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9zrq

Cryo-EM structure of KCa2.2/calmodulin channel in complex with SKA31.

Method: ELECTRON MICROSCOPY D_max: 133.0 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9zrq
deposition_date	2025-12-20
title	Cryo-EM structure of KCa2.2/calmodulin channel in complex with SKA31.
keywords	;Intermediate conductance calcium-activated potassium channel, Ion channel, Calmodulin binding protein, TRANSPORT PROTEIN, MEMBRANE PROTEIN ;; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	44.34 Å
rg_electron	43.62 Å
i0	1465840000.00
molecular_weight	211470.0 kDa
excluded_volume	205280 Å³
envelope_volume	438460 Å³
shell_volume	81610 Å³
envelope_diameter	142.6 Å
shell_rg	51.42 Å
envelope_rg	41.88 Å
shape_rg	43.65 Å
total_rg	43.84 Å
total_atoms	15961
n_residues	2028
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	133.0 Å
rg_real	43.97 Å
rg_real_error	0.78 Å
i0_real	1.4660e+09
i0_real_error	2.3890e+07
rg_reciprocal	44.34 Å
i0_reciprocal	1466000000.0000
total_estimate	0.8946
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	62.8 Å
skewness	-0.050
kurtosis	-0.547
angular_range	— – 0.1800 Å⁻¹
current_alpha	0.0000
highest_alpha	78600000.0000
n_real_points	37
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.913; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.953; Smooth: 0.933

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4. Crystallography & Experiment

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5. Entities & Polymer Info (6)

Entity 1 polymer

description	Small conductance calcium-activated potassium channel protein 2
formula_weight	41114.8 kDa
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	A,B,C,D
atom_count	15981

IGYKLGHRRALFEKRKRLSDYALIFGMFGIVVMVIETELSWGAYDKASLYSLALKCLISLSTIILLGLIIVYHAREIQLFMVDNGADDWRIAMTYERIFFICLEILVCAIHPIPGNYTFTWTARLAFSYAPSTTTADVDIILSIPMFLRLYLIARVMLLHSKLFTDASSRSIGALNKINFNTRFVMKTLMTICPGTVLLVFSISLWIIAAWTVRACERYHDQQDVTSNFLGAMWLISITFLSIGYGDMVPNTYCGKGVCLLTGIMGAGCTALVVAVVARKLELTKAEKHVHNFMMDTQLTKRVKNAAANVLRETWLIYKNTKLVKKIDHAKVRKHQRKFLQAIHQLRSVKMEQRKLNDQAN

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 2 polymer

description	Calmodulin-1
formula_weight	16521.1 kDa
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	E,F,G,H

DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTA

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Homo sapiens

Entity 3 non-polymer

description	POTASSIUM ION
formula_weight	39.1 kDa
src_method	syn
entity_count	4

Entity 4 non-polymer

description	naphtho[1,2-d][1,3]thiazol-2-amine
formula_weight	200.3 kDa
src_method	syn
entity_count	4

Entity 5 non-polymer

description	CALCIUM ION
formula_weight	40.1 kDa
src_method	syn
entity_count	8

Entity 6 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	20

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.770 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	2.77 Å
resolution_method	—
num_particles	210592

fit	/app/data/saxs_computation/zr/9zrq/9zrq.fit
pddf	/app/data/pr_computation/zr/9zrq/9zrq.pr.out
profile	/app/data/saxs_computation/zr/9zrq/9zrq.dat

Cryo-EM structure of KCa2.2/calmodulin channel in complex with SKA31.

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (6)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 non-polymer

Entity 5 non-polymer

Entity 6 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)