2x0a

MPD-Lysozyme structure at 55.5 keV using a TRIXXEL CsI-aSi based digital imager and the new ESRF U22 undulator source at ID15

Method: X-RAY DIFFRACTION Dmax: 50.9 Å Quality: GOOD

1. Protein Identity and Related Structures Protein Identity & Related Structures

LYSOZYME C

OrganismNot specified

UniProt P00698

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric(1) Consistent with protein copy count Chain A; UniProt 19–147 Not recorded CL CHLORIDE ION × 2 NA SODIUM ION × 2 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 X-RAY DIFFRACTION X-ray crystallization conditions:VAPOR DIFFUSION, HANGING DROP;pH 7;295 K;HANGING DROP METHOD AT 295K. DROP IS 1 MICROL OF 50 MG/ML PROTEIN AND 1 MICROL OF WELL SOLUTION. WELL VOLUME IS 1ML OF 70 % MPD FROM HAMPTON RESEARCH. Resolution 1.52 Å R-free 0.199

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

1320 other PDB entries and 1450 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name LYSC_CHICK
Isoform
PDB entities 1
Chains and sequence ranges Author chain A; PDBConstruct 1–129; UniProt 19–147

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 2x0a

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 2x0a
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2. Structure Basics 2. Structure Basics

Entry ID entry_id2x0a
Deposition date deposition_date2009-12-07
Structure title titleMPD-Lysozyme structure at 55.5 keV using a TRIXXEL CsI-aSi based digital imager and the new ESRF U22 undulator source at ID15
Keywords keywordsHYDROLASE, HIGH ENERGY, RADIATION DAMAGE; HYDROLASE
Experimental Method methodX-RAY DIFFRACTION

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier15.20
Radius of gyration Rg (electron density) rg_electron13.89
Forward intensity I(0) i04726770.00
Molecular weight molecular_weight14539.0 kDa
Excluded volume excluded_volume17755 ų
Envelope volume envelope_volume19436 ų
Hydration-shell volume shell_volume12011 ų
Envelope diameter envelope_diameter52.4
Shell Rg shell_rg19.45
Envelope Rg envelope_rg14.19
Shape Rg shape_rg13.86
Total Rg total_rg15.01
Total atoms total_atoms1012
Residues n_residues129
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 −1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax50.9
Rg (real space) rg_real15.13
Rg uncertainty (real space) rg_real_error0.38
I(0) (real space) i0_real4.7270e+06
I(0) uncertainty (real space) i0_real_error5.5120e+04
Rg (reciprocal space) rg_reciprocal15.13
I(0) (reciprocal space) i0_reciprocal4727000.0000
Solution quality estimate total_estimate0.7852
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks3
Primary peak position r_peak_primary20.0
Skewness Skewness skewness0.238
Kurtosis Kurtosis kurtosis-0.166
Angular range angular_range— – 0.5000 −1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha812100.0000
Real-space data points n_real_points80
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.736; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.997; Smooth: 0.000

5. Crystallography and Experiment 5. Crystallography & Experiment

6. Entities and Polymers Entities & Polymers (5)

7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_idd2x0aa_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.2 — Lysozyme-like
Superfamily Superfamily superfamilyd.2.1 — Lysozyme-like
Family Family familyd.2.1.2 — C-type lysozyme

CATH v4.4 (1 domains)

Domain ID domain_id2x0aA00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology530 — Lysozyme
Homologous superfamily homologous superfamily10

8. Citations (1)

9. Files and Curves (10)