Current Protein Identity：P62979
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 22TU Human 80S ribosome in complex with DHX29 Deposited 2026-01-22 | Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 191 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 28LN XBP1u-stalled RPL4 RNC in complex with NAC Deposited 2026-02-05 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Af
1–156（156 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 2.70 Å |
| 28ZU human 48S PIC with Kozak mRNA and eIF1A W70A mutant Deposited 2026-03-03 | Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric（41） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 3.20 Å |
| 28ZV human 48S PIC with mRNA (non-Kozak) Deposited 2026-03-03 | Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric（41） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 2.90 Å |
| 28ZX human 48S PIC with mRNA (Kozak_1) Deposited 2026-03-03 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric（50） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 2.80 Å |
| 28ZY human 48S PIC with mRNA (Kozak_2) Deposited 2026-03-03 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric（50） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 3.00 Å |
| 3AXC Crystal structure of linear diubiquitin Deposited 2011-04-01 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–76（76 aa）
|
Not recorded | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；289 K；30% PEG 4000, 0.1M Tris-HCl, 0.1M MgCl2, 30% Dioxane, 30% Isopropanol , pH 7.5, VAPOR DIFFUSION, temperature 289K
|
Resolution 2.19 Å R-free 0.278 |
| 3PHW OTU Domain of Crimean Congo Hemorrhagic Fever Virus in complex with Ubiquitin Deposited 2010-11-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–75（75 aa）
Fragment：UNP residues 1-75
|
Not recorded | NEH ETHANAMINE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.27 M sodium phosphate dihydrogen, 1.53 M di-potassium phosphate hydrogen, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.278 |
| 3PHW OTU Domain of Crimean Congo Hemorrhagic Fever Virus in complex with Ubiquitin Deposited 2010-11-04 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–75（75 aa）
Fragment：UNP residues 1-75
|
Not recorded | NEH ETHANAMINE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.27 M sodium phosphate dihydrogen, 1.53 M di-potassium phosphate hydrogen, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.278 |
| 3PHW OTU Domain of Crimean Congo Hemorrhagic Fever Virus in complex with Ubiquitin Deposited 2010-11-04 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
1–75（75 aa）
Fragment：UNP residues 1-75
|
Not recorded | NEH ETHANAMINE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.27 M sodium phosphate dihydrogen, 1.53 M di-potassium phosphate hydrogen, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.278 |
| 3PHW OTU Domain of Crimean Congo Hemorrhagic Fever Virus in complex with Ubiquitin Deposited 2010-11-04 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain H
1–75（75 aa）
Fragment：UNP residues 1-75
|
Not recorded | NEH ETHANAMINE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0.27 M sodium phosphate dihydrogen, 1.53 M di-potassium phosphate hydrogen, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.278 |
| 3TBL Structure of Mono-ubiquitinated PCNA: Implications for DNA Polymerase Switching and Okazaki Fragment Maturation Deposited 2011-08-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain D
1–76（76 aa）
Chain E
1–76（76 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；288 K；0.9 to 1.3 M sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K
|
Resolution 2.90 Å R-free 0.293 |
| 4R62 Structure of Rad6~Ub Deposited 2014-08-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–76（76 aa）
Fragment：Ubiquitin
|
Not recorded | ACT ACETATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；293 K；24% PEG 300, 50 mM sodium acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.28 Å R-free 0.265 |
| 4UG0 STRUCTURE OF THE HUMAN 80S RIBOSOME Deposited 2015-03-20 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 89-meric（89） Review required |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 239 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 4V6X Structure of the human 80S ribosome Deposited 2013-02-27 | Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric（89） Consistent with all polymers |
Chain Af
77–156（80 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 3 seconds using two pieces of filter paper, then plunge into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 5.00 Å |
| 5AJ0 Cryo electron microscopy of actively translating human polysomes (POST state). Deposited 2015-02-19 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Bf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 326 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
20 MM HEPES-KOH, PH 7.5, 100 MM KCL, 1.5 MM MGCL2, 0.5 MM SPERMIDINE, 0.04 MM SPERMINE, 1 MM DTT；pH 7.5；20 MM HEPES-KOH, PH 7.5, 100 MM KCL, 1.5 MM MGCL2, 0.5 MM SPERMIDINE, 0.04 MM SPERMINE, 1 MM DTT
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 93, INSTRUMENT- FEI VITROBOT MARK II, METHOD- BLOT FOR 2-4 SECONDS BEFORE PLUNGING,
|
Resolution 3.50 Å |
| 5FLX Mammalian 40S HCV-IRES complex Deposited 2015-10-28 | Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric（35） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 74 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
20MM TRIS-HCL, 7.5MM MGCL2, 100MM KCL, 0.2MM SPERMIDINE, 2MM DTT；pH 7.6；20MM TRIS-HCL, 7.5MM MGCL2, 100MM KCL, 0.2MM SPERMIDINE, 2MM DTT
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 93, INSTRUMENT- FEI VITROBOT MARK I, METHOD- BLOT FOR 2-4 SECONDS BEFORE PLUNGING,
|
Resolution 3.90 Å |
| 5LKS Structure-function insights reveal the human ribosome as a cancer target for antibiotics Deposited 2016-07-23 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 80-meric（80） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 MG MAGNESIUM ION × 224 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 5T2C CryoEM structure of the human ribosome at 3.6 Angstrom resolution Deposited 2016-08-23 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric（81） Consistent with all polymers |
Chain AH
1–156（156 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 5WVO Crystal structure of DNMT1 RFTS domain in complex with K18/K23 mono-ubiquitylated histone H3 Deposited 2016-12-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–76（76 aa）
Chain B
1–76（76 aa）
|
Mutation：G76C Mutation：G76C | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；100mM Bis-Tris (pH 6.0), 200mM lithium sulfate monohydrate, 20% PEG 10000
|
Resolution 2.00 Å R-free 0.239 |
| 5YDK Crystal structure of RNF168 UDM1 in complex with Lys63-linked diubiquitin, tetrameric form Deposited 2017-09-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–76（76 aa）
Fragment：UNP residues 1-76
Chain D
1–77（77 aa）
Fragment：UNP residues 1-76
|
Mutation：K63R Mutation：D77 | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M Tris pH 7.6
21% PEG3350
|
Resolution 2.50 Å R-free 0.260 |
| 5YDK Crystal structure of RNF168 UDM1 in complex with Lys63-linked diubiquitin, tetrameric form Deposited 2017-09-13 | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain H
1–76（76 aa）
Fragment：UNP residues 1-76
Chain J
1–77（77 aa）
Fragment：UNP residues 1-76
|
Mutation：K63R Mutation：D77 | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M Tris pH 7.6
21% PEG3350
|
Resolution 2.50 Å R-free 0.260 |
| 5YDK Crystal structure of RNF168 UDM1 in complex with Lys63-linked diubiquitin, tetrameric form Deposited 2017-09-13 | Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
1–77（77 aa）
Fragment：UNP residues 1-76
Chain E
1–76（76 aa）
Fragment：UNP residues 1-76
|
Mutation：D77 Mutation：K63R | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M Tris pH 7.6
21% PEG3350
|
Resolution 2.50 Å R-free 0.260 |
| 5YDK Crystal structure of RNF168 UDM1 in complex with Lys63-linked diubiquitin, tetrameric form Deposited 2017-09-13 | Assembly 4 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain I
1–77（77 aa）
Fragment：UNP residues 1-76
Chain K
1–76（76 aa）
Fragment：UNP residues 1-76
|
Mutation：D77 Mutation：K63R | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M Tris pH 7.6
21% PEG3350
|
Resolution 2.50 Å R-free 0.260 |
| 6DC6 Crystal structure of human ubiquitin activating enzyme E1 (Uba1) in complex with ubiquitin Deposited 2018-05-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–76（76 aa）
|
Not recorded | MG MAGNESIUM ION × 2 POP PYROPHOSPHATE 2- × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；285 K；0.1 M MgCl2, 0.2 M Tris HCl pH 8.0, 20% PEG 4000
|
Resolution 3.14 Å R-free 0.250 |
| 6DC6 Crystal structure of human ubiquitin activating enzyme E1 (Uba1) in complex with ubiquitin Deposited 2018-05-04 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–76（76 aa）
|
Not recorded | MG MAGNESIUM ION × 2 POP PYROPHOSPHATE 2- × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；285 K；0.1 M MgCl2, 0.2 M Tris HCl pH 8.0, 20% PEG 4000
|
Resolution 3.14 Å R-free 0.250 |
| 6FEC Human cap-dependent 48S pre-initiation complex Deposited 2017-12-31 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric（50） Consistent with all polymers |
Chain p
82–152（71 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；20 mM Tris HCl,
50 mM KOAc,
2.5 mM MgCl2,
2 mM DTT,
0.25 mM spermidine
0.25 mM GMPPNP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.30 Å |
| 6G18 Cryo-EM structure of a late human pre-40S ribosomal subunit - State C Deposited 2018-03-20 | Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric（35） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6G51 Cryo-EM structure of a late human pre-40S ribosomal subunit - State D Deposited 2018-03-28 | Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 38-meric（38） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 6G53 Cryo-EM structure of a late human pre-40S ribosomal subunit - State E Deposited 2018-03-28 | Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 38-meric（38） Review required |
Chain f
1–156（156 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 6IP5 Cryo-EM structure of the CMV-stalled human 80S ribosome (Structure ii) Deposited 2018-11-02 | Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain 3R
1–156（156 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6IP6 Cryo-EM structure of the CMV-stalled human 80S ribosome with HCV IRES (Structure iii) Deposited 2018-11-02 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain 3R
1–156（156 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 6IP8 Cryo-EM structure of the HCV IRES dependently initiated CMV-stalled 80S ribosome (Structure iv) Deposited 2018-11-02 | Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain 3R
1–156（156 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6J99 Cryo-EM structure of human DOT1L in complex with an H2B-monoubiquitinated nucleosome Deposited 2019-01-22 | Assembly 1 Protein–DNA Heteromer；Protein × 10 PDB declaration: dodecameric（12） Consistent with all polymers |
Chain L
1–76（76 aa）
|
Mutation：G76C | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 6KIU Cryo-EM structure of human MLL1-ubNCP complex (3.2 angstrom) Deposited 2019-07-20 | Assembly 1 Protein–DNA Heteromer；Protein × 13 PDB declaration: pentadecameric（15） Consistent with all polymers |
Chain O
1–76（76 aa）
|
Mutation：G76C | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ZN ZINC ION × 1 LYS LYSINE × 1 GLN GLUTAMINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6KIV Cryo-EM structure of human MLL1-ubNCP complex (4.0 angstrom) Deposited 2019-07-20 | Assembly 1 Protein–DNA Heteromer；Protein × 13 PDB declaration: pentadecameric（15） Consistent with all polymers |
Chain O
1–76（76 aa）
|
Mutation：G76C | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 6KIW Cryo-EM structure of human MLL3-ubNCP complex (4.0 angstrom) Deposited 2019-07-20 | Assembly 1 Protein–DNA Heteromer；Protein × 13 PDB declaration: pentadecameric（15） Consistent with all polymers |
Chain O
1–76（76 aa）
|
Mutation：G76C | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 6OLE Human ribosome nascent chain complex (CDH1-RNC) stalled by a drug-like molecule with AP and PE tRNAs Deposited 2019-04-16 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 31 ZN ZINC ION × 6 MVM N-(3-chloropyridin-2-yl)-N-[(3R)-piperidin-3-yl]-4-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)benzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6OLF Human ribosome nascent chain complex (CDH1-RNC) stalled by a drug-like molecule with AA and PE tRNAs Deposited 2019-04-16 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 31 MVM N-(3-chloropyridin-2-yl)-N-[(3R)-piperidin-3-yl]-4-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)benzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6OLG Human ribosome nascent chain complex stalled by a drug-like small molecule (CDH1_RNC with PP tRNA) Deposited 2019-04-16 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Bf
79–151（73 aa）
|
Not recorded | MG MAGNESIUM ION × 326 ZN ZINC ION × 5 MVM N-(3-chloropyridin-2-yl)-N-[(3R)-piperidin-3-yl]-4-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)benzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 6OLI Structure of human ribosome nascent chain complex selectively stalled by a drug-like small molecule (USO1-RNC) Deposited 2019-04-16 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 31 ZN ZINC ION × 6 MVM N-(3-chloropyridin-2-yl)-N-[(3R)-piperidin-3-yl]-4-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)benzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6OLZ Human ribosome nascent chain complex (PCSK9-RNC) stalled by a drug-like molecule with PP tRNA Deposited 2019-04-17 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain Bf
79–151（73 aa）
|
Not recorded | MG MAGNESIUM ION × 326 ZN ZINC ION × 5 MVM N-(3-chloropyridin-2-yl)-N-[(3R)-piperidin-3-yl]-4-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)benzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6OM0 Human ribosome nascent chain complex (PCSK9-RNC) stalled by a drug-like molecule with AP and PE tRNAs Deposited 2019-04-17 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 31 ZN ZINC ION × 6 MVM N-(3-chloropyridin-2-yl)-N-[(3R)-piperidin-3-yl]-4-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)benzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6OM7 Human ribosome nascent chain complex (PCSK9-RNC) stalled by a drug-like small molecule with AA and PE tRNAs Deposited 2019-04-18 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 31 MVM N-(3-chloropyridin-2-yl)-N-[(3R)-piperidin-3-yl]-4-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)benzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6QZP High-resolution cryo-EM structure of the human 80S ribosome Deposited 2019-03-12 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric（81） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 438 HMT (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine × 1 ZN ZINC ION × 8 HYG HYGROMYCIN B × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 6XA1 Structure of a drug-like compound stalled human translation termination complex Deposited 2020-06-03 | Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Sf
89–151（63 aa）
|
Not recorded | MG MAGNESIUM ION × 190 MVM N-(3-chloropyridin-2-yl)-N-[(3R)-piperidin-3-yl]-4-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)benzamide × 1 ZN ZINC ION × 8 K POTASSIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 6Y0G Structure of human ribosome in classical-PRE state Deposited 2020-02-07 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 72 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6Y57 Structure of human ribosome in hybrid-PRE state Deposited 2020-02-25 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain sh
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 160 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6YBS Structure of a human 48S translational initiation complex - head Deposited 2020-03-17 | Assembly 1 Protein–RNA Heteromer；Protein × 17 PDB declaration: octadecameric（18） Consistent with all polymers |
Chain k
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 29 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6Z6L Cryo-EM structure of human CCDC124 bound to 80S ribosomes Deposited 2020-05-28 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 256 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 6Z6M Cryo-EM structure of human 80S ribosomes bound to EBP1, eEF2 and SERBP1 Deposited 2020-05-28 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 256 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6Z6N Cryo-EM structure of human EBP1-80S ribosomes (focus on EBP1) Deposited 2020-05-28 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 256 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 6ZLW SARS-CoV-2 Nsp1 bound to the human 40S ribosomal subunit Deposited 2020-07-01 | Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain g
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 6ZM7 SARS-CoV-2 Nsp1 bound to the human CCDC124-80S-EBP1 ribosome complex Deposited 2020-07-01 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 256 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 6ZME SARS-CoV-2 Nsp1 bound to the human CCDC124-80S-eERF1 ribosome complex Deposited 2020-07-02 | Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 88-meric（88） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 256 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 6ZMI SARS-CoV-2 Nsp1 bound to the human LYAR-80S ribosome complex Deposited 2020-07-02 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 256 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 6ZMO SARS-CoV-2 Nsp1 bound to the human LYAR-80S-eEF1a ribosome complex Deposited 2020-07-03 | Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric（88） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 256 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6ZMT SARS-CoV-2 Nsp1 bound to a pre-40S-like ribosome complex Deposited 2020-07-03 | Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric（35） Consistent with all polymers |
Chain g
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 6ZMW Structure of a human 48S translational initiation complex Deposited 2020-07-04 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain k
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 89 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6ZON SARS-CoV-2 Nsp1 bound to a human 43S preinitiation ribosome complex - state 1 Deposited 2020-07-07 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 48-meric（48） Consistent with all polymers |
Chain U
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 6ZP4 SARS-CoV-2 Nsp1 bound to a human 43S preinitiation ribosome complex - state 2 Deposited 2020-07-08 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain U
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 6ZUO Human RIO1(kd)-StHA late pre-40S particle, structural state A (pre 18S rRNA cleavage) Deposited 2020-07-23 | Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric（35） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6ZV6 Human RIO1(kd)-StHA late pre-40S particle, structural state B (post 18S rRNA cleavage) Deposited 2020-07-24 | Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric（35） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 3 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 6ZVH EDF1-ribosome complex Deposited 2020-07-24 | Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric（37） Consistent with all polymers |
Chain f
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 30 ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 6ZVJ Structure of a human ABCE1-bound 43S pre-initiation complex - State II Deposited 2020-07-24 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain U
88–149（62 aa）
|
Not recorded | ZN ZINC ION × 3 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6ZXD Cryo-EM structure of a late human pre-40S ribosomal subunit - State F1 Deposited 2020-07-29 | Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 37-meric（37） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6ZXE Cryo-EM structure of a late human pre-40S ribosomal subunit - State F2 Deposited 2020-07-29 | Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 37-meric（37） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 6ZXF Cryo-EM structure of a late human pre-40S ribosomal subunit - State G Deposited 2020-07-29 | Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 37-meric（37） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 3 MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6ZXG Cryo-EM structure of a late human pre-40S ribosomal subunit - State H1 Deposited 2020-07-29 | Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 37-meric（37） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 131 ZN ZINC ION × 3 ASP ASPARTIC ACID × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 6ZXH Cryo-EM structure of a late human pre-40S ribosomal subunit - State H2 Deposited 2020-07-29 | Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | ASP ASPARTIC ACID × 1 MG MAGNESIUM ION × 131 ZN ZINC ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 7A09 Structure of a human ABCE1-bound 43S pre-initiation complex - State III Deposited 2020-08-07 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain U
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 4 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MET METHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 7BWD Structure of Dot1L-H2BK34ub Nucleosome Complex Deposited 2020-04-14 | Assembly 1 Protein–DNA Heteromer；Protein × 11 PDB declaration: tridecameric（13） Consistent with all polymers |
Chain L
1–76（76 aa）
Chain M
1–76（76 aa）
|
Not recorded | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.32 Å |
| 7MQ9 Cryo-EM structure of the human SSU processome, state pre-A1* Deposited 2021-05-05 | Assembly 1 Protein–RNA Heteromer；Protein × 70 PDB declaration: 74-meric（74） Consistent with all polymers |
Chain NT
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 22 ZN ZINC ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.87 Å |
| 7MQA Cryo-EM structure of the human SSU processome, state post-A1 Deposited 2021-05-05 | Assembly 1 Protein–RNA Heteromer；Protein × 69 PDB declaration: 72-meric（72） Consistent with all polymers |
Chain NT
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 64 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 7OOJ Structure of D-Thr53 Ubiquitin Deposited 2021-05-27 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–76（76 aa）
|
Mutation：Gly53D-Thr Non-standard monomer：Yes (specific site not provided by mmCIF) | CD CADMIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M Bis-Tris, pH 7.5, 20 % polyethylene glycol monomethyl ether 2000, 50 mM CdCl2
|
Resolution 2.60 Å R-free 0.269 |
| 7OOJ Structure of D-Thr53 Ubiquitin Deposited 2021-05-27 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–76（76 aa）
|
Mutation：Gly53D-Thr Non-standard monomer：Yes (specific site not provided by mmCIF) | CD CADMIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M Bis-Tris, pH 7.5, 20 % polyethylene glycol monomethyl ether 2000, 50 mM CdCl2
|
Resolution 2.60 Å R-free 0.269 |
| 7QP6 Structure of the human 48S initiation complex in open state (h48S AUG open) Deposited 2022-01-03 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain k
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；20 mM Hepes, pH 7.5,
95 mM KOAc, 3.75 mM Mg(OAc)2, 1 mM ATP, 0.5 mM GTP, 0.25 mM spermidine, 2 mM DTT,
0.4 U/uL RiboLock RNase inhibitor
cryo-EM vitrification conditions
Cryogen ETHANE；Cryo-EM grids were prepared by
floating home-made continuous carbon on 40 ul sample in the wells of teflon block (custom-made). The sample-covered carbon was then adsorbed to an EM grid.
|
Resolution 4.70 Å |
| 7QP7 Structure of the human 48S initiation complex in closed state (h48S AUG closed) Deposited 2022-01-03 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain k
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；20 mM Hepes, pH 7.5,
95 mM KOAc, 3.75 mM Mg(OAc)2, 1 mM ATP, 0.5 mM GTP, 0.25 mM spermidine, 2 mM DTT,
0.4 U/uL RiboLock RNase inhibitor
cryo-EM vitrification conditions
Cryogen ETHANE；Cryo-EM grids were prepared by
floating home-made continuous carbon on 40 ul sample in the wells of teflon block (custom-made). The sample-covered carbon was then adsorbed to an EM grid.
|
Resolution 3.70 Å |
| 7R4X Cryo-EM reconstruction of the human 40S ribosomal subunit - Full map Deposited 2022-02-09 | Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric（35） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | K POTASSIUM ION × 82 MG MAGNESIUM ION × 88 ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.15 Å |
| 7TQL CryoEM structure of the human 40S small ribosomal subunit in complex with translation initiation factors eIF1A and eIF5B. Deposited 2022-01-26 | Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 38-meric（38） Consistent with all polymers |
Chain g
85–149（65 aa）
|
Not recorded | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 7WTT Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-A1 (with CK1) Deposited 2022-02-05 | Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric（34） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 7WTU Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-A1 (without CK1) Deposited 2022-02-05 | Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 33-meric（33） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 7WTV Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-A2 Deposited 2022-02-05 | Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric（35） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 7WTW Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-A3 Deposited 2022-02-05 | Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 37-meric（37） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 7WTX Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-B1 Deposited 2022-02-05 | Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric（34） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 7WTZ Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-B2 Deposited 2022-02-05 | Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric（34） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 7WU0 Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-B3 Deposited 2022-02-05 | Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric（35） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 7XNY High resolution cry-EM structure of the human 80S ribosome from SNORD127+/- Kasumi-1 cells Deposited 2022-04-30 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 79-meric（79） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 421 K POTASSIUM ION × 12 ZN ZINC ION × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 8G5Y mRNA decoding in human is kinetically and structurally distinct from bacteria (IC state) Deposited 2023-02-14 | Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | SPD SPERMIDINE × 13 PUT 1,4-DIAMINOBUTANE × 11 K POTASSIUM ION × 77 MG MAGNESIUM ION × 524 ANM ANISOMYCIN × 1 3H3 4-{(2R,5S,6E)-2-hydroxy-5-methyl-7-[(2R,3S,4E,6Z,10E)-3-methyl-12-oxooxacyclododeca-4,6,10-trien-2-yl]-4-oxooct-6-en-1-yl}piperidine-2,6-dione × 1 ZN ZINC ION × 8 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.29 Å |
| 8G60 mRNA decoding in human is kinetically and structurally distinct from bacteria (CR state) Deposited 2023-02-14 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | SPD SPERMIDINE × 8 PUT 1,4-DIAMINOBUTANE × 10 MG MAGNESIUM ION × 461 ANM ANISOMYCIN × 1 3H3 4-{(2R,5S,6E)-2-hydroxy-5-methyl-7-[(2R,3S,4E,6Z,10E)-3-methyl-12-oxooxacyclododeca-4,6,10-trien-2-yl]-4-oxooct-6-en-1-yl}piperidine-2,6-dione × 1 K POTASSIUM ION × 10 ZN ZINC ION × 7 PHE PHENYLALANINE × 1 MET METHIONINE × 1 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 ZIY plitidepsin × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.54 Å |
| 8G61 mRNA decoding in human is kinetically and structurally distinct from bacteria (AC state) Deposited 2023-02-14 | Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | PUT 1,4-DIAMINOBUTANE × 4 K POTASSIUM ION × 16 MG MAGNESIUM ION × 326 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 5 3H3 4-{(2R,5S,6E)-2-hydroxy-5-methyl-7-[(2R,3S,4E,6Z,10E)-3-methyl-12-oxooxacyclododeca-4,6,10-trien-2-yl]-4-oxooct-6-en-1-yl}piperidine-2,6-dione × 1 ZN ZINC ION × 8 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.94 Å |
| 8G6J mRNA decoding in human is kinetically and structurally distinct from bacteria (GA state 2) Deposited 2023-02-15 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | SPD SPERMIDINE × 10 K POTASSIUM ION × 34 MG MAGNESIUM ION × 337 PUT 1,4-DIAMINOBUTANE × 8 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 HMT (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine × 1 ZN ZINC ION × 8 PHE PHENYLALANINE × 1 MET METHIONINE × 1 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 1 YRB (3R,6R,9S,12S,15S,18S,20R,24aR)-6-[(2S)-butan-2-yl]-3,12-bis[(1R)-1-hydroxy-2-methylpropyl]-8,9,11,17,18-pentamethyl-15-[(2S)-2-methylbutyl]hexadecahydropyrido[1,2-a][1,4,7,10,13,16,19]heptaazacyclohenicosine-1,4,7,10,13,16,19(21H)-heptone × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 8GLP mRNA decoding in human is kinetically and structurally distinct from bacteria (Consensus LSU focused refined structure) Deposited 2023-03-22 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric（81） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | SPD SPERMIDINE × 15 PUT 1,4-DIAMINOBUTANE × 9 K POTASSIUM ION × 152 MG MAGNESIUM ION × 671 ANM ANISOMYCIN × 1 3H3 4-{(2R,5S,6E)-2-hydroxy-5-methyl-7-[(2R,3S,4E,6Z,10E)-3-methyl-12-oxooxacyclododeca-4,6,10-trien-2-yl]-4-oxooct-6-en-1-yl}piperidine-2,6-dione × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 ZN ZINC ION × 8 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.67 Å |
| 8IFD Dibekacin-added human 80S ribosome Deposited 2023-02-17 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 80-meric（80） Consistent with all polymers |
Chain 3R
1–156（156 aa）
|
Not recorded | 84D Dibekacin × 24 MG MAGNESIUM ION × 397 ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.59 Å |
| 8IFE Arbekacin-added human 80S ribosome Deposited 2023-02-17 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 80-meric（80） Consistent with all polymers |
Chain 3R
1–156（156 aa）
|
Not recorded | 84G Arbekacin × 23 MG MAGNESIUM ION × 410 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.57 Å |
| 8IVB K113-Ubiquitinated BAK Deposited 2023-03-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–76（76 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.8；310 K；Ionic strength (raw mmCIF value) 100；Pressure 1
NMR sample composition
20 mM sodium phosphate, 0.1 M sodium chloride, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 8K2C Cryo-EM structure of the human 80S ribosome with Tigecycline Deposited 2023-07-12 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 259 T1C TIGECYCLINE × 9 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 8OZ0 Structure of a human 48S translation initiation complex with eIF4F and eIF4A Deposited 2023-05-06 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain p
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 89 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8PJ1 Structure of human 48S translation initiation complex in open codon scanning state (48S-1) Deposited 2023-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain k
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 90 ZN ZINC ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Manual blotting & plunge-freezing
|
Resolution 3.40 Å |
| 8PJ2 Structure of human 48S translation initiation complex in AUG recognition state after eIF5-induced GTP hydrolysis by eIF2 (48S-2) Deposited 2023-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 46 PDB declaration: 49-meric（49） Consistent with all polymers |
Chain k
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 87 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Manual blotting & plunge-freezing
|
Resolution 3.40 Å |
| 8PJ3 Structure of human 48S translation initiation complex upon transfer of initiator tRNA to eIF5B (48S-3) Deposited 2023-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain k
1–156（156 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NA SODIUM ION × 1 MG MAGNESIUM ION × 89 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Manual blotting & plunge-freezing
|
Resolution 3.70 Å |
| 8PJ4 Structure of human 48S translation initiation complex after eIF5 release (48S-4) Deposited 2023-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain k
1–156（156 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NA SODIUM ION × 1 MG MAGNESIUM ION × 89 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Manual blotting & plunge-freezing
|
Resolution 3.20 Å |
| 8PJ5 Structure of human 48S translation initiation complex after eIF2 release prior 60S subunit joining (48S-5) Deposited 2023-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain k
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 91 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Manual blotting & plunge-freezing
|
Resolution 2.90 Å |
| 8PJ6 Structure of human 48S translation initiation complex with initiator tRNA, eIF1A and eIF3 (off-pathway) Deposited 2023-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric（50） Consistent with all polymers |
Chain k
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 87 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Manual blotting & plunge-freezing
|
Resolution 2.90 Å |
| 8PPK Bat-Hp-CoV Nsp1 and eIF1 bound to the human 40S small ribosomal subunit Deposited 2023-07-07 | Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 37-meric（37） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | UNX UNKNOWN LIGAND × 117 MG MAGNESIUM ION × 111 ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.98 Å |
| 8PPL MERS-CoV Nsp1 bound to the human 43S pre-initiation complex Deposited 2023-07-07 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain Af
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 116 MET METHIONINE × 1 UNX UNKNOWN LIGAND × 129 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.65 Å |
| 8QOI Structure of the human 80S ribosome at 1.9 A resolution - the molecular role of chemical modifications and ions in RNA Deposited 2023-09-29 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 79-meric（79） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 292 K POTASSIUM ION × 108 SPD SPERMIDINE × 2 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6；Titan Krios sample is prepared with Sodium cacodylate buffer.
CryoARM 300 sample is prepared with Hepes buffer.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.90 Å |
| 8T4S MERS-CoV Nsp1 protein bound to the Human 40S Ribosomal subunit Deposited 2023-06-09 | Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 97 ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 8UKB In situ HHT and CHX treated human hibernating state without E-tRNA 80S ribosome Deposited 2023-10-12 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 255 HMT (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine × 1 WV3 4-{(2R)-2-[(1R,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.05 Å |
| 8X7I Cryo-EM structures of RNF168/UbcH5c-Ub in complex with H2AK13Ub nucleosomes determined by intein-based E2-Ub-NCP conjugation strategy Deposited 2023-11-24 | Assembly 1 Protein–DNA Heteromer；Protein × 11 PDB declaration: tridecameric（13） Consistent with all polymers |
Chain M
1–75（75 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.27 Å |
| 8X7J Cryo-EM structures of RNF168/UbcH5c-Ub/nucleosomes complex determined by activity-based chemical trapping strategy Deposited 2023-11-24 | Assembly 1 Protein–DNA Heteromer；Protein × 11 PDB declaration: tridecameric（13） Consistent with all polymers |
Chain M
1–75（75 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.39 Å |
| 8XP2 Cryo-EM structure of the human 40S ribosome with LARP1 Deposited 2024-01-02 | Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8XP3 Cryo-EM structure of the human 40S ribosome with LARP1 and LRRC47 Deposited 2024-01-02 | Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 37-meric（37） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8XSX Cryo-EM structure of the human 80S ribosome with Tigecycline, E-tRNA, SERBP1 and eEF2 Deposited 2024-01-10 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 259 T1C TIGECYCLINE × 7 ZN ZINC ION × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 8XSY Cryo-EM structure of the human 80S ribosome with Tigecycline, e-tRNA and CCDC124 (40S head Swivelled) Deposited 2024-01-10 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 255 T1C TIGECYCLINE × 8 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8XSZ Cryo-EM structure of the human 80S ribosome with Tigecycline, E-tRNA and P-tRNA Deposited 2024-01-10 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 248 T1C TIGECYCLINE × 5 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8XXL Cryo-EM structure of the human 40S ribosome with PDCD4 Deposited 2024-01-18 | Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 22 ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8XXM Cryo-EM structure of the human 40S ribosome with PDCD4 and eIF3G Deposited 2024-01-18 | Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 37-meric（37） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 23 ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8XXN Cryo-EM structure of the human 43S ribosome with PDCD4 Deposited 2024-01-18 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 50-meric（50） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 23 ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8YOO Cryo-EM structure of the human 80S ribosome with 100 um Tigecycline Deposited 2024-03-13 | Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 81-meric（81） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 257 T1C TIGECYCLINE × 6 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.00 Å |
| 8YOP Cryo-EM structure of the human 80S ribosome with 4 um Tigecycline Deposited 2024-03-13 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 255 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å |
| 8ZDB Cryo-EM structure of the human ubiquitylated 40S ribosome with RIOK3 Deposited 2024-05-01 | Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 38-meric（38） Consistent with all polymers |
Chain f
1–156（156 aa）
Chain i
1–156（156 aa）
Chain j
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8ZDC Cryo-EM structure of the human ubiquitylated pre-40S ribosome with RIOK3 Deposited 2024-05-01 | Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 38-meric（38） Consistent with all polymers |
Chain i
1–76（76 aa）
Chain j
1–76（76 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8ZDD Cryo-EM structure of the human ubiquitylated pre-40S ribosome with RIOK3 (without NOB1) Deposited 2024-05-01 | Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 37-meric（37） Consistent with all polymers |
Chain i
1–76（76 aa）
Chain j
1–76（76 aa）
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 9B0P In situ human Hibernating class1 80S ribosome Deposited 2024-03-12 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 255 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.82 Å |
| 9B0Q In situ human top-back di-ribosome structure (Composite map) Deposited 2024-03-12 | Assembly 1 Protein–RNA Heteromer；Protein × 160 PDB declaration: 172-meric（172） Consistent with all polymers |
Chain Sf
85–151（67 aa）
Chain sf
85–151（67 aa）
|
Not recorded | ZN ZINC ION × 12 MG MAGNESIUM ION × 510 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9B0S In situ human top-top di-ribosome structure (Composite map) Deposited 2024-03-12 | Assembly 1 Protein–RNA Heteromer；Protein × 158 PDB declaration: 171-meric（171） Consistent with all polymers |
Chain Sf
85–151（67 aa）
Chain sf
85–151（67 aa）
|
Not recorded | ZN ZINC ION × 12 MG MAGNESIUM ION × 510 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 9BKD The structure of human Pdcd4 bound to the 40S small ribosomal subunit Deposited 2024-04-27 | Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain k
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 3 K POTASSIUM ION × 19 MG MAGNESIUM ION × 88 SPD SPERMIDINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9BLN The structure of human Pdcd4 bound to the 40S-eIF4A-eIF3-eIF1 complex Deposited 2024-04-30 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 50-meric（50） Consistent with all polymers |
Chain k
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 88 K POTASSIUM ION × 19 SPD SPERMIDINE × 1 ZN ZINC ION × 3 ACY ACETIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 9C3H Structure of the CNOT3-bound human 80S ribosome with tRNA-ARG in the P-site. Deposited 2024-06-01 | Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Sz
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 326 K POTASSIUM ION × 94 B3P 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.00 Å |
| 9ED0 Human LARP1 bound to the 40S small ribosomal subunit Deposited 2024-11-15 | Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain f
1–156（156 aa）
|
Not recorded | K POTASSIUM ION × 71 MG MAGNESIUM ION × 92 ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9FQZ CRYO-EM STRUCTURE OF HCT15 POLYSOMES BOUND TO EEF2, EBP1, AND SERBP1 Deposited 2024-06-17 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 271 K POTASSIUM ION × 104 ZN ZINC ION × 8 PUT 1,4-DIAMINOBUTANE × 7 HYG HYGROMYCIN B × 1 SPD SPERMIDINE × 8 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ANM ANISOMYCIN × 1 NA SODIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.85 Å |
| 9G8M human 80S ribosome bound by a SKI2-exosome complex Deposited 2024-07-23 | Assembly 1 Insufficient information Heteromer；Protein × 87 PDB declaration: 92-meric（92） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 232 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.30 Å |
| 9G8O human 40S ribosome bound by a SKI238-exosome complex Deposited 2024-07-23 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.40 Å |
| 9I14 CRYO-EM STRUCTURE OF HCT15 POLYSOMES IN HYBRID-PRE STATE Deposited 2025-01-15 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | K POTASSIUM ION × 40 MG MAGNESIUM ION × 267 NA SODIUM ION × 23 SPD SPERMIDINE × 1 PUT 1,4-DIAMINOBUTANE × 1 ZN ZINC ION × 7 HYG HYGROMYCIN B × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.34 Å |
| 9I2D NMT1-NAC bound human RNC with 10 amino acid ARF1-linker Deposited 2025-01-20 | Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric（88） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 191 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.19 Å |
| 9I2E NMT1-NAC bound human ribosome (combined translational states) Deposited 2025-01-20 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 256 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.95 Å |
| 9IJU Sertraline enhances the deubiquitinase activity of USP7 by binding to its switching loop region Deposited 2024-06-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–75（75 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M Bis-tris pH 6.5, 0.2 M MgCl2, 25% PEG3350
|
Resolution 2.46 Å R-free 0.270 |
| 9IJU Sertraline enhances the deubiquitinase activity of USP7 by binding to its switching loop region Deposited 2024-06-25 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–75（75 aa）
|
Not recorded | SRE (1S,4S)-4-(3,4-dichlorophenyl)-N-methyl-1,2,3,4-tetrahydronaphthalen-1-amine × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M Bis-tris pH 6.5, 0.2 M MgCl2, 25% PEG3350
|
Resolution 2.46 Å R-free 0.270 |
| 9IJU Sertraline enhances the deubiquitinase activity of USP7 by binding to its switching loop region Deposited 2024-06-25 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
1–75（75 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M Bis-tris pH 6.5, 0.2 M MgCl2, 25% PEG3350
|
Resolution 2.46 Å R-free 0.270 |
| 9IJU Sertraline enhances the deubiquitinase activity of USP7 by binding to its switching loop region Deposited 2024-06-25 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain H
1–75（75 aa）
|
Not recorded | SRE (1S,4S)-4-(3,4-dichlorophenyl)-N-methyl-1,2,3,4-tetrahydronaphthalen-1-amine × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M Bis-tris pH 6.5, 0.2 M MgCl2, 25% PEG3350
|
Resolution 2.46 Å R-free 0.270 |
| 9IML Sertraline enhances the deubiquitinase activity of USP7 by binding to its switching loop region Deposited 2024-07-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–75（75 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M Bis-tris pH 6.5, 0.2 M MgCl2, 25% PEG3350
|
Resolution 2.78 Å R-free 0.280 |
| 9IML Sertraline enhances the deubiquitinase activity of USP7 by binding to its switching loop region Deposited 2024-07-03 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–75（75 aa）
|
Not recorded | XB7 1-[(4-fluorophenyl)methyl]-N-{1-[2-(4-methoxyphenyl)ethyl]piperidin-4-yl}-1H-benzimidazol-2-amine × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M Bis-tris pH 6.5, 0.2 M MgCl2, 25% PEG3350
|
Resolution 2.78 Å R-free 0.280 |
| 9IML Sertraline enhances the deubiquitinase activity of USP7 by binding to its switching loop region Deposited 2024-07-03 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
1–75（75 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M Bis-tris pH 6.5, 0.2 M MgCl2, 25% PEG3350
|
Resolution 2.78 Å R-free 0.280 |
| 9IML Sertraline enhances the deubiquitinase activity of USP7 by binding to its switching loop region Deposited 2024-07-03 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain H
1–75（75 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.1 M Bis-tris pH 6.5, 0.2 M MgCl2, 25% PEG3350
|
Resolution 2.78 Å R-free 0.280 |
| 9IPU cryo-EM structure of the RNF168(1-193)/UbcH5c-Ub ubiquitylation module bound to H1.0-K63-Ub3 modified chromatosome Deposited 2024-07-11 | Assembly 1 Protein–DNA Heteromer；Protein × 12 PDB declaration: tetradecameric（14） Consistent with all polymers |
Chain M
1–75（75 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 9K4J Structure of substrate-engaged human 26S proteasome RP-CP subcomplex in state EA1.0 Deposited 2024-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 50 PDB declaration: 50-meric（50） Consistent with protein count |
Chain u
1–76（76 aa）
Chain w
1–76（76 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9K4K Structure of substrate-engaged human 26S proteasome RP-CP subcomplex in state EA1.1 Deposited 2024-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 51 PDB declaration: 51-meric（51） Consistent with protein count |
Chain u
1–76（76 aa）
Chain v
1–76（76 aa）
Chain w
1–76（76 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 9K4L Structure of substrate-engaged 26S proteasome RP-CP subcomplex in state EA1.2 Deposited 2024-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric（48） Consistent with protein count |
Chain x
1–76（76 aa）
Chain y
1–76（76 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.50 Å |
| 9K4M Structure of substrate-engaged human 26S proteasome RP-CP subcomplex in state EA2.1 Deposited 2024-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric（48） Consistent with protein count |
Chain u
1–76（76 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9K4O Structure of substrate-engaged human 26S proteasome RP-CP subcomplex in state EA2.2 Deposited 2024-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 50 PDB declaration: 50-meric（50） Consistent with protein count |
Chain u
1–76（76 aa）
Chain x
1–76（76 aa）
Chain y
1–76（76 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.00 Å |
| 9K4P Structure of substrate-engaged human 26S proteasome RP-CP subcomplex in state EA2.3 Deposited 2024-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric（49） Consistent with protein count |
Chain u
1–76（76 aa）
Chain z
1–76（76 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å |
| 9K4R Structure of substrate-engaged human 26S proteasome RP-CP subcomplex in state EB.1 Deposited 2024-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric（48） Consistent with protein count |
Chain u
1–76（76 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9K4S Structure of substrate-engaged 26S proteasome RP-CP subcomplex in state EB.2 Deposited 2024-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 50 PDB declaration: 50-meric（50） Consistent with protein count |
Chain u
1–76（76 aa）
Chain x
1–76（76 aa）
Chain y
1–76（76 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.70 Å |
| 9K4T Structure of substrate-engaged human 26S proteasome RP-CP subcomplex in state EB.3 Deposited 2024-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric（49） Consistent with protein count |
Chain u
1–76（76 aa）
Chain z
1–76（76 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.10 Å |
| 9K4U Structure of substrate-engaged human 26S proteasome RP-CP subcomplex in state EC1 Deposited 2024-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric（48） Consistent with protein count |
Chain u
1–76（76 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 3 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9K54 Structure of substrate-engaged human 26S proteasome RP-CP subcomplex in state ED2.2 Deposited 2024-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 51 PDB declaration: 51-meric（51） Consistent with protein count |
Chain w
1–76（76 aa）
Chain x
1–76（76 aa）
Chain y
1–76（76 aa）
Chain z
1–76（76 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 9K55 Structure of substrate-engaged human 26S proteasome RP-CP subcomplex in state ED2.3 Deposited 2024-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 50 PDB declaration: 50-meric（50） Consistent with protein count |
Chain w
1–76（76 aa）
Chain x
1–76（76 aa）
Chain z
1–76（76 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.00 Å |
| 9K56 Structure of substrate-engaged single-cap human proteasome in state EA1 Deposited 2024-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain w
1–76（76 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 9K57 Structure of substrate-engaged single-cap human proteasome in state EA2 Deposited 2024-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric（48） Consistent with protein count |
Chain u
1–76（76 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 9K58 Structure of substrate-engaged single-cap human proteasome in state EB Deposited 2024-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric（48） Consistent with protein count |
Chain u
1–76（76 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 9K59 Structure of substrate-engaged single-cap human proteasome in state EC1 Deposited 2024-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric（48） Consistent with protein count |
Chain u
1–76（76 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 3 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 9K5E Structure of substrate-engaged double-cap human proteasome in state EA1-EA1 Deposited 2024-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 68 PDB declaration: 68-meric（68） Consistent with protein count |
Chain Au
1–76（76 aa）
Chain Aw
1–76（76 aa）
Chain Bu
1–76（76 aa）
Chain Bw
1–76（76 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 8 MG MAGNESIUM ION × 10 ADP ADENOSINE-5'-DIPHOSPHATE × 4 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 9KEN cryo-EM structure of TRIP12 in complex with K29/48 branched-triUb Deposited 2024-11-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
1–76（76 aa）
Chain D
1–76（76 aa）
|
Mutation：K29C | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.63 Å |
| 9KHS Cryo-EM structure of Ufd2/Ubc4-Ub in complex with K29-linked diUb (monomeric conformation) Deposited 2024-11-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain C
1–76（76 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.31 Å |
| 9KHT cryo-EM structure of Ufd2/Ubc4-Ub in complex with K29-linked triUb (dimeric conformation) Deposited 2024-11-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric（12） Consistent with protein count |
Chain C
1–76（76 aa）
Chain E
1–76（76 aa）
Chain F
1–76（76 aa）
Chain c
1–76（76 aa）
Chain e
1–76（76 aa）
Chain f
1–76（76 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.85 Å |
| 9KKF Structure of the human 40S ribosome complexed with HCV IRES and eIF3 Deposited 2024-11-13 | Assembly 1 Protein–RNA Heteromer；Protein × 43 PDB declaration: 45-meric（45） Consistent with all polymers |
Chain sh
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9KMT Bat SARSr-CoV RaTG15 Nsp1 bound to the Human 40S Ribosomal subunit-State1 Deposited 2024-11-18 | Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain f
78–151（74 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.81 Å |
| 9KMU Bat SARSr-CoV RaTG15 Nsp1 bound to the Human 40S Ribosomal subunit-State2 Deposited 2024-11-18 | Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain f
78–151（74 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.87 Å |
| 9KMV SARSr-MpCoV-GX Nsp1 bound to the Human 40S Ribosomal subunit-State1 Deposited 2024-11-18 | Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain f
78–151（74 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.65 Å |
| 9KMW SARSr-MpCoV-GX Nsp1 bound to the Human 40S Ribosomal subunit-State2 Deposited 2024-11-18 | Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain f
78–151（74 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.73 Å |
| 9KMX Bat MERSr-CoV NeoCoV Nsp1 bound to the Human 40S Ribosomal subunit-State1 Deposited 2024-11-18 | Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain f
78–151（74 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.89 Å |
| 9KMY Bat MERSr-CoV NeoCoV Nsp1 bound to the Human 40S Ribosomal subunit-State2 Deposited 2024-11-18 | Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain f
78–151（74 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9KMZ Bat MERSr-CoV NL140422 Nsp1 bound to the Human 40S Ribosomal subunit-State1 Deposited 2024-11-18 | Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain f
78–151（74 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.78 Å |
| 9KN0 Bat MERSr-CoV NL140422 Nsp1 bound to the Human 40S Ribosomal subunit-State2 Deposited 2024-11-18 | Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain f
78–151（74 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.65 Å |
| 9KN5 Structure of the human 40S ribosome complexed with HCV IRES, eIF1A and eIF3 Deposited 2024-11-18 | Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 46-meric（46） Consistent with all polymers |
Chain sh
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9KN6 Structure of the HCV IRES-dependent pre-48S translation initiation complex with eIF1A, eIF5B, and eIF3 Deposited 2024-11-18 | Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 48-meric（48） Consistent with all polymers |
Chain sh
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9KRP Structure of the HCV IRES-dependent 48S translation initiation complex with eIF5B and eIF3 Deposited 2024-11-28 | Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric（47） Consistent with all polymers |
Chain sh
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 9 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9KZU Cryo-EM structure of the HCV IRES-dependently initiated CMV-stalled 80S ribosome (non-rotated state) in complexed with eIF3 Deposited 2024-12-11 | Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 92-meric（92） Consistent with all polymers |
Chain sh
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 81 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9KZX Cryo-EM structure of the HCV IRES-dependently initiated CMV-stalled 80S ribosome (rotated state) in complexed with eIF3 Deposited 2024-12-11 | Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 93-meric（93） Consistent with all polymers |
Chain sh
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 66 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9L3L Crystal structure of the C lobe of E6AP in complex with K48-linked diUb Deposited 2024-12-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–76（76 aa）
Chain C
1–73（73 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；0.2 M Sodium citrate tribasic dihydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% v/v Polyethylene glycol 400
|
Resolution 2.59 Å R-free 0.269 |
| 9M7O Cryo-EM structure of Ufd2/Ubc4-ub complex with K29triUb(monomeric conformation) Deposited 2025-03-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain C
1–76（76 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.14 Å |
| 9O3V Human 80S ribosome stalled on MYC nascent chain Deposited 2025-04-07 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 352 K POTASSIUM ION × 165 SPD SPERMIDINE × 14 PUT 1,4-DIAMINOBUTANE × 7 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；20mM Tris, 100mM KOAc, 7.5mM MgOAc, 0.25mM Spermidine, 2mM DTT, 0.05% w/v b-OG
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å |
| 9O3W Human 80S ribosome bound to IDB-001 stalled on MYC nascent chain Deposited 2025-04-07 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | K POTASSIUM ION × 170 PUT 1,4-DIAMINOBUTANE × 7 SPD SPERMIDINE × 14 A1B75 (2R,3S,4S)-4-hydroxy-2-[(4-methoxyphenyl)methyl]pyrrolidin-3-yl {2-[(2S)-pyrrolidin-2-yl]ethyl}carbamate × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 MG MAGNESIUM ION × 371 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；20mM Tris, 100mM KOAc, 7.5mM MgOAc, 0.25mM Spermidine, 2mM DTT, 0.05% w/v b-OG, 50uM IDB-001
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.90 Å |
| 9O3Y Human 80S ribosome bound to IDB-002 stalled on FPAK-containing nascent chain Deposited 2025-04-07 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | K POTASSIUM ION × 168 SPD SPERMIDINE × 14 PUT 1,4-DIAMINOBUTANE × 7 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 MG MAGNESIUM ION × 332 A1B76 (2R,3S,4S)-4-hydroxy-2-[(4-methoxyphenyl)methyl]pyrrolidin-3-yl [(3-fluorophenyl)methyl]carbamate × 1 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；20mM Tris, 100mM KOAc, 7.5mM MgOAc, 0.25mM Spermidine, 2mM DTT, 0.05% w/v b-OG, 50uM IDB-001
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å |
| 9P6Z In situ human P-Z state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.97 Å |
| 9P72 In situ human P-E state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9P73 In situ human eEF2-A/P-P/E state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 218 ZN ZINC ION × 6 SPM SPERMINE × 7 SPD SPERMIDINE × 10 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.66 Å |
| 9P76 In situ human eEF1A-A/T-P state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.83 Å |
| 9P78 In situ human Hibernating rotate 3 with E-site tRNA state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 218 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9P79 In situ human Hibernating rotate3 with Z site tRNA state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 218 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9P7A In situ human hibernating class2 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 218 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.81 Å |
| 9P7C In situ human Hibernating class3 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 218 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.78 Å |
| 9P7D In situ human Hibernating class4 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 218 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.57 Å |
| 9P7E In situ human Hibernating class5 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 218 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.59 Å |
| 9P7F In situ HHT and CHX treated A-P state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 HMT (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.56 Å |
| 9P7G In situ HHT and CHX treated A-P-Z state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 219 ZN ZINC ION × 6 SPM SPERMINE × 7 SPD SPERMIDINE × 10 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 HMT (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.54 Å |
| 9P7H In situ HHT and CHX treated human eEF1A-A/T-P state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 HMT (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine × 1 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.68 Å |
| 9P7I In situ HHT and CHX treated human eEF1A-A/T-P-Z state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 HMT (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine × 1 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.69 Å |
| 9P7J In situ HHT and CHX treated human P state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 HMT (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine × 1 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.91 Å |
| 9P7K In situ human post eEF1A-A/T-P-E state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9P7L In situ human Post-eEF1A-AT-P state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.92 Å |
| 9P7N In situ human Post-eEF1A-A/T-P-Z state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.83 Å |
| 9P7O In situ human AT-P-Z state ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.65 Å |
| 9P7W In situ human A-P state 80S ribosome Deposited 2025-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 219 ZN ZINC ION × 6 SPM SPERMINE × 7 SPD SPERMIDINE × 10 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.93 Å |
| 9P7X In situ human A-P-Z state 80S ribosome Deposited 2025-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.83 Å |
| 9P7Y In situ human A-P-E state 80S ribosome Deposited 2025-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 219 ZN ZINC ION × 6 SPM SPERMINE × 7 SPD SPERMIDINE × 10 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.75 Å |
| 9P8B In situ human eEF1A-A/T-P-E state 80S ribosome Deposited 2025-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.48 Å |
| 9P8C In situ human Hibernating class1 (rotate3) without E tRNA state 80S ribosome Deposited 2025-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 218 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.11 Å |
| 9P8H In situ human A/P-P/E state 80S ribosome Deposited 2025-06-23 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 218 ZN ZINC ION × 6 SPM SPERMINE × 7 SPD SPERMIDINE × 10 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.98 Å |
| 9P8I In situ HHT and CHX treated human P-Z state 80S ribosome Deposited 2025-06-23 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 HMT (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine × 1 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.89 Å |
| 9P9H In situ human unrotated hibernating with CCDC124 state 80S ribosome Deposited 2025-06-24 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.84 Å |
| 9P9I In situ human unrotated hibernating without CCDC124 state 80S ribosome Deposited 2025-06-24 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.77 Å |
| 9PA7 In situ human 80S consensus ribosome with EBP1 Deposited 2025-06-25 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric（87） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 225 SPM SPERMINE × 8 SPD SPERMIDINE × 12 K POTASSIUM ION × 75 ZN ZINC ION × 7 PUT 1,4-DIAMINOBUTANE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.67 Å |
| 9PBE In situ human 80S ribosome (composite map) Deposited 2025-06-26 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 225 SPM SPERMINE × 8 SPD SPERMIDINE × 12 K POTASSIUM ION × 75 ZN ZINC ION × 7 PUT 1,4-DIAMINOBUTANE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.19 Å |
| 9PKG In situ human P state 80S ribosome Deposited 2025-07-14 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Sf
85–151（67 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.26 Å |
| 9QLO NMT1-NAC bound human RNC with full length ARF1 - State 1 Deposited 2025-03-21 | Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric（88） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 191 G3D GUANOSINE-3'-MONOPHOSPHATE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.47 Å |
| 9QLP NMT1-NAC bound human RNC with full length ARF1 - State 2 Deposited 2025-03-21 | Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric（88） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 191 G3D GUANOSINE-3'-MONOPHOSPHATE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.75 Å |
| 9QLQ NMT1-NAC bound human RNC with full length ARF1 - alternative State Deposited 2025-03-21 | Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric（88） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 191 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.57 Å |
| 9RPV Structure of the ZAK-bound human disome Deposited 2025-06-25 | Assembly 1 Protein–RNA Heteromer；Protein × 154 PDB declaration: 168-meric（168） Consistent with all polymers |
Chain Rf
1–156（156 aa）
Chain sh
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 830 ZN ZINC ION × 7 ANM ANISOMYCIN × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.35 Å |
| 9RU7 WT-HEK 80S ribosome bound to Kozak mRNA (WT-Kozak) Deposited 2025-07-03 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | K POTASSIUM ION × 17 MG MAGNESIUM ION × 74 C CYTIDINE-5'-MONOPHOSPHATE × 2 NA SODIUM ION × 7 ZN ZINC ION × 5 HYG HYGROMYCIN B × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9RU9 RPS26dC HEK mutant 80S ribosome bound to Kozak mRNA (RPS26dC-Kozak) Deposited 2025-07-03 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 2.97 Å |
| 9S3B NMT1-NAC bound human RNC with 58 amino acid ARF1-linker - State 1 Deposited 2025-07-24 | Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric（88） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 191 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.38 Å |
| 9S3C NMT1-NAC bound human RNC with 58 amino acid ARF1-linker - State 2 Deposited 2025-07-24 | Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric（88） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 191 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.42 Å |
| 9S3D NAC bound human RNC with 58 amino acid ARF1-linker Deposited 2025-07-24 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric（87） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | MG MAGNESIUM ION × 191 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.32 Å |
| 9SPF CRYO-EM STRUCTURE OF HUMAN 80S RIBOSOME WITH A/P/E-SITE TRNA AND MRNA CONTAINING N1-METHYLPSEUDOURIDINE Deposited 2025-09-17 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | K POTASSIUM ION × 151 MG MAGNESIUM ION × 225 NA SODIUM ION × 35 SPD SPERMIDINE × 19 PUT 1,4-DIAMINOBUTANE × 9 ZN ZINC ION × 7 HYG HYGROMYCIN B × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 9SPI CRYO-EM STRUCTURE OF HUMAN 80S RIBOSOME WITH A/P/E-SITE TRNA AND MRNA CONTAINING URIDINE Deposited 2025-09-17 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Sf
1–156（156 aa）
|
Not recorded | PUT 1,4-DIAMINOBUTANE × 8 SPD SPERMIDINE × 18 MG MAGNESIUM ION × 195 K POTASSIUM ION × 75 ZN ZINC ION × 7 HYG HYGROMYCIN B × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 9SYR Human quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatD Deposited 2025-10-13 | Assembly 1 Insufficient information Heteromer；Protein × 80 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Sr
1–156（156 aa）
|
Not recorded | ZN ZINC ION × 10 COA COENZYME A × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.55 Å |
| 9U5U The 1:1 cryo-EM structure of BAP1/ASXL1-K351Ub in complex with H2AK119Ub nucleosome Deposited 2025-03-21 | Assembly 1 Protein–DNA Heteromer；Protein × 12 PDB declaration: tetradecameric（14） Consistent with all polymers |
Chain M
1–76（76 aa）
Chain O
1–76（76 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.12 Å |
| 9UUM Cryo-EM structure of mezigdomide-organized CRL4-DDB1-CRBN-IKZF3(ZF2-ZF3)-UbcH5a-Ub ubiquitylation assembly Deposited 2025-05-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain U
1–76（76 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 3.41 Å |
| 9UV6 Encounter complex structure of E2N and Ubiquitin Deposited 2025-05-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–76（76 aa）
|
Not recorded | A1EQF (5~{S},16~{S})-16-[2-[2,5-bis(oxidanylidene)pyrrolidin-1-yl]ethylamino]-2,11,12,17-tetraoxa-5,8-diaza-1$l^{4}-manganatricyclo[6.3.3.3^{1,5}]heptadecane-3,10,13-trione × 1 | SOLUTION NMR |
NMR measurement conditions
pH 7.5；298 K；Ionic strength (raw mmCIF value) 0.02；Pressure 1
NMR sample composition
0.6 mM [U-15N] Ubi, 0.2 mM E2N, 20 mM HEPES, 90 % H2O, 10 % [U-2H] D2O, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 9V0A Cryo-EM structure of pomalidomide-organized CRL4-DDB1-CRBN-IKZF3(ZF2-ZF3)-UbcH5a-Ub ubiquitylation assembly Deposited 2025-05-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain U
1–76（76 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 3.69 Å |
| 9V0B Cryo-EM structure of avadomide-organized CRL4-DDB1-CRBN-IKZF3(ZF2-ZF3)-UbcH5a-Ub ubiquitylation assembly Deposited 2025-05-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain U
1–76（76 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 3.54 Å |
| 9V0F Cryo-EM structure of cemsidomide-organized CRL4-DDB1-CRBN-IKZF3(ZF2-ZF3)-UbcH5a-Ub ubiquitylation assembly Deposited 2025-05-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain U
1–76（76 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 3.71 Å |
| 9V9Q Cryo-EM structure of the cPRC1-UbcH5c E3-E2 complex bound to the H2BK120ub-modified nucleosome Deposited 2025-06-02 | Assembly 1 Protein–DNA Heteromer；Protein × 13 PDB declaration: pentadecameric（15） Consistent with all polymers |
Chain R
1–76（76 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 2.80 Å |
| 9V9R Cryo-EM structure of the ncPRC1.1-UbcH5c E3-E2 complex bound to the H2BK120ub-modified nucleosome Deposited 2025-06-02 | Assembly 1 Protein–DNA Heteromer；Protein × 13 PDB declaration: pentadecameric（15） Consistent with all polymers |
Chain R
1–76（76 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 3.20 Å |