Calmodulin-1
Homo sapiens
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count | Chain C； UniProt 1–149 | Mutation：D132E | KCNQ1 helixAB × 1 CA CALCIUM ION × 2 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；298.15 K；0.1M Tris (pH8.5), 0.2M Sodium acetate trihydrate, 32% PEG4000 | Resolution 3.14 Å R-free 0.345 |
| 2 | Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count | Chain D； UniProt 1–149 | Mutation：D132E | KCNQ1 helixAB × 1 CA CALCIUM ION × 2 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；298.15 K；0.1M Tris (pH8.5), 0.2M Sodium acetate trihydrate, 32% PEG4000 | Resolution 3.14 Å R-free 0.345 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 9KVO | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 12DK structure of human KCNQ1-CaM-PIP2 intermediate state Deposited 2026-03-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–149（149 aa）
Chain D
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 4 CA CALCIUM ION × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.47 Å |
| 22RJ Human KCNQ3-CaM in apo state Deposited 2026-01-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions | Resolution 2.82 Å |
| 2KUG Halothane binds to druggable sites in calcium-calmodulin: Solution Structure of halothane-CaM N-terminal domain Deposited 2010-02-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–149（148 aa）
|
Not recorded | CA CALCIUM ION × 2 HLT 2-BROMO-2-CHLORO-1,1,1-TRIFLUOROETHANE × 1 |
SOLUTION NMR
NMR measurement conditions
pH 7.2；298 K；Ionic strength (raw mmCIF value) 0.1；Pressure ambient
NMR sample composition
2 mM [U-99% 13C; U-99% 15N] CALMODULIN, 20 mM CALCIUM ION, 20 mM N-{[2-({[1-(4-CARBOXYBUTANOYL)AMINO]-2-PHENYLETHYL}-HYDROXYPHOSPHINYL)OXY]ACETYL}-2-PHENYLETHYLAMINE, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
2 mM [U-99% 15N] CALMODULIN, 20 mM CALCIUM ION, 20 mM N-{[2-({[1-(4-CARBOXYBUTANOYL)AMINO]-2-PHENYLETHYL}-HYDROXYPHOSPHINYL)OXY]ACETYL}-2-PHENYLETHYLAMINE, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 3EVV Crystal Structure of Calcium bound dimeric GCAMP2 (#2) Deposited 2008-10-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
5–149（145 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 8 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.60 Å R-free 0.270 |
| 3O77 The structure of Ca2+ Sensor (Case-16) Deposited 2010-07-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–149（147 aa）
|
Mutation：POINT MUTATIONS Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 SO4 SULFATE ION × 2 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；Reservoir: 50mM Imidazol, 1.9M Na2malonate pH 6.4; Protein stock solution: 50mM Tris HCl (pH 7.4), 150mM NaCl, 10mM dithiothreitol, protein 4.1mg/ml; Drop ratio reservoir/protein = 1/3, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.35 Å R-free 0.274 |
| 3O78 The structure of Ca2+ Sensor (Case-12) Deposited 2010-07-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–149（147 aa）
|
Mutation：POINT MUTATIONS Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；Reservoir: 100mM Tris HCL (pH 5.5), 100mM (NH4)2SO4, 21% PEG 3350; Protein stock: 7.6 mg/ml Protein, 50mM Tris HCl (pH 7.4), 150mM NaCl; Seed stock solution: 20mM CaCl2, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.316 |
| 3O78 The structure of Ca2+ Sensor (Case-12) Deposited 2010-07-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–149（147 aa）
|
Mutation：POINT MUTATIONS Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；Reservoir: 100mM Tris HCL (pH 5.5), 100mM (NH4)2SO4, 21% PEG 3350; Protein stock: 7.6 mg/ml Protein, 50mM Tris HCl (pH 7.4), 150mM NaCl; Seed stock solution: 20mM CaCl2, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.316 |
| 3O78 The structure of Ca2+ Sensor (Case-12) Deposited 2010-07-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–149（147 aa）
Chain B
3–149（147 aa）
|
Mutation：POINT MUTATIONS Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：POINT MUTATIONS Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；Reservoir: 100mM Tris HCL (pH 5.5), 100mM (NH4)2SO4, 21% PEG 3350; Protein stock: 7.6 mg/ml Protein, 50mM Tris HCl (pH 7.4), 150mM NaCl; Seed stock solution: 20mM CaCl2, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.316 |
| 5OEO Solution structure of the complex of TRPV5(655-725) with a Calmodulin E32Q/E68Q double mutant Deposited 2017-07-09 | Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–149（149 aa）
|
Mutation：E32Q, E68Q | CA CALCIUM ION × 2 |
SOLUTION NMR
NMR measurement conditions
pH 7.4；308 K；Ionic strength (raw mmCIF value) 84；Pressure 1
NMR sample composition
220 uM 13C/15N Calmodulin, 220 uM hTRPV5(655-725), 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
380 uM [U-99% 15N] Calmodulin, 380 uM [U-99% 13C; U-99% 15N] hTRPV5(655-725), 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 5V02 A positive allosteric modulator binding pocket in SK2 ion channels is shared by Riluzole and CyPPA Deposited 2017-02-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain R
1–149（149 aa）
|
Not recorded | SO4 SULFATE ION × 2 657 6-(trifluoromethoxy)-1,3-benzothiazol-2-amine × 1 GOL GLYCEROL × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；295 K；ammonia sulfate
|
Resolution 1.78 Å R-free 0.215 |
| 5V7X Crystal Structure of Myosin 1b residues 1-728 with bound sulfate and Calmodulin Deposited 2017-03-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–149（149 aa）
|
Not recorded | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；10% PEG 3350, 100 mM LiOOCCH3, 100 mM HEPES, pH 7.0, 20 mM Mg2SO4
|
Resolution 3.14 Å R-free 0.202 |
| 5WBX Structural insights into the potency of SK/IK channel positive modulators Deposited 2017-06-29 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain R
5–148（144 aa）
|
Not recorded | SO4 SULFATE ION × 4 GOL GLYCEROL × 2 CA CALCIUM ION × 2 AJY (3Z)-6-bromo-3-(hydroxyimino)-5-methyl-1,3-dihydro-2H-indol-2-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.8；293.15 K；0.1 M Sodium itrate tribasic dihydrate
0.5 M Ammonium sulfate
1.5 M Lithium sulfate monohydrate
|
Resolution 1.90 Å R-free 0.243 |
| 5WBX Structural insights into the potency of SK/IK channel positive modulators Deposited 2017-06-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
5–148（144 aa）
|
Not recorded | SO4 SULFATE ION × 8 GOL GLYCEROL × 4 CA CALCIUM ION × 4 AJY (3Z)-6-bromo-3-(hydroxyimino)-5-methyl-1,3-dihydro-2H-indol-2-one × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.8；293.15 K；0.1 M Sodium itrate tribasic dihydrate
0.5 M Ammonium sulfate
1.5 M Lithium sulfate monohydrate
|
Resolution 1.90 Å R-free 0.243 |
| 5WC5 Structural insights into the potency of SK/IK channel positive modulators Deposited 2017-06-29 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain R
5–148（144 aa）
|
Not recorded | SO4 SULFATE ION × 6 GOL GLYCEROL × 1 CA CALCIUM ION × 2 AJV 7-fluoro-3-(hydroxyamino)-2H-indol-2-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.8；293.15 K；0.1 M Sodium citrate tribasic dihydrate
0.5 M Ammonium sulfate
1.5 M Litium sulfate monohydrate
|
Resolution 2.30 Å R-free 0.250 |
| 5WC5 Structural insights into the potency of SK/IK channel positive modulators Deposited 2017-06-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
5–148（144 aa）
|
Not recorded | SO4 SULFATE ION × 12 GOL GLYCEROL × 2 CA CALCIUM ION × 4 AJV 7-fluoro-3-(hydroxyamino)-2H-indol-2-one × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.8；293.15 K；0.1 M Sodium citrate tribasic dihydrate
0.5 M Ammonium sulfate
1.5 M Litium sulfate monohydrate
|
Resolution 2.30 Å R-free 0.250 |
| 6B8L Crystal Structure of the Apo/CaM:Kv7.4 (KCNQ4) AB Domain Complex Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；2M ammonium sulfate, 0.1M BisTris pH 6.5
|
Resolution 2.30 Å R-free 0.246 |
| 6B8L Crystal Structure of the Apo/CaM:Kv7.4 (KCNQ4) AB Domain Complex Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–149（149 aa）
|
Not recorded | SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；2M ammonium sulfate, 0.1M BisTris pH 6.5
|
Resolution 2.30 Å R-free 0.246 |
| 6B8L Crystal Structure of the Apo/CaM:Kv7.4 (KCNQ4) AB Domain Complex Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain F
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；2M ammonium sulfate, 0.1M BisTris pH 6.5
|
Resolution 2.30 Å R-free 0.246 |
| 6B8L Crystal Structure of the Apo/CaM:Kv7.4 (KCNQ4) AB Domain Complex Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain H
1–149（149 aa）
|
Not recorded | SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；2M ammonium sulfate, 0.1M BisTris pH 6.5
|
Resolution 2.30 Å R-free 0.246 |
| 6B8M Crystal Structure of the Ca2+/CaM:Kv7.4 (KCNQ4) AB Domain Complex, 1 mM CaCl2 soak Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；2M ammonium sulfate, 0.1M BisTris pH 6.5, 1mM CaCl2
|
Resolution 2.30 Å R-free 0.262 |
| 6B8M Crystal Structure of the Ca2+/CaM:Kv7.4 (KCNQ4) AB Domain Complex, 1 mM CaCl2 soak Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；2M ammonium sulfate, 0.1M BisTris pH 6.5, 1mM CaCl2
|
Resolution 2.30 Å R-free 0.262 |
| 6B8M Crystal Structure of the Ca2+/CaM:Kv7.4 (KCNQ4) AB Domain Complex, 1 mM CaCl2 soak Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain F
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 2 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；2M ammonium sulfate, 0.1M BisTris pH 6.5, 1mM CaCl2
|
Resolution 2.30 Å R-free 0.262 |
| 6B8M Crystal Structure of the Ca2+/CaM:Kv7.4 (KCNQ4) AB Domain Complex, 1 mM CaCl2 soak Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain H
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 2 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；2M ammonium sulfate, 0.1M BisTris pH 6.5, 1mM CaCl2
|
Resolution 2.30 Å R-free 0.262 |
| 6B8N Crystal Structure of the Ca2+/CaM:Kv7.4 (KCNQ4) AB Domain Complex, 10 uM CaCl2 soak Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；2M ammonium sulfate, 0.1M BisTris pH 6.5, 0.01mM CaCl2
|
Resolution 2.20 Å R-free 0.264 |
| 6B8N Crystal Structure of the Ca2+/CaM:Kv7.4 (KCNQ4) AB Domain Complex, 10 uM CaCl2 soak Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；2M ammonium sulfate, 0.1M BisTris pH 6.5, 0.01mM CaCl2
|
Resolution 2.20 Å R-free 0.264 |
| 6B8N Crystal Structure of the Ca2+/CaM:Kv7.4 (KCNQ4) AB Domain Complex, 10 uM CaCl2 soak Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain F
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；2M ammonium sulfate, 0.1M BisTris pH 6.5, 0.01mM CaCl2
|
Resolution 2.20 Å R-free 0.264 |
| 6B8N Crystal Structure of the Ca2+/CaM:Kv7.4 (KCNQ4) AB Domain Complex, 10 uM CaCl2 soak Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain H
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；2M ammonium sulfate, 0.1M BisTris pH 6.5, 0.01mM CaCl2
|
Resolution 2.20 Å R-free 0.264 |
| 6B8P Crystal Structure of the Mg2+/CaM:Kv7.4 (KCNQ4) AB domain complex Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–149（149 aa）
|
Not recorded | MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；2M ammonium sulfate, 0.1M BisTris pH 6.5, 1mM MgCl2
|
Resolution 2.20 Å R-free 0.240 |
| 6B8P Crystal Structure of the Mg2+/CaM:Kv7.4 (KCNQ4) AB domain complex Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–149（149 aa）
|
Not recorded | MG MAGNESIUM ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；2M ammonium sulfate, 0.1M BisTris pH 6.5, 1mM MgCl2
|
Resolution 2.20 Å R-free 0.240 |
| 6B8P Crystal Structure of the Mg2+/CaM:Kv7.4 (KCNQ4) AB domain complex Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain F
1–149（149 aa）
|
Not recorded | MG MAGNESIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；2M ammonium sulfate, 0.1M BisTris pH 6.5, 1mM MgCl2
|
Resolution 2.20 Å R-free 0.240 |
| 6B8P Crystal Structure of the Mg2+/CaM:Kv7.4 (KCNQ4) AB domain complex Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain H
1–149（149 aa）
|
Not recorded | MG MAGNESIUM ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；2M ammonium sulfate, 0.1M BisTris pH 6.5, 1mM MgCl2
|
Resolution 2.20 Å R-free 0.240 |
| 6B8Q Crystal Structure of the Mg2+/CaM:Kv7.5 (KCNQ5) AB domain complex Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–149（149 aa）
|
Not recorded | MG MAGNESIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2M magnesium formate, 20% PEG 3350
|
Resolution 2.60 Å R-free 0.270 |
| 6B8Q Crystal Structure of the Mg2+/CaM:Kv7.5 (KCNQ5) AB domain complex Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–149（149 aa）
|
Not recorded | MG MAGNESIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2M magnesium formate, 20% PEG 3350
|
Resolution 2.60 Å R-free 0.270 |
| 6B8Q Crystal Structure of the Mg2+/CaM:Kv7.5 (KCNQ5) AB domain complex Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain F
1–149（149 aa）
|
Not recorded | MG MAGNESIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2M magnesium formate, 20% PEG 3350
|
Resolution 2.60 Å R-free 0.270 |
| 6B8Q Crystal Structure of the Mg2+/CaM:Kv7.5 (KCNQ5) AB domain complex Deposited 2017-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain H
1–149（149 aa）
|
Not recorded | MG MAGNESIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.2M magnesium formate, 20% PEG 3350
|
Resolution 2.60 Å R-free 0.270 |
| 6BUT Solution structure of full-length apo mammalian calmodulin bound to the IQ motif of the human voltage-gated sodium channel NaV1.2 Deposited 2017-12-11 | Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
2–149（148 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 100；Pressure 1
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 100；Pressure 1
NMR sample composition
0.950 mM U-99% C13, U-99% N15 Calmodulin, 0.950 mM U-99% C13, U-99% N15 voltage-gated sodium channel NaV1.2 IQ motif, 0.1 mM U-98% 2H EDTA, 100 mM KCl, 10 mM [U-99% 2H] imidazole, 0.01 % w/v sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.95 mM U-99% C13, U-99% N15 Calmodulin, 0.95 mM U-99% C13, U-99% N15 voltage-gated sodium channel NaV1.2 IQ motif, 0.1 mM U-98% 2H EDTA, 100 mM potassium chloride, 10 mM [U-99% 2H] imidazole, 0.01 % w/v sodium azide, 100% D2O | 100% D2O
|
Resolution not provided |
| 6CNM Cryo-EM structure of the human SK4/calmodulin channel complex Deposited 2018-03-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | K POTASSIUM ION × 5 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 LMT DODECYL-BETA-D-MALTOSIDE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 6CNN Cryo-EM structure of the human SK4/calmodulin channel complex in the Ca2+ bound state I Deposited 2018-03-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | K POTASSIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 LMT DODECYL-BETA-D-MALTOSIDE × 8 CA CALCIUM ION × 12 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6CNO Cryo-EM structure of the human SK4/calmodulin channel complex in the Ca2+ bound state II Deposited 2018-03-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 12 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.70 Å |
| 6DAD 1.65 Angstrom crystal structure of the N97I Ca/CaM:CaV1.2 IQ domain complex Deposited 2018-05-01 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
2–149（148 aa）
|
Mutation：N97I | CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；2.1 M sodium malonate, 0.1 M HEPES, pH 7.5
|
Resolution 1.65 Å R-free 0.208 |
| 6DAD 1.65 Angstrom crystal structure of the N97I Ca/CaM:CaV1.2 IQ domain complex Deposited 2018-05-01 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
2–149（148 aa）
|
Mutation：N97I | CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；2.1 M sodium malonate, 0.1 M HEPES, pH 7.5
|
Resolution 1.65 Å R-free 0.208 |
| 6DAE 2.0 Angstrom crystal structure of the D95V Ca/CaM:CaV1.2 IQ domain complex Deposited 2018-05-01 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
2–149（148 aa）
|
Mutation：D95V | CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 10.3；277 K；1 M lithium chloride, 30% PEG6000, 0.1M Bicine, pH 10.3
|
Resolution 2.00 Å R-free 0.226 |
| 6DAE 2.0 Angstrom crystal structure of the D95V Ca/CaM:CaV1.2 IQ domain complex Deposited 2018-05-01 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
2–149（148 aa）
|
Mutation：D95V | CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 10.3；277 K；1 M lithium chloride, 30% PEG6000, 0.1M Bicine, pH 10.3
|
Resolution 2.00 Å R-free 0.226 |
| 6DAF 2.4 Angstrom crystal structure of the F141L Ca/CaM:CaV1.2 IQ domain complex Deposited 2018-05-01 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
2–149（148 aa）
|
Mutation：F141L | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.5 M potassium nitrate, 20% PEG3350
|
Resolution 2.40 Å R-free 0.243 |
| 6DAF 2.4 Angstrom crystal structure of the F141L Ca/CaM:CaV1.2 IQ domain complex Deposited 2018-05-01 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
2–149（148 aa）
|
Mutation：F141L | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.5 M potassium nitrate, 20% PEG3350
|
Resolution 2.40 Å R-free 0.243 |
| 6DAH 2.5 Angstrom crystal structure of the N97S CaM mutant Deposited 2018-05-01 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–149（149 aa）
|
Mutation：N97S | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；20% PEG3350, 0.1 M Bis-Tris, pH 6.5
|
Resolution 2.50 Å R-free 0.279 |
| 6DAH 2.5 Angstrom crystal structure of the N97S CaM mutant Deposited 2018-05-01 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–149（149 aa）
|
Mutation：N97S | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；20% PEG3350, 0.1 M Bis-Tris, pH 6.5
|
Resolution 2.50 Å R-free 0.279 |
| 6DAH 2.5 Angstrom crystal structure of the N97S CaM mutant Deposited 2018-05-01 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1–149（149 aa）
|
Mutation：N97S | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；20% PEG3350, 0.1 M Bis-Tris, pH 6.5
|
Resolution 2.50 Å R-free 0.279 |
| 6DAH 2.5 Angstrom crystal structure of the N97S CaM mutant Deposited 2018-05-01 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
1–149（149 aa）
|
Mutation：N97S | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；20% PEG3350, 0.1 M Bis-Tris, pH 6.5
|
Resolution 2.50 Å R-free 0.279 |
| 6E2F Cryo-EM structure of human TRPV6 in complex with Calmodulin Deposited 2018-07-11 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain E
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6E2G Cryo-EM structure of rat TRPV6 in complex with Calmodulin Deposited 2018-07-11 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain E
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6EEB Calmodulin in complex with malbrancheamide Deposited 2018-08-13 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–149（149 aa）
|
Not recorded | J6P (5aS,12aS,13aS)-8,9-dichloro-12,12-dimethyl-2,3,11,12,12a,13-hexahydro-1H,5H,6H-5a,13a-(epiminomethano)indolizino[7,6-b]carbazol-14-one × 1 CA CALCIUM ION × 4 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M Tris pH 8.5, 0.2 M MgCl2, 20% (w/v) PEG 8000
|
Resolution 1.96 Å R-free 0.263 |
| 6EEB Calmodulin in complex with malbrancheamide Deposited 2018-08-13 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–149（149 aa）
|
Not recorded | J6P (5aS,12aS,13aS)-8,9-dichloro-12,12-dimethyl-2,3,11,12,12a,13-hexahydro-1H,5H,6H-5a,13a-(epiminomethano)indolizino[7,6-b]carbazol-14-one × 2 CA CALCIUM ION × 8 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M Tris pH 8.5, 0.2 M MgCl2, 20% (w/v) PEG 8000
|
Resolution 1.96 Å R-free 0.263 |
| 6FEG Solution Structure of CaM/Kv7.2-hAB Complex Deposited 2018-01-02 | Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 2 |
SOLUTION NMR
NMR measurement conditions
pH 6；303 K；Ionic strength (raw mmCIF value) 120；Pressure 1
NMR sample composition
1 mM [U-100% 13C; U-100% 15N; U-50% 2H] Kv7.2-hAB, 1 mM [U-100% 13C; U-100% 15N; U-50% 2H] Calmodulin, 120 mM potassium chloride, 20 mM MES, 2 uM sodium azide, 200 uM [U-2H] DSS, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
500 uM [U-100% 13C; U-100% 15N] Kv7.2-hAB, 500 uM Calmodulin, 120 mM potassium chloride, 20 mM MES, 2 uM sodium azide, 200 uM [U-2H] DSS, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
500 uM Kv7.2-hAB, 500 uM [U-100% 13C; U-100% 15N] Calmodulin, 120 mM potassium chloride, 20 mM MES, 2 uM sodium azide, 200 uM [U-2H] DSS, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
250 uM [U-100% 15N] Kv7.2-hAB, 250 uM [U-100% 15N] Calmodulin, 120 mM potassium chloride, 20 mM MES, 2 uM sodium azide, 200 uM [U-2H] DSS, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 6FEH Solution Structure of CaM/Kv7.2-hAB Complex Deposited 2018-01-02 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 4 |
SOLUTION NMR
NMR measurement conditions
pH 6；303 K；Ionic strength (raw mmCIF value) 120；Pressure 1
NMR sample composition
1 mM [U-100% 13C; U-100% 15N; U-50% 2H] Kv7.2-hAB, 1 mM [U-100% 13C; U-100% 15N; U-50% 2H] Calmodulin, 120 mM potassium chloride, 20 mM MES, 5 mM CaCl2, 2 uM sodium azide, 200 uM [U-2H] DSS, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
500 uM [U-100% 13C; U-100% 15N] Kv7.2-hAB, 500 uM Calmodulin, 120 mM potassium chloride, 20 mM MES, 5 mM CaCl2, 2 uM sodium azide, 200 uM [U-2H] DSS, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
250 uM [U-100% 15N] Kv7.2-hAB, 250 uM [U-100% 15N] Calmodulin, 120 mM potassium chloride, 20 mM MES, 5 mM CaCl2, 2 uM sodium azide, 200 uM [U-2H] DSS, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 6GDK Calcium bound form of human calmodulin mutant F141L Deposited 2018-04-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–149（148 aa）
|
Mutation：F141L | CA CALCIUM ION × 4 |
SOLUTION NMR
NMR measurement conditions
pH 6.45；298.1 K；Ionic strength (raw mmCIF value) 24；Pressure 1
NMR sample composition
0.53 mM [U-13C; U-15N] Calmodulin mutant F141L, 10 mM NA calcium chloride, 10 mM NA potassium chloride, 2 mM NA HEPES, 2 mM NA sodium azide, 0.1 mM NA TSP-d4, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 6GDL Calmodulin mutant - F141L apo-form Unstructured C-domain Deposited 2018-04-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–149（148 aa）
|
Mutation：F141L | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6.55；298.1 K；Ionic strength (raw mmCIF value) 24；Pressure 1
NMR sample composition
580 uM [U-99% 13C; U-99% 15N] Calmodulin, 2 mM HEPES, 10 mM potassium chloride, 10 mM EDTA, 2 mM sodium azide, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 6HCS Crystal structure of CaM-peptide complex containing AzF at position 108 Deposited 2018-08-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–149（149 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；PEG 6000, lithium chloride, sodium chloride
|
Resolution 2.00 Å R-free 0.294 |
| 6HCS Crystal structure of CaM-peptide complex containing AzF at position 108 Deposited 2018-08-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–149（149 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；PEG 6000, lithium chloride, sodium chloride
|
Resolution 2.00 Å R-free 0.294 |
| 6HCS Crystal structure of CaM-peptide complex containing AzF at position 108 Deposited 2018-08-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
1–149（149 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；PEG 6000, lithium chloride, sodium chloride
|
Resolution 2.00 Å R-free 0.294 |
| 6HCS Crystal structure of CaM-peptide complex containing AzF at position 108 Deposited 2018-08-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain G
1–149（149 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；PEG 6000, lithium chloride, sodium chloride
|
Resolution 2.00 Å R-free 0.294 |
| 6HR1 Crystal structure of the YFPnano fusion protein Deposited 2018-09-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–149（148 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | TLA L(+)-TARTARIC ACID × 1 CA CALCIUM ION × 4 EDO 1,2-ETHANEDIOL × 2 GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.8；293 K；24% w/v PEG 3350
0.2 M di-Ammonium tartrate
10% v/v Glycerol
|
Resolution 1.90 Å R-free 0.214 |
| 6HR1 Crystal structure of the YFPnano fusion protein Deposited 2018-09-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
2–149（148 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 4 EDO 1,2-ETHANEDIOL × 7 GOL GLYCEROL × 2 NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.8；293 K；24% w/v PEG 3350
0.2 M di-Ammonium tartrate
10% v/v Glycerol
|
Resolution 1.90 Å R-free 0.214 |
| 6JI8 Structure of RyR2 (F/apoCaM dataset) Deposited 2019-02-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain C
1–149（149 aa）
Chain F
1–149（149 aa）
Chain I
1–149（149 aa）
Chain L
1–149（149 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6JII Structure of RyR2 (F/A/C/L-Ca2+/apo-CaM-M dataset) Deposited 2019-02-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain C
1–149（149 aa）
Chain F
1–149（149 aa）
Chain I
1–149（149 aa）
Chain L
1–149（149 aa）
|
Mutation：E32A,E68A,E105A,E141A Mutation：E32A,E68A,E105A,E141A Mutation：E32A,E68A,E105A,E141A Mutation：E32A,E68A,E105A,E141A | ZN ZINC ION × 4 CA CALCIUM ION × 4 CFF CAFFEINE × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6JIU Structure of RyR2 (F/A/C/L-Ca2+/Ca2+CaM dataset) Deposited 2019-02-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain C
1–149（149 aa）
Chain F
1–149（149 aa）
Chain I
1–149（149 aa）
Chain L
1–149（149 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 12 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6JIY Structure of RyR2 (F/A/C/H-Ca2+/Ca2+CaM dataset) Deposited 2019-02-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain C
1–149（149 aa）
Chain F
1–149（149 aa）
Chain I
1–149（149 aa）
Chain L
1–149（149 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 20 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6JRS Structure of RyR2 (*F/A/C/L-Ca2+/Ca2+-CaM dataset) Deposited 2019-04-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain C
1–149（149 aa）
Chain F
1–149（149 aa）
Chain I
1–149（149 aa）
Chain L
1–149（149 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 12 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6JV2 Structure of RyR2 (P/L-Ca2+/Ca2+-CaM dataset) Deposited 2019-04-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–149（149 aa）
Chain D
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 20 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å |
| 6K4K Crystal structure of SidJ-CaM binary complex at 2.71 A Deposited 2019-05-24 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；299 K；20% PEG 3350, 0.2M NaI
|
Resolution 2.71 Å R-free 0.243 |
| 6K4K Crystal structure of SidJ-CaM binary complex at 2.71 A Deposited 2019-05-24 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；299 K；20% PEG 3350, 0.2M NaI
|
Resolution 2.71 Å R-free 0.243 |
| 6K4L Crystal structure of Se-labelled SidJ complex with CaM at 2.95 A Deposited 2019-05-24 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–149（149 aa）
|
Not recorded | CL CHLORIDE ION × 2 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；299 K；25% PEG 3350, 0.2M NaI
|
Resolution 2.95 Å R-free 0.269 |
| 6K4L Crystal structure of Se-labelled SidJ complex with CaM at 2.95 A Deposited 2019-05-24 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–149（149 aa）
|
Not recorded | CL CHLORIDE ION × 2 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；299 K；25% PEG 3350, 0.2M NaI
|
Resolution 2.95 Å R-free 0.269 |
| 6K4R Crystal structure of SidJ-CaM-AMP ternary complex at 3.11 A Deposited 2019-05-26 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–149（149 aa）
|
Not recorded | AMP ADENOSINE MONOPHOSPHATE × 1 CL CHLORIDE ION × 2 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；299 K；25% PEG 3350, 0.2M NaI, 0.1M HEPES 7.5
|
Resolution 3.11 Å R-free 0.279 |
| 6K4R Crystal structure of SidJ-CaM-AMP ternary complex at 3.11 A Deposited 2019-05-26 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–149（149 aa）
|
Not recorded | AMP ADENOSINE MONOPHOSPHATE × 1 CL CHLORIDE ION × 2 PG4 TETRAETHYLENE GLYCOL × 2 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；299 K；25% PEG 3350, 0.2M NaI, 0.1M HEPES 7.5
|
Resolution 3.11 Å R-free 0.279 |
| 6M2W Structure of RyR1 (Ca2+/Caffeine/ATP/CaM1234/CHL) Deposited 2020-03-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain C
1–149（149 aa）
Chain F
1–149（149 aa）
Chain I
1–149（149 aa）
Chain L
1–149（149 aa）
|
Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A | CFF CAFFEINE × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 F0U 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6M7H Structure of calmodulin with KN93 Deposited 2018-08-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–148（147 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | KN9 N-[2-[[[3-(4'-Chlorophenyl)-2-propenyl]methylamino]methyl]phenyl]-N-(2-hydroxyethyl)-4'-methoxybenzenesulfonamide × 3 CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；289 K；bis tris ph 6.5, peg 3350
|
Resolution 1.60 Å R-free 0.267 |
| 6MUD Voltage-gated sodium channel NaV1.5 C-terminal domain in complex with Ca2+/Calmodulin Deposited 2018-10-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9.5；277 K；5-15 % (w/v) PEG 4000, 0.1 M Tris, pH 9.5, 0.1 M MgCl2, and 5 % (v/v) isopropanol
|
Resolution 2.69 Å R-free 0.269 |
| 6MUE Voltage-gated sodium channel NaV1.4 IQ domain in complex with Ca2+/Calmodulin Deposited 2018-10-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；0.1 M MES pH 6.0 and 55 % (v/v) isopropanol
|
Resolution 1.90 Å R-free 0.260 |
| 6N5W Crystal structure of the Ca2+/CaM complex with independent peptides of Kv7.4 (KCNQ4) A & B domains Deposited 2018-11-22 | Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；295 K；1.3 M sodium citrate, 0.1 M HEPES pH 7.0
|
Resolution 2.15 Å R-free 0.274 |
| 6O5G Calmodulin in complex with isomalbrancheamide D Deposited 2019-03-02 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–149（149 aa）
|
Not recorded | LMJ (5aS,12aS,13aS)-9-bromo-8-chloro-12,12-dimethyl-2,3,11,12,12a,13-hexahydro-1H,5H,6H-5a,13a-(epiminomethano)indolizino[7 ,6-b]carbazol-14-one × 1 CA CALCIUM ION × 4 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.1 M Tris pH 8.5, 0.2 M MgCl2, 20% PEG 8000
|
Resolution 1.89 Å R-free 0.226 |
| 6OS4 Calmodulin in complex with farnesyl cysteine methyl ester Deposited 2019-05-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–149（148 aa）
|
Not recorded | CA CALCIUM ION × 4 5U0 s-farnesyl-l-cysteine methyl ester × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；0.2mM sodium acetate, 0.1mM cacodylate, 28% PEG 8000
|
Resolution 2.05 Å R-free 0.225 |
| 6OS4 Calmodulin in complex with farnesyl cysteine methyl ester Deposited 2019-05-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–149（148 aa）
|
Not recorded | CA CALCIUM ION × 8 5U0 s-farnesyl-l-cysteine methyl ester × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.7；293 K；0.2mM sodium acetate, 0.1mM cacodylate, 28% PEG 8000
|
Resolution 2.05 Å R-free 0.225 |
| 6PAW Crystal structure of DAPK2 S308A Calcium/Calmodulin complex Deposited 2019-06-12 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；292.15 K；0.1 M Bis-Tris-HCl, pH 6.5, 0.6 M sodium chloride, 14.3% PEG3350
|
Resolution 2.95 Å R-free 0.288 |
| 6PAW Crystal structure of DAPK2 S308A Calcium/Calmodulin complex Deposited 2019-06-12 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；292.15 K；0.1 M Bis-Tris-HCl, pH 6.5, 0.6 M sodium chloride, 14.3% PEG3350
|
Resolution 2.95 Å R-free 0.288 |
| 6PAW Crystal structure of DAPK2 S308A Calcium/Calmodulin complex Deposited 2019-06-12 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain H
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；292.15 K；0.1 M Bis-Tris-HCl, pH 6.5, 0.6 M sodium chloride, 14.3% PEG3350
|
Resolution 2.95 Å R-free 0.288 |
| 6PAW Crystal structure of DAPK2 S308A Calcium/Calmodulin complex Deposited 2019-06-12 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain G
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；292.15 K；0.1 M Bis-Tris-HCl, pH 6.5, 0.6 M sodium chloride, 14.3% PEG3350
|
Resolution 2.95 Å R-free 0.288 |
| 6PBX Single particle cryo-EM structure of the voltage-gated K+ channel Eag1 3-13 deletion mutant bound to calmodulin (conformation 2) Deposited 2019-06-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–149（149 aa）
Chain D
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 6PBY Single particle cryo-EM structure of the voltage-gated K+ channel Eag1 3-13 deletion mutant bound to calmodulin (conformation 1) Deposited 2019-06-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–149（149 aa）
Chain D
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.67 Å |
| 6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
2–149（148 aa）
|
Mutation：D129G | CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
|
Resolution 2.40 Å R-free 0.283 |
| 6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 10 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain S
2–149（148 aa）
|
Mutation：D129G | CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
|
Resolution 2.40 Å R-free 0.283 |
| 6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
2–149（148 aa）
|
Mutation：D129G | CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
|
Resolution 2.40 Å R-free 0.283 |
| 6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
2–149（148 aa）
|
Mutation：D129G | CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
|
Resolution 2.40 Å R-free 0.283 |
| 6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain G
2–149（148 aa）
|
Mutation：D129G | CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
|
Resolution 2.40 Å R-free 0.283 |
| 6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain I
2–149（148 aa）
|
Mutation：D129G | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
|
Resolution 2.40 Å R-free 0.283 |
| 6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain K
2–149（148 aa）
|
Mutation：D129G | CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
|
Resolution 2.40 Å R-free 0.283 |
| 6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 7 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain M
2–149（148 aa）
|
Mutation：D129G | CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
|
Resolution 2.40 Å R-free 0.283 |
| 6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 8 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain O
2–149（148 aa）
|
Mutation：D129G | CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
|
Resolution 2.40 Å R-free 0.283 |
| 6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 9 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain Q
2–149（148 aa）
|
Mutation：D129G | CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
|
Resolution 2.40 Å R-free 0.283 |
| 6U3A 1.65 Angstrom crystal structure of the N97S Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
2–149（148 aa）
|
Mutation：N97S | CA CALCIUM ION × 3 NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；DL-malic acid
|
Resolution 1.65 Å R-free 0.209 |
| 6U3A 1.65 Angstrom crystal structure of the N97S Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
2–149（148 aa）
|
Mutation：N97S | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；DL-malic acid
|
Resolution 1.65 Å R-free 0.209 |
| 6U3B 1.7 Angstrom crystal structure of the Q135P Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
2–149（148 aa）
|
Mutation：Q135P | CA CALCIUM ION × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；PEG 550 MME, sodium acetate
|
Resolution 1.70 Å R-free 0.200 |
| 6U3D 1.75 Angstrom crystal structure of the N53I Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
2–149（148 aa）
|
Mutation：N53I | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；PEG 3350, citric acid
|
Resolution 1.75 Å R-free 0.201 |
| 6U3D 1.75 Angstrom crystal structure of the N53I Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
2–149（148 aa）
|
Mutation：N53I | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；PEG 3350, citric acid
|
Resolution 1.75 Å R-free 0.201 |
| 6UZZ structure of human KCNQ1-CaM complex Deposited 2019-11-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–149（149 aa）
Chain D
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6V00 structure of human KCNQ1-KCNE3-CaM complex Deposited 2019-11-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain B
1–149（149 aa）
Chain E
1–149（149 aa）
Chain H
1–149（149 aa）
Chain K
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6V01 structure of human KCNQ1-KCNE3-CaM complex with PIP2 Deposited 2019-11-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain B
1–149（149 aa）
Chain E
1–149（149 aa）
Chain H
1–149（149 aa）
Chain K
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 8 PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6X32 Wt pig RyR1 in complex with apoCaM, EGTA condition (class 1 and 2, closed) Deposited 2020-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain C
3–148（146 aa）
Chain F
3–148（146 aa）
Chain I
3–148（146 aa）
Chain L
3–148（146 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6X33 Wt pig RyR1 in complex with apoCaM, EGTA condition (class 3, open) Deposited 2020-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain C
1–148（148 aa）
Chain F
1–148（148 aa）
Chain I
1–148（148 aa）
Chain L
1–148（148 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6X35 Pig R615C RyR1 in complex with CaM, EGTA (class 1, open) Deposited 2020-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain C
1–148（148 aa）
Chain F
1–148（148 aa）
Chain I
1–148（148 aa）
Chain L
1–148（148 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6X36 Pig R615C RyR1 in complex with CaM, EGTA (class 3, closed) Deposited 2020-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain C
1–148（148 aa）
Chain F
1–148（148 aa）
Chain I
1–148（148 aa）
Chain L
1–148（148 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.70 Å |
| 6XXX 1.25 Angstrom crystal structure of Ca/CaM A102V:RyR2 peptide complex Deposited 2020-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain AAA
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M Sodium acetate trihydrate pH 4.0, 25% w/v Polyethylene glycol 1,500
|
Resolution 1.25 Å R-free 0.189 |
| 6XY3 2.0 Angstrom crystal structure of Ca/CaM N53I:RyR2 peptide complex Deposited 2020-01-29 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain AAA
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M Sodium acetate trihydrate pH 4.5, 10% w/v Polyethylene glycol 10,000
|
Resolution 2.00 Å R-free 0.260 |
| 6XYR Structure of the T4Lnano fusion protein Deposited 2020-01-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–149（148 aa）
|
Not recorded | CA CALCIUM ION × 5 GOL GLYCEROL × 5 CL CHLORIDE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；8% PEG 8000, 200 mM LiCl2, 100 mM Tris pH 8.0, 15% Glycerol
|
Resolution 2.08 Å R-free 0.236 |
| 6Y4P Calmodulin N53I variant bound to cardiac ryanodine receptor (RyR2) calmodulin binding domain Deposited 2020-02-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–149（149 aa）
|
Mutation：N53I | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298.15 K；0.1 M Sodium Acetate pH 4.70 and 23 % PEG 550 MME
|
Resolution 2.13 Å R-free 0.246 |
| 6Y94 Ca2+-bound Calmodulin mutant N53I Deposited 2020-03-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–149（148 aa）
|
Mutation：N53I | CA CALCIUM ION × 4 |
SOLUTION NMR
NMR measurement conditions
pH 6.57；298.1 K；Ionic strength (raw mmCIF value) 182；Pressure 1
NMR measurement conditions
pH 6.57；298.1 K；Ionic strength (raw mmCIF value) 42；Pressure 1
NMR measurement conditions
pH 6.57；298.1 K；Ionic strength (raw mmCIF value) 42；Pressure 1
NMR sample composition
0.56 mM [U-99% 13C; U-99% 15N] Calmodulin N53I, 2 mM HEPES, 100 mM potassium chloride, 10 mM calcium chloride, 50 mM sodium acetate, 2 mM sodium azide, 0.05 mM TSP, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
1.0 mM [U-99% 15N] Calmodulin N53I, 2 mM HEPES, 10 mM potassium chloride, 10 mM calcium chloride, 2 mM sodium azide, 0.05 mM TSP, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
1.0 mM [U-99% 13C; U-99% 15N] Calmodulin, 2 mM HEPES, 10 mM potassium chloride, 10 mM calcium chloride, 2 mM sodium azide, 0.05 mM TSP, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 6Y95 Ca2+-free Calmodulin mutant N53I Deposited 2020-03-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–149（148 aa）
|
Mutation：N53I | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6.3；298.1 K；Ionic strength (raw mmCIF value) 105；Pressure 1
NMR measurement conditions
pH 6.56；298.1 K；Ionic strength (raw mmCIF value) 42；Pressure 1
NMR measurement conditions
pH 6.53；298.1 K；Ionic strength (raw mmCIF value) 42；Pressure 1
NMR sample composition
1.9 mM [U-99% 13C; U-99% 15N] Calmodulin N53I, 20 mM HEPES, 100 mM potassium chloride, 1 mM EDTA, 2 mM sodium azide, 0.05 mM TSP, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
1.2 mM [U-99% 15N] Calmodulin N53I, 2 mM HEPES, 10 mM potassium chloride, 10 mM EDTA, 2 mM sodium azide, 0.05 mM TSP, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
1.2 mM [U-99% 13C; U-99% 15N] Calmodulin, 2 mM HEPES, 10 mM potassium chloride, 10 mM EDTA, 2 mM sodium azide, 0.05 mM TSP, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 6YNS CaM-P458 complex (crystal form 2) Deposited 2020-04-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
2–149（148 aa）
Chain C
2–149（148 aa）
|
Not recorded | CA CALCIUM ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；300 K；0.2M of MgCl2
0.1M HEPES-HCl pH 7.5
25% (w/v) PEG3350
|
Resolution 3.94 Å R-free 0.284 |
| 6YNS CaM-P458 complex (crystal form 2) Deposited 2020-04-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
2–149（148 aa）
Chain K
2–149（148 aa）
|
Not recorded | CA CALCIUM ION × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；300 K；0.2M of MgCl2
0.1M HEPES-HCl pH 7.5
25% (w/v) PEG3350
|
Resolution 3.94 Å R-free 0.284 |
| 6YNS CaM-P458 complex (crystal form 2) Deposited 2020-04-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain D
2–149（148 aa）
Chain E
2–149（148 aa）
|
Not recorded | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；300 K；0.2M of MgCl2
0.1M HEPES-HCl pH 7.5
25% (w/v) PEG3350
|
Resolution 3.94 Å R-free 0.284 |
| 6YNS CaM-P458 complex (crystal form 2) Deposited 2020-04-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain F
2–149（148 aa）
Chain G
2–149（148 aa）
|
Not recorded | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；300 K；0.2M of MgCl2
0.1M HEPES-HCl pH 7.5
25% (w/v) PEG3350
|
Resolution 3.94 Å R-free 0.284 |
| 6YNS CaM-P458 complex (crystal form 2) Deposited 2020-04-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain H
2–149（148 aa）
Chain I
2–149（148 aa）
|
Not recorded | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；300 K；0.2M of MgCl2
0.1M HEPES-HCl pH 7.5
25% (w/v) PEG3350
|
Resolution 3.94 Å R-free 0.284 |
| 6YNS CaM-P458 complex (crystal form 2) Deposited 2020-04-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain J
2–149（148 aa）
Chain L
2–149（148 aa）
|
Not recorded | CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；300 K；0.2M of MgCl2
0.1M HEPES-HCl pH 7.5
25% (w/v) PEG3350
|
Resolution 3.94 Å R-free 0.284 |
| 6YNU CaM-P458 complex (crystal form 1) Deposited 2020-04-14 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
2–149（148 aa）
|
Not recorded | CA CALCIUM ION × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；300 K；0.3 M Ammonium sulfate
30% (w/v) PEG 4000
|
Resolution 3.12 Å R-free 0.291 |
| 6YNU CaM-P458 complex (crystal form 1) Deposited 2020-04-14 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
2–149（148 aa）
|
Not recorded | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；300 K；0.3 M Ammonium sulfate
30% (w/v) PEG 4000
|
Resolution 3.12 Å R-free 0.291 |
| 6ZBI Ternary complex of Calmodulin bound to 2 molecules of NHE1 Deposited 2020-06-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
2–149（148 aa）
|
Not recorded | CA CALCIUM ION × 4 |
SOLUTION NMR
NMR measurement conditions
pH 7.5；310 K；Ionic strength (raw mmCIF value) 120；Pressure 1
NMR sample composition
0.5 mM [U-99% 13C; U-99% 15N] Calmodulin, 1.15 mM Sodium/Hydrogen exchanger 1 (NHE1, SLC9A1), 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
1 mM [U-99% 13C; U-99% 15N] Sodium/Hydrogen exchanger 1 (NHE1, SLC9A1), 0.5 mM Calmodulin, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 7BF1 Ca2+-Calmodulin in complex with peptide from brain-type creatine kinase in extended 1:2 binding mode Deposited 2020-12-31 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain AAA
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 4 ACE ACETYL GROUP × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；25% PEG 1500, 0.1 M SPG buffer pH 4-5.
CaM-CKBpeptide ratios of 1:4
|
Resolution 1.24 Å R-free 0.186 |
| 7BF2 Ca2+-Calmodulin in complex with human muscle form creatine kinase peptide in extended 1:2 binding mode Deposited 2020-12-31 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain AAA
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；25% PEG 1500, 0.1 M SPG buffer pH 4-5.
CaM-CKBpeptide ratios of 1:4
|
Resolution 1.43 Å R-free 0.228 |
| 7KL5 Structure of Calmodulin Bound to the Cardiac Ryanodine Receptor (RyR2) at Residues: Phe4246 to Val4271 Deposited 2020-10-29 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 5 EDO 1,2-ETHANEDIOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；277 K；0.2M sodium acetate, 0.1M Tris pH 8.5, 30%(w/v) PEG 4000
|
Resolution 1.65 Å R-free 0.245 |
| 7L8V NMR Structure of half-calcified calmodulin mutant (CaMEF12) bound to the IQ-motif of CaV1.2 Deposited 2021-01-01 | Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–149（149 aa）
|
Mutation：D21A, D23A, D25A, E32Q, D57A, D59A, N61A, E68Q | CA CALCIUM ION × 2 |
SOLUTION NMR
NMR measurement conditions
pH 7.4；303 K；Ionic strength (raw mmCIF value) 0.1；Pressure 1
NMR sample composition
0.5 mM [U-95% 15N] calmodulin, 0.75 mM IQ-motif, 10 mM TRIS, 2 mM CALCIUM ION, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5 mM [U-13C; U-15N] calmodulin, 0.75 mM IQ-motif, 10 mM TRIS, 2 mM CALCIUM ION, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 7PSZ Crystal structure of CaM in complex with CDZ (form 1) Deposited 2021-09-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–149（148 aa）
|
Not recorded | 85H 1-[bis(4-chlorophenyl)methyl]-3-[(2~{R})-2-(2,4-dichlorophenyl)-2-[(2,4-dichlorophenyl)methoxy]ethyl]imidazole × 1 SO4 SULFATE ION × 1 CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；292 K；30 %w/v PEG 8K and 0.2 M (NH4)2SO4
|
Resolution 1.90 Å R-free 0.251 |
| 7PU9 Crystal structure of CaM in complex with CDZ (form 2) Deposited 2021-09-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–149（148 aa）
|
Not recorded | 85H 1-[bis(4-chlorophenyl)methyl]-3-[(2~{R})-2-(2,4-dichlorophenyl)-2-[(2,4-dichlorophenyl)methoxy]ethyl]imidazole × 2 CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；292 K；0.2 M CaCl2, 0.1 M Tris pH 8.5 and 25 %w/v PEG 4K
|
Resolution 2.28 Å R-free 0.266 |
| 7SHQ Structure of a functional construct of eukaryotic elongation factor 2 kinase in complex with calmodulin. Deposited 2021-10-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
2–149（148 aa）
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 5 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 6.9；298.15 K；Cocktail solution:
Peg3350: 24%
MgCl2: 300 mM
BisTris pH 6.9: 100 mM
Protein Solution:
11 mg/mL CaM-eEF2Kp1/1
Tris pH 7.5: 20 mM
NaCl: 0.1 M
CaCl2: 3mM
TCEP: 1mM
MgCl2: 1.5 mM
AMPPNP: 1.0 mM
Crystallization conditions: 1/1 Protein/Cocktail under Paraffin Oil in a Greiner 72-Well microbatch plate
|
Resolution 2.34 Å R-free 0.252 |
| 7SX3 Human NALCN-FAM155A-UNC79-UNC80 channelosome with CaM bound, conformation 1/2 Deposited 2021-11-22 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric |
Chain C
1–149（149 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 PEV (1S)-2-{[(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE × 4 PGV (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE × 2 Y01 CHOLESTEROL HEMISUCCINATE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 sec blotting
|
Resolution 3.10 Å |
| 7SX4 Human NALCN-FAM155A-UNC79-UNC80 channelosome with CaM bound, conformation 2/2 Deposited 2021-11-22 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric |
Chain C
1–149（149 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 PEV (1S)-2-{[(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE × 1 PGV (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE × 1 Y01 CHOLESTEROL HEMISUCCINATE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 sec blotting
|
Resolution 3.50 Å |
| 7T2Q PEGylated Calmodulin-1 (K148U) Deposited 2021-12-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–149（148 aa）
|
Not recorded | CA CALCIUM ION × 4 MG MAGNESIUM ION × 2 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.2 M Magnesium chloride hexahydrate, 0.1 M Tris pH 8.5, 20% w/v PEG 8000
|
Resolution 1.95 Å R-free 0.258 |
| 7T2Q PEGylated Calmodulin-1 (K148U) Deposited 2021-12-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–149（148 aa）
|
Not recorded | CA CALCIUM ION × 8 MG MAGNESIUM ION × 4 ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.2 M Magnesium chloride hexahydrate, 0.1 M Tris pH 8.5, 20% w/v PEG 8000
|
Resolution 1.95 Å R-free 0.258 |
| 7TCI Structure of Xenopus KCNQ1-CaM in complex with ML277 Deposited 2021-12-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–149（149 aa）
Chain D
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | I0S (2R)-N-[4-(4-methoxyphenyl)-1,3-thiazol-2-yl]-1-(4-methylbenzene-1-sulfonyl)piperidine-2-carboxamide × 4 CA CALCIUM ION × 12 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 7TCP Structure of Xenopus KCNQ1-CaM Deposited 2021-12-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–149（149 aa）
Chain D
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 12 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.84 Å |
| 7TZC A drug and ATP binding site in type 1 ryanodine receptor Deposited 2022-02-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain C
1–149（149 aa）
Chain D
1–149（149 aa）
Chain E
1–149（149 aa）
Chain K
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 20 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ZN ZINC ION × 4 CFF CAFFEINE × 4 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 L9R (2S)-3-(octadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.45 Å |
| 7U9T Structure of PKA phosphorylated human RyR2 in the closed state in the presence of Calmodulin Deposited 2022-03-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain I
1–149（149 aa）
Chain J
1–149（149 aa）
Chain K
1–149（149 aa）
Chain L
1–149（149 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.68 Å |
| 7UA3 Structure of PKA phosphorylated human RyR2-R2474S in the closed state in the presence of Calmodulin Deposited 2022-03-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain I
1–149（149 aa）
Chain J
1–149（149 aa）
Chain K
1–149（149 aa）
Chain L
1–149（149 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.97 Å |
| 7UA4 Structure of PKA phosphorylated human RyR2-R2474S in the open state in the presence of Calmodulin Deposited 2022-03-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain I
1–149（149 aa）
Chain J
1–149（149 aa）
Chain K
1–149（149 aa）
Chain L
1–149（149 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 XAN XANTHINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.93 Å |
| 7VMB Crystal structure of IQSEC1-IQ motif, Sec7PH tandem in complex with calmodulin Deposited 2021-10-08 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
1–149（149 aa）
|
Not recorded | GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；289.15 K；0.1M HEPES pH 7.5, 4% w/v Polyethylene glycol 8000
|
Resolution 2.00 Å R-free 0.252 |
| 7VUO Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation F141L Deposited 2021-11-03 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
2–149（148 aa）
|
Mutation：F141L | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；0.1M Tris8.5
25% w/v Polyethylene glycol 3,350
0.2M Trimethylamine N-oxide dihydrate
|
Resolution 2.68 Å R-free 0.274 |
| 7VVD Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation Q135P Deposited 2021-11-05 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–149（149 aa）
|
Mutation：Q135P | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；291 K；25% PEG 1500, 0.1M MMT pH 8.0
|
Resolution 3.13 Å R-free 0.274 |
| 7VVD Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation Q135P Deposited 2021-11-05 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
1–149（149 aa）
|
Mutation：Q135P | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；291 K；25% PEG 1500, 0.1M MMT pH 8.0
|
Resolution 3.13 Å R-free 0.274 |
| 7VVH Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation E140G Deposited 2021-11-06 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–149（149 aa）
Fragment：E141G
|
Not recorded | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；291 K；25% PEG 1500, 0.1M MMT pH 9.0
|
Resolution 2.30 Å R-free 0.272 |
| 7WJI Architecture of the human NALCN channelosome Deposited 2022-01-06 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric |
Chain E
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 7WR3 Crystal structure of MBP-fused OspC3 in complex with calmodulin Deposited 2022-01-26 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–149（149 aa）
|
Not recorded | SO4 SULFATE ION × 2 NCA NICOTINAMIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20% PEG3350, 0.1 M Tris pH 8.2-8.4, 0.2 M Lithium Sulfate, 0.7% 1-Butanol
|
Resolution 1.87 Å R-free 0.228 |
| 7WR3 Crystal structure of MBP-fused OspC3 in complex with calmodulin Deposited 2022-01-26 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
1–149（149 aa）
|
Not recorded | SO4 SULFATE ION × 2 NCA NICOTINAMIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20% PEG3350, 0.1 M Tris pH 8.2-8.4, 0.2 M Lithium Sulfate, 0.7% 1-Butanol
|
Resolution 1.87 Å R-free 0.228 |
| 7WR4 Crystal structure of OspC3-calmodulin-caspase-4 complex Deposited 2022-01-26 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain B
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；25% PEG3350, 0.1 M HEPES pH 7.4, 0.2 M Ammonium sulfate, 0.3 M NDSB-195
|
Resolution 2.75 Å R-free 0.270 |
| 7WR5 Crystal structure of OspC3-calmodulin-caspase-4 complex binding with 2'-aF-NAD+ Deposited 2022-01-26 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain B
1–149（149 aa）
|
Not recorded | 5ZV [[(2~{R},3~{R},4~{S},5~{R})-5-(3-aminocarbonylpyridin-1-yl)-4-fluoranyl-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20% PEG3350, 0.2 M Potassium acetate
|
Resolution 3.10 Å R-free 0.272 |
| 7WZS Crystal structure of Chromobacterium violaceum effector CopC in complex with host calmodulin and caspase-7 Deposited 2022-02-19 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；100 mM Bis-Tris propane pH 7.5, 1.5 M lithium sulfate
|
Resolution 3.60 Å R-free 0.314 |
| 7XN4 Cryo-EM structure of CopC-CaM-caspase-3 with NAD+ Deposited 2022-04-28 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain D
1–149（149 aa）
|
Not recorded | NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.35 Å |
| 7XN5 Cryo-EM structure of CopC-CaM-caspase-3 with ADPR Deposited 2022-04-28 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain D
1–149（149 aa）
|
Not recorded | NCA NICOTINAMIDE × 1 APR ADENOSINE-5-DIPHOSPHORIBOSE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.18 Å |
| 7XN6 Cryo-EM structure of CopC-CaM-caspase-3 with ADPR-deacylization Deposited 2022-04-28 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain D
1–149（149 aa）
|
Not recorded | NCA NICOTINAMIDE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.45 Å |
| 7ZRP 2.65 Angstrom crystal structure of Ca/CaM:CaMKIIdelta peptide complex Deposited 2022-05-04 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
2–149（148 aa）
|
Not recorded | CA CALCIUM ION × 4 PG4 TETRAETHYLENE GLYCOL × 2 IMD IMIDAZOLE × 1 ZN ZINC ION × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；292 K；200 mM Zinc acetate, 100 mM Imidazole pH8.0, 18% PEG3000
|
Resolution 2.65 Å R-free 0.272 |
| 7ZRP 2.65 Angstrom crystal structure of Ca/CaM:CaMKIIdelta peptide complex Deposited 2022-05-04 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
2–149（148 aa）
|
Not recorded | CA CALCIUM ION × 4 IMD IMIDAZOLE × 1 ZN ZINC ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；292 K；200 mM Zinc acetate, 100 mM Imidazole pH8.0, 18% PEG3000
|
Resolution 2.65 Å R-free 0.272 |
| 7ZRQ 1.68 Angstrom crystal structure of Ca/CaM-E140G:CaMKIIdelta peptide complex Deposited 2022-05-04 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
2–149（148 aa）
|
Mutation：E140G | CA CALCIUM ION × 4 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；0.1 M Na+-HEPES, 0.1 M MOPS (acid), pH 7.5, 0.03 M magnesium chloride hexahydrate, 0.03 M calcium chloride dihydrate, 12.5% v/v MPD; 12.5% PEG 1000; 12.5% w/v PEG 3350
|
Resolution 1.68 Å R-free 0.244 |
| 8AHS Crystal structure of human Ca2+/Calmodulin in complex with melittin Deposited 2022-07-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；293 K；0.2M NaCl, 22w/v% PEG3350, 0.1M Bis-Tris, pH 5.4
|
Resolution 2.48 Å R-free 0.270 |
| 8B6Q X-ray structure of the haloalkane dehalogenase HaloTag7 with an insertion of Calmodulin-M13 fusion at position 154-156 that mimic the structure of CaProLa, an calcium gated protein labeling technology Deposited 2022-09-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–148（146 aa）
|
Not recorded | CL CHLORIDE ION × 1 CA CALCIUM ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；0.1 M MES pH 6.0, 0.2 M calcium acetate, 18% (m/v) PEG 8000
|
Resolution 2.60 Å R-free 0.318 |
| 8BFG Solution structure of human apo/Calmodulin G113R (G114R) Deposited 2022-10-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–149（148 aa）
|
Mutation：G113R | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6.64；298.1 K；Ionic strength (raw mmCIF value) 128；Pressure 1
NMR sample composition
1.2 mM aa 81-148: U-13C,15N Calmodulin G113R, 5 mM EDTA, 100 mM potassium chloride, 2 mM NaN3, 20 mM HEPES, 0.1 mM TSP-d4, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 8DGH NMR Structure of calmodulin bound to C-terminal site in the beta-subunit of cyclic nucleotide-gated channel Deposited 2022-06-23 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；308 K；Ionic strength (raw mmCIF value) 1；Pressure 1
NMR sample composition
0.4 mM [U-100% 13C; U-100% 15N] Calmodulin, 1.2 mM Cyclic nucleotide-gated cation channel beta-1, 20 mM [U-100% 2H] TRIS, 1 mM Calcium chloride, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.4 mM [U-100% 13C; U-100% 15N] Calmodulin, 1.2 mM Cyclic nucleotide-gated cation channel beta-1, 20 mM [U-100% 2H] TRIS, 1 mM Calcium chloride, 100% D2O | 100% D2O
|
Resolution not provided |
| 8DGK NMR structure of calmodulin bound to N-terminal site in the beta-subunit of cyclic nucleotide-gated channel Deposited 2022-06-23 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；308 K；Ionic strength (raw mmCIF value) 1；Pressure 1
NMR sample composition
0.5 mM [U-100% 13C; U-100% 15N] Calmodulin, 0.85 mM Cyclic nucleotide-gated cation channel beta-1, 20 mM [U-100% 2H] TRIS, 1 mM Calcium chloride, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5 mM [U-100% 13C; U-100% 15N] Calmodulin, 0.85 mM Cyclic nucleotide-gated cation channel beta-1, 20 mM [U-100% 2H] TRIS, 1 mM Calcium chloride, 100% D2O | 100% D2O
NMR sample composition
0.5 mM [U-10% 13C; U-100% 15N] Calmodulin, 0.85 mM Cyclic nucleotide-gated cation channel beta-1, 20 mM [U-100% 2H] TRIS, 1 mM Calcium chloride, 100% D2O | 100% D2O
|
Resolution not provided |
| 8DUJ Global map in C1 of RyR1 particles in complex with ImperaCalcin Deposited 2022-07-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric |
Chain C
1–149（149 aa）
Chain F
1–149（149 aa）
Chain I
1–149（149 aa）
Chain L
1–149（149 aa）
|
Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A | CFF CAFFEINE × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 8DVE RyR1 in presence of IpCa-T26E phosphomimetic and activating ligands Deposited 2022-07-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain C
1–149（149 aa）
Chain F
1–149（149 aa）
Chain I
1–149（149 aa）
Chain L
1–149（149 aa）
|
Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A | CFF CAFFEINE × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.84 Å |
| 8EOW Eag Kv channel with voltage sensor in the up conformation Deposited 2022-10-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
7–148（142 aa）
Chain F
7–148（142 aa）
Chain G
7–148（142 aa）
Chain H
7–148（142 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 8EP0 Eag Kv channel with voltage sensor in the intermediate conformation Deposited 2022-10-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
7–148（142 aa）
Chain F
7–148（142 aa）
Chain G
7–148（142 aa）
Chain H
7–148（142 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.90 Å |
| 8EP1 Eag Kv channel with voltage sensor in the down conformation Deposited 2022-10-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
7–148（142 aa）
Chain F
7–148（142 aa）
Chain G
7–148（142 aa）
Chain H
7–148（142 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.40 Å |
| 8FNY Nucleotide-bound structure of a functional construct of eukaryotic elongation factor 2 kinase. Deposited 2022-12-28 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
2–149（148 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；293 K；100 mM Bis-trispropane, 100 mM NaF, 20.5 % w/v PEG-3350 (2protein/1solution)
|
Resolution 2.22 Å R-free 0.229 |
| 8FNY Nucleotide-bound structure of a functional construct of eukaryotic elongation factor 2 kinase. Deposited 2022-12-28 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
2–149（148 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；293 K；100 mM Bis-trispropane, 100 mM NaF, 20.5 % w/v PEG-3350 (2protein/1solution)
|
Resolution 2.22 Å R-free 0.229 |
| 8FO6 Nucleotide-free structure of a functional construct of eukaryotic elongation factor 2 kinase. Deposited 2022-12-29 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
2–149（148 aa）
|
Not recorded | ZN ZINC ION × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.9；293 K；100 mM Bis-trispropane, 200 mM NaF, 17.6% PEG-3350 (2protein 1solution)
|
Resolution 2.55 Å R-free 0.231 |
| 8GM4 Functional construct of the Eukaryotic elongation factor 2 kinase bound to an ATP-competitive inhibitor Deposited 2023-03-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
2–149（148 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 EKI 7-amino-1-cyclopropyl-3-ethyl-2,4-dioxo-1,2,3,4-tetrahydropyrido[2,3-d]pyrimidine-6-carboxamide × 1 ZN ZINC ION × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.2；295.15 K；Cocktail:16.55% PEG-3350, 200 mM NaF, 100 mM BisTris-Propane
Protein sol: 10.3 mg/mL 20 mM Tris pH 7.5, 100 mM NaCl, 3 mM CaCl2, 1mM TCEP, 0.3 mM ADP, 0.3 m Inhibitor , 0.7 % DMSO
2protein/1cocktail (0.2 ul total)
|
Resolution 2.12 Å R-free 0.238 |
| 8GM5 Functional construct of the Eukaryotic elongation factor 2 kinase bound to Calmodulin, ADP and to the A-484954 inhibitor and showing two conformations for the 498-520 loop Deposited 2023-03-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
2–149（148 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 EKI 7-amino-1-cyclopropyl-3-ethyl-2,4-dioxo-1,2,3,4-tetrahydropyrido[2,3-d]pyrimidine-6-carboxamide × 1 ZN ZINC ION × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.5；295.15 K；Cocktail:16.55% PEG-3350, 0.2 M NaF, 100 mM BisTris-Propane
Protein solution: 10.3 mg/mL 20 mM Tris pH 7.5, 100 mM NaCl, 3 mM CaCl2, 1mM TCEP, 1.5 m Inhibitor , 3.1 % DMSO
2protein/1cocktail (0.2 ul total)
|
Resolution 2.12 Å R-free 0.225 |
| 8IJK human KCNQ2-CaM-Ebio1 complex in the presence of PIP2 Deposited 2023-02-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | 7PN N-(1,2-dihydroacenaphthylen-5-yl)-4-fluoranyl-benzamide × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8J00 Human KCNQ2-CaM in complex with CBD Deposited 2023-04-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | P0T cannabidiol × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8J01 Human KCNQ2-CaM in complex with CBD and PIP2 Deposited 2023-04-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain C
1–149（149 aa）
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | P0T cannabidiol × 8 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8J02 Human KCNQ2(F104A)-CaM-PIP2-CBD complex in state II Deposited 2023-04-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain C
1–149（149 aa）
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | P0T cannabidiol × 4 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8J03 Human KCNQ2(F104A)-CaM-PIP2-CBD complex in state I Deposited 2023-04-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
Chain J
1–149（149 aa）
|
Not recorded | P0T cannabidiol × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8J04 Human KCNQ2-CaM-HN37 complex in the presence of PIP2 Deposited 2023-04-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain C
1–149（149 aa）
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | 9MF methyl N-[4-[(4-fluorophenyl)methyl-prop-2-ynyl-amino]-2,6-dimethyl-phenyl]carbamate × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8J05 Human KCNQ2-CaM complex in the presence of PIP2 Deposited 2023-04-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain C
1–149（149 aa）
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8J07 96nm repeat of human respiratory doublet microtubule and associated axonemal complexes Deposited 2023-04-09 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 974 PDB declaration: 974-meric |
Chain W
1–149（149 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 285 MG MAGNESIUM ION × 285 GDP GUANOSINE-5'-DIPHOSPHATE × 289 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.3
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 8JFK PhK holoenzyme in inactive state, muscle isoform Deposited 2023-05-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric |
Chain D
1–149（149 aa）
Chain H
1–149（149 aa）
Chain L
1–149（149 aa）
Chain P
1–149（149 aa）
|
Not recorded | FAR FARNESYL × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8ODZ Cryo-EM structure of a pre-dimerized murine IL-12 complete extracellular signaling complex (Class 1). Deposited 2023-03-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain D
5–149（145 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；HEPES-buffered saline (HBS) with added calcium chloride: 25 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM CaCl
cryo-EM vitrification conditions
Cryogen ETHANE；Leica EM GP2, 5 s. blotting time.
|
Resolution 3.60 Å |
| 8OE0 Cryo-EM structure of a pre-dimerized murine IL-12 complete extracellular signaling complex (Class 2). Deposited 2023-03-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain D
5–149（145 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；HEPES-buffered saline (HBS) with added calcium chloride: 25 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM CaCl
cryo-EM vitrification conditions
Cryogen ETHANE；Leica EM GP2, 5 s. blotting time.
|
Resolution 4.60 Å |
| 8OE4 Cryo-EM structure of a pre-dimerized human IL-23 complete extracellular signaling complex. Deposited 2023-03-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
5–149（145 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；HEPES-buffered saline (HBS) with added calcium chloride: 25 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM CaCl
cryo-EM vitrification conditions
Cryogen ETHANE；Leica EM GP2, 4.5 s. blotting time.
|
Resolution 3.60 Å |
| 8PB1 Cryo-EM structure of a pre-dimerized murine IL-12 complete extracellular signaling complex (Class 1), obtained after local refinement. Deposited 2023-06-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain D
5–149（145 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；HEPES-buffered saline (HBS) with added calcium chloride: 25 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM CaCl
cryo-EM vitrification conditions
Cryogen ETHANE；Leica EM GP2, 5 s. blotting time.
|
Resolution 3.50 Å |
| 8SIK KCNQ1 with voltage sensor in the up conformation Deposited 2023-04-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–149（149 aa）
Chain D
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8SIM KCNQ1 with voltage sensor in the intermediate conformation Deposited 2023-04-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–149（149 aa）
Chain D
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.20 Å |
| 8SIN KCNQ1 with voltage sensor in the down conformation Deposited 2023-04-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–149（149 aa）
Chain D
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.80 Å |
| 8UXL Structure of PKA phosphorylated human RyR2-R420W in the primed state in the presence of calcium and calmodulin Deposited 2023-11-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain I
1–149（149 aa）
Chain J
1–149（149 aa）
Chain K
1–149（149 aa）
Chain L
1–149（149 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 20 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；0.020 mM Calmodulin was added to the final sample
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.12 Å |
| 8UXM Structure of PKA phosphorylated human RyR2-R420W in the open state in the presence of calcium and calmodulin Deposited 2023-11-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain I
1–149（149 aa）
Chain J
1–149（149 aa）
Chain K
1–149（149 aa）
Chain L
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 20 ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；0.020 mM Calmodulin was added to the final sample
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.56 Å |
| 8W4U human KCNQ2-CaM in complex with PIP2 and HN37 Deposited 2023-08-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain C
1–149（149 aa）
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | 9MF methyl N-[4-[(4-fluorophenyl)methyl-prop-2-ynyl-amino]-2,6-dimethyl-phenyl]carbamate × 4 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8X43 human KCNQ2-CaM-Ebio1-S1 complex in the presence of PIP2 Deposited 2023-11-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–149（149 aa）
Chain D
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | 7Q0 N-(4-azanyl-1,2-dihydroacenaphthylen-5-yl)-4-fluoranyl-benzamide × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8XJI Structure of chimeric RyR complex with flubendiamide Deposited 2023-12-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain I
1–148（148 aa）
Chain J
1–148（148 aa）
Chain K
1–148（148 aa）
Chain L
1–148（148 aa）
|
Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A | A1LVX Flubendiamide × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.91 Å |
| 8XKH Structure of chimeric RyR Complex with tetraniliprole Deposited 2023-12-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain I
1–149（149 aa）
Chain J
1–149（149 aa）
Chain K
1–149（149 aa）
Chain L
1–149（149 aa）
|
Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A | ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 A1LV1 tetraniliprole × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.87 Å |
| 8XLF Structure of chimeric RyR Deposited 2023-12-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain I
1–148（148 aa）
Chain J
1–148（148 aa）
Chain K
1–148（148 aa）
Chain L
1–148（148 aa）
|
Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A | CA CALCIUM ION × 4 ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.62 Å |
| 8XLH Structure of chimeric RyR-I4657M/G4819E Deposited 2023-12-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain I
1–149（149 aa）
Chain J
1–149（149 aa）
Chain K
1–149（149 aa）
Chain L
1–149（149 aa）
|
Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A | ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.62 Å |
| 8XO1 Human KCNQ2-CaM in complex with QO-83 Deposited 2023-12-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | A1LWZ ~{N}-[2-azanyl-3-fluoranyl-4-[[4-(trifluoromethyl)phenyl]methylamino]phenyl]-3-cyclopentyl-propanamide × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8XYA hPhK alpha-beta-gamma-delta subcomplex in inactive state Deposited 2024-01-19 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain D
1–149（149 aa）
|
Not recorded | FAR FARNESYL × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8XYB hPhK gamma-delta subcomplex in inactive state Deposited 2024-01-19 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain D
1–149（149 aa）
|
Not recorded | FAR FARNESYL × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8Y40 Structure of chimeric RyR-I4657M/G4819E complex with chlorantraniliprole Deposited 2024-01-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain I
1–149（149 aa）
Chain J
1–149（149 aa）
Chain K
1–149（149 aa）
Chain L
1–149（149 aa）
|
Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A Mutation：E32A, E68A, E105A, E141A | F0U 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.58 Å |
| 9CUI Structure of human full-length ancestral TRPV6 channel in Calmodulin-bound state Deposited 2024-07-26 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain E
1–149（149 aa）
|
Not recorded | Y01 CHOLESTEROL HEMISUCCINATE × 8 CLR CHOLESTEROL × 4 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 43 CA CALCIUM ION × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.42 Å |
| 9CUK Structure of human full-length derived TRPV6 channel in Calmodulin-bound state Deposited 2024-07-26 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain E
1–149（149 aa）
|
Not recorded | Y01 CHOLESTEROL HEMISUCCINATE × 8 CLR CHOLESTEROL × 4 PCW 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 43 CA CALCIUM ION × 7 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.26 Å |
| 9D9Z Structure of human UBR4-KCMF1-CaM E3 ligase complex (Silencing Factor of the Integrated stress response, SiFI) Deposited 2024-08-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
1–149（149 aa）
Chain D
1–149（149 aa）
|
Not recorded | ZN ZINC ION × 20 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9E17 Structure of RyR1 in the primed state in the presence of caffeine (reprocessed/reanalyzed from EMPIAR-10997, 7TZC, EMD-26205) Deposited 2024-10-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain C
1–149（149 aa）
Chain D
1–149（149 aa）
Chain E
1–149（149 aa）
Chain K
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 20 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 ZN ZINC ION × 4 CFF CAFFEINE × 4 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 L9R (2S)-3-(octadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.45 Å |
| 9EL6 Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation F90L Deposited 2024-12-04 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293.15 K；0.1M PCTP (pH8.0), 25% (w/v) PEG 1500
|
Resolution 2.25 Å R-free 0.301 |
| 9EL6 Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation F90L Deposited 2024-12-04 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293.15 K；0.1M PCTP (pH8.0), 25% (w/v) PEG 1500
|
Resolution 2.25 Å R-free 0.301 |
| 9HXW Cryo-EM structure of the human UBR4/KCMF1/CALM1 complex (composite map) Deposited 2025-01-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
|
Not recorded | ZN ZINC ION × 20 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9IXZ human KCNQ2-CaM-Ebio3 Complex in the Presence of PIP2 Deposited 2024-07-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | A1L3D ~{N}-[7-[bis(fluoranyl)methoxy]-1-prop-2-ynyl-indazol-3-yl]-2-propyl-pentanamide × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9J38 human KCNQ5-CaM in apo state Deposited 2024-08-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 9JQI Crystal structure of calmodulin in complex with KN93 (1:1 complex) Deposited 2024-09-27 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–149（149 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 4 KN9 N-[2-[[[3-(4'-Chlorophenyl)-2-propenyl]methylamino]methyl]phenyl]-N-(2-hydroxyethyl)-4'-methoxybenzenesulfonamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；sodium acetate, sodium cacodylate, PEG 8000
|
Resolution 2.10 Å R-free 0.288 |
| 9JQI Crystal structure of calmodulin in complex with KN93 (1:1 complex) Deposited 2024-09-27 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–149（149 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 4 KN9 N-[2-[[[3-(4'-Chlorophenyl)-2-propenyl]methylamino]methyl]phenyl]-N-(2-hydroxyethyl)-4'-methoxybenzenesulfonamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；sodium acetate, sodium cacodylate, PEG 8000
|
Resolution 2.10 Å R-free 0.288 |
| 9JQI Crystal structure of calmodulin in complex with KN93 (1:1 complex) Deposited 2024-09-27 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1–149（149 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 4 KN9 N-[2-[[[3-(4'-Chlorophenyl)-2-propenyl]methylamino]methyl]phenyl]-N-(2-hydroxyethyl)-4'-methoxybenzenesulfonamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；sodium acetate, sodium cacodylate, PEG 8000
|
Resolution 2.10 Å R-free 0.288 |
| 9K8W Crystal structure of the calcium indicator GCaMP6s-BrUS in calcium-bound state Deposited 2024-10-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–149（148 aa）
|
Mutation：A314P/N373D/D391Y/T392R/S394T/R403G Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；288 K；0.2 M Ammonium tartrate dibasic, pH 7.0, 20% PEG 3350
|
Resolution 2.65 Å R-free 0.236 |
| 9K8X Crystal structure of the calcium indicator GCaMP6s-BrUS-145 in calcium-bounded state Deposited 2024-10-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–149（148 aa）
|
Mutation：A313P/N372D/D390Y/T391R/S393T/R402G Non-standard monomer：Yes (specific site not provided by mmCIF) | EDO 1,2-ETHANEDIOL × 6 CA CALCIUM ION × 4 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；288 K；0.2M Sodium thiocyanate, pH 6.9, 20% PEG 3350
|
Resolution 2.05 Å R-free 0.251 |
| 9KUI Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation F142L Deposited 2024-12-04 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–149（149 aa）
|
Mutation：F142L | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298.15 K；0.1M Tris (pH8.5), 0.2 M Trimethylamine N-oxide dihydrate, 25% (w/v) PEG 3350
|
Resolution 2.68 Å R-free 0.275 |
| 9KUO Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation Q136P Deposited 2024-12-04 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–149（149 aa）
|
Mutation：Q136P | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298.15 K；0.1M MMT (pH8.0), 25% (w/v) PEG 1500
|
Resolution 3.13 Å R-free 0.277 |
| 9KUO Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation Q136P Deposited 2024-12-04 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
1–149（149 aa）
|
Mutation：Q136P | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298.15 K；0.1M MMT (pH8.0), 25% (w/v) PEG 1500
|
Resolution 3.13 Å R-free 0.277 |
| 9KUU Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation D130G Deposited 2024-12-04 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–149（149 aa）
|
Mutation：D130G | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298.15 K；0.1 M Tris (pH 8.5), 0.2 M Sodium acetate trihydrate, 30% (w/v) PEG4000
|
Resolution 2.50 Å R-free 0.262 |
| 9KUU Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation D130G Deposited 2024-12-04 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
1–149（149 aa）
|
Mutation：D130G | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298.15 K；0.1 M Tris (pH 8.5), 0.2 M Sodium acetate trihydrate, 30% (w/v) PEG4000
|
Resolution 2.50 Å R-free 0.262 |
| 9KUZ Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation D134H Deposited 2024-12-04 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–149（149 aa）
|
Mutation：D134H | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298.15 K；0.1 M Hepes (pH 7.5), 25% (w/v) PEGMME2000
|
Resolution 2.07 Å R-free 0.268 |
| 9KUZ Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation D134H Deposited 2024-12-04 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain F
1–149（149 aa）
|
Mutation：D134H | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298.15 K；0.1 M Hepes (pH 7.5), 25% (w/v) PEGMME2000
|
Resolution 2.07 Å R-free 0.268 |
| 9KV1 Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation E141G Deposited 2024-12-04 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–149（149 aa）
|
Mutation：E141G | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298.15 K；0.1M MMT (pH9.0), 25% (w/v) PEG 1500
|
Resolution 2.30 Å R-free 0.292 |
| 9KV9 Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation E141G-Ca Deposited 2024-12-04 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–149（149 aa）
|
Mutation：E141G | CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298.15 K；0.1M MMT (pH9.0), 25% (w/v) PEG 1500
|
Resolution 2.05 Å R-free 0.279 |
| 9KVB Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin Deposited 2024-12-04 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298.15 K；0.2 M NH4CH3COO (PH8.5), 27% (w/v) PEG3350
|
Resolution 2.77 Å R-free 0.342 |
| 9MVW Crystal structure of S101F calmodulin - CaM:RM20 analog complex Deposited 2025-01-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–149（149 aa）
|
Mutation：S101F | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.6；295 K；0.1M Sodium Acetate pH 4.6, 25%(w/v) PEG 4000, 20mM CaCl2
|
Resolution 1.58 Å R-free 0.237 |
| 9MXD Human E104A calmodulin:MLCK RM20 complex Deposited 2025-01-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–149（149 aa）
|
Mutation：E104A | CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.6；293 K；0.1M Sodium Acetate pH 4.6, 25%(w/v) PEG 4000, 20mM CaCl2
|
Resolution 1.17 Å R-free 0.178 |
| 9MY3 Structure of Xenopus KCNQ1-CaM in GDN Deposited 2025-01-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–149（149 aa）
Chain D
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.46 Å |
| 9MY4 Structure of Xenopus KCNQ1(E150R/R221E)-CaM with the VSD in the intermediate state Deposited 2025-01-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–149（149 aa）
Chain D
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.53 Å |
| 9NVN Structure of Nanchung-Inactive-Calmodulin in apo state Deposited 2025-03-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain E
1–74（74 aa）
Chain F
1–74（74 aa）
|
Not recorded | 6OU [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate × 12 LBN 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine × 6 CA CALCIUM ION × 6 D39 (2~{S})-2-azanyl-3-[[(2~{R})-3-hexadecanoyloxy-2-[(~{Z})-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-propanoic acid × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.89 Å |
| 9NVO Structure of Nanchung-Inactive-Calmodulin in complex with Nicotinamide Deposited 2025-03-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain E
1–74（74 aa）
Chain F
1–74（74 aa）
|
Not recorded | 6OU [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate × 14 LBN 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine × 6 NCA NICOTINAMIDE × 2 CA CALCIUM ION × 6 D39 (2~{S})-2-azanyl-3-[[(2~{R})-3-hexadecanoyloxy-2-[(~{Z})-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-propanoic acid × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.49 Å |
| 9NVP Structure of Nanchung-Inactive-Calmodulin in complex with Nicotinamide, EDTA Deposited 2025-03-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
|
Not recorded | 6OU [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate × 14 LBN 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine × 6 NCA NICOTINAMIDE × 2 D39 (2~{S})-2-azanyl-3-[[(2~{R})-3-hexadecanoyloxy-2-[(~{Z})-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-propanoic acid × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.85 Å |
| 9NVQ Structure of Nanchung-Inactive-Calmodulin in complex with Afidopyropen and calcium Deposited 2025-03-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain E
1–74（74 aa）
Chain F
1–74（74 aa）
|
Not recorded | 6OU [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate × 12 LBN 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine × 6 A1B32 Afidopyropen × 2 CA CALCIUM ION × 6 D39 (2~{S})-2-azanyl-3-[[(2~{R})-3-hexadecanoyloxy-2-[(~{Z})-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-propanoic acid × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.01 Å |
| 9NVR Structure of Nanchung-Inactive-Calmodulin in complex with Afidopyropen, EDTA Deposited 2025-03-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
|
Not recorded | 6OU [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate × 8 LBN 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine × 2 A1B32 Afidopyropen × 2 D39 (2~{S})-2-azanyl-3-[[(2~{R})-3-hexadecanoyloxy-2-[(~{Z})-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-propanoic acid × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.13 Å |
| 9NWE E3 ligase UBR4-KCMF1-calmodulin complex Deposited 2025-03-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
1–149（149 aa）
Chain D
1–149（149 aa）
|
Not recorded | ZN ZINC ION × 6 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9O48 Cryo-EM structure of the human SK2-4 chimera/calmodulin channel complex in the Ca2+ bound state Deposited 2025-04-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | K POTASSIUM ION × 2 CA CALCIUM ION × 16 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；20 mM Tris pH 8, 150 mM KCl, 2 mM CaCl2, 0.005% GDN, 0.0005% CHS
cryo-EM vitrification conditions
Cryogen ETHANE；5 uL of sample was applied to grids at 4 degree temperature with 100% humidity. After 30 seconds, grids were blotted for 5 seconds with blot force 25 and plunged into liquid ethane.
|
Resolution 3.10 Å |
| 9O51 Cryo-EM structure of the human SK2-4 chimera/calmodulin channel complex in the Ca2+ free state Deposited 2025-04-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | K POTASSIUM ION × 2 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；20 mM Tris pH 8, 150 mM KCl, 5 mM EGTA, 0.005% GDN, 0.0005% CHS
cryo-EM vitrification conditions
Cryogen ETHANE；5 uL of sample was applied to grids at 4 degree temperature with 100% humidity. After 30 seconds, grids were blotted for 5 seconds with blot force 25 and plunged into liquid ethane.
|
Resolution 3.40 Å |
| 9O52 Cryo-EM structure of the human SK2-4 chimera/calmodulin channel complex bound to the bee toxin apamin Deposited 2025-04-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | K POTASSIUM ION × 4 CA CALCIUM ION × 16 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；20 mM Tris pH 8, 150 mM KCl, 2 mM CaCl2, 0.005% GDN, 0.0005% CHS
cryo-EM vitrification conditions
Cryogen ETHANE；5 uL of sample was applied to grids at 4 degree temperature with 100% humidity. After 30 seconds, grids were blotted for 5 seconds with blot force 25 and plunged into liquid ethane.
|
Resolution 3.18 Å |
| 9O53 Cryo-EM structure of the human SK2-4 chimera/calmodulin channel complex bound to a small molecule inhibitor Deposited 2025-04-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | A1B8D N-(2,1,3-benzoxadiazol-4-yl)-3-(4-methoxybenzene-1-sulfonamido)benzamide × 4 K POTASSIUM ION × 4 CA CALCIUM ION × 16 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；20 mM Tris pH 8, 150 mM KCl, 2 mM CaCl2, 0.005% GDN, 0.0005% CHS
cryo-EM vitrification conditions
Cryogen ETHANE；5 uL of sample was applied to grids at 4 degree temperature with 100% humidity. After 30 seconds, grids were blotted for 5 seconds with blot force 25 and plunged into liquid ethane.
|
Resolution 3.30 Å |
| 9O5O Cryo-EM structure of the human SK2-4 chimera/calmodulin channel complex bound to a small molecule activator Deposited 2025-04-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | A1B8G N-(2,1,3-benzoxadiazol-4-yl)-4-(trifluoromethyl)benzamide × 4 K POTASSIUM ION × 2 CA CALCIUM ION × 16 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8；20 mM Tris pH 8, 150 mM KCl, 2 mM CaCl2, 0.005% GDN, 0.0005% CHS
cryo-EM vitrification conditions
Cryogen ETHANE；5 uL of sample was applied to grids at 4 degree temperature with 100% humidity. After 30 seconds, grids were blotted for 5 seconds with blot force 25 and plunged into liquid ethane.
|
Resolution 3.10 Å |
| 9OCW A constitutively active construct of eukaryotic elongation factor 2 kinase Deposited 2025-04-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
78–149（72 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 CA CALCIUM ION × 2 MG MAGNESIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298.15 K；2 ul of protein were mixed with 1 ul of a 18.8% PEG3350 (Hampton Research) and 314 mM magnesium acetate (pH
not adjusted) solution in a 24 wells plate at room temperature
|
Resolution 2.27 Å R-free 0.257 |
| 9PCQ Phosphorylation of a Conserved Aspartate at the Eukaryotic Elongation Factor 2 Kinase Catalytic Site Deposited 2025-06-28 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B00A
3–149（147 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 2 MN MANGANESE (II) ION × 2 ZN ZINC ION × 1 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298.15 K；Protein was mixed 1/1 with 100 mM BisTrisPropane, 80 mM NaF, 20% w/v PEG3350
|
Resolution 2.30 Å R-free 0.243 |
| 9PQH NMR Structure of Ca2+/Calmodulin bound to the GluN1 C0 domain of the NMDA receptor Deposited 2025-07-22 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 4 |
SOLUTION NMR
NMR measurement conditions
pH 7；308 K；Ionic strength (raw mmCIF value) 1；Pressure 1
NMR sample composition
0.4 mM [U-99% 13C; U-99% 15N] Calmodulin, 1.0 mM GluN1 C0, 1 mM Calcium chloride, 20 mM [U-99% 2H] TRIS, 93 % H2O, 7 % [U-2H] D2O, 93% H2O/7% D2O | 93% H2O/7% D2O
NMR sample composition
0.4 mM [U-99% 13C; U-99% 15N] Calmodulin, 1.0 mM GluN1 C0, 1 mM Calcium chloride, 20 mM [U-99% 2H] TRIS, 100 % [U-2H] D2O, 100% D2O | 100% D2O
|
Resolution not provided |
| 9PQI NMR Structure of Ca2+/Calmodulin bound to the GluN2A C0 domain of the NMDA receptor Deposited 2025-07-22 | Different mutation/modification Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 2 |
SOLUTION NMR
NMR measurement conditions
pH 7；308 K；Ionic strength (raw mmCIF value) 1；Pressure 1
NMR sample composition
400 uM [U-100% 13C; U-100% 15N] Calmodulin, 1 mM GluN2, 1 mM Calcium chloride, 20 mM [U-99% 2H] TRIS, 93 % H2O, 7 % [U-2H] D2O, 93% H2O/7% D2O | 93% H2O/7% D2O
NMR sample composition
400 uM [U-100% 13C; U-100% 15N] Calmodulin, 1 mM GluN2, 1 mM Calcium chloride, 20 mM [U-99% 2H] TRIS, 100 % [U-2H] D2O, 100% D2O | 100% D2O
|
Resolution not provided |
| 9QWS Cryo-EM structure of the human UBR4/KCMF1/CALM1 complex (C-term dimer interface focused refinement) Deposited 2025-04-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
|
Not recorded | ZN ZINC ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9QWU Cryo-EM structure of the human UBR4/KCMF1/CALM1 complex (CALM1 focused refinement) Deposited 2025-04-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
|
Not recorded | ZN ZINC ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9QX0 Cryo-EM structure of the human UBR4/KCMF1/CALM1 complex (C-term focused refinement) Deposited 2025-04-15 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain F
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9U7F structure of human KCNQ1-KCNE1-CaM complex Deposited 2025-03-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain B
1–149（149 aa）
Chain E
1–149（149 aa）
Chain H
1–149（149 aa）
Chain K
1–149（149 aa）
|
Not recorded | PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 4 K POTASSIUM ION × 4 CA CALCIUM ION × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9UC8 structure of human KCNQ1-KCNE1-CaM complex with PIP2 Deposited 2025-04-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain B
1–149（149 aa）
Chain E
1–149（149 aa）
Chain H
1–149（149 aa）
Chain K
1–149（149 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 8 K POTASSIUM ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.36 Å |
| 9UJ4 Structure of human KCNQ1-CaM complex Deposited 2025-04-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–149（149 aa）
Chain D
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | K POTASSIUM ION × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 CA CALCIUM ION × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.59 Å |
| 9UPG Cryo-EM structure of human olfactory CNGA2/A4/B1 in CaM-bound closed state Deposited 2025-04-28 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain H
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.87 Å |
| 9VEC structure of human KCNQ1-KCNE1-CaM complex Deposited 2025-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain B
1–149（149 aa）
Chain E
1–149（149 aa）
Chain H
1–149（149 aa）
Chain K
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9VEI structure of human KCNQ1-KCNE1-CaM complex with PIP2 Deposited 2025-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain B
1–149（149 aa）
Chain E
1–149（149 aa）
Chain H
1–149（149 aa）
Chain K
1–149（149 aa）
|
Not recorded | PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 8 CA CALCIUM ION × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 9VEN structure of human KCNQ1-CaM-PIP2 complex with bent conformation Deposited 2025-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–149（149 aa）
Chain D
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 8 PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 9VEO structure of human KCNQ1-CaM-PIP2 complex with straight conformation Deposited 2025-06-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–149（149 aa）
Chain D
1–149（149 aa）
Chain F
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 8 CA CALCIUM ION × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 9VU9 channel D complex with 4 Deposited 2025-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
|
Not recorded | A1ETX (3~{S})-3-(1~{H}-benzimidazol-2-ylamino)-~{N}-(cyanomethyl)-~{N}-methyl-3-[3-(trifluoromethyl)phenyl]propanamide × 1 K POTASSIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.34 Å |
| 9VUA channel A complex with 1 Deposited 2025-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
|
Not recorded | K POTASSIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.23 Å |
| 9VUB channel C complex with 3 Deposited 2025-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 32 CLR CHOLESTEROL × 8 CA CALCIUM ION × 12 A1B92 Rimtuzalcap × 4 K POTASSIUM ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.35 Å |
| 9VUC channel B complex with 2 Deposited 2025-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
|
Not recorded | K POTASSIUM ION × 4 Y7Z UCL1684 × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.96 Å |
| 9WD8 structure of human KCNQ1-KCNE3-CaM complex with two PIP2 Deposited 2025-08-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain B
1–149（149 aa）
Chain E
1–149（149 aa）
Chain H
1–149（149 aa）
Chain K
1–149（149 aa）
|
Not recorded | CA CALCIUM ION × 8 A1BBG (2R)-3-{[(S)-hydroxy{[(1R,2R,3S,4R,5R,6S)-2,3,6-trihydroxy-4,5-bis(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}propane-1,2-diyl di[(9Z)-octadec-9-enoate] × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 9X5J Cryo-EM structure of the human KCNQ2/3 heteromer channel Deposited 2025-10-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
6–148（143 aa）
Chain F
6–148（143 aa）
Chain G
6–148（143 aa）
Chain H
6–148（143 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions | Resolution 3.05 Å |
| 9X65 Cryo-EM structure of the human KCNQ2/3 heteromer channel in the XEN1101-bound open state Deposited 2025-10-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | A1EY8 Azetukalner × 4 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 4 K POTASSIUM ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.19 Å |
| 9XB9 Human KCNQ2-CaM in complex with QO-58 and PIP2 Deposited 2025-10-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 4 A1LVR QO-58 × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9XED Human KCNQ2-CaM in complex with QO-83 and PIP2 Deposited 2025-10-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–149（149 aa）
Chain F
1–149（149 aa）
Chain G
1–149（149 aa）
Chain H
1–149（149 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 4 A1LWZ ~{N}-[2-azanyl-3-fluoranyl-4-[[4-(trifluoromethyl)phenyl]methylamino]phenyl]-3-cyclopentyl-propanamide × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9Y5Q Cryo EM structure of KCa3.1_R355K_I/calmodulin channel in complex with rimtuzalcap Deposited 2025-09-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
3–148（146 aa）
Chain F
3–148（146 aa）
Chain G
3–148（146 aa）
Chain H
3–148（146 aa）
|
Not recorded | K POTASSIUM ION × 1 A1B92 Rimtuzalcap × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.73 Å |
| 9YDZ Cryo EM structure of KCa3.1_R355K_II/calmodulin channel in complex with rimtuzalcap Deposited 2025-09-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
82–148（67 aa）
Fragment：residues 82-148
Chain F
82–148（67 aa）
Fragment：residues 82-148
Chain G
82–148（67 aa）
Fragment：residues 82-148
Chain H
82–148（67 aa）
Fragment：residues 82-148
|
Not recorded | K POTASSIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9YG4 VPS13A/Nt-CaM Deposited 2025-09-27 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions | Resolution 3.38 Å |
| 9YQP Cryo-EM structure of the VPS13C N-terminal region in complex with Calmodulin Deposited 2025-10-15 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions | Resolution 4.10 Å |
| 9YRM CryoEM Structure of VPS13 protein, 1-1390 from C. thermophilum, in complex with calmodulin Deposited 2025-10-16 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions | Resolution 3.75 Å |
| 9YRP Full-length human VPS13C in complex with calmodulin from the CryoEM composite map Deposited 2025-10-16 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–149（149 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions | Resolution 4.13 Å |
| 9ZPO Cryo-EM structure of KCa3.1_I/calmodulin channel in complex with SKA31. Deposited 2025-12-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
3–148（146 aa）
Chain F
3–148（146 aa）
Chain G
3–148（146 aa）
Chain H
3–148（146 aa）
|
Not recorded | A1C3Q naphtho[1,2-d][1,3]thiazol-2-amine × 4 CA CALCIUM ION × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.67 Å |
| 9ZPT Cryo-EM structure of KCa3.1_II/calmodulin channel in complex with SKA31. Deposited 2025-12-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
82–148（67 aa）
Fragment：residues 82-148
Chain F
82–148（67 aa）
Fragment：residues 82-148
Chain G
82–148（67 aa）
Fragment：residues 82-148
Chain H
82–148（67 aa）
Fragment：residues 82-148
|
Not recorded | K POTASSIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.39 Å |
| 9ZRK Cryo-EM structure of KCa3.1_I/calmodulin channel in complex with SKA111. Deposited 2025-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
3–148（146 aa）
Chain F
3–148（146 aa）
Chain G
3–148（146 aa）
Chain H
3–148（146 aa）
|
Not recorded | A1C3U 5-methylnaphtho[1,2-d][1,3]thiazol-2-amine × 4 K POTASSIUM ION × 4 CA CALCIUM ION × 16 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.99 Å |
| 9ZRL Cryo-EM structure of KCa3.1_II/calmodulin channel in complex with SKA111. Deposited 2025-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
82–148（67 aa）
Fragment：residues 82-148
Chain F
82–148（67 aa）
Fragment：residues 82-148
Chain G
82–148（67 aa）
Fragment：residues 82-148
Chain H
82–148（67 aa）
Fragment：residues 82-148
|
Not recorded | K POTASSIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.38 Å |
| 9ZRQ Cryo-EM structure of KCa2.2/calmodulin channel in complex with SKA31. Deposited 2025-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
3–148（146 aa）
Chain F
3–148（146 aa）
Chain G
3–148（146 aa）
Chain H
3–148（146 aa）
|
Not recorded | K POTASSIUM ION × 4 A1C3Q naphtho[1,2-d][1,3]thiazol-2-amine × 4 CA CALCIUM ION × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.77 Å |
| 9ZRR Cryo-EM structure of KCa2.2/calmodulin channel in complex with SKA111. Deposited 2025-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
3–146（144 aa）
Chain F
3–146（144 aa）
Chain G
3–146（144 aa）
Chain H
3–146（144 aa）
|
Not recorded | K POTASSIUM ION × 2 A1C3U 5-methylnaphtho[1,2-d][1,3]thiazol-2-amine × 4 CA CALCIUM ION × 12 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.31 Å |
213 other PDB entries and 278 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | CALM1_HUMAN |
| Isoform | — |
| PDB entities | 2 |
| Chains and sequence ranges | Author chain C； PDBConstruct 1–149； UniProt 1–149 Author chain D； PDBConstruct 1–149； UniProt 1–149 |
