Current Protein Identity：P22141
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1FNT CRYSTAL STRUCTURE OF THE 20S PROTEASOME FROM YEAST IN COMPLEX WITH THE PROTEASOME ACTIVATOR PA26 FROM TRYPANOSOME BRUCEI AT 3.2 ANGSTROMS RESOLUTION Deposited 2000-08-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric（42） Consistent with protein count |
Chain K
1–198（198 aa）
Chain Y
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 14 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；0.1 M NaHEPES, 40% MPD, 0.2 M NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP
|
Resolution 3.20 Å R-free 0.325 |
| 1G0U A GATED CHANNEL INTO THE PROTEASOME CORE PARTICLE Deposited 2000-10-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
hanging drop vapour-diffusion；pH 6.7；297 K；22mM Magnesium acetate, 4% Dimethylsulfoxide, 100mM morpholinoethanesulfonic acid, 11% methylpentanediol, pH 6.7, hanging drop vapour-diffusion, temperature 297K
|
Resolution 2.40 Å R-free 0.303 |
| 1G65 Crystal structure of epoxomicin:20s proteasome reveals a molecular basis for selectivity of alpha,beta-epoxyketone proteasome inhibitors Deposited 2000-11-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；0.1M Mes, pH 6.8
11% MPD
25mM MgAc2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 2.25 Å R-free 0.336 |
| 1JD2 Crystal Structure of the yeast 20S Proteasome:TMC-95A complex: A non-covalent Proteasome Inhibitor Deposited 2001-06-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain Q
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；MPD, MES, DMSO, magnesium Acetate, pH 6.4, VAPOR DIFFUSION, HANGING DROP at 293K
|
Resolution 3.00 Å R-free 0.336 |
| 1RYP CRYSTAL STRUCTURE OF THE 20S PROTEASOME FROM YEAST AT 2.4 ANGSTROMS RESOLUTION Deposited 1997-02-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain K
1–198（198 aa）
Chain Y
1–198（198 aa）
|
Mutation：CHAINS H, V, T1A, CHAIN L, Z, K33R Mutation：CHAINS H, V, T1A, CHAIN L, Z, K33R | MG MAGNESIUM ION × 20 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.9；pH 6.9
|
Resolution 1.90 Å R-free 0.330 |
| 1Z7Q Crystal structure of the 20s proteasome from yeast in complex with the proteasome activator PA26 from Trypanosome brucei at 3.2 angstroms resolution Deposited 2005-03-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric（42） Consistent with protein count |
Chain K
1–198（198 aa）
Chain Y
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.1 M NaHEPES, 40% MPD, 0.2 M NACL, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 3.22 Å R-free 0.308 |
| 2F16 Crystal structure of the yeast 20S proteasome in complex with bortezomib Deposited 2005-11-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；12 % MPD
28mM MgAc2
50mM MES-NaOH, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.264 |
| 2FAK Crystal structure of Salinosporamide A in complex with the yeast 20S proteasome Deposited 2005-12-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | SA1 (3AR,6R,6AS)-6-((S)-((S)-CYCLOHEX-2-ENYL)(HYDROXY)METHYL)-6A-METHYL-4-OXO-HEXAHYDRO-2H-FURO[3,2-C]PYRROLE-6-CARBALDEHYDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；Crystals appeared after one week; Crystallization conditions:
40mg/ml yeast 20S proteasome
0.1M MES; 28mM MgAc2, 12% MPD
6h crystal soak with 10mM SalA, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.254 |
| 2GPL TMC-95 based biphenyl-ether macrocycles: specific proteasome inhibitors Deposited 2006-04-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | BIQ BENZYL [12-(2-AMINO-2-OXOETHYL)-4-NITRO-10,13-DIOXO-15-[(PROPYLAMINO)CARBONYL]-2-OXA-11,14-DIAZATRICYCLO[15 .2.2.1~3,7~]DOCOSA-1(19),3(22),4,6,17,20-HEXAEN-9-YL]CARBAMATE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；20mM MgAc2; 0.1M MES, 12% MPD; crystals appeared within 7 days; crystal size 0.2 x 0.2 x 0.6 mm3, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.81 Å R-free 0.241 |
| 2ZCY yeast 20S proteasome:syringolin A-complex Deposited 2007-11-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | SRG (2S)-2-[[(2S)-1-[[(5S,8S,9E)-2,7-dioxo-5-propan-2-yl-1,6-diazacyclododeca-3,9-dien-8-yl]amino]-3-methyl-1-oxo-butan-2-yl]carbamoylamino]-3-methyl-butanoic acid × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；294 K；0.1M MES, 12% MPD, 30mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 2.90 Å R-free 0.245 |
| 3BDM yeast 20S proteasome:glidobactin A-complex Deposited 2007-11-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | GDT (2E,4E)-N-[(2S,3R)-3-hydroxy-1-[[(3Z,5S,8S,10S)-10-hydroxy-5-methyl-2,7-dioxo-1,6-diazacyclododec-3-en-8-yl]amino]-1-ox obutan-2-yl]dodeca-2,4-dienamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；294 K；100mM Mes, 14% MPD, 30mM MgAc2, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 2.70 Å R-free 0.262 |
| 3DY3 Crystal structure of yeast 20S proteasome in complex with the epimer form of spirolactacystin Deposited 2008-07-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | SLR (3R,4R)-3-hydroxy-2-[(1S)-1-hydroxy-2-methylpropyl]-4-methyl-5-oxo-D-proline × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；0.1 M MES, 10 % MPD, 20 mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.81 Å R-free 0.245 |
| 3DY4 Crystal structure of yeast 20S proteasome in complex with spirolactacystin Deposited 2008-07-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | SLA Omuralide, open form × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；0.1 M MES, 10 % MPD, 20 mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.80 Å R-free 0.256 |
| 3E47 Crystal Structure of the Yeast 20S Proteasome in Complex with Homobelactosin C Deposited 2008-08-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | ESY benzyl N-[(benzyloxy)carbonyl]-L-alanyl-N~6~-[(2R,3S,4S)-3-formyl-2-hydroxy-4-methylhexanoyl]-L-lysinate × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；20% MPD, 0.1M MES, 30mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.00 Å R-free 0.245 |
| 3GPJ Crystal structure of the yeast 20S proteasome in complex with syringolin B Deposited 2009-03-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Fragment：sequence database residues 1-198
Chain X
1–198（198 aa）
Fragment：sequence database residues 1-198
|
Not recorded | SY2 N-{[(1S)-2-methyl-1-{[(5S,8S)-5-(1-methylethyl)-2,7-dioxo-1,6-diazacyclododec-3-en-8-yl]carbamoyl}propyl]carbamoyl}-L-valine × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；12% MPD, 0.1M MES, pH 6.7, 20mM MgAc2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.70 Å R-free 0.264 |
| 3GPT Crystal structure of the yeast 20S proteasome in complex with Salinosporamide derivatives: slow substrate ligand Deposited 2009-03-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Fragment：sequence database residues 1-198
Chain X
1–198（198 aa）
Fragment：sequence database residues 1-198
|
Not recorded | GPT (2R,3S,4R)-2-[(S)-(1S)-cyclohex-2-en-1-yl(hydroxy)methyl]-4-(2-fluoroethyl)-3-hydroxy-3-methyl-5-oxopyrrolidine-2-carbaldehyde × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；12% MPD, 0.1M MES, 20mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.41 Å R-free 0.245 |
| 3GPW Crystal structure of the yeast 20S proteasome in complex with Salinosporamide derivatives: irreversible inhibitor ligand Deposited 2009-03-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Fragment：sequence database residues 1-198
Chain X
1–198（198 aa）
Fragment：sequence database residues 1-198
|
Not recorded | SA1 (3AR,6R,6AS)-6-((S)-((S)-CYCLOHEX-2-ENYL)(HYDROXY)METHYL)-6A-METHYL-4-OXO-HEXAHYDRO-2H-FURO[3,2-C]PYRROLE-6-CARBALDEHYDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.7；12% MPD, 0.1M MES, 20mM MgAc2, pH 6.7, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.50 Å R-free 0.255 |
| 3HYE Crystal structure of 20S proteasome in complex with hydroxylated salinosporamide Deposited 2009-06-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | HYE (2R,3S,4R)-2-[(S)-(1S)-cyclohex-2-en-1-yl(hydroxy)methyl]-3-hydroxy-4-(2-hydroxyethyl)-3-methyl-5-oxopyrrolidine-2-carbaldehyde × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；Crystals grew within one week under 0.1M MES/HCl,pH 6.6, 30mM MgAc2 and 15% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.242 |
| 3JCO Structure of yeast 26S proteasome in M1 state derived from Titan dataset Deposited 2016-01-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain 6
1–198（198 aa）
Chain k
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
50mM Tris pH 7.5, 100mM NaCl, 5mM MgCl2, 2mM ATP；pH 7.5；50mM Tris pH 7.5, 100mM NaCl, 5mM MgCl2, 2mM ATP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å |
| 3JCP Structure of yeast 26S proteasome in M2 state derived from Titan dataset Deposited 2016-01-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain 6
1–198（198 aa）
Chain k
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
50mM Tris pH 7.5, 100mM NaCl, 5mM MgCl2, 2mM ATP；pH 7.5；50mM Tris pH 7.5, 100mM NaCl, 5mM MgCl2, 2mM ATP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å |
| 3MG0 Structure of yeast 20S proteasome with bortezomib Deposited 2010-04-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, pH 7.0, vapor diffusion, hanging drop, temperature 293K
|
Resolution 2.68 Å R-free 0.253 |
| 3MG4 Structure of yeast 20S proteasome with Compound 1 Deposited 2010-04-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 LXT (2S)-2-amino-N-[(1S)-1-({(1S)-1-[(4-methylbenzyl)carbamoyl]-3-phenylpropyl}carbamoyl)-3-phenylpropyl]-4-phenylbutanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, pH 7.0, vapor diffusion, hanging drop, temperature 293K
|
Resolution 3.11 Å R-free 0.250 |
| 3MG6 Structure of yeast 20S open-gate proteasome with Compound 6 Deposited 2010-04-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 LZT N~2~-{[(1S)-6-methoxy-3-oxo-2,3-dihydro-1H-inden-1-yl]acetyl}-N-{(1S)-1-[(4-methylbenzyl)carbamoyl]-3-phenylpropyl}-L-threoninamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, pH 7.0, vapor diffusion, hanging drop, temperature 293K
|
Resolution 2.60 Å R-free 0.249 |
| 3MG7 Structure of yeast 20S open-gate proteasome with Compound 8 Deposited 2010-04-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 L2T 4-(benzyloxy)-N-[(1S,2R)-2-hydroxy-1-({(1S)-1-[(2-methylbenzyl)carbamoyl]-3-phenylpropyl}carbamoyl)propyl]benzamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, pH 7.0, vapor diffusion, hanging drop, temperature 293K
|
Resolution 2.78 Å R-free 0.259 |
| 3MG8 Structure of yeast 20S open-gate proteasome with Compound 16 Deposited 2010-04-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 L3T N-(2,2-dimethylpropyl)-N~2~-[1H-indol-3-yl(oxo)acetyl]-L-asparaginyl-N-(2-methylbenzyl)-3-pyridin-4-yl-L-alaninamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, pH 7.0, vapor diffusion, hanging drop, temperature 293K
|
Resolution 2.59 Å R-free 0.252 |
| 3NZJ Crystal structure of yeast 20S proteasome in complex with ligand 2a Deposited 2010-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.9；293 K；1000 mM MES / HCl, 6.8, 12% MPD, 20 mM MgAc2, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.249 |
| 3NZW Crystal structure of the yeast 20S proteasome in complex with 2b Deposited 2010-07-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；100 mM MES/HCl, 6.8, 20mM MgAc2, 12% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.240 |
| 3NZX Crystal structure of the yeast 20S proteasome in complex with ligand 2c Deposited 2010-07-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；100 mM MES/Hcl, pH 6.8, 20 mM MGAc2, 12 % MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.70 Å R-free 0.248 |
| 3OEU Structure of yeast 20S open-gate proteasome with Compound 24 Deposited 2010-08-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 OEU N-{(2S)-1-[(2-chlorobenzyl)amino]-1-oxo-4-phenylbutan-2-yl}-N~2~-[3-(2-methylphenyl)propanoyl]-L-threoninamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, pH 7.0, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, vapor diffusion, hanging drop, temperature 293K
|
Resolution 2.60 Å R-free 0.249 |
| 3OEV Structure of yeast 20S open-gate proteasome with Compound 25 Deposited 2010-08-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 3OE 4-(benzyloxy)-N-[(2S,3R)-3-hydroxy-1-{[(2S)-1-{[(3-methylthiophen-2-yl)methyl]amino}-1-oxo-4-phenylbutan-2-yl]amino}-1-oxobutan-2-yl]benzamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, pH 7.0, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, vapor diffusion, hanging drop, temperature 293K
|
Resolution 2.85 Å R-free 0.257 |
| 3OKJ Alpha-keto-aldehyde binding mechanism reveals a novel lead structure motif for proteasome inhibition Deposited 2010-08-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | EP9 N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S,3S)-3-hydroxy-1-(4-hydroxyphenyl)-4-oxobutan-2-yl]-L-leucinamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.9；293 K；Crystalls appeared during one week using 100mM MES, 20mM MG2Ac, 12% MPD, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.70 Å R-free 0.259 |
| 3SDI Structure of yeast 20S open-gate proteasome with Compound 20 Deposited 2011-06-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 20 3SD N~4~-(2,2-dimethylpropyl)-N~1~-{(2S)-1-[(4-methylbenzyl)amino]-1-oxo-4-phenylbutan-2-yl}-N~2~-[(5-methyl-1,2-oxazol-3-yl)carbonyl]-L-aspartamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, pH 7.0, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, vapor diffusion, hanging drop, temperature 293K
|
Resolution 2.65 Å R-free 0.253 |
| 3SDK Structure of yeast 20S open-gate proteasome with Compound 34 Deposited 2011-06-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 P3N N-[(2S)-3-(3-tert-butyl-1,2,4-oxadiazol-5-yl)-1-({(2S)-1-[(4-methylbenzyl)amino]-1-oxo-4-phenylbutan-2-yl}amino)-1-oxopropan-2-yl]-5-methyl-1,2-oxazole-3-carboxamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, pH 7.0, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, vapor diffusion, hanging drop, temperature 293K
|
Resolution 2.70 Å R-free 0.259 |
| 3SHJ Proteasome in complex with hydroxyurea derivative HU10 Deposited 2011-06-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 H10 1-hydroxy-1-[(2R)-4-{3-[(3S,5S,7S)-tricyclo[3.3.1.1~3,7~]dec-1-yloxy]phenyl}but-3-yn-2-yl]urea × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20 mM magnesium acetate, 11% PEG, 100 mM MES/NaOH, pH 7.0, crystals grow to their final size within a week, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.266 |
| 3TDD Crystal structure of yeast CP in complex with Belactosin C Deposited 2011-08-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | BFO benzyl N-[(naphthalen-2-ylmethoxy)carbonyl]-L-alanyl-N~5~-[(2R,3S,4S)-3-formyl-2-hydroxy-4-methylhexanoyl]-L-ornithinate × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；100 mM MES, 12% MPD, 20 mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.70 Å R-free 0.236 |
| 3UN4 Yeast 20S proteasome in complex with PR-957 (morpholine) Deposited 2011-11-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；100 mM MES, 12% MPD, 20 mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.40 Å R-free 0.219 |
| 3UN8 Yeast 20S proteasome in complex with PR-957 (epoxide) Deposited 2011-11-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | 049 2-(acetylamino)-4,5-anhydro-1,2-dideoxy-4-methyl-1-phenyl-D-xylitol × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；100 mM MES, 12% MPD, 20 mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.70 Å R-free 0.240 |
| 3WXR Yeast 20S proteasome with a mutation of alpha7 subunit Deposited 2014-08-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain K
1–198（198 aa）
Chain Y
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；14% MPD, 0.1M MES, 50mM Magnesium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.242 |
| 4CR2 Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome Deposited 2014-02-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 33 PDB declaration: 33-meric（33） Consistent with protein count |
Chain 4
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.1
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- HOMEMADE PLUNGER,
|
Resolution 7.70 Å |
| 4CR3 Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome Deposited 2014-02-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 33 PDB declaration: 33-meric（33） Consistent with protein count |
Chain 4
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.1
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- HOMEMADE PLUNGER,
|
Resolution 9.30 Å |
| 4CR4 Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome Deposited 2014-02-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 33 PDB declaration: 33-meric（33） Consistent with protein count |
Chain 4
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.1
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- HOMEMADE PLUNGER,
|
Resolution 8.80 Å |
| 4EU2 Crystal structure of 20s proteasome with novel inhibitor K-7174 Deposited 2012-04-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain K
1–198（198 aa）
Chain Y
1–198（198 aa）
|
Not recorded | WPI 1,4-bis[(4E)-5-(3,4,5-trimethoxyphenyl)pent-4-en-1-yl]-1,4-diazepane × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293 K；4.5% (v/v) 2-methyl1-2,4-pentanediol(MPD), 36mM magnesium acetate, 90mM MES pH7.2, 10%(v/v) DMSO, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.51 Å R-free 0.255 |
| 4FZC 20S yeast proteasome in complex with cepafungin I Deposited 2012-07-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric（32） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；100 mM MES; 20 mM MgAc2; 12% MPD, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.244 |
| 4FZG 20S yeast proteasome in complex with glidobactin Deposited 2012-07-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric（32） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；100 mM MES; 20 mM MgAc2; 12% , pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.00 Å R-free 0.226 |
| 4G4S Structure of Proteasome-Pba1-Pba2 Complex Deposited 2012-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric（32） Consistent with protein count |
Chain K
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 16 LDZ N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S)-4-methyl-1-oxopentan-2-yl]-L-leucinamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；277 K；0.1 M sodium citrate, 18-20% PEG 3000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.49 Å R-free 0.232 |
| 4GK7 yeast 20S proteasome in complex with the Syringolin-Glidobactin chimera Deposited 2012-08-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；0.1M MES, 20mM MgAc2; 12% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.241 |
| 4HNP Crystal structure of yeast 20S proteasome in complex with vinylketone carmaphycin analogue VNK1 Deposited 2012-10-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | ONK N-hexanoyl-L-valyl-N~1~-[(3S,4S)-3-hydroxy-2,6-dimethylhept-1-en-4-yl]-N~5~,N~5~-dimethyl-L-glutamamide × 2 VNK N-hexanoyl-L-valyl-N~1~-[(3S,4S)-3-hydroxy-2,6-dimethylheptan-4-yl]-N~5~,N~5~-dimethyl-L-glutamamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；297 K；30 mM of magnesium acetate, 100 mM of MES (pH 7.2) and 12% of MPD, vapor diffusion, temperature 297K
|
Resolution 2.80 Å R-free 0.239 |
| 4HRC Crystal structure of yeast 20S proteasome in complex with epoxyketone carmaphycin analogue 3 Deposited 2012-10-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | OV2 N-hexanoyl-L-valyl-N~1~-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-N~5~,N~5~-dimethyl-L-glutamamide × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；297 K；30 mM of magnesium acetate, 100 mM of MES (pH 7.2) and 12% of MPD, vapor diffusion, temperature 297K
|
Resolution 2.80 Å R-free 0.243 |
| 4HRD Crystal structure of yeast 20S proteasome in complex with the natural product carmaphycin A Deposited 2012-10-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | OV1 N-[(2S)-1-({(2S)-1-{[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]amino}-4-[(R)-methylsulfinyl]-1-oxobutan-2-yl}amino)-3-methyl-1-oxobutan-2-yl]hexanamide × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；297 K；30 mM of magnesium acetate, 100 mM of MES (pH 7.2) and 12% of MPD, vapor diffusion, temperature 297K
|
Resolution 2.80 Å R-free 0.239 |
| 4INR Yeast 20S proteasome in complex with the vinyl sulfone LU102 Deposited 2013-01-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | 1G1 N3Phe-Leu-Leu-Phe(4-NH2CH2)-methyl vinyl sulfone, bound form × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM magnesium acetate, 0.1 M MES, 12% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.70 Å R-free 0.231 |
| 4INT Yeast 20S proteasome in complex with the vinyl sulfone LU122 Deposited 2013-01-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | 1G5 HMB-Val-Ser-Phe(4-NH2CH2)-methyl vinyl sulfone, bound form × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM magnesium acetate, 0.1 M MES, 12% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.90 Å R-free 0.230 |
| 4INU Yeast 20S proteasome in complex with the vinyl sulfone LU112 Deposited 2013-01-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | 1G6 N3Phe-Phe(4-NH2CH2)-Leu-Phe(4-NH2CH2)-methyl vinyl sulfone, bound form × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.9；293 K；20 mM magnesium acetate, 0.1 M MES, 12% MPD, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.10 Å R-free 0.238 |
| 4J70 Yeast 20S proteasome in complex with the belactosin derivative 3e Deposited 2013-02-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | 1KR benzyl [(2S)-1-({(2R)-1-[(1S,2S)-2-{[(2R,3S,4S)-3-formyl-2-hydroxy-4-methylhexanoyl]amino}cyclopropyl]-4-phenylbutan-2-yl}amino)-1-oxopropan-2-yl]carbamate × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.9；293 K；20 mM magnesium acetate, 0.1 M MES, 12% MPD, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.224 |
| 4JSQ Yeast 20S proteasome in complex with the dimerized linear mimetic of TMC-95A - yCP:4e Deposited 2013-03-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mM MgAc2, 0.1M MES, 12% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.241 |
| 4JSU Yeast 20S proteasome in complex with the dimerized linear mimetic of TMC-95A - yCP:3a Deposited 2013-03-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric（32） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mM MgAc2, 0.1M MES, 12% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.90 Å R-free 0.227 |
| 4JT0 Yeast 20S proteasome in complex with the dimerized linear mimetic of TMC-95A - yCP:4a Deposited 2013-03-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 0L1 hexanedioic acid × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mM MgAc2, 0.1M MES, 12% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.10 Å R-free 0.223 |
| 4LQI Yeast 20S Proteasome in complex with Vibralactone Deposited 2013-07-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | 1Y9 vibralactone, bound form × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20 mM magnesium acetate, 100 mM MES, 12% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.70 Å R-free 0.227 |
| 4LTC Crystal structure of yeast 20S proteasome in complex with enone carmaphycin analogue 6 Deposited 2013-07-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | EC6 N-hexanoyl-L-valyl-N~1~-[(4S,5S,6R)-5-hydroxy-2,6-dimethyloctan-4-yl]-N~5~,N~5~-dimethyl-L-glutamamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；297 K；30 mM of magnesium acetate, 100 mM of MES (pH 7.2) and 12% of MPD, vapor diffusion, temperature 297K
|
Resolution 2.50 Å R-free 0.233 |
| 4NNN yCP in complex with MG132 Deposited 2013-11-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 ALD N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S)-1-hydroxy-4-methylpentan-2-yl]-L-leucinamide × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.249 |
| 4NNW yCP in complex with Z-Leu-Leu-Leu-ketoaldehyde Deposited 2013-11-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 2MK N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2R,3S)-1,2-dihydroxy-5-methylhexan-3-yl]-L-leucinamide × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 100 mM MES, 13% MPD
, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.208 |
| 4NO1 yCP in complex with Z-Leu-Leu-Leu-B(OH)2 Deposited 2013-11-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CIX N-[(benzyloxy)carbonyl]-L-leucyl-N-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-L-leucinamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 100 mM MES, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.214 |
| 4NO6 yCP in complex with Z-Leu-Leu-Leu-vinylsulfone Deposited 2013-11-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 2M1 N-[(benzyloxy)carbonyl]-L-leucyl-N-[(3S)-5-methyl-1-(methylsulfonyl)hexan-3-yl]-L-leucinamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 100 mM MES, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.00 Å R-free 0.207 |
| 4NO8 yCP in complex with Z-Leu-Leu-Leu-ketoamide Deposited 2013-11-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 2LV N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S,3S)-2-hydroxy-5-methyl-1-oxo-1-(phenylamino)hexan-3-yl]-L-leucinamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 100 mM MES, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.70 Å R-free 0.217 |
| 4NO9 yCP in complex with Z-Leu-Leu-Leu-epoxyketone Deposited 2013-11-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 2L0 N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-leucinamide × 3 2LR N-[(benzyloxy)carbonyl]-L-leucyl-N-{(1R,2S)-1-hydroxy-4-methyl-1-[(2R)-2-methyloxiran-2-yl]pentan-2-yl}-L-leucinamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 100 mM MES, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.90 Å R-free 0.203 |
| 4Q1S Yeast 20S proteasome in Complex with Kendomycin Deposited 2014-04-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | 2YD (5R,6R,7S,8R,9R,12S,13E,16S,18S,19R,20aR)-4,7,19-trihydroxy-2,6,8,12,14,16,18-heptamethyl-6,7,8,9,10,11,12,15,16,17,18,19,20,20a-tetradecahydro-1,19:5,9-diepoxybenzo[18]annulen-3(5H)-one × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；20 mM MgAc2, 100 mM MES, 13% MPD, pH 6,8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.224 |
| 4QBY yCP in complex with BOC-ALA-ALA-ALA-CHO Deposited 2014-05-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric（32） Consistent with protein count |
Chain J
1–198（198 aa）
Fragment：beta subunit
Chain X
1–198（198 aa）
Fragment：beta subunit
|
Mutation：wild type Mutation：wild type | MG MAGNESIUM ION × 11 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 100 mM MES, 13% MPD
, pH 6.8 , VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.00 Å R-free 0.207 |
| 4QLQ yCP in complex with tripeptidic epoxyketone inhibitor 8 Deposited 2014-06-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 38N N-(morpholin-4-ylacetyl)-L-alanyl-N-[(2S,4R)-1-cyclohexyl-5-hydroxy-4-methyl-3-oxopentan-2-yl]-O-methyl-L-tyrosinamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20mM MgAc2, 100mM MES, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.215 |
| 4QLS yCP in complex with tripeptidic epoxyketone inhibitor 11 Deposited 2014-06-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 37Y N-(morpholin-4-ylacetyl)-D-alanyl-N-[(2S,4R)-1-cyclohexyl-5-hydroxy-4-methyl-3-oxopentan-2-yl]-O-methyl-L-tyrosinamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20mM MGAC2, 13% MPD, PH 6.8, 100mM MES, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.199 |
| 4QLT yCP in complex with tripeptidic epoxyketone inhibitor 2 (PR924) Deposited 2014-06-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 39V N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-L-tryptophanamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MGAC2, 13% MPD, PH 6.8, 100 mM MES, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.202 |
| 4QLU yCP in complex with tripeptidic epoxyketone inhibitor 9 Deposited 2014-06-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 38X N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-1-cyclohexyl-5-hydroxy-4-methyl-3-oxopentan-2-yl]-L-tryptophanamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20mM MGAC2, 13% MPD, 100mM MES, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.248 |
| 4QLV yCP in complex with tripeptidic epoxyketone inhibitor 17 Deposited 2014-06-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 39Q N-(morpholin-4-ylacetyl)-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-O-methyl-L-tyrosinamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20mM MGAC2, 13% MPD, 100mM MES, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.90 Å R-free 0.209 |
| 4QUX yCP beta5-A49T-mutant Deposited 2014-07-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 3.00 Å R-free 0.197 |
| 4QUY yCP beta5-A49S-mutant Deposited 2014-07-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.80 Å R-free 0.226 |
| 4QV0 yCP beta5-A49T-A50V-double mutant Deposited 2014-07-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 6 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 3.10 Å R-free 0.197 |
| 4QV1 yCP beta5-M45A mutant Deposited 2014-07-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.50 Å R-free 0.216 |
| 4QV3 yCP beta5-M45V mutant Deposited 2014-07-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 3.00 Å R-free 0.211 |
| 4QV4 yCP beta5-M45T mutant Deposited 2014-07-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.70 Å R-free 0.213 |
| 4QV5 yCP beta5-M45I mutant Deposited 2014-07-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.70 Å R-free 0.217 |
| 4QV6 yCP beta5-A49V mutant Deposited 2014-07-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.80 Å R-free 0.231 |
| 4QV7 yCP beta5-A50V mutant Deposited 2014-07-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.60 Å R-free 0.214 |
| 4QV8 yCP beta5-C52F mutant Deposited 2014-07-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.90 Å R-free 0.221 |
| 4QV9 yCP beta5-C63F mutant Deposited 2014-07-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.60 Å R-free 0.218 |
| 4QVL yCP in complex with bortezomib Deposited 2014-07-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.80 Å R-free 0.215 |
| 4QVM yCP beta5-M45A mutant in complex with bortezomib Deposited 2014-07-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.80 Å R-free 0.215 |
| 4QVN yCP beta5-M45V mutant in complex with bortezomib Deposited 2014-07-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.90 Å R-free 0.213 |
| 4QVP yCP beta5-M45T mutant in complex with bortezomib Deposited 2014-07-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.30 Å R-free 0.225 |
| 4QVQ yCP beta5-M45I mutant in complex with bortezomib Deposited 2014-07-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.60 Å R-free 0.219 |
| 4QVV yCP beta5-A49V mutant in complex with bortezomib Deposited 2014-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.80 Å R-free 0.235 |
| 4QVW yCP beta5-A49S-mutant in complex with bortezomib Deposited 2014-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 3.00 Å R-free 0.209 |
| 4QVY yCP beta5-A49T-mutant in complex with bortezomib Deposited 2014-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.51 Å R-free 0.219 |
| 4QW0 yCP beta5-A49T-A50V-double mutant in complex with bortezomib Deposited 2014-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.90 Å R-free 0.211 |
| 4QW1 yCP beta5-A50V mutant in complex with bortezomib Deposited 2014-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.90 Å R-free 0.216 |
| 4QW3 yCP beta5-C63F mutant in complex with bortezomib Deposited 2014-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 5 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.90 Å R-free 0.217 |
| 4QW4 yCP in complex with carfilzomib Deposited 2014-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.80 Å R-free 0.216 |
| 4QW5 yCP beta5-M45A mutant in complex with carfilzomib Deposited 2014-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 5 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 3.00 Å R-free 0.201 |
| 4QW6 yCP beta5-M45V mutant in complex with carfilzomib Deposited 2014-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 6 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.90 Å R-free 0.206 |
| 4QW7 yCP beta5-M45T mutant in complex with carfilzomib Deposited 2014-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.70 Å R-free 0.226 |
| 4QWF yCP beta5-M45I mutant in complex with carfilzomib Deposited 2014-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 3.00 Å R-free 0.203 |
| 4QWG yCP beta5-A49V mutant in complex with carfilzomib Deposited 2014-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 6 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.60 Å R-free 0.243 |
| 4QWI yCP beta5-A49S-mutant in complex with carfilzomib Deposited 2014-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.60 Å R-free 0.214 |
| 4QWJ yCP beta5-A49T-mutant in complex with carfilzomib Deposited 2014-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.90 Å R-free 0.228 |
| 4QWK yCP beta5-A49T-A50V-double mutant in complex with carfilzomib Deposited 2014-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.80 Å R-free 0.209 |
| 4QWL yCP beta5-A50V mutant in complex with carfilzomib Deposited 2014-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.60 Å R-free 0.219 |
| 4QWR yCP beta5-C52F mutant in complex with carfilzomib Deposited 2014-07-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.90 Å R-free 0.209 |
| 4QWS yCP beta5-C63F mutant in complex with carfilzomib Deposited 2014-07-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 MG MAGNESIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 3.00 Å R-free 0.214 |
| 4QWU yCP beta5-C52F mutant in complex with bortezomib Deposited 2014-07-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 3.00 Å R-free 0.218 |
| 4QWX yCP in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 NA SODIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K, pH 6.8
|
Resolution 2.90 Å R-free 0.212 |
| 4QXJ yCP beta5-M45A mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 6 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.80 Å R-free 0.222 |
| 4QZ0 yCP beta5-M45V mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 6 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 3.00 Å R-free 0.201 |
| 4QZ1 yCP beta5-M45T mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 MG MAGNESIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 3.00 Å R-free 0.216 |
| 4QZ2 yCP beta5-M45I mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.70 Å R-free 0.219 |
| 4QZ3 yCP beta5-A49V mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.80 Å R-free 0.207 |
| 4QZ4 yCP beta5-A49S mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 3.00 Å R-free 0.218 |
| 4QZ5 yCP beta5-A49T-mutant in complex with ONX 0914 Deposited 2014-07-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.224 |
| 4QZ6 yCP beta5-A49T-A50V double mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.90 Å R-free 0.204 |
| 4QZ7 yCP beta5-A50V mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.80 Å R-free 0.213 |
| 4QZW yCP beta5-C52F mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 3.00 Å R-free 0.218 |
| 4QZX yCP beta5-C63F mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.60 Å R-free 0.223 |
| 4QZZ yCP in complex with Omuralide Deposited 2014-07-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 SLA Omuralide, open form × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.90 Å R-free 0.208 |
| 4R00 yCP beta5-C52F mutant in complex with Omuralide Deposited 2014-07-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 5 SLA Omuralide, open form × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.80 Å R-free 0.210 |
| 4R02 yCP in complex with BSc4999 (alpha-Keto Phenylamide) Deposited 2014-07-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 11 3E5 N-[(benzyloxy)carbonyl]-L-leucyl-N-{(2S,3S)-1-[(2,4-dimethylphenyl)amino]-2-hydroxy-5-methyl-1-oxohexan-3-yl}-L-leucinamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.50 Å R-free 0.207 |
| 4R17 Ligand-induced aziridine-formation at subunit beta5 of the yeast 20S proteasome Deposited 2014-08-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 11 3K4 (2S,3S)-3-methylaziridine-2-carboxylic acid × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.10 Å R-free 0.198 |
| 4R18 Ligand-induced Lys33-Thr1 crosslinking at subunit beta5 of the yeast 20S proteasome Deposited 2014-08-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 11 ABA ALPHA-AMINOBUTYRIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.40 Å R-free 0.195 |
| 4RUR Yeast 20S proteasome in complex with the alkaloid indolo-phakellin (4) Deposited 2014-11-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 3WE (2E,3aR,14aS)-9-bromo-2-imino-1,2,3,5,6,14a-hexahydro-4H,8H-imidazo[4',5':5,6]pyrrolo[1',2':4,5]pyrazino[1,2-a]indol-8-one × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；297 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K, temperature 297K
|
Resolution 2.50 Å R-free 0.213 |
| 4V7O Proteasome Activator Complex Deposited 2009-12-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain A1
1–198（198 aa）
Chain AO
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 3.00 Å R-free 0.250 |
| 4V7O Proteasome Activator Complex Deposited 2009-12-22 | Assembly 2 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain BK
1–198（198 aa）
Chain BY
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 3.00 Å R-free 0.250 |
| 4X6Z Yeast 20S proteasome in complex with PR-VI modulator Deposited 2014-12-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain K
1–198（198 aa）
Chain Y
1–198（198 aa）
|
Not recorded | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 9 GOL GLYCEROL × 38 MG MAGNESIUM ION × 56 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；30mM magnesium acetate, 12% MPD, 0.1M MES pH 7.2
|
Resolution 2.70 Å R-free 0.222 |
| 4Y69 Yeast 20S proteasome in complex with Ac-PAD-ep Deposited 2015-02-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR
DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.90 Å R-free 0.217 |
| 4Y6A Yeast 20S proteasome beta2-H114D mutant in complex with Ac-PAD-ep Deposited 2015-02-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR
DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.60 Å R-free 0.232 |
| 4Y6V Yeast 20S proteasome in complex with Ac-PAE-ep Deposited 2015-02-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.209 |
| 4Y6Z Yeast 20S proteasome in complex with Ac-PAL-ep Deposited 2015-02-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.216 |
| 4Y70 Yeast 20S proteasome in complex with Ac-LAV-ep Deposited 2015-02-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric（32） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.40 Å R-free 0.226 |
| 4Y74 Yeast 20S proteasome in complex with Ac-LAL-ep Deposited 2015-02-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.214 |
| 4Y75 Yeast 20S proteasome in complex with Ac-PAF-ep Deposited 2015-02-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric（32） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.215 |
| 4Y77 Yeast 20S proteasome in complex with Ac-LAF-ep Deposited 2015-02-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.50 Å R-free 0.215 |
| 4Y78 Yeast 20S proteasome in complex with Ac-LAD-ep Deposited 2015-02-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.219 |
| 4Y7W Yeast 20S proteasome in complex with Ac-LAE-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.50 Å R-free 0.217 |
| 4Y7X Yeast 20S proteasome in complex with Ac-PAA-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.225 |
| 4Y7Y Yeast 20S proteasome in complex with Ac-LAA-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric（32） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.40 Å R-free 0.235 |
| 4Y80 Yeast 20S proteasome in complex with Ac-LAI-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.50 Å R-free 0.219 |
| 4Y81 Yeast 20S proteasome in complex with Ac-PAY-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric（32） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.234 |
| 4Y82 Yeast 20S proteasome in complex with Ac-LAY-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.207 |
| 4Y84 Yeast 20S proteasome in complex with N3-A(4,4-F2P)nLL-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.203 |
| 4Y8G Yeast 20S proteasome in complex with N3-APnLL-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.208 |
| 4Y8H Yeast 20S proteasome in complex with N3-APAL-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.50 Å R-free 0.214 |
| 4Y8I Yeast 20S proteasome in complex with Ac-PLL-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.220 |
| 4Y8J Yeast 20S proteasome in complex with Ac-LLL-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.224 |
| 4Y8K Yeast 20S proteasome in complex with H-APLL-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric（32） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.236 |
| 4Y8L Yeast 20S proteasome in complex with Ac-APLL-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric（32） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.40 Å R-free 0.224 |
| 4Y8M Yeast 20S proteasome beta7-delta7_Cter mutant Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.218 |
| 4Y8N Yeast 20S proteasome beta7-delta7_Cter mutant in complex with Ac-PAE-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.215 |
| 4Y8O Yeast 20S proteasome beta7-delta7_Cter mutant in complex with Ac-PAF-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric（32） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.231 |
| 4Y8P Yeast 20S proteasome beta7-delta7_Cter mutant in complex with Ac-PAL-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.220 |
| 4Y8Q Yeast 20S proteasome beta7-delta7_Cter mutant in complex with Ac-PAY-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric（32） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.228 |
| 4Y8R Yeast 20S proteasome beta2-H116D mutant Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.222 |
| 4Y8S Yeast 20S proteasome beta2-H116D mutant in complex with Ac-LAE-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.221 |
| 4Y8T Yeast 20S proteasome beta2-H116D mutant in complex with Ac-PAE-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.209 |
| 4Y8U Yeast 20S proteasome beta2-H116D mutant in complex with Ac-PAD-ep Deposited 2015-02-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 6 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.214 |
| 4Y9Y Yeast 20S proteasome beta2-H116E mutant Deposited 2015-02-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.203 |
| 4Y9Z Yeast 20S proteasome beta2-H116E mutant in complex with Ac-LAE-ep Deposited 2015-02-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.218 |
| 4YA0 Yeast 20S proteasome beta2-H116E mutant in complex with Ac-PAE-ep Deposited 2015-02-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.216 |
| 4YA1 Yeast 20S proteasome beta2-H116N mutant Deposited 2015-02-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | CL CHLORIDE ION × 4 MG MAGNESIUM ION × 9 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.218 |
| 4YA2 Yeast 20S proteasome beta2-H116N mutant in complex with Ac-LAE-ep Deposited 2015-02-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.205 |
| 4YA3 Yeast 20S proteasome beta2-H116N mutant in complex with Ac-PAE-ep Deposited 2015-02-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.220 |
| 4YA4 Yeast 20S proteasome beta2-H114D mutant Deposited 2015-02-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.232 |
| 4YA5 Yeast 20S proteasome beta2-H114D mutant in complex with Ac-PAE-ep Deposited 2015-02-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.50 Å R-free 0.242 |
| 4YA7 Yeast 20S proteasome beta2-H114D mutant in complex with Ac-LAE-ep Deposited 2015-02-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.237 |
| 4YA9 Yeast 20S proteasome beta2-H114D mutant in complex with Ac-LAD-ep Deposited 2015-02-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 CL CHLORIDE ION × 1 K POTASSIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.241 |
| 4Z1L Yeast 20S proteasome in complex with belactosin C derivative 3 Deposited 2015-03-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 4KF (2S,3S)-2-{(1R)-2-[(3,5-dimethoxybenzyl)amino]-1-hydroxy-2-oxoethyl}-3-methylpentanoic acid × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 3.00 Å R-free 0.229 |
| 5A5B Structure of the 26S proteasome-Ubp6 complex Deposited 2015-06-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric（35） Consistent with protein count |
Chain 4
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- HOMEMADE PLUNGER,
|
Resolution 9.50 Å |
| 5AHJ Yeast 20S proteasome in complex with Macyranone A Deposited 2015-02-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 7IM N-[(2S)-3-{[(1S)-1-carboxy-2-phenylethyl]amino}-2-methyl-3-oxopropanoyl]-L-threonyl-N-[(3S,4S)-1,3-dihydroxy-6-methylheptan-4-yl]-L-allothreoninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.8；100 MM MES, PH 7.0 12% MPD 20 MM MGAC2
|
Resolution 2.80 Å R-free 0.219 |
| 5BOU Yeast 20S proteasome in complex with a beta1 / beta2 specific non-peptidic sulfonamide Ligand Deposited 2015-05-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 4UC N-[4-(acetylsulfamoyl)phenyl]-2-(4-ethoxyphenyl)quinoline-4-carboxamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.214 |
| 5BXL Yeast 20S proteasome beta2-G170A mutant Deposited 2015-06-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.214 |
| 5BXN Yeast 20S proteasome beta2-G170A mutant in complex with Bortezomib Deposited 2015-06-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.218 |
| 5CGF Yeast 20S proteasome beta5-G48C mutant Deposited 2015-07-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.201 |
| 5CGG Yeast 20S proteasome beta5-G48C mutant in complex with alpha-chloroacetamide 1 Deposited 2015-07-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.231 |
| 5CGH Yeast 20S proteasome beta5-G48C mutant in complex with alpha-chloroacetamide 5 Deposited 2015-07-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 54L pent-4-ynoic acid × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.50 Å R-free 0.227 |
| 5CGI Yeast 20S proteasome beta5-G48C mutant in complex with ONX 0914 Deposited 2015-07-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 NA SODIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.206 |
| 5CZ4 Yeast 20S proteasome at 2.3 A resolution Deposited 2015-07-31 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.30 Å R-free 0.228 |
| 5CZ5 Yeast 20S proteasome beta1-T1A mutant in complex with Carfilzomib Deposited 2015-07-31 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.222 |
| 5CZ6 Yeast 20S proteasome beta5-T1A mutant in complex with Syringolin A, propeptide expressed in trans Deposited 2015-07-31 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 SRG (2S)-2-[[(2S)-1-[[(5S,8S,9E)-2,7-dioxo-5-propan-2-yl-1,6-diazacyclododeca-3,9-dien-8-yl]amino]-3-methyl-1-oxo-butan-2-yl]carbamoylamino]-3-methyl-butanoic acid × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.226 |
| 5CZ7 Yeast 20S proteasome beta5-T1A beta5-K81R double mutant in complex with Bortezomib, propeptide expressed in cis Deposited 2015-07-31 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 11 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.50 Å R-free 0.215 |
| 5CZ8 Yeast 20S proteasome beta5-L(-49)S-K33A mutant in complex with Carfilzomib Deposited 2015-07-31 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.215 |
| 5CZ9 Yeast 20S proteasome beta5-D17N mutant in complex with Carfilzomib; Propeptide expressed in trans Deposited 2015-07-31 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.223 |
| 5CZA Yeast 20S proteasome beta5-D166N mutant Deposited 2015-07-31 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.50 Å R-free 0.224 |
| 5D0S Yeast 20S proteasome beta5-D166N mutant in complex with Carfilzomib Deposited 2015-08-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.50 Å R-free 0.237 |
| 5D0T Yeast 20S proteasome beta5-D166N mutant in complex with MG132 Deposited 2015-08-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 11 CL CHLORIDE ION × 4 ALD N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S)-1-hydroxy-4-methylpentan-2-yl]-L-leucinamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.218 |
| 5D0V Yeast 20S proteasome beta5-T1C mutant in complex with Carfilzomib Deposited 2015-08-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.220 |
| 5D0W Yeast 20S proteasome beta5-T1S mutant Deposited 2015-08-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.203 |
| 5D0X Yeast 20S proteasome beta5-T1S mutant in complex with Bortezomib Deposited 2015-08-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.224 |
| 5D0Z Yeast 20S proteasome beta5-T1S mutant in complex with Carfilzomib Deposited 2015-08-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.207 |
| 5DKI Yeast 20S proteasome in complex with alkyne-PI Deposited 2015-09-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 5 5BZ [(1~{R})-1-[[(2~{S})-2-(hex-5-ynoylamino)-3-phenyl-propanoyl]amino]-3-methyl-butyl]boronic acid × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；30 mM MgAc2, 100 mM MES, 10% MPD
|
Resolution 2.80 Å R-free 0.219 |
| 5DKJ Yeast 20S proteasome in complex with octreotide-PI Deposited 2015-09-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 5BY {2-[2-(2-{4-[(1E)-4-{[(2S)-1-{[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]amino}-1-oxo-3-phenylpropan-2-yl]amino}-4-oxobut-1-en-1-yl]-1H-1,2,3-triazol-1-yl}ethoxy)ethoxy]ethoxy}acetic acid × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；30 mM MgAC2, 10% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.210 |
| 5FG7 Yeast 20S proteasome beta2-T1A mutant Deposited 2015-12-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.231 |
| 5FG9 Yeast 20S proteasome beta2-T(-2)V mutant Deposited 2015-12-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.230 |
| 5FGA Yeast 20S proteasome beta5-K33A mutant (propeptide expressed in trans) Deposited 2015-12-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.214 |
| 5FGD Yeast 20S proteasome beta5-H(-2)L-T1A double mutant in complex with Carfilzomib Deposited 2015-12-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 5 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.208 |
| 5FGE Yeast 20S proteasome beta5-H(-2)T-T1A double mutant in complex with Carfilzomib Deposited 2015-12-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.214 |
| 5FGF Yeast 20S proteasome beta5-H(-2)A-T1A-K81R triple mutant in complex with Carfilzomib Deposited 2015-12-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.214 |
| 5FGG Yeast 20S proteasome beta5-L(-49S)_D17N double mutant in complex with Carfilzomib Deposited 2015-12-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.208 |
| 5FGH Yeast 20S proteasome beta5-K33A mutant (propeptide expressed in trans) in complex with MG132 Deposited 2015-12-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 ALD N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S)-1-hydroxy-4-methylpentan-2-yl]-L-leucinamide × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.206 |
| 5FGI Yeast 20S proteasome beta1-T1A beta2-T1A double mutant in complex with Carfilzomib Deposited 2015-12-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 6 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.210 |
| 5FHS Yeast 20S proteasome beta5-K33A mutant (propeptide expressed in trans) in complex with Carfilzomib Deposited 2015-12-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.212 |
| 5JHR Yeast 20S proteasome in complex with the peptidic epoxyketone inhibitor 27 Deposited 2016-04-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 3 6KF (2S)-2-azido-N-[(2S)-3-(biphenyl-4-yl)-1-{[(2S)-1-{[(2S,3S,4R)-3,5-dihydroxy-4-methylpentan-2-yl]amino}-1-oxo-3-phenylpropan-2-yl]amino}-1-oxopropan-2-yl]-3-phenylpropanamide (non-preferred name) × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.206 |
| 5JHS Yeast 20S proteasome in complex with the peptidic epoxyketone inhibitor 15 Deposited 2016-04-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 6KG Nalpha-[(2S)-2-{[(2S)-2-azido-3-phenylpropanoyl]amino}-4-cyclohexylbutanoyl]-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 3.00 Å R-free 0.203 |
| 5L52 Yeast 20S proteasome in complex with epoxyketone inhibitor 14 Deposited 2016-05-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 6N5 ~{N}-[(2~{S})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；292 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.210 |
| 5L54 Yeast 20S proteasome in complex with epoxyketone inhibitor 16 Deposited 2016-05-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 79P (2~{S})-3-(1~{H}-indol-3-yl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.206 |
| 5L55 Yeast 20S proteasome in complex with epoxyketone inhibitor 18 Deposited 2016-05-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 11 CL CHLORIDE ION × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 6NV ~{N}-[(2~{R})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.225 |
| 5L5A Yeast 20S proteasome with human beta5i (1-138; R57T) Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.40 Å R-free 0.219 |
| 5L5B Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.211 |
| 5L5D Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with ONX 0914 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.221 |
| 5L5E Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with carfilzomib Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.227 |
| 5L5F Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with bortezomib Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.50 Å R-free 0.228 |
| 5L5H Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with PR-924 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 39V N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-L-tryptophanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.216 |
| 5L5I Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 9 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 38X N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-1-cyclohexyl-5-hydroxy-4-methyl-3-oxopentan-2-yl]-L-tryptophanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.210 |
| 5L5J Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 14 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 6N5 ~{N}-[(2~{S})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.200 |
| 5L5O Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 16 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 79P (2~{S})-3-(1~{H}-indol-3-yl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.214 |
| 5L5P Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 17 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 79L (2~{S})-3-(4-methoxyphenyl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.207 |
| 5L5Q Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone 18 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 6NV ~{N}-[(2~{R})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.210 |
| 5L5R Yeast 20S proteasome with human beta5i (1-138;V31M) and human beta6 (97-111; 118-133) Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.221 |
| 5L5S Yeast 20S proteasome with human beta5i (1-138; V31M) and human beta6 (97-111; 118-133) in complex with PR-924 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 39V N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-L-tryptophanamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.211 |
| 5L5T Yeast 20S proteasome with human beta5i (1-138; V31M) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 16 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 79P (2~{S})-3-(1~{H}-indol-3-yl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.195 |
| 5L5U Yeast 20S proteasome with human beta5i (1-138; V31M) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 17 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 11 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.211 |
| 5L5V 'Yeast 20S proteasome with human beta5i (1-138; V31M) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 18 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 6NV ~{N}-[(2~{R})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.208 |
| 5L5W Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | CL CHLORIDE ION × 4 MG MAGNESIUM ION × 12 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.212 |
| 5L5X Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with ONX 0914 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.215 |
| 5L5Y Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with carfilzomib Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.207 |
| 5L5Z Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with bortezomib Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.212 |
| 5L60 Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with PR-924 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 11 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 39V N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-L-tryptophanamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.212 |
| 5L61 Yeast 20S proteasome with human beta5c (1-138) and human beta6 (99-132) in complex with epoxyketone inhibitor 14 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 11 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 6N5 ~{N}-[(2~{S})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.205 |
| 5L62 Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 16 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 12 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 79P (2~{S})-3-(1~{H}-indol-3-yl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.204 |
| 5L63 Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 17 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 11 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.212 |
| 5L64 Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 18 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 6NV ~{N}-[(2~{R})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.223 |
| 5L65 Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with carfilzomib Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.201 |
| 5L66 Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with bortezomib Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.210 |
| 5L67 Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with PR-924 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 39V N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-L-tryptophanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.213 |
| 5L68 Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 14 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 6N5 ~{N}-[(2~{S})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.223 |
| 5L69 Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 16 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 3 79P (2~{S})-3-(1~{H}-indol-3-yl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.217 |
| 5L6A Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 17 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 79L (2~{S})-3-(4-methoxyphenyl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.213 |
| 5L6B Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with ONX 0914 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.221 |
| 5L6C Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 18 Deposited 2016-05-28 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 6NV ~{N}-[(2~{R})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.217 |
| 5LAI Ligand-induced aziridine-formation at the yeast proteasomal subunit beta5 by sulfonate esters Deposited 2016-06-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 11 3K4 (2S,3S)-3-methylaziridine-2-carboxylic acid × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 13% MPD
|
Resolution 2.50 Å R-free 0.219 |
| 5LAJ Ligand-induced Lys33-Thr1 crosslinking at the yeast proteasomal subunit beta5 by sulfonate esters Deposited 2016-06-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 11 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MGAC2, 13% MPD
|
Resolution 2.90 Å R-free 0.201 |
| 5LTT Yeast 20S proteasome with human beta5i (1-138; R57T)in complex with PR-924 Deposited 2016-09-07 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 39V N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-L-tryptophanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.247 |
| 5M2B Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with thiazole based inhibitor Ro19 Deposited 2016-10-12 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 7DX (2~{S})-2-cyclohexyl-4-oxidanylidene-4-[[7-(4-phenyl-1,3-thiazol-2-yl)quinolin-2-yl]amino]butanoic acid × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.218 |
| 5MP9 26S proteasome in presence of ATP (s1) Deposited 2016-12-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain 4
1–198（198 aa）
Chain k
1–198（198 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.10 Å |
| 5MPA 26S proteasome in presence of ATP (s2) Deposited 2016-12-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain 4
1–198（198 aa）
Chain k
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.50 Å |
| 5MPB 26S proteasome in presence of AMP-PNP (s3) Deposited 2016-12-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain 4
1–198（198 aa）
Chain k
1–198（198 aa）
|
Not recorded | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 7.80 Å |
| 5MPC 26S proteasome in presence of BeFx (s4) Deposited 2016-12-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric（48） Consistent with protein count |
Chain 4
1–198（198 aa）
Chain k
1–198（198 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 7.70 Å |
| 5NIF Yeast 20S proteasome in complex with Blm-pep activator Deposited 2017-03-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain K
1–198（198 aa）
Chain Y
1–198（198 aa）
|
Not recorded | CL CHLORIDE ION × 12 MG MAGNESIUM ION × 8 EDO 1,2-ETHANEDIOL × 16 GOL GLYCEROL × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1M MES pH 6.5, 35mM magnesium acetate, 13% MPD
|
Resolution 3.00 Å R-free 0.227 |
| 5WVI The resting state of yeast proteasome Deposited 2016-12-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain 4
1–198（198 aa）
Chain g
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.30 Å |
| 5WVK Yeast proteasome-ADP-AlFx Deposited 2016-12-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain 4
1–198（198 aa）
Chain g
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6EF3 Yeast 26S proteasome bound to ubiquitinated substrate (4D motor state) Deposited 2018-08-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric（24） Consistent with protein count |
Chain 4
1–198（198 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；specimens were manually blotted with Whatman #1 filter paper
|
Resolution 4.17 Å |
| 6FVT 26S proteasome, s1 state Deposited 2018-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain 4
1–195（195 aa）
Chain k
1–195（195 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.10 Å |
| 6FVU 26S proteasome, s2 state Deposited 2018-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain 4
1–195（195 aa）
Chain k
1–195（195 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.50 Å |
| 6FVV 26S proteasome, s3 state Deposited 2018-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain 4
1–195（195 aa）
Chain k
1–195（195 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 2 MG MAGNESIUM ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 5.40 Å |
| 6FVW 26S proteasome, s4 state Deposited 2018-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain 4
1–195（195 aa）
Chain k
1–195（195 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.50 Å |
| 6FVX 26S proteasome, s5 state Deposited 2018-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain 4
1–195（195 aa）
Chain k
1–195（195 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 4.90 Å |
| 6FVY 26S proteasome, s6 state Deposited 2018-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain 4
1–195（195 aa）
Chain k
1–195（195 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 6.10 Å |
| 6G7F Yeast 20S proteasome in complex with Cystargolide B Deposited 2018-04-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 EPW Cystargolide B- bound form × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8
|
Resolution 2.70 Å R-free 0.214 |
| 6G8M Yeast 20S proteasome in complex with Cystargolide B Derivative 1 Deposited 2018-04-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 EQE (2~{S},3~{R})-4-[[(2~{S})-3-methyl-1-[[(2~{S})-3-methyl-1-oxidanylidene-1-phenylmethoxy-butan-2-yl]amino]-1-oxidanylidene-butan-2-yl]amino]-3-oxidanyl-4-oxidanylidene-2-propan-2-yl-butanoic acid × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8
|
Resolution 2.70 Å R-free 0.214 |
| 6G8N Yeast 20S proteasome in complex with Cystargolide B Derivative 2 Deposited 2018-04-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 EQB (2~{S},3~{S})-3-methyl-2-[(1~{R})-2-[[(2~{S})-3-methyl-1-[[(2~{S})-3-methyl-1-oxidanylidene-1-phenylmethoxy-butan-2-yl] amino]-1-oxidanylidene-butan-2-yl]amino]-1-oxidanyl-2-oxidanylidene-ethyl]pentanoic acid × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8
|
Resolution 3.00 Å R-free 0.238 |
| 6GOP Yeast 20S Proteasome in complex with Homosalinosporamide A Deposited 2018-06-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 F6K Homosalinosporamide A - bound form × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.233 |
| 6H39 Yeast 20S proteasome in complex with the peptidic non-covalent binding inhibitor RTS-V5 Deposited 2018-07-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 FGY N-(2,2-dimethylpropyl)-N~2~-[4-(hydroxycarbamoyl)benzene-1-carbonyl]-L-asparaginyl-N-benzyl-L-alaninamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.50 Å R-free 0.217 |
| 6HTB Yeast 20S proteasome with human beta2c (S171G) Deposited 2018-10-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.220 |
| 6HTC Yeast 20S proteasome with human beta2c (S171G) in complex with ONX 0914 Deposited 2018-10-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 GQK (2~{S})-3-(4-methoxyphenyl)-~{N}-[(2~{S},3~{R})-4-methyl-3,4-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.218 |
| 6HTD Yeast 20S proteasome with human beta2c (S171G) in complex with 4 Deposited 2018-10-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQH (2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 3.00 Å R-free 0.201 |
| 6HTP Yeast 20S proteasome with human beta2c (S171G) in complex with 7 Deposited 2018-10-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQQ ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]pyrazine-2-carboxamide × 4 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 3.00 Å R-free 0.206 |
| 6HTR Yeast 20S proteasome with human beta2c (S171G) in complex with 13 Deposited 2018-10-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 GQT (2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.225 |
| 6HUB Yeast 20S proteasome with human beta2c (S171G) in complex with 16 Deposited 2018-10-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GRW (2~{S})-~{N}-[(2~{S},3~{R})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-butan-2-yl]-2-[[(2~{R})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.212 |
| 6HUC Yeast 20S proteasome with human beta2c (S171G) in complex with 18 Deposited 2018-10-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GRT (2~{S})-~{N}-[2-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-2-oxidanylidene-ethyl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 3.00 Å R-free 0.218 |
| 6HUQ Yeast 20S proteasome with human beta2c (S171G) in complex with 20 Deposited 2018-10-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GT5 ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-methoxy-1-oxidanylidene-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-2-methyl-1,3-thiazole-5-carboxamide × 4 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 3.00 Å R-free 0.207 |
| 6HUU Yeast 20S proteasome with human beta2c (S171G) in complex with 29 Deposited 2018-10-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GTW ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-cyclohexyl-1-oxidanylidene-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-2-methyl-1,3-thiazole-5-carboxamide × 4 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.221 |
| 6HUV Yeast 20S proteasome with human beta2c (S171G) in complex with 39 Deposited 2018-10-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GT8 (2~{S})-~{N}-[(3~{S},4~{R})-1-cyclohexyl-5-methyl-4,5-bis(oxidanyl)hexan-3-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 6 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 3.10 Å R-free 0.213 |
| 6HV3 Yeast 20S proteasome with human beta2i (1-53) Deposited 2018-10-10 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.209 |
| 6HV4 Yeast 20S proteasome with human beta2i (1-53) in complex with ONX 0914 Deposited 2018-10-10 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQK (2~{S})-3-(4-methoxyphenyl)-~{N}-[(2~{S},3~{R})-4-methyl-3,4-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 3.00 Å R-free 0.214 |
| 6HV5 Yeast 20S proteasome with human beta2i (1-53) in complex with 4 Deposited 2018-10-10 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQH (2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 3.00 Å R-free 0.204 |
| 6HV7 Yeast 20S proteasome with human beta2i (1-53) in complex with 7 Deposited 2018-10-10 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQQ ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]pyrazine-2-carboxamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 3.40 Å R-free 0.229 |
| 6HVA Yeast 20S proteasome with human beta2i (1-53) in complex with 13 Deposited 2018-10-10 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQT (2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.208 |
| 6HVR Yeast 20S proteasome with human beta2i (1-53) in complex with 16 Deposited 2018-10-11 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GRW (2~{S})-~{N}-[(2~{S},3~{R})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-butan-2-yl]-2-[[(2~{R})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.219 |
| 6HVS Yeast 20S proteasome with human beta2i (1-53) in complex with 18 Deposited 2018-10-11 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GRT (2~{S})-~{N}-[2-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-2-oxidanylidene-ethyl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 3.10 Å R-free 0.205 |
| 6HVT Yeast 20S proteasome with human beta2i (1-53) in complex with 20 Deposited 2018-10-11 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GT5 ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-methoxy-1-oxidanylidene-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-2-methyl-1,3-thiazole-5-carboxamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.206 |
| 6HVU Yeast 20S proteasome with human beta2i (1-53) in complex with 29 Deposited 2018-10-11 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GTW ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-cyclohexyl-1-oxidanylidene-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-2-methyl-1,3-thiazole-5-carboxamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.220 |
| 6HVV Yeast 20S proteasome with human beta2i (1-53) in complex with 39 Deposited 2018-10-11 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GT8 (2~{S})-~{N}-[(3~{S},4~{R})-1-cyclohexyl-5-methyl-4,5-bis(oxidanyl)hexan-3-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.219 |
| 6HVW Yeast 20S proteasome with human beta2i (1-53) in complex with 43 Deposited 2018-10-11 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GVW (2~{S})-~{N}-[(2~{S},3~{R})-1-[(4~{a}~{S},8~{a}~{S})-1,2,3,4,4~{a},5,6,7,8,8~{a}-decahydronaphthalen-2-yl]-4-methyl-3,4-bis(oxidanyl)pentan-2-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 3.00 Å R-free 0.202 |
| 6HVX Yeast 20S proteasome in complex with 4 Deposited 2018-10-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 GQH (2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.206 |
| 6HVY Yeast 20S proteasome in complex with 5 (7- and 6-membered ring) Deposited 2018-10-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GVZ (2~{S})-~{N}-[(2~{S},3~{R})-1-[(1~{S},4~{a}~{S},8~{a}~{R})-1,2,3,4,4~{a},5,6,7,8,8~{a}-decahydronaphthalen-1-yl]-4-methyl-3,4-bis(oxidanyl)pentan-2-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 2 GW2 (2~{S})-~{N}-[(2~{S},3~{S},4~{R})-1-[(1~{S},4~{a}~{S},8~{a}~{R})-1,2,3,4,4~{a},5,6,7,8,8~{a}-decahydronaphthalen-1-yl]-4-methyl-3,5-bis(oxidanyl)pentan-2-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.218 |
| 6HW0 Yeast 20S proteasome in complex with 7 Deposited 2018-10-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQQ ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]pyrazine-2-carboxamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.206 |
| 6HW3 Yeast 20S proteasome in complex with 13 Deposited 2018-10-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQT (2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.215 |
| 6HW4 Yeast 20S proteasome in complex with 16 Deposited 2018-10-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GRW (2~{S})-~{N}-[(2~{S},3~{R})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-butan-2-yl]-2-[[(2~{R})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.210 |
| 6HW5 Yeast 20S proteasome in complex with 18 Deposited 2018-10-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GRT (2~{S})-~{N}-[2-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-2-oxidanylidene-ethyl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.211 |
| 6HW6 Yeast 20S proteasome in complex with 20 Deposited 2018-10-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 GT5 ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-methoxy-1-oxidanylidene-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-2-methyl-1,3-thiazole-5-carboxamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.206 |
| 6HW7 Yeast 20S proteasome in complex with 29 Deposited 2018-10-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 GTW ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-cyclohexyl-1-oxidanylidene-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-2-methyl-1,3-thiazole-5-carboxamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.225 |
| 6HW8 Yeast 20S proteasome in complex with 39 Deposited 2018-10-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 GT8 (2~{S})-~{N}-[(3~{S},4~{R})-1-cyclohexyl-5-methyl-4,5-bis(oxidanyl)hexan-3-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.206 |
| 6HW9 Yeast 20S proteasome in complex with 41b Deposited 2018-10-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 GWK (2~{S})-3-(4-methoxyphenyl)-~{N}-[(2~{S},3~{R})-4-methyl-1-(4-methylcyclohexyl)-3,4-bis(oxidanyl)pentan-2-yl]-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.205 |
| 6HWA Yeast 20S proteasome in complex with 43 Deposited 2018-10-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 GVW (2~{S})-~{N}-[(2~{S},3~{R})-1-[(4~{a}~{S},8~{a}~{S})-1,2,3,4,4~{a},5,6,7,8,8~{a}-decahydronaphthalen-2-yl]-4-methyl-3,4-bis(oxidanyl)pentan-2-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.216 |
| 6HWB Yeast 20S proteasome in complex with 44b Deposited 2018-10-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 GWT (2~{S})-~{N}-[(2~{S},3~{R})-1-(4-cyclohexylcyclohexyl)-4-methyl-3,4-bis(oxidanyl)pentan-2-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ium-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.221 |
| 6HWC Yeast 20S proteasome beta2-G45A mutant Deposited 2018-10-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.224 |
| 6HWD Yeast 20S proteasome beta2-G45A mutant in complex with bortezomib Deposited 2018-10-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.211 |
| 6HWE Yeast 20S proteasome beta2-G45A mutant in complex with carfilzomib Deposited 2018-10-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GWZ (2~{S})-~{N}-[(2~{S})-1-[[(3~{R},4~{S})-2,6-dimethyl-2,3-bis(oxidanyl)heptan-4-yl]amino]-1-oxidanylidene-3-phenyl-propan-2-yl]-4-methyl-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)-4-phenyl-butanoyl]amino]pentanamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.30 Å R-free 0.231 |
| 6HWF Yeast 20S proteasome beta2-G45A mutant in complex with ONX 0914 Deposited 2018-10-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQK (2~{S})-3-(4-methoxyphenyl)-~{N}-[(2~{S},3~{R})-4-methyl-3,4-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.50 Å R-free 0.216 |
| 6J2C Yeast proteasome in translocation competent state (C3-a) Deposited 2019-01-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain 4
1–198（198 aa）
Chain g
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.00 Å |
| 6J2N yeast proteasome in substrate-processing state (C3-b) Deposited 2019-01-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain 4
1–198（198 aa）
Chain g
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.50 Å |
| 6J2Q Yeast proteasome in Ub-accepted state (C1-b) Deposited 2019-01-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain 4
1–198（198 aa）
Chain g
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6J2X Yeast proteasome in resting state (C1-a) Deposited 2019-01-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain 4
1–198（198 aa）
Chain g
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6J30 yeast proteasome in Ub-engaged state (C2) Deposited 2019-01-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain 4
1–198（198 aa）
Chain g
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 6ZOU Yeast 20S proteasome in complex with glidobactin-like natural product HB333 Deposited 2020-07-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 QOH 11-methyl-~{N}-[(2~{S},3~{R})-1-[[(5~{S},8~{S},10~{S})-5-methyl-10-oxidanyl-2,7-bis(oxidanylidene)-1,6-diazacyclododec-8-yl]amino]-3-oxidanyl-1-oxidanylidene-butan-2-yl]dodecanamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.212 |
| 6ZP6 Yeast 20S proteasome in complex with glidobactin-like natural product HB334 Deposited 2020-07-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 QOB ~{N}-[(2~{S},3~{R})-1-[[(5~{S},8~{S},10~{S})-5-methyl-10-oxidanyl-2,7-bis(oxidanylidene)-1,6-diazacyclododec-8-yl]amino]-3-oxidanyl-1-oxidanylidene-butan-2-yl]-5-phenyl-pentanamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.202 |
| 6ZP8 Yeast 20S proteasome in complex with glidobactin-like natural product HB335 Deposited 2020-07-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 QOE (2~{S},3~{R})-~{N}-[(5~{S},8~{S},10~{S})-5-methyl-10-oxidanyl-2,7-bis(oxidanylidene)-1,6-diazacyclododec-8-yl]-3-oxidanyl-2-(3-phenylpropanoylamino)butanamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 3.00 Å R-free 0.200 |
| 7LS5 Cryo-EM structure of the Pre3-1 20S proteasome core particle Deposited 2021-02-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain K
1–198（198 aa）
Chain Y
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.74 Å |
| 7LS6 Cryo-EM structure of Pre-15S proteasome core particle assembly intermediate purified from Pre3-1 proteasome mutant (G34D) Deposited 2021-02-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric（15） Consistent with protein count |
Chain K
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.17 Å |
| 7LSX Cryo-EM structure of 13S proteasome core particle assembly intermediate purified from Pre3-1 proteasome mutant (G34D) Deposited 2021-02-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric（13） Consistent with protein count |
Chain K
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.61 Å |
| 7QO3 Structure of the 26S proteasome-Ubp6 complex in the si state (Core Particle and Lid) Deposited 2021-12-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 41 PDB declaration: 41-meric（41） Consistent with protein count |
Chain 4
1–198（198 aa）
Chain k
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.10 Å |
| 7QO5 26S proteasome Rpt1-RK -Ubp6-UbVS complex in the si state Deposited 2021-12-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric（49） Consistent with protein count |
Chain 4
1–198（198 aa）
Chain k
1–198（198 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.00 Å |
| 7TEJ Cryo-EM structure of the 20S Alpha 3 Deletion proteasome core particle Deposited 2022-01-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain K
1–198（198 aa）
Chain Y
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.74 Å |
| 7TEO Cryo-EM structure of the 20S Alpha 3 Deletion proteasome core particle in complex with FUB1 Deposited 2022-01-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain K
1–198（198 aa）
Chain Y
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.97 Å |
| 8BW1 Yeast 20S proteasome in complex with an engineered fellutamide derivative (C14QAL) Deposited 2022-12-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric（32） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 3 MYR MYRISTIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；100 mM MES, 20 mM MgAc2, 11% MPD
|
Resolution 3.25 Å R-free 0.212 |
| 8OHZ Yeast 20S proteasome in complex with a photoswitchable cepafungin derivative (transCep1) Deposited 2023-03-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 VOT (2~{S},3~{R})-2-[2-[4-[2-(4-ethylphenyl)hydrazinyl]phenyl]ethanoylamino]-~{N}-[(5~{S},8~{S},10~{S})-5-methyl-10-oxidanyl-2,7-bis(oxidanylidene)-1,6-diazacyclododec-8-yl]-3-oxidanyl-butanamide × 4 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.65 Å R-free 0.222 |
| 8OI1 Yeast 20S proteasome in complex with a photoswitchable cepafungin derivative (transCep4) Deposited 2023-03-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 11 VOX ~{N}-[(2~{S},3~{R})-1-[[(5~{S},8~{S},10~{S})-5-methyl-10-oxidanyl-2,7-bis(oxidanylidene)-1,6-diazacyclododec-8-yl]amino]-3-oxidanyl-1-oxidanylidene-butan-2-yl]-7-[4-[2-(4-methylphenyl)hydrazinyl]phenyl]heptanamide × 4 CL CHLORIDE ION × 2 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.95 Å R-free 0.224 |
| 8OLR Structure of yeast 20S proteasome in complex with the natural product beta-lactone inhibitor Cystargolide A Deposited 2023-03-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 VSZ Cystargolide A (bound) × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.208 |
| 8RHJ Yeast 20S proteasome in complex with a macrocyclic oxindole epoxyketone (compound 5) Deposited 2023-12-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 3.05 Å R-free 0.212 |
| 8RHK Yeast 20S proteasome in complex with a linear oxindole epoxyketone (compound 6) Deposited 2023-12-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.218 |
| 8RHL Yeast 20S proteasome in complex with a linear biarylether epoxyketone (compound 15a) Deposited 2023-12-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric（32） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 3.20 Å R-free 0.228 |
| 8RVL Proteasomal late precursor complex from pre1-1 Deposited 2024-02-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain K
1–198（198 aa）
Chain Y
1–198（198 aa）
|
Mutation：S142F Mutation：S142F | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；150 mM NaCl
50 mM Tris-HCl
cryo-EM vitrification conditions
Cryogen ETHANE；manual plunge freezing device purchased from 'Neptune Fluid Flow Systems'
|
Resolution 2.14 Å |
| 8RVO Proteasomal late precursor complex from pre1-1, state 1 Deposited 2024-02-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain K
1–198（198 aa）
Chain Y
1–198（198 aa）
|
Mutation：S142F Mutation：S142F | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；150 mM NaCl
50 mM Tris-HCl
cryo-EM vitrification conditions
Cryogen ETHANE；manual plunge freezing device purchased from 'Neptune Fluid Flow Systems'
|
Resolution 2.69 Å |
| 8RVP Proteasomal late precursor complex from pre1-1, state 2 Deposited 2024-02-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain K
1–198（198 aa）
Chain Y
1–198（198 aa）
|
Mutation：S142F Mutation：S142F | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；150 mM NaCl
50 mM Tris-HCl
cryo-EM vitrification conditions
Cryogen ETHANE；manual plunge freezing device purchased from 'Neptune Fluid Flow Systems'
|
Resolution 2.28 Å |
| 8RVQ 20S proteasome from pre1-1 Deposited 2024-02-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain K
1–198（198 aa）
Chain Y
1–198（198 aa）
|
Mutation：S142F Mutation：S142F | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；150 mM NaCl
50 mM Tris-HCl
cryo-EM vitrification conditions
Cryogen ETHANE；manual plunge freezing device purchased from 'Neptune Fluid Flow Systems'
|
Resolution 2.02 Å |
| 8T08 Preholo-Proteasome from Pre1-1 Pre4-1 Double Mutant Deposited 2023-05-31 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain K
1–198（198 aa）
Chain b
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8T0M Proteasome 20S core particle from Pre1-1 Pre4-1 Double mutant Deposited 2023-06-01 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain K
1–198（198 aa）
Chain Y
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 8U6Y Preholo-Proteasome from Beta 3 D205 deletion Deposited 2023-09-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain K
1–198（198 aa）
Chain b
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；Fluorinated Fos-Choline was added immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 8U7U Proteasome 20S Core Particle from Beta 3 D205 deletion Deposited 2023-09-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain K
1–198（198 aa）
Chain Y
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；Fluorinated Fos-Choline was added immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.16 Å |
| 9AW3 Yeast 20S proteasome soaked with MA9 crude extract Deposited 2024-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 SO4 SULFATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
|
Resolution 3.42 Å R-free 0.259 |
| 9AW5 Yeast 20S proteasome soaked with MA9 fraction E/F Deposited 2024-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | SO4 SULFATE ION × 61 MG MAGNESIUM ION × 8 A1A9B (10S,11R,12S,15S,18S)-15-(2-amino-2-oxoethyl)-10,11,23-trihydroxy-18-{[(3R)-3-methyl-2-oxopentanoyl]amino}-9,14,17-trioxo-N-[(1Z)-prop-1-en-1-yl]-8,13,16-triazatetracyclo[18.3.1.0(2,7).0(6,10)]tetracosa-1(24),2,4,6,20,22-hexaene-12-carboxamide × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
|
Resolution 3.44 Å R-free 0.252 |
| 9AW6 Yeast 20S proteasome soaked with MA9 fraction EF2 Deposited 2024-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | SO4 SULFATE ION × 58 A1A9B (10S,11R,12S,15S,18S)-15-(2-amino-2-oxoethyl)-10,11,23-trihydroxy-18-{[(3R)-3-methyl-2-oxopentanoyl]amino}-9,14,17-trioxo-N-[(1Z)-prop-1-en-1-yl]-8,13,16-triazatetracyclo[18.3.1.0(2,7).0(6,10)]tetracosa-1(24),2,4,6,20,22-hexaene-12-carboxamide × 6 MG MAGNESIUM ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
|
Resolution 3.44 Å R-free 0.235 |
| 9C98 Yeast 20S proteasome soaked with isolated TMC-86A Deposited 2024-06-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | SO4 SULFATE ION × 69 MG MAGNESIUM ION × 12 A1AU6 N-[(2S)-3-hydroxy-1-{[(4S)-1-hydroxy-2-(hydroxymethyl)-6-methyl-3-oxoheptan-4-yl]amino}-1-oxopropan-2-yl]butanamide × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.9；291 K；MES 100 mM pH 6.9, MPD 25% and magnesium acetate 20 mM
|
Resolution 3.04 Å R-free 0.221 |
| 9CGC Yeast 26S proteasome non-substrate-engaged (S1 state) Deposited 2024-06-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric（39） Consistent with protein count |
Chain 4
1–198（198 aa）
Chain g
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；30 mM HEPES pH7.4, 25 mM NaCl, 25 mM KCl, 3% (v/v) glycerol, 5 mM MgCl2
2 mM ATP and 0.5 mM TCEP, 0.02% NP-40.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.61 Å |
| 9D0T Proteasome core particle assembly intermediate Blm10:13S purified from Saccharomyces cerevisiae Deposited 2024-08-07 | Assembly 1 Insufficient information Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain K
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.84 Å |
| 9D35 Proteasome core particle assembly intermediate 5-alpha/3-beta/Ump1 purified from Saccharomyces cerevisiae. Deposited 2024-08-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric（9） Consistent with protein count |
Chain K
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.26 Å |
| 9D3I Proteasome core particle assembly intermediate 5-alpha/4-beta/Ump1 purified from Saccharomyces cerevisiae. Deposited 2024-08-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric（10） Consistent with protein count |
Chain K
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.11 Å |
| 9D4U Core particle assembly intermediate Capless 13S purified from Saccharomyces cerevisiae Deposited 2024-08-13 | Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: 11-meric（11） Consistent with protein count |
Chain K
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.55 Å |
| 9EY9 Yeast 20S proteasome in complex with a sybactin derivative (PheSyr) Deposited 2024-04-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 A1H71 sybactin derivative × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 3.10 Å R-free 0.241 |
| 9FRW Yeast 20S proteasome with human beta1i (1-51) Deposited 2024-06-19 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | CL CHLORIDE ION × 7 MG MAGNESIUM ION × 9 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.85 Å R-free 0.227 |
| 9FST Yeast 20S proteasome with human beta1i (1-51) in complex with epoxyketone inhibitor LU-001i Deposited 2024-06-21 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | CL CHLORIDE ION × 4 MG MAGNESIUM ION × 8 A1IF8 azanylidene-[2-[[(2S)-1-[(2S)-2-[[(2S)-1-[[(1S)-2-cyclohexyl-1-[(2R,3S,6R,7S)-3-methanoyl-2,6-dimethyl-6,7-bis(oxidanyl)-1,4-oxazepan-7-yl]ethyl]amino]-1-oxidanylidene-hexan-2-yl]carbamoyl]-4,4-bis(fluoranyl)pyrrolidin-1-yl]-1-oxidanylidene-propan-2-yl]amino]-2-oxidanylidene-ethyl]imino-azanium × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.75 Å R-free 0.245 |
| 9FSU Yeast 20S proteasome with human beta1i (1-51) in complex with epoxyketone inhibitor 16 Deposited 2024-06-21 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 SO4 SULFATE ION × 4 A1IFL (2S)-N-[(2S)-1-[[(1S)-2-cyclohexyl-1-[(2R,3S,6R,7S)-3-methanoyl-2,6-dimethyl-6,7-bis(oxidanyl)-1,4-oxazepan-7-yl]ethyl]amino]-3-(4-methoxyphenyl)-1-oxidanylidene-propan-2-yl]-2-(2-morpholin-4-ylethanoylamino)-4-oxidanyl-butanamide × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.75 Å R-free 0.245 |
| 9FSV Yeast 20S proteasome with human beta2i (1-53) in complex with epoxyketone inhibitor 16 Deposited 2024-06-22 | Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 A1IFL (2S)-N-[(2S)-1-[[(1S)-2-cyclohexyl-1-[(2R,3S,6R,7S)-3-methanoyl-2,6-dimethyl-6,7-bis(oxidanyl)-1,4-oxazepan-7-yl]ethyl]amino]-3-(4-methoxyphenyl)-1-oxidanylidene-propan-2-yl]-2-(2-morpholin-4-ylethanoylamino)-4-oxidanyl-butanamide × 4 SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.75 Å R-free 0.247 |
| 9FT0 Yeast 20S proteasome in complex with epoxyketone inhibitor 16 Deposited 2024-06-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 9 CL CHLORIDE ION × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 A1IFL (2S)-N-[(2S)-1-[[(1S)-2-cyclohexyl-1-[(2R,3S,6R,7S)-3-methanoyl-2,6-dimethyl-6,7-bis(oxidanyl)-1,4-oxazepan-7-yl]ethyl]amino]-3-(4-methoxyphenyl)-1-oxidanylidene-propan-2-yl]-2-(2-morpholin-4-ylethanoylamino)-4-oxidanyl-butanamide × 4 SO4 SULFATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.75 Å R-free 0.232 |
| 9FT1 Yeast 20S proteasome in complex with epoxyketone inhibitor 9 Deposited 2024-06-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 5 A1IFK tert-butyl 4-[2-[[(2S)-1-[[(2S)-1-[[(1S)-2-cyclohexyl-1-[(2R,3S,6R,7S)-3-methanoyl-2,6-dimethyl-6,7-bis(oxidanyl)-1,4-oxazepan-7-yl]ethyl]amino]-3-(4-methoxyphenyl)-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-3-phenylmethoxy-propan-2-yl]amino]-2-oxidanylidene-ethyl]piperazine-1-carboxylate × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.222 |
| 9GBK Blm10-20S proteasome complex from pre1-1 Deposited 2024-07-31 | Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric（29） Consistent with protein count |
Chain K
1–198（198 aa）
Chain Y
1–198（198 aa）
|
Mutation：S142F Mutation：S142F | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；150 mM NaCl
50 mM Tris-HCl
cryo-EM vitrification conditions
Cryogen ETHANE；manual plunge freezing device purchased from 'Neptune Fluid Flow Systems'
|
Resolution 2.39 Å |
| 9IWR 26S proteasome trimer Deposited 2024-07-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count |
Chain 4
1–198（198 aa）
Chain k
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8；YPD buffer
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 9.10 Å |
| 9QAF Yeast 20S proteasome mutant: beta5_T3M Deposited 2025-02-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.213 |
| 9QAI Yeast 20S proteasome mutant: beta5_T3M in complex with MG132 Deposited 2025-02-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 11 CL CHLORIDE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.205 |
| 9QB1 Yeast 20S proteasome mutant: beta5_T3M in complex with Bortezomib Deposited 2025-02-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.215 |
| 9QB4 Yeast 20S proteasome mutant: beta5_T3M in complex with Carfilzomib Deposited 2025-02-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.207 |
| 9QBE Yeast 20S proteasome mutant: beta5_T3M in complex with ONX0914 Deposited 2025-03-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.211 |
| 9QBI Yeast 20S proteasome mutant: beta5_G128V (b5-propeptide in trans) Deposited 2025-03-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | SO4 SULFATE ION × 2 MG MAGNESIUM ION × 3 CL CHLORIDE ION × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.215 |
| 9QBO Yeast 20S proteasome mutant: beta5_G128V (b5-propeptide in trans) in complex with MG132 Deposited 2025-03-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | SO4 SULFATE ION × 4 MG MAGNESIUM ION × 2 CL CHLORIDE ION × 4 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.212 |
| 9QBW Yeast 20S proteasome mutant: beta5_G128V (b5-propeptide in trans) in complex with Carfilzomib Deposited 2025-03-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 3 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 5 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.207 |
| 9QBY Yeast 20S proteasome mutant: beta5_G128V (b5-propeptide in trans) in complex with ONX0914 Deposited 2025-03-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 3 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.211 |
| 9QC9 Yeast 20S proteasome double mutant: beta5_F(-46)S / beta5_G128V Deposited 2025-03-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 6 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 8 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.60 Å R-free 0.224 |
| 9QCF Yeast 20S proteasome mutant: beta1_G128V Deposited 2025-03-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.90 Å R-free 0.214 |
| 9QCK Yeast 20S proteasome mutant: beta1_G128V (b1-propeptide deleted) Deposited 2025-03-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric（28） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 5 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.213 |
| 9QD2 Yeast 20S proteasome mutant: beta1_G128V (b1-propeptide deleted) in complex with MG132 Deposited 2025-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 4 CL CHLORIDE ION × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.214 |
| 9QDF Yeast 20S proteasome mutant: beta1_G128V (b1-propeptide deleted) in complex with Bortezomib Deposited 2025-03-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 4 CL CHLORIDE ION × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.70 Å R-free 0.217 |
| 9QDS Yeast 20S proteasome mutant: beta1_G128V (b1-propeptide deleted) in complex with Carfilzomib Deposited 2025-03-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric（34） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 4 CL CHLORIDE ION × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 2.80 Å R-free 0.211 |
| 9RL3 13S proteasome precursor complex Deposited 2025-06-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: 13-meric（13） Consistent with protein count |
Chain K
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；150 mM NaCl
50 mM Tris-HCl
cryo-EM vitrification conditions
Cryogen ETHANE；manual plunge freezing device purchased from "Neptune FLuid Flow Systems"
|
Resolution 3.31 Å |
| 9RLA 13S+Beta1 proteasome precursor complex Deposited 2025-06-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: 14-meric（14） Consistent with protein count |
Chain K
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；150 mM NaCl
50 mM Tris-HCl
cryo-EM vitrification conditions
Cryogen ETHANE；manual plunge freezing device purchased from "Neptune FLuid Flow Systems"
|
Resolution 3.16 Å |
| 9RLT dimerised 13S-13S+Beta5 proteasome precursor complexes Deposited 2025-06-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric（27） Consistent with protein count |
Chain K
1–198（198 aa）
Chain Y
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；150 mM NaCl
50 mM Tris-HCl
cryo-EM vitrification conditions
Cryogen ETHANE；manual plunge freezing device purchased from "Neptune FLuid Flow Systems"
|
Resolution 3.09 Å |
| 9RLZ 15S proteasome precursor complex Deposited 2025-06-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: 16-meric（16） Consistent with protein count |
Chain K
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；150 mM NaCl
50 mM Tris-HCl
cryo-EM vitrification conditions
Cryogen ETHANE；manual plunge freezing device purchased from "Neptune FLuid Flow Systems"
|
Resolution 3.12 Å |
| 9RM0 13S+Beta5+Beta6 proteasome precursor complex Deposited 2025-06-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: 15-meric（15） Consistent with protein count |
Chain K
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；150 mM NaCl
50 mM Tris-HCl
cryo-EM vitrification conditions
Cryogen ETHANE；manual plunge freezing device purchased from "Neptune FLuid Flow Systems"
|
Resolution 3.29 Å |
| 9RM1 13S+Beta1+Beta5 proteasome precursor complex Deposited 2025-06-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: 15-meric（15） Consistent with protein count |
Chain K
1–198（198 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；150 mM NaCl
50 mM Tris-HCl
cryo-EM vitrification conditions
Cryogen ETHANE；manual plunge freezing device purchased from "Neptune FLuid Flow Systems"
|
Resolution 4.11 Å |
| 9S78 Yeast 20S Proteasome in Complex with Tellurophene-Tagged Carfilzomib Deposited 2025-08-04 | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric（32） Consistent with protein count |
Chain J
1–198（198 aa）
Chain X
1–198（198 aa）
|
Not recorded | MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
|
Resolution 3.15 Å R-free 0.223 |