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7o2l

Yeast 20S proteasome in complex with the covalently bound inhibitor b-lactone (2R,3S)-3-isopropyl-4-oxo-2-oxetane-carboxylate (IOC)

Method: X-RAY DIFFRACTION Dmax: 194.9 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

HLJ1_G0039880.mRNA.1.CDS.1

OrganismNot specified

UniProt A0A6L1BIF8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain A； UniProt 1–250 Chain O； UniProt 1–250 Not recorded BJ4_G0021480.mRNA.1.CDS.1 × 2 （A0A6A5PXC6） HLJ1_G0048980.mRNA.1.CDS.1 × 2 （A0A6A5Q273） 20S proteasome × 2 （P32379） BJ4_G0043800.mRNA.1.CDS.1 × 2 （A0A6A5PTH4） Probable proteasome subunit alpha type-7 × 2 （A0A6A5Q4M4） BJ4_G0020160.mRNA.1.CDS.1 × 2 （A0A6A5PYC9） Proteasome endopeptidase complex × 2 （A0A6A5Q449） Proteasome endopeptidase complex × 2 （A0A6L0YA22） Proteasome subunit beta × 2 （A0A6A5Q0W2） Proteasome subunit beta type-5 × 2 （A0A6V8RU78） Proteasome endopeptidase complex × 2 （A0A6A5Q0P3） Proteasome subunit beta type-7 × 2 （P30657） Proteasome endopeptidase complex × 2 （A0A6L0ZSP2） MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 V08 (2 {R},3 {S})-3-methanoyl-4-methyl-2-hydroxy-pentanoic acid × 6 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES Resolution 3.00 Å R-free 0.216

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7O2L Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3D29 Proteasome Inhibition by Fellutamide B Deposited 2008-05-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain A 1–250（250 aa）
Chain O 1–250（250 aa）
Not recorded HXD (3R)-3-HYDROXYDODECANOIC ACID × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；10 % MPD; 0,1 M MES, 30 mM MgAc2, pH 6,8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.60 Å R-free 0.266
7QO6 26S proteasome Rpt1-RK -Ubp6-UbVS complex in the s2 state Deposited 2021-12-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric
Chain B 1–250（250 aa）
Chain b 1–250（250 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
9AW7 Yeast 20S proteasome soaked with isolated TMC-95B Deposited 2024-03-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain A 1–250（250 aa）
Chain O 1–250（250 aa）
Not recorded SO4 SULFATE ION × 85 MG MAGNESIUM ION × 12 A1AHA TMC-95B × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 ACY ACETIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
Resolution 2.91 Å R-free 0.232
9C97 Yeast 20S proteasome soaked with BRA-346 fraction Deposited 2024-06-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain A 1–250（250 aa）
Chain O 1–250（250 aa）
Not recorded SO4 SULFATE ION × 44 MG MAGNESIUM ION × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；291 K；MES 100 mM pH 6.9, MPD 25% and magnesium acetate 20 mM
Resolution 3.33 Å R-free 0.252

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name A0A6L1BIF8_YEASX
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–250； UniProt 1–250 Author chain O； PDBConstruct 1–250； UniProt 1–250

BJ4_G0021480.mRNA.1.CDS.1

OrganismNot specified

UniProt A0A6A5PXC6

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain B； UniProt 1–258 Chain P； UniProt 1–258 Not recorded HLJ1_G0039880.mRNA.1.CDS.1 × 2 （A0A6L1BIF8） HLJ1_G0048980.mRNA.1.CDS.1 × 2 （A0A6A5Q273） 20S proteasome × 2 （P32379） BJ4_G0043800.mRNA.1.CDS.1 × 2 （A0A6A5PTH4） Probable proteasome subunit alpha type-7 × 2 （A0A6A5Q4M4） BJ4_G0020160.mRNA.1.CDS.1 × 2 （A0A6A5PYC9） Proteasome endopeptidase complex × 2 （A0A6A5Q449） Proteasome endopeptidase complex × 2 （A0A6L0YA22） Proteasome subunit beta × 2 （A0A6A5Q0W2） Proteasome subunit beta type-5 × 2 （A0A6V8RU78） Proteasome endopeptidase complex × 2 （A0A6A5Q0P3） Proteasome subunit beta type-7 × 2 （P30657） Proteasome endopeptidase complex × 2 （A0A6L0ZSP2） MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 V08 (2 {R},3 {S})-3-methanoyl-4-methyl-2-hydroxy-pentanoic acid × 6 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES Resolution 3.00 Å R-free 0.216

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7O2L Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3D29 Proteasome Inhibition by Fellutamide B Deposited 2008-05-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain B 2–245（244 aa）
Chain P 2–245（244 aa）
Not recorded HXD (3R)-3-HYDROXYDODECANOIC ACID × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；10 % MPD; 0,1 M MES, 30 mM MgAc2, pH 6,8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.60 Å R-free 0.266
7QO6 26S proteasome Rpt1-RK -Ubp6-UbVS complex in the s2 state Deposited 2021-12-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric
Chain C 1–258（258 aa）
Chain c 1–258（258 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
9AW7 Yeast 20S proteasome soaked with isolated TMC-95B Deposited 2024-03-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain B 1–258（258 aa）
Chain P 1–258（258 aa）
Not recorded SO4 SULFATE ION × 85 MG MAGNESIUM ION × 12 A1AHA TMC-95B × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 ACY ACETIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
Resolution 2.91 Å R-free 0.232
9C97 Yeast 20S proteasome soaked with BRA-346 fraction Deposited 2024-06-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain B 1–258（258 aa）
Chain P 1–258（258 aa）
Not recorded SO4 SULFATE ION × 44 MG MAGNESIUM ION × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；291 K；MES 100 mM pH 6.9, MPD 25% and magnesium acetate 20 mM
Resolution 3.33 Å R-free 0.252

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name A0A6A5PXC6_YEASX
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–258； UniProt 1–258 Author chain P； PDBConstruct 1–258； UniProt 1–258

HLJ1_G0048980.mRNA.1.CDS.1

OrganismNot specified

UniProt A0A6A5Q273

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain C； UniProt 1–254 Chain Q； UniProt 1–254 Not recorded HLJ1_G0039880.mRNA.1.CDS.1 × 2 （A0A6L1BIF8） BJ4_G0021480.mRNA.1.CDS.1 × 2 （A0A6A5PXC6） 20S proteasome × 2 （P32379） BJ4_G0043800.mRNA.1.CDS.1 × 2 （A0A6A5PTH4） Probable proteasome subunit alpha type-7 × 2 （A0A6A5Q4M4） BJ4_G0020160.mRNA.1.CDS.1 × 2 （A0A6A5PYC9） Proteasome endopeptidase complex × 2 （A0A6A5Q449） Proteasome endopeptidase complex × 2 （A0A6L0YA22） Proteasome subunit beta × 2 （A0A6A5Q0W2） Proteasome subunit beta type-5 × 2 （A0A6V8RU78） Proteasome endopeptidase complex × 2 （A0A6A5Q0P3） Proteasome subunit beta type-7 × 2 （P30657） Proteasome endopeptidase complex × 2 （A0A6L0ZSP2） MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 V08 (2 {R},3 {S})-3-methanoyl-4-methyl-2-hydroxy-pentanoic acid × 6 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES Resolution 3.00 Å R-free 0.216

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7O2L Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3D29 Proteasome Inhibition by Fellutamide B Deposited 2008-05-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain C 3–243（241 aa）
Chain Q 3–243（241 aa）
Not recorded HXD (3R)-3-HYDROXYDODECANOIC ACID × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；10 % MPD; 0,1 M MES, 30 mM MgAc2, pH 6,8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.60 Å R-free 0.266
7QO6 26S proteasome Rpt1-RK -Ubp6-UbVS complex in the s2 state Deposited 2021-12-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric
Chain D 1–254（254 aa）
Chain d 1–254（254 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
9AW7 Yeast 20S proteasome soaked with isolated TMC-95B Deposited 2024-03-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain C 1–254（254 aa）
Chain Q 1–254（254 aa）
Not recorded SO4 SULFATE ION × 85 MG MAGNESIUM ION × 12 A1AHA TMC-95B × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 ACY ACETIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
Resolution 2.91 Å R-free 0.232
9C97 Yeast 20S proteasome soaked with BRA-346 fraction Deposited 2024-06-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain C 1–254（254 aa）
Chain Q 1–254（254 aa）
Not recorded SO4 SULFATE ION × 44 MG MAGNESIUM ION × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；291 K；MES 100 mM pH 6.9, MPD 25% and magnesium acetate 20 mM
Resolution 3.33 Å R-free 0.252

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name A0A6A5Q273_YEASX
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 1–254； UniProt 1–254 Author chain Q； PDBConstruct 1–254； UniProt 1–254

20S proteasome

OrganismNot specified

UniProt P32379

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain D； UniProt 1–260 Chain R； UniProt 1–260 Not recorded HLJ1_G0039880.mRNA.1.CDS.1 × 2 （A0A6L1BIF8） BJ4_G0021480.mRNA.1.CDS.1 × 2 （A0A6A5PXC6） HLJ1_G0048980.mRNA.1.CDS.1 × 2 （A0A6A5Q273） BJ4_G0043800.mRNA.1.CDS.1 × 2 （A0A6A5PTH4） Probable proteasome subunit alpha type-7 × 2 （A0A6A5Q4M4） BJ4_G0020160.mRNA.1.CDS.1 × 2 （A0A6A5PYC9） Proteasome endopeptidase complex × 2 （A0A6A5Q449） Proteasome endopeptidase complex × 2 （A0A6L0YA22） Proteasome subunit beta × 2 （A0A6A5Q0W2） Proteasome subunit beta type-5 × 2 （A0A6V8RU78） Proteasome endopeptidase complex × 2 （A0A6A5Q0P3） Proteasome subunit beta type-7 × 2 （P30657） Proteasome endopeptidase complex × 2 （A0A6L0ZSP2） MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 V08 (2 {R},3 {S})-3-methanoyl-4-methyl-2-hydroxy-pentanoic acid × 6 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES Resolution 3.00 Å R-free 0.216

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7O2L Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1FNT CRYSTAL STRUCTURE OF THE 20S PROTEASOME FROM YEAST IN COMPLEX WITH THE PROTEASOME ACTIVATOR PA26 FROM TRYPANOSOME BRUCEI AT 3.2 ANGSTROMS RESOLUTION Deposited 2000-08-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain E 1–260（260 aa）
Chain S 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 14 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；0.1 M NaHEPES, 40% MPD, 0.2 M NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP
Resolution 3.20 Å R-free 0.325
1G0U A GATED CHANNEL INTO THE PROTEASOME CORE PARTICLE Deposited 2000-10-09 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–250（250 aa）
Chain R 1–250（250 aa）
Not recorded MG MAGNESIUM ION × 20 X-RAY DIFFRACTION
X-ray crystallization conditions hanging drop vapour-diffusion；pH 6.7；297 K；22mM Magnesium acetate, 4% Dimethylsulfoxide, 100mM morpholinoethanesulfonic acid, 11% methylpentanediol, pH 6.7, hanging drop vapour-diffusion, temperature 297K
Resolution 2.40 Å R-free 0.303
1G65 Crystal structure of epoxomicin:20s proteasome reveals a molecular basis for selectivity of alpha,beta-epoxyketone proteasome inhibitors Deposited 2000-11-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 9–250（242 aa）
Chain R 9–250（242 aa）
Not recorded MG MAGNESIUM ION × 10 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；0.1M Mes, pH 6.8 11% MPD 25mM MgAc2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.25 Å R-free 0.336
1JD2 Crystal Structure of the yeast 20S Proteasome:TMC-95A complex: A non-covalent Proteasome Inhibitor Deposited 2001-06-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 9–250（242 aa）
Chain Y 9–250（242 aa）
Not recorded MG MAGNESIUM ION × 10 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；MPD, MES, DMSO, magnesium Acetate, pH 6.4, VAPOR DIFFUSION, HANGING DROP at 293K
Resolution 3.00 Å R-free 0.336
1RYP CRYSTAL STRUCTURE OF THE 20S PROTEASOME FROM YEAST AT 2.4 ANGSTROMS RESOLUTION Deposited 1997-02-26 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain E 9–250（242 aa）
Chain S 9–250（242 aa）
Mutation：CHAINS H, V, T1A, CHAIN L, Z, K33R Mutation：CHAINS H, V, T1A, CHAIN L, Z, K33R MG MAGNESIUM ION × 20 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.9；pH 6.9
Resolution 1.90 Å R-free 0.330
1Z7Q Crystal structure of the 20s proteasome from yeast in complex with the proteasome activator PA26 from Trypanosome brucei at 3.2 angstroms resolution Deposited 2005-03-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain E 1–260（260 aa）
Chain S 1–260（260 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.1 M NaHEPES, 40% MPD, 0.2 M NACL, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 3.22 Å R-free 0.308
2F16 Crystal structure of the yeast 20S proteasome in complex with bortezomib Deposited 2005-11-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa）
Chain R 9–250（242 aa）
Not recorded BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；12 % MPD 28mM MgAc2 50mM MES-NaOH, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.264
2FAK Crystal structure of Salinosporamide A in complex with the yeast 20S proteasome Deposited 2005-12-07 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa）
Chain R 9–250（242 aa）
Not recorded SA1 (3AR,6R,6AS)-6-((S)-((S)-CYCLOHEX-2-ENYL)(HYDROXY)METHYL)-6A-METHYL-4-OXO-HEXAHYDRO-2H-FURO[3,2-C]PYRROLE-6-CARBALDEHYDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；Crystals appeared after one week; Crystallization conditions: 40mg/ml yeast 20S proteasome 0.1M MES; 28mM MgAc2, 12% MPD 6h crystal soak with 10mM SalA, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.254
2GPL TMC-95 based biphenyl-ether macrocycles: specific proteasome inhibitors Deposited 2006-04-18 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa）
Chain R 9–250（242 aa）
Not recorded BIQ BENZYL [12-(2-AMINO-2-OXOETHYL)-4-NITRO-10,13-DIOXO-15-[(PROPYLAMINO)CARBONYL]-2-OXA-11,14-DIAZATRICYCLO[15 .2.2.1~3,7~]DOCOSA-1(19),3(22),4,6,17,20-HEXAEN-9-YL]CARBAMATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；20mM MgAc2; 0.1M MES, 12% MPD; crystals appeared within 7 days; crystal size 0.2 x 0.2 x 0.6 mm3, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.81 Å R-free 0.241
2Z5C Crystal Structure of a Novel Chaperone Complex for Yeast 20S Proteasome Assembly Deposited 2007-07-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1–260（260 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.1M Tris-HCl, 8%(v/v) ethyleneglycol, 12%(w/v) PEG 8000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.90 Å R-free 0.283
2Z5C Crystal Structure of a Novel Chaperone Complex for Yeast 20S Proteasome Assembly Deposited 2007-07-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain F 1–260（260 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.1M Tris-HCl, 8%(v/v) ethyleneglycol, 12%(w/v) PEG 8000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.90 Å R-free 0.283
2ZCY yeast 20S proteasome:syringolin A-complex Deposited 2007-11-15 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded SRG (2S)-2-[[(2S)-1-[[(5S,8S,9E)-2,7-dioxo-5-propan-2-yl-1,6-diazacyclododeca-3,9-dien-8-yl]amino]-3-methyl-1-oxo-butan-2-yl]carbamoylamino]-3-methyl-butanoic acid × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；294 K；0.1M MES, 12% MPD, 30mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 294K
Resolution 2.90 Å R-free 0.245
3BDM yeast 20S proteasome:glidobactin A-complex Deposited 2007-11-15 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded GDT (2E,4E)-N-[(2S,3R)-3-hydroxy-1-[[(3Z,5S,8S,10S)-10-hydroxy-5-methyl-2,7-dioxo-1,6-diazacyclododec-3-en-8-yl]amino]-1-ox obutan-2-yl]dodeca-2,4-dienamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；294 K；100mM Mes, 14% MPD, 30mM MgAc2, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K
Resolution 2.70 Å R-free 0.262
3DY3 Crystal structure of yeast 20S proteasome in complex with the epimer form of spirolactacystin Deposited 2008-07-25 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa） Fragment：UNP residues 9-250
Chain R 9–250（242 aa） Fragment：UNP residues 9-250
Not recorded SLR (3R,4R)-3-hydroxy-2-[(1S)-1-hydroxy-2-methylpropyl]-4-methyl-5-oxo-D-proline × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；0.1 M MES, 10 % MPD, 20 mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.81 Å R-free 0.245
3DY4 Crystal structure of yeast 20S proteasome in complex with spirolactacystin Deposited 2008-07-25 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa） Fragment：UNP residues 9-250
Chain R 9–250（242 aa） Fragment：UNP residues 9-250
Not recorded SLA Omuralide, open form × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；0.1 M MES, 10 % MPD, 20 mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.80 Å R-free 0.256
3E47 Crystal Structure of the Yeast 20S Proteasome in Complex with Homobelactosin C Deposited 2008-08-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa） Fragment：UNP residues 9-250
Chain R 9–250（242 aa） Fragment：UNP residues 9-250
Not recorded ESY benzyl N-[(benzyloxy)carbonyl]-L-alanyl-N~6~-[(2R,3S,4S)-3-formyl-2-hydroxy-4-methylhexanoyl]-L-lysinate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；20% MPD, 0.1M MES, 30mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 3.00 Å R-free 0.245
3GPJ Crystal structure of the yeast 20S proteasome in complex with syringolin B Deposited 2009-03-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa） Fragment：sequence database residues 9-250
Chain R 9–250（242 aa） Fragment：sequence database residues 9-250
Not recorded SY2 N-{[(1S)-2-methyl-1-{[(5S,8S)-5-(1-methylethyl)-2,7-dioxo-1,6-diazacyclododec-3-en-8-yl]carbamoyl}propyl]carbamoyl}-L-valine × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；12% MPD, 0.1M MES, pH 6.7, 20mM MgAc2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.264
3GPT Crystal structure of the yeast 20S proteasome in complex with Salinosporamide derivatives: slow substrate ligand Deposited 2009-03-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa） Fragment：sequence database residues 9-250
Chain R 9–250（242 aa） Fragment：sequence database residues 9-250
Not recorded GPT (2R,3S,4R)-2-[(S)-(1S)-cyclohex-2-en-1-yl(hydroxy)methyl]-4-(2-fluoroethyl)-3-hydroxy-3-methyl-5-oxopyrrolidine-2-carbaldehyde × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；12% MPD, 0.1M MES, 20mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.41 Å R-free 0.245
3GPW Crystal structure of the yeast 20S proteasome in complex with Salinosporamide derivatives: irreversible inhibitor ligand Deposited 2009-03-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa） Fragment：sequence database residues 9-250
Chain R 9–250（242 aa） Fragment：sequence database residues 9-250
Not recorded SA1 (3AR,6R,6AS)-6-((S)-((S)-CYCLOHEX-2-ENYL)(HYDROXY)METHYL)-6A-METHYL-4-OXO-HEXAHYDRO-2H-FURO[3,2-C]PYRROLE-6-CARBALDEHYDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.7；12% MPD, 0.1M MES, 20mM MgAc2, pH 6.7, VAPOR DIFFUSION, HANGING DROP
Resolution 2.50 Å R-free 0.255
3HYE Crystal structure of 20S proteasome in complex with hydroxylated salinosporamide Deposited 2009-06-22 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa）
Chain R 9–250（242 aa）
Not recorded HYE (2R,3S,4R)-2-[(S)-(1S)-cyclohex-2-en-1-yl(hydroxy)methyl]-3-hydroxy-4-(2-hydroxyethyl)-3-methyl-5-oxopyrrolidine-2-carbaldehyde × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；Crystals grew within one week under 0.1M MES/HCl,pH 6.6, 30mM MgAc2 and 15% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å R-free 0.242
3JCO Structure of yeast 26S proteasome in M1 state derived from Titan dataset Deposited 2016-01-06 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain E 1–260（260 aa）
Chain e 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 50mM Tris pH 7.5, 100mM NaCl, 5mM MgCl2, 2mM ATP；pH 7.5；50mM Tris pH 7.5, 100mM NaCl, 5mM MgCl2, 2mM ATP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.80 Å
3JCP Structure of yeast 26S proteasome in M2 state derived from Titan dataset Deposited 2016-01-06 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain E 1–260（260 aa）
Chain e 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 50mM Tris pH 7.5, 100mM NaCl, 5mM MgCl2, 2mM ATP；pH 7.5；50mM Tris pH 7.5, 100mM NaCl, 5mM MgCl2, 2mM ATP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
3MG0 Structure of yeast 20S proteasome with bortezomib Deposited 2010-04-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa）
Chain R 9–250（242 aa）
Not recorded BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, pH 7.0, vapor diffusion, hanging drop, temperature 293K
Resolution 2.68 Å R-free 0.253
3MG4 Structure of yeast 20S proteasome with Compound 1 Deposited 2010-04-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa）
Chain R 9–250（242 aa）
Not recorded MG MAGNESIUM ION × 10 LXT (2S)-2-amino-N-[(1S)-1-({(1S)-1-[(4-methylbenzyl)carbamoyl]-3-phenylpropyl}carbamoyl)-3-phenylpropyl]-4-phenylbutanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, pH 7.0, vapor diffusion, hanging drop, temperature 293K
Resolution 3.11 Å R-free 0.250
3MG6 Structure of yeast 20S open-gate proteasome with Compound 6 Deposited 2010-04-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–250（250 aa）
Chain R 1–250（250 aa）
Not recorded MG MAGNESIUM ION × 10 LZT N~2~-{[(1S)-6-methoxy-3-oxo-2,3-dihydro-1H-inden-1-yl]acetyl}-N-{(1S)-1-[(4-methylbenzyl)carbamoyl]-3-phenylpropyl}-L-threoninamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, pH 7.0, vapor diffusion, hanging drop, temperature 293K
Resolution 2.60 Å R-free 0.249
3MG7 Structure of yeast 20S open-gate proteasome with Compound 8 Deposited 2010-04-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–250（250 aa）
Chain R 1–250（250 aa）
Not recorded MG MAGNESIUM ION × 10 L2T 4-(benzyloxy)-N-[(1S,2R)-2-hydroxy-1-({(1S)-1-[(2-methylbenzyl)carbamoyl]-3-phenylpropyl}carbamoyl)propyl]benzamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, pH 7.0, vapor diffusion, hanging drop, temperature 293K
Resolution 2.78 Å R-free 0.259
3MG8 Structure of yeast 20S open-gate proteasome with Compound 16 Deposited 2010-04-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–250（250 aa）
Chain R 1–250（250 aa）
Not recorded MG MAGNESIUM ION × 10 L3T N-(2,2-dimethylpropyl)-N~2~-[1H-indol-3-yl(oxo)acetyl]-L-asparaginyl-N-(2-methylbenzyl)-3-pyridin-4-yl-L-alaninamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, pH 7.0, vapor diffusion, hanging drop, temperature 293K
Resolution 2.59 Å R-free 0.252
3NZJ Crystal structure of yeast 20S proteasome in complex with ligand 2a Deposited 2010-07-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；293 K；1000 mM MES / HCl, 6.8, 12% MPD, 20 mM MgAc2, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.40 Å R-free 0.249
3NZW Crystal structure of the yeast 20S proteasome in complex with 2b Deposited 2010-07-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；100 mM MES/HCl, 6.8, 20mM MgAc2, 12% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å R-free 0.240
3NZX Crystal structure of the yeast 20S proteasome in complex with ligand 2c Deposited 2010-07-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；100 mM MES/Hcl, pH 6.8, 20 mM MGAc2, 12 % MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.248
3OEU Structure of yeast 20S open-gate proteasome with Compound 24 Deposited 2010-08-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 OEU N-{(2S)-1-[(2-chlorobenzyl)amino]-1-oxo-4-phenylbutan-2-yl}-N~2~-[3-(2-methylphenyl)propanoyl]-L-threoninamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, pH 7.0, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, vapor diffusion, hanging drop, temperature 293K
Resolution 2.60 Å R-free 0.249
3OEV Structure of yeast 20S open-gate proteasome with Compound 25 Deposited 2010-08-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 3OE 4-(benzyloxy)-N-[(2S,3R)-3-hydroxy-1-{[(2S)-1-{[(3-methylthiophen-2-yl)methyl]amino}-1-oxo-4-phenylbutan-2-yl]amino}-1-oxobutan-2-yl]benzamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, pH 7.0, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, vapor diffusion, hanging drop, temperature 293K
Resolution 2.85 Å R-free 0.257
3OKJ Alpha-keto-aldehyde binding mechanism reveals a novel lead structure motif for proteasome inhibition Deposited 2010-08-25 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa） Fragment：sequence database residues 9-250
Chain R 9–250（242 aa） Fragment：sequence database residues 9-250
Not recorded EP9 N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S,3S)-3-hydroxy-1-(4-hydroxyphenyl)-4-oxobutan-2-yl]-L-leucinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；293 K；Crystalls appeared during one week using 100mM MES, 20mM MG2Ac, 12% MPD, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.259
3SDI Structure of yeast 20S open-gate proteasome with Compound 20 Deposited 2011-06-09 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 20 3SD N~4~-(2,2-dimethylpropyl)-N~1~-{(2S)-1-[(4-methylbenzyl)amino]-1-oxo-4-phenylbutan-2-yl}-N~2~-[(5-methyl-1,2-oxazol-3-yl)carbonyl]-L-aspartamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, pH 7.0, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, vapor diffusion, hanging drop, temperature 293K
Resolution 2.65 Å R-free 0.253
3SDK Structure of yeast 20S open-gate proteasome with Compound 34 Deposited 2011-06-09 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 P3N N-[(2S)-3-(3-tert-butyl-1,2,4-oxadiazol-5-yl)-1-({(2S)-1-[(4-methylbenzyl)amino]-1-oxo-4-phenylbutan-2-yl}amino)-1-oxopropan-2-yl]-5-methyl-1,2-oxazole-3-carboxamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, pH 7.0, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, vapor diffusion, hanging drop, temperature 293K
Resolution 2.70 Å R-free 0.259
3SHJ Proteasome in complex with hydroxyurea derivative HU10 Deposited 2011-06-16 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa）
Chain R 9–250（242 aa）
Not recorded MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 H10 1-hydroxy-1-[(2R)-4-{3-[(3S,5S,7S)-tricyclo[3.3.1.1~3,7~]dec-1-yloxy]phenyl}but-3-yn-2-yl]urea × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20 mM magnesium acetate, 11% PEG, 100 mM MES/NaOH, pH 7.0, crystals grow to their final size within a week, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.266
3TDD Crystal structure of yeast CP in complex with Belactosin C Deposited 2011-08-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa）
Chain R 9–250（242 aa）
Not recorded BFO benzyl N-[(naphthalen-2-ylmethoxy)carbonyl]-L-alanyl-N~5~-[(2R,3S,4S)-3-formyl-2-hydroxy-4-methylhexanoyl]-L-ornithinate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；100 mM MES, 12% MPD, 20 mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.236
3UN4 Yeast 20S proteasome in complex with PR-957 (morpholine) Deposited 2011-11-15 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；100 mM MES, 12% MPD, 20 mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.40 Å R-free 0.219
3UN8 Yeast 20S proteasome in complex with PR-957 (epoxide) Deposited 2011-11-15 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded 049 2-(acetylamino)-4,5-anhydro-1,2-dideoxy-4-methyl-1-phenyl-D-xylitol × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；100 mM MES, 12% MPD, 20 mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.240
3WXR Yeast 20S proteasome with a mutation of alpha7 subunit Deposited 2014-08-06 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain E 1–260（260 aa）
Chain S 1–260（260 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；14% MPD, 0.1M MES, 50mM Magnesium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.15 Å R-free 0.242
4CR2 Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome Deposited 2014-02-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 33 PDB declaration: 33-meric
Chain E 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.1
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- HOMEMADE PLUNGER,
Resolution 7.70 Å
4CR3 Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome Deposited 2014-02-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 33 PDB declaration: 33-meric
Chain E 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.1
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- HOMEMADE PLUNGER,
Resolution 9.30 Å
4CR4 Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome Deposited 2014-02-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 33 PDB declaration: 33-meric
Chain E 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.1
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- HOMEMADE PLUNGER,
Resolution 8.80 Å
4EU2 Crystal structure of 20s proteasome with novel inhibitor K-7174 Deposited 2012-04-25 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain E 9–250（242 aa） Fragment：UNP RESIDUES 9-250
Chain S 9–250（242 aa） Fragment：UNP RESIDUES 9-250
Not recorded WPI 1,4-bis[(4E)-5-(3,4,5-trimethoxyphenyl)pent-4-en-1-yl]-1,4-diazepane × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；293 K；4.5% (v/v) 2-methyl1-2,4-pentanediol(MPD), 36mM magnesium acetate, 90mM MES pH7.2, 10%(v/v) DMSO, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.51 Å R-free 0.255
4FZC 20S yeast proteasome in complex with cepafungin I Deposited 2012-07-06 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain D 9–250（242 aa）
Chain R 9–250（242 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；100 mM MES; 20 mM MgAc2; 12% MPD, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.244
4FZG 20S yeast proteasome in complex with glidobactin Deposited 2012-07-06 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain D 9–250（242 aa）
Chain R 9–250（242 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；100 mM MES; 20 mM MgAc2; 12% , pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.00 Å R-free 0.226
4G4S Structure of Proteasome-Pba1-Pba2 Complex Deposited 2012-07-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain E 1–260（260 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 16 LDZ N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S)-4-methyl-1-oxopentan-2-yl]-L-leucinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；277 K；0.1 M sodium citrate, 18-20% PEG 3000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 2.49 Å R-free 0.232
4GK7 yeast 20S proteasome in complex with the Syringolin-Glidobactin chimera Deposited 2012-08-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 9–250（242 aa）
Chain R 9–250（242 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；0.1M MES, 20mM MgAc2; 12% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.241
4HNP Crystal structure of yeast 20S proteasome in complex with vinylketone carmaphycin analogue VNK1 Deposited 2012-10-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa）
Chain R 9–250（242 aa）
Not recorded ONK N-hexanoyl-L-valyl-N~1~-[(3S,4S)-3-hydroxy-2,6-dimethylhept-1-en-4-yl]-N~5~,N~5~-dimethyl-L-glutamamide × 2 VNK N-hexanoyl-L-valyl-N~1~-[(3S,4S)-3-hydroxy-2,6-dimethylheptan-4-yl]-N~5~,N~5~-dimethyl-L-glutamamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.5；297 K；30 mM of magnesium acetate, 100 mM of MES (pH 7.2) and 12% of MPD, vapor diffusion, temperature 297K
Resolution 2.80 Å R-free 0.239
4HRC Crystal structure of yeast 20S proteasome in complex with epoxyketone carmaphycin analogue 3 Deposited 2012-10-27 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa）
Chain R 9–250（242 aa）
Not recorded OV2 N-hexanoyl-L-valyl-N~1~-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-N~5~,N~5~-dimethyl-L-glutamamide × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.5；297 K；30 mM of magnesium acetate, 100 mM of MES (pH 7.2) and 12% of MPD, vapor diffusion, temperature 297K
Resolution 2.80 Å R-free 0.243
4HRD Crystal structure of yeast 20S proteasome in complex with the natural product carmaphycin A Deposited 2012-10-27 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa）
Chain R 9–250（242 aa）
Not recorded OV1 N-[(2S)-1-({(2S)-1-{[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]amino}-4-[(R)-methylsulfinyl]-1-oxobutan-2-yl}amino)-3-methyl-1-oxobutan-2-yl]hexanamide × 6 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；297 K；30 mM of magnesium acetate, 100 mM of MES (pH 7.2) and 12% of MPD, vapor diffusion, temperature 297K
Resolution 2.80 Å R-free 0.239
4INR Yeast 20S proteasome in complex with the vinyl sulfone LU102 Deposited 2013-01-06 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded 1G1 N3Phe-Leu-Leu-Phe(4-NH2CH2)-methyl vinyl sulfone, bound form × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM magnesium acetate, 0.1 M MES, 12% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.231
4INT Yeast 20S proteasome in complex with the vinyl sulfone LU122 Deposited 2013-01-06 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded 1G5 HMB-Val-Ser-Phe(4-NH2CH2)-methyl vinyl sulfone, bound form × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM magnesium acetate, 0.1 M MES, 12% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.90 Å R-free 0.230
4INU Yeast 20S proteasome in complex with the vinyl sulfone LU112 Deposited 2013-01-06 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded 1G6 N3Phe-Phe(4-NH2CH2)-Leu-Phe(4-NH2CH2)-methyl vinyl sulfone, bound form × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；293 K；20 mM magnesium acetate, 0.1 M MES, 12% MPD, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.10 Å R-free 0.238
4J70 Yeast 20S proteasome in complex with the belactosin derivative 3e Deposited 2013-02-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded 1KR benzyl [(2S)-1-({(2R)-1-[(1S,2S)-2-{[(2R,3S,4S)-3-formyl-2-hydroxy-4-methylhexanoyl]amino}cyclopropyl]-4-phenylbutan-2-yl}amino)-1-oxopropan-2-yl]carbamate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；293 K；20 mM magnesium acetate, 0.1 M MES, 12% MPD, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.224
4JSQ Yeast 20S proteasome in complex with the dimerized linear mimetic of TMC-95A - yCP:4e Deposited 2013-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mM MgAc2, 0.1M MES, 12% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.241
4JSU Yeast 20S proteasome in complex with the dimerized linear mimetic of TMC-95A - yCP:3a Deposited 2013-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mM MgAc2, 0.1M MES, 12% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.90 Å R-free 0.227
4JT0 Yeast 20S proteasome in complex with the dimerized linear mimetic of TMC-95A - yCP:4a Deposited 2013-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 0L1 hexanedioic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mM MgAc2, 0.1M MES, 12% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.10 Å R-free 0.223
4LQI Yeast 20S Proteasome in complex with Vibralactone Deposited 2013-07-18 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 9–250（242 aa）
Chain R 9–250（242 aa）
Not recorded 1Y9 vibralactone, bound form × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20 mM magnesium acetate, 100 mM MES, 12% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.227
4LTC Crystal structure of yeast 20S proteasome in complex with enone carmaphycin analogue 6 Deposited 2013-07-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded EC6 N-hexanoyl-L-valyl-N~1~-[(4S,5S,6R)-5-hydroxy-2,6-dimethyloctan-4-yl]-N~5~,N~5~-dimethyl-L-glutamamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.5；297 K；30 mM of magnesium acetate, 100 mM of MES (pH 7.2) and 12% of MPD, vapor diffusion, temperature 297K
Resolution 2.50 Å R-free 0.233
4NNN yCP in complex with MG132 Deposited 2013-11-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 ALD N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S)-1-hydroxy-4-methylpentan-2-yl]-L-leucinamide × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å R-free 0.249
4NNW yCP in complex with Z-Leu-Leu-Leu-ketoaldehyde Deposited 2013-11-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 2MK N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2R,3S)-1,2-dihydroxy-5-methylhexan-3-yl]-L-leucinamide × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 100 mM MES, 13% MPD , pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.60 Å R-free 0.208
4NO1 yCP in complex with Z-Leu-Leu-Leu-B(OH)2 Deposited 2013-11-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CIX N-[(benzyloxy)carbonyl]-L-leucyl-N-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-L-leucinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 100 mM MES, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å R-free 0.214
4NO6 yCP in complex with Z-Leu-Leu-Leu-vinylsulfone Deposited 2013-11-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 2M1 N-[(benzyloxy)carbonyl]-L-leucyl-N-[(3S)-5-methyl-1-(methylsulfonyl)hexan-3-yl]-L-leucinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 100 mM MES, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.00 Å R-free 0.207
4NO8 yCP in complex with Z-Leu-Leu-Leu-ketoamide Deposited 2013-11-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 2LV N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S,3S)-2-hydroxy-5-methyl-1-oxo-1-(phenylamino)hexan-3-yl]-L-leucinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 100 mM MES, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.217
4NO9 yCP in complex with Z-Leu-Leu-Leu-epoxyketone Deposited 2013-11-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 2L0 N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-leucinamide × 3 2LR N-[(benzyloxy)carbonyl]-L-leucyl-N-{(1R,2S)-1-hydroxy-4-methyl-1-[(2R)-2-methyloxiran-2-yl]pentan-2-yl}-L-leucinamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 100 mM MES, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.90 Å R-free 0.203
4Q1S Yeast 20S proteasome in Complex with Kendomycin Deposited 2014-04-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded 2YD (5R,6R,7S,8R,9R,12S,13E,16S,18S,19R,20aR)-4,7,19-trihydroxy-2,6,8,12,14,16,18-heptamethyl-6,7,8,9,10,11,12,15,16,17,18,19,20,20a-tetradecahydro-1,19:5,9-diepoxybenzo[18]annulen-3(5H)-one × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；20 mM MgAc2, 100 mM MES, 13% MPD, pH 6,8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.60 Å R-free 0.224
4QBY yCP in complex with BOC-ALA-ALA-ALA-CHO Deposited 2014-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain D 1–260（260 aa） Fragment：alpha subunit
Chain R 1–260（260 aa） Fragment：alpha subunit
Mutation：wild type Mutation：wild type MG MAGNESIUM ION × 11 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 100 mM MES, 13% MPD , pH 6.8 , VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.00 Å R-free 0.207
4QLQ yCP in complex with tripeptidic epoxyketone inhibitor 8 Deposited 2014-06-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 38N N-(morpholin-4-ylacetyl)-L-alanyl-N-[(2S,4R)-1-cyclohexyl-5-hydroxy-4-methyl-3-oxopentan-2-yl]-O-methyl-L-tyrosinamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20mM MgAc2, 100mM MES, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.40 Å R-free 0.215
4QLS yCP in complex with tripeptidic epoxyketone inhibitor 11 Deposited 2014-06-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 37Y N-(morpholin-4-ylacetyl)-D-alanyl-N-[(2S,4R)-1-cyclohexyl-5-hydroxy-4-methyl-3-oxopentan-2-yl]-O-methyl-L-tyrosinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20mM MGAC2, 13% MPD, PH 6.8, 100mM MES, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.199
4QLT yCP in complex with tripeptidic epoxyketone inhibitor 2 (PR924) Deposited 2014-06-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 39V N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-L-tryptophanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MGAC2, 13% MPD, PH 6.8, 100 mM MES, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.202
4QLU yCP in complex with tripeptidic epoxyketone inhibitor 9 Deposited 2014-06-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 38X N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-1-cyclohexyl-5-hydroxy-4-methyl-3-oxopentan-2-yl]-L-tryptophanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20mM MGAC2, 13% MPD, 100mM MES, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.248
4QLV yCP in complex with tripeptidic epoxyketone inhibitor 17 Deposited 2014-06-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 39Q N-(morpholin-4-ylacetyl)-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-O-methyl-L-tyrosinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20mM MGAC2, 13% MPD, 100mM MES, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.90 Å R-free 0.209
4QUX yCP beta5-A49T-mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.197
4QUY yCP beta5-A49S-mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.226
4QV0 yCP beta5-A49T-A50V-double mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 6 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.10 Å R-free 0.197
4QV1 yCP beta5-M45A mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.50 Å R-free 0.216
4QV3 yCP beta5-M45V mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.211
4QV4 yCP beta5-M45T mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.70 Å R-free 0.213
4QV5 yCP beta5-M45I mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.70 Å R-free 0.217
4QV6 yCP beta5-A49V mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.231
4QV7 yCP beta5-A50V mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.60 Å R-free 0.214
4QV8 yCP beta5-C52F mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.221
4QV9 yCP beta5-C63F mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.60 Å R-free 0.218
4QVL yCP in complex with bortezomib Deposited 2014-07-15 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.215
4QVM yCP beta5-M45A mutant in complex with bortezomib Deposited 2014-07-15 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.215
4QVN yCP beta5-M45V mutant in complex with bortezomib Deposited 2014-07-15 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.213
4QVP yCP beta5-M45T mutant in complex with bortezomib Deposited 2014-07-15 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.30 Å R-free 0.225
4QVQ yCP beta5-M45I mutant in complex with bortezomib Deposited 2014-07-15 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.60 Å R-free 0.219
4QVV yCP beta5-A49V mutant in complex with bortezomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.235
4QVW yCP beta5-A49S-mutant in complex with bortezomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.209
4QVY yCP beta5-A49T-mutant in complex with bortezomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.51 Å R-free 0.219
4QW0 yCP beta5-A49T-A50V-double mutant in complex with bortezomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.211
4QW1 yCP beta5-A50V mutant in complex with bortezomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.216
4QW3 yCP beta5-C63F mutant in complex with bortezomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 5 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.217
4QW4 yCP in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.216
4QW5 yCP beta5-M45A mutant in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 5 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.201
4QW6 yCP beta5-M45V mutant in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 6 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.206
4QW7 yCP beta5-M45T mutant in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.70 Å R-free 0.226
4QWF yCP beta5-M45I mutant in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.203
4QWG yCP beta5-A49V mutant in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 6 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.60 Å R-free 0.243
4QWI yCP beta5-A49S-mutant in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.60 Å R-free 0.214
4QWJ yCP beta5-A49T-mutant in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.228
4QWK yCP beta5-A49T-A50V-double mutant in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.209
4QWL yCP beta5-A50V mutant in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.60 Å R-free 0.219
4QWR yCP beta5-C52F mutant in complex with carfilzomib Deposited 2014-07-17 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.209
4QWS yCP beta5-C63F mutant in complex with carfilzomib Deposited 2014-07-17 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 MG MAGNESIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.214
4QWU yCP beta5-C52F mutant in complex with bortezomib Deposited 2014-07-17 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.218
4QWX yCP in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-17 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K, pH 6.8
Resolution 2.90 Å R-free 0.212
4QXJ yCP beta5-M45A mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-21 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 6 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.222
4QZ0 yCP beta5-M45V mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 6 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.201
4QZ1 yCP beta5-M45T mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 MG MAGNESIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.216
4QZ2 yCP beta5-M45I mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.70 Å R-free 0.219
4QZ3 yCP beta5-A49V mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.207
4QZ4 yCP beta5-A49S mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.218
4QZ5 yCP beta5-A49T-mutant in complex with ONX 0914 Deposited 2014-07-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.224
4QZ6 yCP beta5-A49T-A50V double mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.204
4QZ7 yCP beta5-A50V mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.213
4QZW yCP beta5-C52F mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-29 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.218
4QZX yCP beta5-C63F mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-29 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.60 Å R-free 0.223
4QZZ yCP in complex with Omuralide Deposited 2014-07-29 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 SLA Omuralide, open form × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.208
4R00 yCP beta5-C52F mutant in complex with Omuralide Deposited 2014-07-29 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 5 SLA Omuralide, open form × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.210
4R02 yCP in complex with BSc4999 (alpha-Keto Phenylamide) Deposited 2014-07-29 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 11 3E5 N-[(benzyloxy)carbonyl]-L-leucyl-N-{(2S,3S)-1-[(2,4-dimethylphenyl)amino]-2-hydroxy-5-methyl-1-oxohexan-3-yl}-L-leucinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.50 Å R-free 0.207
4R17 Ligand-induced aziridine-formation at subunit beta5 of the yeast 20S proteasome Deposited 2014-08-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 11 3K4 (2S,3S)-3-methylaziridine-2-carboxylic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.10 Å R-free 0.198
4R18 Ligand-induced Lys33-Thr1 crosslinking at subunit beta5 of the yeast 20S proteasome Deposited 2014-08-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 11 ABA ALPHA-AMINOBUTYRIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.40 Å R-free 0.195
4RUR Yeast 20S proteasome in complex with the alkaloid indolo-phakellin (4) Deposited 2014-11-21 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 3WE (2E,3aR,14aS)-9-bromo-2-imino-1,2,3,5,6,14a-hexahydro-4H,8H-imidazo[4',5':5,6]pyrrolo[1',2':4,5]pyrazino[1,2-a]indol-8-one × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；297 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K, temperature 297K
Resolution 2.50 Å R-free 0.213
4V7O Proteasome Activator Complex Deposited 2009-12-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain AJ 1–250（250 aa）
Chain AV 1–250（250 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
Resolution 3.00 Å R-free 0.250
4V7O Proteasome Activator Complex Deposited 2009-12-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain BE 1–250（250 aa）
Chain BS 1–250（250 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
Resolution 3.00 Å R-free 0.250
4X6Z Yeast 20S proteasome in complex with PR-VI modulator Deposited 2014-12-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain E 1–260（260 aa）
Chain S 1–260（260 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 9 GOL GLYCEROL × 38 MG MAGNESIUM ION × 56 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；30mM magnesium acetate, 12% MPD, 0.1M MES pH 7.2
Resolution 2.70 Å R-free 0.222
4Y69 Yeast 20S proteasome in complex with Ac-PAD-ep Deposited 2015-02-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.217
4Y6A Yeast 20S proteasome beta2-H114D mutant in complex with Ac-PAD-ep Deposited 2015-02-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.60 Å R-free 0.232
4Y6V Yeast 20S proteasome in complex with Ac-PAE-ep Deposited 2015-02-13 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.209
4Y6Z Yeast 20S proteasome in complex with Ac-PAL-ep Deposited 2015-02-13 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.216
4Y70 Yeast 20S proteasome in complex with Ac-LAV-ep Deposited 2015-02-13 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.40 Å R-free 0.226
4Y74 Yeast 20S proteasome in complex with Ac-LAL-ep Deposited 2015-02-13 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.214
4Y75 Yeast 20S proteasome in complex with Ac-PAF-ep Deposited 2015-02-13 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.215
4Y77 Yeast 20S proteasome in complex with Ac-LAF-ep Deposited 2015-02-13 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.215
4Y78 Yeast 20S proteasome in complex with Ac-LAD-ep Deposited 2015-02-13 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.219
4Y7W Yeast 20S proteasome in complex with Ac-LAE-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.217
4Y7X Yeast 20S proteasome in complex with Ac-PAA-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.225
4Y7Y Yeast 20S proteasome in complex with Ac-LAA-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.40 Å R-free 0.235
4Y80 Yeast 20S proteasome in complex with Ac-LAI-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.219
4Y81 Yeast 20S proteasome in complex with Ac-PAY-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.234
4Y82 Yeast 20S proteasome in complex with Ac-LAY-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.207
4Y84 Yeast 20S proteasome in complex with N3-A(4,4-F2P)nLL-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.203
4Y8G Yeast 20S proteasome in complex with N3-APnLL-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.208
4Y8H Yeast 20S proteasome in complex with N3-APAL-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.214
4Y8I Yeast 20S proteasome in complex with Ac-PLL-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.220
4Y8J Yeast 20S proteasome in complex with Ac-LLL-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.224
4Y8K Yeast 20S proteasome in complex with H-APLL-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.236
4Y8L Yeast 20S proteasome in complex with Ac-APLL-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.40 Å R-free 0.224
4Y8M Yeast 20S proteasome beta7-delta7_Cter mutant Deposited 2015-02-16 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.218
4Y8N Yeast 20S proteasome beta7-delta7_Cter mutant in complex with Ac-PAE-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.215
4Y8O Yeast 20S proteasome beta7-delta7_Cter mutant in complex with Ac-PAF-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.231
4Y8P Yeast 20S proteasome beta7-delta7_Cter mutant in complex with Ac-PAL-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.220
4Y8Q Yeast 20S proteasome beta7-delta7_Cter mutant in complex with Ac-PAY-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.228
4Y8R Yeast 20S proteasome beta2-H116D mutant Deposited 2015-02-16 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.222
4Y8S Yeast 20S proteasome beta2-H116D mutant in complex with Ac-LAE-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.221
4Y8T Yeast 20S proteasome beta2-H116D mutant in complex with Ac-PAE-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.209
4Y8U Yeast 20S proteasome beta2-H116D mutant in complex with Ac-PAD-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 6 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.214
4Y9Y Yeast 20S proteasome beta2-H116E mutant Deposited 2015-02-17 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.203
4Y9Z Yeast 20S proteasome beta2-H116E mutant in complex with Ac-LAE-ep Deposited 2015-02-17 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.218
4YA0 Yeast 20S proteasome beta2-H116E mutant in complex with Ac-PAE-ep Deposited 2015-02-17 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.216
4YA1 Yeast 20S proteasome beta2-H116N mutant Deposited 2015-02-17 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded CL CHLORIDE ION × 4 MG MAGNESIUM ION × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.218
4YA2 Yeast 20S proteasome beta2-H116N mutant in complex with Ac-LAE-ep Deposited 2015-02-17 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.205
4YA3 Yeast 20S proteasome beta2-H116N mutant in complex with Ac-PAE-ep Deposited 2015-02-17 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.220
4YA4 Yeast 20S proteasome beta2-H114D mutant Deposited 2015-02-17 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.232
4YA5 Yeast 20S proteasome beta2-H114D mutant in complex with Ac-PAE-ep Deposited 2015-02-17 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.242
4YA7 Yeast 20S proteasome beta2-H114D mutant in complex with Ac-LAE-ep Deposited 2015-02-17 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.237
4YA9 Yeast 20S proteasome beta2-H114D mutant in complex with Ac-LAD-ep Deposited 2015-02-17 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 CL CHLORIDE ION × 1 K POTASSIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.241
4Z1L Yeast 20S proteasome in complex with belactosin C derivative 3 Deposited 2015-03-27 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 4KF (2S,3S)-2-{(1R)-2-[(3,5-dimethoxybenzyl)amino]-1-hydroxy-2-oxoethyl}-3-methylpentanoic acid × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.229
5A5B Structure of the 26S proteasome-Ubp6 complex Deposited 2015-06-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain E 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- HOMEMADE PLUNGER,
Resolution 9.50 Å
5AHJ Yeast 20S proteasome in complex with Macyranone A Deposited 2015-02-06 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 7IM N-[(2S)-3-{[(1S)-1-carboxy-2-phenylethyl]amino}-2-methyl-3-oxopropanoyl]-L-threonyl-N-[(3S,4S)-1,3-dihydroxy-6-methylheptan-4-yl]-L-allothreoninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.8；100 MM MES, PH 7.0 12% MPD 20 MM MGAC2
Resolution 2.80 Å R-free 0.219
5BOU Yeast 20S proteasome in complex with a beta1 / beta2 specific non-peptidic sulfonamide Ligand Deposited 2015-05-27 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 4UC N-[4-(acetylsulfamoyl)phenyl]-2-(4-ethoxyphenyl)quinoline-4-carboxamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.214
5BXL Yeast 20S proteasome beta2-G170A mutant Deposited 2015-06-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.214
5BXN Yeast 20S proteasome beta2-G170A mutant in complex with Bortezomib Deposited 2015-06-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.218
5CGF Yeast 20S proteasome beta5-G48C mutant Deposited 2015-07-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.201
5CGG Yeast 20S proteasome beta5-G48C mutant in complex with alpha-chloroacetamide 1 Deposited 2015-07-09 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.231
5CGH Yeast 20S proteasome beta5-G48C mutant in complex with alpha-chloroacetamide 5 Deposited 2015-07-09 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 54L pent-4-ynoic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.227
5CGI Yeast 20S proteasome beta5-G48C mutant in complex with ONX 0914 Deposited 2015-07-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.206
5CZ4 Yeast 20S proteasome at 2.3 A resolution Deposited 2015-07-31 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.30 Å R-free 0.228
5CZ5 Yeast 20S proteasome beta1-T1A mutant in complex with Carfilzomib Deposited 2015-07-31 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.222
5CZ6 Yeast 20S proteasome beta5-T1A mutant in complex with Syringolin A, propeptide expressed in trans Deposited 2015-07-31 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 SRG (2S)-2-[[(2S)-1-[[(5S,8S,9E)-2,7-dioxo-5-propan-2-yl-1,6-diazacyclododeca-3,9-dien-8-yl]amino]-3-methyl-1-oxo-butan-2-yl]carbamoylamino]-3-methyl-butanoic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.226
5CZ7 Yeast 20S proteasome beta5-T1A beta5-K81R double mutant in complex with Bortezomib, propeptide expressed in cis Deposited 2015-07-31 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 11 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.215
5CZ8 Yeast 20S proteasome beta5-L(-49)S-K33A mutant in complex with Carfilzomib Deposited 2015-07-31 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.215
5CZ9 Yeast 20S proteasome beta5-D17N mutant in complex with Carfilzomib; Propeptide expressed in trans Deposited 2015-07-31 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.223
5CZA Yeast 20S proteasome beta5-D166N mutant Deposited 2015-07-31 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.224
5D0S Yeast 20S proteasome beta5-D166N mutant in complex with Carfilzomib Deposited 2015-08-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.237
5D0T Yeast 20S proteasome beta5-D166N mutant in complex with MG132 Deposited 2015-08-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 11 CL CHLORIDE ION × 4 ALD N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S)-1-hydroxy-4-methylpentan-2-yl]-L-leucinamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.218
5D0V Yeast 20S proteasome beta5-T1C mutant in complex with Carfilzomib Deposited 2015-08-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.220
5D0W Yeast 20S proteasome beta5-T1S mutant Deposited 2015-08-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.203
5D0X Yeast 20S proteasome beta5-T1S mutant in complex with Bortezomib Deposited 2015-08-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.224
5D0Z Yeast 20S proteasome beta5-T1S mutant in complex with Carfilzomib Deposited 2015-08-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.207
5DKI Yeast 20S proteasome in complex with alkyne-PI Deposited 2015-09-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 5 5BZ [(1~{R})-1-[[(2~{S})-2-(hex-5-ynoylamino)-3-phenyl-propanoyl]amino]-3-methyl-butyl]boronic acid × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；30 mM MgAc2, 100 mM MES, 10% MPD
Resolution 2.80 Å R-free 0.219
5DKJ Yeast 20S proteasome in complex with octreotide-PI Deposited 2015-09-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 5BY {2-[2-(2-{4-[(1E)-4-{[(2S)-1-{[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]amino}-1-oxo-3-phenylpropan-2-yl]amino}-4-oxobut-1-en-1-yl]-1H-1,2,3-triazol-1-yl}ethoxy)ethoxy]ethoxy}acetic acid × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；30 mM MgAC2, 10% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.210
5FG7 Yeast 20S proteasome beta2-T1A mutant Deposited 2015-12-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.231
5FG9 Yeast 20S proteasome beta2-T(-2)V mutant Deposited 2015-12-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.230
5FGA Yeast 20S proteasome beta5-K33A mutant (propeptide expressed in trans) Deposited 2015-12-20 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.214
5FGD Yeast 20S proteasome beta5-H(-2)L-T1A double mutant in complex with Carfilzomib Deposited 2015-12-20 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 5 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.208
5FGE Yeast 20S proteasome beta5-H(-2)T-T1A double mutant in complex with Carfilzomib Deposited 2015-12-20 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.214
5FGF Yeast 20S proteasome beta5-H(-2)A-T1A-K81R triple mutant in complex with Carfilzomib Deposited 2015-12-20 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.214
5FGG Yeast 20S proteasome beta5-L(-49S)_D17N double mutant in complex with Carfilzomib Deposited 2015-12-20 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.208
5FGH Yeast 20S proteasome beta5-K33A mutant (propeptide expressed in trans) in complex with MG132 Deposited 2015-12-20 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 ALD N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S)-1-hydroxy-4-methylpentan-2-yl]-L-leucinamide × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.206
5FGI Yeast 20S proteasome beta1-T1A beta2-T1A double mutant in complex with Carfilzomib Deposited 2015-12-20 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 6 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.210
5FHS Yeast 20S proteasome beta5-K33A mutant (propeptide expressed in trans) in complex with Carfilzomib Deposited 2015-12-22 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.212
5JHR Yeast 20S proteasome in complex with the peptidic epoxyketone inhibitor 27 Deposited 2016-04-21 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 3 6KF (2S)-2-azido-N-[(2S)-3-(biphenyl-4-yl)-1-{[(2S)-1-{[(2S,3S,4R)-3,5-dihydroxy-4-methylpentan-2-yl]amino}-1-oxo-3-phenylpropan-2-yl]amino}-1-oxopropan-2-yl]-3-phenylpropanamide (non-preferred name) × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.206
5JHS Yeast 20S proteasome in complex with the peptidic epoxyketone inhibitor 15 Deposited 2016-04-21 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 6KG Nalpha-[(2S)-2-{[(2S)-2-azido-3-phenylpropanoyl]amino}-4-cyclohexylbutanoyl]-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.203
5L52 Yeast 20S proteasome in complex with epoxyketone inhibitor 14 Deposited 2016-05-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 6N5 ~{N}-[(2~{S})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；292 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.210
5L54 Yeast 20S proteasome in complex with epoxyketone inhibitor 16 Deposited 2016-05-27 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 79P (2~{S})-3-(1~{H}-indol-3-yl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.206
5L55 Yeast 20S proteasome in complex with epoxyketone inhibitor 18 Deposited 2016-05-27 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 11 CL CHLORIDE ION × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 6NV ~{N}-[(2~{R})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.225
5L5A Yeast 20S proteasome with human beta5i (1-138; R57T) Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.40 Å R-free 0.219
5L5B Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.211
5L5D Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with ONX 0914 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.221
5L5E Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with carfilzomib Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.227
5L5F Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with bortezomib Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.228
5L5H Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with PR-924 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 39V N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-L-tryptophanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.216
5L5I Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 9 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 38X N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-1-cyclohexyl-5-hydroxy-4-methyl-3-oxopentan-2-yl]-L-tryptophanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.210
5L5J Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 14 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 6N5 ~{N}-[(2~{S})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.200
5L5O Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 16 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 79P (2~{S})-3-(1~{H}-indol-3-yl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.214
5L5P Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 17 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 79L (2~{S})-3-(4-methoxyphenyl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.207
5L5Q Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone 18 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 6NV ~{N}-[(2~{R})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.210
5L5R Yeast 20S proteasome with human beta5i (1-138;V31M) and human beta6 (97-111; 118-133) Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.221
5L5S Yeast 20S proteasome with human beta5i (1-138; V31M) and human beta6 (97-111; 118-133) in complex with PR-924 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 39V N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-L-tryptophanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.211
5L5T Yeast 20S proteasome with human beta5i (1-138; V31M) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 16 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 79P (2~{S})-3-(1~{H}-indol-3-yl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.195
5L5U Yeast 20S proteasome with human beta5i (1-138; V31M) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 17 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 11 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.211
5L5V 'Yeast 20S proteasome with human beta5i (1-138; V31M) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 18 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 6NV ~{N}-[(2~{R})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.208
5L5W Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded CL CHLORIDE ION × 4 MG MAGNESIUM ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.212
5L5X Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with ONX 0914 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.215
5L5Y Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with carfilzomib Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.207
5L5Z Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with bortezomib Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.212
5L60 Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with PR-924 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 11 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 39V N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-L-tryptophanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.212
5L61 Yeast 20S proteasome with human beta5c (1-138) and human beta6 (99-132) in complex with epoxyketone inhibitor 14 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 11 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 6N5 ~{N}-[(2~{S})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.205
5L62 Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 16 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 12 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 79P (2~{S})-3-(1~{H}-indol-3-yl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.204
5L63 Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 17 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 11 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.212
5L64 Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 18 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 6NV ~{N}-[(2~{R})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.223
5L65 Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with carfilzomib Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.201
5L66 Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with bortezomib Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.210
5L67 Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with PR-924 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 39V N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-L-tryptophanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.213
5L68 Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 14 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 6N5 ~{N}-[(2~{S})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.223
5L69 Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 16 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 3 79P (2~{S})-3-(1~{H}-indol-3-yl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.217
5L6A Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 17 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 79L (2~{S})-3-(4-methoxyphenyl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.213
5L6B Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with ONX 0914 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.221
5L6C Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 18 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 6NV ~{N}-[(2~{R})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.217
5LAI Ligand-induced aziridine-formation at the yeast proteasomal subunit beta5 by sulfonate esters Deposited 2016-06-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 11 3K4 (2S,3S)-3-methylaziridine-2-carboxylic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 13% MPD
Resolution 2.50 Å R-free 0.219
5LAJ Ligand-induced Lys33-Thr1 crosslinking at the yeast proteasomal subunit beta5 by sulfonate esters Deposited 2016-06-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 11 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MGAC2, 13% MPD
Resolution 2.90 Å R-free 0.201
5LTT Yeast 20S proteasome with human beta5i (1-138; R57T)in complex with PR-924 Deposited 2016-09-07 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 39V N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-L-tryptophanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.247
5M2B Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with thiazole based inhibitor Ro19 Deposited 2016-10-12 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 7DX (2~{S})-2-cyclohexyl-4-oxidanylidene-4-[[7-(4-phenyl-1,3-thiazol-2-yl)quinolin-2-yl]amino]butanoic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.218
5MP9 26S proteasome in presence of ATP (s1) Deposited 2016-12-16 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain E 1–260（260 aa）
Chain e 1–260（260 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.10 Å
5MPA 26S proteasome in presence of ATP (s2) Deposited 2016-12-16 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain E 1–260（260 aa）
Chain e 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
5MPB 26S proteasome in presence of AMP-PNP (s3) Deposited 2016-12-16 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain E 1–260（260 aa）
Chain e 1–260（260 aa）
Not recorded ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 7.80 Å
5MPC 26S proteasome in presence of BeFx (s4) Deposited 2016-12-16 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric
Chain E 1–260（260 aa）
Chain e 1–260（260 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 7.70 Å
5NIF Yeast 20S proteasome in complex with Blm-pep activator Deposited 2017-03-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain E 1–260（260 aa）
Chain S 1–260（260 aa）
Not recorded CL CHLORIDE ION × 12 MG MAGNESIUM ION × 8 EDO 1,2-ETHANEDIOL × 16 GOL GLYCEROL × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1M MES pH 6.5, 35mM magnesium acetate, 13% MPD
Resolution 3.00 Å R-free 0.227
5WVI The resting state of yeast proteasome Deposited 2016-12-25 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain E 1–260（260 aa）
Chain m 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
5WVK Yeast proteasome-ADP-AlFx Deposited 2016-12-25 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain E 1–260（260 aa）
Chain m 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
6EF0 Yeast 26S proteasome bound to ubiquitinated substrate (1D* motor state) Deposited 2018-08-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain E 1–249（249 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE；specimens were manually blotted with Whatman #1 filter paper
Resolution 4.43 Å
6EF1 Yeast 26S proteasome bound to ubiquitinated substrate (5D motor state) Deposited 2018-08-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain E 9–250（242 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE；specimens were manually blotted with Whatman #1 filter paper
Resolution 4.73 Å
6EF2 Yeast 26S proteasome bound to ubiquitinated substrate (5T motor state) Deposited 2018-08-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain E 2–248（247 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE；specimens were manually blotted with Whatman #1 filter paper
Resolution 4.27 Å
6EF3 Yeast 26S proteasome bound to ubiquitinated substrate (4D motor state) Deposited 2018-08-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain E 1–260（260 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE；specimens were manually blotted with Whatman #1 filter paper
Resolution 4.17 Å
6FVT 26S proteasome, s1 state Deposited 2018-03-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain E 7–250（244 aa）
Chain e 7–250（244 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.10 Å
6FVU 26S proteasome, s2 state Deposited 2018-03-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain E 2–250（249 aa）
Chain e 2–250（249 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
6FVV 26S proteasome, s3 state Deposited 2018-03-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain E 7–250（244 aa）
Chain e 7–250（244 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 2 MG MAGNESIUM ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.40 Å
6FVW 26S proteasome, s4 state Deposited 2018-03-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain E 6–250（245 aa）
Chain e 6–250（245 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
6FVX 26S proteasome, s5 state Deposited 2018-03-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain E 7–250（244 aa）
Chain e 7–250（244 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.90 Å
6FVY 26S proteasome, s6 state Deposited 2018-03-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain E 6–250（245 aa）
Chain e 6–250（245 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.10 Å
6G7F Yeast 20S proteasome in complex with Cystargolide B Deposited 2018-04-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 EPW Cystargolide B- bound form × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8
Resolution 2.70 Å R-free 0.214
6G8M Yeast 20S proteasome in complex with Cystargolide B Derivative 1 Deposited 2018-04-09 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 EQE (2~{S},3~{R})-4-[[(2~{S})-3-methyl-1-[[(2~{S})-3-methyl-1-oxidanylidene-1-phenylmethoxy-butan-2-yl]amino]-1-oxidanylidene-butan-2-yl]amino]-3-oxidanyl-4-oxidanylidene-2-propan-2-yl-butanoic acid × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8
Resolution 2.70 Å R-free 0.214
6G8N Yeast 20S proteasome in complex with Cystargolide B Derivative 2 Deposited 2018-04-09 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 EQB (2~{S},3~{S})-3-methyl-2-[(1~{R})-2-[[(2~{S})-3-methyl-1-[[(2~{S})-3-methyl-1-oxidanylidene-1-phenylmethoxy-butan-2-yl] amino]-1-oxidanylidene-butan-2-yl]amino]-1-oxidanyl-2-oxidanylidene-ethyl]pentanoic acid × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8
Resolution 3.00 Å R-free 0.238
6GOP Yeast 20S Proteasome in complex with Homosalinosporamide A Deposited 2018-06-01 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 F6K Homosalinosporamide A - bound form × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.233
6H39 Yeast 20S proteasome in complex with the peptidic non-covalent binding inhibitor RTS-V5 Deposited 2018-07-17 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 FGY N-(2,2-dimethylpropyl)-N~2~-[4-(hydroxycarbamoyl)benzene-1-carbonyl]-L-asparaginyl-N-benzyl-L-alaninamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.217
6HTB Yeast 20S proteasome with human beta2c (S171G) Deposited 2018-10-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.220
6HTC Yeast 20S proteasome with human beta2c (S171G) in complex with ONX 0914 Deposited 2018-10-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 GQK (2~{S})-3-(4-methoxyphenyl)-~{N}-[(2~{S},3~{R})-4-methyl-3,4-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.218
6HTD Yeast 20S proteasome with human beta2c (S171G) in complex with 4 Deposited 2018-10-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQH (2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.201
6HTP Yeast 20S proteasome with human beta2c (S171G) in complex with 7 Deposited 2018-10-04 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQQ ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]pyrazine-2-carboxamide × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.206
6HTR Yeast 20S proteasome with human beta2c (S171G) in complex with 13 Deposited 2018-10-04 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 GQT (2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.225
6HUB Yeast 20S proteasome with human beta2c (S171G) in complex with 16 Deposited 2018-10-05 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GRW (2~{S})-~{N}-[(2~{S},3~{R})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-butan-2-yl]-2-[[(2~{R})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.212
6HUC Yeast 20S proteasome with human beta2c (S171G) in complex with 18 Deposited 2018-10-05 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GRT (2~{S})-~{N}-[2-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-2-oxidanylidene-ethyl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.218
6HUQ Yeast 20S proteasome with human beta2c (S171G) in complex with 20 Deposited 2018-10-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GT5 ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-methoxy-1-oxidanylidene-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-2-methyl-1,3-thiazole-5-carboxamide × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.207
6HUU Yeast 20S proteasome with human beta2c (S171G) in complex with 29 Deposited 2018-10-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GTW ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-cyclohexyl-1-oxidanylidene-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-2-methyl-1,3-thiazole-5-carboxamide × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.221
6HUV Yeast 20S proteasome with human beta2c (S171G) in complex with 39 Deposited 2018-10-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GT8 (2~{S})-~{N}-[(3~{S},4~{R})-1-cyclohexyl-5-methyl-4,5-bis(oxidanyl)hexan-3-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 6 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.10 Å R-free 0.213
6HV3 Yeast 20S proteasome with human beta2i (1-53) Deposited 2018-10-10 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.209
6HV4 Yeast 20S proteasome with human beta2i (1-53) in complex with ONX 0914 Deposited 2018-10-10 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQK (2~{S})-3-(4-methoxyphenyl)-~{N}-[(2~{S},3~{R})-4-methyl-3,4-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.214
6HV5 Yeast 20S proteasome with human beta2i (1-53) in complex with 4 Deposited 2018-10-10 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQH (2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.204
6HV7 Yeast 20S proteasome with human beta2i (1-53) in complex with 7 Deposited 2018-10-10 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQQ ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]pyrazine-2-carboxamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.40 Å R-free 0.229
6HVA Yeast 20S proteasome with human beta2i (1-53) in complex with 13 Deposited 2018-10-10 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQT (2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.208
6HVR Yeast 20S proteasome with human beta2i (1-53) in complex with 16 Deposited 2018-10-11 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GRW (2~{S})-~{N}-[(2~{S},3~{R})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-butan-2-yl]-2-[[(2~{R})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.219
6HVS Yeast 20S proteasome with human beta2i (1-53) in complex with 18 Deposited 2018-10-11 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GRT (2~{S})-~{N}-[2-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-2-oxidanylidene-ethyl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.10 Å R-free 0.205
6HVT Yeast 20S proteasome with human beta2i (1-53) in complex with 20 Deposited 2018-10-11 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GT5 ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-methoxy-1-oxidanylidene-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-2-methyl-1,3-thiazole-5-carboxamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.206
6HVU Yeast 20S proteasome with human beta2i (1-53) in complex with 29 Deposited 2018-10-11 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GTW ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-cyclohexyl-1-oxidanylidene-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-2-methyl-1,3-thiazole-5-carboxamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.220
6HVV Yeast 20S proteasome with human beta2i (1-53) in complex with 39 Deposited 2018-10-11 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GT8 (2~{S})-~{N}-[(3~{S},4~{R})-1-cyclohexyl-5-methyl-4,5-bis(oxidanyl)hexan-3-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.219
6HVW Yeast 20S proteasome with human beta2i (1-53) in complex with 43 Deposited 2018-10-11 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GVW (2~{S})-~{N}-[(2~{S},3~{R})-1-[(4~{a}~{S},8~{a}~{S})-1,2,3,4,4~{a},5,6,7,8,8~{a}-decahydronaphthalen-2-yl]-4-methyl-3,4-bis(oxidanyl)pentan-2-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.202
6HVX Yeast 20S proteasome in complex with 4 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 GQH (2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.206
6HVY Yeast 20S proteasome in complex with 5 (7- and 6-membered ring) Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GVZ (2~{S})-~{N}-[(2~{S},3~{R})-1-[(1~{S},4~{a}~{S},8~{a}~{R})-1,2,3,4,4~{a},5,6,7,8,8~{a}-decahydronaphthalen-1-yl]-4-methyl-3,4-bis(oxidanyl)pentan-2-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 2 GW2 (2~{S})-~{N}-[(2~{S},3~{S},4~{R})-1-[(1~{S},4~{a}~{S},8~{a}~{R})-1,2,3,4,4~{a},5,6,7,8,8~{a}-decahydronaphthalen-1-yl]-4-methyl-3,5-bis(oxidanyl)pentan-2-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.218
6HW0 Yeast 20S proteasome in complex with 7 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQQ ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]pyrazine-2-carboxamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.206
6HW3 Yeast 20S proteasome in complex with 13 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQT (2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.215
6HW4 Yeast 20S proteasome in complex with 16 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GRW (2~{S})-~{N}-[(2~{S},3~{R})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-butan-2-yl]-2-[[(2~{R})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.210
6HW5 Yeast 20S proteasome in complex with 18 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GRT (2~{S})-~{N}-[2-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-2-oxidanylidene-ethyl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.211
6HW6 Yeast 20S proteasome in complex with 20 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 GT5 ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-methoxy-1-oxidanylidene-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-2-methyl-1,3-thiazole-5-carboxamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.206
6HW7 Yeast 20S proteasome in complex with 29 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 GTW ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-cyclohexyl-1-oxidanylidene-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-2-methyl-1,3-thiazole-5-carboxamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.225
6HW8 Yeast 20S proteasome in complex with 39 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 GT8 (2~{S})-~{N}-[(3~{S},4~{R})-1-cyclohexyl-5-methyl-4,5-bis(oxidanyl)hexan-3-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.206
6HW9 Yeast 20S proteasome in complex with 41b Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 GWK (2~{S})-3-(4-methoxyphenyl)-~{N}-[(2~{S},3~{R})-4-methyl-1-(4-methylcyclohexyl)-3,4-bis(oxidanyl)pentan-2-yl]-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.205
6HWA Yeast 20S proteasome in complex with 43 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 GVW (2~{S})-~{N}-[(2~{S},3~{R})-1-[(4~{a}~{S},8~{a}~{S})-1,2,3,4,4~{a},5,6,7,8,8~{a}-decahydronaphthalen-2-yl]-4-methyl-3,4-bis(oxidanyl)pentan-2-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.216
6HWB Yeast 20S proteasome in complex with 44b Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 GWT (2~{S})-~{N}-[(2~{S},3~{R})-1-(4-cyclohexylcyclohexyl)-4-methyl-3,4-bis(oxidanyl)pentan-2-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ium-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.221
6HWC Yeast 20S proteasome beta2-G45A mutant Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.224
6HWD Yeast 20S proteasome beta2-G45A mutant in complex with bortezomib Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.211
6HWE Yeast 20S proteasome beta2-G45A mutant in complex with carfilzomib Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GWZ (2~{S})-~{N}-[(2~{S})-1-[[(3~{R},4~{S})-2,6-dimethyl-2,3-bis(oxidanyl)heptan-4-yl]amino]-1-oxidanylidene-3-phenyl-propan-2-yl]-4-methyl-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)-4-phenyl-butanoyl]amino]pentanamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.30 Å R-free 0.231
6HWF Yeast 20S proteasome beta2-G45A mutant in complex with ONX 0914 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQK (2~{S})-3-(4-methoxyphenyl)-~{N}-[(2~{S},3~{R})-4-methyl-3,4-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.216
6J2C Yeast proteasome in translocation competent state (C3-a) Deposited 2019-01-01 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain E 1–260（260 aa）
Chain m 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.00 Å
6J2N yeast proteasome in substrate-processing state (C3-b) Deposited 2019-01-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain E 1–260（260 aa）
Chain m 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.50 Å
6J2Q Yeast proteasome in Ub-accepted state (C1-b) Deposited 2019-01-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain E 1–260（260 aa）
Chain m 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6J2X Yeast proteasome in resting state (C1-a) Deposited 2019-01-03 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain E 1–260（260 aa）
Chain m 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6J30 yeast proteasome in Ub-engaged state (C2) Deposited 2019-01-03 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain E 1–260（260 aa）
Chain m 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
6ZOU Yeast 20S proteasome in complex with glidobactin-like natural product HB333 Deposited 2020-07-07 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 QOH 11-methyl-~{N}-[(2~{S},3~{R})-1-[[(5~{S},8~{S},10~{S})-5-methyl-10-oxidanyl-2,7-bis(oxidanylidene)-1,6-diazacyclododec-8-yl]amino]-3-oxidanyl-1-oxidanylidene-butan-2-yl]dodecanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.212
6ZP6 Yeast 20S proteasome in complex with glidobactin-like natural product HB334 Deposited 2020-07-08 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 QOB ~{N}-[(2~{S},3~{R})-1-[[(5~{S},8~{S},10~{S})-5-methyl-10-oxidanyl-2,7-bis(oxidanylidene)-1,6-diazacyclododec-8-yl]amino]-3-oxidanyl-1-oxidanylidene-butan-2-yl]-5-phenyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.202
6ZP8 Yeast 20S proteasome in complex with glidobactin-like natural product HB335 Deposited 2020-07-08 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 QOE (2~{S},3~{R})-~{N}-[(5~{S},8~{S},10~{S})-5-methyl-10-oxidanyl-2,7-bis(oxidanylidene)-1,6-diazacyclododec-8-yl]-3-oxidanyl-2-(3-phenylpropanoylamino)butanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.200
7LS5 Cryo-EM structure of the Pre3-1 20S proteasome core particle Deposited 2021-02-17 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain E 1–260（260 aa）
Chain S 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.74 Å
7LS6 Cryo-EM structure of Pre-15S proteasome core particle assembly intermediate purified from Pre3-1 proteasome mutant (G34D) Deposited 2021-02-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain E 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.17 Å
7LSX Cryo-EM structure of 13S proteasome core particle assembly intermediate purified from Pre3-1 proteasome mutant (G34D) Deposited 2021-02-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain E 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.61 Å
7QO3 Structure of the 26S proteasome-Ubp6 complex in the si state (Core Particle and Lid) Deposited 2021-12-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 41 PDB declaration: 41-meric
Chain E 1–260（260 aa）
Chain e 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.10 Å
7QO5 26S proteasome Rpt1-RK -Ubp6-UbVS complex in the si state Deposited 2021-12-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric
Chain E 1–260（260 aa）
Chain e 1–260（260 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.00 Å
7TEJ Cryo-EM structure of the 20S Alpha 3 Deletion proteasome core particle Deposited 2022-01-05 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain E 1–260（260 aa）
Chain S 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.74 Å
7TEO Cryo-EM structure of the 20S Alpha 3 Deletion proteasome core particle in complex with FUB1 Deposited 2022-01-05 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain E 1–260（260 aa）
Chain S 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.97 Å
8BW1 Yeast 20S proteasome in complex with an engineered fellutamide derivative (C14QAL) Deposited 2022-12-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 3 MYR MYRISTIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；100 mM MES, 20 mM MgAc2, 11% MPD
Resolution 3.25 Å R-free 0.212
8OHZ Yeast 20S proteasome in complex with a photoswitchable cepafungin derivative (transCep1) Deposited 2023-03-21 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 VOT (2~{S},3~{R})-2-[2-[4-[2-(4-ethylphenyl)hydrazinyl]phenyl]ethanoylamino]-~{N}-[(5~{S},8~{S},10~{S})-5-methyl-10-oxidanyl-2,7-bis(oxidanylidene)-1,6-diazacyclododec-8-yl]-3-oxidanyl-butanamide × 4 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.65 Å R-free 0.222
8OI1 Yeast 20S proteasome in complex with a photoswitchable cepafungin derivative (transCep4) Deposited 2023-03-21 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 11 VOX ~{N}-[(2~{S},3~{R})-1-[[(5~{S},8~{S},10~{S})-5-methyl-10-oxidanyl-2,7-bis(oxidanylidene)-1,6-diazacyclododec-8-yl]amino]-3-oxidanyl-1-oxidanylidene-butan-2-yl]-7-[4-[2-(4-methylphenyl)hydrazinyl]phenyl]heptanamide × 4 CL CHLORIDE ION × 2 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.95 Å R-free 0.224
8OLR Structure of yeast 20S proteasome in complex with the natural product beta-lactone inhibitor Cystargolide A Deposited 2023-03-30 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 VSZ Cystargolide A (bound) × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.208
8RHJ Yeast 20S proteasome in complex with a macrocyclic oxindole epoxyketone (compound 5) Deposited 2023-12-15 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.05 Å R-free 0.212
8RHK Yeast 20S proteasome in complex with a linear oxindole epoxyketone (compound 6) Deposited 2023-12-15 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.218
8RHL Yeast 20S proteasome in complex with a linear biarylether epoxyketone (compound 15a) Deposited 2023-12-15 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.20 Å R-free 0.228
8RVL Proteasomal late precursor complex from pre1-1 Deposited 2024-02-01 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain E 1–260（260 aa）
Chain S 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；150 mM NaCl 50 mM Tris-HCl
cryo-EM vitrification conditions Cryogen ETHANE；manual plunge freezing device purchased from 'Neptune Fluid Flow Systems'
Resolution 2.14 Å
8RVO Proteasomal late precursor complex from pre1-1, state 1 Deposited 2024-02-01 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain E 1–260（260 aa）
Chain S 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；150 mM NaCl 50 mM Tris-HCl
cryo-EM vitrification conditions Cryogen ETHANE；manual plunge freezing device purchased from 'Neptune Fluid Flow Systems'
Resolution 2.69 Å
8RVP Proteasomal late precursor complex from pre1-1, state 2 Deposited 2024-02-01 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain E 1–260（260 aa）
Chain S 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；150 mM NaCl 50 mM Tris-HCl
cryo-EM vitrification conditions Cryogen ETHANE；manual plunge freezing device purchased from 'Neptune Fluid Flow Systems'
Resolution 2.28 Å
8RVQ 20S proteasome from pre1-1 Deposited 2024-02-01 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain E 1–260（260 aa）
Chain S 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；150 mM NaCl 50 mM Tris-HCl
cryo-EM vitrification conditions Cryogen ETHANE；manual plunge freezing device purchased from 'Neptune Fluid Flow Systems'
Resolution 2.02 Å
8T08 Preholo-Proteasome from Pre1-1 Pre4-1 Double Mutant Deposited 2023-05-31 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain E 1–260（260 aa）
Chain V 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8T0M Proteasome 20S core particle from Pre1-1 Pre4-1 Double mutant Deposited 2023-06-01 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain E 1–260（260 aa）
Chain S 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
8U6Y Preholo-Proteasome from Beta 3 D205 deletion Deposited 2023-09-14 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain E 1–260（260 aa）
Chain V 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；Fluorinated Fos-Choline was added immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8U7U Proteasome 20S Core Particle from Beta 3 D205 deletion Deposited 2023-09-15 Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain E 1–260（260 aa）
Chain S 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；Fluorinated Fos-Choline was added immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.16 Å
9AW3 Yeast 20S proteasome soaked with MA9 crude extract Deposited 2024-03-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
Resolution 3.42 Å R-free 0.259
9AW5 Yeast 20S proteasome soaked with MA9 fraction E/F Deposited 2024-03-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded SO4 SULFATE ION × 61 MG MAGNESIUM ION × 8 A1A9B (10S,11R,12S,15S,18S)-15-(2-amino-2-oxoethyl)-10,11,23-trihydroxy-18-{[(3R)-3-methyl-2-oxopentanoyl]amino}-9,14,17-trioxo-N-[(1Z)-prop-1-en-1-yl]-8,13,16-triazatetracyclo[18.3.1.0(2,7).0(6,10)]tetracosa-1(24),2,4,6,20,22-hexaene-12-carboxamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
Resolution 3.44 Å R-free 0.252
9AW6 Yeast 20S proteasome soaked with MA9 fraction EF2 Deposited 2024-03-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded SO4 SULFATE ION × 58 A1A9B (10S,11R,12S,15S,18S)-15-(2-amino-2-oxoethyl)-10,11,23-trihydroxy-18-{[(3R)-3-methyl-2-oxopentanoyl]amino}-9,14,17-trioxo-N-[(1Z)-prop-1-en-1-yl]-8,13,16-triazatetracyclo[18.3.1.0(2,7).0(6,10)]tetracosa-1(24),2,4,6,20,22-hexaene-12-carboxamide × 6 MG MAGNESIUM ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
Resolution 3.44 Å R-free 0.235
9C98 Yeast 20S proteasome soaked with isolated TMC-86A Deposited 2024-06-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded SO4 SULFATE ION × 69 MG MAGNESIUM ION × 12 A1AU6 N-[(2S)-3-hydroxy-1-{[(4S)-1-hydroxy-2-(hydroxymethyl)-6-methyl-3-oxoheptan-4-yl]amino}-1-oxopropan-2-yl]butanamide × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；291 K；MES 100 mM pH 6.9, MPD 25% and magnesium acetate 20 mM
Resolution 3.04 Å R-free 0.221
9CGC Yeast 26S proteasome non-substrate-engaged (S1 state) Deposited 2024-06-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain E 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；30 mM HEPES pH7.4, 25 mM NaCl, 25 mM KCl, 3% (v/v) glycerol, 5 mM MgCl2 2 mM ATP and 0.5 mM TCEP, 0.02% NP-40.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.61 Å
9D0T Proteasome core particle assembly intermediate Blm10:13S purified from Saccharomyces cerevisiae Deposited 2024-08-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 12 PDB declaration: dodecameric
Chain E 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.84 Å
9D4C Proteasome core particle assembly intermediate Blm10:alpha-ring purified from Saccharomyces cerevisiae. Deposited 2024-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric
Chain E 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.75 Å
9D4U Core particle assembly intermediate Capless 13S purified from Saccharomyces cerevisiae Deposited 2024-08-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: 11-meric
Chain E 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.55 Å
9EY9 Yeast 20S proteasome in complex with a sybactin derivative (PheSyr) Deposited 2024-04-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 A1H71 sybactin derivative × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.10 Å R-free 0.241
9FRW Yeast 20S proteasome with human beta1i (1-51) Deposited 2024-06-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded CL CHLORIDE ION × 7 MG MAGNESIUM ION × 9 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.85 Å R-free 0.227
9FST Yeast 20S proteasome with human beta1i (1-51) in complex with epoxyketone inhibitor LU-001i Deposited 2024-06-21 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded CL CHLORIDE ION × 4 MG MAGNESIUM ION × 8 A1IF8 azanylidene-[2-[[(2S)-1-[(2S)-2-[[(2S)-1-[[(1S)-2-cyclohexyl-1-[(2R,3S,6R,7S)-3-methanoyl-2,6-dimethyl-6,7-bis(oxidanyl)-1,4-oxazepan-7-yl]ethyl]amino]-1-oxidanylidene-hexan-2-yl]carbamoyl]-4,4-bis(fluoranyl)pyrrolidin-1-yl]-1-oxidanylidene-propan-2-yl]amino]-2-oxidanylidene-ethyl]imino-azanium × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.75 Å R-free 0.245
9FSU Yeast 20S proteasome with human beta1i (1-51) in complex with epoxyketone inhibitor 16 Deposited 2024-06-21 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 SO4 SULFATE ION × 4 A1IFL (2S)-N-[(2S)-1-[[(1S)-2-cyclohexyl-1-[(2R,3S,6R,7S)-3-methanoyl-2,6-dimethyl-6,7-bis(oxidanyl)-1,4-oxazepan-7-yl]ethyl]amino]-3-(4-methoxyphenyl)-1-oxidanylidene-propan-2-yl]-2-(2-morpholin-4-ylethanoylamino)-4-oxidanyl-butanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.75 Å R-free 0.245
9FSV Yeast 20S proteasome with human beta2i (1-53) in complex with epoxyketone inhibitor 16 Deposited 2024-06-22 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 A1IFL (2S)-N-[(2S)-1-[[(1S)-2-cyclohexyl-1-[(2R,3S,6R,7S)-3-methanoyl-2,6-dimethyl-6,7-bis(oxidanyl)-1,4-oxazepan-7-yl]ethyl]amino]-3-(4-methoxyphenyl)-1-oxidanylidene-propan-2-yl]-2-(2-morpholin-4-ylethanoylamino)-4-oxidanyl-butanamide × 4 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.75 Å R-free 0.247
9FT0 Yeast 20S proteasome in complex with epoxyketone inhibitor 16 Deposited 2024-06-23 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 A1IFL (2S)-N-[(2S)-1-[[(1S)-2-cyclohexyl-1-[(2R,3S,6R,7S)-3-methanoyl-2,6-dimethyl-6,7-bis(oxidanyl)-1,4-oxazepan-7-yl]ethyl]amino]-3-(4-methoxyphenyl)-1-oxidanylidene-propan-2-yl]-2-(2-morpholin-4-ylethanoylamino)-4-oxidanyl-butanamide × 4 SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.75 Å R-free 0.232
9FT1 Yeast 20S proteasome in complex with epoxyketone inhibitor 9 Deposited 2024-06-23 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 5 A1IFK tert-butyl 4-[2-[[(2S)-1-[[(2S)-1-[[(1S)-2-cyclohexyl-1-[(2R,3S,6R,7S)-3-methanoyl-2,6-dimethyl-6,7-bis(oxidanyl)-1,4-oxazepan-7-yl]ethyl]amino]-3-(4-methoxyphenyl)-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-3-phenylmethoxy-propan-2-yl]amino]-2-oxidanylidene-ethyl]piperazine-1-carboxylate × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.222
9GBK Blm10-20S proteasome complex from pre1-1 Deposited 2024-07-31 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain E 1–260（260 aa）
Chain S 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；150 mM NaCl 50 mM Tris-HCl
cryo-EM vitrification conditions Cryogen ETHANE；manual plunge freezing device purchased from 'Neptune Fluid Flow Systems'
Resolution 2.39 Å
9IWR 26S proteasome trimer Deposited 2024-07-25 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain E 1–260（260 aa）
Chain e 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；YPD buffer
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 9.10 Å
9QAF Yeast 20S proteasome mutant: beta5_T3M Deposited 2025-02-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.213
9QAI Yeast 20S proteasome mutant: beta5_T3M in complex with MG132 Deposited 2025-02-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 11 CL CHLORIDE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.205
9QB1 Yeast 20S proteasome mutant: beta5_T3M in complex with Bortezomib Deposited 2025-02-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.215
9QB4 Yeast 20S proteasome mutant: beta5_T3M in complex with Carfilzomib Deposited 2025-02-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.207
9QBE Yeast 20S proteasome mutant: beta5_T3M in complex with ONX0914 Deposited 2025-03-02 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.211
9QBI Yeast 20S proteasome mutant: beta5_G128V (b5-propeptide in trans) Deposited 2025-03-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded SO4 SULFATE ION × 2 MG MAGNESIUM ION × 3 CL CHLORIDE ION × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.215
9QBO Yeast 20S proteasome mutant: beta5_G128V (b5-propeptide in trans) in complex with MG132 Deposited 2025-03-03 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded SO4 SULFATE ION × 4 MG MAGNESIUM ION × 2 CL CHLORIDE ION × 4 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.212
9QBW Yeast 20S proteasome mutant: beta5_G128V (b5-propeptide in trans) in complex with Carfilzomib Deposited 2025-03-03 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 3 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 5 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.207
9QBY Yeast 20S proteasome mutant: beta5_G128V (b5-propeptide in trans) in complex with ONX0914 Deposited 2025-03-04 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 3 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.211
9QC9 Yeast 20S proteasome double mutant: beta5_F(-46)S / beta5_G128V Deposited 2025-03-04 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 6 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 8 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.224
9QCF Yeast 20S proteasome mutant: beta1_G128V Deposited 2025-03-04 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.214
9QCK Yeast 20S proteasome mutant: beta1_G128V (b1-propeptide deleted) Deposited 2025-03-04 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 5 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.213
9QD2 Yeast 20S proteasome mutant: beta1_G128V (b1-propeptide deleted) in complex with MG132 Deposited 2025-03-05 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 4 CL CHLORIDE ION × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.214
9QDF Yeast 20S proteasome mutant: beta1_G128V (b1-propeptide deleted) in complex with Bortezomib Deposited 2025-03-06 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 4 CL CHLORIDE ION × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.217
9QDS Yeast 20S proteasome mutant: beta1_G128V (b1-propeptide deleted) in complex with Carfilzomib Deposited 2025-03-06 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 4 CL CHLORIDE ION × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.211
9RL3 13S proteasome precursor complex Deposited 2025-06-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: 13-meric
Chain E 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；150 mM NaCl 50 mM Tris-HCl
cryo-EM vitrification conditions Cryogen ETHANE；manual plunge freezing device purchased from "Neptune FLuid Flow Systems"
Resolution 3.31 Å
9RLA 13S+Beta1 proteasome precursor complex Deposited 2025-06-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: 14-meric
Chain E 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；150 mM NaCl 50 mM Tris-HCl
cryo-EM vitrification conditions Cryogen ETHANE；manual plunge freezing device purchased from "Neptune FLuid Flow Systems"
Resolution 3.16 Å
9RLT dimerised 13S-13S+Beta5 proteasome precursor complexes Deposited 2025-06-17 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 27 PDB declaration: 27-meric
Chain E 1–260（260 aa）
Chain S 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；150 mM NaCl 50 mM Tris-HCl
cryo-EM vitrification conditions Cryogen ETHANE；manual plunge freezing device purchased from "Neptune FLuid Flow Systems"
Resolution 3.09 Å
9RLZ 15S proteasome precursor complex Deposited 2025-06-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: 16-meric
Chain E 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；150 mM NaCl 50 mM Tris-HCl
cryo-EM vitrification conditions Cryogen ETHANE；manual plunge freezing device purchased from "Neptune FLuid Flow Systems"
Resolution 3.12 Å
9RM0 13S+Beta5+Beta6 proteasome precursor complex Deposited 2025-06-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: 15-meric
Chain E 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；150 mM NaCl 50 mM Tris-HCl
cryo-EM vitrification conditions Cryogen ETHANE；manual plunge freezing device purchased from "Neptune FLuid Flow Systems"
Resolution 3.29 Å
9RM1 13S+Beta1+Beta5 proteasome precursor complex Deposited 2025-06-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: 15-meric
Chain E 1–260（260 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；150 mM NaCl 50 mM Tris-HCl
cryo-EM vitrification conditions Cryogen ETHANE；manual plunge freezing device purchased from "Neptune FLuid Flow Systems"
Resolution 4.11 Å
9S78 Yeast 20S Proteasome in Complex with Tellurophene-Tagged Carfilzomib Deposited 2025-08-04 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain D 1–260（260 aa）
Chain R 1–260（260 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.15 Å R-free 0.223

373 other PDB entries and 375 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSA5_YEAST
Isoform —
PDB entities 4
Chains and sequence ranges Author chain D； PDBConstruct 1–260； UniProt 1–260 Author chain R； PDBConstruct 1–260； UniProt 1–260

BJ4_G0043800.mRNA.1.CDS.1

OrganismNot specified

UniProt A0A6A5PTH4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain E； UniProt 1–234 Chain S； UniProt 1–234 Not recorded HLJ1_G0039880.mRNA.1.CDS.1 × 2 （A0A6L1BIF8） BJ4_G0021480.mRNA.1.CDS.1 × 2 （A0A6A5PXC6） HLJ1_G0048980.mRNA.1.CDS.1 × 2 （A0A6A5Q273） 20S proteasome × 2 （P32379） Probable proteasome subunit alpha type-7 × 2 （A0A6A5Q4M4） BJ4_G0020160.mRNA.1.CDS.1 × 2 （A0A6A5PYC9） Proteasome endopeptidase complex × 2 （A0A6A5Q449） Proteasome endopeptidase complex × 2 （A0A6L0YA22） Proteasome subunit beta × 2 （A0A6A5Q0W2） Proteasome subunit beta type-5 × 2 （A0A6V8RU78） Proteasome endopeptidase complex × 2 （A0A6A5Q0P3） Proteasome subunit beta type-7 × 2 （P30657） Proteasome endopeptidase complex × 2 （A0A6L0ZSP2） MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 V08 (2 {R},3 {S})-3-methanoyl-4-methyl-2-hydroxy-pentanoic acid × 6 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES Resolution 3.00 Å R-free 0.216

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7O2L Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3D29 Proteasome Inhibition by Fellutamide B Deposited 2008-05-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain E 2–234（233 aa）
Chain S 2–234（233 aa）
Not recorded HXD (3R)-3-HYDROXYDODECANOIC ACID × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；10 % MPD; 0,1 M MES, 30 mM MgAc2, pH 6,8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.60 Å R-free 0.266
7QO6 26S proteasome Rpt1-RK -Ubp6-UbVS complex in the s2 state Deposited 2021-12-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric
Chain F 1–234（234 aa）
Chain f 1–234（234 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
9AW7 Yeast 20S proteasome soaked with isolated TMC-95B Deposited 2024-03-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain E 1–234（234 aa）
Chain S 1–234（234 aa）
Not recorded SO4 SULFATE ION × 85 MG MAGNESIUM ION × 12 A1AHA TMC-95B × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 ACY ACETIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
Resolution 2.91 Å R-free 0.232
9C97 Yeast 20S proteasome soaked with BRA-346 fraction Deposited 2024-06-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain E 1–234（234 aa）
Chain S 1–234（234 aa）
Not recorded SO4 SULFATE ION × 44 MG MAGNESIUM ION × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；291 K；MES 100 mM pH 6.9, MPD 25% and magnesium acetate 20 mM
Resolution 3.33 Å R-free 0.252

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name A0A6A5PTH4_YEASX
Isoform —
PDB entities 5
Chains and sequence ranges Author chain E； PDBConstruct 1–234； UniProt 1–234 Author chain S； PDBConstruct 1–234； UniProt 1–234

Probable proteasome subunit alpha type-7

OrganismNot specified

UniProt A0A6A5Q4M4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain F； UniProt 1–288 Chain T； UniProt 1–288 Not recorded HLJ1_G0039880.mRNA.1.CDS.1 × 2 （A0A6L1BIF8） BJ4_G0021480.mRNA.1.CDS.1 × 2 （A0A6A5PXC6） HLJ1_G0048980.mRNA.1.CDS.1 × 2 （A0A6A5Q273） 20S proteasome × 2 （P32379） BJ4_G0043800.mRNA.1.CDS.1 × 2 （A0A6A5PTH4） BJ4_G0020160.mRNA.1.CDS.1 × 2 （A0A6A5PYC9） Proteasome endopeptidase complex × 2 （A0A6A5Q449） Proteasome endopeptidase complex × 2 （A0A6L0YA22） Proteasome subunit beta × 2 （A0A6A5Q0W2） Proteasome subunit beta type-5 × 2 （A0A6V8RU78） Proteasome endopeptidase complex × 2 （A0A6A5Q0P3） Proteasome subunit beta type-7 × 2 （P30657） Proteasome endopeptidase complex × 2 （A0A6L0ZSP2） MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 V08 (2 {R},3 {S})-3-methanoyl-4-methyl-2-hydroxy-pentanoic acid × 6 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES Resolution 3.00 Å R-free 0.216

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7O2L Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3D29 Proteasome Inhibition by Fellutamide B Deposited 2008-05-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain F 5–248（244 aa）
Chain T 5–248（244 aa）
Not recorded HXD (3R)-3-HYDROXYDODECANOIC ACID × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；10 % MPD; 0,1 M MES, 30 mM MgAc2, pH 6,8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.60 Å R-free 0.266
7QO6 26S proteasome Rpt1-RK -Ubp6-UbVS complex in the s2 state Deposited 2021-12-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric
Chain G 1–288（288 aa）
Chain g 1–288（288 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
9AW3 Yeast 20S proteasome soaked with MA9 crude extract Deposited 2024-03-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain F 2–288（287 aa）
Chain T 2–288（287 aa）
Not recorded MG MAGNESIUM ION × 7 SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
Resolution 3.42 Å R-free 0.259
9AW7 Yeast 20S proteasome soaked with isolated TMC-95B Deposited 2024-03-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain F 2–288（287 aa）
Chain T 2–288（287 aa）
Not recorded SO4 SULFATE ION × 85 MG MAGNESIUM ION × 12 A1AHA TMC-95B × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 ACY ACETIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
Resolution 2.91 Å R-free 0.232
9C97 Yeast 20S proteasome soaked with BRA-346 fraction Deposited 2024-06-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain F 2–288（287 aa）
Chain T 2–288（287 aa）
Not recorded SO4 SULFATE ION × 44 MG MAGNESIUM ION × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；291 K；MES 100 mM pH 6.9, MPD 25% and magnesium acetate 20 mM
Resolution 3.33 Å R-free 0.252

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name A0A6A5Q4M4_YEASX
Isoform —
PDB entities 6
Chains and sequence ranges Author chain F； PDBConstruct 1–288； UniProt 1–288 Author chain T； PDBConstruct 1–288； UniProt 1–288

BJ4_G0020160.mRNA.1.CDS.1

OrganismNot specified

UniProt A0A6A5PYC9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain G； UniProt 1–252 Chain U； UniProt 1–252 Not recorded HLJ1_G0039880.mRNA.1.CDS.1 × 2 （A0A6L1BIF8） BJ4_G0021480.mRNA.1.CDS.1 × 2 （A0A6A5PXC6） HLJ1_G0048980.mRNA.1.CDS.1 × 2 （A0A6A5Q273） 20S proteasome × 2 （P32379） BJ4_G0043800.mRNA.1.CDS.1 × 2 （A0A6A5PTH4） Probable proteasome subunit alpha type-7 × 2 （A0A6A5Q4M4） Proteasome endopeptidase complex × 2 （A0A6A5Q449） Proteasome endopeptidase complex × 2 （A0A6L0YA22） Proteasome subunit beta × 2 （A0A6A5Q0W2） Proteasome subunit beta type-5 × 2 （A0A6V8RU78） Proteasome endopeptidase complex × 2 （A0A6A5Q0P3） Proteasome subunit beta type-7 × 2 （P30657） Proteasome endopeptidase complex × 2 （A0A6L0ZSP2） MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 V08 (2 {R},3 {S})-3-methanoyl-4-methyl-2-hydroxy-pentanoic acid × 6 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES Resolution 3.00 Å R-free 0.216

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7O2L Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3D29 Proteasome Inhibition by Fellutamide B Deposited 2008-05-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain G 10–252（243 aa）
Chain U 10–252（243 aa）
Not recorded HXD (3R)-3-HYDROXYDODECANOIC ACID × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；10 % MPD; 0,1 M MES, 30 mM MgAc2, pH 6,8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.60 Å R-free 0.266
7QO6 26S proteasome Rpt1-RK -Ubp6-UbVS complex in the s2 state Deposited 2021-12-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric
Chain A 1–252（252 aa）
Chain a 1–252（252 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
9C97 Yeast 20S proteasome soaked with BRA-346 fraction Deposited 2024-06-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain G 1–252（252 aa）
Chain U 1–252（252 aa）
Not recorded SO4 SULFATE ION × 44 MG MAGNESIUM ION × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；291 K；MES 100 mM pH 6.9, MPD 25% and magnesium acetate 20 mM
Resolution 3.33 Å R-free 0.252

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name A0A6A5PYC9_YEASX
Isoform —
PDB entities 7
Chains and sequence ranges Author chain G； PDBConstruct 1–252； UniProt 1–252 Author chain U； PDBConstruct 1–252； UniProt 1–252

Proteasome endopeptidase complex

OrganismNot specified

UniProt A0A6A5Q449

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain H； UniProt 30–261 Chain V； UniProt 30–261 Not recorded HLJ1_G0039880.mRNA.1.CDS.1 × 2 （A0A6L1BIF8） BJ4_G0021480.mRNA.1.CDS.1 × 2 （A0A6A5PXC6） HLJ1_G0048980.mRNA.1.CDS.1 × 2 （A0A6A5Q273） 20S proteasome × 2 （P32379） BJ4_G0043800.mRNA.1.CDS.1 × 2 （A0A6A5PTH4） Probable proteasome subunit alpha type-7 × 2 （A0A6A5Q4M4） BJ4_G0020160.mRNA.1.CDS.1 × 2 （A0A6A5PYC9） Proteasome endopeptidase complex × 2 （A0A6L0YA22） Proteasome subunit beta × 2 （A0A6A5Q0W2） Proteasome subunit beta type-5 × 2 （A0A6V8RU78） Proteasome endopeptidase complex × 2 （A0A6A5Q0P3） Proteasome subunit beta type-7 × 2 （P30657） Proteasome endopeptidase complex × 2 （A0A6L0ZSP2） MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 V08 (2 {R},3 {S})-3-methanoyl-4-methyl-2-hydroxy-pentanoic acid × 6 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES Resolution 3.00 Å R-free 0.216

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7O2L Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3D29 Proteasome Inhibition by Fellutamide B Deposited 2008-05-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain H 30–251（222 aa）
Chain V 30–251（222 aa）
Not recorded HXD (3R)-3-HYDROXYDODECANOIC ACID × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；10 % MPD; 0,1 M MES, 30 mM MgAc2, pH 6,8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.60 Å R-free 0.266
7QO6 26S proteasome Rpt1-RK -Ubp6-UbVS complex in the s2 state Deposited 2021-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric
Chain 2 1–261（261 aa）
Chain i 1–261（261 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
9AW3 Yeast 20S proteasome soaked with MA9 crude extract Deposited 2024-03-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain H 30–261（232 aa）
Chain V 30–261（232 aa）
Not recorded MG MAGNESIUM ION × 7 SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
Resolution 3.42 Å R-free 0.259
9AW7 Yeast 20S proteasome soaked with isolated TMC-95B Deposited 2024-03-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain H 30–261（232 aa）
Chain V 30–261（232 aa）
Not recorded SO4 SULFATE ION × 85 MG MAGNESIUM ION × 12 A1AHA TMC-95B × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 ACY ACETIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
Resolution 2.91 Å R-free 0.232
9C97 Yeast 20S proteasome soaked with BRA-346 fraction Deposited 2024-06-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain H 30–261（232 aa）
Chain V 30–261（232 aa）
Not recorded SO4 SULFATE ION × 44 MG MAGNESIUM ION × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；291 K；MES 100 mM pH 6.9, MPD 25% and magnesium acetate 20 mM
Resolution 3.33 Å R-free 0.252
9EY9 Yeast 20S proteasome in complex with a sybactin derivative (PheSyr) Deposited 2024-04-09 Different construct Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain H 31–261（231 aa）
Chain V 31–261（231 aa）
Not recorded MG MAGNESIUM ION × 8 A1H71 sybactin derivative × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.10 Å R-free 0.241

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name A0A6A5Q449_YEASX
Isoform —
PDB entities 8
Chains and sequence ranges Author chain H； PDBConstruct 1–232； UniProt 30–261 Author chain V； PDBConstruct 1–232； UniProt 30–261

Proteasome endopeptidase complex

OrganismNot specified

UniProt A0A6L0YA22

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain I； UniProt 1–205 Chain W； UniProt 1–205 Not recorded HLJ1_G0039880.mRNA.1.CDS.1 × 2 （A0A6L1BIF8） BJ4_G0021480.mRNA.1.CDS.1 × 2 （A0A6A5PXC6） HLJ1_G0048980.mRNA.1.CDS.1 × 2 （A0A6A5Q273） 20S proteasome × 2 （P32379） BJ4_G0043800.mRNA.1.CDS.1 × 2 （A0A6A5PTH4） Probable proteasome subunit alpha type-7 × 2 （A0A6A5Q4M4） BJ4_G0020160.mRNA.1.CDS.1 × 2 （A0A6A5PYC9） Proteasome endopeptidase complex × 2 （A0A6A5Q449） Proteasome subunit beta × 2 （A0A6A5Q0W2） Proteasome subunit beta type-5 × 2 （A0A6V8RU78） Proteasome endopeptidase complex × 2 （A0A6A5Q0P3） Proteasome subunit beta type-7 × 2 （P30657） Proteasome endopeptidase complex × 2 （A0A6L0ZSP2） MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 V08 (2 {R},3 {S})-3-methanoyl-4-methyl-2-hydroxy-pentanoic acid × 6 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES Resolution 3.00 Å R-free 0.216

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7O2L Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3D29 Proteasome Inhibition by Fellutamide B Deposited 2008-05-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain I 2–205（204 aa）
Chain W 2–205（204 aa）
Not recorded HXD (3R)-3-HYDROXYDODECANOIC ACID × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；10 % MPD; 0,1 M MES, 30 mM MgAc2, pH 6,8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.60 Å R-free 0.266
7QO6 26S proteasome Rpt1-RK -Ubp6-UbVS complex in the s2 state Deposited 2021-12-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric
Chain 3 1–205（205 aa）
Chain j 1–205（205 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
9AW7 Yeast 20S proteasome soaked with isolated TMC-95B Deposited 2024-03-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain I 1–205（205 aa）
Chain W 1–205（205 aa）
Not recorded SO4 SULFATE ION × 85 MG MAGNESIUM ION × 12 A1AHA TMC-95B × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 ACY ACETIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
Resolution 2.91 Å R-free 0.232
9C97 Yeast 20S proteasome soaked with BRA-346 fraction Deposited 2024-06-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain I 1–205（205 aa）
Chain W 1–205（205 aa）
Not recorded SO4 SULFATE ION × 44 MG MAGNESIUM ION × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；291 K；MES 100 mM pH 6.9, MPD 25% and magnesium acetate 20 mM
Resolution 3.33 Å R-free 0.252

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name A0A6L0YA22_YEASX
Isoform —
PDB entities 9
Chains and sequence ranges Author chain I； PDBConstruct 1–205； UniProt 1–205 Author chain W； PDBConstruct 1–205； UniProt 1–205

Proteasome subunit beta

OrganismNot specified

UniProt A0A6A5Q0W2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain J； UniProt 1–198 Chain X； UniProt 1–198 Not recorded HLJ1_G0039880.mRNA.1.CDS.1 × 2 （A0A6L1BIF8） BJ4_G0021480.mRNA.1.CDS.1 × 2 （A0A6A5PXC6） HLJ1_G0048980.mRNA.1.CDS.1 × 2 （A0A6A5Q273） 20S proteasome × 2 （P32379） BJ4_G0043800.mRNA.1.CDS.1 × 2 （A0A6A5PTH4） Probable proteasome subunit alpha type-7 × 2 （A0A6A5Q4M4） BJ4_G0020160.mRNA.1.CDS.1 × 2 （A0A6A5PYC9） Proteasome endopeptidase complex × 2 （A0A6A5Q449） Proteasome endopeptidase complex × 2 （A0A6L0YA22） Proteasome subunit beta type-5 × 2 （A0A6V8RU78） Proteasome endopeptidase complex × 2 （A0A6A5Q0P3） Proteasome subunit beta type-7 × 2 （P30657） Proteasome endopeptidase complex × 2 （A0A6L0ZSP2） MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 V08 (2 {R},3 {S})-3-methanoyl-4-methyl-2-hydroxy-pentanoic acid × 6 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES Resolution 3.00 Å R-free 0.216

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7O2L Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3D29 Proteasome Inhibition by Fellutamide B Deposited 2008-05-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain J 1–198（198 aa）
Chain X 1–198（198 aa）
Not recorded HXD (3R)-3-HYDROXYDODECANOIC ACID × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；10 % MPD; 0,1 M MES, 30 mM MgAc2, pH 6,8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.60 Å R-free 0.266
7QO6 26S proteasome Rpt1-RK -Ubp6-UbVS complex in the s2 state Deposited 2021-12-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric
Chain 4 1–198（198 aa）
Chain k 1–198（198 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
9AW7 Yeast 20S proteasome soaked with isolated TMC-95B Deposited 2024-03-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain J 1–198（198 aa）
Chain X 1–198（198 aa）
Not recorded SO4 SULFATE ION × 85 MG MAGNESIUM ION × 12 A1AHA TMC-95B × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 ACY ACETIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
Resolution 2.91 Å R-free 0.232
9C97 Yeast 20S proteasome soaked with BRA-346 fraction Deposited 2024-06-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain J 1–198（198 aa）
Chain X 1–198（198 aa）
Not recorded SO4 SULFATE ION × 44 MG MAGNESIUM ION × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；291 K；MES 100 mM pH 6.9, MPD 25% and magnesium acetate 20 mM
Resolution 3.33 Å R-free 0.252

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name A0A6A5Q0W2_YEASX
Isoform —
PDB entities 10
Chains and sequence ranges Author chain J； PDBConstruct 1–198； UniProt 1–198 Author chain X； PDBConstruct 1–198； UniProt 1–198

Proteasome subunit beta type-5

OrganismNot specified

UniProt A0A6V8RU78

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain K； UniProt 76–287 Chain Y； UniProt 76–287 Not recorded HLJ1_G0039880.mRNA.1.CDS.1 × 2 （A0A6L1BIF8） BJ4_G0021480.mRNA.1.CDS.1 × 2 （A0A6A5PXC6） HLJ1_G0048980.mRNA.1.CDS.1 × 2 （A0A6A5Q273） 20S proteasome × 2 （P32379） BJ4_G0043800.mRNA.1.CDS.1 × 2 （A0A6A5PTH4） Probable proteasome subunit alpha type-7 × 2 （A0A6A5Q4M4） BJ4_G0020160.mRNA.1.CDS.1 × 2 （A0A6A5PYC9） Proteasome endopeptidase complex × 2 （A0A6A5Q449） Proteasome endopeptidase complex × 2 （A0A6L0YA22） Proteasome subunit beta × 2 （A0A6A5Q0W2） Proteasome endopeptidase complex × 2 （A0A6A5Q0P3） Proteasome subunit beta type-7 × 2 （P30657） Proteasome endopeptidase complex × 2 （A0A6L0ZSP2） MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 V08 (2 {R},3 {S})-3-methanoyl-4-methyl-2-hydroxy-pentanoic acid × 6 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES Resolution 3.00 Å R-free 0.216

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence
UniProt name A0A6V8RU78_YEASX
Isoform —
PDB entities 11
Chains and sequence ranges Author chain K； PDBConstruct 1–212； UniProt 76–287 Author chain Y； PDBConstruct 1–212； UniProt 76–287

Proteasome endopeptidase complex

OrganismNot specified

UniProt A0A6A5Q0P3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain L； UniProt 20–241 Chain Z； UniProt 20–241 Not recorded HLJ1_G0039880.mRNA.1.CDS.1 × 2 （A0A6L1BIF8） BJ4_G0021480.mRNA.1.CDS.1 × 2 （A0A6A5PXC6） HLJ1_G0048980.mRNA.1.CDS.1 × 2 （A0A6A5Q273） 20S proteasome × 2 （P32379） BJ4_G0043800.mRNA.1.CDS.1 × 2 （A0A6A5PTH4） Probable proteasome subunit alpha type-7 × 2 （A0A6A5Q4M4） BJ4_G0020160.mRNA.1.CDS.1 × 2 （A0A6A5PYC9） Proteasome endopeptidase complex × 2 （A0A6A5Q449） Proteasome endopeptidase complex × 2 （A0A6L0YA22） Proteasome subunit beta × 2 （A0A6A5Q0W2） Proteasome subunit beta type-5 × 2 （A0A6V8RU78） Proteasome subunit beta type-7 × 2 （P30657） Proteasome endopeptidase complex × 2 （A0A6L0ZSP2） MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 V08 (2 {R},3 {S})-3-methanoyl-4-methyl-2-hydroxy-pentanoic acid × 6 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES Resolution 3.00 Å R-free 0.216

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7O2L Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3D29 Proteasome Inhibition by Fellutamide B Deposited 2008-05-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain L 20–241（222 aa）
Chain Z 20–241（222 aa）
Not recorded HXD (3R)-3-HYDROXYDODECANOIC ACID × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；10 % MPD; 0,1 M MES, 30 mM MgAc2, pH 6,8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.60 Å R-free 0.266
7QO6 26S proteasome Rpt1-RK -Ubp6-UbVS complex in the s2 state Deposited 2021-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric
Chain 6 1–241（241 aa）
Chain m 1–241（241 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
9AW3 Yeast 20S proteasome soaked with MA9 crude extract Deposited 2024-03-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain L 20–241（222 aa）
Chain Z 20–241（222 aa）
Not recorded MG MAGNESIUM ION × 7 SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
Resolution 3.42 Å R-free 0.259
9AW7 Yeast 20S proteasome soaked with isolated TMC-95B Deposited 2024-03-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain L 20–241（222 aa）
Chain Z 20–241（222 aa）
Not recorded SO4 SULFATE ION × 85 MG MAGNESIUM ION × 12 A1AHA TMC-95B × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 ACY ACETIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
Resolution 2.91 Å R-free 0.232
9C97 Yeast 20S proteasome soaked with BRA-346 fraction Deposited 2024-06-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain L 20–241（222 aa）
Chain Z 20–241（222 aa）
Not recorded SO4 SULFATE ION × 44 MG MAGNESIUM ION × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；291 K；MES 100 mM pH 6.9, MPD 25% and magnesium acetate 20 mM
Resolution 3.33 Å R-free 0.252

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name A0A6A5Q0P3_YEASX
Isoform —
PDB entities 12
Chains and sequence ranges Author chain L； PDBConstruct 1–222； UniProt 20–241 Author chain Z； PDBConstruct 1–222； UniProt 20–241

Proteasome subunit beta type-7

OrganismNot specified

UniProt P30657

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain M； UniProt 21–266 Chain a； UniProt 21–266 Not recorded HLJ1_G0039880.mRNA.1.CDS.1 × 2 （A0A6L1BIF8） BJ4_G0021480.mRNA.1.CDS.1 × 2 （A0A6A5PXC6） HLJ1_G0048980.mRNA.1.CDS.1 × 2 （A0A6A5Q273） 20S proteasome × 2 （P32379） BJ4_G0043800.mRNA.1.CDS.1 × 2 （A0A6A5PTH4） Probable proteasome subunit alpha type-7 × 2 （A0A6A5Q4M4） BJ4_G0020160.mRNA.1.CDS.1 × 2 （A0A6A5PYC9） Proteasome endopeptidase complex × 2 （A0A6A5Q449） Proteasome endopeptidase complex × 2 （A0A6L0YA22） Proteasome subunit beta × 2 （A0A6A5Q0W2） Proteasome subunit beta type-5 × 2 （A0A6V8RU78） Proteasome endopeptidase complex × 2 （A0A6A5Q0P3） Proteasome endopeptidase complex × 2 （A0A6L0ZSP2） MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 V08 (2 {R},3 {S})-3-methanoyl-4-methyl-2-hydroxy-pentanoic acid × 6 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES Resolution 3.00 Å R-free 0.216

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7O2L Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1FNT CRYSTAL STRUCTURE OF THE 20S PROTEASOME FROM YEAST IN COMPLEX WITH THE PROTEASOME ACTIVATOR PA26 FROM TRYPANOSOME BRUCEI AT 3.2 ANGSTROMS RESOLUTION Deposited 2000-08-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain N 67–299（233 aa）
Chain b 67–299（233 aa）
Not recorded MG MAGNESIUM ION × 14 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；0.1 M NaHEPES, 40% MPD, 0.2 M NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP
Resolution 3.20 Å R-free 0.325
1G0U A GATED CHANNEL INTO THE PROTEASOME CORE PARTICLE Deposited 2000-10-09 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 1–266（266 aa）
Chain M 1–266（266 aa）
Not recorded MG MAGNESIUM ION × 20 X-RAY DIFFRACTION
X-ray crystallization conditions hanging drop vapour-diffusion；pH 6.7；297 K；22mM Magnesium acetate, 4% Dimethylsulfoxide, 100mM morpholinoethanesulfonic acid, 11% methylpentanediol, pH 6.7, hanging drop vapour-diffusion, temperature 297K
Resolution 2.40 Å R-free 0.303
1G65 Crystal structure of epoxomicin:20s proteasome reveals a molecular basis for selectivity of alpha,beta-epoxyketone proteasome inhibitors Deposited 2000-11-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain 1 34–266（233 aa）
Chain M 34–266（233 aa）
Not recorded MG MAGNESIUM ION × 10 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；0.1M Mes, pH 6.8 11% MPD 25mM MgAc2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
Resolution 2.25 Å R-free 0.336
1JD2 Crystal Structure of the yeast 20S Proteasome:TMC-95A complex: A non-covalent Proteasome Inhibitor Deposited 2001-06-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain M 34–266（233 aa）
Chain T 34–266（233 aa）
Not recorded MG MAGNESIUM ION × 10 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；MPD, MES, DMSO, magnesium Acetate, pH 6.4, VAPOR DIFFUSION, HANGING DROP at 293K
Resolution 3.00 Å R-free 0.336
1RYP CRYSTAL STRUCTURE OF THE 20S PROTEASOME FROM YEAST AT 2.4 ANGSTROMS RESOLUTION Deposited 1997-02-26 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 2 34–266（233 aa）
Chain N 34–266（233 aa）
Mutation：CHAINS H, V, T1A, CHAIN L, Z, K33R Mutation：CHAINS H, V, T1A, CHAIN L, Z, K33R MG MAGNESIUM ION × 20 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.9；pH 6.9
Resolution 1.90 Å R-free 0.330
1Z7Q Crystal structure of the 20s proteasome from yeast in complex with the proteasome activator PA26 from Trypanosome brucei at 3.2 angstroms resolution Deposited 2005-03-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric
Chain N 34–266（233 aa）
Chain b 34–266（233 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.1 M NaHEPES, 40% MPD, 0.2 M NACL, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 3.22 Å R-free 0.308
2F16 Crystal structure of the yeast 20S proteasome in complex with bortezomib Deposited 2005-11-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa）
Chain M 34–266（233 aa）
Not recorded BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；12 % MPD 28mM MgAc2 50mM MES-NaOH, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.264
2FAK Crystal structure of Salinosporamide A in complex with the yeast 20S proteasome Deposited 2005-12-07 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa）
Chain M 34–266（233 aa）
Not recorded SA1 (3AR,6R,6AS)-6-((S)-((S)-CYCLOHEX-2-ENYL)(HYDROXY)METHYL)-6A-METHYL-4-OXO-HEXAHYDRO-2H-FURO[3,2-C]PYRROLE-6-CARBALDEHYDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；Crystals appeared after one week; Crystallization conditions: 40mg/ml yeast 20S proteasome 0.1M MES; 28mM MgAc2, 12% MPD 6h crystal soak with 10mM SalA, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.254
2GPL TMC-95 based biphenyl-ether macrocycles: specific proteasome inhibitors Deposited 2006-04-18 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa）
Chain M 34–266（233 aa）
Not recorded BIQ BENZYL [12-(2-AMINO-2-OXOETHYL)-4-NITRO-10,13-DIOXO-15-[(PROPYLAMINO)CARBONYL]-2-OXA-11,14-DIAZATRICYCLO[15 .2.2.1~3,7~]DOCOSA-1(19),3(22),4,6,17,20-HEXAEN-9-YL]CARBAMATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；20mM MgAc2; 0.1M MES, 12% MPD; crystals appeared within 7 days; crystal size 0.2 x 0.2 x 0.6 mm3, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.81 Å R-free 0.241
2ZCY yeast 20S proteasome:syringolin A-complex Deposited 2007-11-15 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 0 1–266（266 aa）
Chain M 1–266（266 aa）
Not recorded SRG (2S)-2-[[(2S)-1-[[(5S,8S,9E)-2,7-dioxo-5-propan-2-yl-1,6-diazacyclododeca-3,9-dien-8-yl]amino]-3-methyl-1-oxo-butan-2-yl]carbamoylamino]-3-methyl-butanoic acid × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；294 K；0.1M MES, 12% MPD, 30mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 294K
Resolution 2.90 Å R-free 0.245
3BDM yeast 20S proteasome:glidobactin A-complex Deposited 2007-11-15 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 0 1–266（266 aa）
Chain M 1–266（266 aa）
Not recorded GDT (2E,4E)-N-[(2S,3R)-3-hydroxy-1-[[(3Z,5S,8S,10S)-10-hydroxy-5-methyl-2,7-dioxo-1,6-diazacyclododec-3-en-8-yl]amino]-1-ox obutan-2-yl]dodeca-2,4-dienamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；294 K；100mM Mes, 14% MPD, 30mM MgAc2, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K
Resolution 2.70 Å R-free 0.262
3DY3 Crystal structure of yeast 20S proteasome in complex with the epimer form of spirolactacystin Deposited 2008-07-25 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa） Fragment：UNP residues 34-266
Chain M 34–266（233 aa） Fragment：UNP residues 34-266
Not recorded SLR (3R,4R)-3-hydroxy-2-[(1S)-1-hydroxy-2-methylpropyl]-4-methyl-5-oxo-D-proline × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；0.1 M MES, 10 % MPD, 20 mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.81 Å R-free 0.245
3DY4 Crystal structure of yeast 20S proteasome in complex with spirolactacystin Deposited 2008-07-25 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa） Fragment：UNP residues 34-266
Chain M 34–266（233 aa） Fragment：UNP residues 34-266
Not recorded SLA Omuralide, open form × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；0.1 M MES, 10 % MPD, 20 mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.80 Å R-free 0.256
3E47 Crystal Structure of the Yeast 20S Proteasome in Complex with Homobelactosin C Deposited 2008-08-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa）
Chain M 34–266（233 aa）
Not recorded ESY benzyl N-[(benzyloxy)carbonyl]-L-alanyl-N~6~-[(2R,3S,4S)-3-formyl-2-hydroxy-4-methylhexanoyl]-L-lysinate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；20% MPD, 0.1M MES, 30mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 3.00 Å R-free 0.245
3GPJ Crystal structure of the yeast 20S proteasome in complex with syringolin B Deposited 2009-03-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa） Fragment：sequence database residues 34-266
Chain M 34–266（233 aa） Fragment：sequence database residues 34-266
Not recorded SY2 N-{[(1S)-2-methyl-1-{[(5S,8S)-5-(1-methylethyl)-2,7-dioxo-1,6-diazacyclododec-3-en-8-yl]carbamoyl}propyl]carbamoyl}-L-valine × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；12% MPD, 0.1M MES, pH 6.7, 20mM MgAc2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.264
3GPT Crystal structure of the yeast 20S proteasome in complex with Salinosporamide derivatives: slow substrate ligand Deposited 2009-03-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa） Fragment：sequence database residues 34-266
Chain M 34–266（233 aa） Fragment：sequence database residues 34-266
Not recorded GPT (2R,3S,4R)-2-[(S)-(1S)-cyclohex-2-en-1-yl(hydroxy)methyl]-4-(2-fluoroethyl)-3-hydroxy-3-methyl-5-oxopyrrolidine-2-carbaldehyde × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；12% MPD, 0.1M MES, 20mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.41 Å R-free 0.245
3GPW Crystal structure of the yeast 20S proteasome in complex with Salinosporamide derivatives: irreversible inhibitor ligand Deposited 2009-03-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa） Fragment：sequence database residues 34-266
Chain M 34–266（233 aa） Fragment：sequence database residues 34-266
Not recorded SA1 (3AR,6R,6AS)-6-((S)-((S)-CYCLOHEX-2-ENYL)(HYDROXY)METHYL)-6A-METHYL-4-OXO-HEXAHYDRO-2H-FURO[3,2-C]PYRROLE-6-CARBALDEHYDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.7；12% MPD, 0.1M MES, 20mM MgAc2, pH 6.7, VAPOR DIFFUSION, HANGING DROP
Resolution 2.50 Å R-free 0.255
3HYE Crystal structure of 20S proteasome in complex with hydroxylated salinosporamide Deposited 2009-06-22 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa）
Chain M 34–266（233 aa）
Not recorded HYE (2R,3S,4R)-2-[(S)-(1S)-cyclohex-2-en-1-yl(hydroxy)methyl]-3-hydroxy-4-(2-hydroxyethyl)-3-methyl-5-oxopyrrolidine-2-carbaldehyde × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；293 K；Crystals grew within one week under 0.1M MES/HCl,pH 6.6, 30mM MgAc2 and 15% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å R-free 0.242
3JCO Structure of yeast 26S proteasome in M1 state derived from Titan dataset Deposited 2016-01-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain 2 1–266（266 aa）
Chain 9 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 50mM Tris pH 7.5, 100mM NaCl, 5mM MgCl2, 2mM ATP；pH 7.5；50mM Tris pH 7.5, 100mM NaCl, 5mM MgCl2, 2mM ATP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.80 Å
3JCP Structure of yeast 26S proteasome in M2 state derived from Titan dataset Deposited 2016-01-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain 2 1–266（266 aa）
Chain 9 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 50mM Tris pH 7.5, 100mM NaCl, 5mM MgCl2, 2mM ATP；pH 7.5；50mM Tris pH 7.5, 100mM NaCl, 5mM MgCl2, 2mM ATP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
3MG0 Structure of yeast 20S proteasome with bortezomib Deposited 2010-04-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa）
Chain M 34–266（233 aa）
Not recorded BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, pH 7.0, vapor diffusion, hanging drop, temperature 293K
Resolution 2.68 Å R-free 0.253
3MG4 Structure of yeast 20S proteasome with Compound 1 Deposited 2010-04-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa）
Chain M 34–266（233 aa）
Not recorded MG MAGNESIUM ION × 10 LXT (2S)-2-amino-N-[(1S)-1-({(1S)-1-[(4-methylbenzyl)carbamoyl]-3-phenylpropyl}carbamoyl)-3-phenylpropyl]-4-phenylbutanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, pH 7.0, vapor diffusion, hanging drop, temperature 293K
Resolution 3.11 Å R-free 0.250
3MG6 Structure of yeast 20S open-gate proteasome with Compound 6 Deposited 2010-04-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 1–266（266 aa）
Chain M 1–266（266 aa）
Not recorded MG MAGNESIUM ION × 10 LZT N~2~-{[(1S)-6-methoxy-3-oxo-2,3-dihydro-1H-inden-1-yl]acetyl}-N-{(1S)-1-[(4-methylbenzyl)carbamoyl]-3-phenylpropyl}-L-threoninamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, pH 7.0, vapor diffusion, hanging drop, temperature 293K
Resolution 2.60 Å R-free 0.249
3MG7 Structure of yeast 20S open-gate proteasome with Compound 8 Deposited 2010-04-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 1–266（266 aa）
Chain M 1–266（266 aa）
Not recorded MG MAGNESIUM ION × 10 L2T 4-(benzyloxy)-N-[(1S,2R)-2-hydroxy-1-({(1S)-1-[(2-methylbenzyl)carbamoyl]-3-phenylpropyl}carbamoyl)propyl]benzamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, pH 7.0, vapor diffusion, hanging drop, temperature 293K
Resolution 2.78 Å R-free 0.259
3MG8 Structure of yeast 20S open-gate proteasome with Compound 16 Deposited 2010-04-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 1–266（266 aa）
Chain M 1–266（266 aa）
Not recorded MG MAGNESIUM ION × 10 L3T N-(2,2-dimethylpropyl)-N~2~-[1H-indol-3-yl(oxo)acetyl]-L-asparaginyl-N-(2-methylbenzyl)-3-pyridin-4-yl-L-alaninamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, pH 7.0, vapor diffusion, hanging drop, temperature 293K
Resolution 2.59 Å R-free 0.252
3NZJ Crystal structure of yeast 20S proteasome in complex with ligand 2a Deposited 2010-07-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain 1 1–266（266 aa）
Chain M 1–266（266 aa）
Not recorded MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；293 K；1000 mM MES / HCl, 6.8, 12% MPD, 20 mM MgAc2, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.40 Å R-free 0.249
3NZW Crystal structure of the yeast 20S proteasome in complex with 2b Deposited 2010-07-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain 1 1–266（266 aa）
Chain M 1–266（266 aa）
Not recorded MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；100 mM MES/HCl, 6.8, 20mM MgAc2, 12% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å R-free 0.240
3NZX Crystal structure of the yeast 20S proteasome in complex with ligand 2c Deposited 2010-07-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain 1 1–266（266 aa）
Chain M 1–266（266 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；100 mM MES/Hcl, pH 6.8, 20 mM MGAc2, 12 % MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.248
3OEU Structure of yeast 20S open-gate proteasome with Compound 24 Deposited 2010-08-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa）
Chain M 34–266（233 aa）
Not recorded MG MAGNESIUM ION × 9 OEU N-{(2S)-1-[(2-chlorobenzyl)amino]-1-oxo-4-phenylbutan-2-yl}-N~2~-[3-(2-methylphenyl)propanoyl]-L-threoninamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, pH 7.0, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, vapor diffusion, hanging drop, temperature 293K
Resolution 2.60 Å R-free 0.249
3OEV Structure of yeast 20S open-gate proteasome with Compound 25 Deposited 2010-08-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa）
Chain M 34–266（233 aa）
Not recorded MG MAGNESIUM ION × 10 3OE 4-(benzyloxy)-N-[(2S,3R)-3-hydroxy-1-{[(2S)-1-{[(3-methylthiophen-2-yl)methyl]amino}-1-oxo-4-phenylbutan-2-yl]amino}-1-oxobutan-2-yl]benzamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, pH 7.0, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, vapor diffusion, hanging drop, temperature 293K
Resolution 2.85 Å R-free 0.257
3OKJ Alpha-keto-aldehyde binding mechanism reveals a novel lead structure motif for proteasome inhibition Deposited 2010-08-25 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa） Fragment：sequence database residues 34-266
Chain M 34–266（233 aa） Fragment：sequence database residues 34-266
Not recorded EP9 N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S,3S)-3-hydroxy-1-(4-hydroxyphenyl)-4-oxobutan-2-yl]-L-leucinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；293 K；Crystalls appeared during one week using 100mM MES, 20mM MG2Ac, 12% MPD, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.259
3SDI Structure of yeast 20S open-gate proteasome with Compound 20 Deposited 2011-06-09 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa）
Chain M 34–266（233 aa）
Not recorded MG MAGNESIUM ION × 20 3SD N~4~-(2,2-dimethylpropyl)-N~1~-{(2S)-1-[(4-methylbenzyl)amino]-1-oxo-4-phenylbutan-2-yl}-N~2~-[(5-methyl-1,2-oxazol-3-yl)carbonyl]-L-aspartamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, pH 7.0, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, vapor diffusion, hanging drop, temperature 293K
Resolution 2.65 Å R-free 0.253
3SDK Structure of yeast 20S open-gate proteasome with Compound 34 Deposited 2011-06-09 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa）
Chain M 34–266（233 aa）
Not recorded MG MAGNESIUM ION × 10 P3N N-[(2S)-3-(3-tert-butyl-1,2,4-oxadiazol-5-yl)-1-({(2S)-1-[(4-methylbenzyl)amino]-1-oxo-4-phenylbutan-2-yl}amino)-1-oxopropan-2-yl]-5-methyl-1,2-oxazole-3-carboxamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；100 mM MES, pH 7.0, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, vapor diffusion, hanging drop, temperature 293K
Resolution 2.70 Å R-free 0.259
3SHJ Proteasome in complex with hydroxyurea derivative HU10 Deposited 2011-06-16 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa）
Chain M 34–266（233 aa）
Not recorded MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 H10 1-hydroxy-1-[(2R)-4-{3-[(3S,5S,7S)-tricyclo[3.3.1.1~3,7~]dec-1-yloxy]phenyl}but-3-yn-2-yl]urea × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20 mM magnesium acetate, 11% PEG, 100 mM MES/NaOH, pH 7.0, crystals grow to their final size within a week, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.266
3TDD Crystal structure of yeast CP in complex with Belactosin C Deposited 2011-08-10 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 1 34–266（233 aa）
Chain M 34–266（233 aa）
Not recorded BFO benzyl N-[(naphthalen-2-ylmethoxy)carbonyl]-L-alanyl-N~5~-[(2R,3S,4S)-3-formyl-2-hydroxy-4-methylhexanoyl]-L-ornithinate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；100 mM MES, 12% MPD, 20 mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.236
3UN4 Yeast 20S proteasome in complex with PR-957 (morpholine) Deposited 2011-11-15 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；100 mM MES, 12% MPD, 20 mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.40 Å R-free 0.219
3UN8 Yeast 20S proteasome in complex with PR-957 (epoxide) Deposited 2011-11-15 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded 049 2-(acetylamino)-4,5-anhydro-1,2-dideoxy-4-methyl-1-phenyl-D-xylitol × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；100 mM MES, 12% MPD, 20 mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.240
3WXR Yeast 20S proteasome with a mutation of alpha7 subunit Deposited 2014-08-06 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 2 1–266（266 aa）
Chain N 1–266（266 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；14% MPD, 0.1M MES, 50mM Magnesium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.15 Å R-free 0.242
4CR2 Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome Deposited 2014-02-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 33 PDB declaration: 33-meric
Chain 7 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.1
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- HOMEMADE PLUNGER,
Resolution 7.70 Å
4CR3 Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome Deposited 2014-02-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 33 PDB declaration: 33-meric
Chain 7 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.1
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- HOMEMADE PLUNGER,
Resolution 9.30 Å
4CR4 Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome Deposited 2014-02-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 33 PDB declaration: 33-meric
Chain 7 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.1
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- HOMEMADE PLUNGER,
Resolution 8.80 Å
4EU2 Crystal structure of 20s proteasome with novel inhibitor K-7174 Deposited 2012-04-25 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 2 34–266（233 aa）
Chain N 34–266（233 aa）
Not recorded WPI 1,4-bis[(4E)-5-(3,4,5-trimethoxyphenyl)pent-4-en-1-yl]-1,4-diazepane × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；293 K；4.5% (v/v) 2-methyl1-2,4-pentanediol(MPD), 36mM magnesium acetate, 90mM MES pH7.2, 10%(v/v) DMSO, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.51 Å R-free 0.255
4FZC 20S yeast proteasome in complex with cepafungin I Deposited 2012-07-06 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；100 mM MES; 20 mM MgAc2; 12% MPD, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.244
4FZG 20S yeast proteasome in complex with glidobactin Deposited 2012-07-06 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；100 mM MES; 20 mM MgAc2; 12% , pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.00 Å R-free 0.226
4G4S Structure of Proteasome-Pba1-Pba2 Complex Deposited 2012-07-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain N 34–266（233 aa）
Not recorded MG MAGNESIUM ION × 16 LDZ N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S)-4-methyl-1-oxopentan-2-yl]-L-leucinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.5；277 K；0.1 M sodium citrate, 18-20% PEG 3000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 2.49 Å R-free 0.232
4GK7 yeast 20S proteasome in complex with the Syringolin-Glidobactin chimera Deposited 2012-08-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；0.1M MES, 20mM MgAc2; 12% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.241
4HNP Crystal structure of yeast 20S proteasome in complex with vinylketone carmaphycin analogue VNK1 Deposited 2012-10-20 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded ONK N-hexanoyl-L-valyl-N~1~-[(3S,4S)-3-hydroxy-2,6-dimethylhept-1-en-4-yl]-N~5~,N~5~-dimethyl-L-glutamamide × 2 VNK N-hexanoyl-L-valyl-N~1~-[(3S,4S)-3-hydroxy-2,6-dimethylheptan-4-yl]-N~5~,N~5~-dimethyl-L-glutamamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.5；297 K；30 mM of magnesium acetate, 100 mM of MES (pH 7.2) and 12% of MPD, vapor diffusion, temperature 297K
Resolution 2.80 Å R-free 0.239
4HRC Crystal structure of yeast 20S proteasome in complex with epoxyketone carmaphycin analogue 3 Deposited 2012-10-27 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded OV2 N-hexanoyl-L-valyl-N~1~-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-N~5~,N~5~-dimethyl-L-glutamamide × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.5；297 K；30 mM of magnesium acetate, 100 mM of MES (pH 7.2) and 12% of MPD, vapor diffusion, temperature 297K
Resolution 2.80 Å R-free 0.243
4HRD Crystal structure of yeast 20S proteasome in complex with the natural product carmaphycin A Deposited 2012-10-27 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded OV1 N-[(2S)-1-({(2S)-1-{[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]amino}-4-[(R)-methylsulfinyl]-1-oxobutan-2-yl}amino)-3-methyl-1-oxobutan-2-yl]hexanamide × 6 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；297 K；30 mM of magnesium acetate, 100 mM of MES (pH 7.2) and 12% of MPD, vapor diffusion, temperature 297K
Resolution 2.80 Å R-free 0.239
4INR Yeast 20S proteasome in complex with the vinyl sulfone LU102 Deposited 2013-01-06 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded 1G1 N3Phe-Leu-Leu-Phe(4-NH2CH2)-methyl vinyl sulfone, bound form × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM magnesium acetate, 0.1 M MES, 12% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.231
4INT Yeast 20S proteasome in complex with the vinyl sulfone LU122 Deposited 2013-01-06 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded 1G5 HMB-Val-Ser-Phe(4-NH2CH2)-methyl vinyl sulfone, bound form × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM magnesium acetate, 0.1 M MES, 12% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.90 Å R-free 0.230
4INU Yeast 20S proteasome in complex with the vinyl sulfone LU112 Deposited 2013-01-06 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded 1G6 N3Phe-Phe(4-NH2CH2)-Leu-Phe(4-NH2CH2)-methyl vinyl sulfone, bound form × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；293 K；20 mM magnesium acetate, 0.1 M MES, 12% MPD, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.10 Å R-free 0.238
4J70 Yeast 20S proteasome in complex with the belactosin derivative 3e Deposited 2013-02-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded 1KR benzyl [(2S)-1-({(2R)-1-[(1S,2S)-2-{[(2R,3S,4S)-3-formyl-2-hydroxy-4-methylhexanoyl]amino}cyclopropyl]-4-phenylbutan-2-yl}amino)-1-oxopropan-2-yl]carbamate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；293 K；20 mM magnesium acetate, 0.1 M MES, 12% MPD, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.224
4JSQ Yeast 20S proteasome in complex with the dimerized linear mimetic of TMC-95A - yCP:4e Deposited 2013-03-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mM MgAc2, 0.1M MES, 12% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.241
4JSU Yeast 20S proteasome in complex with the dimerized linear mimetic of TMC-95A - yCP:3a Deposited 2013-03-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mM MgAc2, 0.1M MES, 12% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.90 Å R-free 0.227
4JT0 Yeast 20S proteasome in complex with the dimerized linear mimetic of TMC-95A - yCP:4a Deposited 2013-03-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 0L1 hexanedioic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20mM MgAc2, 0.1M MES, 12% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.10 Å R-free 0.223
4LQI Yeast 20S Proteasome in complex with Vibralactone Deposited 2013-07-18 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded 1Y9 vibralactone, bound form × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20 mM magnesium acetate, 100 mM MES, 12% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.227
4LTC Crystal structure of yeast 20S proteasome in complex with enone carmaphycin analogue 6 Deposited 2013-07-23 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded EC6 N-hexanoyl-L-valyl-N~1~-[(4S,5S,6R)-5-hydroxy-2,6-dimethyloctan-4-yl]-N~5~,N~5~-dimethyl-L-glutamamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.5；297 K；30 mM of magnesium acetate, 100 mM of MES (pH 7.2) and 12% of MPD, vapor diffusion, temperature 297K
Resolution 2.50 Å R-free 0.233
4NNN yCP in complex with MG132 Deposited 2013-11-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 ALD N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S)-1-hydroxy-4-methylpentan-2-yl]-L-leucinamide × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å R-free 0.249
4NNW yCP in complex with Z-Leu-Leu-Leu-ketoaldehyde Deposited 2013-11-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 2MK N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2R,3S)-1,2-dihydroxy-5-methylhexan-3-yl]-L-leucinamide × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 100 mM MES, 13% MPD , pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.60 Å R-free 0.208
4NO1 yCP in complex with Z-Leu-Leu-Leu-B(OH)2 Deposited 2013-11-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CIX N-[(benzyloxy)carbonyl]-L-leucyl-N-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-L-leucinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 100 mM MES, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å R-free 0.214
4NO6 yCP in complex with Z-Leu-Leu-Leu-vinylsulfone Deposited 2013-11-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 2M1 N-[(benzyloxy)carbonyl]-L-leucyl-N-[(3S)-5-methyl-1-(methylsulfonyl)hexan-3-yl]-L-leucinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 100 mM MES, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.00 Å R-free 0.207
4NO8 yCP in complex with Z-Leu-Leu-Leu-ketoamide Deposited 2013-11-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 2LV N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S,3S)-2-hydroxy-5-methyl-1-oxo-1-(phenylamino)hexan-3-yl]-L-leucinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 100 mM MES, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.217
4NO9 yCP in complex with Z-Leu-Leu-Leu-epoxyketone Deposited 2013-11-19 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 2L0 N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-leucinamide × 3 2LR N-[(benzyloxy)carbonyl]-L-leucyl-N-{(1R,2S)-1-hydroxy-4-methyl-1-[(2R)-2-methyloxiran-2-yl]pentan-2-yl}-L-leucinamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 100 mM MES, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.90 Å R-free 0.203
4Q1S Yeast 20S proteasome in Complex with Kendomycin Deposited 2014-04-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded 2YD (5R,6R,7S,8R,9R,12S,13E,16S,18S,19R,20aR)-4,7,19-trihydroxy-2,6,8,12,14,16,18-heptamethyl-6,7,8,9,10,11,12,15,16,17,18,19,20,20a-tetradecahydro-1,19:5,9-diepoxybenzo[18]annulen-3(5H)-one × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；20 mM MgAc2, 100 mM MES, 13% MPD, pH 6,8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.60 Å R-free 0.224
4QBY yCP in complex with BOC-ALA-ALA-ALA-CHO Deposited 2014-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain M 21–266（246 aa） Fragment：beta subunit
Chain a 21–266（246 aa） Fragment：beta subunit
Mutation：wild type Mutation：wild type MG MAGNESIUM ION × 11 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 100 mM MES, 13% MPD , pH 6.8 , VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.00 Å R-free 0.207
4QLQ yCP in complex with tripeptidic epoxyketone inhibitor 8 Deposited 2014-06-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa） Fragment：UNP residues 21-260
Chain a 21–266（246 aa） Fragment：UNP residues 21-260
Not recorded MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 38N N-(morpholin-4-ylacetyl)-L-alanyl-N-[(2S,4R)-1-cyclohexyl-5-hydroxy-4-methyl-3-oxopentan-2-yl]-O-methyl-L-tyrosinamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20mM MgAc2, 100mM MES, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.40 Å R-free 0.215
4QLS yCP in complex with tripeptidic epoxyketone inhibitor 11 Deposited 2014-06-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa） Fragment：UNP residues 21-260
Chain a 21–266（246 aa） Fragment：UNP residues 21-260
Not recorded MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 37Y N-(morpholin-4-ylacetyl)-D-alanyl-N-[(2S,4R)-1-cyclohexyl-5-hydroxy-4-methyl-3-oxopentan-2-yl]-O-methyl-L-tyrosinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20mM MGAC2, 13% MPD, PH 6.8, 100mM MES, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.199
4QLT yCP in complex with tripeptidic epoxyketone inhibitor 2 (PR924) Deposited 2014-06-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa） Fragment：UNP residues 21-260
Chain a 21–266（246 aa） Fragment：UNP residues 21-260
Not recorded MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 39V N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-L-tryptophanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MGAC2, 13% MPD, PH 6.8, 100 mM MES, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.202
4QLU yCP in complex with tripeptidic epoxyketone inhibitor 9 Deposited 2014-06-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa） Fragment：UNP residues 21-260
Chain a 21–266（246 aa） Fragment：UNP residues 21-260
Not recorded MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 38X N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-1-cyclohexyl-5-hydroxy-4-methyl-3-oxopentan-2-yl]-L-tryptophanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20mM MGAC2, 13% MPD, 100mM MES, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.248
4QLV yCP in complex with tripeptidic epoxyketone inhibitor 17 Deposited 2014-06-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 39Q N-(morpholin-4-ylacetyl)-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-O-methyl-L-tyrosinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20mM MGAC2, 13% MPD, 100mM MES, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.90 Å R-free 0.209
4QUX yCP beta5-A49T-mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.197
4QUY yCP beta5-A49S-mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.226
4QV0 yCP beta5-A49T-A50V-double mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 6 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.10 Å R-free 0.197
4QV1 yCP beta5-M45A mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.50 Å R-free 0.216
4QV3 yCP beta5-M45V mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.211
4QV4 yCP beta5-M45T mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.70 Å R-free 0.213
4QV5 yCP beta5-M45I mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.70 Å R-free 0.217
4QV6 yCP beta5-A49V mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.231
4QV7 yCP beta5-A50V mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.60 Å R-free 0.214
4QV8 yCP beta5-C52F mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.221
4QV9 yCP beta5-C63F mutant Deposited 2014-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.60 Å R-free 0.218
4QVL yCP in complex with bortezomib Deposited 2014-07-15 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.215
4QVM yCP beta5-M45A mutant in complex with bortezomib Deposited 2014-07-15 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.215
4QVN yCP beta5-M45V mutant in complex with bortezomib Deposited 2014-07-15 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.213
4QVP yCP beta5-M45T mutant in complex with bortezomib Deposited 2014-07-15 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.30 Å R-free 0.225
4QVQ yCP beta5-M45I mutant in complex with bortezomib Deposited 2014-07-15 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.60 Å R-free 0.219
4QVV yCP beta5-A49V mutant in complex with bortezomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.235
4QVW yCP beta5-A49S-mutant in complex with bortezomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.209
4QVY yCP beta5-A49T-mutant in complex with bortezomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.51 Å R-free 0.219
4QW0 yCP beta5-A49T-A50V-double mutant in complex with bortezomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.211
4QW1 yCP beta5-A50V mutant in complex with bortezomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.216
4QW3 yCP beta5-C63F mutant in complex with bortezomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 5 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.217
4QW4 yCP in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.216
4QW5 yCP beta5-M45A mutant in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 5 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.201
4QW6 yCP beta5-M45V mutant in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 6 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.206
4QW7 yCP beta5-M45T mutant in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.70 Å R-free 0.226
4QWF yCP beta5-M45I mutant in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.203
4QWG yCP beta5-A49V mutant in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 6 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.60 Å R-free 0.243
4QWI yCP beta5-A49S-mutant in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.60 Å R-free 0.214
4QWJ yCP beta5-A49T-mutant in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.228
4QWK yCP beta5-A49T-A50V-double mutant in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.209
4QWL yCP beta5-A50V mutant in complex with carfilzomib Deposited 2014-07-16 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.60 Å R-free 0.219
4QWR yCP beta5-C52F mutant in complex with carfilzomib Deposited 2014-07-17 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.209
4QWS yCP beta5-C63F mutant in complex with carfilzomib Deposited 2014-07-17 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 MG MAGNESIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.214
4QWU yCP beta5-C52F mutant in complex with bortezomib Deposited 2014-07-17 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.218
4QWX yCP in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-17 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K, pH 6.8
Resolution 2.90 Å R-free 0.212
4QXJ yCP beta5-M45A mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-21 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 6 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.222
4QZ0 yCP beta5-M45V mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 6 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.201
4QZ1 yCP beta5-M45T mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 MG MAGNESIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.216
4QZ2 yCP beta5-M45I mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.70 Å R-free 0.219
4QZ3 yCP beta5-A49V mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.207
4QZ4 yCP beta5-A49S mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.218
4QZ5 yCP beta5-A49T-mutant in complex with ONX 0914 Deposited 2014-07-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.80 Å R-free 0.224
4QZ6 yCP beta5-A49T-A50V double mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.204
4QZ7 yCP beta5-A50V mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.213
4QZW yCP beta5-C52F mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-29 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 3.00 Å R-free 0.218
4QZX yCP beta5-C63F mutant in complex with the epoxyketone inhibitor ONX 0914 Deposited 2014-07-29 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.60 Å R-free 0.223
4QZZ yCP in complex with Omuralide Deposited 2014-07-29 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 SLA Omuralide, open form × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.208
4R00 yCP beta5-C52F mutant in complex with Omuralide Deposited 2014-07-29 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 5 SLA Omuralide, open form × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.80 Å R-free 0.210
4R02 yCP in complex with BSc4999 (alpha-Keto Phenylamide) Deposited 2014-07-29 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 11 3E5 N-[(benzyloxy)carbonyl]-L-leucyl-N-{(2S,3S)-1-[(2,4-dimethylphenyl)amino]-2-hydroxy-5-methyl-1-oxohexan-3-yl}-L-leucinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.50 Å R-free 0.207
4R17 Ligand-induced aziridine-formation at subunit beta5 of the yeast 20S proteasome Deposited 2014-08-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 11 3K4 (2S,3S)-3-methylaziridine-2-carboxylic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.10 Å R-free 0.198
4R18 Ligand-induced Lys33-Thr1 crosslinking at subunit beta5 of the yeast 20S proteasome Deposited 2014-08-04 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 11 ABA ALPHA-AMINOBUTYRIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.40 Å R-free 0.195
4RUR Yeast 20S proteasome in complex with the alkaloid indolo-phakellin (4) Deposited 2014-11-21 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 3WE (2E,3aR,14aS)-9-bromo-2-imino-1,2,3,5,6,14a-hexahydro-4H,8H-imidazo[4',5':5,6]pyrrolo[1',2':4,5]pyrazino[1,2-a]indol-8-one × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；297 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K, temperature 297K
Resolution 2.50 Å R-free 0.213
4V7O Proteasome Activator Complex Deposited 2009-12-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain A4 34–266（233 aa）
Chain AR 34–266（233 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
Resolution 3.00 Å R-free 0.250
4V7O Proteasome Activator Complex Deposited 2009-12-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain B2 34–266（233 aa）
Chain BN 34–266（233 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
Resolution 3.00 Å R-free 0.250
4X6Z Yeast 20S proteasome in complex with PR-VI modulator Deposited 2014-12-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain 2 1–266（266 aa）
Chain N 1–266（266 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 9 GOL GLYCEROL × 38 MG MAGNESIUM ION × 56 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；30mM magnesium acetate, 12% MPD, 0.1M MES pH 7.2
Resolution 2.70 Å R-free 0.222
4Y69 Yeast 20S proteasome in complex with Ac-PAD-ep Deposited 2015-02-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.90 Å R-free 0.217
4Y6A Yeast 20S proteasome beta2-H114D mutant in complex with Ac-PAD-ep Deposited 2015-02-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
Resolution 2.60 Å R-free 0.232
4Y6V Yeast 20S proteasome in complex with Ac-PAE-ep Deposited 2015-02-13 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.209
4Y6Z Yeast 20S proteasome in complex with Ac-PAL-ep Deposited 2015-02-13 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.216
4Y70 Yeast 20S proteasome in complex with Ac-LAV-ep Deposited 2015-02-13 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.40 Å R-free 0.226
4Y74 Yeast 20S proteasome in complex with Ac-LAL-ep Deposited 2015-02-13 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.214
4Y75 Yeast 20S proteasome in complex with Ac-PAF-ep Deposited 2015-02-13 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.215
4Y77 Yeast 20S proteasome in complex with Ac-LAF-ep Deposited 2015-02-13 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.215
4Y78 Yeast 20S proteasome in complex with Ac-LAD-ep Deposited 2015-02-13 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.219
4Y7W Yeast 20S proteasome in complex with Ac-LAE-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.217
4Y7X Yeast 20S proteasome in complex with Ac-PAA-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.225
4Y7Y Yeast 20S proteasome in complex with Ac-LAA-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.40 Å R-free 0.235
4Y80 Yeast 20S proteasome in complex with Ac-LAI-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.219
4Y81 Yeast 20S proteasome in complex with Ac-PAY-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.234
4Y82 Yeast 20S proteasome in complex with Ac-LAY-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.207
4Y84 Yeast 20S proteasome in complex with N3-A(4,4-F2P)nLL-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.203
4Y8G Yeast 20S proteasome in complex with N3-APnLL-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.208
4Y8H Yeast 20S proteasome in complex with N3-APAL-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.214
4Y8I Yeast 20S proteasome in complex with Ac-PLL-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.220
4Y8J Yeast 20S proteasome in complex with Ac-LLL-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.224
4Y8K Yeast 20S proteasome in complex with H-APLL-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.236
4Y8L Yeast 20S proteasome in complex with Ac-APLL-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.40 Å R-free 0.224
4Y8M Yeast 20S proteasome beta7-delta7_Cter mutant Deposited 2015-02-16 Different construct Different mutation/modification Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–259（239 aa）
Chain a 21–259（239 aa）
Mutation：last seven amino acids form the C-terminus have been removed Mutation：last seven amino acids form the C-terminus have been removed MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.218
4Y8N Yeast 20S proteasome beta7-delta7_Cter mutant in complex with Ac-PAE-ep Deposited 2015-02-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain M 21–259（239 aa）
Chain a 21–259（239 aa）
Mutation：Last seven amino acids form the C-terminus have been removed Mutation：Last seven amino acids form the C-terminus have been removed MG MAGNESIUM ION × 7 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.215
4Y8O Yeast 20S proteasome beta7-delta7_Cter mutant in complex with Ac-PAF-ep Deposited 2015-02-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain M 21–259（239 aa） Fragment：UNP residues 21-259
Chain a 21–259（239 aa） Fragment：UNP residues 21-259
Mutation：Last seven amino acids form the C-terminus have been removed Mutation：Last seven amino acids form the C-terminus have been removed MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.231
4Y8P Yeast 20S proteasome beta7-delta7_Cter mutant in complex with Ac-PAL-ep Deposited 2015-02-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–259（239 aa） Fragment：UNP residues 21-259
Chain a 21–259（239 aa） Fragment：UNP residues 21-259
Mutation：Last seven amino acids form the C-terminus have been removed Mutation：Last seven amino acids form the C-terminus have been removed MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.220
4Y8Q Yeast 20S proteasome beta7-delta7_Cter mutant in complex with Ac-PAY-ep Deposited 2015-02-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain M 21–259（239 aa）
Chain a 21–259（239 aa）
Mutation：Last seven amino acids form the C-terminus have been removed Mutation：Last seven amino acids form the C-terminus have been removed MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.228
4Y8R Yeast 20S proteasome beta2-H116D mutant Deposited 2015-02-16 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.222
4Y8S Yeast 20S proteasome beta2-H116D mutant in complex with Ac-LAE-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.221
4Y8T Yeast 20S proteasome beta2-H116D mutant in complex with Ac-PAE-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.209
4Y8U Yeast 20S proteasome beta2-H116D mutant in complex with Ac-PAD-ep Deposited 2015-02-16 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 6 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.214
4Y9Y Yeast 20S proteasome beta2-H116E mutant Deposited 2015-02-17 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 10 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.203
4Y9Z Yeast 20S proteasome beta2-H116E mutant in complex with Ac-LAE-ep Deposited 2015-02-17 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.218
4YA0 Yeast 20S proteasome beta2-H116E mutant in complex with Ac-PAE-ep Deposited 2015-02-17 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.216
4YA1 Yeast 20S proteasome beta2-H116N mutant Deposited 2015-02-17 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded CL CHLORIDE ION × 4 MG MAGNESIUM ION × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.218
4YA2 Yeast 20S proteasome beta2-H116N mutant in complex with Ac-LAE-ep Deposited 2015-02-17 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.205
4YA3 Yeast 20S proteasome beta2-H116N mutant in complex with Ac-PAE-ep Deposited 2015-02-17 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.220
4YA4 Yeast 20S proteasome beta2-H114D mutant Deposited 2015-02-17 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.232
4YA5 Yeast 20S proteasome beta2-H114D mutant in complex with Ac-PAE-ep Deposited 2015-02-17 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.242
4YA7 Yeast 20S proteasome beta2-H114D mutant in complex with Ac-LAE-ep Deposited 2015-02-17 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.237
4YA9 Yeast 20S proteasome beta2-H114D mutant in complex with Ac-LAD-ep Deposited 2015-02-17 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 CL CHLORIDE ION × 1 K POTASSIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.241
4Z1L Yeast 20S proteasome in complex with belactosin C derivative 3 Deposited 2015-03-27 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 4KF (2S,3S)-2-{(1R)-2-[(3,5-dimethoxybenzyl)amino]-1-hydroxy-2-oxoethyl}-3-methylpentanoic acid × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.229
5A5B Structure of the 26S proteasome-Ubp6 complex Deposited 2015-06-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric
Chain 7 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- HOMEMADE PLUNGER,
Resolution 9.50 Å
5AHJ Yeast 20S proteasome in complex with Macyranone A Deposited 2015-02-06 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 7IM N-[(2S)-3-{[(1S)-1-carboxy-2-phenylethyl]amino}-2-methyl-3-oxopropanoyl]-L-threonyl-N-[(3S,4S)-1,3-dihydroxy-6-methylheptan-4-yl]-L-allothreoninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.8；100 MM MES, PH 7.0 12% MPD 20 MM MGAC2
Resolution 2.80 Å R-free 0.219
5BOU Yeast 20S proteasome in complex with a beta1 / beta2 specific non-peptidic sulfonamide Ligand Deposited 2015-05-27 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 4UC N-[4-(acetylsulfamoyl)phenyl]-2-(4-ethoxyphenyl)quinoline-4-carboxamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.214
5BXL Yeast 20S proteasome beta2-G170A mutant Deposited 2015-06-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.214
5BXN Yeast 20S proteasome beta2-G170A mutant in complex with Bortezomib Deposited 2015-06-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.218
5CGF Yeast 20S proteasome beta5-G48C mutant Deposited 2015-07-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.201
5CGG Yeast 20S proteasome beta5-G48C mutant in complex with alpha-chloroacetamide 1 Deposited 2015-07-09 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.231
5CGH Yeast 20S proteasome beta5-G48C mutant in complex with alpha-chloroacetamide 5 Deposited 2015-07-09 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 54L pent-4-ynoic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.227
5CGI Yeast 20S proteasome beta5-G48C mutant in complex with ONX 0914 Deposited 2015-07-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.206
5CZ4 Yeast 20S proteasome at 2.3 A resolution Deposited 2015-07-31 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.30 Å R-free 0.228
5CZ5 Yeast 20S proteasome beta1-T1A mutant in complex with Carfilzomib Deposited 2015-07-31 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.222
5CZ6 Yeast 20S proteasome beta5-T1A mutant in complex with Syringolin A, propeptide expressed in trans Deposited 2015-07-31 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 SRG (2S)-2-[[(2S)-1-[[(5S,8S,9E)-2,7-dioxo-5-propan-2-yl-1,6-diazacyclododeca-3,9-dien-8-yl]amino]-3-methyl-1-oxo-butan-2-yl]carbamoylamino]-3-methyl-butanoic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.226
5CZ7 Yeast 20S proteasome beta5-T1A beta5-K81R double mutant in complex with Bortezomib, propeptide expressed in cis Deposited 2015-07-31 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 11 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.215
5CZ8 Yeast 20S proteasome beta5-L(-49)S-K33A mutant in complex with Carfilzomib Deposited 2015-07-31 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.215
5CZ9 Yeast 20S proteasome beta5-D17N mutant in complex with Carfilzomib; Propeptide expressed in trans Deposited 2015-07-31 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.223
5CZA Yeast 20S proteasome beta5-D166N mutant Deposited 2015-07-31 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.224
5D0S Yeast 20S proteasome beta5-D166N mutant in complex with Carfilzomib Deposited 2015-08-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.237
5D0T Yeast 20S proteasome beta5-D166N mutant in complex with MG132 Deposited 2015-08-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 11 CL CHLORIDE ION × 4 ALD N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S)-1-hydroxy-4-methylpentan-2-yl]-L-leucinamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.218
5D0V Yeast 20S proteasome beta5-T1C mutant in complex with Carfilzomib Deposited 2015-08-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.220
5D0W Yeast 20S proteasome beta5-T1S mutant Deposited 2015-08-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.203
5D0X Yeast 20S proteasome beta5-T1S mutant in complex with Bortezomib Deposited 2015-08-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.224
5D0Z Yeast 20S proteasome beta5-T1S mutant in complex with Carfilzomib Deposited 2015-08-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.207
5DKI Yeast 20S proteasome in complex with alkyne-PI Deposited 2015-09-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 5 5BZ [(1~{R})-1-[[(2~{S})-2-(hex-5-ynoylamino)-3-phenyl-propanoyl]amino]-3-methyl-butyl]boronic acid × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；30 mM MgAc2, 100 mM MES, 10% MPD
Resolution 2.80 Å R-free 0.219
5DKJ Yeast 20S proteasome in complex with octreotide-PI Deposited 2015-09-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 5BY {2-[2-(2-{4-[(1E)-4-{[(2S)-1-{[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]amino}-1-oxo-3-phenylpropan-2-yl]amino}-4-oxobut-1-en-1-yl]-1H-1,2,3-triazol-1-yl}ethoxy)ethoxy]ethoxy}acetic acid × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；30 mM MgAC2, 10% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.210
5FG7 Yeast 20S proteasome beta2-T1A mutant Deposited 2015-12-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.231
5FG9 Yeast 20S proteasome beta2-T(-2)V mutant Deposited 2015-12-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.230
5FGA Yeast 20S proteasome beta5-K33A mutant (propeptide expressed in trans) Deposited 2015-12-20 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.214
5FGD Yeast 20S proteasome beta5-H(-2)L-T1A double mutant in complex with Carfilzomib Deposited 2015-12-20 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 5 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.208
5FGE Yeast 20S proteasome beta5-H(-2)T-T1A double mutant in complex with Carfilzomib Deposited 2015-12-20 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.214
5FGF Yeast 20S proteasome beta5-H(-2)A-T1A-K81R triple mutant in complex with Carfilzomib Deposited 2015-12-20 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.214
5FGG Yeast 20S proteasome beta5-L(-49S)_D17N double mutant in complex with Carfilzomib Deposited 2015-12-20 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.208
5FGH Yeast 20S proteasome beta5-K33A mutant (propeptide expressed in trans) in complex with MG132 Deposited 2015-12-20 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 ALD N-[(benzyloxy)carbonyl]-L-leucyl-N-[(2S)-1-hydroxy-4-methylpentan-2-yl]-L-leucinamide × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.206
5FGI Yeast 20S proteasome beta1-T1A beta2-T1A double mutant in complex with Carfilzomib Deposited 2015-12-20 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 6 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.210
5FHS Yeast 20S proteasome beta5-K33A mutant (propeptide expressed in trans) in complex with Carfilzomib Deposited 2015-12-22 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.212
5JHR Yeast 20S proteasome in complex with the peptidic epoxyketone inhibitor 27 Deposited 2016-04-21 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 3 6KF (2S)-2-azido-N-[(2S)-3-(biphenyl-4-yl)-1-{[(2S)-1-{[(2S,3S,4R)-3,5-dihydroxy-4-methylpentan-2-yl]amino}-1-oxo-3-phenylpropan-2-yl]amino}-1-oxopropan-2-yl]-3-phenylpropanamide (non-preferred name) × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.206
5JHS Yeast 20S proteasome in complex with the peptidic epoxyketone inhibitor 15 Deposited 2016-04-21 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 6KG Nalpha-[(2S)-2-{[(2S)-2-azido-3-phenylpropanoyl]amino}-4-cyclohexylbutanoyl]-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.203
5L52 Yeast 20S proteasome in complex with epoxyketone inhibitor 14 Deposited 2016-05-27 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 6N5 ~{N}-[(2~{S})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；292 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.210
5L54 Yeast 20S proteasome in complex with epoxyketone inhibitor 16 Deposited 2016-05-27 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 79P (2~{S})-3-(1~{H}-indol-3-yl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.206
5L55 Yeast 20S proteasome in complex with epoxyketone inhibitor 18 Deposited 2016-05-27 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 11 CL CHLORIDE ION × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 6NV ~{N}-[(2~{R})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.225
5L5A Yeast 20S proteasome with human beta5i (1-138; R57T) Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.40 Å R-free 0.219
5L5B Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.211
5L5D Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with ONX 0914 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.221
5L5E Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with carfilzomib Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.227
5L5F Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with bortezomib Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.228
5L5H Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with PR-924 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 39V N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-L-tryptophanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.216
5L5I Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 9 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 38X N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-1-cyclohexyl-5-hydroxy-4-methyl-3-oxopentan-2-yl]-L-tryptophanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.210
5L5J Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 14 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 6N5 ~{N}-[(2~{S})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.200
5L5O Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 16 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 79P (2~{S})-3-(1~{H}-indol-3-yl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.214
5L5P Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 17 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 79L (2~{S})-3-(4-methoxyphenyl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.207
5L5Q Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone 18 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 6NV ~{N}-[(2~{R})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.210
5L5R Yeast 20S proteasome with human beta5i (1-138;V31M) and human beta6 (97-111; 118-133) Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.221
5L5S Yeast 20S proteasome with human beta5i (1-138; V31M) and human beta6 (97-111; 118-133) in complex with PR-924 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 39V N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-L-tryptophanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.211
5L5T Yeast 20S proteasome with human beta5i (1-138; V31M) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 16 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 79P (2~{S})-3-(1~{H}-indol-3-yl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.195
5L5U Yeast 20S proteasome with human beta5i (1-138; V31M) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 17 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 11 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.211
5L5V 'Yeast 20S proteasome with human beta5i (1-138; V31M) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 18 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 6NV ~{N}-[(2~{R})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.208
5L5W Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded CL CHLORIDE ION × 4 MG MAGNESIUM ION × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.212
5L5X Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with ONX 0914 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.215
5L5Y Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with carfilzomib Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.207
5L5Z Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with bortezomib Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.212
5L60 Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with PR-924 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 11 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 39V N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-L-tryptophanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.212
5L61 Yeast 20S proteasome with human beta5c (1-138) and human beta6 (99-132) in complex with epoxyketone inhibitor 14 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 11 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 6N5 ~{N}-[(2~{S})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.205
5L62 Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 16 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 12 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 79P (2~{S})-3-(1~{H}-indol-3-yl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.204
5L63 Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 17 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 11 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.212
5L64 Yeast 20S proteasome with human beta5c (1-138) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 18 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 6NV ~{N}-[(2~{R})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.223
5L65 Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with carfilzomib Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 3BV N-{(2S)-2-[(morpholin-4-ylacetyl)amino]-4-phenylbutanoyl}-L-leucyl-N-[(2R,3S,4S)-1,3-dihydroxy-2,6-dimethylheptan-4-yl]-L-phenylalaninamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.201
5L66 Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with bortezomib Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.210
5L67 Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with PR-924 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 39V N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-L-tryptophanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.213
5L68 Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 14 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 6N5 ~{N}-[(2~{S})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.223
5L69 Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 16 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 3 79P (2~{S})-3-(1~{H}-indol-3-yl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.217
5L6A Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 17 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 79L (2~{S})-3-(4-methoxyphenyl)-~{N}-[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{R})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.213
5L6B Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with ONX 0914 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 04C 1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.221
5L6C Yeast 20S proteasome with mouse beta5i (1-138) and mouse beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 18 Deposited 2016-05-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 6NV ~{N}-[(2~{R})-1-[[(2~{S})-3-(4-methoxyphenyl)-1-[[(2~{S},3~{S},4~{R})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]amino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-1-methyl-5~{H}-indene-2-carboxamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.217
5LAI Ligand-induced aziridine-formation at the yeast proteasomal subunit beta5 by sulfonate esters Deposited 2016-06-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 11 3K4 (2S,3S)-3-methylaziridine-2-carboxylic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAc2, 13% MPD
Resolution 2.50 Å R-free 0.219
5LAJ Ligand-induced Lys33-Thr1 crosslinking at the yeast proteasomal subunit beta5 by sulfonate esters Deposited 2016-06-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 11 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MGAC2, 13% MPD
Resolution 2.90 Å R-free 0.201
5LTT Yeast 20S proteasome with human beta5i (1-138; R57T)in complex with PR-924 Deposited 2016-09-07 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 39V N-[(3-methyl-1H-inden-2-yl)carbonyl]-D-alanyl-N-[(2S,4R)-5-hydroxy-4-methyl-3-oxo-1-phenylpentan-2-yl]-L-tryptophanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.247
5M2B Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with thiazole based inhibitor Ro19 Deposited 2016-10-12 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 7DX (2~{S})-2-cyclohexyl-4-oxidanylidene-4-[[7-(4-phenyl-1,3-thiazol-2-yl)quinolin-2-yl]amino]butanoic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.218
5MP9 26S proteasome in presence of ATP (s1) Deposited 2016-12-16 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain 7 1–266（266 aa）
Chain n 1–266（266 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.10 Å
5MPA 26S proteasome in presence of ATP (s2) Deposited 2016-12-16 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain 7 1–266（266 aa）
Chain n 1–266（266 aa）
Not recorded MG MAGNESIUM ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
5MPB 26S proteasome in presence of AMP-PNP (s3) Deposited 2016-12-16 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain 7 1–266（266 aa）
Chain n 1–266（266 aa）
Not recorded ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 7.80 Å
5MPC 26S proteasome in presence of BeFx (s4) Deposited 2016-12-16 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric
Chain 7 1–266（266 aa）
Chain n 1–266（266 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 7.70 Å
5NIF Yeast 20S proteasome in complex with Blm-pep activator Deposited 2017-03-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain 2 1–266（266 aa）
Chain N 1–266（266 aa）
Not recorded CL CHLORIDE ION × 12 MG MAGNESIUM ION × 8 EDO 1,2-ETHANEDIOL × 16 GOL GLYCEROL × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.1M MES pH 6.5, 35mM magnesium acetate, 13% MPD
Resolution 3.00 Å R-free 0.227
5WVI The resting state of yeast proteasome Deposited 2016-12-25 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain 7 1–266（266 aa）
Chain a 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
5WVK Yeast proteasome-ADP-AlFx Deposited 2016-12-25 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain 7 1–266（266 aa）
Chain a 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
6EF3 Yeast 26S proteasome bound to ubiquitinated substrate (4D motor state) Deposited 2018-08-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 24 PDB declaration: 24-meric
Chain 7 1–266（266 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE；specimens were manually blotted with Whatman #1 filter paper
Resolution 4.17 Å
6FVT 26S proteasome, s1 state Deposited 2018-03-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain 7 34–265（232 aa）
Chain n 34–265（232 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.10 Å
6FVU 26S proteasome, s2 state Deposited 2018-03-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain 7 34–265（232 aa）
Chain n 34–265（232 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
6FVV 26S proteasome, s3 state Deposited 2018-03-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain 7 34–265（232 aa）
Chain n 34–265（232 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 2 MG MAGNESIUM ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.40 Å
6FVW 26S proteasome, s4 state Deposited 2018-03-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain 7 34–265（232 aa）
Chain n 34–265（232 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
6FVX 26S proteasome, s5 state Deposited 2018-03-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain 7 34–265（232 aa）
Chain n 34–265（232 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.90 Å
6FVY 26S proteasome, s6 state Deposited 2018-03-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain 7 34–265（232 aa）
Chain n 34–265（232 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.10 Å
6G7F Yeast 20S proteasome in complex with Cystargolide B Deposited 2018-04-05 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 EPW Cystargolide B- bound form × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8
Resolution 2.70 Å R-free 0.214
6G8M Yeast 20S proteasome in complex with Cystargolide B Derivative 1 Deposited 2018-04-09 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 EQE (2~{S},3~{R})-4-[[(2~{S})-3-methyl-1-[[(2~{S})-3-methyl-1-oxidanylidene-1-phenylmethoxy-butan-2-yl]amino]-1-oxidanylidene-butan-2-yl]amino]-3-oxidanyl-4-oxidanylidene-2-propan-2-yl-butanoic acid × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8
Resolution 2.70 Å R-free 0.214
6G8N Yeast 20S proteasome in complex with Cystargolide B Derivative 2 Deposited 2018-04-09 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 EQB (2~{S},3~{S})-3-methyl-2-[(1~{R})-2-[[(2~{S})-3-methyl-1-[[(2~{S})-3-methyl-1-oxidanylidene-1-phenylmethoxy-butan-2-yl] amino]-1-oxidanylidene-butan-2-yl]amino]-1-oxidanyl-2-oxidanylidene-ethyl]pentanoic acid × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 MM MGAC2, 13% MPD, PH 6.8
Resolution 3.00 Å R-free 0.238
6GOP Yeast 20S Proteasome in complex with Homosalinosporamide A Deposited 2018-06-01 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 F6K Homosalinosporamide A - bound form × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.233
6H39 Yeast 20S proteasome in complex with the peptidic non-covalent binding inhibitor RTS-V5 Deposited 2018-07-17 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 FGY N-(2,2-dimethylpropyl)-N~2~-[4-(hydroxycarbamoyl)benzene-1-carbonyl]-L-asparaginyl-N-benzyl-L-alaninamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.217
6HTB Yeast 20S proteasome with human beta2c (S171G) Deposited 2018-10-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.220
6HTC Yeast 20S proteasome with human beta2c (S171G) in complex with ONX 0914 Deposited 2018-10-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 GQK (2~{S})-3-(4-methoxyphenyl)-~{N}-[(2~{S},3~{R})-4-methyl-3,4-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.218
6HTD Yeast 20S proteasome with human beta2c (S171G) in complex with 4 Deposited 2018-10-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQH (2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.201
6HTP Yeast 20S proteasome with human beta2c (S171G) in complex with 7 Deposited 2018-10-04 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQQ ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]pyrazine-2-carboxamide × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.206
6HTR Yeast 20S proteasome with human beta2c (S171G) in complex with 13 Deposited 2018-10-04 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 GQT (2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.225
6HUB Yeast 20S proteasome with human beta2c (S171G) in complex with 16 Deposited 2018-10-05 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GRW (2~{S})-~{N}-[(2~{S},3~{R})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-butan-2-yl]-2-[[(2~{R})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.212
6HUC Yeast 20S proteasome with human beta2c (S171G) in complex with 18 Deposited 2018-10-05 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GRT (2~{S})-~{N}-[2-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-2-oxidanylidene-ethyl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.218
6HUQ Yeast 20S proteasome with human beta2c (S171G) in complex with 20 Deposited 2018-10-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GT5 ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-methoxy-1-oxidanylidene-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-2-methyl-1,3-thiazole-5-carboxamide × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.207
6HUU Yeast 20S proteasome with human beta2c (S171G) in complex with 29 Deposited 2018-10-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GTW ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-cyclohexyl-1-oxidanylidene-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-2-methyl-1,3-thiazole-5-carboxamide × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.221
6HUV Yeast 20S proteasome with human beta2c (S171G) in complex with 39 Deposited 2018-10-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GT8 (2~{S})-~{N}-[(3~{S},4~{R})-1-cyclohexyl-5-methyl-4,5-bis(oxidanyl)hexan-3-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 6 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.10 Å R-free 0.213
6HV3 Yeast 20S proteasome with human beta2i (1-53) Deposited 2018-10-10 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.209
6HV4 Yeast 20S proteasome with human beta2i (1-53) in complex with ONX 0914 Deposited 2018-10-10 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQK (2~{S})-3-(4-methoxyphenyl)-~{N}-[(2~{S},3~{R})-4-methyl-3,4-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.214
6HV5 Yeast 20S proteasome with human beta2i (1-53) in complex with 4 Deposited 2018-10-10 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQH (2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.204
6HV7 Yeast 20S proteasome with human beta2i (1-53) in complex with 7 Deposited 2018-10-10 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQQ ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]pyrazine-2-carboxamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.40 Å R-free 0.229
6HVA Yeast 20S proteasome with human beta2i (1-53) in complex with 13 Deposited 2018-10-10 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQT (2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.208
6HVR Yeast 20S proteasome with human beta2i (1-53) in complex with 16 Deposited 2018-10-11 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GRW (2~{S})-~{N}-[(2~{S},3~{R})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-butan-2-yl]-2-[[(2~{R})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.219
6HVS Yeast 20S proteasome with human beta2i (1-53) in complex with 18 Deposited 2018-10-11 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GRT (2~{S})-~{N}-[2-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-2-oxidanylidene-ethyl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.10 Å R-free 0.205
6HVT Yeast 20S proteasome with human beta2i (1-53) in complex with 20 Deposited 2018-10-11 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GT5 ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-methoxy-1-oxidanylidene-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-2-methyl-1,3-thiazole-5-carboxamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.206
6HVU Yeast 20S proteasome with human beta2i (1-53) in complex with 29 Deposited 2018-10-11 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GTW ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-cyclohexyl-1-oxidanylidene-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-2-methyl-1,3-thiazole-5-carboxamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.220
6HVV Yeast 20S proteasome with human beta2i (1-53) in complex with 39 Deposited 2018-10-11 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GT8 (2~{S})-~{N}-[(3~{S},4~{R})-1-cyclohexyl-5-methyl-4,5-bis(oxidanyl)hexan-3-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.219
6HVW Yeast 20S proteasome with human beta2i (1-53) in complex with 43 Deposited 2018-10-11 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GVW (2~{S})-~{N}-[(2~{S},3~{R})-1-[(4~{a}~{S},8~{a}~{S})-1,2,3,4,4~{a},5,6,7,8,8~{a}-decahydronaphthalen-2-yl]-4-methyl-3,4-bis(oxidanyl)pentan-2-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.202
6HVX Yeast 20S proteasome in complex with 4 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 GQH (2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.206
6HVY Yeast 20S proteasome in complex with 5 (7- and 6-membered ring) Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GVZ (2~{S})-~{N}-[(2~{S},3~{R})-1-[(1~{S},4~{a}~{S},8~{a}~{R})-1,2,3,4,4~{a},5,6,7,8,8~{a}-decahydronaphthalen-1-yl]-4-methyl-3,4-bis(oxidanyl)pentan-2-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 2 GW2 (2~{S})-~{N}-[(2~{S},3~{S},4~{R})-1-[(1~{S},4~{a}~{S},8~{a}~{R})-1,2,3,4,4~{a},5,6,7,8,8~{a}-decahydronaphthalen-1-yl]-4-methyl-3,5-bis(oxidanyl)pentan-2-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.218
6HW0 Yeast 20S proteasome in complex with 7 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQQ ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]pyrazine-2-carboxamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.206
6HW3 Yeast 20S proteasome in complex with 13 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQT (2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.215
6HW4 Yeast 20S proteasome in complex with 16 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GRW (2~{S})-~{N}-[(2~{S},3~{R})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-butan-2-yl]-2-[[(2~{R})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.210
6HW5 Yeast 20S proteasome in complex with 18 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GRT (2~{S})-~{N}-[2-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-2-oxidanylidene-ethyl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.211
6HW6 Yeast 20S proteasome in complex with 20 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 GT5 ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-methoxy-1-oxidanylidene-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-2-methyl-1,3-thiazole-5-carboxamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.206
6HW7 Yeast 20S proteasome in complex with 29 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 GTW ~{N}-[(2~{S})-1-[[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-cyclohexyl-1-oxidanylidene-propan-2-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-2-methyl-1,3-thiazole-5-carboxamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.225
6HW8 Yeast 20S proteasome in complex with 39 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 GT8 (2~{S})-~{N}-[(3~{S},4~{R})-1-cyclohexyl-5-methyl-4,5-bis(oxidanyl)hexan-3-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.206
6HW9 Yeast 20S proteasome in complex with 41b Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 GWK (2~{S})-3-(4-methoxyphenyl)-~{N}-[(2~{S},3~{R})-4-methyl-1-(4-methylcyclohexyl)-3,4-bis(oxidanyl)pentan-2-yl]-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.205
6HWA Yeast 20S proteasome in complex with 43 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 GVW (2~{S})-~{N}-[(2~{S},3~{R})-1-[(4~{a}~{S},8~{a}~{S})-1,2,3,4,4~{a},5,6,7,8,8~{a}-decahydronaphthalen-2-yl]-4-methyl-3,4-bis(oxidanyl)pentan-2-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.216
6HWB Yeast 20S proteasome in complex with 44b Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 GWT (2~{S})-~{N}-[(2~{S},3~{R})-1-(4-cyclohexylcyclohexyl)-4-methyl-3,4-bis(oxidanyl)pentan-2-yl]-3-(4-methoxyphenyl)-2-[[(2~{S})-2-(2-morpholin-4-ium-4-ylethanoylamino)propanoyl]amino]propanamide × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.221
6HWC Yeast 20S proteasome beta2-G45A mutant Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.224
6HWD Yeast 20S proteasome beta2-G45A mutant in complex with bortezomib Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 BO2 N-[(1R)-1-(DIHYDROXYBORYL)-3-METHYLBUTYL]-N-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.211
6HWE Yeast 20S proteasome beta2-G45A mutant in complex with carfilzomib Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GWZ (2~{S})-~{N}-[(2~{S})-1-[[(3~{R},4~{S})-2,6-dimethyl-2,3-bis(oxidanyl)heptan-4-yl]amino]-1-oxidanylidene-3-phenyl-propan-2-yl]-4-methyl-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)-4-phenyl-butanoyl]amino]pentanamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.30 Å R-free 0.231
6HWF Yeast 20S proteasome beta2-G45A mutant in complex with ONX 0914 Deposited 2018-10-11 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 GQK (2~{S})-3-(4-methoxyphenyl)-~{N}-[(2~{S},3~{R})-4-methyl-3,4-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{S})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide × 6 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.50 Å R-free 0.216
6J2C Yeast proteasome in translocation competent state (C3-a) Deposited 2019-01-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain 7 1–266（266 aa）
Chain a 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.00 Å
6J2N yeast proteasome in substrate-processing state (C3-b) Deposited 2019-01-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain 7 1–266（266 aa）
Chain a 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.50 Å
6J2Q Yeast proteasome in Ub-accepted state (C1-b) Deposited 2019-01-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain 7 1–266（266 aa）
Chain a 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6J2X Yeast proteasome in resting state (C1-a) Deposited 2019-01-03 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain 7 1–266（266 aa）
Chain a 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6J30 yeast proteasome in Ub-engaged state (C2) Deposited 2019-01-03 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain 7 1–266（266 aa）
Chain a 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
6ZOU Yeast 20S proteasome in complex with glidobactin-like natural product HB333 Deposited 2020-07-07 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 QOH 11-methyl-~{N}-[(2~{S},3~{R})-1-[[(5~{S},8~{S},10~{S})-5-methyl-10-oxidanyl-2,7-bis(oxidanylidene)-1,6-diazacyclododec-8-yl]amino]-3-oxidanyl-1-oxidanylidene-butan-2-yl]dodecanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.212
6ZP6 Yeast 20S proteasome in complex with glidobactin-like natural product HB334 Deposited 2020-07-08 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 QOB ~{N}-[(2~{S},3~{R})-1-[[(5~{S},8~{S},10~{S})-5-methyl-10-oxidanyl-2,7-bis(oxidanylidene)-1,6-diazacyclododec-8-yl]amino]-3-oxidanyl-1-oxidanylidene-butan-2-yl]-5-phenyl-pentanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.202
6ZP8 Yeast 20S proteasome in complex with glidobactin-like natural product HB335 Deposited 2020-07-08 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 QOE (2~{S},3~{R})-~{N}-[(5~{S},8~{S},10~{S})-5-methyl-10-oxidanyl-2,7-bis(oxidanylidene)-1,6-diazacyclododec-8-yl]-3-oxidanyl-2-(3-phenylpropanoylamino)butanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.00 Å R-free 0.200
7LS5 Cryo-EM structure of the Pre3-1 20S proteasome core particle Deposited 2021-02-17 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 2 1–266（266 aa）
Chain N 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.74 Å
7QO3 Structure of the 26S proteasome-Ubp6 complex in the si state (Core Particle and Lid) Deposited 2021-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 41 PDB declaration: 41-meric
Chain 7 1–266（266 aa）
Chain n 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.10 Å
7QO5 26S proteasome Rpt1-RK -Ubp6-UbVS complex in the si state Deposited 2021-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric
Chain 7 1–266（266 aa）
Chain n 1–266（266 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.00 Å
7QO6 26S proteasome Rpt1-RK -Ubp6-UbVS complex in the s2 state Deposited 2021-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric
Chain 7 1–266（266 aa）
Chain n 1–266（266 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 5 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.30 Å
7TEJ Cryo-EM structure of the 20S Alpha 3 Deletion proteasome core particle Deposited 2022-01-05 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 2 1–266（266 aa）
Chain N 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.74 Å
7TEO Cryo-EM structure of the 20S Alpha 3 Deletion proteasome core particle in complex with FUB1 Deposited 2022-01-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 30 PDB declaration: 30-meric
Chain 2 1–266（266 aa）
Chain N 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.97 Å
8BW1 Yeast 20S proteasome in complex with an engineered fellutamide derivative (C14QAL) Deposited 2022-12-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 3 MYR MYRISTIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；100 mM MES, 20 mM MgAc2, 11% MPD
Resolution 3.25 Å R-free 0.212
8OHZ Yeast 20S proteasome in complex with a photoswitchable cepafungin derivative (transCep1) Deposited 2023-03-21 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 VOT (2~{S},3~{R})-2-[2-[4-[2-(4-ethylphenyl)hydrazinyl]phenyl]ethanoylamino]-~{N}-[(5~{S},8~{S},10~{S})-5-methyl-10-oxidanyl-2,7-bis(oxidanylidene)-1,6-diazacyclododec-8-yl]-3-oxidanyl-butanamide × 4 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.65 Å R-free 0.222
8OI1 Yeast 20S proteasome in complex with a photoswitchable cepafungin derivative (transCep4) Deposited 2023-03-21 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 11 VOX ~{N}-[(2~{S},3~{R})-1-[[(5~{S},8~{S},10~{S})-5-methyl-10-oxidanyl-2,7-bis(oxidanylidene)-1,6-diazacyclododec-8-yl]amino]-3-oxidanyl-1-oxidanylidene-butan-2-yl]-7-[4-[2-(4-methylphenyl)hydrazinyl]phenyl]heptanamide × 4 CL CHLORIDE ION × 2 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.95 Å R-free 0.224
8OLR Structure of yeast 20S proteasome in complex with the natural product beta-lactone inhibitor Cystargolide A Deposited 2023-03-30 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 2 VSZ Cystargolide A (bound) × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.208
8RHJ Yeast 20S proteasome in complex with a macrocyclic oxindole epoxyketone (compound 5) Deposited 2023-12-15 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.05 Å R-free 0.212
8RHK Yeast 20S proteasome in complex with a linear oxindole epoxyketone (compound 6) Deposited 2023-12-15 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.218
8RHL Yeast 20S proteasome in complex with a linear biarylether epoxyketone (compound 15a) Deposited 2023-12-15 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.20 Å R-free 0.228
8RVL Proteasomal late precursor complex from pre1-1 Deposited 2024-02-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain 2 1–266（266 aa）
Chain N 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；150 mM NaCl 50 mM Tris-HCl
cryo-EM vitrification conditions Cryogen ETHANE；manual plunge freezing device purchased from 'Neptune Fluid Flow Systems'
Resolution 2.14 Å
8RVO Proteasomal late precursor complex from pre1-1, state 1 Deposited 2024-02-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain 2 1–266（266 aa）
Chain N 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；150 mM NaCl 50 mM Tris-HCl
cryo-EM vitrification conditions Cryogen ETHANE；manual plunge freezing device purchased from 'Neptune Fluid Flow Systems'
Resolution 2.69 Å
8RVP Proteasomal late precursor complex from pre1-1, state 2 Deposited 2024-02-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain 2 1–266（266 aa）
Chain N 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；150 mM NaCl 50 mM Tris-HCl
cryo-EM vitrification conditions Cryogen ETHANE；manual plunge freezing device purchased from 'Neptune Fluid Flow Systems'
Resolution 2.28 Å
8RVQ 20S proteasome from pre1-1 Deposited 2024-02-01 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 2 34–266（233 aa）
Chain N 34–266（233 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；150 mM NaCl 50 mM Tris-HCl
cryo-EM vitrification conditions Cryogen ETHANE；manual plunge freezing device purchased from 'Neptune Fluid Flow Systems'
Resolution 2.02 Å
8T08 Preholo-Proteasome from Pre1-1 Pre4-1 Double Mutant Deposited 2023-05-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain Q 1–251（251 aa）
Chain h 1–251（251 aa）
Mutation：Deletion of 15 C-terminal residues 252-266 (WDFAKDIKGYGTQKI) Mutation：Deletion of 15 C-terminal residues 252-266 (WDFAKDIKGYGTQKI) No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8T0M Proteasome 20S core particle from Pre1-1 Pre4-1 Double mutant Deposited 2023-06-01 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain N 1–251（251 aa）
Chain b 1–251（251 aa）
Mutation：Deletion of 15 C-terminal Residues WDFAKDIKGYGTQKI Mutation：Deletion of 15 C-terminal Residues WDFAKDIKGYGTQKI No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；Fluorinated Fos-Choline was added to the sample immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
8U6Y Preholo-Proteasome from Beta 3 D205 deletion Deposited 2023-09-14 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain Q 1–266（266 aa）
Chain h 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；Fluorinated Fos-Choline was added immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8U7U Proteasome 20S Core Particle from Beta 3 D205 deletion Deposited 2023-09-15 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain 2 1–266（266 aa）
Chain N 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；Fluorinated Fos-Choline was added immediately prior to deposition on a grid for plunge freezing.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.16 Å
9AW5 Yeast 20S proteasome soaked with MA9 fraction E/F Deposited 2024-03-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded SO4 SULFATE ION × 61 MG MAGNESIUM ION × 8 A1A9B (10S,11R,12S,15S,18S)-15-(2-amino-2-oxoethyl)-10,11,23-trihydroxy-18-{[(3R)-3-methyl-2-oxopentanoyl]amino}-9,14,17-trioxo-N-[(1Z)-prop-1-en-1-yl]-8,13,16-triazatetracyclo[18.3.1.0(2,7).0(6,10)]tetracosa-1(24),2,4,6,20,22-hexaene-12-carboxamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
Resolution 3.44 Å R-free 0.252
9AW6 Yeast 20S proteasome soaked with MA9 fraction EF2 Deposited 2024-03-05 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded SO4 SULFATE ION × 58 A1A9B (10S,11R,12S,15S,18S)-15-(2-amino-2-oxoethyl)-10,11,23-trihydroxy-18-{[(3R)-3-methyl-2-oxopentanoyl]amino}-9,14,17-trioxo-N-[(1Z)-prop-1-en-1-yl]-8,13,16-triazatetracyclo[18.3.1.0(2,7).0(6,10)]tetracosa-1(24),2,4,6,20,22-hexaene-12-carboxamide × 6 MG MAGNESIUM ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM
Resolution 3.44 Å R-free 0.235
9C98 Yeast 20S proteasome soaked with isolated TMC-86A Deposited 2024-06-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 34–266（233 aa）
Chain a 34–266（233 aa）
Not recorded SO4 SULFATE ION × 69 MG MAGNESIUM ION × 12 A1AU6 N-[(2S)-3-hydroxy-1-{[(4S)-1-hydroxy-2-(hydroxymethyl)-6-methyl-3-oxoheptan-4-yl]amino}-1-oxopropan-2-yl]butanamide × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；291 K；MES 100 mM pH 6.9, MPD 25% and magnesium acetate 20 mM
Resolution 3.04 Å R-free 0.221
9CGC Yeast 26S proteasome non-substrate-engaged (S1 state) Deposited 2024-06-28 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 39 PDB declaration: 39-meric
Chain 7 1–266（266 aa）
Chain a 1–266（266 aa）
Not recorded MG MAGNESIUM ION × 5 ATP ADENOSINE-5'-TRIPHOSPHATE × 5 ZN ZINC ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；30 mM HEPES pH7.4, 25 mM NaCl, 25 mM KCl, 3% (v/v) glycerol, 5 mM MgCl2 2 mM ATP and 0.5 mM TCEP, 0.02% NP-40.
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.61 Å
9EY9 Yeast 20S proteasome in complex with a sybactin derivative (PheSyr) Deposited 2024-04-09 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 A1H71 sybactin derivative × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.10 Å R-free 0.241
9FRW Yeast 20S proteasome with human beta1i (1-51) Deposited 2024-06-19 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded CL CHLORIDE ION × 7 MG MAGNESIUM ION × 9 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.85 Å R-free 0.227
9FST Yeast 20S proteasome with human beta1i (1-51) in complex with epoxyketone inhibitor LU-001i Deposited 2024-06-21 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded CL CHLORIDE ION × 4 MG MAGNESIUM ION × 8 A1IF8 azanylidene-[2-[[(2S)-1-[(2S)-2-[[(2S)-1-[[(1S)-2-cyclohexyl-1-[(2R,3S,6R,7S)-3-methanoyl-2,6-dimethyl-6,7-bis(oxidanyl)-1,4-oxazepan-7-yl]ethyl]amino]-1-oxidanylidene-hexan-2-yl]carbamoyl]-4,4-bis(fluoranyl)pyrrolidin-1-yl]-1-oxidanylidene-propan-2-yl]amino]-2-oxidanylidene-ethyl]imino-azanium × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.75 Å R-free 0.245
9FSU Yeast 20S proteasome with human beta1i (1-51) in complex with epoxyketone inhibitor 16 Deposited 2024-06-21 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 SO4 SULFATE ION × 4 A1IFL (2S)-N-[(2S)-1-[[(1S)-2-cyclohexyl-1-[(2R,3S,6R,7S)-3-methanoyl-2,6-dimethyl-6,7-bis(oxidanyl)-1,4-oxazepan-7-yl]ethyl]amino]-3-(4-methoxyphenyl)-1-oxidanylidene-propan-2-yl]-2-(2-morpholin-4-ylethanoylamino)-4-oxidanyl-butanamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.75 Å R-free 0.245
9FSV Yeast 20S proteasome with human beta2i (1-53) in complex with epoxyketone inhibitor 16 Deposited 2024-06-22 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 3 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 A1IFL (2S)-N-[(2S)-1-[[(1S)-2-cyclohexyl-1-[(2R,3S,6R,7S)-3-methanoyl-2,6-dimethyl-6,7-bis(oxidanyl)-1,4-oxazepan-7-yl]ethyl]amino]-3-(4-methoxyphenyl)-1-oxidanylidene-propan-2-yl]-2-(2-morpholin-4-ylethanoylamino)-4-oxidanyl-butanamide × 4 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.75 Å R-free 0.247
9FT0 Yeast 20S proteasome in complex with epoxyketone inhibitor 16 Deposited 2024-06-23 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 9 CL CHLORIDE ION × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 3 A1IFL (2S)-N-[(2S)-1-[[(1S)-2-cyclohexyl-1-[(2R,3S,6R,7S)-3-methanoyl-2,6-dimethyl-6,7-bis(oxidanyl)-1,4-oxazepan-7-yl]ethyl]amino]-3-(4-methoxyphenyl)-1-oxidanylidene-propan-2-yl]-2-(2-morpholin-4-ylethanoylamino)-4-oxidanyl-butanamide × 4 SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.75 Å R-free 0.232
9FT1 Yeast 20S proteasome in complex with epoxyketone inhibitor 9 Deposited 2024-06-23 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 5 A1IFK tert-butyl 4-[2-[[(2S)-1-[[(2S)-1-[[(1S)-2-cyclohexyl-1-[(2R,3S,6R,7S)-3-methanoyl-2,6-dimethyl-6,7-bis(oxidanyl)-1,4-oxazepan-7-yl]ethyl]amino]-3-(4-methoxyphenyl)-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-3-phenylmethoxy-propan-2-yl]amino]-2-oxidanylidene-ethyl]piperazine-1-carboxylate × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.222
9GBK Blm10-20S proteasome complex from pre1-1 Deposited 2024-07-31 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 29 PDB declaration: 29-meric
Chain 2 34–266（233 aa）
Chain N 34–266（233 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；150 mM NaCl 50 mM Tris-HCl
cryo-EM vitrification conditions Cryogen ETHANE；manual plunge freezing device purchased from 'Neptune Fluid Flow Systems'
Resolution 2.39 Å
9IWR 26S proteasome trimer Deposited 2024-07-25 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric
Chain 7 1–266（266 aa）
Chain n 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 6.8；YPD buffer
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 9.10 Å
9QAF Yeast 20S proteasome mutant: beta5_T3M Deposited 2025-02-28 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.213
9QAI Yeast 20S proteasome mutant: beta5_T3M in complex with MG132 Deposited 2025-02-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 11 CL CHLORIDE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.205
9QB1 Yeast 20S proteasome mutant: beta5_T3M in complex with Bortezomib Deposited 2025-02-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.215
9QB4 Yeast 20S proteasome mutant: beta5_T3M in complex with Carfilzomib Deposited 2025-02-28 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.207
9QBE Yeast 20S proteasome mutant: beta5_T3M in complex with ONX0914 Deposited 2025-03-02 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 10 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.211
9QBI Yeast 20S proteasome mutant: beta5_G128V (b5-propeptide in trans) Deposited 2025-03-03 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded SO4 SULFATE ION × 2 MG MAGNESIUM ION × 3 CL CHLORIDE ION × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.215
9QBO Yeast 20S proteasome mutant: beta5_G128V (b5-propeptide in trans) in complex with MG132 Deposited 2025-03-03 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded SO4 SULFATE ION × 4 MG MAGNESIUM ION × 2 CL CHLORIDE ION × 4 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.212
9QBW Yeast 20S proteasome mutant: beta5_G128V (b5-propeptide in trans) in complex with Carfilzomib Deposited 2025-03-03 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 3 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 5 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.207
9QBY Yeast 20S proteasome mutant: beta5_G128V (b5-propeptide in trans) in complex with ONX0914 Deposited 2025-03-04 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 3 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.211
9QC9 Yeast 20S proteasome double mutant: beta5_F(-46)S / beta5_G128V Deposited 2025-03-04 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 6 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 8 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.60 Å R-free 0.224
9QCF Yeast 20S proteasome mutant: beta1_G128V Deposited 2025-03-04 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 8 CL CHLORIDE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.90 Å R-free 0.214
9QCK Yeast 20S proteasome mutant: beta1_G128V (b1-propeptide deleted) Deposited 2025-03-04 Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 28 PDB declaration: 28-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 5 CL CHLORIDE ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.213
9QD2 Yeast 20S proteasome mutant: beta1_G128V (b1-propeptide deleted) in complex with MG132 Deposited 2025-03-05 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 4 CL CHLORIDE ION × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.214
9QDF Yeast 20S proteasome mutant: beta1_G128V (b1-propeptide deleted) in complex with Bortezomib Deposited 2025-03-06 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 4 CL CHLORIDE ION × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.70 Å R-free 0.217
9QDS Yeast 20S proteasome mutant: beta1_G128V (b1-propeptide deleted) in complex with Carfilzomib Deposited 2025-03-06 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 34 PDB declaration: 34-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 4 CL CHLORIDE ION × 5 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 2.80 Å R-free 0.211
9S78 Yeast 20S Proteasome in Complex with Tellurophene-Tagged Carfilzomib Deposited 2025-08-04 Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric
Chain M 21–266（246 aa）
Chain a 21–266（246 aa）
Not recorded MG MAGNESIUM ION × 7 CL CHLORIDE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES
Resolution 3.15 Å R-free 0.223

358 other PDB entries and 359 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PSB7_YEAST
Isoform —
PDB entities 13
Chains and sequence ranges Author chain M； PDBConstruct 1–246； UniProt 21–266 Author chain a； PDBConstruct 1–246； UniProt 21–266

Proteasome endopeptidase complex

OrganismNot specified

UniProt A0A6L0ZSP2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 28 PDB declaration: 28-meric（28） Consistent with protein copy count Chain N； UniProt 20–215 Chain b； UniProt 20–215 Not recorded HLJ1_G0039880.mRNA.1.CDS.1 × 2 （A0A6L1BIF8） BJ4_G0021480.mRNA.1.CDS.1 × 2 （A0A6A5PXC6） HLJ1_G0048980.mRNA.1.CDS.1 × 2 （A0A6A5Q273） 20S proteasome × 2 （P32379） BJ4_G0043800.mRNA.1.CDS.1 × 2 （A0A6A5PTH4） Probable proteasome subunit alpha type-7 × 2 （A0A6A5Q4M4） BJ4_G0020160.mRNA.1.CDS.1 × 2 （A0A6A5PYC9） Proteasome endopeptidase complex × 2 （A0A6A5Q449） Proteasome endopeptidase complex × 2 （A0A6L0YA22） Proteasome subunit beta × 2 （A0A6A5Q0W2） Proteasome subunit beta type-5 × 2 （A0A6V8RU78） Proteasome endopeptidase complex × 2 （A0A6A5Q0P3） Proteasome subunit beta type-7 × 2 （P30657） MG MAGNESIUM ION × 8 CL CHLORIDE ION × 2 V08 (2 {R},3 {S})-3-methanoyl-4-methyl-2-hydroxy-pentanoic acid × 6 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.8；293 K；20 mM MgAC2, 13% MPD, 0.1 M MES Resolution 3.00 Å R-free 0.216

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence
UniProt name A0A6L0ZSP2_YEASX
Isoform —
PDB entities 14
Chains and sequence ranges Author chain N； PDBConstruct 1–196； UniProt 20–215 Author chain b； PDBConstruct 1–196； UniProt 20–215

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 7o2l

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 7o2l
Download Download
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2. Structure Basics 2. Structure Basics

Entry ID entry_id7o2l
Deposition date deposition_date2021-03-30
Structure title titleYeast 20S proteasome in complex with the covalently bound inhibitor b-lactone (2R,3S)-3-isopropyl-4-oxo-2-oxetane-carboxylate (IOC)
Keywords keywordsHYDROLASE-HYDROLASE INHIBITOR complex, Proteasome, b-lactone, Binding Analysis, HYDROLASE; HYDROLASE
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier60.42 Å
Radius of gyration Rg (electron density) rg_electron59.96 Å
Forward intensity I(0) i06788000000.00
Molecular weight molecular_weight700790.0 kDa
Excluded volume excluded_volume878950 ų
Envelope volume envelope_volume1266200 ų
Hydration-shell volume shell_volume169430 ų
Envelope diameter envelope_diameter195.2 Å
Shell Rg shell_rg68.02 Å
Envelope Rg envelope_rg57.83 Å
Shape Rg shape_rg59.95 Å
Total Rg total_rg60.17 Å
Total atoms total_atoms49366
Residues n_residues6334
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax194.9 Å
Rg (real space) rg_real60.14 Å
Rg uncertainty (real space) rg_real_error1.68 Å
I(0) (real space) i0_real6.7880e+09
I(0) uncertainty (real space) i0_real_error1.4010e+08
Rg (reciprocal space) rg_reciprocal60.64 Å
I(0) (reciprocal space) i0_reciprocal6793000000.0000
Solution quality estimate total_estimate0.7981
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary75.0 Å
Skewness Skewness skewness0.208
Kurtosis Kurtosis kurtosis-0.419
Angular range angular_range— – 0.1300 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha1076000000.0000
Real-space data points n_real_points27
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.805; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.956; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (18)

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7. Fold Classification (SCOP + CATH) 16 domains

CATH v4.4 (16 domains)

Domain ID domain_id7o2lA01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7o2lB01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7o2lC01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7o2lD01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7o2lF01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7o2lH01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7o2lK01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7o2lL01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7o2lO01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7o2lP01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7o2lQ01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7o2lR01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7o2lT01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7o2lV01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7o2lY01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
Domain ID domain_id7o2lZ01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology20 — Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homologous superfamily homologous superfamily10 — Aminohydrolase, N-terminal nucleophile (Ntn) domain
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9. Files and Curves (10)