Current Protein Identity：P62987
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 11TA Cryo-EM structure of substrate engaged p97-Ufd1-NPL4-Faf1 complex (motor focused) Deposited 2026-03-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric（12） Consistent with protein count |
Chain I
1–76（76 aa）
Chain K
1–76（76 aa）
|
Not recorded | ZN ZINC ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6；50 mM HEPES, pH 7.6, 150 mM KCl, 5 mM MgCl2, 2 mM ATP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.58 Å |
| 22TU Human 80S ribosome in complex with DHX29 Deposited 2026-01-22 | Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 191 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 28LN XBP1u-stalled RPL4 RNC in complex with NAC Deposited 2026-02-05 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Bm
1–128（128 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 2.70 Å |
| 2LJ5 Description of the Structural fluctuations of proteins from structure-based calculations of Residual dipolar couplings Deposited 2011-09-06 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–76（76 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；300 K；Ionic strength (raw mmCIF value) 200；Pressure ambient
NMR sample composition
200 mM protein, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2MJB Solution nmr structure of ubiquitin refined against dipolar couplings in 4 media Deposited 2014-01-02 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
229–304（76 aa）
Fragment：Ubiquitin-like 3 domain
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6；308 K；Pressure ambient
NMR measurement conditions
pH 6；298 K；Pressure ambient
NMR sample composition
0.5 mM [U-100% 13C; U-100% 15N; U-100% 2H] ubiquitin, 20 mM [U-100% 2H] D4-imidazole, 4.8 mM squalamine, 1.5 mM hexanol, 10 mM sodium phosphate, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
0.5 mM [U-100% 13C; U-100% 15N; U-100% 2H] ubiquitin, 20 mM [U-100% 2H] D4-imidazole, 1.5 mM hexanol, 10 mM sodium phosphate, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
0.6 mM [U-100% 13C; U-100% 15N] ubiquitin, 13 mg/mL Pf1 phage, 140 mM sodium chloride, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
0.8 mM [U-100% 13C; U-100% 15N; U-100% 2H] ubiquitin, 14 mg/mL Pf1 phage, 150 mM sodium chloride, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 2MUR Solution Structure of the Human FAAP20 UBZ-Ubiquitin Complex Deposited 2014-09-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–76（76 aa）
|
Not recorded | ZN ZINC ION × 1 | SOLUTION NMR |
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 100；Pressure ambient
NMR sample composition
1 mM [U-100% 13C; U-100% 15N] Ubiquitin, 25 mM sodium phosphate, 100 mM potassium chloride, 2 mM UBZ, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.8 mM [U-100% 13C; U-100% 15N] UBZ, 0.8 mM [U-100% 13C; U-100% 15N] Ubiquitin, 25 mM sodium phosphate, 100 mM potassium chloride, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
3 mM [U-100% 13C; U-100% 15N] Ubiquitin, 25 mM sodium phosphate, 100 mM potassium chloride, 3 mM [U-100% 13C; U-100% 15N] UBZ, 100% D2O | 100% D2O
NMR sample composition
3 mM Ubiquitin, 25 mM sodium phosphate, 100 mM potassium chloride, 3 mM [U-100% 13C; U-100% 15N] UBZ, 100% D2O | 100% D2O
NMR sample composition
3 mM [U-100% 13C; U-100% 15N] Ubiquitin, 25 mM sodium phosphate, 100 mM potassium chloride, 3 mM UBZ, 100% D2O | 100% D2O
NMR sample composition
1 mM [U-100% 13C; U-100% 15N] Ubiquitin, 25 mM sodium phosphate, 100 mM potassium chloride, 2 mM UBZ, 100% D2O | 100% D2O
NMR sample composition
0.8 mM [U-100% 13C; U-100% 15N] UBZ, 0.8 mM [U-100% 13C; U-100% 15N] Ubiquitin, 25 mM sodium phosphate, 100 mM potassium chloride, 100% D2O | 100% D2O
|
Resolution not provided |
| 2N3U Solution structure of the Rpn1 T1 site engaging two monoubiquitin molecules Deposited 2015-06-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–76（76 aa）
Fragment：UNP residues 1-76
Chain C
1–76（76 aa）
Fragment：UNP residues 1-76
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.7；298 K；Ionic strength (raw mmCIF value) 50；Pressure ambient
NMR sample composition
0.6 mM [U-100% 15N] protein_1, 0.6 mM protein_2, 50 mM HEPES, 50 mM sodium chloride, 2 mM DTT, 1 mM EDTA, 0.1 % sodium azide, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
0.5 mM protein_1, 0.5 mM [U-100% 15N] protein_2, 50 mM HEPES, 50 mM sodium chloride, 2 mM DTT, 1 mM EDTA, 0.1 % sodium azide, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
0.6 mM [U-13C] protein_1, 0.6 mM protein_2, 50 mM HEPES, 50 mM sodium chloride, 2 mM DTT, 1 mM EDTA, 0.1 % sodium azide, 100% D2O | 100% D2O
NMR sample composition
0.6 mM protein_1, 0.6 mM [U-13C] protein_2, 50 mM HEPES, 50 mM sodium chloride, 2 mM DTT, 1 mM EDTA, 0.1 % sodium azide, 100% D2O | 100% D2O
|
Resolution not provided |
| 2N3V Solution structure of the Rpn1 T1 site with K48-linked diubiquitin in the extended binding mode Deposited 2015-06-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–76（76 aa）
Fragment：UNP residues 1-76
Chain C
1–76（76 aa）
Fragment：UNP residues 1-76
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.7；298 K；Ionic strength (raw mmCIF value) 50；Pressure AMBIENT
NMR sample composition
0.3 MM [U-100% 15N] PROTEIN_1, 0.04-1.2 MM PROTEIN_2, 50 MM HEPES, 50 MM SODIUM CHLORIDE, 1 MM EDTA, 2 MM DTT, 0.1 % SODIUM AZIDE, 95% H2O/5% D2O
NMR sample composition
0.08-0.3 MM PROTEIN_1, 0.3 MM [U-100% 15N] PROTEIN_2, 50 MM HEPES, 50 MM SODIUM CHLORIDE, 1 MM EDTA, 2 MM DTT, 0.1 % SODIUM AZIDE, 95% H2O/ 5% D2O
NMR sample composition
0.06-0.5 MM PROTEIN_1, 0.5 MM [U-13C] PROTEIN_2, 50 MM HEPES, 50 MM SODIUM CHLORIDE, 1 MM EDTA, 2 MM DTT, 0.1 % SODIUM AZIDE, 100% D2O
NMR sample composition
0.6 MM [U-13C] PROTEIN_1, 0.6 MM PROTEIN_2, 50 MM HEPES, 50 MM SODIUM CHLORIDE, 1 MM EDTA, 2 MM DTT, 0.1 % SODIUM AZIDE, 100% D2O
NMR sample composition
0.5 MM PROTEIN_ 1, 0.5 MM [U-13C] PROTEIN_2, 50 MM HEPES, 50 MM SODIUM CHLORIDE, 1 MM EDTA, 2 MM DTT, 0.1 % SODIUM AZIDE, 100% D2O
NMR sample composition
0.6 MM [U-13C] PROTEIN_1, 0.12-0.6 MM PROTEIN_2, 50 MM HEPES, 50 MM SODIUM CHLORIDE, 1 MM EDTA, 2 MM DTT, 0.1 % SODIUM AZIDE, 100% D2O
|
Resolution not provided |
| 2N3W Solution structure of the Rpn1 T1 site with K48-linked diubiquitin in the contracted binding mode Deposited 2015-06-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–76（76 aa）
Fragment：UNP residues 1-76
Chain C
1–76（76 aa）
Fragment：UNP residues 1-76
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.7；298 K；Ionic strength (raw mmCIF value) 50；Pressure ambient
NMR sample composition
0.3 mM [U-100% 15N] protein_1, 0.04-1.2 mM protein_2, 50 mM HEPES, 50 mM sodium chloride, 2 mM DTT, 1 mM EDTA, 0.1 % sodium azide, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
0.08-0.3 mM protein_1, 0.3 mM [U-100% 15N] protein_2, 50 mM HEPES, 50 mM sodium chloride, 2 mM DTT, 1 mM EDTA, 0.1 % sodium azide, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
0.06-0.5 mM protein_1, 0.5 mM [U-13C] protein_2, 50 mM HEPES, 50 mM sodium chloride, 2 mM DTT, 1 mM EDTA, 0.1 % sodium azide, 100% D2O | 100% D2O
NMR sample composition
0.6 mM [U-13C] protein_1, 0.6 mM protein_2, 50 mM HEPES, 50 mM sodium chloride, 2 mM DTT, 1 mM EDTA, 0.1 % sodium azide, 100% D2O | 100% D2O
NMR sample composition
0.5 mM protein_1, 0.5 mM [U-13C] protein_2, 50 mM HEPES, 50 mM sodium chloride, 2 mM DTT, 1 mM EDTA, 0.1 % sodium azide, 100% D2O | 100% D2O
NMR sample composition
0.6 mM [U-13C] protein_1, 0.12-0.6 mM protein_2, 50 mM HEPES, 50 mM sodium chloride, 2 mM DTT, 1 mM EDTA, 0.1 % sodium azide, 100% D2O | 100% D2O
|
Resolution not provided |
| 4JIO Bro1 V domain and ubiquitin Deposited 2013-03-06 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain U
1–76（76 aa）
|
Mutation：A28M | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；0.1 M bis-Tris propane pH 7, 0.2-0.4 M sodium malonate, 15-20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 3.60 Å R-free 0.421 |
| 4P4H Caught-in-action signaling complex of RIG-I 2CARD domain and MAVS CARD domain Deposited 2014-03-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 21 PDB declaration: 21-meric（21） Consistent with protein count |
Chain S
1–76（76 aa）
Chain T
1–76（76 aa）
Chain U
1–76（76 aa）
Chain W
1–76（76 aa）
Chain X
1–76（76 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；Tri-Li Citrate, PEG 3350, Cr(III) Cl3
|
Resolution 3.40 Å R-free 0.240 |
| 4PIG Crystal structure of the ubiquitin K11S mutant Deposited 2014-05-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–76（76 aa）
|
Mutation：K11S | CA CALCIUM ION × 1 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；0.2M CaCl2, 0.1 M HEPES, 30% PEG 4000
|
Resolution 1.95 Å R-free 0.217 |
| 4PIG Crystal structure of the ubiquitin K11S mutant Deposited 2014-05-08 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–76（76 aa）
|
Mutation：K11S | CA CALCIUM ION × 1 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；0.2M CaCl2, 0.1 M HEPES, 30% PEG 4000
|
Resolution 1.95 Å R-free 0.217 |
| 4PIG Crystal structure of the ubiquitin K11S mutant Deposited 2014-05-08 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
1–76（76 aa）
|
Mutation：K11S | CL CHLORIDE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；0.2M CaCl2, 0.1 M HEPES, 30% PEG 4000
|
Resolution 1.95 Å R-free 0.217 |
| 4PIG Crystal structure of the ubiquitin K11S mutant Deposited 2014-05-08 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
1–76（76 aa）
|
Mutation：K11S | CA CALCIUM ION × 2 CL CHLORIDE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；0.2M CaCl2, 0.1 M HEPES, 30% PEG 4000
|
Resolution 1.95 Å R-free 0.217 |
| 4PIG Crystal structure of the ubiquitin K11S mutant Deposited 2014-05-08 | Assembly 5 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–76（76 aa）
Chain B
1–76（76 aa）
Chain C
1–76（76 aa）
Chain D
1–76（76 aa）
|
Mutation：K11S Mutation：K11S Mutation：K11S Mutation：K11S | CA CALCIUM ION × 4 CL CHLORIDE ION × 8 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；0.2M CaCl2, 0.1 M HEPES, 30% PEG 4000
|
Resolution 1.95 Å R-free 0.217 |
| 4PIH X-ray crystal structure of the K33S mutant of ubiquitin Deposited 2014-05-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–76（76 aa）
|
Mutation：K33S | CA CALCIUM ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；0.2M CaCl2, 0.1 M TRIS, 25% PEG 4000
|
Resolution 1.50 Å R-free 0.190 |
| 4PIH X-ray crystal structure of the K33S mutant of ubiquitin Deposited 2014-05-08 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–76（76 aa）
|
Mutation：K33S | CA CALCIUM ION × 2 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；0.2M CaCl2, 0.1 M TRIS, 25% PEG 4000
|
Resolution 1.50 Å R-free 0.190 |
| 4PIH X-ray crystal structure of the K33S mutant of ubiquitin Deposited 2014-05-08 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–76（76 aa）
Chain B
1–76（76 aa）
|
Mutation：K33S Mutation：K33S | CA CALCIUM ION × 3 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；0.2M CaCl2, 0.1 M TRIS, 25% PEG 4000
|
Resolution 1.50 Å R-free 0.190 |
| 4PIH X-ray crystal structure of the K33S mutant of ubiquitin Deposited 2014-05-08 | Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–76（76 aa）
Chain B
1–76（76 aa）
|
Mutation：K33S Mutation：K33S | CA CALCIUM ION × 3 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；0.2M CaCl2, 0.1 M TRIS, 25% PEG 4000
|
Resolution 1.50 Å R-free 0.190 |
| 4PIJ X-ray crystal structure of the K11S/K63S double mutant of ubiquitin Deposited 2014-05-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–75（75 aa）
|
Mutation：K11S, K63S | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；291 K；0.2M ammonium sulfate, 25% PEG 4000
|
Resolution 1.50 Å R-free 0.198 |
| 4PIJ X-ray crystal structure of the K11S/K63S double mutant of ubiquitin Deposited 2014-05-08 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–75（75 aa）
|
Mutation：K11S, K63S | SO4 SULFATE ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；291 K；0.2M ammonium sulfate, 25% PEG 4000
|
Resolution 1.50 Å R-free 0.198 |
| 4PIJ X-ray crystal structure of the K11S/K63S double mutant of ubiquitin Deposited 2014-05-08 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–75（75 aa）
Chain B
1–75（75 aa）
|
Mutation：K11S, K63S Mutation：K11S, K63S | GOL GLYCEROL × 1 SO4 SULFATE ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；291 K；0.2M ammonium sulfate, 25% PEG 4000
|
Resolution 1.50 Å R-free 0.198 |
| 4PIJ X-ray crystal structure of the K11S/K63S double mutant of ubiquitin Deposited 2014-05-08 | Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–75（75 aa）
Chain B
1–75（75 aa）
|
Mutation：K11S, K63S Mutation：K11S, K63S | GOL GLYCEROL × 1 SO4 SULFATE ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；291 K；0.2M ammonium sulfate, 25% PEG 4000
|
Resolution 1.50 Å R-free 0.198 |
| 4PIJ X-ray crystal structure of the K11S/K63S double mutant of ubiquitin Deposited 2014-05-08 | Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–75（75 aa）
Chain B
1–75（75 aa）
|
Mutation：K11S, K63S Mutation：K11S, K63S | GOL GLYCEROL × 1 SO4 SULFATE ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.5；291 K；0.2M ammonium sulfate, 25% PEG 4000
|
Resolution 1.50 Å R-free 0.198 |
| 4RF0 Crystal structure of the Middle-East respiratory syndrome coronavirus papain-like protease in complex with ubiquitin (space group P6522) Deposited 2014-09-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–75（75 aa）
Fragment：UNP residues 1-75
|
Not recorded | ZN ZINC ION × 1 SO4 SULFATE ION × 9 3CN 3-AMINOPROPANE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；1.80 M ammonium sulphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.281 |
| 4RF1 Crystal structure of the Middle-East respiratory syndrome coronavirus papain-like protease in complex with ubiquitin (space group P63) Deposited 2014-09-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–75（75 aa）
Fragment：UNP residues 1-75
|
Not recorded | ZN ZINC ION × 1 PGO S-1,2-PROPANEDIOL × 5 3CN 3-AMINOPROPANE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；293 K；20% PEG 4000, 0.1 M trisodium citrate, 20% isopropanol, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.15 Å R-free 0.231 |
| 4S1Z Crystal structure of TRABID NZF1 in complex with K29 linked di-Ubiquitin Deposited 2015-01-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–76（76 aa）
Fragment：residues 1-76
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；285 K；100mM MES, 200mM potassium iodide and 25% PEG4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K
|
Resolution 3.03 Å R-free 0.270 |
| 4S1Z Crystal structure of TRABID NZF1 in complex with K29 linked di-Ubiquitin Deposited 2015-01-16 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–76（76 aa）
Fragment：residues 1-76
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；285 K；100mM MES, 200mM potassium iodide and 25% PEG4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K
|
Resolution 3.03 Å R-free 0.270 |
| 4S1Z Crystal structure of TRABID NZF1 in complex with K29 linked di-Ubiquitin Deposited 2015-01-16 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1–76（76 aa）
Fragment：residues 1-76
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；285 K；100mM MES, 200mM potassium iodide and 25% PEG4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K
|
Resolution 3.03 Å R-free 0.270 |
| 4S1Z Crystal structure of TRABID NZF1 in complex with K29 linked di-Ubiquitin Deposited 2015-01-16 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–76（76 aa）
Fragment：residues 1-76
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；285 K；100mM MES, 200mM potassium iodide and 25% PEG4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K
|
Resolution 3.03 Å R-free 0.270 |
| 4S1Z Crystal structure of TRABID NZF1 in complex with K29 linked di-Ubiquitin Deposited 2015-01-16 | Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
1–76（76 aa）
Fragment：residues 1-76
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；285 K；100mM MES, 200mM potassium iodide and 25% PEG4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K
|
Resolution 3.03 Å R-free 0.270 |
| 4UG0 STRUCTURE OF THE HUMAN 80S RIBOSOME Deposited 2015-03-20 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 89-meric（89） Review required |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 239 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 4V6X Structure of the human 80S ribosome Deposited 2013-02-27 | Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric（89） Consistent with all polymers |
Chain Cm
77–128（52 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 3 seconds using two pieces of filter paper, then plunge into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 5.00 Å |
| 4XKL Crystal structure of NDP52 ZF2 in complex with mono-ubiquitin Deposited 2015-01-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–76（76 aa）
|
Not recorded | GOL GLYCEROL × 1 ACT ACETATE ION × 2 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；289 K；0.2 M magnesium acetate tetrahydrate, 20% w/v Polyethylene glycol 3350
|
Resolution 2.10 Å R-free 0.243 |
| 4XKL Crystal structure of NDP52 ZF2 in complex with mono-ubiquitin Deposited 2015-01-12 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1–76（76 aa）
|
Not recorded | GOL GLYCEROL × 1 ACT ACETATE ION × 2 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；289 K；0.2 M magnesium acetate tetrahydrate, 20% w/v Polyethylene glycol 3350
|
Resolution 2.10 Å R-free 0.243 |
| 5AJ0 Cryo electron microscopy of actively translating human polysomes (POST state). Deposited 2015-02-19 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Am
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 326 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
20 MM HEPES-KOH, PH 7.5, 100 MM KCL, 1.5 MM MGCL2, 0.5 MM SPERMIDINE, 0.04 MM SPERMINE, 1 MM DTT；pH 7.5；20 MM HEPES-KOH, PH 7.5, 100 MM KCL, 1.5 MM MGCL2, 0.5 MM SPERMIDINE, 0.04 MM SPERMINE, 1 MM DTT
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 93, INSTRUMENT- FEI VITROBOT MARK II, METHOD- BLOT FOR 2-4 SECONDS BEFORE PLUNGING,
|
Resolution 3.50 Å |
| 5LKS Structure-function insights reveal the human ribosome as a cancer target for antibiotics Deposited 2016-07-23 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 80-meric（80） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 MG MAGNESIUM ION × 224 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 5T2C CryoEM structure of the human ribosome at 3.6 Angstrom resolution Deposited 2016-08-23 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric（81） Consistent with all polymers |
Chain g
1–128（128 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6IP5 Cryo-EM structure of the CMV-stalled human 80S ribosome (Structure ii) Deposited 2018-11-02 | Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain 2g
1–128（128 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6IP6 Cryo-EM structure of the CMV-stalled human 80S ribosome with HCV IRES (Structure iii) Deposited 2018-11-02 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain 2g
1–128（128 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 6IP8 Cryo-EM structure of the HCV IRES dependently initiated CMV-stalled 80S ribosome (Structure iv) Deposited 2018-11-02 | Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain 2g
1–128（128 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6LQM Cryo-EM structure of a pre-60S ribosomal subunit - state C Deposited 2020-01-14 | Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 48-meric（48） Consistent with all polymers |
Chain R
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 251 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.09 Å |
| 6OLE Human ribosome nascent chain complex (CDH1-RNC) stalled by a drug-like molecule with AP and PE tRNAs Deposited 2019-04-16 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain n
78–127（50 aa）
|
Not recorded | MG MAGNESIUM ION × 31 ZN ZINC ION × 6 MVM N-(3-chloropyridin-2-yl)-N-[(3R)-piperidin-3-yl]-4-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)benzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6OLF Human ribosome nascent chain complex (CDH1-RNC) stalled by a drug-like molecule with AA and PE tRNAs Deposited 2019-04-16 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain n
78–127（50 aa）
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 31 MVM N-(3-chloropyridin-2-yl)-N-[(3R)-piperidin-3-yl]-4-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)benzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6OLG Human ribosome nascent chain complex stalled by a drug-like small molecule (CDH1_RNC with PP tRNA) Deposited 2019-04-16 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Am
78–127（50 aa）
|
Not recorded | MG MAGNESIUM ION × 326 ZN ZINC ION × 5 MVM N-(3-chloropyridin-2-yl)-N-[(3R)-piperidin-3-yl]-4-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)benzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 6OLI Structure of human ribosome nascent chain complex selectively stalled by a drug-like small molecule (USO1-RNC) Deposited 2019-04-16 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain n
78–127（50 aa）
|
Not recorded | MG MAGNESIUM ION × 31 ZN ZINC ION × 6 MVM N-(3-chloropyridin-2-yl)-N-[(3R)-piperidin-3-yl]-4-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)benzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6OLZ Human ribosome nascent chain complex (PCSK9-RNC) stalled by a drug-like molecule with PP tRNA Deposited 2019-04-17 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain Am
78–127（50 aa）
|
Not recorded | MG MAGNESIUM ION × 326 ZN ZINC ION × 5 MVM N-(3-chloropyridin-2-yl)-N-[(3R)-piperidin-3-yl]-4-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)benzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6OM0 Human ribosome nascent chain complex (PCSK9-RNC) stalled by a drug-like molecule with AP and PE tRNAs Deposited 2019-04-17 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain n
78–127（50 aa）
|
Not recorded | MG MAGNESIUM ION × 31 ZN ZINC ION × 6 MVM N-(3-chloropyridin-2-yl)-N-[(3R)-piperidin-3-yl]-4-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)benzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6OM7 Human ribosome nascent chain complex (PCSK9-RNC) stalled by a drug-like small molecule with AA and PE tRNAs Deposited 2019-04-18 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain n
78–127（50 aa）
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 31 MVM N-(3-chloropyridin-2-yl)-N-[(3R)-piperidin-3-yl]-4-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)benzamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6QZP High-resolution cryo-EM structure of the human 80S ribosome Deposited 2019-03-12 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric（81） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 438 HMT (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine × 1 ZN ZINC ION × 8 HYG HYGROMYCIN B × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 6XA1 Structure of a drug-like compound stalled human translation termination complex Deposited 2020-06-03 | Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 190 MVM N-(3-chloropyridin-2-yl)-N-[(3R)-piperidin-3-yl]-4-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)benzamide × 1 ZN ZINC ION × 8 K POTASSIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 6Y0G Structure of human ribosome in classical-PRE state Deposited 2020-02-07 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 72 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6Y2L Structure of human ribosome in POST state Deposited 2020-02-16 | Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 80-meric（80） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 193 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 6Y57 Structure of human ribosome in hybrid-PRE state Deposited 2020-02-25 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 160 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6Y6X Tetracenomycin X bound to the human ribosome Deposited 2020-02-27 | Assembly 1 Protein–RNA Heteromer；Protein × 43 PDB declaration: 46-meric（46） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 229 OCW Tetracenomycin X × 1 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 6Z6L Cryo-EM structure of human CCDC124 bound to 80S ribosomes Deposited 2020-05-28 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 256 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 6Z6M Cryo-EM structure of human 80S ribosomes bound to EBP1, eEF2 and SERBP1 Deposited 2020-05-28 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 256 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6Z6N Cryo-EM structure of human EBP1-80S ribosomes (focus on EBP1) Deposited 2020-05-28 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 256 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 6ZM7 SARS-CoV-2 Nsp1 bound to the human CCDC124-80S-EBP1 ribosome complex Deposited 2020-07-01 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 256 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 6ZME SARS-CoV-2 Nsp1 bound to the human CCDC124-80S-eERF1 ribosome complex Deposited 2020-07-02 | Assembly 1 Protein–RNA Heteromer；Protein × 83 PDB declaration: 88-meric（88） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 256 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 6ZMI SARS-CoV-2 Nsp1 bound to the human LYAR-80S ribosome complex Deposited 2020-07-02 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 256 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 6ZMO SARS-CoV-2 Nsp1 bound to the human LYAR-80S-eEF1a ribosome complex Deposited 2020-07-03 | Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric（88） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 256 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 7AY1 Cryo-EM structure of USP1-UAF1 bound to mono-ubiquitinated FANCD2, and FANCI Deposited 2020-11-10 | Assembly 1 Protein–DNA Heteromer；Protein × 5 PDB declaration: heptameric（7） Consistent with all polymers |
Chain C
1–76（76 aa）
|
Mutation：GPGS expression tag | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 7BHP Cryo-EM structure of the human Ebp1 - 80S ribosome Deposited 2021-01-11 | Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 45-meric（45） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 41 ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 7F5S human delta-METTL18 60S ribosome Deposited 2021-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric（45） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 279 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.72 Å |
| 7M3Q Structure of the Smurf2 HECT Domain with a High Affinity Ubiquitin Variant (UbV) Deposited 2021-03-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
2–74（73 aa）
|
Not recorded | SO4 SULFATE ION × 2 NA SODIUM ION × 2 CL CHLORIDE ION × 6 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 EDO 1,2-ETHANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；1.5 M Ammonium Sulphate and 100 mM MES pH 6.0. Trypsin was added to the protein complex at a final concentration of 10 microgram per mL
|
Resolution 2.50 Å R-free 0.235 |
| 7OWC Structure of CYLD CAP-Gly3 (467-565) bound to Ub; orthorhobic space group Deposited 2021-06-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–76（76 aa）
Chain C
1–76（76 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.3；291 K；35 % (w/v) PEG 1,000, 50 mM HEPES pH 7.3
|
Resolution 1.85 Å R-free 0.239 |
| 7UN3 Complex of UBE2O with NAP1L1 and ubiquitylated uL2 Deposited 2022-04-08 | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–76（76 aa）
Chain D
1–76（76 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 7XNX High resolution cry-EM structure of the human 80S ribosome from SNORD127+/+ Kasumi-1 cells Deposited 2022-04-30 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric（81） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 421 K POTASSIUM ION × 12 ZN ZINC ION × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 7XNY High resolution cry-EM structure of the human 80S ribosome from SNORD127+/- Kasumi-1 cells Deposited 2022-04-30 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 79-meric（79） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 421 K POTASSIUM ION × 12 ZN ZINC ION × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 7ZF1 Structure of ubiquitinated FANCI in complex with FANCD2 and double-stranded DNA Deposited 2022-03-31 | Assembly 1 Protein–DNA Heteromer；Protein × 3 PDB declaration: pentameric（5） Consistent with all polymers |
Chain C
1–76（76 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.14 Å |
| 7ZH3 USP1 bound to ubiquitin conjugated to FANCD2 (focused refinement) Deposited 2022-04-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1–76（76 aa）
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；blotted for 3.0 secs before plunging
|
Resolution 2.50 Å |
| 7ZH4 USP1 bound to ML323 and ubiquitin conjugated to FANCD2 (focused refinement) Deposited 2022-04-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1–76（76 aa）
|
Not recorded | ZN ZINC ION × 1 JDA 5-methyl-2-(2-propan-2-ylphenyl)-~{N}-[[4-(1,2,3-triazol-1-yl)phenyl]methyl]pyrimidin-4-amine × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；blotting for 3.0 seconds
|
Resolution 2.49 Å |
| 8ADB Viral tegument-like DUBs Deposited 2022-07-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–75（75 aa）
|
Not recorded | CIT CITRIC ACID × 1 AYE prop-2-en-1-amine × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；0.1 M BisTris propane pH 7.0, 2.0 M Ammonium citrate tribasic
|
Resolution 1.73 Å R-free 0.193 |
| 8CX9 Structure of the SARS-COV2 PLpro (C111S) in complex with a dimeric Ubv that inhibits activity by an unusual allosteric mechanism Deposited 2022-05-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain F
1–77（77 aa）
Chain G
1–77（77 aa）
|
Not recorded | ZN ZINC ION × 1 BR BROMIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.3；281 K；14.86 mg/ml SARS-CoV2C111S/UbV.CV2.1,
20% PEG 3350, 0.2 M sodium bromide, 0.1 M BIS-TRIS propane (pH 6.3), 50 mM lithium chloride. Cryoprotected in the same buffer plus 30% glycerol
|
Resolution 3.50 Å R-free 0.270 |
| 8CX9 Structure of the SARS-COV2 PLpro (C111S) in complex with a dimeric Ubv that inhibits activity by an unusual allosteric mechanism Deposited 2022-05-20 | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain E
1–77（77 aa）
Chain I
1–77（77 aa）
|
Not recorded | ZN ZINC ION × 1 BR BROMIDE ION × 3 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.3；281 K；14.86 mg/ml SARS-CoV2C111S/UbV.CV2.1,
20% PEG 3350, 0.2 M sodium bromide, 0.1 M BIS-TRIS propane (pH 6.3), 50 mM lithium chloride. Cryoprotected in the same buffer plus 30% glycerol
|
Resolution 3.50 Å R-free 0.270 |
| 8CX9 Structure of the SARS-COV2 PLpro (C111S) in complex with a dimeric Ubv that inhibits activity by an unusual allosteric mechanism Deposited 2022-05-20 | Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain K
1–77（77 aa）
Chain L
1–77（77 aa）
|
Not recorded | ZN ZINC ION × 1 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.3；281 K；14.86 mg/ml SARS-CoV2C111S/UbV.CV2.1,
20% PEG 3350, 0.2 M sodium bromide, 0.1 M BIS-TRIS propane (pH 6.3), 50 mM lithium chloride. Cryoprotected in the same buffer plus 30% glycerol
|
Resolution 3.50 Å R-free 0.270 |
| 8CX9 Structure of the SARS-COV2 PLpro (C111S) in complex with a dimeric Ubv that inhibits activity by an unusual allosteric mechanism Deposited 2022-05-20 | Assembly 4 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain H
1–77（77 aa）
Chain J
1–77（77 aa）
|
Not recorded | ZN ZINC ION × 1 BR BROMIDE ION × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.3；281 K；14.86 mg/ml SARS-CoV2C111S/UbV.CV2.1,
20% PEG 3350, 0.2 M sodium bromide, 0.1 M BIS-TRIS propane (pH 6.3), 50 mM lithium chloride. Cryoprotected in the same buffer plus 30% glycerol
|
Resolution 3.50 Å R-free 0.270 |
| 8IFD Dibekacin-added human 80S ribosome Deposited 2023-02-17 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 80-meric（80） Consistent with all polymers |
Chain 2g
1–128（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 84D Dibekacin × 24 MG MAGNESIUM ION × 397 ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.59 Å |
| 8IFE Arbekacin-added human 80S ribosome Deposited 2023-02-17 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 80-meric（80） Consistent with all polymers |
Chain 2g
1–128（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | 84G Arbekacin × 23 MG MAGNESIUM ION × 410 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.57 Å |
| 8IK6 pUbl depleted Parkin complex with pUbiquitin Deposited 2023-02-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–75（75 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 8 SO4 SULFATE ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；1.6 M Ammonium sulfate, 0.1 M MES monohydrate pH 6.5, 10% v/v 1,4-Dioxane
|
Resolution 3.30 Å R-free 0.270 |
| 8IK6 pUbl depleted Parkin complex with pUbiquitin Deposited 2023-02-28 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–75（75 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 6 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；1.6 M Ammonium sulfate, 0.1 M MES monohydrate pH 6.5, 10% v/v 1,4-Dioxane
|
Resolution 3.30 Å R-free 0.270 |
| 8IKM Trans complex of phospho parkin Deposited 2023-02-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–75（75 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 6 GOL GLYCEROL × 1 PEG DI(HYDROXYETHYL)ETHER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；0.3 M Sodium nitrate, 0.3 Sodium phosphate dibasic, 0.3 M Ammonium sulfate,1.0 M 8.5 Tris (base), BICINE, 25% v/v MPD; 25% PEG 1000, 25% w/v PEG 3350
|
Resolution 1.92 Å R-free 0.233 |
| 8IKT Ternary trans-complex of phospho-parkin with cis ACT and pUb Deposited 2023-03-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–75（75 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 6 GOL GLYCEROL × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 3CN 3-AMINOPROPANE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；0.3 M Sodium nitrate, 0.3 Sodium phosphate dibasic, 0.3 M Ammonium sulfate, Imidazole, MES monohydrate (acid), 25% v/v MPD, 25% PEG 1000, 25% w/v PEG 3350
|
Resolution 2.60 Å R-free 0.236 |
| 8IKV pUbl depleted phospho-Parkin(K211N,R163D) in complex with pUb Deposited 2023-03-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–75（75 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 8 PEG DI(HYDROXYETHYL)ETHER × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；0.15 M Potassium bromide, 30% w/v Polyethylene glycol monomethyl ether 2,000
|
Resolution 2.35 Å R-free 0.217 |
| 8IKV pUbl depleted phospho-Parkin(K211N,R163D) in complex with pUb Deposited 2023-03-01 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–75（75 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 8 PEG DI(HYDROXYETHYL)ETHER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；0.15 M Potassium bromide, 30% w/v Polyethylene glycol monomethyl ether 2,000
|
Resolution 2.35 Å R-free 0.217 |
| 8IPJ Crystal structure of the Legionella effector protein MavL with ADPR-Ub Deposited 2023-03-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–72（72 aa）
|
Not recorded | AR6 [(2R,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-DIHYDROXY-OXOLAN-2-YL]METHYL[HYDROXY-[[(2R,3S,4R,5S)-3,4,5-TRIHYDROXYOXOLAN-2-YL]METHOXY]PHOSPHORYL] HYDROGEN PHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
COUNTER-DIFFUSION；289.15 K；0.1 M HEPES/sodium hydroxide pH7.5, 20% polyethylene glycol 10000
|
Resolution 2.00 Å R-free 0.222 |
| 8JDJ Structure of the Human cytoplasmic Ribosome with human tRNA Asp(Q34) and mRNA(GAU) Deposited 2023-05-14 | Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 80-meric（80） Consistent with all polymers |
Chain r
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 539 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；The Buffer pH was adjusted to 7.4 using KOH.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 8JDK Structure of the Human cytoplasmic Ribosome with human tRNA Asp(ManQ34) and mRNA(GAU) Deposited 2023-05-14 | Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 80-meric（80） Consistent with all polymers |
Chain r
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 539 MAN alpha-D-mannopyranose × 2 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；The Buffer pH was adjusted to 7.4 using KOH.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.26 Å |
| 8JDL Structure of the Human cytoplasmic Ribosome with human tRNA Tyr(GalQ34) and mRNA(UAU) (non-rotated state) Deposited 2023-05-14 | Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric（79） Consistent with all polymers |
Chain r
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 539 GAL beta-D-galactopyranose × 1 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；The Buffer pH was adjusted to 7.4 using KOH.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.42 Å |
| 8JDM Structure of the Human cytoplasmic Ribosome with human tRNA Tyr(GalQ34) and mRNA(UAU) (rotated state) Deposited 2023-05-14 | Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 80-meric（80） Consistent with all polymers |
Chain r
1–128（128 aa）
|
Not recorded | GAL beta-D-galactopyranose × 2 MG MAGNESIUM ION × 520 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；The Buffer pH was adjusted to 7.4 using KOH.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.67 Å |
| 8K2C Cryo-EM structure of the human 80S ribosome with Tigecycline Deposited 2023-07-12 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 259 T1C TIGECYCLINE × 9 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 8OHD 60S ribosomal subunit bound to the E3-UFM1 complex - state 3 (native) Deposited 2023-03-21 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric（50） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 220 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8OJ0 60S ribosomal subunit bound to the E3-UFM1 complex - state 2 (native) Deposited 2023-03-23 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 220 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8OJ5 60S ribosomal subunit bound to the E3-UFM1 complex - state 3 (in-vitro reconstitution) Deposited 2023-03-23 | Assembly 1 Protein–RNA Heteromer；Protein × 46 PDB declaration: 49-meric（49） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 220 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8OJ8 60S ribosomal subunit bound to the E3-UFM1 complex - state 1 (native) Deposited 2023-03-24 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric（50） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 220 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8QFD UFL1 E3 ligase bound 60S ribosome Deposited 2023-09-04 | Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric（45） Consistent with all polymers |
Chain m
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 161 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；25 mM HEPES pH 7.5, 50 mM KCl, 5 mM MgCl2, 2 mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å |
| 8QOI Structure of the human 80S ribosome at 1.9 A resolution - the molecular role of chemical modifications and ions in RNA Deposited 2023-09-29 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 79-meric（79） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 292 K POTASSIUM ION × 108 SPD SPERMIDINE × 2 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6；Titan Krios sample is prepared with Sodium cacodylate buffer.
CryoARM 300 sample is prepared with Hepes buffer.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.90 Å |
| 8QYX Human 60S ribosomal subunit Deposited 2023-10-26 | Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 44-meric（44） Consistent with all polymers |
Chain g1
1–128（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 186 PUT 1,4-DIAMINOBUTANE × 7 K POTASSIUM ION × 46 ZN ZINC ION × 5 SPD SPERMIDINE × 7 SPM SPERMINE × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.78 Å |
| 8UKB In situ HHT and CHX treated human hibernating state without E-tRNA 80S ribosome Deposited 2023-10-12 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 255 HMT (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine × 1 WV3 4-{(2R)-2-[(1R,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.05 Å |
| 8XSX Cryo-EM structure of the human 80S ribosome with Tigecycline, E-tRNA, SERBP1 and eEF2 Deposited 2024-01-10 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 259 T1C TIGECYCLINE × 7 ZN ZINC ION × 8 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 8XSY Cryo-EM structure of the human 80S ribosome with Tigecycline, e-tRNA and CCDC124 (40S head Swivelled) Deposited 2024-01-10 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 255 T1C TIGECYCLINE × 8 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8XSZ Cryo-EM structure of the human 80S ribosome with Tigecycline, E-tRNA and P-tRNA Deposited 2024-01-10 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 248 T1C TIGECYCLINE × 5 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8Y0W dormant ribosome with eIF5A, eEF2 and SERBP1 Deposited 2024-01-23 | Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 81-meric（81） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 226 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；50mM HEPES, pH7.5, 100mM KOAc, 5mM Mg(OAc)2, 1mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8Y0X Dormant ribosome with SERBP1 Deposited 2024-01-23 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 80-meric（80） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 255 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；50mM HEPES, pH7.4, 100mM KoAc, 5mM Mg(OAc)2, 1mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8YOO Cryo-EM structure of the human 80S ribosome with 100 um Tigecycline Deposited 2024-03-13 | Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 81-meric（81） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 257 T1C TIGECYCLINE × 6 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.00 Å |
| 8YOP Cryo-EM structure of the human 80S ribosome with 4 um Tigecycline Deposited 2024-03-13 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 255 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å |
| 9B0P In situ human Hibernating class1 80S ribosome Deposited 2024-03-12 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 255 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.82 Å |
| 9B0Q In situ human top-back di-ribosome structure (Composite map) Deposited 2024-03-12 | Assembly 1 Protein–RNA Heteromer；Protein × 160 PDB declaration: 172-meric（172） Consistent with all polymers |
Chain Lm
77–128（52 aa）
Chain lm
77–128（52 aa）
|
Not recorded | ZN ZINC ION × 12 MG MAGNESIUM ION × 510 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9B0S In situ human top-top di-ribosome structure (Composite map) Deposited 2024-03-12 | Assembly 1 Protein–RNA Heteromer；Protein × 158 PDB declaration: 171-meric（171） Consistent with all polymers |
Chain Lm
77–128（52 aa）
Chain lm
77–128（52 aa）
|
Not recorded | ZN ZINC ION × 12 MG MAGNESIUM ION × 510 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 9C3H Structure of the CNOT3-bound human 80S ribosome with tRNA-ARG in the P-site. Deposited 2024-06-01 | Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Lo
1–128（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 326 K POTASSIUM ION × 94 B3P 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.00 Å |
| 9FQZ CRYO-EM STRUCTURE OF HCT15 POLYSOMES BOUND TO EEF2, EBP1, AND SERBP1 Deposited 2024-06-17 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 271 K POTASSIUM ION × 104 ZN ZINC ION × 8 PUT 1,4-DIAMINOBUTANE × 7 HYG HYGROMYCIN B × 1 SPD SPERMIDINE × 8 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ANM ANISOMYCIN × 1 NA SODIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.85 Å |
| 9G8M human 80S ribosome bound by a SKI2-exosome complex Deposited 2024-07-23 | Assembly 1 Insufficient information Heteromer；Protein × 87 PDB declaration: 92-meric（92） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 232 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.30 Å |
| 9GMO eIF6-bound pre-60S large ribosomal subunit incorporating mutant uL16 Deposited 2024-08-29 | Assembly 1 Protein–RNA Heteromer；Protein × 43 PDB declaration: 46-meric（46） Consistent with all polymers |
Chain g
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 227 K POTASSIUM ION × 155 NA SODIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.59 Å |
| 9GUL Structure of FLuc-XBP1u+ stalled human 60S ribosome nascent chain complex Deposited 2024-09-19 | Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 46-meric（46） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 284 ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å |
| 9GY4 60S ribosomal subunit in complex with E3-UFM1 ligase and RQC machinery components NEMF and LTN1 (Composite map) Deposited 2024-10-01 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 219 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9I14 CRYO-EM STRUCTURE OF HCT15 POLYSOMES IN HYBRID-PRE STATE Deposited 2025-01-15 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | K POTASSIUM ION × 40 MG MAGNESIUM ION × 267 NA SODIUM ION × 23 SPD SPERMIDINE × 1 PUT 1,4-DIAMINOBUTANE × 1 ZN ZINC ION × 7 HYG HYGROMYCIN B × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.34 Å |
| 9I2D NMT1-NAC bound human RNC with 10 amino acid ARF1-linker Deposited 2025-01-20 | Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric（88） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 191 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.19 Å |
| 9I2E NMT1-NAC bound human ribosome (combined translational states) Deposited 2025-01-20 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 256 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.95 Å |
| 9KZU Cryo-EM structure of the HCV IRES-dependently initiated CMV-stalled 80S ribosome (non-rotated state) in complexed with eIF3 Deposited 2024-12-11 | Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 92-meric（92） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 81 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9KZX Cryo-EM structure of the HCV IRES-dependently initiated CMV-stalled 80S ribosome (rotated state) in complexed with eIF3 Deposited 2024-12-11 | Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 93-meric（93） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 66 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9M0P Cryo-EM structure of human 80S ribosome in complex with montanine Deposited 2025-02-25 | Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 78-meric（78） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 291 ZN ZINC ION × 6 A1L8F (1S,13S,15S,16S)-16-methoxy-5,7-dioxa-12-azapentacyclo[10.6.1.02,10.04,8.013,18]nonadeca-2,4(8),9,17-tetraen-15-ol × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.78 Å |
| 9N9J Structure of a GRP94 folding intermediate engaged with a CCDC134- and FKBP11-bound secretory translocon Deposited 2025-02-10 | Assembly 1 Protein–RNA Heteromer；Protein × 65 PDB declaration: 70-meric（70） Review required |
Chain Lm
1–128（128 aa）
|
Not recorded | ELU phosphono [(3~{R},6~{E},10~{E})-3,7,11,15-tetramethylhexadeca-6,10,14-trienyl] hydrogen phosphate × 1 MG MAGNESIUM ION × 226 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；HEPES buffer was adjusted to pH 7.4 with potassium hydroxide.
cryo-EM vitrification conditions
Cryogen ETHANE；Three microliters of affinity-purified RTCs were applied to the grid, incubated for 60 seconds, blotted for 7 seconds, drained for 0.5 seconds, and frozen in liquid nitrogen-cooled ethane.
|
Resolution 3.20 Å |
| 9O3V Human 80S ribosome stalled on MYC nascent chain Deposited 2025-04-07 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 352 K POTASSIUM ION × 165 SPD SPERMIDINE × 14 PUT 1,4-DIAMINOBUTANE × 7 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；20mM Tris, 100mM KOAc, 7.5mM MgOAc, 0.25mM Spermidine, 2mM DTT, 0.05% w/v b-OG
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å |
| 9O3W Human 80S ribosome bound to IDB-001 stalled on MYC nascent chain Deposited 2025-04-07 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | K POTASSIUM ION × 170 PUT 1,4-DIAMINOBUTANE × 7 SPD SPERMIDINE × 14 A1B75 (2R,3S,4S)-4-hydroxy-2-[(4-methoxyphenyl)methyl]pyrrolidin-3-yl {2-[(2S)-pyrrolidin-2-yl]ethyl}carbamate × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 MG MAGNESIUM ION × 371 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；20mM Tris, 100mM KOAc, 7.5mM MgOAc, 0.25mM Spermidine, 2mM DTT, 0.05% w/v b-OG, 50uM IDB-001
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.90 Å |
| 9O3Y Human 80S ribosome bound to IDB-002 stalled on FPAK-containing nascent chain Deposited 2025-04-07 | Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | K POTASSIUM ION × 168 SPD SPERMIDINE × 14 PUT 1,4-DIAMINOBUTANE × 7 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 MG MAGNESIUM ION × 332 A1B76 (2R,3S,4S)-4-hydroxy-2-[(4-methoxyphenyl)methyl]pyrrolidin-3-yl [(3-fluorophenyl)methyl]carbamate × 1 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；20mM Tris, 100mM KOAc, 7.5mM MgOAc, 0.25mM Spermidine, 2mM DTT, 0.05% w/v b-OG, 50uM IDB-001
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å |
| 9P6Z In situ human P-Z state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.97 Å |
| 9P72 In situ human P-E state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9P73 In situ human eEF2-A/P-P/E state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 218 ZN ZINC ION × 6 SPM SPERMINE × 7 SPD SPERMIDINE × 10 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.66 Å |
| 9P76 In situ human eEF1A-A/T-P state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.83 Å |
| 9P78 In situ human Hibernating rotate 3 with E-site tRNA state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 218 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9P79 In situ human Hibernating rotate3 with Z site tRNA state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 218 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9P7A In situ human hibernating class2 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 218 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.81 Å |
| 9P7C In situ human Hibernating class3 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 218 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.78 Å |
| 9P7D In situ human Hibernating class4 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 218 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.57 Å |
| 9P7E In situ human Hibernating class5 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 218 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.59 Å |
| 9P7F In situ HHT and CHX treated A-P state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 HMT (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.56 Å |
| 9P7G In situ HHT and CHX treated A-P-Z state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 219 ZN ZINC ION × 6 SPM SPERMINE × 7 SPD SPERMIDINE × 10 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 HMT (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.54 Å |
| 9P7H In situ HHT and CHX treated human eEF1A-A/T-P state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 HMT (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine × 1 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.68 Å |
| 9P7I In situ HHT and CHX treated human eEF1A-A/T-P-Z state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 HMT (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine × 1 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.69 Å |
| 9P7J In situ HHT and CHX treated human P state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 HMT (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine × 1 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.91 Å |
| 9P7K In situ human post eEF1A-A/T-P-E state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9P7L In situ human Post-eEF1A-AT-P state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.92 Å |
| 9P7N In situ human Post-eEF1A-A/T-P-Z state 80S ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.83 Å |
| 9P7O In situ human AT-P-Z state ribosome Deposited 2025-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.65 Å |
| 9P7W In situ human A-P state 80S ribosome Deposited 2025-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 219 ZN ZINC ION × 6 SPM SPERMINE × 7 SPD SPERMIDINE × 10 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.93 Å |
| 9P7X In situ human A-P-Z state 80S ribosome Deposited 2025-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.83 Å |
| 9P7Y In situ human A-P-E state 80S ribosome Deposited 2025-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 219 ZN ZINC ION × 6 SPM SPERMINE × 7 SPD SPERMIDINE × 10 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.75 Å |
| 9P8B In situ human eEF1A-A/T-P-E state 80S ribosome Deposited 2025-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric（86） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.48 Å |
| 9P8C In situ human Hibernating class1 (rotate3) without E tRNA state 80S ribosome Deposited 2025-06-22 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 218 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.11 Å |
| 9P8H In situ human A/P-P/E state 80S ribosome Deposited 2025-06-23 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 218 ZN ZINC ION × 6 SPM SPERMINE × 7 SPD SPERMIDINE × 10 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.98 Å |
| 9P8I In situ HHT and CHX treated human P-Z state 80S ribosome Deposited 2025-06-23 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 3HE 4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione × 1 HMT (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine × 1 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.89 Å |
| 9P9H In situ human unrotated hibernating with CCDC124 state 80S ribosome Deposited 2025-06-24 | Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric（85） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.84 Å |
| 9P9I In situ human unrotated hibernating without CCDC124 state 80S ribosome Deposited 2025-06-24 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.77 Å |
| 9P9J In situ human 60S ribosome Deposited 2025-06-24 | Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 44-meric（44） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 191 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.87 Å |
| 9P9K In situ human 60S ribosome with EIF6 Deposited 2025-06-24 | Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric（45） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 191 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.96 Å |
| 9PA7 In situ human 80S consensus ribosome with EBP1 Deposited 2025-06-25 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric（87） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 225 SPM SPERMINE × 8 SPD SPERMIDINE × 12 K POTASSIUM ION × 75 ZN ZINC ION × 7 PUT 1,4-DIAMINOBUTANE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.67 Å |
| 9PBE In situ human 80S ribosome (composite map) Deposited 2025-06-26 | Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric（84） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 225 SPM SPERMINE × 8 SPD SPERMIDINE × 12 K POTASSIUM ION × 75 ZN ZINC ION × 7 PUT 1,4-DIAMINOBUTANE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.19 Å |
| 9PKG In situ human P state 80S ribosome Deposited 2025-07-14 | Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Lm
77–128（52 aa）
|
Not recorded | MG MAGNESIUM ION × 219 SPM SPERMINE × 7 SPD SPERMIDINE × 10 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.26 Å |
| 9PL1 Structure of metastable folding intermediate of V17A/V26A ubiquitin derived from pressure-jump NMR data Deposited 2025-07-15 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–76（76 aa）
|
Mutation：V17A, V26A | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.4；298 K；Ionic strength (raw mmCIF value) 25；Pressure 1
NMR sample composition
200 uM [U-100% 13C; U-100% 15N; U-80% 2H] Ubq V17A/V26A, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
200 uM [U-100% 15N] Ubq V17A/V26A, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 9QLO NMT1-NAC bound human RNC with full length ARF1 - State 1 Deposited 2025-03-21 | Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric（88） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 191 G3D GUANOSINE-3'-MONOPHOSPHATE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.47 Å |
| 9QLP NMT1-NAC bound human RNC with full length ARF1 - State 2 Deposited 2025-03-21 | Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric（88） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 191 G3D GUANOSINE-3'-MONOPHOSPHATE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.75 Å |
| 9QLQ NMT1-NAC bound human RNC with full length ARF1 - alternative State Deposited 2025-03-21 | Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric（88） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 191 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.57 Å |
| 9RPV Structure of the ZAK-bound human disome Deposited 2025-06-25 | Assembly 1 Protein–RNA Heteromer；Protein × 154 PDB declaration: 168-meric（168） Consistent with all polymers |
Chain Lm
1–128（128 aa）
Chain Mm
1–128（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 830 ZN ZINC ION × 7 ANM ANISOMYCIN × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.35 Å |
| 9RU7 WT-HEK 80S ribosome bound to Kozak mRNA (WT-Kozak) Deposited 2025-07-03 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | K POTASSIUM ION × 17 MG MAGNESIUM ION × 74 C CYTIDINE-5'-MONOPHOSPHATE × 2 NA SODIUM ION × 7 ZN ZINC ION × 5 HYG HYGROMYCIN B × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9RU8 WT-HEK 80S ribosome bound to TISU mRNA (WT-TISU) Deposited 2025-07-03 | Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 80-meric（80） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 3.00 Å |
| 9RU9 RPS26dC HEK mutant 80S ribosome bound to Kozak mRNA (RPS26dC-Kozak) Deposited 2025-07-03 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric（82） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 2.97 Å |
| 9RUA RPS26dC HEK mutant 80S ribosome bound to TISU mRNA (RPS26dC-TISU) Deposited 2025-07-03 | Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 80-meric（80） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 2.90 Å |
| 9RUC WT-HEK 80S ribosome bound to H2B mRNA (WT-H2B) Deposited 2025-07-03 | Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 80-meric（80） Consistent with all polymers |
Chain Lm
1–127（127 aa）
|
Not recorded | MG MAGNESIUM ION × 223 K POTASSIUM ION × 51 NA SODIUM ION × 8 ZN ZINC ION × 6 HYG HYGROMYCIN B × 1 SPD SPERMIDINE × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 9S3B NMT1-NAC bound human RNC with 58 amino acid ARF1-linker - State 1 Deposited 2025-07-24 | Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric（88） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 191 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.38 Å |
| 9S3C NMT1-NAC bound human RNC with 58 amino acid ARF1-linker - State 2 Deposited 2025-07-24 | Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric（88） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 191 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.42 Å |
| 9S3D NAC bound human RNC with 58 amino acid ARF1-linker Deposited 2025-07-24 | Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric（87） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Not recorded | MG MAGNESIUM ION × 191 ZN ZINC ION × 8 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.32 Å |
| 9SPF CRYO-EM STRUCTURE OF HUMAN 80S RIBOSOME WITH A/P/E-SITE TRNA AND MRNA CONTAINING N1-METHYLPSEUDOURIDINE Deposited 2025-09-17 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | K POTASSIUM ION × 151 MG MAGNESIUM ION × 225 NA SODIUM ION × 35 SPD SPERMIDINE × 19 PUT 1,4-DIAMINOBUTANE × 9 ZN ZINC ION × 7 HYG HYGROMYCIN B × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 9SPI CRYO-EM STRUCTURE OF HUMAN 80S RIBOSOME WITH A/P/E-SITE TRNA AND MRNA CONTAINING URIDINE Deposited 2025-09-17 | Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric（83） Consistent with all polymers |
Chain Lm
1–128（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PUT 1,4-DIAMINOBUTANE × 8 SPD SPERMIDINE × 18 MG MAGNESIUM ION × 195 K POTASSIUM ION × 75 ZN ZINC ION × 7 HYG HYGROMYCIN B × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |