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7kni

Cryo-EM structure of Triple ACE2-bound SARS-CoV-2 Trimer Spike at pH 5.5

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Spike glycoprotein

Severe acute respiratory syndrome coronavirus 2

UniProt P0DTC2

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Other combination Heteromer Protein 6 其他Polymer 6 Angiotensin-converting enzyme 2 × 3 （Q9BYF1） 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 6 2-acetamido-2-deoxy-beta-D-glucopyranose × 56 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
7b62 7fg7 7my8 7p5g 7p5q 7p5s 7r95 7tpk 7v22 7xiz 9n2l 9wsp

12 assemblies

Homodimer
7wxz 7wxz 7y9n 7zr2

4 assemblies

Homotetramer
6lvn 7owx

2 assemblies

Other homooligomers
6lxt 6lxt 6m1v 6x29 6x2a 6x2b 6x2c 6x45 6xs6 6zgg 6zgh 7a93

103 assemblies; showing the first 12

Heteromeric or nucleic-acid complex
10mu 11hk 11hl 11hn 11hw 6lzg 6m0j 6m17 6vsb 6vw1 6vw1 6vxx

2353 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name SPIKE_SARS2
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–1208； UniProt 1–1208 Author chain B； PDBConstruct 1–1208； UniProt 1–1208 Author chain C； PDBConstruct 1–1208； UniProt 1–1208

Angiotensin-converting enzyme 2

Homo sapiens

UniProt Q9BYF1

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Other combination Heteromer Protein 6 其他Polymer 6 Spike glycoprotein × 3 （P0DTC2） 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 6 2-acetamido-2-deoxy-beta-D-glucopyranose × 56 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
7p55 8hrn 8hru 8jwh 9fmm 9fmm 9spa

7 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1r4l 28kd 28kd 2ajf 2ajf 3d0g 3d0h 3d0h 3d0h 3d0i 3d0i 3d0i

382 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name ACE2_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain D； PDBConstruct 1–597； UniProt 19–615 Author chain E； PDBConstruct 1–597； UniProt 19–615 Author chain F； PDBConstruct 1–597； UniProt 19–615

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id7kni
Deposition date deposition_date2020-11-04
Structure title titleCryo-EM structure of Triple ACE2-bound SARS-CoV-2 Trimer Spike at pH 5.5
Keywords keywordsCOVID, COVID19, SARS-CoV2, ACE2, prefusion, VIRAL PROTEIN-Hydrolase complex; VIRAL PROTEIN/Hydrolase
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

7kni__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

7kni__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

7kni__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）71.52 Å
Rg (electron density)71.38 Å
Total Rg71.26 Å
Atom count39574
Residues4878
Excluded volume703800 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 7kni__assembly_1__model_1 hexameric （6） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (4)

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6. Fold Classification (SCOP + CATH) 3 domains

CATH v4.4 (3 domains)

Domain ID domain_id7kniA01
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology120 — Jelly Rolls
Homologous superfamily homologous superfamily960 — Spike glycoprotein, N-terminal domain
Domain ID domain_id7kniB01
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology120 — Jelly Rolls
Homologous superfamily homologous superfamily960 — Spike glycoprotein, N-terminal domain
Domain ID domain_id7kniC01
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology120 — Jelly Rolls
Homologous superfamily homologous superfamily960 — Spike glycoprotein, N-terminal domain
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7. Citations (1)