7ynv

Crystal structure of photolysed Hen Egg white LYSOZYME introduced with O-(2-nitrobenzyl)-L-tyrosine

Method: X-RAY DIFFRACTION Dmax: 51.0 Å Quality: GOOD

1. Protein Identity and Related Structures Protein Identity & Related Structures

Lysozyme C

Gallus gallus

UniProt P00698

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric(1) Consistent with protein copy count Chain A; UniProt 19–147 Not recorded NA SODIUM ION × 1 CL CHLORIDE ION × 5 ACT ACETATE ION × 1 X-RAY DIFFRACTION X-ray crystallization conditions:VAPOR DIFFUSION, SITTING DROP;pH 3.6;293 K;28% NaCl, 8% PEG6000, 100 mM Na-acetate (pH3.6) Resolution 1.39 Å R-free 0.192

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

1320 other PDB entries and 1450 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name LYSC_CHICK
Isoform
PDB entities 1
Chains and sequence ranges Author chain A; PDBConstruct 1–129; UniProt 19–147

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 7ynv

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 7ynv
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2. Structure Basics 2. Structure Basics

Entry ID entry_id7ynv
Deposition date deposition_date2022-08-01
Structure title titleCrystal structure of photolysed Hen Egg white LYSOZYME introduced with O-(2-nitrobenzyl)-L-tyrosine
Keywords keywordsnonnatural amino acid caged amino acid o-(2-nitrobenzyl)-L-tyrosine lysozyme cell-free protein synthesis, BIOSYNTHETIC PROTEIN; BIOSYNTHETIC PROTEIN
Experimental Method methodX-RAY DIFFRACTION

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier15.23
Radius of gyration Rg (electron density) rg_electron13.91
Forward intensity I(0) i04861890.00
Molecular weight molecular_weight14592.0 kDa
Excluded volume excluded_volume17705 ų
Envelope volume envelope_volume19472 ų
Hydration-shell volume shell_volume12003 ų
Envelope diameter envelope_diameter51.4
Shell Rg shell_rg19.60
Envelope Rg envelope_rg14.24
Shape Rg shape_rg13.87
Total Rg total_rg15.04
Total atoms total_atoms1012
Residues n_residues129
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 −1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax51.0
Rg (real space) rg_real15.15
Rg uncertainty (real space) rg_real_error0.29
I(0) (real space) i0_real4.8620e+06
I(0) uncertainty (real space) i0_real_error5.2400e+04
Rg (reciprocal space) rg_reciprocal15.16
I(0) (reciprocal space) i0_reciprocal4862000.0000
Solution quality estimate total_estimate0.8729
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary20.0
Skewness Skewness skewness0.235
Kurtosis Kurtosis kurtosis-0.210
Angular range angular_range— – 0.5000 −1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha843300.0000
Real-space data points n_real_points80
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.788; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.997; Smooth: 0.984

5. Crystallography and Experiment 5. Crystallography & Experiment

6. Entities and Polymers Entities & Polymers (5)

7. Fold Classification (SCOP + CATH) 1 domains

CATH v4.4 (1 domains)

Domain ID domain_id7ynvA01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology530 — Lysozyme
Homologous superfamily homologous superfamily10

8. Citations (1)

9. Files and Curves (10)