Current Protein Identity：P14678
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1D3B CRYSTAL STRUCTURE OF THE D3B SUBCOMPLEX OF THE HUMAN CORE SNRNP DOMAIN AT 2.0A RESOLUTION Deposited 1998-12-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–91（91 aa）
Fragment：SM MOTIF
|
Not recorded | GOL GLYCEROL × 4 CIT CITRIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；pH 8.50
|
Resolution 2.00 Å R-free 0.265 |
| 1D3B CRYSTAL STRUCTURE OF THE D3B SUBCOMPLEX OF THE HUMAN CORE SNRNP DOMAIN AT 2.0A RESOLUTION Deposited 1998-12-22 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–91（91 aa）
Fragment：SM MOTIF
|
Not recorded | GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；pH 8.50
|
Resolution 2.00 Å R-free 0.265 |
| 1D3B CRYSTAL STRUCTURE OF THE D3B SUBCOMPLEX OF THE HUMAN CORE SNRNP DOMAIN AT 2.0A RESOLUTION Deposited 1998-12-22 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
1–91（91 aa）
Fragment：SM MOTIF
|
Not recorded | GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；pH 8.50
|
Resolution 2.00 Å R-free 0.265 |
| 1D3B CRYSTAL STRUCTURE OF THE D3B SUBCOMPLEX OF THE HUMAN CORE SNRNP DOMAIN AT 2.0A RESOLUTION Deposited 1998-12-22 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain H
1–91（91 aa）
Fragment：SM MOTIF
|
Not recorded | GOL GLYCEROL × 2 CIT CITRIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；pH 8.50
|
Resolution 2.00 Å R-free 0.265 |
| 1D3B CRYSTAL STRUCTURE OF THE D3B SUBCOMPLEX OF THE HUMAN CORE SNRNP DOMAIN AT 2.0A RESOLUTION Deposited 1998-12-22 | Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain J
1–91（91 aa）
Fragment：SM MOTIF
|
Not recorded | GOL GLYCEROL × 1 CIT CITRIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；pH 8.50
|
Resolution 2.00 Å R-free 0.265 |
| 1D3B CRYSTAL STRUCTURE OF THE D3B SUBCOMPLEX OF THE HUMAN CORE SNRNP DOMAIN AT 2.0A RESOLUTION Deposited 1998-12-22 | Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain L
1–91（91 aa）
Fragment：SM MOTIF
|
Not recorded | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；pH 8.50
|
Resolution 2.00 Å R-free 0.265 |
| 3CW1 Crystal Structure of Human Spliceosomal U1 snRNP Deposited 2008-04-21 | Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: decameric（10） Consistent with all polymers |
Chain A
1–174（174 aa）
Fragment：residues 1-174
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；38-40% MPD, 0.3M KCl, 0.1M MES.KOH, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 5.49 Å |
| 3CW1 Crystal Structure of Human Spliceosomal U1 snRNP Deposited 2008-04-21 | Assembly 2 Protein–RNA Heteromer；Protein × 9 PDB declaration: decameric（10） Consistent with all polymers |
Chain H
1–174（174 aa）
Fragment：residues 1-174
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；38-40% MPD, 0.3M KCl, 0.1M MES.KOH, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 5.49 Å |
| 3CW1 Crystal Structure of Human Spliceosomal U1 snRNP Deposited 2008-04-21 | Assembly 3 Protein–RNA Heteromer；Protein × 9 PDB declaration: decameric（10） Consistent with all polymers |
Chain I
1–174（174 aa）
Fragment：residues 1-174
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；38-40% MPD, 0.3M KCl, 0.1M MES.KOH, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 5.49 Å |
| 3CW1 Crystal Structure of Human Spliceosomal U1 snRNP Deposited 2008-04-21 | Assembly 4 Protein–RNA Heteromer；Protein × 9 PDB declaration: decameric（10） Consistent with all polymers |
Chain J
1–174（174 aa）
Fragment：residues 1-174
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；38-40% MPD, 0.3M KCl, 0.1M MES.KOH, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 5.49 Å |
| 3JCR 3D structure determination of the human*U4/U6.U5* tri-snRNP complex Deposited 2016-01-21 | Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 36-meric（36） Consistent with all polymers |
Chain O
1–240（240 aa）
Chain o
1–240（240 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
20 mM HEPES, pH 7.9, 100 mM KCl, 5 mM MgCl2, 0.1 mM EDTA；pH 7.9；20 mM HEPES, pH 7.9, 100 mM KCl, 5 mM MgCl2, 0.1 mM EDTA
cryo-EM vitrification conditions
Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 7.00 Å |
| 4PJO Minimal U1 snRNP Deposited 2014-05-12 | Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: Undecameric（11） Consistent with all polymers |
Chain B
1–95（95 aa）
|
Not recorded | ZN ZINC ION × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 MG MAGNESIUM ION × 8 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.4；277 K；Minimal U1 snRNP at 4 mg/ml in 300mM KCl, 20mM KHepes, pH 7.5, 5mM MgCl2, 1mM DTT, 0.15 mM polyamine-9 mixed 1:1 with 7% MPD, 180mM KCl, 5mM MgSO4, 50mM NaHepes pH 6.4. Streak seeded.
|
Resolution 3.30 Å R-free 0.255 |
| 4PJO Minimal U1 snRNP Deposited 2014-05-12 | Assembly 2 Protein–RNA Heteromer；Protein × 9 PDB declaration: Undecameric（11） Consistent with all polymers |
Chain b
1–95（95 aa）
|
Not recorded | ZN ZINC ION × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 MG MAGNESIUM ION × 5 K POTASSIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.4；277 K；Minimal U1 snRNP at 4 mg/ml in 300mM KCl, 20mM KHepes, pH 7.5, 5mM MgCl2, 1mM DTT, 0.15 mM polyamine-9 mixed 1:1 with 7% MPD, 180mM KCl, 5mM MgSO4, 50mM NaHepes pH 6.4. Streak seeded.
|
Resolution 3.30 Å R-free 0.255 |
| 4PJO Minimal U1 snRNP Deposited 2014-05-12 | Assembly 3 Protein–RNA Heteromer；Protein × 9 PDB declaration: Undecameric（11） Consistent with all polymers |
Chain P
1–95（95 aa）
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 4 K POTASSIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.4；277 K；Minimal U1 snRNP at 4 mg/ml in 300mM KCl, 20mM KHepes, pH 7.5, 5mM MgCl2, 1mM DTT, 0.15 mM polyamine-9 mixed 1:1 with 7% MPD, 180mM KCl, 5mM MgSO4, 50mM NaHepes pH 6.4. Streak seeded.
|
Resolution 3.30 Å R-free 0.255 |
| 4PJO Minimal U1 snRNP Deposited 2014-05-12 | Assembly 4 Protein–RNA Heteromer；Protein × 9 PDB declaration: Undecameric（11） Consistent with all polymers |
Chain p
1–95（95 aa）
|
Not recorded | ZN ZINC ION × 1 MG MAGNESIUM ION × 3 EOH ETHANOL × 1 CL CHLORIDE ION × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.4；277 K；Minimal U1 snRNP at 4 mg/ml in 300mM KCl, 20mM KHepes, pH 7.5, 5mM MgCl2, 1mM DTT, 0.15 mM polyamine-9 mixed 1:1 with 7% MPD, 180mM KCl, 5mM MgSO4, 50mM NaHepes pH 6.4. Streak seeded.
|
Resolution 3.30 Å R-free 0.255 |
| 4WZJ Spliceosomal U4 snRNP core domain Deposited 2014-11-19 | Assembly 1 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric（8） Consistent with all polymers |
Chain B
1–95（95 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å R-free 0.224 |
| 4WZJ Spliceosomal U4 snRNP core domain Deposited 2014-11-19 | Assembly 10 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric（8） Consistent with all polymers |
Chain BBBB
1–95（95 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å R-free 0.224 |
| 4WZJ Spliceosomal U4 snRNP core domain Deposited 2014-11-19 | Assembly 11 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric（8） Consistent with all polymers |
Chain IIII
1–95（95 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å R-free 0.224 |
| 4WZJ Spliceosomal U4 snRNP core domain Deposited 2014-11-19 | Assembly 12 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric（8） Consistent with all polymers |
Chain PPPP
1–95（95 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å R-free 0.224 |
| 4WZJ Spliceosomal U4 snRNP core domain Deposited 2014-11-19 | Assembly 2 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric（8） Consistent with all polymers |
Chain I
1–95（95 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å R-free 0.224 |
| 4WZJ Spliceosomal U4 snRNP core domain Deposited 2014-11-19 | Assembly 3 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric（8） Consistent with all polymers |
Chain P
1–95（95 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å R-free 0.224 |
| 4WZJ Spliceosomal U4 snRNP core domain Deposited 2014-11-19 | Assembly 4 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric（8） Consistent with all polymers |
Chain BB
1–95（95 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å R-free 0.224 |
| 4WZJ Spliceosomal U4 snRNP core domain Deposited 2014-11-19 | Assembly 5 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric（8） Consistent with all polymers |
Chain II
1–95（95 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å R-free 0.224 |
| 4WZJ Spliceosomal U4 snRNP core domain Deposited 2014-11-19 | Assembly 6 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric（8） Consistent with all polymers |
Chain PP
1–95（95 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å R-free 0.224 |
| 4WZJ Spliceosomal U4 snRNP core domain Deposited 2014-11-19 | Assembly 7 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric（8） Consistent with all polymers |
Chain BBB
1–95（95 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å R-free 0.224 |
| 4WZJ Spliceosomal U4 snRNP core domain Deposited 2014-11-19 | Assembly 8 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric（8） Consistent with all polymers |
Chain III
1–95（95 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å R-free 0.224 |
| 4WZJ Spliceosomal U4 snRNP core domain Deposited 2014-11-19 | Assembly 9 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric（8） Consistent with all polymers |
Chain PPP
1–95（95 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å R-free 0.224 |
| 5MQF Cryo-EM structure of a human spliceosome activated for step 2 of splicing (C* complex) Deposited 2016-12-20 | Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 41-meric（41） Consistent with protein count |
Chain f
1–240（240 aa）
Chain m
1–240（240 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.90 Å |
| 5O9Z Cryo-EM structure of a pre-catalytic human spliceosome primed for activation (B complex) Deposited 2017-06-20 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain X
1–240（240 aa）
Chain f
1–240（240 aa）
Chain m
1–240（240 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 5XJC Cryo-EM structure of the human spliceosome just prior to exon ligation at 3.6 angstrom Deposited 2017-04-30 | Assembly 1 Protein–RNA Heteromer；Protein × 46 PDB declaration: 50-meric（50） Consistent with all polymers |
Chain b
1–231（231 aa）
Chain i
1–231（231 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 11 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 5YZG The Cryo-EM Structure of Human Catalytic Step I Spliceosome (C complex) at 4.1 angstrom resolution Deposited 2017-12-14 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain b
1–229（229 aa）
Chain i
1–229（229 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 7 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 5Z56 cryo-EM structure of a human activated spliceosome (mature Bact) at 5.1 angstrom. Deposited 2018-01-17 | Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain b
1–231（231 aa）
Chain i
1–231（231 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 13 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9；20 mM HEPES-KOH, 150 mM NaCl, 1.5 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.10 Å |
| 5Z57 Cryo-EM structure of the human activated spliceosome (late Bact) at 6.5 angstrom Deposited 2018-01-17 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain b
1–231（231 aa）
Chain i
1–231（231 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 ALA ALANINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 10 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9；20 mM HEPES-KOH, pH 7.9, 150 mM NaCl, 1.5 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.50 Å |
| 5Z58 Cryo-EM structure of a human activated spliceosome (early Bact) at 4.9 angstrom. Deposited 2018-01-17 | Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 46-meric（46） Consistent with all polymers |
Chain b
1–231（231 aa）
Chain i
1–231（231 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9；20 mM HEPES-KOH, pH 7.9, 150 mM NaCl, 1.5 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.90 Å |
| 6AH0 The Cryo-EM Structure of the Precusor of Human Pre-catalytic Spliceosome (pre-B complex) Deposited 2018-08-15 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain U
1–231（231 aa）
Chain f
1–231（231 aa）
Chain i
1–231（231 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.70 Å |
| 6FF7 human Bact spliceosome core structure Deposited 2018-01-03 | Assembly 1 Protein–RNA Heteromer；Protein × 57 PDB declaration: 61-meric（61） Consistent with all polymers |
Chain f
1–240（240 aa）
Chain m
1–240（240 aa）
|
Not recorded | ZN ZINC ION × 11 MG MAGNESIUM ION × 5 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |
| 6QDV Human post-catalytic P complex spliceosome Deposited 2019-01-03 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain b
6–87（82 aa）
Chain k
6–87（82 aa）
|
Not recorded | MG MAGNESIUM ION × 7 K POTASSIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 IHP INOSITOL HEXAKISPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；3 uL sample was applied to the grid, left for 25s, then blotted for 2.5-3.5s and immediately plunged into liquid ethane.
|
Resolution 3.30 Å |
| 6QW6 Structure of the human U5.U4/U6 tri-snRNP at 2.9A resolution. Deposited 2019-03-05 | Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric（39） Consistent with all polymers |
Chain 4b
1–240（240 aa）
Chain 5b
1–240（240 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Wait 30s, blot for 2s to 3s.
|
Resolution 2.92 Å |
| 6QX9 Structure of a human fully-assembled precatalytic spliceosome (pre-B complex). Deposited 2019-03-07 | Assembly 1 Protein–RNA Heteromer；Protein × 65 PDB declaration: 71-meric（71） Consistent with all polymers |
Chain 1b
1–240（240 aa）
Chain 2b
1–240（240 aa）
Chain 4b
1–240（240 aa）
Chain 5b
1–240（240 aa）
|
Not recorded | ZN ZINC ION × 6 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.28 Å |
| 6V4X Cryo-EM structure of an active human histone pre-mRNA 3'-end processing machinery at 3.2 Angstrom resolution Deposited 2019-12-02 | Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: dodecameric（12） Consistent with all polymers |
Chain B
1–95（95 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6Y53 human 17S U2 snRNP low resolution part Deposited 2020-02-24 | Assembly 1 Protein–RNA Heteromer；Protein × 18 PDB declaration: nonadecameric（19） Consistent with all polymers |
Chain m
1–240（240 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.10 Å |
| 6Y5Q human 17S U2 snRNP Deposited 2020-02-25 | Assembly 1 Protein–RNA Heteromer；Protein × 21 PDB declaration: 22-meric（22） Consistent with all polymers |
Chain m
1–240（240 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.10 Å |
| 7A5P Human C Complex Spliceosome - Medium-resolution PERIPHERY Deposited 2020-08-21 | Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 48-meric（48） Consistent with all polymers |
Chain b
1–240（240 aa）
Chain m
1–240（240 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.00 Å |
| 7ABG Human pre-Bact-1 spliceosome Deposited 2020-09-07 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain f
1–240（240 aa）
Chain m
1–240（240 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GTG 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-GUANOSINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.80 Å |
| 7ABI Human pre-Bact-2 spliceosome Deposited 2020-09-07 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain f
1–240（240 aa）
Chain m
1–240（240 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.00 Å |
| 7DVQ Cryo-EM Structure of the Activated Human Minor Spliceosome (minor Bact Complex) Deposited 2021-01-14 | Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 49-meric（49） Consistent with all polymers |
Chain b
1–240（240 aa）
Chain i
1–240（240 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 K POTASSIUM ION × 2 G5J 5'-O-[(S)-hydroxy{[(R)-hydroxy{[(S)-hydroxy(methoxy)phosphoryl]oxy}phosphoryl]oxy}phosphoryl]guanosine × 1 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.89 Å |
| 7EVO The cryo-EM structure of the human 17S U2 snRNP Deposited 2021-05-21 | Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 21-meric（21） Consistent with all polymers |
Chain f
1–240（240 aa）
|
Not recorded | 9B0 [(2~{S},3~{S},4~{E},6~{S},7~{R},10~{R})-3,7-dimethyl-2-[(2~{E},4~{E},6~{R})-6-methyl-6-oxidanyl-7-[(2~{R},3~{R})-3-[(2~{R},3~{S})-3-oxidanylpentan-2-yl]oxiran-2-yl]hepta-2,4-dien-2-yl]-7,10-bis(oxidanyl)-12-oxidanylidene-1-oxacyclododec-4-en-6-yl] 4-cycloheptylpiperazine-1-carboxylate × 1 ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 7QTT Structural organization of a late activated human spliceosome (Baqr, core region) Deposited 2022-01-15 | Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 37-meric（37） Consistent with all polymers |
Chain n
1–240（240 aa）
|
Not recorded | ZN ZINC ION × 12 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9；All solutions were sterile filtered using a 0.22um vacuum filtration unit.
cryo-EM vitrification conditions
Cryogen ETHANE；Volumes of 4 ul of the concentrated sample were applied to one side of glow-discharged UltrAuFoil 200 2/2 grids (Quantifoil) in a Vitrobot Mark IV (FEI) operating at 4 degrees Celsius and 100% humidity. The grids were blotted for 2s with blotting force 5 and immediately frozen by plunging into liquid ethane.
|
Resolution 3.10 Å |
| 7VPX The cryo-EM structure of the human pre-A complex Deposited 2021-10-18 | Assembly 1 Protein–RNA Heteromer；Protein × 31 PDB declaration: 35-meric（35） Consistent with all polymers |
Chain f
1–240（240 aa）
Chain k
1–240（240 aa）
|
Not recorded | ZN ZINC ION × 6 SJT spliceostatin A (form II) × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 7W59 The cryo-EM structure of human pre-C*-I complex Deposited 2021-11-29 | Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 50-meric（50） Consistent with all polymers |
Chain b
1–240（240 aa）
Chain i
1–240（240 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 9 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 7W5A The cryo-EM structure of human pre-C*-II complex Deposited 2021-11-29 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain b
1–240（240 aa）
Chain i
1–240（240 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 9 ZN ZINC ION × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 7W5B The cryo-EM structure of human C* complex Deposited 2021-11-29 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain b
1–240（240 aa）
Chain i
1–240（240 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 9 ZN ZINC ION × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 8C6J Human spliceosomal PM5 C* complex Deposited 2023-01-12 | Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 63-meric（63） Consistent with all polymers |
Chain b
1–240（240 aa）
Chain k
1–240（240 aa）
|
Not recorded | K POTASSIUM ION × 2 MG MAGNESIUM ION × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 8CH6 Structure of a late-stage activated spliceosome (BAqr) arrested with a dominant-negative Aquarius mutant (state B complex). Deposited 2023-02-07 | Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain 8
1–240（240 aa）
Chain n
1–240（240 aa）
|
Not recorded | ZN ZINC ION × 12 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9；All solutions were sterile-filtered using a 0.22um vacuum filtration unit.
cryo-EM vitrification conditions
Cryogen ETHANE；Volumes of 4 ul of the concentrated sample were applied to one side of glow-discharged UltrAuFoil 200 2/2 grids (Quantifoil) in a Vitrobot Mark IV (FEI), operated at 4 degrees Celsius and 100% humidity. The grids were blotted for 2s with blotting force 5 and immediately frozen by plunging into liquid ethane.
|
Resolution 5.90 Å |
| 8H6E Cryo-EM structure of human exon-defined spliceosome in the late pre-B state. Deposited 2022-10-17 | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric（61） Consistent with all polymers |
Chain 2a
1–231（231 aa）
Chain 4a
1–231（231 aa）
Chain 5a
1–231（231 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8H6J Cryo-EM structure of human exon-defined spliceosome in the mature pre-B state. Deposited 2022-10-18 | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric（61） Consistent with all polymers |
Chain 2a
1–231（231 aa）
Chain 4a
1–231（231 aa）
Chain 5a
1–231（231 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.25 Å |
| 8H6K Cryo-EM structure of human exon-defined spliceosome in the mature B state. Deposited 2022-10-18 | Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 63-meric（63） Consistent with all polymers |
Chain 2a
1–231（231 aa）
Chain 4a
1–231（231 aa）
Chain 5a
1–231（231 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8H6L Cryo-EM structure of human exon-defined spliceosome in the early B state. Deposited 2022-10-18 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain 2a
1–231（231 aa）
Chain 4a
1–231（231 aa）
Chain 5a
1–231（231 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 8HK1 The cryo-EM structure of human pre-17S U2 snRNP Deposited 2022-11-24 | Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 21-meric（21） Consistent with all polymers |
Chain f
1–240（240 aa）
|
Not recorded | 9B0 [(2~{S},3~{S},4~{E},6~{S},7~{R},10~{R})-3,7-dimethyl-2-[(2~{E},4~{E},6~{R})-6-methyl-6-oxidanyl-7-[(2~{R},3~{R})-3-[(2~{R},3~{S})-3-oxidanylpentan-2-yl]oxiran-2-yl]hepta-2,4-dien-2-yl]-7,10-bis(oxidanyl)-12-oxidanylidene-1-oxacyclododec-4-en-6-yl] 4-cycloheptylpiperazine-1-carboxylate × 1 ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8I0P The cryo-EM structure of human pre-Bact complex Deposited 2023-01-11 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain a
1–240（240 aa）
Chain m
1–240（240 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8I0R The cryo-EM structure of human Bact-I complex Deposited 2023-01-11 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain a
1–240（240 aa）
Chain m
1–240（240 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8I0S The cryo-EM structure of human Bact-II complex Deposited 2023-01-11 | Assembly 1 Protein–RNA Heteromer；Protein × 46 PDB declaration: 50-meric（50） Consistent with all polymers |
Chain a
1–240（240 aa）
Chain m
1–240（240 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 7 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 8I0T The cryo-EM structure of human Bact-III complex Deposited 2023-01-11 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain a
1–240（240 aa）
Chain m
1–240（240 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 7 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8I0U The cryo-EM structure of human Bact-IV complex Deposited 2023-01-11 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain a
1–240（240 aa）
Chain m
1–240（240 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 7 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8I0V The cryo-EM structure of human post-Bact complex Deposited 2023-01-11 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain a
1–240（240 aa）
Chain m
1–240（240 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 7 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8I0W The cryo-EM structure of human C complex Deposited 2023-01-11 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain a
1–240（240 aa）
Chain i
1–240（240 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8Q7Q Structure of the recycling U5 snRNP bound to chaperones CD2BP2 and TSSC4 (State 2) Deposited 2023-08-16 | Assembly 1 Protein–RNA Heteromer；Protein × 14 PDB declaration: 15-meric（15） Consistent with all polymers |
Chain b
1–240（240 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8Q7V Structure of the recycling U5 snRNP bound to chaperones CD2BP2 and TSSC4 (State 1) Deposited 2023-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 14 PDB declaration: 15-meric（15） Consistent with all polymers |
Chain b
1–240（240 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8Q7W Structure of the recycling U5 snRNP bound to chaperone CD2BP2 (State 3) Deposited 2023-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 14 PDB declaration: 15-meric（15） Consistent with all polymers |
Chain b
1–240（240 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 8Q7X Structure of the recycling U5 snRNP bound to chaperone CD2BP2 (State 4) Deposited 2023-08-17 | Assembly 1 Protein–RNA Heteromer；Protein × 14 PDB declaration: 15-meric（15） Consistent with all polymers |
Chain b
1–240（240 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å |
| 8Q91 Structure of the human 20S U5 snRNP core Deposited 2023-08-19 | Assembly 1 Protein–RNA Heteromer；Protein × 13 PDB declaration: 14-meric（14） Consistent with all polymers |
Chain h
1–240（240 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8QO9 Cryo-EM structure of a human spliceosomal B complex protomer Deposited 2023-09-28 | Assembly 1 Protein–RNA Heteromer；Protein × 66 PDB declaration: 71-meric（71） Consistent with all polymers |
Chain 2b
1–240（240 aa）
Chain 4b
1–240（240 aa）
Chain 5b
1–240（240 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.29 Å |
| 8QXD Cryo-EM structure of the cross-exon pre-B complex Deposited 2023-10-24 | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric（61） Consistent with all polymers |
Chain 2b
1–240（240 aa）
Chain 4b
8–89（82 aa）
Chain 5b
4–89（86 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.60 Å |
| 8QZS Cryo-EM structure of the cross-exon B-like complex Deposited 2023-10-29 | Assembly 1 Protein–RNA Heteromer；Protein × 61 PDB declaration: 68-meric（68） Consistent with all polymers |
Chain 2b
1–240（240 aa）
Chain 4b
1–240（240 aa）
Chain 5b
1–240（240 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 8R08 Cryo-EM structure of the cross-exon pre-B+AMPPNP complex Deposited 2023-10-31 | Assembly 1 Protein–RNA Heteromer；Protein × 64 PDB declaration: 71-meric（71） Consistent with all polymers |
Chain 1b
1–240（240 aa）
Chain 2b
1–240（240 aa）
Chain 4b
1–240（240 aa）
Chain 5b
1–240（240 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.10 Å |
| 8R09 Cryo-EM structure of the cross-exon pre-B+5'ss+ATPgammaS complex Deposited 2023-10-31 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain 2b
1–240（240 aa）
Chain 4b
1–240（240 aa）
Chain 5b
1–240（240 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 8R0A Cryo-EM structure of the cross-exon pre-B+5'ss complex Deposited 2023-10-31 | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 62-meric（62） Consistent with all polymers |
Chain 2b
1–240（240 aa）
Chain 4b
1–240（240 aa）
Chain 5b
1–240（240 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.80 Å |
| 8R0B Cryo-EM structure of the cross-exon pre-B+ATP complex Deposited 2023-10-31 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain 2b
1–240（240 aa）
Chain 4b
1–240（240 aa）
Chain 5b
1–240（240 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å |
| 8R7N Overall structure of the U11 snRNP Deposited 2023-11-26 | Assembly 1 Protein–RNA Heteromer；Protein × 12 PDB declaration: 13-meric（13） Consistent with all polymers |
Chain k
1–240（240 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9；20 mM HEPES-KOH, pH 7.9
200 mM KCl
2 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8RC0 Structure of the human 20S U5 snRNP Deposited 2023-12-05 | Assembly 1 Protein–RNA Heteromer；Protein × 14 PDB declaration: 15-meric（15） Consistent with all polymers |
Chain h
1–240（240 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8RM5 Cryo-EM structure of the cross-exon pre-B+5'ssLNG+ATPyS complex Deposited 2024-01-05 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain 2b
1–240（240 aa）
Chain 4b
1–240（240 aa）
Chain 5b
1–240（240 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.90 Å |
| 8RO2 Integrative Structure of the human intron lariat Spliceosome (ILS'') Deposited 2024-01-11 | Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 40-meric（40） Consistent with all polymers |
Chain b
1–240（240 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 3 IHP INOSITOL HEXAKISPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8Y6O Cryo-EM Structure of the human minor pre-B complex (pre-precatalytic spliceosome) U11 and tri-snRNP part Deposited 2024-02-02 | Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric（46） Consistent with all polymers |
Chain a
1–240（240 aa）
Chain h
1–240（240 aa）
Chain o
1–240（240 aa）
|
Not recorded | MG MAGNESIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen NITROGEN
|
Resolution 3.38 Å |
| 8Y7E Cryo-EM Structure of the human minor pre-B complex (pre-precatalytic spliceosome) U12 snRNP part Deposited 2024-02-04 | Assembly 1 Protein–RNA Heteromer；Protein × 15 PDB declaration: heptadecameric（17） Consistent with all polymers |
Chain i
1–240（240 aa）
|
Not recorded | ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen NITROGEN
|
Resolution 4.66 Å |
| 9FMD Integrative model of the human post-catalytic spliceosome (P-complex) Deposited 2024-06-05 | Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 63-meric（63） Consistent with all polymers |
Chain b
1–240（240 aa）
Chain i
1–240（240 aa）
|
Not recorded | MG MAGNESIUM ION × 6 K POTASSIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 IHP INOSITOL HEXAKISPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9GBW Overall structure of the substrate-bound U11 snRNP Deposited 2024-07-31 | Assembly 1 Protein–RNA Heteromer；Protein × 12 PDB declaration: 14-meric（14） Consistent with all polymers |
Chain k
1–240（240 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9；20 mM HEPES-KOH, pH 7.9
200 mM KCl
2 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 9GC0 3'-lobe of the substrate-bound U11 snRNP Deposited 2024-07-31 | Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 11-meric（11） Consistent with all polymers |
Chain k
1–240（240 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9；20 mM HEPES-KOH, pH 7.9
200 mM KCl
2 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9GCL Structure of the U11 snRNP C-lobe Deposited 2024-08-02 | Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: decameric（10） Consistent with all polymers |
Chain k
1–240（240 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9；20 mM HEPES-KOH, pH 7.9
200 mM KCl
2 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9N96 CRYO-EM STRUCTURE OF human U7 SM RING IN COMPLEX WITH SYMPLEKIN N-TERMINAL DOMAIN Deposited 2025-02-10 | Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: decameric（10） Consistent with all polymers |
Chain B
1–95（95 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.18 Å |
| 9NB1 CRYO-EM STRUCTURE OF human U7 SNRNP WITH MUTANT LSM11 that disrupts contacts with CPSF73 Deposited 2025-02-13 | Assembly 1 Protein–RNA Heteromer；Protein × 11 PDB declaration: 13-meric（13） Consistent with all polymers |
Chain B
1–95（95 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.85 Å |
| 9NH5 CRYO-EM STRUCTURE OF HUMAN U7 SNRNP WITH METHYLATED noncleavable H2A* SUBSTRATE PRE-MRNA (core region) Deposited 2025-02-24 | Assembly 1 Protein–RNA Heteromer；Protein × 11 PDB declaration: 13-meric（13） Consistent with all polymers |
Chain B
1–95（95 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.82 Å |
| 9NH6 CRYO-EM STRUCTURE OF HUMAN U7 SNRNP WITH METHYLATED noncleavable H2A* SUBSTRATE PRE-MRNA (COMPOSITE MAP) Deposited 2025-02-24 | Assembly 1 Protein–RNA Heteromer；Protein × 11 PDB declaration: 13-meric（13） Consistent with all polymers |
Chain B
1–95（95 aa）
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.82 Å |
| 9R8V Cryo-EM structure of the human pre-Bact-OTS complex (whole map) Deposited 2025-05-17 | Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 63-meric（63） Consistent with all polymers |
Chain 2b
1–240（240 aa）
Chain 5b
1–240（240 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.50 Å |