Current Protein Identity：P02554
New Search
Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1FFX TUBULIN:STATHMIN-LIKE DOMAIN COMPLEX Deposited 2000-07-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293.K
|
Resolution 3.95 Å R-free 0.367 |
| 1IA0 KIF1A HEAD-MICROTUBULE COMPLEX STRUCTURE IN ATP-FORM Deposited 2001-03-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TXL TAXOTERE × 1 MG MAGNESIUM ION × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
buf1；pH 7.4；IMIDAZOLE 50 MM, MG-ACETATE 5 MM, EGTA 1 MM, K-ACETATE 50 MM, DTT 10 MM, PACLITAXEL 10 MICRO M
cryo-EM vitrification conditions
PLUNGED INTO ETHANE
X-ray crystallization conditions
polymerization of the microtubule；pH 6.8；296 K；pH 6.8, polymerization of the microtubule, temperature 296K
|
Resolution 15.00 Å |
| 1SA0 TUBULIN-COLCHICINE: STATHMIN-LIKE DOMAIN COMPLEX Deposited 2004-02-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；PEG, PIPES BUFFER, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 3.58 Å R-free 0.249 |
| 1SA1 Tubulin-podophyllotoxin: stathmin-like domain complex Deposited 2004-02-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain E
49–189（141 aa）
|
Not recorded | MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 POD 9-HYDROXY-5-(3,4,5-TRIMETHOXYPHENYL)-5,8,8A,9-TETRAHYDROFURO[3',4':6,7]NAPHTHO[2,3-D][1,3]DIOXOL-6(5AH)-ONE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；PEG, PIPES BUFFER, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 4.20 Å R-free 0.259 |
| 1TUB TUBULIN ALPHA-BETA DIMER, ELECTRON DIFFRACTION Deposited 1997-09-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–427（427 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TXL TAXOTERE × 1 | ELECTRON CRYSTALLOGRAPHY |
X-ray crystallization conditions
TWO-DIMENSIONAL SHEETS ARE FORMED UNDER NORMAL POLYMERIZING CONDITIONS WITH THE ADDITION OF 1 MM ZNCL2
|
Resolution 3.70 Å |
| 1TVK The binding mode of epothilone A on a,b-tubulin by electron crystallography Deposited 2004-06-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–427（427 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 EP EPOTHILONE A × 1 | ELECTRON CRYSTALLOGRAPHY | mmCIF provides none of the parsed conditions | Resolution 2.89 Å R-free 0.321 |
| 2HXF KIF1A head-microtubule complex structure in amppnp-form Deposited 2006-08-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
IMIDAZOLE；pH 7.4；IMIDAZOLE
cryo-EM vitrification conditions
Ethan slash
|
Resolution 10.00 Å |
| 2HXH KIF1A head-microtubule complex structure in adp-form Deposited 2006-08-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
imidazole；pH 7.4；imidazole
cryo-EM vitrification conditions
Ethan slash
|
Resolution 11.00 Å |
| 2WBE Kinesin-5-Tubulin Complex with AMPPNP Deposited 2009-02-26 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
80MM PIPES, 150MM NACL, 7MM MGCL2, 1MM EGTA, 1MM BETA-MERCAPTOETHANOL；pH 6.8；80MM PIPES, 150MM NACL, 7MM MGCL2, 1MM EGTA, 1MM BETA-MERCAPTOETHANOL
cryo-EM vitrification conditions
ETHANE
|
Resolution 9.40 Å |
| 3EDL Kinesin13-Microtubule Ring complex Deposited 2008-09-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 165 PDB declaration: 165-meric（165） Consistent with protein count |
Chain B
1–445（445 aa）
Chain G
1–445（445 aa）
|
Not recorded | ZN ZINC ION × 33 MG MAGNESIUM ION × 99 GTP GUANOSINE-5'-TRIPHOSPHATE × 66 GDP GUANOSINE-5'-DIPHOSPHATE × 66 TA1 TAXOL × 33 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 33 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 33 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；80 mM Pipes, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 2 mM AMP-PNP, 0.02 mM taxol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 28.00 Å |
| 3EDL Kinesin13-Microtubule Ring complex Deposited 2008-09-03 | Assembly 2 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain B
1–445（445 aa）
Chain G
1–445（445 aa）
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；80 mM Pipes, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 2 mM AMP-PNP, 0.02 mM taxol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 28.00 Å |
| 3EDL Kinesin13-Microtubule Ring complex Deposited 2008-09-03 | Assembly 3 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain B
1–445（445 aa）
Chain G
1–445（445 aa）
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 CN2 2-MERCAPTO-N-[1,2,3,10-TETRAMETHOXY-9-OXO-5,6,7,9-TETRAHYDRO-BENZO[A]HEPTALEN-7-YL]ACETAMIDE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；80 mM Pipes, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 2 mM AMP-PNP, 0.02 mM taxol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 28.00 Å |
| 3J6E Energy minimized average structure of Microtubules stabilized by GmpCpp Deposited 2014-02-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain B
1–427（427 aa）
Chain D
1–427（427 aa）
Chain F
1–427（427 aa）
Chain H
1–427（427 aa）
Chain J
1–427（427 aa）
Chain L
1–427（427 aa）
Chain N
1–427（427 aa）
Chain P
1–427（427 aa）
Chain R
1–427（427 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 18 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions
The grid was blotted for 2 seconds before plunging.；90.4 K；Cryogen ETHANE；The grid was blotted for 2 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
|
Resolution 4.70 Å |
| 3J6F Minimized average structure of GDP-bound dynamic microtubules Deposited 2014-02-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain B
1–427（427 aa）
Chain D
1–427（427 aa）
Chain F
1–427（427 aa）
Chain H
1–427（427 aa）
Chain J
1–427（427 aa）
Chain L
1–427（427 aa）
Chain N
1–427（427 aa）
Chain P
1–427（427 aa）
Chain R
1–427（427 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions
The grid was blotted for 2 seconds before plunging.；90.4 K；Cryogen ETHANE；The grid was blotted for 2 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
|
Resolution 4.90 Å |
| 3J6G Minimized average structure of microtubules stabilized by taxol Deposited 2014-02-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain B
1–427（427 aa）
Chain D
1–427（427 aa）
Chain F
1–427（427 aa）
Chain H
1–427（427 aa）
Chain J
1–427（427 aa）
Chain L
1–427（427 aa）
Chain N
1–427（427 aa）
Chain P
1–427（427 aa）
Chain R
1–427（427 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 TA1 TAXOL × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions
The grid was blotted for 2 seconds before plunging.；90.4 K；Cryogen ETHANE；The grid was blotted for 2 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
|
Resolution 5.50 Å |
| 3J6H Nucleotide-free Kinesin motor domain complexed with GMPCPP-microtubule Deposited 2014-02-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
2–427（426 aa）
Fragment：UNP residues 2-427
|
Not recorded | MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 SO4 SULFATE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.10 Å |
| 3J6P Pseudo-atomic model of dynein microtubule binding domain-tubulin complex based on a cryoEM map Deposited 2014-03-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Blot for 3.5 seconds before plunging；Cryogen ETHANE
|
Resolution 8.20 Å |
| 3J7I Structure of alpha- and beta- tubulin in GMPCPP-microtubules Deposited 2014-07-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.90 Å |
| 3JAK Cryo-EM structure of GTPgammaS-microtubule co-polymerized with EB3 (merged dataset with and without kinesin bound) Deposited 2015-06-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain F
1–445（445 aa）
Chain G
1–445（445 aa）
Chain H
1–445（445 aa）
Chain I
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions
Blot once for 4 seconds before plunging.；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
|
Resolution 3.30 Å |
| 3JAL Cryo-EM structure of GMPCPP-microtubule co-polymerized with EB3 Deposited 2015-06-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain F
1–445（445 aa）
Chain G
1–445（445 aa）
Chain H
1–445（445 aa）
Chain I
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions
Blot once for 4 seconds before plunging；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
|
Resolution 3.50 Å |
| 3JAR Cryo-EM structure of GDP-microtubule co-polymerized with EB3 Deposited 2015-06-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain F
1–445（445 aa）
Chain G
1–445（445 aa）
Chain H
1–445（445 aa）
Chain I
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions
Blot once for 4 seconds before plunging/；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
|
Resolution 3.40 Å |
| 3JAS Cryo-EM structure of dynamic GDP-microtubule (14 protofilaments) decorated with kinesin Deposited 2015-06-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain F
1–445（445 aa）
Chain G
1–445（445 aa）
Chain H
1–445（445 aa）
Chain I
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions
Blot once for 4 seconds before plunging.；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
|
Resolution 3.50 Å |
| 3JAT Cryo-EM structure of GMPCPP-microtubule (14 protofilaments) decorated with kinesin Deposited 2015-06-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain F
1–445（445 aa）
Chain G
1–445（445 aa）
Chain H
1–445（445 aa）
Chain I
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 12 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions
Blot once for 4 seconds before plunging.；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
|
Resolution 3.50 Å |
| 3JAW Atomic model of a microtubule seam based on a cryo-EM reconstruction of the EB3-bound microtubule (merged dataset containing tubulin bound to GTPgammaS, GMPCPP, and GDP) Deposited 2015-06-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
BRB80；pH 6.8；80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM vitrification conditions
Blot once for 4 seconds before plunging.；90.4 K；Cryogen ETHANE；Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
|
Resolution 3.90 Å |
| 4ZHQ Crystal structure of Tubulin-Stathmin-TTL-MMAE Complex Deposited 2015-04-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 GOL GLYCEROL × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 4Q5 N-methyl-L-valyl-N-[(3R,4S,5S)-1-{(2S)-2-[(1R,2R)-3-{[(1S,2R)-1-hydroxy-1-phenylpropan-2-yl]amino}-1-methoxy-2-methyl-3-oxopropyl]pyrrolidin-1-yl}-3-methoxy-5-methyl-1-oxoheptan-4-yl]-N-methyl-L-valinamide × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.7；293 K；6% PEG, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2
|
Resolution 2.55 Å R-free 0.245 |
| 4ZI7 CRYSTAL STRUCTURE OF TUBULIN-STATHMIN-TTL-HTI286 COMPLEX Deposited 2015-04-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 4 GOL GLYCEROL × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 4SL N,beta,beta-trimethyl-L-phenylalanyl-N-[(3S,4Z)-5-carboxy-2-methylhex-4-en-3-yl]-N,3-dimethyl-L-valinamide × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；PEG, glycerol, MES, CaCl2, MgCl2
|
Resolution 2.51 Å R-free 0.239 |
| 4ZOL Crystal Structure of Tubulin-Stathmin-TTL-Tubulysin M Complex Deposited 2015-05-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 55Q (2R,4R)-4-{[(2-{(1R,3R)-1-(acetyloxy)-4-methyl-3-[methyl(N-{[(2S)-1-methylpiperidin-2-yl]carbonyl}-D-isoleucyl)amino]pentyl}-1,3-thiazol-4-yl)carbonyl]amino}-2-methyl-5-phenylpentanoic acid × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.7；293 K；6% PEG, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2
|
Resolution 2.50 Å R-free 0.232 |
| 5BMV CRYSTAL STRUCTURE OF TUBULIN-STATHMIN-TTL-Vinblastine COMPLEX Deposited 2015-05-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 GOL GLYCEROL × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 VLB (2ALPHA,2'BETA,3BETA,4ALPHA,5BETA)-VINCALEUKOBLASTINE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；6% PEG, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2
|
Resolution 2.50 Å R-free 0.235 |
| 5FNV a new complex structure of tubulin with an alpha-beta unsaturated lactone Deposited 2015-11-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X3H PIRONETIN × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.7；0.1M MES PH6.7;6% PEG4000 ;5% GLYCEROL;30MM CACL2;30MM MGCL2
|
Resolution 2.61 Å R-free 0.226 |
| 5JQG An apo tubulin-RB-TTL complex structure used for side-by-side comparison Deposited 2016-05-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 CL CHLORIDE ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.7；293 K；0.1M MES, 6% PEG4000, 5% Glycerol, 30mM CaCl2, 30mM MgCl2
|
Resolution 2.24 Å R-free 0.212 |
| 5KMG Near-atomic cryo-EM structure of PRC1 bound to the microtubule Deposited 2016-06-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric（42） Consistent with protein count |
Chain B
1–431（431 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 14 MG MAGNESIUM ION × 14 GDP GUANOSINE-5'-DIPHOSPHATE × 14 POU Peloruside A × 14 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；blot for 4 seconds, blot force 10
|
Resolution 3.50 Å |
| 5KMG Near-atomic cryo-EM structure of PRC1 bound to the microtubule Deposited 2016-06-27 | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–431（431 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 POU Peloruside A × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；blot for 4 seconds, blot force 10
|
Resolution 3.50 Å |
| 5KMG Near-atomic cryo-EM structure of PRC1 bound to the microtubule Deposited 2016-06-27 | Assembly 3 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–431（431 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 POU Peloruside A × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；blot for 4 seconds, blot force 10
|
Resolution 3.50 Å |
| 5MM4 Ustilago maydis kinesin-5 motor domain in the AMPPNP state bound to microtubules Deposited 2016-12-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–427（427 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 TA1 TAXOL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 5MM7 Ustilago maydis kinesin-5 motor domain with N-terminal extension in the AMPPNP state bound to microtubules Deposited 2016-12-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–427（427 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 TA1 TAXOL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.10 Å |
| 5OAM Molecular basis of human kinesin-8 function and inhibition Deposited 2017-06-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.50 Å |
| 5OCU Molecular basis of human kinesin-8 function and inhibition Deposited 2017-07-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.20 Å |
| 5OGC Molecular basis of human kinesin-8 function and inhibition Deposited 2017-07-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | 9V5 4-chloranyl-2-nitro-1-(phenylsulfonyl)benzene × 1 ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å |
| 5SYC Near-atomic resolution cryo-EM reconstruction of peloruside-stabilized microtubule Deposited 2016-08-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 52 PDB declaration: 52-meric（52） Consistent with protein count |
Chain B
1–426（426 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 26 MG MAGNESIUM ION × 26 GDP GUANOSINE-5'-DIPHOSPHATE × 26 POU Peloruside A × 26 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.50 Å |
| 5SYC Near-atomic resolution cryo-EM reconstruction of peloruside-stabilized microtubule Deposited 2016-08-10 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–426（426 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 POU Peloruside A × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.50 Å |
| 5SYC Near-atomic resolution cryo-EM reconstruction of peloruside-stabilized microtubule Deposited 2016-08-10 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–426（426 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 POU Peloruside A × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.50 Å |
| 5SYE Near-atomic resolution cryo-EM reconstruction of doubly bound Taxol- and peloruside-stabilized microtubule Deposited 2016-08-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 52 PDB declaration: 156-meric（156） Review required |
Chain B
1–426（426 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 26 MG MAGNESIUM ION × 26 GDP GUANOSINE-5'-DIPHOSPHATE × 26 TA1 TAXOL × 26 POU Peloruside A × 26 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.50 Å |
| 5SYE Near-atomic resolution cryo-EM reconstruction of doubly bound Taxol- and peloruside-stabilized microtubule Deposited 2016-08-10 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: hexameric（6） Review required |
Chain B
1–426（426 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 POU Peloruside A × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.50 Å |
| 5SYE Near-atomic resolution cryo-EM reconstruction of doubly bound Taxol- and peloruside-stabilized microtubule Deposited 2016-08-10 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: hexameric（6） Review required |
Chain B
1–426（426 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 POU Peloruside A × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.50 Å |
| 5SYF High-resolution cryo-EM reconstruction of Taxol-stabilized microtubule Deposited 2016-08-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 52 PDB declaration: 130-meric（130） Review required |
Chain B
1–426（426 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 26 MG MAGNESIUM ION × 26 GDP GUANOSINE-5'-DIPHOSPHATE × 26 TA1 TAXOL × 26 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.50 Å |
| 5SYF High-resolution cryo-EM reconstruction of Taxol-stabilized microtubule Deposited 2016-08-11 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: pentameric（5） Review required |
Chain B
1–426（426 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.50 Å |
| 5SYF High-resolution cryo-EM reconstruction of Taxol-stabilized microtubule Deposited 2016-08-11 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: pentameric（5） Review required |
Chain B
1–426（426 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.50 Å |
| 5SYG Cryo-EM reconstruction of zampanolide-bound microtubule Deposited 2016-08-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 52 PDB declaration: 130-meric（130） Review required |
Chain B
1–426（426 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 26 MG MAGNESIUM ION × 26 GDP GUANOSINE-5'-DIPHOSPHATE × 26 ZPN (2Z,4E)-N-[(S)-[(1S,2E,5S,8E,10Z,17S)-3,11-dimethyl-19-methylidene-7,13-dioxo-6,21-dioxabicyclo[15.3.1]henicosa-2,8,10-trien-5-yl](hydroxy)methyl]hexa-2,4-dienamide × 26 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.50 Å |
| 5SYG Cryo-EM reconstruction of zampanolide-bound microtubule Deposited 2016-08-11 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: pentameric（5） Review required |
Chain B
1–426（426 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ZPN (2Z,4E)-N-[(S)-[(1S,2E,5S,8E,10Z,17S)-3,11-dimethyl-19-methylidene-7,13-dioxo-6,21-dioxabicyclo[15.3.1]henicosa-2,8,10-trien-5-yl](hydroxy)methyl]hexa-2,4-dienamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.50 Å |
| 5SYG Cryo-EM reconstruction of zampanolide-bound microtubule Deposited 2016-08-11 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: pentameric（5） Review required |
Chain B
1–426（426 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ZPN (2Z,4E)-N-[(S)-[(1S,2E,5S,8E,10Z,17S)-3,11-dimethyl-19-methylidene-7,13-dioxo-6,21-dioxabicyclo[15.3.1]henicosa-2,8,10-trien-5-yl](hydroxy)methyl]hexa-2,4-dienamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds, blot force 10, before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 3.50 Å |
| 5XXT GDP-microtubule complexed with nucleotide-free KIF5C Deposited 2017-07-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain B
2–427（426 aa）
Fragment：UNP residues 2-427
Chain D
2–427（426 aa）
Fragment：UNP residues 2-427
Chain F
2–427（426 aa）
Fragment：UNP residues 2-427
Chain H
2–427（426 aa）
Fragment：UNP residues 2-427
Chain J
2–427（426 aa）
Fragment：UNP residues 2-427
Chain L
2–427（426 aa）
Fragment：UNP residues 2-427
Chain N
2–427（426 aa）
Fragment：UNP residues 2-427
Chain P
2–427（426 aa）
Fragment：UNP residues 2-427
Chain R
2–427（426 aa）
Fragment：UNP residues 2-427
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.35 Å |
| 5XXV GDP-microtubule complexed with KIF5C in AMPPNP state Deposited 2017-07-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain B
2–427（426 aa）
Fragment：UNP residues 2-427
Chain D
2–427（426 aa）
Fragment：UNP residues 2-427
Chain F
2–427（426 aa）
Fragment：UNP residues 2-427
Chain H
2–427（426 aa）
Fragment：UNP residues 2-427
Chain J
2–427（426 aa）
Fragment：UNP residues 2-427
Chain L
2–427（426 aa）
Fragment：UNP residues 2-427
Chain N
2–427（426 aa）
Fragment：UNP residues 2-427
Chain P
2–427（426 aa）
Fragment：UNP residues 2-427
Chain R
2–427（426 aa）
Fragment：UNP residues 2-427
|
Not recorded | MG MAGNESIUM ION × 9 GTP GUANOSINE-5'-TRIPHOSPHATE × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.46 Å |
| 5XXW GDP-microtubule complexed with KIF5C in ATP state Deposited 2017-07-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain B
2–427（426 aa）
Fragment：UNP residues 2-427
Chain D
2–427（426 aa）
Fragment：UNP residues 2-427
Chain F
2–427（426 aa）
Fragment：UNP residues 2-427
Chain H
2–427（426 aa）
Fragment：UNP residues 2-427
Chain J
2–427（426 aa）
Fragment：UNP residues 2-427
Chain L
2–427（426 aa）
Fragment：UNP residues 2-427
Chain N
2–427（426 aa）
Fragment：UNP residues 2-427
Chain P
2–427（426 aa）
Fragment：UNP residues 2-427
Chain R
2–427（426 aa）
Fragment：UNP residues 2-427
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 9 GDP GUANOSINE-5'-DIPHOSPHATE × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.00 Å |
| 5XXX GMPCPP-microtubule complexed with nucleotide-free KIF5C Deposited 2017-07-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric（18） Consistent with protein count |
Chain B
2–427（426 aa）
Fragment：UNP residues 2-427
Chain D
2–427（426 aa）
Fragment：UNP residues 2-427
Chain F
2–427（426 aa）
Fragment：UNP residues 2-427
Chain H
2–427（426 aa）
Fragment：UNP residues 2-427
Chain J
2–427（426 aa）
Fragment：UNP residues 2-427
Chain L
2–427（426 aa）
Fragment：UNP residues 2-427
Chain N
2–427（426 aa）
Fragment：UNP residues 2-427
Chain P
2–427（426 aa）
Fragment：UNP residues 2-427
Chain R
2–427（426 aa）
Fragment：UNP residues 2-427
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 18 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.43 Å |
| 6BJC TPX2_mini decorated GMPCPP-microtubule Deposited 2017-11-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain F
1–445（445 aa）
Chain G
1–445（445 aa）
Chain H
1–445（445 aa）
Chain I
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 12 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；BRB80
cryo-EM vitrification conditions
Cryogen ETHANE；blot for 4 seconds before plunging
|
Resolution 3.30 Å |
| 6CVJ Model of synthetic tau (four tandem repeats of first repeat sequence) bound to the microtubule Deposited 2018-03-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 120 PDB declaration: 120-meric（120） Consistent with protein count |
Chain B
1–445（445 aa）
Chain C
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 30 MG MAGNESIUM ION × 30 GDP GUANOSINE-5'-DIPHOSPHATE × 60 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash
|
Resolution 3.20 Å |
| 6CVJ Model of synthetic tau (four tandem repeats of first repeat sequence) bound to the microtubule Deposited 2018-03-28 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
1–445（445 aa）
Chain C
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash
|
Resolution 3.20 Å |
| 6CVJ Model of synthetic tau (four tandem repeats of first repeat sequence) bound to the microtubule Deposited 2018-03-28 | Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
1–445（445 aa）
Chain C
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash
|
Resolution 3.20 Å |
| 6CVN Model of synthetic tau (R2x4) bound to the microtubule Deposited 2018-03-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 120 PDB declaration: 120-meric（120） Consistent with protein count |
Chain A
1–445（445 aa）
Chain C
1–445（445 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 60 GTP GUANOSINE-5'-TRIPHOSPHATE × 30 MG MAGNESIUM ION × 30 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash.
|
Resolution 3.90 Å |
| 6CVN Model of synthetic tau (R2x4) bound to the microtubule Deposited 2018-03-28 | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–445（445 aa）
Chain C
1–445（445 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash.
|
Resolution 3.90 Å |
| 6CVN Model of synthetic tau (R2x4) bound to the microtubule Deposited 2018-03-28 | Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–445（445 aa）
Chain C
1–445（445 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；blot force 10 pN, 6 second blot time. used C-flat 1.2/1.3 holey grids. First 2 uL of microtubules were adhered to grid for 30 seconds, followed by 2 4 uL washes of tau with 30 second incubation for each wash.
|
Resolution 3.90 Å |
| 6DPU Undecorated GMPCPP microtubule Deposited 2018-06-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain F
1–445（445 aa）
Chain G
1–445（445 aa）
Chain H
1–445（445 aa）
Chain I
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 12 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；BRB80 buffer (80 mM PIPES, pH 6.8, 1 mM EGTA, 1 mM MgCl2) supplemented with 1 mM GMPCPP, 1 mM DTT, and 0.05% Nonident P-40
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging.
|
Resolution 3.10 Å |
| 6DPV Undecorated GDP microtubule Deposited 2018-06-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain F
1–445（445 aa）
Chain G
1–445（445 aa）
Chain H
1–445（445 aa）
Chain I
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；BRB80 buffer (80 mM PIPES pH 6.8, 1 mM EGTA, 1 mM MgCl2) supplemented with 1 mM GTP, 1 mM DTT, and 0.05% Nonident P-40
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging.
|
Resolution 3.30 Å |
| 6DPW Undecorated GTPgammaS microtubule Deposited 2018-06-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain F
1–445（445 aa）
Chain G
1–445（445 aa）
Chain H
1–445（445 aa）
Chain I
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；Modified BRB80 buffer (80 mM PIPES, pH 6.8, 1 mM EGTA, 0.2 mM MgCl2) supplemented with 1 mM GTPgammaS, 1 mM DTT, and 0.05% Nonident P-40
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 4 seconds before plunging.
|
Resolution 3.50 Å |
| 6EVW Cryo-EM structure of GMPCPP-microtubule co-polymerised with doublecortin Deposited 2017-11-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain B
1–429（429 aa）
Chain D
1–429（429 aa）
Chain F
1–429（429 aa）
Chain G
1–429（429 aa）
Chain H
1–429（429 aa）
Chain I
1–429（429 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 12 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å |
| 6EVX Cryo-EM structure of GDP.Pi-microtubule rapidly co-polymerised with doublecortin Deposited 2017-11-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain F
1–445（445 aa）
Chain G
1–445（445 aa）
Chain H
1–445（445 aa）
Chain I
1–445（445 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 PO4 PHOSPHATE ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6EVY Cryo-EM structure of GTPgammaS-microtubule co-polymerised with doublecortin Deposited 2017-11-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain F
1–445（445 aa）
Chain G
1–445（445 aa）
Chain H
1–445（445 aa）
Chain I
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å |
| 6EVZ Cryo-EM structure of GDP-microtubule co-polymerised with doublecortin Deposited 2017-11-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain F
1–445（445 aa）
Chain G
1–445（445 aa）
Chain H
1–445（445 aa）
Chain I
1–445（445 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6EW0 Cryo-EM structure of GDP-microtubule co-polymerised with doublecortin and supplemented with Taxol Deposited 2017-11-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain F
1–445（445 aa）
Chain G
1–445（445 aa）
Chain H
1–445（445 aa）
Chain I
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 TA1 TAXOL × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6KIO Complex of yeast cytoplasmic dynein MTBD-High and MT without DTT Deposited 2019-07-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain b
2–427（426 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.94 Å |
| 6KIQ Complex of yeast cytoplasmic dynein MTBD-High and MT with DTT Deposited 2019-07-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain b
2–427（426 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；DTT was added to the final concentration of 1 mM.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.62 Å |
| 6LS4 A novel anti-tumor agent S-40 in complex with tubulin Deposited 2020-01-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 GOL GLYCEROL × 1 S40 3-[(4-cyclopropylphenyl)sulfonylamino]-4-methyl-N-(pyridin-3-ylmethyl)benzamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；6% PEG 4000, 6% GLYCEROL, 30mM MAGNESIUM CHLORIDE, 30mM CALCIUM CHLORIDE, 3.3% Jeffamin M-600, 100mM MES
|
Resolution 2.40 Å R-free 0.240 |
| 6MLQ Cryo-EM structure of microtubule-bound Kif7 in the ADP state Deposited 2018-09-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted from behind the grid for 2 seconds
|
Resolution 4.20 Å |
| 6MLR Cryo-EM structure of microtubule-bound Kif7 in the AMPPNP state Deposited 2018-09-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted from behind the grid for 2 seconds
|
Resolution 4.20 Å |
| 6MZE Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain I
1–445（445 aa）
Chain K
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
|
Resolution 3.60 Å R-free 0.245 |
| 6MZE Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 | Assembly 2 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count |
Chain P
1–445（445 aa）
Chain R
1–445（445 aa）
Chain W
1–445（445 aa）
Chain Y
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
|
Resolution 3.60 Å R-free 0.245 |
| 6MZF Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain I
1–445（445 aa）
Chain K
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
|
Resolution 4.40 Å R-free 0.252 |
| 6MZF Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 | Assembly 2 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric（14） Consistent with protein count |
Chain P
1–445（445 aa）
Chain R
1–445（445 aa）
Chain W
1–445（445 aa）
Chain Y
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
|
Resolution 4.40 Å R-free 0.252 |
| 6MZG Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
|
Resolution 3.21 Å R-free 0.234 |
| 6MZG Structural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays Deposited 2018-11-05 | Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain H
1–445（445 aa）
Chain J
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000
|
Resolution 3.21 Å R-free 0.234 |
| 6O2Q Acetylated Microtubules Deposited 2019-02-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain F
1–445（445 aa）
Chain G
1–445（445 aa）
Chain H
1–445（445 aa）
Chain I
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；Contains 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH (stored at 4 degrees Celsius).
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 4 seconds at blot force 10.
|
Resolution 3.70 Å |
| 6O2R Deacetylated Microtubules Deposited 2019-02-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain F
1–445（445 aa）
Chain G
1–445（445 aa）
Chain H
1–445（445 aa）
Chain I
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；Contains 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH (stored at 4 degrees Celsius).
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 4 seconds at blot force 10.
|
Resolution 3.30 Å |
| 6O2S Deacetylated Microtubules Deposited 2019-02-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 104 PDB declaration: 104-meric（104） Consistent with protein count |
Chain 1H
1–445（445 aa）
Chain 1O
1–445（445 aa）
Chain 1P
1–445（445 aa）
Chain 1Q
1–445（445 aa）
Chain 1R
1–445（445 aa）
Chain 1S
1–445（445 aa）
Chain 1T
1–445（445 aa）
Chain 1U
1–445（445 aa）
Chain 1V
1–445（445 aa）
Chain 1W
1–445（445 aa）
Chain 1X
1–445（445 aa）
Chain 1Y
1–445（445 aa）
Chain 1Z
1–445（445 aa）
Chain 2H
1–445（445 aa）
Chain 2O
1–445（445 aa）
Chain 2P
1–445（445 aa）
Chain 2Q
1–445（445 aa）
Chain 2R
1–445（445 aa）
Chain 2S
1–445（445 aa）
Chain 2T
1–445（445 aa）
Chain 2U
1–445（445 aa）
Chain 2V
1–445（445 aa）
Chain 2W
1–445（445 aa）
Chain 2X
1–445（445 aa）
Chain 2Y
1–445（445 aa）
Chain 2Z
1–445（445 aa）
Chain 3H
1–445（445 aa）
Chain 3O
1–445（445 aa）
Chain 3P
1–445（445 aa）
Chain 3Q
1–445（445 aa）
Chain 3R
1–445（445 aa）
Chain 3S
1–445（445 aa）
Chain 3T
1–445（445 aa）
Chain 3U
1–445（445 aa）
Chain 3V
1–445（445 aa）
Chain 3W
1–445（445 aa）
Chain 3X
1–445（445 aa）
Chain 3Y
1–445（445 aa）
Chain 3Z
1–445（445 aa）
Chain 4H
1–445（445 aa）
Chain 4O
1–445（445 aa）
Chain 4P
1–445（445 aa）
Chain 4Q
1–445（445 aa）
Chain 4R
1–445（445 aa）
Chain 4S
1–445（445 aa）
Chain 4T
1–445（445 aa）
Chain 4U
1–445（445 aa）
Chain 4V
1–445（445 aa）
Chain 4W
1–445（445 aa）
Chain 4X
1–445（445 aa）
Chain 4Y
1–445（445 aa）
Chain 4Z
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 52 MG MAGNESIUM ION × 52 GDP GUANOSINE-5'-DIPHOSPHATE × 52 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；Contains 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH (stored at 4 degrees Celsius).
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 4 seconds at blot force 10.
|
Resolution 4.00 Å |
| 6O2T Acetylated Microtubules Deposited 2019-02-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 104 PDB declaration: 104-meric（104） Consistent with protein count |
Chain 1H
1–445（445 aa）
Chain 1O
1–445（445 aa）
Chain 1P
1–445（445 aa）
Chain 1Q
1–445（445 aa）
Chain 1R
1–445（445 aa）
Chain 1S
1–445（445 aa）
Chain 1T
1–445（445 aa）
Chain 1U
1–445（445 aa）
Chain 1V
1–445（445 aa）
Chain 1W
1–445（445 aa）
Chain 1X
1–445（445 aa）
Chain 1Y
1–445（445 aa）
Chain 1Z
1–445（445 aa）
Chain 2H
1–445（445 aa）
Chain 2O
1–445（445 aa）
Chain 2P
1–445（445 aa）
Chain 2Q
1–445（445 aa）
Chain 2R
1–445（445 aa）
Chain 2S
1–445（445 aa）
Chain 2T
1–445（445 aa）
Chain 2U
1–445（445 aa）
Chain 2V
1–445（445 aa）
Chain 2W
1–445（445 aa）
Chain 2X
1–445（445 aa）
Chain 2Y
1–445（445 aa）
Chain 2Z
1–445（445 aa）
Chain 3H
1–445（445 aa）
Chain 3O
1–445（445 aa）
Chain 3P
1–445（445 aa）
Chain 3Q
1–445（445 aa）
Chain 3R
1–445（445 aa）
Chain 3S
1–445（445 aa）
Chain 3T
1–445（445 aa）
Chain 3U
1–445（445 aa）
Chain 3V
1–445（445 aa）
Chain 3W
1–445（445 aa）
Chain 3X
1–445（445 aa）
Chain 3Y
1–445（445 aa）
Chain 3Z
1–445（445 aa）
Chain 4H
1–445（445 aa）
Chain 4O
1–445（445 aa）
Chain 4P
1–445（445 aa）
Chain 4Q
1–445（445 aa）
Chain 4R
1–445（445 aa）
Chain 4S
1–445（445 aa）
Chain 4T
1–445（445 aa）
Chain 4U
1–445（445 aa）
Chain 4V
1–445（445 aa）
Chain 4W
1–445（445 aa）
Chain 4X
1–445（445 aa）
Chain 4Y
1–445（445 aa）
Chain 4Z
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 52 MG MAGNESIUM ION × 52 GDP GUANOSINE-5'-DIPHOSPHATE × 52 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；Contains 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH (stored at 4 degrees Celsius).
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 4 seconds at blot force 10.
|
Resolution 4.10 Å |
| 6RZA Cryo-EM structure of the human inner arm dynein DNAH7 microtubule binding domain bound to microtubules Deposited 2019-06-13 | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain B
1–426（426 aa）
Chain D
1–426（426 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 6RZB Cryo-EM structure of mouse cytoplasmic dynein-1 microtubule binding domain bound to microtubules Deposited 2019-06-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–426（426 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 6TA3 Human kinesin-5 motor domain in the GSK-1 state bound to microtubules (Conformation 1) Deposited 2019-10-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–429（429 aa）
|
Not recorded | G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 MG MAGNESIUM ION × 2 MZK 6-[4-(trifluoromethyl)phenyl]-3,4-dihydro-1~{H}-quinolin-2-one × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6TA4 Human kinesin-5 motor domain in the AMPPNP state bound to microtubules Deposited 2019-10-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–429（429 aa）
|
Not recorded | G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.10 Å |
| 6TIW Human kinesin-5 motor domain in the GSK state bound to microtubules (Conformation 2) Deposited 2019-11-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–429（429 aa）
|
Not recorded | MZK 6-[4-(trifluoromethyl)phenyl]-3,4-dihydro-1~{H}-quinolin-2-one × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6VPO Cryo-EM structure of microtubule-bound KLP61F motor domain in the AMPPNP state Deposited 2020-02-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å |
| 6VPP Cryo-EM structure of microtubule-bound KLP61F motor with tail domain in the nucleotide-free state Deposited 2020-02-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å |
| 6Y4M Structure of Tubulin Tyrosine Ligase in Complex with Tb111 Deposited 2020-02-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 O9B (2~{R})-1-methylpiperidine-2-carboxylic acid × 1 O9H [(1~{R},3~{R})-1-acetyloxy-1-[4-methanoyl-5-(2-phenylmethoxyethyl)-2,3-dihydro-1,3-thiazol-2-yl]-4-methyl-pentan-3-yl]-methyl-azanium × 1 OH5 (2~{S},4~{R})-4-azanyl-2-methyl-5-phenyl-pentanoic acid × 1 PGE TRIETHYLENE GLYCOL × 1 VAL VALINE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.03 M calcium chloride, 0.03 M magnesium chloride, 0.10 M MES pH 6.50, 3.20% w/v
PEG 4000, 5.00% w/v glycerol
|
Resolution 3.34 Å R-free 0.234 |
| 6Y4N Structure of Tubulin Tyrosine Ligase in Complex with Tb116 Deposited 2020-02-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 O9B (2~{R})-1-methylpiperidine-2-carboxylic acid × 1 O9K [(1~{R},3~{R})-1-(4-methanoyl-1,3-thiazol-2-yl)-4-methyl-3-(methylamino)pentyl] ethanoate × 1 O9N methyl (2~{S},4~{S})-2,4-bis(azanyl)-5-phenyl-pentanoate × 1 PGE TRIETHYLENE GLYCOL × 1 VAL VALINE × 1 P6S benzyl hydrogen carbonate × 1 PEG DI(HYDROXYETHYL)ETHER × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；30 mM calcium chloride, 30 mM magnesium chloride, 0.10 M MES pH 6.0, 2.0% w/v PEG 4000, 5.0% w/v glycerol
|
Resolution 2.85 Å R-free 0.220 |
| 6ZPI Microtubule complexed with Kif15 motor domain. Symmetrised asymmetric unit Deposited 2020-07-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–431（431 aa）
|
Not recorded | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 7CBZ Crystal structure of T2R-TTL-A31 complex Deposited 2020-06-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 FUO 2-[5-[4-[2-[4-(2-cyclopropylethanoyl)piperazin-1-yl]ethoxy]phenyl]pyridin-2-yl]-N-(phenylmethyl)ethanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG4000, 8% glycerol, 0.1 M
MES (pH 6.7), 30 mM CaCl2, and 30 mM MgCl2.
|
Resolution 2.61 Å R-free 0.255 |
| 7DMZ GMPCPP microtubule complex Deposited 2020-12-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain C
1–445（445 aa）
Chain E
1–445（445 aa）
Chain F
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 2 TAJ taccalonolide AJ × 3 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 7DN0 AJ-GMPCPP-MT-non-seam Deposited 2020-12-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain E
1–445（445 aa）
Chain F
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 4 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 TAJ taccalonolide AJ × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 7EMJ Crystal structure of T2R-TTL-Barbigerone complex Deposited 2021-04-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 J6L 8,8-dimethyl-3-(2,4,5-trimethoxyphenyl)pyrano[2,3-f]chromen-4-one × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1 M MES, 30 mM CaCl2 and 30 mM MgCl2, pH 6.7
|
Resolution 2.33 Å R-free 0.241 |
| 7EXC Crystal structure of T2R-TTL-1129A2 complex Deposited 2021-05-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 2 GOL GLYCEROL × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 JEL N-[[3-(1,3-benzodioxol-5-yloxy)phenyl]methyl]-9H-pyrido[3,4-b]indol-3-amine × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, 30 mM MgCl2
|
Resolution 2.39 Å R-free 0.264 |
| 7L05 Complex of novel maytansinoid M24 bound to T2R-TTL (two tubulin alpha/beta heterodimers, RB3 stathmin-like domain, and tubulin tyrosine ligase) Deposited 2020-12-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 5 CA CALCIUM ION × 4 CL CHLORIDE ION × 1 EDO 1,2-ETHANEDIOL × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 XQ4 (1S,2R,3S,5S,6S,16E,18E,20R,21S)-11-chloro-21-hydroxy-12,20-dimethoxy-2,5,9,16-tetramethyl-8,23-dioxo-4,24-dioxa-9,22-diazatetracyclo[19.3.1.1~10,14~.0~3,5~]hexacosa-10(26),11,13,16,18-pentaen-6-yl (2S)-2-{methyl[3-(methylamino)propanoyl]amino}propanoate (non-preferred name) × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；PEG 3350, MES, CaCl2, MgCl2, glycerol
|
Resolution 2.21 Å R-free 0.217 |
| 7NB8 Plasmodium falciparum kinesin-5 motor domain without nucleotide, complexed with 14 protofilament microtubule. Deposited 2021-01-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 uM microtubules were incubated to the grid and incubated for 30 seconds. The grid was blotted, then 40 uM kinesin motor domain added and incubated for 30 seconds. The grid was again blotted and 40 uM kinesin motor domain added for 30 seconds. Then the grid was blotted and plunge frozen.
|
Resolution 4.40 Å |
| 7NBA Plasmodium falciparum kinesin-5 motor domain bound to AMPPNP, complexed with 14 protofilament microtubule. Deposited 2021-01-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；5 uM microtubules were applied to the grid and incubated for 30 seconds. The grid was blotted, then 40 uM kinesin motor domain added, incubated for 30 seconds, then blotted. 40 uM Kinesin motor domain was again added, incubated for 40 seconds, blotted, then plunge frozen.
|
Resolution 4.00 Å |
| 7PQC tau-microtubule structural ensemble based on CryoEM data Deposited 2021-09-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric（15） Consistent with protein count |
Chain A
1–445（445 aa）
Chain C
1–445（445 aa）
Chain E
1–445（445 aa）
Chain G
1–445（445 aa）
Chain I
1–445（445 aa）
Chain K
1–445（445 aa）
Chain M
1–445（445 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 7 MG MAGNESIUM ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 7PQP tau-microtubule structural ensemble based on CryoEM data Deposited 2021-09-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric（15） Consistent with protein count |
Chain A
1–445（445 aa）
Chain C
1–445（445 aa）
Chain E
1–445（445 aa）
Chain G
1–445（445 aa）
Chain I
1–445（445 aa）
Chain K
1–445（445 aa）
Chain M
1–445（445 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 7 MG MAGNESIUM ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 7RS5 Cryo-EM structure of Kip3 (AMPPNP) bound to Taxol-Stabilized Microtubules Deposited 2021-08-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric（27） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain F
1–445（445 aa）
Chain H
1–445（445 aa）
Chain J
1–445（445 aa）
Chain M
1–445（445 aa）
Chain O
1–445（445 aa）
Chain Q
1–445（445 aa）
Chain S
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 18 GDP GUANOSINE-5'-DIPHOSPHATE × 9 TA1 TAXOL × 9 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8；cryoEM buffer (50 mM Tris-HCl, pH 8.0, 1 mM MgCl2, 1 mM EGTA, 1 mM DTT supplemented with 2mM AMPPNP)
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 7RS6 Cryo-EM structure of Kip3 (AMPPNP) bound to GMPCPP-Stabilized Microtubules Deposited 2021-08-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric（27） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain F
1–445（445 aa）
Chain H
1–445（445 aa）
Chain J
1–445（445 aa）
Chain M
1–445（445 aa）
Chain O
1–445（445 aa）
Chain Q
1–445（445 aa）
Chain S
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 9 MG MAGNESIUM ION × 27 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；BRB80 buffer (80 mM PIPES-KOH, pH 6.8; 1 mM MgCl2, 1 mM EGTA, 1 mM DTT)
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 7RX0 Complex of AMPPNP-Kif7 and Gli2 Zinc-Finger domain bound to microtubules Deposited 2021-08-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted from behind the grid for 2 seconds
|
Resolution 3.89 Å |
| 7SGS Cryo-EM structure of full-length MAP7 bound to the microtubule Deposited 2021-10-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain C
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 7U0F HIV-1 Rev in complex with tubulin Deposited 2022-02-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 150 PDB declaration: 150-meric（150） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.53 Å |
| 7U0F HIV-1 Rev in complex with tubulin Deposited 2022-02-18 | Assembly 2 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric（10） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.53 Å |
| 7U0F HIV-1 Rev in complex with tubulin Deposited 2022-02-18 | Assembly 3 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric（10） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.53 Å |
| 7X4N Crystal Structure of C. elegans kinesin-4 KLP-12 complexed with tubulin and DARPin Deposited 2022-03-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；ammonium acetate, HEPES, polyethylene glycol (PEG) 3350
|
Resolution 2.88 Å R-free 0.296 |
| 7YHN ANTI-TUMOR AGENT Y48 IN COMPLEX WITH TUBULIN Deposited 2022-07-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 IUK 4-methyl-3-[(4-methylphenyl)sulfonylamino]-~{N}-[(6-methylpyridin-3-yl)methyl]benzamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；6% PEG 4000, 8% GLYCEROL, 30MM MAGNESIUM CHLORIDE, 30MM CALCIUM CHLORIDE, 100MM MES
|
Resolution 2.60 Å R-free 0.272 |
| 7YSN Tubulin heterodimer structure of GMPCPP state in solution Deposited 2022-08-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 7YSO Tubulin heterodimer structure of GDP-1 state in solution Deposited 2022-08-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 7YSP Tubulin heterodimer structure of GDP-2 state in solution Deposited 2022-08-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 7Z2A P. berghei kinesin-8B motor domain in no nucleotide state bound to tubulin dimer Deposited 2022-02-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain H
1–426（426 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 7Z2B P. berghei kinesin-8B motor domain in AMPPNP state bound to tubulin dimer Deposited 2022-02-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain H
1–426（426 aa）
|
Not recorded | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 7Z2C P. falciparum kinesin-8B motor domain in no nucleotide bound to tubulin dimer Deposited 2022-02-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain H
1–426（426 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 8JJB Crystal structure of T2R-TTL-Y61 complex Deposited 2023-05-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–431（431 aa）
Chain D
1–431（431 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 USI ~{N}4-(1~{H}-indol-5-ylmethyl)-6-(3-methoxyphenyl)pyrimidine-2,4-diamine × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 CL CHLORIDE ION × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, 30 mM MgCl2
|
Resolution 2.68 Å R-free 0.260 |
| 8JJC Tubulin-Y62 Deposited 2023-05-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–431（431 aa）
Chain D
1–431（431 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 UPO 4-(6,7-dimethoxy-3,4-dihydro-1~{H}-isoquinolin-2-yl)-6-(3-methoxyphenyl)pyrimidin-2-amine × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 CL CHLORIDE ION × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, 30 mM MgCl2
|
Resolution 2.76 Å R-free 0.264 |
| 8QAU Outer kinetochore Ndc80-Dam1 alpha/beta-tubulin complex Deposited 2023-08-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.54 Å |
| 8RC1 MAP7 MTBD (microtubule binding domain) decorated microtubule protofilament Deposited 2023-12-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain B
1–430（430 aa）
Chain D
1–430（430 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5；80mM PIPES, 2mM MgCl2, 1mM EGTA
cryo-EM vitrification conditions
Cryogen ETHANE；Microtubules stabilised by MAP7 MTBD were applied to a glow discharged grid, incubated for 30 seconds at room temperature following which excess sample was wicked off and MAP7 MTBD was immediately added to the grid. After incubation for 60 seconds in a Vitrobot, excess liquid was blotted off and the grid was plunge frozen in liquid ethane.
|
Resolution 3.70 Å |
| 8RHB Microtubule-associated kinesin-1 tail complex bound to AMPPNP, single-headed state Deposited 2023-12-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；BRB80 + 5mM AMPPNP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8RHH Microtubule-associated kinesin-1 tail complex bound to AMPPNP, two-headed state Deposited 2023-12-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: pentameric（5） Review required |
Chain B
1–445（445 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；BRB80 + 5mM AMPPNP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8RIK Microtubule-associated kinesin-1 tail complex bound to ADP, single-headed state Deposited 2023-12-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: tetrameric（4） Review required |
Chain B
1–445（445 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；BRB80 + 5 mM ADP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8RIZ Microtubule-associated kinesin-1 tail complex bound to ADP, two-headed state Deposited 2023-12-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；BRB80 + 5mM ADP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8V4K CCP5 in complex with microtubules class1 Deposited 2023-11-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | MG MAGNESIUM ION × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ZN ZINC ION × 1 GLU GLUTAMIC ACID × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8V4L CCP5 in complex with microtubules class2 Deposited 2023-11-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: hexameric（6） Review required |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | MG MAGNESIUM ION × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 GLU GLUTAMIC ACID × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8V4M CCP5 in complex with microtubules class3 Deposited 2023-11-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: hexameric（6） Review required |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | MG MAGNESIUM ION × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 GLU GLUTAMIC ACID × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8X9P HURP (428-534)-alpha-tubulin-beta-tubulin complex Deposited 2023-11-30 | Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–427（427 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.54 Å |
| 9DA8 Tau-Microtubule structure in the presence of ATP Deposited 2024-08-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–445（445 aa）
Chain C
1–445（445 aa）
Chain K
1–445（445 aa）
Chain M
1–445（445 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 4 TA1 TAXOL × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.94 Å |
| 9DHZ Cryo-EM structure of HURP bound to a microtubule Deposited 2024-09-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain A
1–445（445 aa）
Chain K
1–445（445 aa）
|
Not recorded | TA1 TAXOL × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9DI0 Cryo-EM structure of Kif18A bound to a microtubule Deposited 2024-09-04 | Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain K
1–445（445 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 2 TA1 TAXOL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9DUQ HURP(65-174) bound to GMPCPP-stabilized microtubule Deposited 2024-10-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric（27） Consistent with protein count |
Chain B
1–427（427 aa）
Chain D
1–427（427 aa）
Chain F
1–427（427 aa）
Chain H
1–427（427 aa）
Chain J
1–427（427 aa）
Chain L
1–427（427 aa）
Chain N
1–427（427 aa）
Chain P
1–427（427 aa）
Chain R
1–427（427 aa）
|
Not recorded | G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 9 MG MAGNESIUM ION × 18 GTP GUANOSINE-5'-TRIPHOSPHATE × 9 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；BRB80 (1X): 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 with KOH
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9DXC Model of tubulin dimers used for determining the dimer rise in a taxol-stabilized microtubule-HURP complex Deposited 2024-10-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9DXE Model of tubulin dimers used for determining the dimer rise in a taxol-stabilized microtubule Deposited 2024-10-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9EDR Tubulin Cofactors D,E,G,C and Tubulin complex -- TBCC N Terminus Bound to Tubulin Deposited 2024-11-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 9EDS Tubulin cofactors D,E,G,C bound to tubulin dimer -- TBCC N terminus unbound Deposited 2024-11-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 9EDT Tubulin cofactors D,E,G bound to tubulin dimer Deposited 2024-11-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 9EEB Tubulin cofactors D,E,G bound to tubulin dimer Deposited 2024-11-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 9GNQ Microtubule-associated Kif5B IAK tail mutant bound to ADP Deposited 2024-09-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；BRB80 + 5mM AMPPNP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9IM5 Tubulin-RB3(MUT)-TTL-Y12 Deposited 2024-07-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 CA CALCIUM ION × 3 CL CHLORIDE ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 A1L2T ~{N}4-(1,3-benzodioxol-5-ylmethyl)-6-(1~{H}-indol-4-yl)pyrimidine-2,4-diamine × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7
|
Resolution 2.86 Å R-free 0.246 |
| 9IMO Crystal structure of Tubulin-RB3-TTL-Y12 Deposited 2024-07-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–431（431 aa）
Chain D
1–431（431 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 5 CA CALCIUM ION × 3 A1L2T ~{N}4-(1,3-benzodioxol-5-ylmethyl)-6-(1~{H}-indol-4-yl)pyrimidine-2,4-diamine × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7
|
Resolution 2.75 Å R-free 0.255 |
| 9KBW CryoEM structure of microtubule bound with GAS2-GAR domain. Deposited 2024-10-31 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–426（426 aa）
|
Not recorded | ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；80 mM PIPES, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 1mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9L7M Nucleotide-free kinesin-1 motor domain bound to the microtubule Deposited 2024-12-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.48 Å |
| 9N9E Cryo-EM structure of the SAH domain of Caenorhabditis elegans ICP-1 bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9N9F Cryo-EM structure of the SAH domain of human INCENP bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.91 Å |
| 9N9G Cryo-EM structure of the human Hec1-Nuf2 dimer bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric（9） Consistent with protein count |
Chain B
1–445（445 aa）
Chain b
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9N9H Cryo-EM structure of the Xenopus laevis mitotic centromere-associated kinesin (MCAK) bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain B
1–445（445 aa）
Chain b
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.31 Å |
| 9N9I Cryo-EM structure of the Xenopus laevis mitotic centromere-associated kinesin (MCAK) bound to the paclitaxel-stabilized microtubule Deposited 2025-02-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric（9） Consistent with protein count |
Chain 3
1–445（445 aa）
Chain B
1–445（445 aa）
Chain C
1–445（445 aa）
Chain b
1–445（445 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 4 TA1 TAXOL × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9NNL Bordetella filamentous hemagglutinin (FhaB) C-terminal domain bound to microtubules Deposited 2025-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
Chain F
1–445（445 aa）
Chain H
1–445（445 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 4 TA1 TAXOL × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.42 Å |
| 9RPD D. melanogaster Augmin TII N-clamp (GST-fusion) bound to a microtubule, well-defined subset of particles Deposited 2025-06-24 | Assembly 1 Insufficient information Heteromer；Protein × 9 PDB declaration: nonameric（9） Consistent with protein count |
Chain B
1–445（445 aa）
Chain D
1–445（445 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.90 Å |
| 9T1D Cryo-EM reconstruction of undecorated GDP microtubule Deposited 2025-10-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–429（429 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.90 Å |
| 9U6A Tubulin-DARPin D1 in complex with a flavone Deposited 2025-03-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–431（431 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 A1L8X 3-methoxy-2-(4-methoxy-3-oxidanyl-phenyl)-5,7-bis(oxidanyl)chromen-4-one × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；PEG 3000, ammonium sulfate, bis-tris methane
|
Resolution 1.92 Å R-free 0.224 |
| 9U6D Crystal structure of tubulin-RB3-TTL in complex with X8 Deposited 2025-03-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–431（431 aa）
Chain D
1–431（431 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 5 CA CALCIUM ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 A1L8Z 2-[2-fluoranyl-4-[4-[2-[4-[(1~{R},2~{S})-2-fluoranylcyclopropyl]carbonylpiperazin-1-yl]ethoxy]phenyl]phenyl]-~{N}-(phenylmethyl)ethanamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7
|
Resolution 2.54 Å R-free 0.238 |
| 9U9Q Tubulin-RB3-TTL in complex with C38 Deposited 2025-03-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
1–431（431 aa）
Chain D
1–431（431 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 5 CA CALCIUM ION × 7 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 A1L80 2-(1-benzofuran-5-yl)-6-[2,6-bis(fluoranyl)-4-[3-(methylamino)propoxy]phenyl]-5-chloranyl-~{N}-[(2~{S})-1,1,1-tris(fluoranyl)propan-2-yl]-[1,2,4]triazolo[1,5-a]pyrimidin-7-amine × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ACP PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；6% PEG 4000, 5% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7
|
Resolution 2.93 Å R-free 0.269 |
| 9YMG Human KIF18A-DARPin fusion protein bound to AMP-PNP and tubulin Deposited 2025-10-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain B
1–444（444 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 EDO 1,2-ETHANEDIOL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.2 M ammonium nitrate, 0.1 M sodium acetate pH 5.6, 20% (w/v) PEG Smear Low (5% PEG 400, 5% PEG 500 MME, 5% PEG 600, 5% PEG 1000), 5% (v/v) ethylene glycol
|
Resolution 2.41 Å R-free 0.238 |
| 9YMG Human KIF18A-DARPin fusion protein bound to AMP-PNP and tubulin Deposited 2025-10-09 | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain E
1–444（444 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；0.2 M ammonium nitrate, 0.1 M sodium acetate pH 5.6, 20% (w/v) PEG Smear Low (5% PEG 400, 5% PEG 500 MME, 5% PEG 600, 5% PEG 1000), 5% (v/v) ethylene glycol
|
Resolution 2.41 Å R-free 0.238 |
| 9YXQ Cryo-EM structure of the Xenopus laevis mitotic centromere-associated kinesin (MCAK) bound to the paclitaxel-stabilized microtubule Deposited 2025-10-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: 14-meric（14） Consistent with protein count |
Chain 2
1–445（445 aa）
Chain 4
1–445（445 aa）
Chain 5
1–445（445 aa）
Chain 7
1–445（445 aa）
Chain B
1–445（445 aa）
Chain b
1–445（445 aa）
|
Not recorded | MG MAGNESIUM ION × 11 GTP GUANOSINE-5'-TRIPHOSPHATE × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 TA1 TAXOL × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.39 Å |