Peptidyl-prolyl cis-trans isomerase FKBP1B
Homo sapiens
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein heterocomplex Heteromer Protein × 12 PDB declaration: dodecameric（12） Consistent with protein copy count | Chain B； UniProt 1–108 Chain E； UniProt 1–108 Chain H； UniProt 1–108 Chain K； UniProt 1–108 | Not recorded | RyR2 × 4 Calmodulin-1 × 4 （P0DP23） ZN ZINC ION × 4 CA CALCIUM ION × 12 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 | ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE | Resolution 4.20 Å |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 6JIU | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1C9H CRYSTAL STRUCTURE OF FKBP12.6 IN COMPLEX WITH RAPAMYCIN Deposited 1999-08-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–108（107 aa）
Fragment：FKBP12.6
|
Not recorded | RAP RAPAMYCIN IMMUNOSUPPRESSANT DRUG × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；298 K；PEG 4000, LITHIUM SULFATE, HEPES BUFFER, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.249 |
| 4C02 Crystal structure of human ACVR1 (ALK2) in complex with FKBP12.6 and dorsomorphin Deposited 2013-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
1–108（108 aa）
|
Not recorded | FLC CITRATE ANION × 8 TAK 6-[4-(2-piperidin-1-ylethoxy)phenyl]-3-pyridin-4-ylpyrazolo[1,5-a]pyrimidine × 1 EDO 1,2-ETHANEDIOL × 13 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.2；1.8M AMMONIUM CITRATE, pH 7.2
|
Resolution 2.17 Å R-free 0.198 |
| 4IQ2 P21 crystal form of FKBP12.6 Deposited 2013-01-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–108（107 aa）
|
Mutation：C22V, C76I | MLA MALONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；2.0 M malonic acid, pH 7.0, 4% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.70 Å R-free 0.251 |
| 4IQ2 P21 crystal form of FKBP12.6 Deposited 2013-01-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
2–108（107 aa）
|
Mutation：C22V, C76I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；2.0 M malonic acid, pH 7.0, 4% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.70 Å R-free 0.251 |
| 4IQC P3121 crystal form of FKBP12.6 Deposited 2013-01-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–108（107 aa）
|
Mutation：C22V, C76I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；62% ammonium sulfate, 0.1 M HEPES, pH 7.5, 4% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.222 |
| 4IQC P3121 crystal form of FKBP12.6 Deposited 2013-01-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
2–108（107 aa）
|
Mutation：C22V, C76I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；62% ammonium sulfate, 0.1 M HEPES, pH 7.5, 4% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.222 |
| 5HKG Total chemical synthesis, refolding and crystallographic structure of a fully active immunophilin: calstabin 2 (FKBP12.6). Deposited 2016-01-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–108（107 aa）
|
Not recorded | RAP RAPAMYCIN IMMUNOSUPPRESSANT DRUG × 1 CL CHLORIDE ION × 3 EDO 1,2-ETHANEDIOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；16 % PEG 8K, 10% Glycerol, 500 mM KCl, 100 mM Hepes
|
Resolution 1.50 Å R-free 0.194 |
| 5L1D Structure of rabbit RyR2 in complex with FKBP12.6 in a closed state (conformation C1) Deposited 2016-07-29 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
2–108（107 aa）
Chain D
2–108（107 aa）
Chain F
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；1x Protease Inhibitor cocktail
cryo-EM vitrification conditions
Cryogen ETHANE；Blot for 2 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 11.00 Å |
| 5T15 Structural basis for gating and activation of RyR1 (30 uM Ca2+ dataset, all particles) Deposited 2016-08-17 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 3.60 Å |
| 5T9M Structure of rabbit RyR1 (Ca2+-only dataset, class 1) Deposited 2016-09-09 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.00 Å |
| 5T9N Structure of rabbit RyR1 (Ca2+-only dataset, class 2) Deposited 2016-09-09 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 3.80 Å |
| 5T9R Structure of rabbit RyR1 (Ca2+-only dataset, class 3) Deposited 2016-09-09 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 5.80 Å |
| 5T9S Structure of rabbit RyR1 (Ca2+-only dataset, class 4) Deposited 2016-09-09 | Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.20 Å |
| 5T9V Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 1) Deposited 2016-09-09 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.40 Å |
| 5TA3 Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 2) Deposited 2016-09-09 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.40 Å |
| 5TAL Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 1&2) Deposited 2016-09-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.30 Å |
| 5TAM Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 4) Deposited 2016-09-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.30 Å |
| 5TAN Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 3) Deposited 2016-09-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.30 Å |
| 5TAP Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, all particles) Deposited 2016-09-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.40 Å |
| 5TAQ Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 3&4) Deposited 2016-09-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.10 Å |
| 5TAS Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 1) Deposited 2016-09-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 6.20 Å |
| 5TAT Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 2) Deposited 2016-09-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.80 Å |
| 5TAU Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 3) Deposited 2016-09-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 6.20 Å |
| 5TAV Structure of rabbit RyR1 (Caffeine/ATP/EGTA dataset, class 4) Deposited 2016-09-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.80 Å |
| 5TAW Structure of rabbit RyR1 (ryanodine dataset, all particles) Deposited 2016-09-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.40 Å |
| 5TAX Structure of rabbit RyR1 (ryanodine dataset, class 1) Deposited 2016-09-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 6.60 Å |
| 5TAY Structure of rabbit RyR1 (ryanodine dataset, class 2) Deposited 2016-09-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.60 Å |
| 5TAZ Structure of rabbit RyR1 (ryanodine dataset, class 3) Deposited 2016-09-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.30 Å |
| 5TB0 Structure of rabbit RyR1 (EGTA-only dataset, all particles) Deposited 2016-09-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.40 Å |
| 5TB1 Structure of rabbit RyR1 (EGTA-only dataset, class 1) Deposited 2016-09-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.60 Å |
| 5TB2 Structure of rabbit RyR1 (EGTA-only dataset, class 2) Deposited 2016-09-11 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.60 Å |
| 5TB3 Structure of rabbit RyR1 (EGTA-only dataset, class 3) Deposited 2016-09-11 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.70 Å |
| 5TB4 Structure of rabbit RyR1 (EGTA-only dataset, class 4) Deposited 2016-09-11 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blotted for 3-4 seconds on both sides with Whatman ashless filter paper, blot force 3, wait time 30 seconds
|
Resolution 4.50 Å |
| 6JGZ Structure of RyR2 (F/P/Ca2+ dataset) Deposited 2019-02-16 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain C
1–108（108 aa）
Chain E
1–108（108 aa）
Chain G
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å |
| 6JH6 Structure of RyR2 (F/A/Ca2+ dataset) Deposited 2019-02-17 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain C
1–108（108 aa）
Chain E
1–108（108 aa）
Chain G
1–108（108 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.80 Å |
| 6JHN Structure of RyR2 (F/C/Ca2+ dataset) Deposited 2019-02-18 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–108（108 aa）
Chain D
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 6JI0 Structure of RyR2 (F/A/C/Ca2+ dataset) Deposited 2019-02-19 | Different oligomeric state Different ligand/ion | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–108（108 aa）
Chain D
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6JI8 Structure of RyR2 (F/apoCaM dataset) Deposited 2019-02-20 | Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain B
1–108（108 aa）
Chain E
1–108（108 aa）
Chain H
1–108（108 aa）
Chain K
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6JII Structure of RyR2 (F/A/C/L-Ca2+/apo-CaM-M dataset) Deposited 2019-02-21 | Different ligand/ion | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
1–108（108 aa）
Chain D
1–108（108 aa）
Chain G
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 CFF CAFFEINE × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6JIY Structure of RyR2 (F/A/C/H-Ca2+/Ca2+CaM dataset) Deposited 2019-02-24 | Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain B
1–108（108 aa）
Chain E
1–108（108 aa）
Chain H
1–108（108 aa）
Chain K
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 20 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6JRR Structure of RyR2 (*F/A/C/L-Ca2+ dataset) Deposited 2019-04-05 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–108（108 aa）
Chain D
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 6JRS Structure of RyR2 (*F/A/C/L-Ca2+/Ca2+-CaM dataset) Deposited 2019-04-05 | Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain B
1–108（108 aa）
Chain E
1–108（108 aa）
Chain H
1–108（108 aa）
Chain K
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 12 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6M2W Structure of RyR1 (Ca2+/Caffeine/ATP/CaM1234/CHL) Deposited 2020-03-01 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain B
2–108（107 aa）
Chain E
2–108（107 aa）
Chain H
2–108（107 aa）
Chain K
2–108（107 aa）
|
Not recorded | CFF CAFFEINE × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 F0U 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6PV6 Functional Pathways of Biomolecules Retrieved from Single-particle Snapshots Deposited 2019-07-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 6W1N Pig Ryanodine Receptor (WT) in 5mM EGTA condition Deposited 2020-03-04 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
2–108（107 aa）
Chain C
2–108（107 aa）
Chain E
2–108（107 aa）
Chain G
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 6WOT Cryo-EM structure of recombinant rabbit Ryanodine Receptor type 1 mutant R164C in complex with FKBP12.6 Deposited 2020-04-25 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.54 Å |
| 6WOU Cryo-EM structure of recombinant mouse Ryanodine Receptor type 2 mutant R176Q in complex with FKBP12.6 in nanodisc Deposited 2020-04-25 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.27 Å |
| 6WOV Cryo-EM structure of recombinant mouse Ryanodine Receptor type 2 wild type in complex with FKBP12.6 Deposited 2020-04-25 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.10 Å |
| 6X32 Wt pig RyR1 in complex with apoCaM, EGTA condition (class 1 and 2, closed) Deposited 2020-05-21 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
2–108（107 aa）
Chain D
2–108（107 aa）
Chain G
2–108（107 aa）
Chain J
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6X33 Wt pig RyR1 in complex with apoCaM, EGTA condition (class 3, open) Deposited 2020-05-21 | Different construct Different ligand/ion Different experimental conditions | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
2–108（107 aa）
Chain D
2–108（107 aa）
Chain G
2–108（107 aa）
Chain J
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6X34 Pig R615C RyR1 EGTA (all classes, open) Deposited 2020-05-21 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
2–107（106 aa）
Chain C
2–107（106 aa）
Chain E
2–107（106 aa）
Chain G
2–107（106 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.70 Å |
| 6X35 Pig R615C RyR1 in complex with CaM, EGTA (class 1, open) Deposited 2020-05-21 | Different construct Different ligand/ion Different experimental conditions | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
2–108（107 aa）
Chain D
2–108（107 aa）
Chain G
2–108（107 aa）
Chain J
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6X36 Pig R615C RyR1 in complex with CaM, EGTA (class 3, closed) Deposited 2020-05-21 | Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain A
2–108（107 aa）
Chain D
2–108（107 aa）
Chain G
2–108（107 aa）
Chain J
2–108（107 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.70 Å |
| 7CF9 Structure of RyR1 (Ca2+/CHL) Deposited 2020-06-24 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
2–108（107 aa）
Chain D
2–108（107 aa）
Chain F
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | CA CALCIUM ION × 4 ZN ZINC ION × 4 F0U 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.70 Å |
| 7JMF Functional Pathways of Biomolecules Retrieved from Single-particle Snapshots - Frame 42 - State 6 (S6) Deposited 2020-07-31 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–108（108 aa）
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 7JMG Functional Pathways of Biomolecules Retrieved from Single-particle Snapshots - Frame 22 - State 2 (S2) Deposited 2020-07-31 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
2–108（107 aa）
Chain F
2–108（107 aa）
Chain H
2–108（107 aa）
Chain J
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 7JMH Functional Pathways of Biomolecules Retrieved from Single-particle Snapshots - Frame 35 - State 4 (S4) Deposited 2020-07-31 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
2–108（107 aa）
Chain F
2–108（107 aa）
Chain H
2–108（107 aa）
Chain J
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 7JMI Functional Pathways of Biomolecules Retrieved from Single-particle Snapshots - Frame 29 - State 3 (S3) Deposited 2020-07-31 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
2–108（107 aa）
Chain F
2–108（107 aa）
Chain H
2–108（107 aa）
Chain J
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 7JMJ Functional Pathways of Biomolecules Retrieved from Single-particle Snapshots - Frame 37 - State 5 (S5) Deposited 2020-07-31 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
2–108（107 aa）
Chain F
2–108（107 aa）
Chain H
2–108（107 aa）
Chain J
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 7M6A High resolution structure of the membrane embedded skeletal muscle ryanodine receptor Deposited 2021-03-25 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
Chain O
1–108（108 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.36 Å |
| 7M6L High resolution structure of the membrane embedded skeletal muscle ryanodine receptor Deposited 2021-03-25 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain F
1–108（108 aa）
Chain H
1–108（108 aa）
Chain J
1–108（108 aa）
Chain O
1–108（108 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.98 Å |
| 7T64 Rabbit RyR1 disease mutant Y523S in complex with FKBP12.6 embedded in lipidic nanodisc in the closed state Deposited 2021-12-13 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 2; blot for 1.0s before plunging
|
Resolution 4.00 Å |
| 7T65 Rabbit RyR1 disease mutant Y523S in complex with FKBP12.6 embedded in lipidic nanodisc in the open state Deposited 2021-12-13 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；Blot force 2; blot for 1.0s before plunging
|
Resolution 4.05 Å |
| 7U9Q Structure of PKA phosphorylated human RyR2 in the closed state Deposited 2022-03-11 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.11 Å |
| 7U9R Structure of PKA phosphorylated human RyR2 in the open state Deposited 2022-03-11 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 XAN XANTHINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.69 Å |
| 7U9T Structure of PKA phosphorylated human RyR2 in the closed state in the presence of Calmodulin Deposited 2022-03-11 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.68 Å |
| 7U9X Structure of PKA phosphorylated human RyR2-R2474S in the closed state Deposited 2022-03-11 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.58 Å |
| 7U9Z Structure of PKA phosphorylated human RyR2-R2474S in the open state Deposited 2022-03-11 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 XAN XANTHINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.29 Å |
| 7UA1 Structure of PKA phosphorylated human RyR2-R2474S in the closed state in the presence of ARM210 Deposited 2022-03-11 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.99 Å |
| 7UA3 Structure of PKA phosphorylated human RyR2-R2474S in the closed state in the presence of Calmodulin Deposited 2022-03-11 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.97 Å |
| 7UA4 Structure of PKA phosphorylated human RyR2-R2474S in the open state in the presence of Calmodulin Deposited 2022-03-11 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 XAN XANTHINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.93 Å |
| 7UA5 Structure of dephosphorylated human RyR2 in the closed state Deposited 2022-03-11 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.83 Å |
| 7UA9 Structure of dephosphorylated human RyR2 in the open state Deposited 2022-03-11 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 XAN XANTHINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；Xanthine was made fresh to avoid aggregation. Xanthine stock solution was 10 mM in NaOH 0.5 N.
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.59 Å |
| 7VML Structure of recombinant RyR2 (EGTA dataset, class 1&2, closed state) Deposited 2021-10-09 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
Chain I
2–108（107 aa）
Chain J
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 7VMM Structure of recombinant RyR2 (EGTA dataset, class 1, closed state) Deposited 2021-10-09 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
Chain I
2–108（107 aa）
Chain J
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 7VMN Structure of recombinant RyR2 (EGTA dataset, class 2, closed state) Deposited 2021-10-09 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
Chain I
2–108（107 aa）
Chain J
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 7VMO Structure of recombinant RyR2 (Ca2+ dataset, class 1, open state) Deposited 2021-10-09 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
Chain I
2–108（107 aa）
Chain J
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 7VMP Structure of recombinant RyR2 (Ca2+ dataset, class 2, open state) Deposited 2021-10-09 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
Chain I
2–108（107 aa）
Chain J
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 7VMQ Structure of recombinant RyR2 (Ca2+ dataset, class 3, open state) Deposited 2021-10-09 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
Chain I
2–108（107 aa）
Chain J
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 7VMR Structure of recombinant RyR2 mutant K4593A (EGTA dataset) Deposited 2021-10-09 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
Chain I
2–108（107 aa）
Chain J
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 7VMS Structure of recombinant RyR2 mutant K4593A (Ca2+ dataset) Deposited 2021-10-09 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
Chain I
2–108（107 aa）
Chain J
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8DTY Recombinant mouse RyR2 triple phosphomimetic mutant S2807D/S2813D/S2030D in complex with FKBP12.6 and nanodisc under closed-state conditions Deposited 2022-07-26 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8DTZ Recombinant mouse RyR2 triple phosphonull mutant S2807A/S2813A/S2030A in complex with FKBP12.6 and nanodisc under closed-state conditions Deposited 2022-07-26 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8DUJ Global map in C1 of RyR1 particles in complex with ImperaCalcin Deposited 2022-07-27 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric |
Chain B
2–108（107 aa）
Chain E
2–108（107 aa）
Chain H
2–108（107 aa）
Chain K
2–108（107 aa）
|
Not recorded | CFF CAFFEINE × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 8DVE RyR1 in presence of IpCa-T26E phosphomimetic and activating ligands Deposited 2022-07-28 | Different construct Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain B
2–108（107 aa）
Chain E
2–108（107 aa）
Chain H
2–108（107 aa）
Chain K
2–108（107 aa）
|
Not recorded | CFF CAFFEINE × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.84 Å |
| 8DVV Recombinant mouse RyR2 triple phosphomimetic mutant S2807D/S2813D/S2030D in complex with FKBP12.6 and nanodisc under open-state conditions Deposited 2022-07-29 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | CA CALCIUM ION × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.68 Å |
| 8SEN Cryo-EM Structure of RyR1 Deposited 2023-04-10 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.49 Å |
| 8SEO Cryo-EM Structure of RyR1 + ATP-gamma-S Deposited 2023-04-10 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.92 Å |
| 8SEP Cryo-EM Structure of RyR1 + ADP Deposited 2023-04-10 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.57 Å |
| 8SEQ Cryo-EM Structure of RyR1 + AMP Deposited 2023-04-10 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | AMP ADENOSINE MONOPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8SER Cryo-EM Structure of RyR1 + Adenosine Deposited 2023-04-10 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | ADN ADENOSINE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.42 Å |
| 8SES Cryo-EM Structure of RyR1 + Adenine Deposited 2023-04-10 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 ADE ADENINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.98 Å |
| 8SET Cryo-EM Structure of RyR1 + cAMP Deposited 2023-04-10 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | CMP ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.42 Å |
| 8UQ2 Structure of human RyR2-S2808D in the subprimed state Deposited 2023-10-23 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.98 Å |
| 8UQ3 Structure of human RyR2-S2808D in the closed state in the presence of ARM210 Deposited 2023-10-23 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.18 Å |
| 8UQ4 Structure of human RyR2-S2808D in the subprimed state in the presence of H2O2/NOC-12/GSH Deposited 2023-10-23 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；30 minute incubation with H2O2/NOC-12/GSH was stopped by flash freezing (vitrobot)
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.64 Å |
| 8UXC Structure of PKA phosphorylated human RyR2-R420Q in the primed state Deposited 2023-11-09 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.86 Å |
| 8UXE Structure of PKA phosphorylated human RyR2-R420Q in the closed state in the presence of ARM210 Deposited 2023-11-09 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.53 Å |
| 8UXF Structure of PKA phosphorylated human RyR2-R420W in the primed state Deposited 2023-11-09 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.13 Å |
| 8UXG Structure of PKA phosphorylated human RyR2-R420W in the closed state in the presence of ARM210 Deposited 2023-11-09 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 KVR 4-[(7-methoxy-2,3-dihydro-1,4-benzothiazepin-4(5H)-yl)methyl]benzoic acid × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.08 Å |
| 8UXH Structure of PKA phosphorylated human RyR2-R420W in the primed state in the presence of calcium Deposited 2023-11-09 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.52 Å |
| 8UXI Structure of PKA phosphorylated human RyR2-R420W in the open state in the presence of calcium Deposited 2023-11-09 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.29 Å |
| 8UXL Structure of PKA phosphorylated human RyR2-R420W in the primed state in the presence of calcium and calmodulin Deposited 2023-11-09 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CA CALCIUM ION × 20 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；0.020 mM Calmodulin was added to the final sample
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.12 Å |
| 8UXM Structure of PKA phosphorylated human RyR2-R420W in the open state in the presence of calcium and calmodulin Deposited 2023-11-09 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain E
1–108（108 aa）
Chain F
1–108（108 aa）
Chain G
1–108（108 aa）
Chain H
1–108（108 aa）
|
Not recorded | CA CALCIUM ION × 20 ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；0.020 mM Calmodulin was added to the final sample
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.56 Å |
| 8XJI Structure of chimeric RyR complex with flubendiamide Deposited 2023-12-21 | Different construct Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | A1LVX Flubendiamide × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.91 Å |
| 8XKH Structure of chimeric RyR Complex with tetraniliprole Deposited 2023-12-23 | Different construct Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 A1LV1 tetraniliprole × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.87 Å |
| 8XLF Structure of chimeric RyR Deposited 2023-12-25 | Different construct Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | CA CALCIUM ION × 4 ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.62 Å |
| 8XLH Structure of chimeric RyR-I4657M/G4819E Deposited 2023-12-26 | Different construct Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.62 Å |
| 8Y40 Structure of chimeric RyR-I4657M/G4819E complex with chlorantraniliprole Deposited 2024-01-29 | Different construct Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: 12-meric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | F0U 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide × 4 ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CFF CAFFEINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.58 Å |
| 9C1E Mink RyR3 in closed conformation Deposited 2024-05-29 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
2–108（107 aa）
Chain C
2–108（107 aa）
Chain E
2–108（107 aa）
Chain G
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 CL CHLORIDE ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；20 mM HEPES pH 7.5, 250 mM NaCl, 2 mM DTT, 0.02% GDN, 5 mM EGTA, 1:1000 diluted protease inhibitor cocktail
cryo-EM vitrification conditions
Cryogen ETHANE；blotted for 3 s (blot force of 7 to 10) using ashless blotting paper (Whatman)
|
Resolution 2.89 Å |
| 9C1F Mink RyR3 in open conformation bound to Ca2+/ATP/caffeine Deposited 2024-05-29 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
2–108（107 aa）
Chain C
2–108（107 aa）
Chain E
2–108（107 aa）
Chain G
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 CA CALCIUM ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 8 CFF CAFFEINE × 4 CL CHLORIDE ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；20 mM HEPES pH 7.5, 250 mM NaCl, 2 mM DTT, 0.02% GDN, 0.03 mM free Ca2+ buffered with 2 mM EGTA, 5 mM ATP, 5 mM caffeine, 1:1000 diluted protease inhibitor cocktail
cryo-EM vitrification conditions
Cryogen ETHANE；blotted for 3 s (blot force of 7 to 10) using ashless blotting paper (Whatman)
|
Resolution 3.22 Å |
| 9CGP RyR1 disease mutant Y523S with FKBP12.6, nanodisc and inhibitor dantrolene in the absence of calcium with refined P1 domain Deposited 2024-06-30 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 U1C Dantrolene × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.34 Å |
| 9CGQ Rabbit RyR1 disease mutant Y523S in complex with FKBP12.6, nanodisc and inhibitor dantrolene in the presence of activating calcium Deposited 2024-06-30 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain E
2–108（107 aa）
Chain F
2–108（107 aa）
Chain G
2–108（107 aa）
Chain H
2–108（107 aa）
|
Not recorded | ZN ZINC ION × 4 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 CA CALCIUM ION × 4 U1C Dantrolene × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.23 Å |
| 9KDF CryoEM structure of Calcineurin-fusion Human endothelin receptor type-B in complex with RES-701-3 Deposited 2024-11-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain F
2–108（107 aa）
|
Not recorded | CA CALCIUM ION × 4 FE FE (III) ION × 1 ZN ZINC ION × 1 FK5 8-DEETHYL-8-[BUT-3-ENYL]-ASCOMYCIN × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9KDG CryoEM structure of Calcineurin-fusion Human endothelin receptor type-B in the ligand-free form Deposited 2024-11-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain F
2–108（107 aa）
|
Not recorded | CA CALCIUM ION × 4 FE FE (III) ION × 1 ZN ZINC ION × 1 FK5 8-DEETHYL-8-[BUT-3-ENYL]-ASCOMYCIN × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.33 Å |
| 9OL4 Cryo-EM Structure of Ryanodine Receptor 1: Drug Bound Open Conformation Deposited 2025-05-12 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–108（108 aa）
Chain H
1–108（108 aa）
Chain N
1–108（108 aa）
Chain T
1–108（108 aa）
|
Not recorded | CFF CAFFEINE × 4 CA CALCIUM ION × 4 117 7-[2-(4-FLUORO-PHENYL)-5-ISOPROPYL-3-PHENYL-4-PHENYLCARBAMOYL-PYRROL-1-YL]- 3,5-DIHYDROXY-HEPTANOIC ACID × 12 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.37 Å |
| 9OL5 Rabbit Ryanodine Receptor 1: Atorvastatin Bound Closed Conformation Deposited 2025-05-12 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–108（108 aa）
Chain H
1–108（108 aa）
Chain N
1–108（108 aa）
Chain T
1–108（108 aa）
|
Not recorded | CA CALCIUM ION × 4 117 7-[2-(4-FLUORO-PHENYL)-5-ISOPROPYL-3-PHENYL-4-PHENYLCARBAMOYL-PYRROL-1-YL]- 3,5-DIHYDROXY-HEPTANOIC ACID × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.56 Å |
| 9OL6 Rabbit Ryanodine Receptor 1: DMSO Control Closed Conformation Deposited 2025-05-12 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–108（108 aa）
Chain H
1–108（108 aa）
Chain N
1–108（108 aa）
Chain T
1–108（108 aa）
|
Not recorded | CA CALCIUM ION × 4 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.11 Å |
| 9PWO Pig Ryanodine Receptor 1 R615C Mutant: Atorvastatin Bound Open Conformation Deposited 2025-08-04 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–108（108 aa）
Chain H
1–108（108 aa）
Chain N
1–108（108 aa）
Chain T
1–108（108 aa）
|
Not recorded | CFF CAFFEINE × 4 CA CALCIUM ION × 4 117 7-[2-(4-FLUORO-PHENYL)-5-ISOPROPYL-3-PHENYL-4-PHENYLCARBAMOYL-PYRROL-1-YL]- 3,5-DIHYDROXY-HEPTANOIC ACID × 12 ZN ZINC ION × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.36 Å |
117 other PDB entries and 119 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | FKB1B_HUMAN |
| Isoform | — |
| PDB entities | 2 |
| Chains and sequence ranges | Author chain B； PDBConstruct 1–108； UniProt 1–108 Author chain E； PDBConstruct 1–108； UniProt 1–108 Author chain H； PDBConstruct 1–108； UniProt 1–108 Author chain K； PDBConstruct 1–108； UniProt 1–108 |
