAASLLNPYVKRSVKVKSLSDMTAKEKFSPLTSNLINLLAENGRLTNTPAVISAFSTMMSVHRGEVPCTVTTASALDEATLTELKTVLKSFLSKGQVLKLEVKIDPSIMGGMIVRIGEKYVDMSAKTKIQKLSRAMREIL

Entity 10 polymer

description	ATP synthase F(0) complex subunit a
formula_weight	24801.8 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	a

MNENLFTSFITPVILGLPLVTLIVLFPSLLFPTSNRLVSNRFVTLQQWMLQLVSKQMMSIHNSKGQTWTLMLMSLILFIGSTNLLGLLPHSFTPTTQLSMNLGMAIPLWAGAVITGFRNKTKASLAHFLPQGTPTPLIPMLVIIETISLFIQPMALAVRLTANITAGHLLIHLIGGATLALMSISTTTALITFTILILLTILEFAVAMIQAYVFTLLVSLYLHDNT

Entity 11 polymer

description	ATP synthase peripheral stalk subunit b, mitochondrial
formula_weight	24702.7 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	b

PVPPLPEHGGKVRFGLIPEEFFQFLYPKTGVTGPYVLGTGLILYLLSKEIYVITPETFSAISTIGFLVYIVKKYGASVGEFADKLNEQKIAQLEEVKQASIKQIQDAIDMEKSQQALVQKRHYLFDVQRNNIAMALEVTYRERLHRVYREVKNRLDYHISVQNMMRQKEQEHMINWVEKRVVQSISAQQEKETIAKCIADLKLLSKKAQAQPVM

Entity 12 polymer

description	ATP synthase peripheral stalk subunit d, mitochondrial
formula_weight	18588.3 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	d

AGRKLALKTIDWVAFGEIIPRNQKAVANSLKSWNETLTSRLATLPEKPPAIDWAYYKANVAKAGLVDDFEKKFNALKVPIPEDKYTAQVDAEEKEDVKSCAEFLTQSKTRIQEYEKELEKMRNIIPFDQMTIEDLNEVFPETKLDKKKYPYWPHRPIETL

Entity 13 polymer

description	ATP synthase F(0) complex subunit e, mitochondrial
formula_weight	8205.5 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	e

VPPVQVSPLIKLGRYSALFLGMAYGAKRYNYLKPRAEEERRLAAEEKKKRDEQKRIERELAEAQEDTILK

Entity 14 polymer

description	ATP synthase F(0) complex subunit f, mitochondrial
formula_weight	10184.0 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	f

ASVVPLKEKKLLEVKLGELPSWILMRDFTPSGIAGAFQRGYYRYYNKYVNVKKGSIAGLSMVLAAYVFLNYCRSYKELKHERLRKYH

Entity 15 polymer

description	ATP synthase F(0) complex subunit g, mitochondrial
formula_weight	11298.2 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	g

AEFVRNLAEKAPALVNAAVTYSKPRLATFWYYAKVELVPPTPAEIPTAIQSLKKIINSAKTGSFKQLTVKEALLNGLVATEVWMWFYVGEIIGKRGIIGYDV

Entity 16 polymer

description	ATP synthase peripheral stalk subunit F6, mitochondrial
formula_weight	8971.1 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	h

NKELDPVQKLFVDKIREYRTKRQTSGGPVDAGPEYQQDLDRELFKLKQMYGKADMNTFPNFTFEDPKFEVVEKPQS

Entity 17 polymer

description	ATP synthase F(0) complex subunit j, mitochondrial
formula_weight	6846.1 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	j

MLQSLIKKVWIPMKPYYTQAYQEIWVGTGLMAYIVYKIRSADKRSKALKASSAAPAHGHH

Entity 18 polymer

description	ATP synthase F(0) complex subunit k, mitochondrial
formula_weight	6312.4 kDa
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	k

AGPEADAQFHFTGIKKYFNSYTLTGRMNCVLATYGSIALIVLYFKLRSKKTPAVKAT

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	4.000 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	4.00 Å
resolution_method	—
num_particles	328822

fit	/app/data/saxs_computation/w2/9w2s/9w2s.fit
pddf	/app/data/pr_computation/w2/9w2s/9w2s.pr.out
profile	/app/data/saxs_computation/w2/9w2s/9w2s.dat

Cryo-EM structure of FoF1-ATPase monomer state 3 on the bovine heart submitochondrial particles (FoF1-2)

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (18)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Entity 4 polymer

Polymer

Entity 5 polymer

Polymer

Entity 6 polymer

Polymer

Entity 7 polymer

Polymer

Entity 8 polymer

Polymer

Entity 9 polymer

Polymer

Entity 10 polymer

Polymer

Entity 11 polymer

Polymer

Entity 12 polymer

Polymer

Entity 13 polymer

Polymer

Entity 14 polymer

Polymer

Entity 15 polymer

Polymer

Entity 16 polymer

Polymer

Entity 17 polymer

Polymer

Entity 18 polymer

Polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)